Composition for preventing hair loss, promoting hair growth or increasing hair density

Abstract

The use of a composition comprising a N-long-chain acylamino acid and / or a salt thereof in preventing hair loss and / or promoting hair growth and / or increasing hair density. Particularly, the present invention relates to a related composition comprising a N-long-chain acylamino acid and a basic amino acid.

Description

Composition for preventing hair loss, promoting hair growth or increasing hair density Technical Field The present invention relates to the use of a specific composition for preventing hair loss and / or promoting hair growth and / or increasing hair density, and particularly relates to a composition comprising an N-long-chain acylamino acid and / or a salt thereof. Background Art With the accelerating pace of life and increasing workloads, hair loss disorders such as androgenic alopecia (seborrheic alopecia) and alopecia areata are on the rise, with a younger onset. This type of hair loss is detrimental to appearance and aesthetics. Commonly used Western medications for hair loss treatment are minoxidil and finasteride (finasteride inhibits 5α-reductase in the body, thereby suppressing dihydrotestosterone production). However, both medications are prone to relapse after discontinuation and can also cause side effects such as skin irritation, scalp itching, facial and hand hirsutism, dizziness, anorexia, and tachycardia. In addition to medication, cosmetics are currently being studied for preventing hair loss and / or promoting hair growth and / or increasing hair density. Currently, various plant extracts are commonly used, such as Platycladus orientalis leaves, Polygonum multiflorum, Ligustrum lucidum fruit, Chuanxiong rhizome, Cuscuta australis, Gleditsia sinensis, Gleditsia sinensis, Zingiber officinale, Eclipta prostrata, and Herba striatae. Arginine, 2-substituted ketoamides, peptide copper complexes, iron compounds, and pyrimidine derivatives have also been reported to prevent hair loss and / or promote hair growth. L-arginine is reported to increase nitric oxide levels in the body, which the body uses to make nitrogen. Nitric oxide increases blood flow, relaxes blood vessels, and stimulates hair growth. WO1999013717A1 discloses a method for promoting hair growth, comprising delivering a nitric oxide-releasing substance selected from L-arginine, L-arginine salts, and L-arginine derivatives by topical application to selected skin areas where hair growth is desired. An example discloses applying a permeable cream containing L-arginine hydrochloride (15% w / v) and sodium chloride (10% w / v) to bald spots. The cream is applied to the bald areas each night before bed and rubbed in thoroughly for maximum absorption. However, its effectiveness remains limited. CN101596152A discloses a combination of arginine and Centella asiatica extract; EP1040815A1 discloses a combination of L-arginine, ethanol and propylene glycol; US6019976A discloses a combination of serrulata leaf extract, vitamin B6, vitamin B3, zinc salt and 10-15% by weight of L-arginine; DE20315174U1 discloses a shampoo containing arginine; in the hope of enhancing the ability to induce hair growth. US6136860A discloses the use of L-lysine in the preparation of a medicament for preventing and treating human hair loss, but it is in the form of an oral drug. Although the prior art has disclosed various anti-hair loss agents and hair growth promoters, in actual use, it is often impossible to effectively deliver active substances such as L-arginine into the hair follicles, and the actual effect is very limited; or the related side effects are relatively large; or the use is not convenient, and some also require following medical advice. Summary of the Invention The present invention aims to provide a composition capable of preventing hair loss and / or promoting hair growth and / or increasing hair density, which has a significant effect, is very safe, has no side effects, and can be simply applied during bathing and shampooing, facilitating the use by consumers. Specifically, the solution of the present invention is as follows. [1]. Use of a composition comprising N-long chain acyl amino acid and / or its salt for preventing hair loss and / or promoting hair growth and / or increasing hair density. [2]. The use according to [1], wherein the use is for cosmetic purposes. [3]. The use according to [1], wherein the composition is a composition for topical application. [4]. The use according to any one of [1]-[3], wherein the composition comprises N-long chain acyl amino acid and basic amino acid; or comprises a salt formed by N-long chain acyl amino acid and basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt (such as TEA salt) of N-long chain acyl amino acid. [5]. The use according to any one of [1]-[4], wherein the composition further comprises N-long chain acyl amino acid dipeptide and / or its salt. [6]. The use according to any one of [1]-[5], wherein the composition further comprises N-long chain acyl amino acid dipeptide; or a salt formed by N-long chain acyl amino acid dipeptide and basic amino acid; or a sodium salt, potassium salt or ammonium salt (such as TEA salt) of N-long chain acyl amino acid dipeptide. [7]. The use according to any one of [1]-[6], wherein the N-long chain acyl group in the N-long chain acyl amino acid is derived from a saturated or unsaturated straight-chain or branched-chain fatty acid having 8 to 22 carbon atoms; and / or, the amino acid in the N-long chain acyl amino acid is derived from one or more of glycine, alanine, glutamic acid, sarcosine, aspartic acid, leucine, isoleucine, valine, threonine, proline, phenylalanine, arginine, lysine, (methyl)taurine. [8]. For the use according to any one of [1]-[7], the N-long-chain acyl group in the N-long-chain acyl amino acid is selected from one or more of octanoyl, decanoyl, undecanoyl, lauroyl, myristoyl, pentadecanoyl, palmitoyl, stearoyl, oleoyl, linoleoyl, isostearoyl, coconut oil fatty acyl, and palm oil fatty acyl, preferably coconut oil fatty acyl or lauroyl, and most preferably lauroyl; and / or, the amino acid in the N-long-chain acyl amino acid is derived from one or more of alanine, glycine, glutamic acid, sarcosine, arginine, lysine, (methyl)taurine, preferably alanine, and most preferably L-alanine. [9]. For the use according to any one of [4]-[8], the basic amino acid is selected from one or more of arginine, lysine, citrulline, ornithine, creatine, histidine, diaminobutyric acid, and diaminopropionic acid, preferably arginine and / or lysine, and most preferably L-arginine.

[0010] . For the use according to any one of [5]-[9], the N-long-chain acyl group in the N-long-chain acyl amino acid dipeptide is selected from one or more of octanoyl, decanoyl, undecanoyl, lauroyl, myristoyl, pentadecanoyl, palmitoyl, stearoyl, oleoyl, linoleoyl, isostearoyl, coconut oil fatty acyl, and palm oil fatty acyl, preferably coconut oil fatty acyl or lauroyl, and most preferably lauroyl; and / or, the amino acid in the N-long-chain acyl amino acid dipeptide is derived from one or more of alanine, glycine, glutamic acid, sarcosine, arginine, lysine, (methyl)taurine, preferably alanine, and most preferably L-alanine.

[0011] . For the use according to any one of [5]-

[0010] , the N-long-chain acyl amino acid dipeptide is selected from one or more of N-long-chain acylglycylglycine, N-long-chain acylglutamylglutamic acid, N-long-chain acylalanylalanine, and N-long-chain acylsarcosylsarcosine, preferably N-long-chain acylalanylalanine, and most preferably N-lauroyl-L-alany-L-alanine.

[0012] . For the use according to any one of [4]-

[0011] , the basic amino acid is present in an amount that completely or partially neutralizes the N-long-chain acyl amino acid, preferably with a neutralization degree of more than 85% and less than 100%, more preferably with a neutralization degree of more than 88% and less than 96%; or, the basic amino acid is present in an amount that completely or partially neutralizes the N-long-chain acyl amino acid and the N-long-chain acyl amino acid dipeptide, preferably with a neutralization degree of more than 85% and less than 100%, more preferably with a neutralization degree of more than 88% and less than 96%.

[0013] . For the use according to any one of [1]-

[0012] , the N-long-chain acyl amino acid is lauroyl alanine (N-lauroyl-L-alanine); and / or, the N-long-chain acyl amino acid dipeptide is N-lauroyl-L-alanyl-L-alanine; and / or, the N-long-chain acyl amino acid salt is lauroyl alanine arginine salt.

[0014] . For the use according to any one of [1]-

[0013] , the composition is prepared into an aqueous solution, ointment, liniment, gel, cream, powder, emulsion or spray dosage form; and / or, the corresponding product form of the composition is shampoo, hair conditioner, anti-hair loss essence or hair growth cream.

[0015] . For the use according to any one of [1]-

[0014] , the composition is applied to the hair, optionally massaged, allowed to contact the hair for a certain period of time, and then rinsed off with water.

[0016] . A care composition for inducing and / or promoting the growth of keratin fibers and / or slowing down their natural loss and / or increasing their density, the care composition comprising an N-long-chain acyl amino acid and / or its salt; preferably, the care composition comprises an N-long-chain acyl amino acid and a basic amino acid; or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt (such as TEA salt) of an N-long-chain acyl amino acid; more preferably, the N-long-chain acyl amino acid and / or its salt is lauroyl alanine and a basic amino acid, or a salt formed by lauroyl alanine and a basic amino acid, or a sodium salt, potassium salt or ammonium salt of lauroyl alanine.

[0017] . The use of a care composition for inducing and / or promoting the growth of keratin fibers and / or slowing down their natural loss and / or increasing their density, the care composition comprising an N-long-chain acyl amino acid and / or its salt; preferably, the care composition comprises an N-long-chain acyl amino acid and a basic amino acid; or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt (such as TEA salt) of an N-long-chain acyl amino acid; more preferably, the care composition comprises lauroyl alanine and a basic amino acid, or a salt formed by lauroyl alanine and a basic amino acid, or a sodium salt, potassium salt or ammonium salt of lauroyl alanine.

[0018] . The care composition / use according to

[0016] or

[0017] , wherein the composition further comprises an N-long-chain acyl amino acid dipeptide; or a salt formed by an N-long-chain acyl amino acid dipeptide and a basic amino acid; or a sodium salt, potassium salt or ammonium salt (such as a TEA salt) of an N-long-chain acyl amino acid dipeptide; preferably, the care composition further comprises lauroyl alanyl alanine; or a salt formed by lauroyl alanyl alanine and a basic amino acid; or a sodium salt, potassium salt or ammonium salt of lauroyl alanyl alanine.

[0019] . The care composition / use according to

[0016] or

[0017] , wherein the N-long-chain acyl amino acid, basic amino acid, and N-long-chain acyl amino acid dipeptide are as defined in any one of the foregoing [7]-

[0013] .

[0020] . The care composition according to

[0016] , wherein the care composition is prepared into an aqueous solution, ointment, liniment, gel, cream, powder, emulsion or spray dosage form; and / or, the product form corresponding to the care composition is shampoo, hair conditioner, anti-hair loss essence or hair growth cream.

[0021] . An anti-hair loss shampoo, anti-hair loss essence or hair growth cream, characterized by comprising the care composition according to any one of

[0016] -

[0020] .

[0022] . A non-therapeutic beauty method, which comprises topically applying a care composition to the skin or hair of a human or animal, the composition comprising an N-long-chain acyl amino acid and / or its salt; the non-therapeutic beauty method is the activation, moisturization, prevention of hair loss or stimulation of hair growth of the skin / hair of a human or animal.

[0023] . The beauty method according to

[0022] , wherein the composition comprises an N-long-chain acyl amino acid and a basic amino acid; or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt (such as a TEA salt) of an N-long-chain acyl amino acid.

[0024] . The method according to

[0022] or

[0023] , wherein the composition further comprises an N-long-chain acyl amino acid dipeptide; or a salt formed by an N-long-chain acyl amino acid dipeptide and a basic amino acid; or a sodium salt, potassium salt or ammonium salt (such as a TEA salt) of an N-long-chain acyl amino acid dipeptide.

[0025] . The method according to any one of

[0022] -

[0024] , wherein the N-long-chain acyl amino acid, basic amino acid, and N-long-chain acyl amino acid dipeptide are as defined in any one of the foregoing [7]-

[0013] .

[0026] . According to the method described in any one of

[0022] -

[0025] , the composition is prepared into an aqueous solution, ointment, liniment, gel, cream, powder, emulsion or spray dosage form; and / or, the corresponding product form of the composition is shampoo, hair conditioner, anti-hair loss essence or hair growth paste.

[0027] . Use of a composition comprising N-long chain acyl amino acid and / or its salt in the preparation of a medicament for preventing hair loss and / or promoting hair growth and / or increasing hair density.

[0028] . According to the use described in

[0027] , the composition comprises N-long chain acyl amino acid and basic amino acid; or comprises a salt formed by N-long chain acyl amino acid and basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt (such as TEA salt) of N-long chain acyl amino acid.

[0029] . According to the use described in any one of

[0027] -

[0028] , the composition further comprises N-long chain acyl amino acid dipeptide; or a salt formed by N-long chain acyl amino acid dipeptide and basic amino acid; or a sodium salt, potassium salt or ammonium salt (such as TEA salt) of N-long chain acyl amino acid dipeptide.

[0030] . According to the use described in any one of

[0023] -

[0025] , the N-long chain acyl amino acid, basic amino acid, N-long chain acyl amino acid dipeptide are defined as in any one of the foregoing [7]-

[0013] .

[0031] . According to any one of [1]-

[0030] , the weight percentage content of N-long chain acyl amino acid and / or its salt in the composition is preferably above 1 wt%, and more preferably above 5 wt%. Compared with the prior art, the present invention has beneficial technical effects: 1. The present invention unexpectedly discovers that a composition comprising N-long chain acyl amino acid and / or its salt can be used for preventing hair loss and / or promoting hair growth and / or increasing hair density. 2. The combination of basic amino acid and N-long chain acyl amino acid exhibits excellent effects of preventing hair loss and / or promoting hair growth and / or increasing hair density. 3. The combination of lauroyl alanine and arginine or lauroyl alanine arginine salt has stronger effects on preventing hair loss and / or promoting hair growth and / or increasing hair density than other N-long chain acyl amino acids and / or their salts. Description of the Drawings Figure 1 is a graph of the inhibition rate of 5α-reductase activity of the samples in Example I. Detailed Embodiments In one embodiment, there is provided a use of a composition comprising an N-long-chain acyl amino acid and / or a salt thereof for preventing hair loss and / or promoting hair growth and / or increasing hair density, particularly for cosmetic use. The term "cosmetic use" is intended to mean a use that is not a therapeutic use, a pharmaceutical use, or a dermatological use, i.e., a use that does not require a therapeutic treatment and is intended for healthy skin. The term "healthy skin" is intended to mean skin that is described by an expert in the field (such as a dermatologist) as non-pathological, i.e., skin that does not exhibit any infection, scar, skin disease or skin disorder (such as candidiasis, impetigo, psoriasis, eczema, acne or dermatitis), wound or injury and / or other skin diseases and / or androgenetic alopecia. The term "preventing hair loss" means preventing, inhibiting or delaying the shedding of hair. The composition may be a composition for topical application. The composition may be prepared in dosage forms such as aqueous solutions, ointments, liniments, gels, creams, powders, emulsions or sprays. For specific product forms, for example, it may be a shampoo, a hair conditioner, an anti-hair loss essence or a hair growth cream. For the application method, for example, the composition may be applied to the hair, optionally massaged, allowed to contact the hair for a certain period of time, and then rinsed off with water. For the N-long-chain acyl amino acid, the N-long-chain acyl in the N-long-chain acyl amino acid is derived from a saturated or unsaturated straight-chain or branched-chain fatty acid having 8 to 22 carbon atoms. Further, the N-long-chain acyl in the N-long-chain acyl amino acid is selected from one or more of octanoyl, decanoyl, undecanoyl, lauroyl, myristoyl, pentadecanoyl, palmitoyl, stearoyl, oleoyl, linoleoyl, isostearoyl, coconut oil fatty acyl, palm oil fatty acyl, preferably coconut oil fatty acyl or lauroyl, and most preferably lauroyl. The amino acid in the N-long-chain acyl amino acid is derived from one or more of glycine, alanine, glutamic acid, sarcosine, aspartic acid, leucine, isoleucine, valine, threonine, proline, phenylalanine, arginine, lysine. Further, the amino acid in the N-long-chain acyl amino acid is derived from one or more of alanine, glycine, glutamic acid, sarcosine, arginine, lysine, (methyl)taurine, preferably alanine, and most preferably L-alanine. The N-long-chain acyl (methyl)taurine described in the present invention refers to N-long-chain acyl methyl taurine or N-long-chain acyl taurine. As an example, the N-long-chain acyl amino acid may be selected from cocoyl alanine, lauroyl alanine, cocoyl sarcosine, lauroyl sarcosine, lauroyl glutamate, cocoyl glutamate, oleoyl glutamate, cocoyl glycine, lauroyl glycine, stearoyl glutamate, etc. Lauroyl alanine and lauroyl sarcosine are preferred, and N-lauroyl-L-alanine is particularly preferred. The synthesis process of N-long-chain acyl amino acids can refer to the general synthesis methods of N-acyl amino acid surfactants, which are divided into direct methods and indirect methods. The direct synthesis method from fatty acid raw materials includes enzymatic catalysis synthesis and dehydration condensation. The indirect synthesis methods include fatty acyl chloride acylation, fatty nitrile hydrolysis acylation, fatty acid anhydride acylation, amide carbonylation reaction, etc. More preferably, it is prepared by reacting fatty acyl chloride with the amino group of amino acids (Schotten-Baumann condensation reaction or Schotten-Baumann reaction). A typical preparation process suitable for the present invention is as follows: amino acids and sodium hydroxide are dissolved in water or a mixed solution of water and acetone to obtain an amino acid salt solution; then, lauroyl chloride and sodium hydroxide solution are slowly added dropwise to the amino acid salt solution, controlling the pH of the reaction system. After the addition is completed, post-treatment of the product is carried out. Representative methods are disclosed in CN1798821A, US6703517B2, CN102875409B, JPH0570418A, etc. For the post-treatment of N-long-chain acyl amino acid products, conventional recrystallization, water washing, drying, etc. can be used. Preferably, the post-treatment includes the following steps: mixing the crude N-long-chain acyl amino acid with a solvent, optionally stirring, controlling the temperature T of the mixed system above the melting point of the long-chain fatty acid and below the melting point of the N-long-chain acyl amino acid. The solvent is water, an organic solvent, or a mixed solution of water and an organic solvent; after the temperature of the system is controlled, a solid-liquid separation operation is carried out. Related methods have been disclosed by the present inventors in CN202210867760.7, PCT / CN2022 / 107270, and the relevant content is incorporated herein by reference. For N-long-chain acyl amino acids and / or their salts, it refers to a combination of one or more of N-long-chain acyl amino acids and N-long-chain acyl amino acid salts. Among them, the N-long-chain acyl amino acid salt is formed by N-long-chain acyl amino acid and a base. The base is selected from one or more of inorganic bases, organic amines, and basic amino acids; the inorganic base is selected from one or more of sodium hydroxide, potassium hydroxide, sodium carbonate, and potassium carbonate, preferably sodium hydroxide or potassium hydroxide; the organic amines are selected from amines, alkanolamines, etc. For basic amino acids, they are selected from one or more of arginine, lysine, citrulline, ornithine, creatine, histidine, diaminobutyric acid, and diaminopropionic acid. Arginine and / or lysine are preferred, and L-arginine is most preferred. In addition, the composition may further comprise an N-long-chain acyl amino acid dipeptide or a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or a sodium salt, potassium salt, or ammonium salt of an N-long-chain acyl amino acid dipeptide. N-long-chain acyl amino acid dipeptides are a known concept. Long-chain acyl amino acid dipeptides refer to long-chain acylaminoacyl amino acids, typically such as long-chain acylglycylglycine, long-chain acylglutamylglutamic acid, long-chain acylalanylalanine, long-chain acylsarcosylsarcosine, etc. For example, see CN100448968C, PCT / CN2022 / 107270, etc. N-long-chain acyl amino acid dipeptides can be prepared by the Schotten-Baumann reaction or the like using an acyl chloride derived from an N-long-chain acyl amino acid and an amino acid; or by reacting an amino acid dipeptide such as glutamylglutamic acid with an acyl chloride. It is preferably obtained by using the related preparation method disclosed in PCT / CN2022 / 107270, and the relevant content is incorporated herein by reference. The N-long-chain acyl group in the N-long-chain acyl amino acid dipeptide is selected from one or more of octanoyl, decanoyl, undecanoyl, lauroyl, myristoyl, pentadecanoyl, palmitoyl, stearoyl, oleoyl, linoleoyl, isostearoyl, coconut oil fatty acyl, and palm oil fatty acyl. Preferably, it is coconut oil fatty acyl or lauroyl, and most preferably lauroyl; The amino acid in the N-long-chain acyl amino acid dipeptide is derived from one or more of alanine, glycine, glutamic acid, sarcosine, arginine, lysine, (methyl)taurine. Preferably, it is L-alanine. The present invention preferably uses N-long-chain acyl-L-alanine dipeptides, especially N-lauroyl-L-alanyl-L-alanine. Preferably, the basic amino acid is present in an amount that completely or partially neutralizes the N-long-chain acyl amino acid, preferably with a neutralization degree of more than 85% and less than 100%, more preferably with a neutralization degree of more than 88% and less than 96%; or the basic amino acid is present in an amount that completely or partially neutralizes the N-long-chain acyl amino acid and the N-long-chain acyl amino acid dipeptide, preferably with a neutralization degree of more than 85% and less than 100%, more preferably with a neutralization degree of more than 88% and less than 96%. In one embodiment, it relates to a care composition for inducing and / or promoting the growth of keratin fibers and / or slowing down their natural loss and / or increasing their density. Of course, it also relates to the use of the care composition for inducing and / or promoting the growth of keratin fibers and / or slowing down their natural loss and / or increasing their density. The nursing composition contains N-long-chain acyl amino acids and / or their salts. Preferably, the N-long-chain acyl amino acids and / or their salts are the main surfactants in the nursing composition. The so-called main surfactant means that, in terms of weight percentage, the content of this surfactant is the highest among all surfactants. Preferably, the weight percentage of the main surfactant among all surfactants exceeds 50 wt%; for example, it exceeds 55 wt%, 60 wt%, 65 wt%, 70 wt%, 75 wt%, 80 wt%. Except for the main surfactant, other surfactants are co-surfactants. The so-called co-surfactant means that, compared with the main surfactant, the amount of the co-surfactant used is less than that of the main surfactant and it plays an auxiliary role in cleaning. The co-surfactant is not necessary, but of course, a small amount of co-surfactant is preferably added in most applications. The nursing composition contains N-long-chain acyl amino acids and basic amino acids, or contains salts formed by N-long-chain acyl amino acids and basic amino acids, or contains sodium salts, potassium salts or ammonium salts of N-long-chain acyl amino acids. The term "keratin fiber" means hair, eyebrows and / or eyelashes. Preferably, the keratin fiber is hair. The nursing composition may further contain N-long-chain acyl amino acid dipeptides, or salts formed by N-long-chain acyl amino acid dipeptides and basic amino acids, or sodium salts, potassium salts, ammonium salts of N-long-chain acyl amino acid dipeptides. For the definitions and preferred types of the N-long-chain acyl amino acids, basic amino acids, and N-long-chain acyl amino acid dipeptides, refer to the definitions in the foregoing embodiments, and they will not be elaborated here. The nursing composition may be a composition for topical application. The composition can be prepared into dosage forms such as aqueous solutions, ointments, liniments, gels, creams, powders, emulsions or sprays. For specific product forms, for example, it can be shampoo, hair conditioner, anti-hair loss essence or hair growth cream, etc. For the application method, for example, the composition can be applied to the hair, optionally massaged, allowed to contact the hair for a certain period of time, and then rinsed off with water. In one embodiment, it relates to a non-therapeutic cosmetic method, which comprises topically applying a nursing composition to the skin or hair of a human or an animal, and the composition contains N-long-chain acyl amino acids and / or their salts; the non-therapeutic cosmetic method is for the activation, moisturization, prevention of hair loss or stimulation of hair growth of the skin / hair of humans or animals. Preferably, it is a cosmetic method for preventing, inhibiting or delaying hair loss. Furthermore, the composition contains N-long-chain acyl amino acids and basic amino acids, or contains salts formed by N-long-chain acyl amino acids and basic amino acids, or contains sodium salts, potassium salts or ammonium salts of N-long-chain acyl amino acids. The composition may further comprise an N-long-chain acyl amino acid dipeptide, or a salt formed by an N-long-chain acyl amino acid dipeptide and a basic amino acid, or a sodium salt, potassium salt, or ammonium salt of an N-long-chain acyl amino acid dipeptide. For the definitions and preferred types of the N-long-chain acyl amino acid, basic amino acid, and N-long-chain acyl amino acid dipeptide, refer to the definitions in the foregoing embodiments, and they will not be elaborated herein. The composition may be a composition for topical application. The composition may be prepared into dosage forms such as aqueous solutions, ointments, liniments, gels, creams, powders, emulsions, or sprays. For specific product forms, for example, it may be shampoo, hair conditioner, anti-hair loss essence, or hair growth cream, etc. For the application method, for example, the composition may be applied to the hair, optionally massaged, allowed to contact the hair for a certain period of time, and then rinsed off with water. In one embodiment, it relates to the use of a composition comprising an N-long-chain acyl amino acid and / or its salt in the preparation of a medicament for preventing hair loss and / or promoting hair growth and / or increasing hair density. Furthermore, the composition comprises an N-long-chain acyl amino acid and a basic amino acid, or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid, or comprises a sodium salt, potassium salt, or ammonium salt of an N-long-chain acyl amino acid. The composition may further comprise an N-long-chain acyl amino acid dipeptide, or a salt formed by an N-long-chain acyl amino acid dipeptide and a basic amino acid, or a sodium salt, potassium salt, or ammonium salt of an N-long-chain acyl amino acid dipeptide. For the definitions and preferred types of the N-long-chain acyl amino acid, basic amino acid, and N-long-chain acyl amino acid dipeptide, refer to the definitions in the foregoing embodiments, and they will not be elaborated herein. In the above embodiments, the content of the N-long-chain acyl amino acid and / or its salt in the composition is preferably above 1 wt%, more preferably above 5 wt%, for example, above 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 wt%. In the above embodiments, the composition of the present invention may optionally further comprise other components, such as fragrances, dyes, surfactants, thickeners, moisturizers, chelating agents, extracts, amino acids, nucleic acids, vitamins, enzymes, anti-inflammatory agents, bactericides, antioxidants, ultraviolet absorbers, antiperspirants, pH regulators, pearlescent agents, etc. In each embodiment, for the detailed situations, type selections, dosage selections, etc. that are not discussed, in the case of no conflict in content, reference may be made to the relevant guidance of other embodiments. For the sake of brevity, each embodiment is not elaborated one by one. The present invention will be further described below in conjunction with the accompanying drawings and embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalents also fall within the scope defined by the appended claims of this application. Example 1 Test purpose Androgenetic alopecia (AGA) is caused by the conversion of testosterone (T) into more active dihydrotestosterone (DHT) under the action of 5α-reductase. It is sensitive to hair follicles and affects hair follicle development, resulting in hair loss. By analyzing whether the sample can inhibit 5α-reductase and thus inhibit the production of DHT, this experiment can be used as a basis for judging whether it has the effect of preventing hair loss. 2 Test items Anti-hair loss efficacy test - 5α-reductase inhibition rate. 3 Test materials 3.1 Main reagents 1) Type II 5α-reductase: Extracted from rat prostate tissue and stored at -80°C. 2) Positive control (PC): 1 μM finasteride solution. 3) Blank control (BC): Phosphate buffer solution (PBS). 3.2 Main equipment High performance liquid chromatography (HPLC). 4 Test methods 4.1 Preparation of type II 5α-reductase: Take 5 male SD rats, fast overnight without water deprivation, and then sacrifice them by CO 2 asphyxiation. Take out the prostate and cut it into pieces on an ice table. Make a 1:5 homogenate with pre-cooled buffer in a glass homogenizer at 0°C, centrifuge at 3000 g for 10 min, take the supernatant, centrifuge at 10000 g for 30 min, take the supernatant and aliquot it into EP tubes, 1 mL per tube, which is the enzyme extract, and store it in a -80°C refrigerator. 4.2 Determination of type II 5α-reductase activity: In a 2 mL reaction system, there are: 0.25 mL of phosphate buffer, 0.70 mL of enzyme extract, 0.1 mL of testosterone solution (0.5 mg / mL), 0.35 mL of NADPH solution (1 mg / mL), and 0.6 mL of (10% ethanol in the complete reaction group, finasteride in the positive control group, and sample solution in the sample group). In the control tube, the enzyme extract is replaced with phosphate buffer, and 2.0 mL of dichloromethane is added to terminate the reaction; in the sample tube, the reaction is carried out at 37 °C for 30 min. After the reaction, 2.0 mL of dichloromethane is added to stop the reaction. After shaking for 1 min, it is centrifuged at 5000 r / min for 10 min. The upper aqueous phase is removed, about 1.0 mL of the organic phase is taken out and evaporated to dryness. The residue is dissolved in 1.0 mL of methanol, and the peak area of testosterone (T) is determined by high performance liquid chromatography. Each reaction is set with 3 parallel tubes. Calculation of inhibition rate: 5 Detection results I. Test results carried out in Zhongqing Daily Chemical Sample preparation (TA): 6% lauroyl alanine + 3.84% arginine + 0.2% benzoic acid + 0.25% essence + the balance of water (TO 100). Then, it is diluted with PBS buffer solution in the experimental system to obtain a reaction concentration of 30% (V / V) to obtain the sample. The results of 5α-reductase activity inhibition rate are shown in Table 1 and Figure 1. Table 1 Summary table of 5α-reductase activity inhibition rate results Group System concentration (%,V / V) 5α-reductase activity inhibition rate (%) SD p-value Blank control (BC) / / / / Negative control (NC) / 0.00 0.50 / Positive control (PC) 1 72.21 0.37 0.005** Sample 30 69.77 0.43 0.002** Note: When performing statistical analysis by the t-test method, compared with the complete reaction group, the significance of the sample group and the PC group is indicated by *, p-value < 0.05 is indicated by *, and p-value < 0.01 is indicated by **. Compared with the NC group, at the administration concentration of 1 μM, the inhibition rate of 5α-reductase in the PC group is 72.21%, showing a statistical difference (p < 0.01), indicating that the positive control test in this study is effective. Compared with the NC group, at the system concentration of 30% (V / V), the inhibition rate of 5α-reductase in the sample is 69.77%, showing a statistical difference (p < 0.01). II. Test Results Conducted by Guangzhou Huadai Biotechnology Positive Control 1: Finasteride Weigh 0.0372 g, add absolute ethanol to 1 mL. Take 0.005 mL and add it to 4.995 mL of absolute ethanol to prepare a stock solution. Then take 0.005 mL and add it to 0.495 mL of absolute ethanol to prepare a finasteride solution with a concentration of 1 μmol / L. Positive Control 2: Minoxidil Weigh 0.50 g of minoxidil, add 1 g of propylene glycol, and add 8.50 g of 75% alcohol. Sample Preparation: ① First, add 83.90 g of water, then add 10 g of lauroyl alanine. Raise the temperature to 80 - 85 °C and maintain this temperature. Continue to add 6.10 g of arginine and stir to dissolve to obtain the lauroyl alanine arginine salt sample LA-Arg(10%) (calculated based on lauroyl alanine, the lauroyl alanine content in the sample is 10 wt%). Dilute the aforementioned sample with PBS buffer solution to obtain the sample LA-Arg(5%) with a lauroyl alanine content of 5 wt%. ② First, add 88.6 g of ultrapure water, then add 1.4 g of sodium hydroxide. Raise the temperature to 80 - 85 °C, dissolve and maintain this temperature. Continue to add 10 g of lauroyl alanine and stir to dissolve to obtain the sodium lauroyl alanine sample LA-Na(10%) (calculated based on lauroyl alanine, the lauroyl alanine content in the sample is 10 wt%). Dilute the aforementioned sample with PBS buffer solution to obtain the sample LA-Na(5%) with a lauroyl alanine content of 5 wt%. ③ First, add 90 g of water, then add 10 g of arginine to obtain the sample Arg(10%) (the arginine content in the sample is 10 wt%). Dilute the aforementioned sample with PBS buffer solution to obtain the sample Arg(5%) with an arginine content of 5 wt%. ④ First, add 84.76 g of water, then add 10 g of lauroyl glutamate. Raise the temperature to 80 - 85 °C and maintain this temperature. Continue to add 5.24 g of arginine and stir to dissolve to obtain the lauroyl glutamate arginine salt sample Glu-Arg(10%) (the lauroyl glutamate content in the sample is 10 wt%). Dilute the aforementioned sample with PBS buffer solution to obtain the sample Glu-Arg(5%) with a lauroyl glutamate content of 5 wt%. ⑤ First, add 83.23 g of water, and then add 10 g of lauroyl glycine. Raise the temperature to 80 - 85 °C, maintain this temperature, and continue to add 6.77 g of arginine. Stir to dissolve to obtain the lauroyl glycine arginine salt sample Gly-Arg(10%) (the content of lauroyl glycine in the sample is 10 wt%). The aforementioned sample is diluted with PBS buffer solution to obtain the sample Gly-Arg(5%) with a lauroyl glycine content of 5 wt%. The results of the 5α-reductase activity inhibition rate are shown in Table 2. Table 2 Summary of the results of the 5α-reductase activity inhibition rate Group5α-reductase activity inhibition rate (%)Blank control group / complete reaction group0.00 ± 0.51Positive control 1: Finasteride79.79 ± 0.33*Positive control 2: Minoxidil17.82 ± 0.24*Sample LA-Arg(10%)73.24 ± 0.26*Sample LA-Arg(5%)90.06 ± 0.34*Sample LA-Na(10%)67.26 ± 0.83*Sample LA-Na(5%)48.69 ± 0.37*Sample Arg(10%)94.08 ± 0.41*Sample Arg(5%)18.83 ± 0.44*Sample Glu-Arg(10%)49.65 ± 0.15*Sample Glu-Arg(5%)42.39 ± 0.13*Sample Gly-Arg(10%)56.89 ± 0.56*Sample Gly-Arg(5%)48.97 ± 0.14* *: Compared with the complete reaction group, the difference was statistically significant (P < 0.05). In addition, those skilled in the art can understand that although some of the embodiments described herein include certain features included in other embodiments rather than other features, the combination of the features of different embodiments is meant to be within the scope of the present invention and forms different embodiments. For example, in the claims, any one of the claimed embodiments can be used in any combination.

Claims

Use of a composition comprising an N-long-chain acyl amino acid and / or its salt for preventing hair loss and / or promoting hair growth and / or increasing hair density.

2. The use according to claim 1, characterized in that, The use is for cosmetic purposes; and / or, the composition is a composition for topical application.

3. The use according to claim 1 or 2, characterized in that, The composition comprises an N-long-chain acyl amino acid and a basic amino acid; or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt of an N-long-chain acyl amino acid.

4. The use according to any one of claims 1 to 3, characterized in that, The composition further comprises an N-long-chain acyl amino acid dipeptide and / or its salt.

5. The use according to any one of claims 1 to 4, characterized in that, The N-long-chain acyl in the N-long-chain acyl amino acid is derived from a saturated or unsaturated straight-chain or branched-chain fatty acid having 8 to 22 carbon atoms. Preferably, the N-long-chain acyl is selected from cocoyl or lauroyl, and most preferably lauroyl; and / or, the amino acid in the N-long-chain acyl amino acid is derived from one or more of glycine, alanine, glutamic acid, sarcosine, aspartic acid, leucine, isoleucine, valine, threonine, proline, phenylalanine, arginine, lysine, (methyl)taurine. Preferably, it is alanine, and most preferably L-alanine.

6. The use according to claim 3, characterized in that, The basic amino acid is selected from one or more of arginine, lysine, citrulline, ornithine, creatine, histidine, diaminobutyric acid, diaminopropionic acid. Preferably, it is arginine and / or lysine, and most preferably L-arginine.

7. The use according to any one of claims 1 to 6, characterized in that, The composition comprises lauroyl alanine and arginine, or comprises a salt of lauroyl alanine and arginine.

8. A care composition for inducing and / or promoting the growth of keratin fibers and / or slowing down their natural loss and / or increasing their density, characterized in that, The care composition comprises an N-long-chain acyl amino acid and / or its salt; the N-long-chain acyl amino acid and / or its salt is lauroyl alanine and a basic amino acid, or a salt formed by lauroyl alanine and a basic amino acid, or a sodium salt, potassium salt or ammonium salt of lauroyl alanine.

9. The care composition according to claim 8, characterized in that, The care composition further comprises lauroyl alanyl alanine; or a salt formed by lauroyl alanyl alanine and a basic amino acid; or a sodium salt, potassium salt, ammonium salt of lauroyl alanyl alanine.

10. The care composition according to claim 8 or 9, characterized in that, The basic amino acid is selected from one or more of arginine, lysine, citrulline, ornithine, creatine, histidine, diaminobutyric acid, diaminopropionic acid. Preferably, it is arginine and / or lysine, and most preferably L-arginine.

11. The nursing composition according to claim 8, wherein The N-long-chain acyl amino acid and / or its salt is the main surfactant in the care composition. Preferably, the weight percentage content of lauroyl alanine and / or its salt in all surfactants exceeds 60 wt%, and more preferably exceeds 70 wt%.

12. An anti-hair loss shampoo, anti-hair loss essence or hair growth cream, characterized in that, A care composition according to any one of claims 8-11 is included.

13. A non-therapeutic cosmetic method, which comprises topically applying a care composition to the skin or hair of a human or an animal, characterized in that, The care composition comprises an N-long-chain acyl amino acid and / or its salt; the non-therapeutic cosmetic method is the activation, moisturization, prevention of hair loss or stimulation of hair growth of human or animal skin / hair.

14. The beauty method according to claim 13, characterized in that, The care composition comprises an N-long-chain acyl amino acid and a basic amino acid; or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt of an N-long-chain acyl amino acid.

15. The beauty method according to claim 13, characterized in that, The care composition further comprises an N-long-chain acyl amino acid dipeptide and / or its salt.

16. The beauty method according to any one of claims 13-15, characterized in that the N-long-chain acyl group in the N-long-chain acyl amino acid is derived from a saturated or unsaturated straight-chain or branched-chain fatty acid having 8 to 22 carbon atoms. Preferably, the N-long-chain acyl group is selected from a cocoyl group or a lauroyl group, and most preferably the lauroyl group; and / or the amino acid in the N-long-chain acyl amino acid is derived from one or more of glycine, alanine, glutamic acid, sarcosine, aspartic acid, leucine, isoleucine, valine, threonine, proline, phenylalanine, arginine, lysine, (methyl)taurine, preferably alanine, and most preferably L-alanine.

17. The beauty method according to claim 14, characterized in that, The basic amino acid is selected from one or more of arginine, lysine, citrulline, ornithine, creatine, histidine, diaminobutyric acid, diaminopropionic acid, preferably arginine and / or lysine, and most preferably L-arginine.

18. Use of a composition comprising an N-long-chain acyl amino acid and / or its salt in the preparation of a medicament for preventing hair loss and / or promoting hair growth and / or increasing hair density.

19. The use according to claim 18, wherein The composition comprises an N-long-chain acyl amino acid and a basic amino acid; or comprises a salt formed by an N-long-chain acyl amino acid and a basic amino acid; or comprises a sodium salt, potassium salt or ammonium salt of an N-long-chain acyl amino acid.

20. The use according to claim 18, characterized in that, The composition further comprises an N-long-chain acyl amino acid dipeptide and / or its salt.

21. The use according to any one of claims 18 to 20, characterized in that, the N-long-chain acyl group in the N-long-chain acyl amino acid is derived from a saturated or unsaturated straight-chain or branched-chain fatty acid having 8 to 22 carbon atoms. Preferably, the N-long-chain acyl group is selected from a cocoyl group or a lauroyl group, and most preferably the lauroyl group; and / or the amino acid in the N-long-chain acyl amino acid is derived from one or more of glycine, alanine, glutamic acid, sarcosine, aspartic acid, leucine, isoleucine, valine, threonine, proline, phenylalanine, arginine, lysine, (methyl)taurine, preferably alanine, and most preferably L-alanine.

22. The use according to claim 19, wherein, The basic amino acid is selected from one or more of arginine, lysine, citrulline, ornithine, creatine, histidine, diaminobutyric acid, diaminopropionic acid, preferably arginine and / or lysine, and most preferably L-arginine.

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