Anti-hair loss and hair growth liquid containing collagen carbon quantum dots and preparation method of anti-hair loss and hair growth liquid

Abstract

The invention discloses anti-hair loss and hair growth liquid containing collagen carbon quantum dots and a preparation method of the anti-hair loss and hair growth liquid. The anti-hair loss and hair growth liquid is prepared from the following raw materials in percentage by weight: 0.0001 to 0.1 percent of collagen carbon quantum dots, 1 to 40 percent of nutrition repairing agent, 0.1 to 10 percent of penetration enhancer, 0.01 to 2 percent of protective agent, a proper amount of pH (Potential of Hydrogen) regulator and the balance of purified water. Through the biological activity of the collagen carbon quantum dots and the synergistic effect of multiple components, hair follicle cell proliferation and differentiation are promoted, and the damaged hair follicle tissue structure is repaired; hair follicles are stimulated to be converted from a rest period to a growth period, and the hair growth period is prolonged; the composition can improve the scalp microenvironment, enhance the absorption of nutrient substances and reduce the inflammatory response around hair follicles, so that the alopecia problem is improved, the effects of densifying and blackening hair are achieved, and the composition has wide application prospects and is beneficial to popularization and application.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and in particular to a hair loss prevention and hair growth liquid containing collagen carbon quantum dots and a preparation method thereof. Background Art

[0002] With the accelerating pace of society and increasing stress, hair loss is becoming increasingly common. Currently, there are a wide variety of anti-hair loss and hair growth products on the market, primarily categorized as chemical, natural extract, and bioactive ingredient products. Chemical products, such as minoxidil, can cause some users to experience adverse reactions such as scalp irritation and allergies. Long-term use can also lead to hirsutism and drug dependence. Natural extract products lack the stability of their active ingredients, making them difficult to effectively penetrate the stratum corneum. Bioactive ingredient products are expensive and easily inactivated, severely limiting their practical application.

[0003] Collagen carbon quantum dots are rich in functional groups such as amino and carboxyl groups on their surface. They can scavenge reactive oxygen free radicals to protect hair follicle cells, activate the Wnt pathway to promote stem cell proliferation, and inhibit inflammatory signaling pathways to prevent inflammation-mediated hair follicle degeneration. Their nanoparticle size penetrates the scalp's stratum corneum barrier, enhancing nutrient penetration. From cell activation to microenvironmental repair, they offer a new direction for the innovation of anti-hair loss and hair growth products. Given their excellent anti-hair loss and hair growth effects, biocompatibility, water solubility, and cell membrane permeability, there is an urgent need to develop an anti-hair loss and hair growth solution containing collagen carbon quantum dots and a preparation method to address these technical challenges.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] The purpose of the present invention is to provide an anti-hair loss and hair growth liquid containing collagen carbon quantum dots and a preparation method thereof. Through the synergistic effect of the biological activity of collagen carbon quantum dots and multiple components, the hair follicle cell proliferation and differentiation are promoted, and the damaged hair follicle tissue structure is repaired; the hair follicles are stimulated to transform from the resting phase to the growth phase, thereby extending the hair growth cycle; the scalp microenvironment is improved, the absorption of nutrients is enhanced, and the inflammatory response around the hair follicles is reduced, thereby improving the problem of hair loss and achieving the effect of increasing hair density and darkening. The invention has broad application prospects and is conducive to promotion and application.

[0006] In order to achieve the above objectives, the present invention provides an anti-hair loss and hair growth liquid containing collagen carbon quantum dots, which includes the following raw materials in weight percentage: 0.0001-0.1% collagen carbon quantum dots, 1-40% nutritional repair agent, 0.1-10% penetration enhancer, 0.01-2% protective agent, an appropriate amount of pH adjuster, and purified water to 100%.

[0007] Preferably, the nutritional repair agent is one or more of collagen, sodium hyaluronate, vitamin B, vitamin C, vitamin E, arginine, lysine, ceramide, caffeine, tea polyphenols, ubiquinone, menthol, plant extract, stem cell extract, and exosomes.

[0008] Preferably, the penetration enhancer is one or more of oleic acid, linoleic acid, ethyl oleate, isopropyl myristate, menthol, limonene or propylene glycol, glycerin, polyethylene glycol, laurocaprol, polysorbate 80, DMSO, salicylic acid or ethanol.

[0009] Preferably, the protective agent is one or more of vitamin E, tea polyphenols, ascorbic acid, cyclodextrin, glycerol, EDTA, phenoxyethanol, polysorbate 20, chlorphenesin, and trehalose.

[0010] Preferably, the pH regulator is one or more of sodium hydroxide, sodium bicarbonate, triethanolamine, acetic acid, lactic acid, sodium citrate, glycine, arginine, salicylic acid, fruit acid, phosphoric acid, and boric acid.

[0011] The present invention also provides a method for preparing the above-mentioned anti-hair loss and hair growth liquid containing collagen carbon quantum dots, comprising the following steps: S1: Carbonizing, purifying, and freeze-drying a collagen sponge to obtain collagen carbon quantum dot powder, wherein the particle size of the collagen carbon quantum dot powder is 2-20 nm; S2: Weigh the nutritional repair agent, permeation enhancer, and protective agent in proportion, add purified water and stir to mix, homogenize for 0.5-5 hours, and then adjust the pH to 5.0-6.5; S3: Add the collagen carbon quantum dot powder prepared in S1, mix homogenously for 0.5-2 h, and then filter with a 0.22 μm filter membrane. Collect the filtered sterile solution, use a filling machine to package the liquid in a packaging bottle, and use it by spraying or applying.

[0012] Preferably, the specific steps of S1 are: SS1: Weigh 0.1-50 g of collagen sponge into a crucible and heat it in a box furnace at 200-300°C for 0.5-5 h; SS2: Return the SS1 sample to room temperature, add 1-100 g of purified water, grind and stir for 0.5-3 h, collect the mixture, centrifuge at 5000-30000 × g for 10-60 min, and collect the supernatant; SS3: Filter the supernatant collected from SS2 through a 5-20 μm filter membrane, then through a 0.22 μm filter membrane. Transfer the collected filtrate into a 100-5000 Da dialysis bag and dialyze with magnetic stirring for 12-72 h. Change the water 3-5 times during the purification process. Collect the solution in the bag after the dialysis is completed. SS4: The solution obtained in SS3 was freeze-dried / low-temperature dried at a temperature of -40-40°C and a vacuum degree of 10-100 Pa for 10-120 h to obtain collagen carbon quantum dot powder.

[0013] The present invention provides a hair loss prevention and growth liquid containing collagen carbon quantum dots and a preparation method thereof, which have the following beneficial effects.

[0014] 1. This invention leverages the bioactivity of collagen carbon quantum dots and the synergistic effects of multiple ingredients to promote hair follicle cell proliferation and differentiation, repairing damaged follicle tissue structure; stimulate the transition of hair follicles from the resting phase to the growing phase, extending the hair growth cycle; improve the scalp microenvironment, enhance nutrient absorption, and reduce inflammation around hair follicles, thereby alleviating hair loss and achieving the effect of thickening and darkening hair.

[0015] 2. This invention incorporates collagen carbon quantum dots into the formulation of an anti-hair loss and hair growth serum. Collagen carbon quantum dots possess excellent biocompatibility, water solubility, and cell membrane permeability. They can scavenge reactive oxygen free radicals to protect hair follicle cells, activate the Wnt pathway to promote stem cell proliferation, and inhibit inflammatory signaling pathways to prevent inflammation-mediated hair follicle degeneration. Their nanoscale particle size penetrates the scalp's stratum corneum barrier, enhancing nutrient penetration. From cell activation to microenvironmental repair, these dots offer a new direction for the innovation of anti-hair loss and hair growth products.

[0016] 3. The formula of this invention contains nutritional and restorative agents that replenish nutrients needed for hair follicle growth and promote metabolism. Ingredients such as collagen and sodium hyaluronate enhance the scalp's ability to retain moisture and repair damaged hair follicles. Ingredients such as vitamin C and tea polyphenols delay scalp aging by neutralizing free radicals and inhibiting inflammation. Niacinamide and caffeine accelerate local blood circulation, improving nutrient absorption by hair follicles. These nutritional and restorative agents comprehensively improve the scalp environment through moisturizing, repairing, anti-aging, and promoting circulation, achieving comprehensive benefits and promoting hair growth.

[0017] 4. The formula of this invention contains a penetration enhancer, which reduces skin barrier resistance and enhances the transdermal absorption efficiency of active ingredients. It also modulates stratum corneum swelling by altering skin moisture content, further optimizing the permeation pathway. Some ingredients, such as ethanol and propylene glycol, act as solvents, increasing the solubility of active ingredients and synergistically promoting permeation. Ultimately, this allows active substances, such as the nutrient-repairing agent, to reach the site of action more efficiently, enhancing the overall efficacy of the hair growth solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a flow chart of the preparation method of a hair loss prevention and hair growth liquid containing collagen carbon quantum dots provided by the present invention; Figure 2The collagen carbon quantum dot powder prepared in Example 1 of the present invention was dissolved in purified water at a concentration of 0.01%, and exhibited fluorescence properties under ultraviolet light, where (a) was under natural light and (b) was under ultraviolet light. DETAILED DESCRIPTION

[0019] The present invention will be further described below with reference to specific embodiments and accompanying drawings to facilitate understanding of the present invention.

[0020] The present invention provides a hair loss prevention and growth liquid containing collagen carbon quantum dots, comprising the following raw materials in weight percentage: 0.0001-0.1% collagen carbon quantum dots, 1-40% nutritional repair agent, 0.1-10% penetration enhancer, 0.01-2% protective agent, an appropriate amount of pH adjuster, and purified water to 100%. Preferably, the nutritional repair agent is one or more of collagen, sodium hyaluronate, vitamin B, vitamin C, vitamin E, arginine, lysine, ceramide, caffeine, tea polyphenols, ubiquinone, menthol, plant extract, stem cell extract, and exosomes. The penetration enhancer is one or more of oleic acid, linoleic acid, ethyl oleate, isopropyl myristate, menthol, limonene or propylene glycol, glycerin, polyethylene glycol, laurocaprol, polysorbate-80, DMSO, salicylic acid, and ethanol. The protective agent is one or more of vitamin E, tea polyphenols, ascorbic acid, cyclodextrin, glycerin, EDTA, phenoxyethanol, polysorbate 20, chlorphenesin, and trehalose. The pH adjuster is one or more of sodium hydroxide, sodium bicarbonate, triethanolamine, acetic acid, lactic acid, sodium citrate, glycine, arginine, salicylic acid, fruit acid, phosphoric acid, and boric acid.

[0021] The present invention also provides a method for preparing the above-mentioned anti-hair loss and hair growth liquid containing collagen carbon quantum dots, the flow chart of which is as follows: Figure 1 As shown, the following steps are included: S1: Collagen sponge is carbonized, purified, and freeze-dried to prepare collagen carbon quantum dot powder, wherein the particle size of the collagen carbon quantum dot powder is 2-20 nm. The specific steps of S1 are: SS1: Weigh 0.1-50 g of collagen sponge into a crucible and heat it in a box furnace at 200-300°C for 0.5-5 h; SS2: Return the SS1 sample to room temperature, add 1-100 g of purified water, grind and stir for 0.5-3 h, collect the mixture, centrifuge at 5000-30000 × g for 10-60 min, and collect the supernatant; SS3: Filter the supernatant collected from SS2 through a 5-20 μm filter membrane, then through a 0.22 μm filter membrane. Transfer the collected filtrate into a 100-5000 Da dialysis bag and dialyze with magnetic stirring for 12-72 h. Change the water 3-5 times during the purification process. Collect the solution in the bag after the dialysis is completed. SS4: The solution obtained in SS3 was freeze-dried / low-temperature dried at a temperature of -40-40°C and a vacuum degree of 10-100 Pa for 10-120 h to obtain collagen carbon quantum dot powder.

[0022] S2: Weigh the nutritional repair agent, permeation enhancer, and protective agent in proportion, add purified water and stir to mix, homogenize for 0.5-5 hours, and then adjust the pH to 5.0-6.5; S3: Add the collagen carbon quantum dot powder prepared in S1, mix homogenously for 0.5-2 h, and then filter with a 0.22 μm filter membrane. Collect the filtered sterile solution, use a filling machine to package the liquid in a packaging bottle, and use it by spraying or applying. Example 1

[0023] A hair loss prevention and growth liquid containing collagen carbon quantum dots comprises the following raw materials in percentage by weight: 0.001% collagen carbon quantum dots, 0.75% collagen, 0.1% menthol, 1% glycerol, 0.1% vitamin E, and the balance made up of purified water.

[0024] S1: Preparation of collagen carbon quantum dots: Weigh 2 g of collagen sponge in a crucible and heat it in a box furnace at 250°C for 2 h. After the sample returns to room temperature, add 50 g of purified water and grind and stir for 2 h. Collect the mixed solution and centrifuge it at 15,000 × g for 15 min. Collect the supernatant and filter it with a 10 μm filter membrane, then filter it with a 0.22 μm filter membrane. Transfer the filtrate to a 500 Da dialysis bag and dialyze it in purified water with magnetic stirring for 48 h, changing the water 3 times during the period. After the dialysis, collect the solution in the bag and freeze-dry it at a temperature of -30°C and a vacuum degree of 50 Pa for 72 h to collect the powdered collagen carbon quantum dots. Take the collagen carbon quantum dot powder and dissolve it in purified water at a concentration of 0.01%. Figure 2 As shown, collagen carbon quantum dots exhibit fluorescence properties under ultraviolet light.

[0025] S2: Weigh 0.75 g collagen, 0.1 g menthol, 1 g glycerol, and 0.1 g vitamin E, add purified water to make up to 100 g, stir and mix, homogenize for 2 h, add disodium hydrogen phosphate / sodium dihydrogen phosphate to adjust the pH to 6.0, and stir and mix.

[0026] S3: Weigh 1 mg of collagen-derived carbon quantum dots and add them to the solution. After homogenizing for 1 hour, filter the solution through a 0.22 μm filter membrane and collect the collected solution. Use a filling machine to package the solution into a bottle. After assembling the nozzle, pump head, and dust cap, the anti-hair loss and hair growth solution is obtained. Direct the nozzle directly toward the scalp and spray. Example 2

[0027] Disclosed is an anti-hair loss and hair growth liquid containing collagen carbon quantum dots, comprising the following raw materials in percentage by weight: 0.02% collagen carbon quantum dots, 0.5% sodium hyaluronate, 0.05% caffeine, 0.5% linoleic acid, 0.4% salicylic acid, 0.5% trehalose, and the balance made up of purified water.

[0028] S1: Preparation of Collagen Carbon Quantum Dots: Weigh 20 g of collagen sponge into a crucible and heat at 280°C in a box furnace for 3 h. After the sample returned to room temperature, add 200 g of purified water and grind with stirring for 3 h. The mixture was collected and centrifuged at 20,000 × g for 30 min. The supernatant was collected and filtered through a 20 μm filter membrane, then through a 0.22 μm filter membrane. The filtrate was transferred to a 500 Da dialysis bag and dialyzed in purified water with magnetic stirring for 72 h, changing the water five times. After dialysis, the solution in the bag was dried at 15°C and 50 Pa vacuum for 60 h to obtain powdered collagen carbon quantum dots.

[0029] S2: Weigh 1 g sodium hyaluronate, 0.1 g caffeine, 1 g linoleic acid, 0.8 g salicylic acid, and 1 g trehalose, add purified water to make up to 200 g, stir and mix, homogenize for 2.5 h, add citric acid to adjust the pH to 5.5, and stir and mix.

[0030] S3: Weigh 40 mg of collagen-derived carbon quantum dots and add them to the solution. Homogenize for 2 hours, then filter the solution through a 0.22 μm filter membrane and collect the remaining solution. Use a filling machine to package the solution in a bottle, assemble the bottle cap, and obtain the anti-hair loss and hair growth solution. Squeeze the solution onto your palm and apply to the skin. Example 3

[0031] A hair loss prevention and growth liquid containing collagen carbon quantum dots comprises the following raw materials in percentage by weight: 0.005% collagen carbon quantum dots, 0.2% ceramide, 3% propylene glycol, 2% polyethylene glycol, 0.2% ascorbic acid, and the balance made up of purified water.

[0032] S1: Preparation of Collagen Carbon Quantum Dots: Weigh 5 g of collagen sponge into a crucible and heat at 280°C in a box furnace for 1.5 h. After the sample returned to room temperature, add 100 g of purified water and grind with stirring for 1.5 h. The mixture was collected and centrifuged at 30,000 × g for 10 min. The supernatant was collected and filtered through a 15 μm filter membrane, then through a 0.22 μm filter membrane. The filtrate was transferred to a 500 Da dialysis bag and dialyzed in purified water with magnetic stirring for 36 h, changing the water five times. After dialysis, the solution in the bag was dried at 0°C and 50 Pa vacuum for 45 h to obtain powdered collagen carbon quantum dots.

[0033] S2: Weigh 0.6 g ceramide, 9 g propylene glycol, 6 g polyethylene glycol, and 0.6 g ascorbic acid, add purified water to make up to 300 g, stir and mix, emulsify and homogenize for 3.5 h, add citric acid to adjust the pH to 5.5, and stir and mix.

[0034] S3: Weigh 15 mg of collagen-derived carbon quantum dots and add them to the solution. After homogenizing for 2.5 hours, filter the solution through a 0.22 μm filter membrane and collect the collected solution. Use a filling machine to package the solution in a bottle and assemble the cap to obtain the anti-hair loss and hair growth solution. Squeeze the liquid onto the palm of your hand and apply it to the affected area.

[0035] Comparative Example 1 (without collagen carbon quantum dots) Compared with Example 1, the difference of Comparative Example 1 is that: in Comparative Example 1, step S1 is removed, and the collagen carbon quantum dots in step S3 are replaced with an equal weight portion of purified water, and the other steps remain unchanged to prepare a mixed hair growth agent.

[0036] Comparative Example 2 (without nutritional repair agent) Compared with Example 1, the difference of Comparative Example 2 is that: in Comparative Example 2, the nutritional repair agent collagen in step S2 is replaced with an equal weight of purified water, and the other steps remain unchanged to prepare a mixed hair growth agent.

[0037] Comparative Example 3 (without penetration enhancer) Compared with Example 1, Comparative Example 3 is different in that: in Comparative Example 3, the penetration enhancers menthol and glycerin in step S2 are replaced with purified water in equal parts by weight, and the remaining steps remain unchanged to prepare a mixed hair growth agent.

[0038] Cell proliferation assay: Human hair follicle dermal papilla cells (HFDPC) were cultured at 7000 cells / cm 2Cells were seeded at a density of 100 μg / mL in 24-well plates and divided into seven groups: a control group (culture medium) and sample groups (Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3), with three replicates per group. After 6 hours of adherence, the cells were starved for 12 hours using serum-free culture medium. The culture medium was then replaced with 90% serum-free culture medium supplemented with 10% ultrapure water for the control group and 90% serum-free culture medium supplemented with 10% hair growth solution for the sample group. After 48 hours of culture, cell viability was assessed using CCK-8, and the proliferation rate was calculated. The experimental results are shown in Table 1. Cell-based experiments demonstrated that the addition of collagen carbon quantum dots and a nutrient-repairing agent significantly promoted the proliferation of human hair follicle dermal papilla cells, thereby activating the hair follicle growth cycle and delaying hair follicle degeneration.

[0039] Table 1 Statistics of HFDPC cell proliferation rate

[0040] Animal experiments: Twenty-eight SD rats, 6 to 8 weeks old, half male and half female, with intact hair, were shaved from the mid-dorsal area (2 × 2 cm²). Dihydrotestosterone (DHT) was then applied once daily to induce an androgenic alopecia (AGA) model. Four to six weeks after modeling, the rats were randomly divided into seven groups: a control group and sample groups (Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3), with four rats in each group. Hair follicles were counted in three random visual fields. The results are shown in Table 2.

[0041] The control group applied 1 mL of purified water, and the sample groups applied 1 mL of the corresponding hair growth solution, once daily, morning and evening. After four weeks of the experiment, the hair growth of the seven groups of experimental animals was observed, and the number of hair follicles in the field of view was counted using a trichoscope. The results are shown in Table 2. Based on the statistical data, the promotion rate of hair follicle growth by applying different solutions was calculated. Compared with the control group, the promotion rates of Examples 1 to 3 were all above 35%, indicating that the hair growth solution significantly promoted the growth of hair follicles in AGA rats. Comparative Example 1 did not contain collagen carbon quantum dots and had no significant promoting effect, indicating that carbon quantum dots are the main active ingredient in the hair growth solution that promotes hair follicle growth. The promotion rate of Comparative Example 2 was 17.24%, indicating that the addition of the nutritional repair agent can promote hair follicle growth to a certain extent. The promotion rate of Comparative Example 3 was 34.48%, indicating that the penetration enhancer does not promote hair follicle growth, but rather has a beneficial effect by promoting the penetration of the active ingredient.

[0042] Table 2 Statistics of hair follicle growth in experimental animals

[0043] This document uses specific examples to illustrate the inventive concept in detail. The above embodiments are only intended to help understand the core concept of the present invention. It should be noted that any obvious modifications, equivalent substitutions, or other improvements made by a person skilled in the art without departing from the inventive concept should be included within the scope of protection of the present invention.

Claims

1. An anti-hair loss and hair growth liquid containing collagen carbon quantum dots, characterized in that: The invention comprises the following raw materials in percentage by weight: 0.0001-0.1% of collagen carbon quantum dots, 1-40% of nutritional repair agent, 0.1-10% of penetration enhancer, 0.01-2% of protective agent, an appropriate amount of pH regulator, and purified water to 100%.

2. The anti-hair loss and hair growth liquid containing collagen carbon quantum dots according to claim 1, characterized in that: The nutritional repair agent is one or more of collagen, sodium hyaluronate, vitamin B, vitamin C, vitamin E, arginine, lysine, ceramide, caffeine, tea polyphenols, ubiquinone, menthol, plant extract, stem cell extract, and exosomes.

3. The anti-hair loss and hair growth liquid containing collagen carbon quantum dots according to claim 2, characterized in that: The penetration enhancer is one or more of oleic acid, linoleic acid, ethyl oleate, isopropyl myristate, menthol, limonene or propylene glycol, glycerol, polyethylene glycol, laurocaprol, polysorbate 80, DMSO, salicylic acid or ethanol.

4. The anti-hair loss and hair growth liquid containing collagen carbon quantum dots according to claim 3, characterized in that: The protective agent is one or more of vitamin E, tea polyphenols, ascorbic acid, cyclodextrin, glycerol, EDTA, phenoxyethanol, polysorbate 20, chlorphenesin, and trehalose.

5. The anti-hair loss and hair growth liquid containing collagen carbon quantum dots according to claim 4, characterized in that: The pH regulator is one or more of sodium hydroxide, sodium bicarbonate, triethanolamine, acetic acid, lactic acid, sodium citrate, glycine, arginine, salicylic acid, fruit acid, phosphoric acid, and boric acid.

6. A method for preparing the anti-hair loss and hair growth liquid containing collagen carbon quantum dots according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1: Carbonizing, purifying, and freeze-drying a collagen sponge to obtain collagen carbon quantum dot powder, wherein the particle size of the collagen carbon quantum dot powder is 2-20 nm; S2: Weigh the nutritional repair agent, permeation enhancer, and protective agent in proportion, add purified water and stir to mix, homogenize and mix for 0.5-5 hours, then adjust the pH to 5.0-6.5; S3: Add the collagen carbon quantum dot powder prepared in S1, mix homogenously for 0.5-2 h, and then filter with a 0.22 μm filter membrane. Collect the filtered sterile solution, use a filling machine to package the liquid in a packaging bottle, and use it by spraying or applying.

7. The method for preparing the anti-hair loss and hair growth liquid containing collagen carbon quantum dots according to claim 6, characterized in that: The specific steps of S1 are: SS1: Weigh 0.1-50 g of collagen sponge into a crucible and heat it in a box furnace at 200-300°C for 0.5-5 h; SS2: Return the SS1 sample to room temperature, add 1-100 g of purified water, grind and stir for 0.5-3 h, collect the mixture, centrifuge at 5000-30000 × g for 10-60 min, and collect the supernatant; SS3: Filter the supernatant collected from SS2 through a 5-20 μm filter membrane, then through a 0.22 μm filter membrane. Transfer the collected filtrate into a 100-5000 Da dialysis bag and dialyze in purified water with magnetic stirring for 12-72 h, changing the water 3-5 times during the process. Collect the solution in the bag after the dialysis is completed. SS4: The solution obtained in SS3 was freeze-dried / low-temperature dried at a temperature of -40-40°C and a vacuum degree of 10-100 Pa for 10-120 hours to obtain collagen carbon quantum dot powder.

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