Kit for promoting hair growth

Abstract

Disclosed herein is a kit for promoting hair growth and preventing hair loss, comprising (i) a first composition; (ii) a second composition; (iii) a third composition; (iv)a fourth composition and (v) an instruction manual. Disclosed herein is also is a method of using the kit for promoting hair growth and preventing hair loss.

Description

[0001]5 “KIT FOR PROMOTING HAIR GROWTH” TECHNICAL FIELD OF THE INVENTION: The present invention relates to a kit for promoting hair growth. More 10 particularly, the present invention relates to a kit for promoting hair growth and preventing hair loss, comprising (i) a first composition; (ii) a second composition; (iii) a third composition; (iv) a fourth composition; and (v) an instruction manual. The present invention further relates to a method of using the kit for promoting hair growth and preventing hair loss. 15 BACKGROUND AND PRIOR ART: Men and women around the world suffer from hair thinning and hair loss (alopecia). It is one of the major causes of concern for people all around the world. Not only does it affect a person's psychological well-being, it also lowers 20 their self-confidence. Hair loss can be either temporary or permanent and can affect just the scalp or the whole body. There are several types of hair, including terminal hair, vellus hair, and deformed terminal hair such as eyebrows and eyelashes. The most common types of hair 25 loss include male or female pattern baldness, alopecia areata, and telogen effluvium. As alopecia progresses, a transition occurs in areas approaching baldness, during which the hair itself changes from terminal to vellus type. It is known to reduce the size of hair follicles. 30 Hair loss can be caused by a variety of factors such as decreased hair root metabolic function, decreased hair function, reduced blood circulation, Alopecia Areata (AA), hormonal imbalance, drug side effects, stress, genetic factors and various nutritional deficiencies. Certain medications including chemotherapy, HIV / AIDS and hypothyroidism can also cause hair loss. Hair loss may also be 5 induced due to unhealthy lifestyle, heat or chemical hair treatments or textural inflictions to hair due to aggressive and repeated hair coloring and hair styling. Excessive styling and hair treatments can also cause hair loss. Examples of extreme styling include tight braids, hair weaves, cornrows, chemical relaxers to 10 straighten hair, hot oil treatments, or any kind of harsh chemical or high heat. Such practices affect the hair root, so hair may not grow back. Frequent shampooing, blow-drying and styling of hair causes a lot of breakage and damage to hair, which may lead to hair loss and hair thinning. Repeated use of 15 heated styling tools, pulling of hair (such as during blow-drying or making a tight ponytail), and rubbing the scalp too hard can all lead to hair loss. Perms, relaxers, and hair dyes can also cause hair loss due to damage. Women suffer from hormone-related hair loss, or female pattern baldness. This 20 hair loss occurs when the hair follicle shrinks over time too much to regrow new hair. Symptoms of female pattern baldness may include hair spreading in the middle and coarsening on the face. In many cases, this type of hair loss can be genetic. 25 Medicinal treatments for alopecia include the drugs like Rogaine (minoxidil) and Propecia (finasteride). Rogaine has been shown to reduce hair loss and promote hair growth in up to 10% of men with male pattern baldness. However, treatment with it needs to be done regularly and has many side effects. The treatment is also very expensive. Finasteride is the active ingredient in Propecia (a trademark of 30 Merck & Co.) in tablet form and should be taken regularly. Alopecia areata can be treated with steroid injections into the affected area, but these must be repeated frequently to be effective. Other treatments include Surgical techniques such as Self-grafting or transplants, Flap surgery, Scalp 35 reductions and Scalp expansion and extension and Non- surgical techniques such 5 as additions of hair- bearing devices that can consist of human hair, synthetic fibre or a combination of both. These attachments are attached by a variety of techniques, and either existing hair or skin can be the fixation site. US5750108 discloses a method for hair treatment and a kit for invigorating hair 10 growth. The hair treatment kit comprises a first treatment solution comprising tea tree oil, a second treatment solution comprising chlorine dioxide, an acidic solution having a pH effective to release the oxygen from said chlorine dioxide in said second treatment solution, and a third treatment solution comprising saw palmetto berry extract. 15 WO2005004984A1 discloses a kit, device and a method for the treatment of a hair growth disorder, wherein the kit comprises: at least one formulation comprising a hair growth modulating agent; and at least one electrically powered device for promoting delivery of the hair growth modulating agent into and / or onto the body 20 area. US8197856 discloses kit for promoting hair growth on areas of the scalp that has been affected by fungal infections. The kit comprises of a first powdered mixture and three liquid compositions. The elements of the kit are applied to the scalp of 25 an individual experiencing hair loss due to fungi. The hair growth kits available in existing prior arts are expensive and have shown unsatisfactory results. The results also are short-lived and carry the risk of unwanted side effects. 30 Therefore, there is a need in the art to provide a kit for promoting hair growth that can improve hair growth, prevent hair loss, strengthen hair follicles and hydrate the hair and which is economical and easy to apply. 5 Hence, the present inventors have come up with a kit for prevention of early hair loss and promotion of hair growth. OBJECT OF INVENTION: 10 It is therefore an object of the present invention to provide a kit for promotion of hair growth. Another object of the invention is to provide a kit for promotion of hair growth, prevention of hair loss and further strengthening and nourishment of hair. The said 15 kit comprises constituents which are cost effective, non-toxic, biocompatible and hence favor prolonged use with minimal to no side effects. It is yet another object of the present invention to provide a method of using the said kit for promoting hair growth and preventing hair loss. 20 SUMMARY OF THE INVENTION: In an aspect, the present invention provides a kit for promoting hair growth and preventing hair loss, comprising: i. a first composition consisting of Vascular Endothelial Growth Factor25 (Human oligopeptide-11) including its biomimetic analogue Sh- Polypeptide-9, Basic Fibroblast Growth Factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1, Insulin-like Growth Factor 1 (Human oligopeptide-2) including its biomimetic analogue Sh- Oligopeptide-2, Copper Tripeptide 1, Keratinocyte Growth Factor 30 including its biomimetic analogue Sh-Polypeptide-3, Thymosin-β4 including its biomimetic analogue Sh-Oligopeptide-4; ii. a second composition consisting of Anagain, Caffeine, Vitamin B5, Vitamin E (Tocopheryl Acetate) and Citric Acid; together with pharmaceutically acceptable excipients and additives in a suitable vehicle; 5 iii. a third composition consisting of Niacinamide, Onion Oil, Procapil, Anagain, Redensyl, Saw Palmetto Oil, Biotin and Melatonin; together with pharmaceutically acceptable excipients and additives in a suitable vehicle; iv. a fourth composition consisting of nutraceuticals together with pharmaceutically acceptable excipients; and 10 v. an instruction manual. In a further aspect, the first composition comprises; i. Vascular endothelial growth factor ( Human oligopeptide-11) including its biomimetic analogue Sh-Polypeptide-9 in an amount of 0.01mg / L – 15 100mg / L; ii. Basic fibroblast growth factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1 in an amount of 0.01mg / L – 100 mg / L; iii. Insulin like growth factor (Human oligopeptide-2) including its 20 biomimetic analogue Sh-Oligopeptide-2 in an amount of 0.01mg / L – 100mg / L; iv. Copper tripeptide 1 in an amount of 0.1mg / L – 500 mg / L; v. Keratinocyte growth factor including its biomimetic analogue Sh- Polypeptide-3 in an amount of 0.01mg / L – 100 mg / L; and 25 vi. Thymosin β4 including its biomimetic analogue Sh-Oligopeptide-4 in an amount of 0.005mg / L – 100 mg / L. In another aspect, the second composition comprises: i. Anagain in an amount of 0.5 - 3.0% w / w; 30 ii. Caffeine in an amount of 0.5 - 3.0% w / w; iii. Vitamin B5 in an amount of 0.2 – 1.0 % w / w; iv. Vitamin E in an amount of 0.01 – 0.5 % w / w; and v. Citric acid in an amount of 0.005 – 0.05 % w / w together with pharmaceutically acceptable excipients and additives in a 35 suitable vehicle. In yet another aspect, the pharmaceutically acceptable excipients of the second composition comprises: i. Polyquaternium 10 in an amount of 0.5 - 3.0% w / w; ii. Glycerin in an amount of 2.0 – 7.0 % w / w; 10 iii. Sodium lauryl ether sulphate in an amount of 30 – 60% w / w; iv. Sodium lauroyl sarcosinate in an amount of 1.0 – 3.0% w / w; v. Propylene glycol in an amount of 0.5 - 3.0% w / w; vi. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.01 – 0.5 % w / w; 15 vii. Biosaccharide Gum-1 in an amount of 0.2 – 1.0 % w / w; viii. Sodium chloride in an amount of 0.2 – 1.0 % w / w; ix. Cyclopentasiloxane and Dimethiconol in an amount of 0.2 – 1.0 % w / w; x. Perfume in an amount of 0.5 - 3.0% w / w; xi. Pearl powder in an amount of 0.01 – 0.5 % w / w; 20 xii. White powder in an amount of 0.01 – 0.5 % w / w; xiii. Disodium EDTA in an amount of 0.01 – 0.5 % w / w; and xiv. Aqua- Q.S In another aspect, the additives of the second composition comprises: i. Kappaphycus alvarezii extract in an amount of 0.1 – 1.0 % w / w; 25 ii. Vaccinium macrocarpon fruit extract (Cranberry extract) in an amount of 0.1 – 1.0 % w / w; iii. Dextran Hydroxypropyltrimonium Chloride in an amount of 1.0 – 10 % w / w; and iv. Saw palmetto extract in an amount of 0.005 – 5 % w / w. 30 In a further aspect, the third composition comprises: i. Niacinamide in an amount of 0.2 – 1.0 % w / w; ii. Onion Oil in an amount of 0.2 – 1.0 % w / w; iii. Procapil in an amount of 0.5 - 3.0% w / w; 35 iv. Anagain in an amount of 0.2 – 1.0 % w / w; 5 v. Redensyl in an amount of 0.05 – 0.5% w / w; vi. Saw Palmetto Oil in an amount of 0.2 – 1.0 % w / w; vii. Biotin in an amount of 0.01 – 0.5 % w / w; and viii. Melatonin in an amount of 0.005 – 0.05 % w / w; together with pharmaceutically acceptable excipients and additives in a suitable 10 vehicle. In yet another aspect, the pharmaceutically acceptable excipients of the third composition comprises: i. Glycerin in an amount of 2.0 – 7.0 % w / w; 15 ii. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.5 – 3.0 % w / w; iii. Biosaccharide Gum-1 in an amount of 0.5 – 3.0 % w / w; iv. Sodium Metabisulfite in an amount of 0.2 – 1.0 % w / w; v. Hydroxyethyl Cellulose in an amount of 0.2 – 1.0 % w / w; vi. Xanthan Gum in an amount of 0.2 – 1.0 % w / w; 20 vii. Perfume in an amount of 0.2 – 1.0 % w / w; viii. Benzyl Alcohol in an amount of 0.2 – 1.0 % w / w; ix. Triethanolamine in an amount of 0.1 – 0.5% w / w; x. Disodium EDTA in an amount of 0.01 – 0.5 % w / w; and xi. Aqua– Q.S. 25 In another aspect, the additives of the third composition comprises: i. Capixyl in an amount of 1.0 – 10 % w / w; ii. Aminexil in an amount of 0.5 – 5.0 % w / w; iii. Caffeine in an amount of 0.001 – 0.01 % w / w; 30 iv. Caprylic / capric triglyceride in an amount of 0.5 – 5.0 % w / w; v. Ginseng extract in an amount of 1.0 – 10 % w / w; and vi. Ginkgo biloba extract in an amount of 1.0 – 10 % w / w. 5 In an aspect, the fourth composition comprises nutraceuticals selected from the group comprising of vitamins, metal salts, plant extracts, amino acids, pigments, probiotics, Omega fatty acids and the like. In another aspect, the fourth composition comprises: 10 i. Vitamins in an amount of 1-2 % w / w; ii. Metal salts in an amount of 0.2-0.5 % w / w; iii. Plant extracts in an amount of 5-8 % w / w; iv. Amino acids in an amount of 1-2 % w / w; v. Pigments in an amount of 0.2-0.5 % w / w; 15 vi. Probiotics in an amount of 0.1-0.2 % w / w; and vii. Omega fatty acids in an amount of 0.4-0.6 % w / w. The hair growth promoting kit of the present invention is useful for prevention of hair loss by stimulating hair follicles and promoting hair growth and strengthening and nourishing hair to protect the hair and enhance the overall health of the hair. 20 DETAILED DESCRIPTION OF THE INVENTION: In line with the object of present invention, there is provided a kit for promoting hair growth, preventing hair loss and nourishing of hair follicles in mammals. 25 As used herein the term ‘effective amount’ means an amount which stimulates hair growth associated with hair loss afflictions like male pattern baldness, or insults such as radiation or chemotherapy. Source and Geographical Origin: Material Source Geographical Origin Pisum Sativum Sprout South Korea Mediterranean Basin and the Near East Extract Larix Europaea Wood South Korea Mountains of Central Europe, in the Extract Alps and Carpathian Mountains and the Pyrenees Camellia Sinensis Leaf South Korea East Asia, probably originated in the Extract borderlands of north Burma and southwestern China Centella Asiatica leaf South Korea Tropical regions of Africa, Asia, extract Australia, and islands in the western Pacific Ocean Cranberry fruit dried South Korea Fen (marsh) lands of England extract Holy basil South Korea Indian subcontinent Fenugreek seed extract South Korea South Eastern Europe and West Asia Ginkgo biloba South Korea China Grape seed extract South Korea Central Asia Ashwagandha root South Korea India, northern Africa, and the Middle extract East Pumpkin seed extract South Korea Northeastern Mexico and Texas Brahmi leaf extract South Korea Southern and Eastern India, Australia, Europe, Africa, Asia, and North and South America Cinnamon bark extract South Korea Sri Lanka (formerly Ceylon), the neighbouring Malabar Coast of India, and Myanmar (Burma) Coconut powder South Korea Coastal areas of Southeast Asia and Melanesia Ginger rhizome extract South Korea Southeastern Asia Glycyrrhiza glabra South Korea Eurasia, in central and south-western extract Asia and the Mediterranean region Rose mary leaves South Korea Mediterranean region extract Ginseng extract South Korea Korean Peninsula, Northeast China, Russian Far East, Canada and the United State Kappaphycus alvarezii South Korea Indo-Pacific region Extract Vaccinium macrocarpon South Korea from the East Coast to the Central U. S. and Canada and from Southern Canada in the north to the Appalachians in the south Saw palmetto extract South Korea south-eastern United States 5 Accordingly, the present invention provides a kit for promoting hair growth and preventing hair loss, wherein the kit contains compositions comprising active ingredients together with pharmaceutically acceptable excipients that are mild, eco-friendly and prolong the anagen phase of hair cycle and prevent hair loss. 10 Further, the kit of the present invention promotes hair growth, strengthens the hair follicles and hydrates the hair from the scalp to the tips. The present hair growth promoting kit avoids the use of costly and harmful materials commonly used in hair growth kits of the art. 15 In a main embodiment, the present invention provides a kit for promoting hair growth and preventing hair loss, comprising: (i) a first composition (ii) a second composition, (iii) a third composition (iv) a fourth composition, and (v) an instruction manual. 20 In an embodiment, the present invention provides a kit for promoting hair growth and preventing hair loss, comprising: i. a first composition consisting of Vascular Endothelial Growth Factor (Human oligopeptide-11) including its biomimetic analogue Sh- Polypeptide-9, Basic Fibroblast Growth Factor (Human oligopeptide-3) 25 including its biomimetic analogue Sh-Polypeptide-1, Insulin-like Growth Factor 1 (Human oligopeptide-2) including its biomimetic analogue Sh- 5 Oligopeptide-2, Copper Tripeptide 1, Keratinocyte Growth Factor including its biomimetic analogue Sh-Polypeptide-3, Thymosin-β4 including its biomimetic analogue Sh-Oligopeptide-4; ii. a second composition consisting of Anagain, Caffeine, Vitamin B5, Vitamin E and Citric acid; together with pharmaceutically acceptable 10 excipients and additives in a suitable vehicle; iii. a third composition consisting of Niacinamide, Onion Oil, Procapil, Anagain, Redensyl, Saw Palmetto Oil, Biotin and Melatonin; together with pharmaceutically acceptable excipients and additives in a suitable vehicle; 15 iv. a fourth composition consisting of nutraceuticals together with pharmaceutically acceptable excipients; and v. an instruction manual. In another embodiment, the first composition comprises; 20 i. Vascular endothelial growth factor ( Human oligopeptide-11) including its biomimetic analogue Sh-Polypeptide-9 in an amount of 0.01mg / L – 100mg / L; ii. Basic fibroblast growth factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1 in an amount of 0.01mg / L – 100 25 mg / L; iii. Insulin like growth factor (Human oligopeptide-2) including its biomimetic analogue Sh-Oligopeptide-2 in an amount of 0.01mg / L – 100mg / L; iv. Copper tripeptide 1 in an amount of 0.1mg / L – 500 mg / L; 30 v. Keratinocyte growth factor including its biomimetic analogue Sh- Polypeptide-3 in an amount of 0.01mg / L – 100 mg / L; and vi. Thymosin β4 including its biomimetic analogue Sh-Oligopeptide-4 in an amount of 0.005mg / L – 100 mg / L. In yet another embodiment, the second composition comprises: 35 i. Anagain in an amount of 0.5 - 3.0% w / w; 5 ii. Caffeine in an amount of 0.5 - 3.0% w / w; iii. Vitamin B5 in an amount of 0.2 – 1.0 % w / w; iv. Vitamin E in an amount of 0.01 – 0.5 % w / w; and v. Citric acid in an amount of 0.005 – 0.05 % w / w; together with pharmaceutically acceptable excipients and additives in a 10 suitable vehicle. In another embodiment, the pharmaceutically acceptable excipients of the second composition comprises: i. Polyquaternium 10 in an amount of 0.5 - 3.0% w / w; 15 ii. Glycerin in an amount of 2.0 – 7.0 % w / w; iii. Sodium lauryl ether sulphate in an amount of 30 – 60% w / w; iv. Sodium lauroyl sarcosinate in an amount of 1.0 – 3.0% w / w; v. Propylene glycol in an amount of 0.5 - 3.0% w / w; vi. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.01 – 0.5 % 20 w / w; vii. Biosaccharide Gum-1 in an amount of 0.2 – 1.0 % w / w; viii. Sodium chloride in an amount of 0.2 – 1.0 % w / w; ix. Cyclopentasiloxane and Dimethiconol in an amount of 0.2 – 1.0 % w / w; x. Perfume in an amount of 0.5 - 3.0% w / w; 25 xi. Pearl powder in an amount of 0.01 – 0.5 % w / w; xii. White powder in an amount of 0.01 – 0.5 % w / w; xiii. Disodium EDTA in an amount of 0.01 – 0.5 % w / w; and xiv. Aqua- Q.S In another embodiment, the active ingredient Anagain comprises of Pisum 30 sativam sprout extract and Phenoxyethanol and Aqua. In another embodiment, the additives of the second composition comprises: i. Kappaphycus alvarezii Extract in an amount of 0.1 – 1.0 % w / w; ii. Vaccinium macrocarpon Fruit Extract (Cranberry extract) in an amount of 35 0.1 – 1.0 % w / w; 5 iii. Dextran Hydroxypropyltrimonium Chloride in an amount of 1.0 – 10 % w / w; and iv. Saw palmetto extract in an amount of 0.005 – 5 % w / w. The second composition of the present invention helps in arresting hair fall and 10 increasing the thickness and density of hair. It contains natural ingredients which help to activate hair follicles from the dormant phase and results in long, shiny and bouncy hair. It also helps in conditioning the hair. In an embodiment, the third composition of the kit comprises Niacinamide, Onion 15 Oil, Procapil, Anagain, Redensyl, Saw palmetto extract, Biotin and Melatonin as active ingredients, together with pharmaceutically acceptable excipients and additives in a suitable vehicle. In a further embodiment, the third composition comprises: 20 i. Niacinamide in an amount of 0.2 – 1.0 % w / w; ii. Onion Oil in an amount of 0.2 – 1.0 % w / w; iii. Procapil in an amount of 0.5 - 3.0% w / w; iv. Anagain in an amount of 0.2 – 1.0 % w / w; v. Redensyl in an amount of 0.05 – 0.5% w / w; 25 vi. Saw Palmetto Oil in an amount of 0.2 – 1.0 % w / w; vii. Biotin in an amount of 0.01 – 0.5 % w / w; and viii. Melatonin in an amount of 0.005 – 0.05 % w / w; together with pharmaceutically acceptable excipients and additives in a suitable vehicle. 30 In another embodiment, the active ingredient Anagain comprises of Pisum sativum (Pea) Sprout Extract, Alcohol and Aqua / Water. 5 In yet another embodiment, the active ingredient Redensyl comprises of Glycerin, Sodium Metabisulfite, Larix Europaea Wood Extract, Glycine, Zinc Chloride, Camellia Sinensis Leaf Extract and Aqua. In another embodiment, the active ingredient Procapil comprises of Butylene 10 Glycol, PPG-26-Buteth-26, PEG-40 Hydrogenated Castor Oil, Apigenin, Oleanolic Acid, Biotinoyl Tripeptide-1 and Aqua. In an embodiment, the pharmaceutically acceptable excipients of the third composition include but not limited to Glycerin, Phenoxyethanol and 15 Ethylhexylglycerin, Biosaccharide Gum-1, Sodium Metabisulfite, Hydroxyethyl Cellulose, Xanthan Gum, Perfume, Benzyl Alcohol, Triethanolamine, Disodium EDTA and Aqua. In yet another embodiment, the pharmaceutically acceptable excipients of the 20 third composition comprises: i. Glycerin in an amount of 2.0 – 7.0 % w / w; ii. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.5 – 3.0 % w / w; iii. Biosaccharide Gum-1 in an amount of 0.5 – 3.0 % w / w; iv. Sodium Metabisulfite in an amount of 0.2 – 1.0 % w / w; 25 v. Hydroxyethyl Cellulose in an amount of 0.2 – 1.0 % w / w; vi. Xanthan Gum in an amount of 0.2 – 1.0 % w / w; vii. Perfume in an amount of 0.2 – 1.0 % w / w; viii. Benzyl Alcohol in an amount of 0.2 – 1.0 % w / w; ix. Triethanolamine in an amount of 0.1 – 0.5% w / w; 30 x. Disodium EDTA in an amount of 0.01 – 0.5 % w / w; and xi. Aqua– Q.S In another embodiment, the additives of the third composition comprises: i. Capixyl in an amount of 1.0 – 10 % w / w; 35 ii. Aminexil in an amount of 0.5 – 5.0 % w / w; 5 iii. Caffeine in an amount of 0.001 – 0.01 % w / w; iv. Caprylic / capric triglyceride in an amount of 0.5 – 5.0 % w / w; v. Ginseng extract in an amount of 1.0 – 10 % w / w; and vi. Ginkgo biloba extract in an amount of 1.0 – 10 % w / w. 10 In an embodiment, the fourth composition comprises nutraceuticals including but not limited to vitamins, metal salts, plant extracts, amino acids, pigments, probiotics, Omega fatty acids and the like. In another embodiment, the fourth composition comprises: 15 i. Vitamins in an amount of 1-2 % w / w; ii. Metal salts in an amount of 0.2-0.5 % w / w; iii. Plant extracts in an amount of 5-8 % w / w; iv. Amino acids in an amount of 1-2 % w / w; v. Pigments in an amount of 0.2-0.5 % w / w; 20 vi. Probiotics in an amount of 0.1-0.2 % w / w; and vii. Omega fatty acids in an amount of 0.4-0.6 % w / w; In yet another embodiment, the vitamins of the fourth composition are selected from the group consisting of Vitamin B1, Vitamin B2, Vitamin B3, Vitamin B5, Vitamin B6, Vitamin B7, Vitamin B9, Vitamin B12, Vitamin C, Vitamin D2, 25 Vitamin E and the like. In another embodiment, the metal salts of the fourth composition are selected from the group consisting of Magnesium oxide, dicalcium phosphate, calcium, phosphorus, ferrous bisglycinate, zinc sulphate and the like. 30 In yet another embodiment, the plant extracts of the fourth composition are selected from the group consisting of Centella asiatica leaf extract, Cranberry fruit dried extract, Holy basil, Fenugreek seed extract, Ginkgo biloba, grape seed extract, ashwagandha root extract, pumpkin seed extract, Brahmi leaf extract, 5 Cinnamon bark extract, coconut powder, ginger rhizome extract, Glycyrrhiza glabra extract, rose mary leaves extract, Beta sitosterol and the like. In another embodiment, the amino acids of the fourth composition are selected from the group consisting of N-Acetyl-Cysteine, L-Ornithine, L-lysine HCl, L- 10 Histidine HCl and the like. In yet another embodiment, the pigments of the fourth composition are selected from the group consisting of Lutein, Chromium picolinate, lycopene, Citrus bioflavonoids, and the like. 15 In a further embodiment, the omega fatty acids of the fourth composition are selected from Omega 3 fatty acid, gamma-linoleic acid and the like. In a further embodiment, the probiotics of the fourth composition are selected 20 from Lactobacillus acidophilus and the like. The supplements present in the fourth composition protect the hair, boost hair growth and enhances the overall health of the hair. 25 In a further embodiment, present invention provides a process for the preparation of the first composition which includes mixing the components and water at a temperature of 25°C in a disperser, adjusting the pH and filtering the final product using a 500 MESH micron filter system as depicted in figure 1. 30 In a further embodiment, present invention provides a process for the preparation of the second composition which includes adding the components in the right proportions of Anagain, Caffeine, Vitamin B5, Vitamin E and Citric acid, together with pharmaceutically acceptable excipients and additives in a suitable vehicle, wherein the ingredients are added in proportion in the composition to achieve the 35 desired effect. 5 In another embodiment, present invention provides a process for the preparation of the third composition which includes adding the components in the right proportions of Niacinamide, Onion Oil, Procapil, Anagain, Redensyl, Saw Palmetto Oil, Biotin and Melatonin, together with pharmaceutically acceptable 10 excipients and additives in a suitable vehicle, wherein the ingredients are added in proportion in the composition to achieve the desired effect. In yet another embodiment, present invention provides a process for the preparation of the fourth composition which includes adding the components in 15 the right proportions of Vitamins, Metal salts, Plant extracts, Amino acids, Pigments, Probiotics and Omega fatty acids in a suitable vehicle, wherein the ingredients are added in proportion in the composition to achieve the desired effect. 20 In another embodiment, the first composition of the present invention comprises; i. Vascular endothelial growth factor (Human oligopeptide-11) including its biomimetic analogue Sh-Polypeptide-9 in an amount of 5 mg / L; ii. Basic fibroblast growth factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1 in an amount of 2 mg / L; 25 iii. Insulin like growth factor (Human oligopeptide-2) including its biomimetic analogue Sh-Oligopeptide-2 in an amount of 2 mg / L; iv. Copper tripeptide 1 in an amount of 10 mg / L; v. Keratinocyte growth factor including its biomimetic analogue Sh- Polypeptide-3 in an amount of 1 mg / L; and 30 vi. Thymosin β4 including its biomimetic analogue Sh-Oligopeptide-4 in an amount of 0.01 mg / L. In yet another embodiment, the second composition comprises: i. Anagain in an amount of 1.0% w / w; 35 ii. Caffeine in an amount of 1.0% w / w; 5 iii. Vitamin B5 in an amount of 0.5% w / w; iv. Vitamin E in an amount of 0.1% w / w; and v. Citric acid in an amount of 0.01% w / w; together with pharmaceutically acceptable excipients and additives in a suitable vehicle. 10 In another embodiment, the pharmaceutically acceptable excipients of the second composition comprises: i. Polyquaternium 10 in an amount of 1.0% w / w; ii. Glycerin in an amount of 5.0% w / w; 15 iii. Sodium lauryl ether sulphate in an amount of 45.0% w / w; iv. Sodium lauroyl sarcosinate in an amount of 2.0% w / w; v. Propylene glycol in an amount of 1.0% w / w; vi. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.1% w / w; vii. Biosaccharide Gum-1 in an amount of 0.5% w / w; 20 viii. Sodium chloride in an amount of 0.5% w / w; ix. Cyclopentasiloxane and Dimethiconol in an amount of 0.5% w / w; x. Perfume in an amount of 1.0% w / w; xi. Pearl powder in an amount of 0.1% w / w; xii. White powder in an amount of 0.1% w / w; 25 xiii. Disodium EDTA in an amount of 0.1% w / w; and xiv. Aqua In another embodiment, the active ingredient Anagain comprises of Pisum sativam sprout extract and Phenoxyethanol and Aqua. 30 In another embodiment, the additives of the second composition comprises: i. Kappaphycus alvarezii Extract in an amount of 0.5% w / w; ii. Vaccinium macrocarpon Fruit Extract (Cranberry extract) in an amount of 0.5% w / w; iii. Dextran Hydroxypropyltrimonium Chloride in an amount of 5.0% w / w; 35 and 5 iv. Saw palmetto extract in an amount of 1.0% w / w In an embodiment, the third composition comprises: i. Niacinamide in an amount of 0.5% w / w; ii. Onion Oil in an amount of 0.5% w / w; 10 iii. Procapil in an amount of 1.0% w / w; iv. Anagain in an amount of 0.5% w / w; v. Redensyl in an amount of 0.1% w / w; vi. Saw Palmetto Oil in an amount of 0.5% w / w; vii. Biotin in an amount of 0.1% w / w; and 15 viii. Melatonin in an amount of 0.01% w / w together with pharmaceutically acceptable excipients and additives in a suitable vehicle. In another embodiment, the pharmaceutically acceptable excipients of the third 20 composition comprises: i. Glycerin in an amount of 5.0 % w / w; ii. Phenoxyethanol and Ethylhexylglycerin in an amount of 1.0% w / w; iii. Biosaccharide Gum-1 in an amount of 1.0% w / w; iv. Sodium Metabisulfite in an amount of 0.5% w / w; 25 v. Hydroxyethyl Cellulose in an amount of 0.5% w / w; vi. Xanthan Gum in an amount of 0.5% w / w; vii. Perfume in an amount of 0.5% w / w; viii. Benzyl Alcohol in an amount of 0.5% w / w; ix. Triethanolamine in an amount of 0.2% w / w; 30 x. Disodium EDTA in an amount of 0.1% w / w; and xi. Aqua– Q.S The active ingredient Redensyl helps in hair stem cell multiplication, boosts hair growth and aids in the formation of strong hair fibres. Procapil strengthens the 35 hair by increasing the nutrition supply to the hair roots. Anagain prolongs the 5 anagen phase of hair cycle, therefore preventing premature shedding of hair. Niacinamide promotes circulation and improves the look and feel of hair by smoothness and shine. It also improves the texture of physically / chemically damaged hair by helping build keratin. Onion Oil which is rich in sulfur prevents breakage, split ends and thinning of hair. 10 In another embodiment, the active ingredient Anagain comprises of Pisum sativum (Pea) Sprout Extract, Alcohol and Aqua / Water. In yet another embodiment, the active ingredient Redensyl comprises of Glycerin, 15 Sodium Metabisulfite, Larix Europaea Wood Extract, Glycine, Zinc Chloride, Camellia Sinensis Leaf Extract and Aqua. In another embodiment, the active ingredient Procapil comprises of Butylene Glycol, PPG-26-Buteth-26, PEG-40 Hydrogenated Castor Oil, Apigenin, 20 Oleanolic Acid, Biotinoyl Tripeptide-1 and Aqua. In another embodiment, the additives of the third composition comprises: i. Capixyl in an amount of 5.0% w / w; ii. Aminexil in an amount of 1.5% w / w; 25 iii. Caffeine in an amount of 0.005% w / w; iv. Caprylic / capric triglyceride in an amount of 2.0% w / w; v. Ginseng extract in an amount of 5.0% w / w; and vi. Ginkgo biloba extractin an amount of 5.0% w / w 30 In another embodiment, the fourth composition comprises: i. Vitamins in an amount of 1.5 % w / w; ii. Metal salts in an amount of 0.3 % w / w; iii. Plant extracts in an amount of 6 % w / w; iv. Amino acids in an amount of 1.5 % w / w; 35 v. Pigments in an amount of 0.3 % w / w; 5 vi. Probiotics in an amount of 0.1 % w / w; and vii. Omega fatty acids in an amount of 0.5 % w / w; In an embodiment, the Vitamins of the fourth composition are selected from the group consisting of Vitamin B1, Vitamin B2, Vitamin B3, Vitamin B5, Vitamin 10 B6, Vitamin B7, Vitamin B9, Vitamin B12, Vitamin C, Vitamin D2, Vitamin E and the like. In another embodiment, the Metal salts of the fourth composition are selected from the group consisting of Magnesium oxide, dicalcium phosphate, calcium, 15 phosphorus, ferrous bisglycinate, zinc sulphate and the like. In yet another embodiment, the plant extracts of the fourth composition are selected from the group consisting of Centella Asiatica leaf extract, Cranberry fruit dried extract, Holy basil, Fenugreek seed extract, Ginkgo biloba, grape seed 20 extract, ashwagandha root extract, pumpkin seed extract, Brahmi leaf extract, Cinnamon bark extract, coconut powder, ginger rhizome extract, glycyrrhiza glabra extract, rose mary leaves extract, Beta sitosterol and the like. In another embodiment, the amino acids of the fourth composition are selected25 from the group consisting of N-Acetyl-Cysteine, L-Ornithine, L-lysine HCl, L- Histidine HCl and the like. In yet another embodiment, the pigments of the fourth composition are selected from the group consisting of Lutein, Chromium picolinate, lycopene, Citrus 30 bioflavonoids, and the like. In a further embodiment, the omega fatty acids of the fourth composition are selected from Omega 3 fatty acid, gamma-linoleic acid and the like. 5 In a further embodiment, the probiotics of the fourth composition are selected from Lactobacillus acidophilus and the like. In another embodiment, the first composition may be formulated in the form of a solution in a vial, or more preferably may be formulated as prefilled syringes for 10 better penetration into the scalp. In another embodiment, the second composition may be formulated in the form of serum, shampoo, hair oil, lotion, gels, spray, cream, tablets, capsules and the like; more preferably as shampoo. 15 In another embodiment, the third composition may be formulated in the form of serum, shampoo, hair oil, lotion, gels, spray, cream, tablets, capsules and the like; more preferably as serum. 20 In another embodiment, the fourth composition may be formulated in the form of serum, shampoo, hair oil, lotion, gels, spray, cream, tablets, capsules, syrup and the like; more preferably as tablets or capsules. In another embodiment, the hair growth promoting kit minimizes hair breakage 25 and split ends and nourishes the hair roots thereby prevents hair loss and improves hair growth. In an embodiment, the method of promoting hair growth and preventing early hair loss and for strengthening, nourishing, rejuvenating hair to improve the bodily 30 appearance of a person comprises applying the compositions provided in the kit as directed in the instruction manual. Accordingly, the method comprises the following steps: i. Administering the first composition through standard microneedling techniques including but not limited to dermaroller, dermapen, dermatok, 35 MNRF; 5 ii. Applying the second composition topically, in a suggested volume of 2- 3mL, twice daily; iii. Applying the third composition topically before rinsing hair with water, by gently massaging onto the scalp followed by thoroughly washing off the scalp; and 10 iv. orally administering the fourth composition , 2 tablets taken once daily, preferably in the night before sleeping. In another embodiment, the hair growth promoting kit is useful in the prevention of early hair loss by stimulating hair follicles, strengthening, nourishing, 15 rejuvenating and retention of hair to improve the bodily appearance of a person. In another embodiment, the compositions of the hair growth promoting kit of the present invention are stable and do not produce any harmful / side effects on application to the scalp. 20 The present kit for promoting hair growth has the following advantages: ^ Nourishes the hair roots and strengthens the hair follicles. ^ Prolongs the hair growth phase and promotes hair growth. ^ Improves hair shaft thickness to give more voluminous hair. 25 ^ Minimizes hair breakage and split ends and nourishes the hair. ^ Restores shiny and bouncy hair. ^ Hydrates the hair from the scalp to the tips. The invention can be better understood by the following non-limiting examples. 30 The examples given are mere an illustration of the instant invention and should not be construed as limiting the scope of the present invention in any manner. Examples: Example 1a: Preparation of the first composition: 5 The process for the preparation of the first composition which includes the following steps: 1) The solvent i.e. water at a temperature of 25°C is added with the conditioning ingredients of the composition to the disperser; 2) The disperser is set at 800 rpm for 30 minutes. 10 3) pH is adjusted, and a 500 MESH micron filter system is used to filter the final product. This is followed by filling, closing, and labeling of the vials. Example 1b: 15 Table 1: First composition Sr. Name of Ingredient Concentration No. 1 Vascular Endothelial Growth Factor (Human 5 mg / L oligopeptide-11) including its biomimetic analogue Sh-Polypeptide-9 2 Basic Fibroblast Growth Factor (Human 2 mg / L oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1 3 Insulin-like Growth Factor 1 (Human 2 mg / L oligopeptide-2) including its biomimetic analogue Sh-Oligopeptide-2 4 Copper Tripeptide 1 10 mg / L 5 Keratinocyte Growth Factor including its 1 mg / L biomimetic analogue Sh-Polypeptide-3 6 Thymosin-β4 including its biomimetic analogue 0.01 mg / L Sh-Oligopeptide-4 Example 2a: Preparation of the second composition: 5 The active ingredients Anagain, Caffeine, Vitamin B5, Vitamin E and Citric acid were added in the desired proportions in combination with pharmaceutically / cosmetically acceptable and appropriate dose of Polyquaternium 10, Glycerin, Sodium lauryl ether sulphate, Sodium lauroyl sarcosinate, Propylene glycol, Phenoxyethanol and Ethylhexylglycerin, Biosaccharide Gum-1, Sodium chloride, 10 Cyclopentasiloxane and Dimethiconol, Perfume, Pearl powder, White powder, Disodium EDTA, and water. The composition was then biologically sterilized and bottled. Example 2b: 15 1.0% w / w of Anagain, 1.0% w / w of Caffeine, 0.5% w / w of Vitamin B5, 0.1% w / w of Vitamin E, 0.01% w / w of Citric acid, 1.0% w / w of Polyquaternium 10, 5.0% w / w of Glycerin, 45.0% w / w of Sodium lauryl ether sulphate, 2.0% w / w of Sodium lauroyl sarcosinate, 1.0% w / w of Propylene glycol, 0.1% w / w of Phenoxyethanol and Ethylhexylglycerin, 0.5% w / w of Biosaccharide Gum-1, 20 0.5% w / w of Sodium chloride, 0.5% w / w of Cyclopentasiloxane and Dimethiconol, 1.0% w / w of Perfume, 0.1% w / w of Pearl powder, 0.1% w / w of White powder and 0.1% w / w of Disodium EDTA were added to 1 litre of distilled water, further disclosed in Table 2. The composition was then biologically sterilized and bottled. 25 Table 2: Second composition Sr. No. Name of Ingredient Concentration 1 Anagain 1.0% w / w 2 Caffeine 1.0% w / w 3 Polyquaternium 10 1.0% w / w 4 Glycerin 5.0% w / w 5 Sodium lauryl ether sulphate 45.0% w / w 6 Sodium lauroyl sarcosinate 2.0% w / w 7 Propylene glycol 1.0% w / w 8 Phenoxyethanol and Ethylhexylglycerin 0.1% w / w 9 Biosaccharide Gum-1 0.5% w / w 10 Sodium chloride 0.5% w / w 11 Cyclopentasiloxane and Dimethiconol 0.5% w / w 12 Perfume 1.0% w / w 13 Vitamin B5 0.5% w / w 14 Pearl powder 0.1% w / w 15 White powder 0.1% w / w 16 Vitamin E 0.1% w / w 17 Disodium EDTA 0.1% w / w 18 Citric acid 0.01% w / w 19 Aqua Q.S 5 The second composition may further comprise the following additives as listed in Table 3 given below. Table 3 Sr. No. Name of Ingredient Concentration 10 1 Kappaphycus alvarezii Extract 0.5% (5mg / mL) 2 Vaccinium macrocarpon Fruit 0.5% (5mg / mL) Extract (Cranberry extract) 3 Dextran Hydroxypropyltrimonium 5% (50mg / mL) 15 Chloride 4 Saw palmetto extract 1% (1mg / mL) 20 Example 3a: Preparation of the third composition: The active ingredients Niacinamide, Onion Oil, Procapil, Anagain, Redensyl were added in the desired proportions in combination with pharmaceutically / 5 cosmetically acceptable and appropriate dose of Saw Palmetto Oil, Biotin, Melatonin, Glycerin, Phenoxyethanol and Ethylhexylglycerin, Biosaccharide Gum-1, Sodium Metabisulfite, Hydroxyethyl Cellulose, Xanthan Gum, Perfume, Benzyl Alcohol, Triethanolamine, Disodium EDTA and water. The composition was then biologically sterilized and bottled. 10 Example 3b: Preparation of the third composition: 0.5% w / w of Niacinamide, 0.5% w / w of Onion Oil, 1.0% w / w of Procapil, 0.5% w / w of Anagain, 0.1% w / w of Redensyl, 0.5% w / w of Saw Palmetto Oil, 0.1% w / w of Biotin, 0.01% w / w of Melatonin, 5.0 % w / w of Glycerin, 1.0% w / w of 15 Phenoxyethanol and Ethylhexylglycerin, 1.0% w / w of Biosaccharide Gum-1, 0.5% w / w of Sodium Metabisulfite, 0.5% w / w of Hydroxyethyl Cellulose, 0.5% w / w of Xanthan Gum, 0.5% w / w of Perfume, 0.5% w / w of Benzyl Alcohol, 0.2% w / w of Triethanolamine, 0.1% w / w of Disodium EDTA and water added to 1 litre of distilled water, further disclosed in Table 4. The composition was then 20 biologically sterilized and bottled. Table 4: Third composition Sr. No. Name of Ingredient Concentration 1 Procapil 1.0% w / w 2 Anagain 0.5% w / w 3 Redensyl 0.1% w / w 4 Niacinamide 0.5% w / w 5 Onion Oil 0.5% w / w 6 Saw Palmetto Oil 0.5% w / w 7 Biotin 0.1% w / w 8 Melatonin 0.01% w / w 9 Glycerin 5.0 % w / w 10 Phenoxyethanol and Ethylhexylglycerin 1.0% w / w 11 Biosaccharide Gum-1 1.0% w / w 12 Sodium Metabisulfite 0.5% w / w 13 Hydroxyethyl Cellulose 0.5% w / w 14 Xanthan Gum 0.5% w / w 15 Perfume 0.5% w / w 16 Benzyl Alcohol 0.5% w / w 17 Triethanolamine 0.2% w / w 18 Disodium EDTA 0.1% w / w 19 Aqua Q.S 5 The third composition may further comprise the following additives as listed in Table 5 given below. Table 5 Sr. No Name of Ingredient Concentration 1 Capixyl 5% (50mg / mL) 2 Aminexil 1.5% (15mg / mL) 3 Caffeine 0.005% (0.05mg / mL) 4 Caprylic / capric triglyceride 2% (20mg / mL) 5 Ginseng extract 5% (50mg / mL) 6 Ginkgo biloba extract 5% (50mg / mL) 10 Example 4a: Preparation of fourth composition: The active ingredients Vitamins, metal salts, plant extracts, amino acids, pigments, probiotics and Omega fatty acid were added in the desired proportions. The composition was then biologically sterilized and bottled. 15 Example 4b: 5 Vitamins, metal salts, plant extracts, amino acids, pigments, probiotics and Omega fatty acid added to 1 litre of distilled water, further disclosed in Table 6. The composition was then biologically sterilized and bottled. Table 6: Fourth composition Sr. No Name of Ingredient Concentration 1 Vitamins 1.5 % w / w 2 Metal salts 0.3 % w / w 3 Plant extracts 6 % w / w 4 Amino acids 1.5 % w / w 5 Pigments 0.3 % w / w 6 Probiotics 0.1 % w / w 7 Omega fatty acid 0.5 % w / w 10 Table 7: Ingredients Information (Approximate Values) PROTECT 15 Nutritional Information Per Serving (Approximate Values) Nutrients Per % RDA Serving Per Serving 1 Tablet Energy 0.44 Kcal 0.01% Fats & its derivatives 0 ** Proteins 0 ** Carbohydrates 0.11 g 0.08% Ingredients Information (Approximate Values) Dicalcium Phosphate 500 mg Calcium 115 mg 11.5% Phosphorous 95 mg 15.83% Ashwagandha Root 150 mg ** Ext., Magnesium Oxide 50 mg 6.85% Grape Seed Ext. 50 mg ** Beta Sitosterol 50 mg ** Ginseng Root Ext. 42.5 mg ** Vitamin C (L- 40 mg 50% AscorbicAcid) Zinc Sulphate 20 mg 47.6% Vitamin B3 18 mg 100% (Nicotinamide) Vitamin E (D alpha 10 mg 100% tocopheryl acetate) Ginkgo Biloba Dried 5 mg ** Leaves Extract Vitamin B5 (Calcium 4 mg 80% Pantothenate) Vitamin B6 (Pyridoxine 2 mg 83.3% HCl) Cupric Sulphate 2 mg 46.83% Vitamin B2 1.6 mg 64% (Riboflavin) Vitamin B1 (Thiamin 1.4 mg 77.7% Mononitrate) Manganese Sulphate 0.5 mg 4.54% Lactic Acid Bacillus 0.5 mg ** Vitamin A (Retinyl 0.3 mg 30% Palmitate) Vitamin B9 (Folic Acid) 176.5 mcg 100% Vitamin K1 30 mcg 54.5% (Phylloquinone) Chromium Picolinate 25 mcg 6.21% Vitamin D2 200 I.U. 66.6% (Ergocalcifero) Vitamin B12 1 mcg 45.45% (Cyanocobalamin) Lutein 6% 0.1 mcg ** Excipients q.s. 5 ** Recommended Daily Allowance not met ENHANCE Nutrients Per %RDA Serving Per serving 1 Tablet Energy 0.73 Kcal 0.036% Fats & its derivatives 0.01g 0.01% Proteins 0.1g 0.18% Carbohydrates 0.06g ** Ingredients Information (Approximate Values) Pumpkin Seed Extract 300 mg ** Omega 3 Fatty Acid 300 mg ** (Flax Seed Powder) Ashwagandha Root 100 mg ** Extract Brahmi Leaf Extract 100 mg ** Ferrous Bisglycinate 60 mg 63.15% Beta Sitosterol 10% 50 mg ** Cinnamon Extract 30 mg ** Coconut Powder 30 mg ** Zinc Sulphate 30 mg 71.46% Ginger extracts 30 mg ** Glycyrrhiza Glabra 30 mg ** Rosemary Extract 30 mg ** L-Lysine 25 mg 1.38% Monohydrochloride Bioflavonoids 40% 15 mg ** Gamma Linolenic Acid 10 mg ** Grape Seed Extract 10 mg ** Vitamin B5 (Calcium D 5 mg 100% Pantothenate) L-Histidine Mono 4 mg 0.61% Hydrochloride Lycopene 6% 200 mcg ** Vitamin B9 (Folic 176.6 mg 100% Acid) Vitamin B12 1 mcg 45.45% (Cyanocobalamin) Excipients q.s. 5 ** Recommended Daily Allowance not met Maintain Nutrients Per %RDA Serving Per serving 1 Tablet Energy 1.4 Kcal 0.07 % Fats & its derivatives 0.002 g 0.002 % Proteins 0.3 g 0.55 % Carbohydrates 0.05 g ** Ingredients Information (Approximate Values) L-Taurine 200 mg ** Ashwagandha Root 125 mg ** Extract Green Tea Extract 75 mg ** Beta Sitosterol 10% 50 mg ** Inositol 50 mg ** Cinnamon Bark Powder 50 mg ** Amla Fruit extract 50 mg ** Aegle marmelose (bael) 30 mg ** Centella asiatica leaf 30 mg ** extract Cranberry fruit dried 30 mg ** extract Hoky basil 30 mg ** Fenugreek seed extract 25 mg ** N-Acetyl L-Cysteine 25 mg ** L-ornithine 25 mg ** D-Alpha Tocopherol 10 mg 100% Acetate Calcium Pantothenate 5 mg 100% Pyridoxine HCl 2 mg 83.33% Selenomethionine 100 mcg 100% Biotin (D-Biotin) 30 mcg 75% Excipients q.s. Amino Acid Profile per 330 mg of protein hydrolysate (approximate values) L-glutamic acid 55 ** DL-methionine 40 6.66% L-aspartic Acid 36.3 ** L-leucine 20.6 0.88% L-Arginine 20.3 ** L-Lysine 18.2 1.01% L-Proline 16 ** L-Serine 16 ** L-Phenylalanine 16 1.06% L-Isoleucine 14.6 1.21% L-Valine 14 0.89% L-Glycine 112.8 ** L-Alanine 11.9 ** L-Threonine 11.3 1.25% L-Histidine 7.7 1.25% L-Cystine 3.9 1.62% L-Tryptophan 3.6 1.5% 5 ** Recommended Daily Allowance not met 5 Example 5: The following tests were conducted with the present kit components mentioned in examples 1 to 4b to check the improvement in 1) Strengthening of hair follicles: A hair pull test was conducted at 10 baseline, 3 months and 6 months from the start of therapy. The average number of hair pulled at baseline was 10.5, which reduced to 4 at 3 months and 2.35 at 6 months. Indicating a negative hair-pull test post-therapy. 2) Increase in hair growth: Global photographic assessment of subjects 15 undergoing therapy was conducted at baseline, 3 months and 6 months. The global photography assessment score was 4.5 at baseline, which gradually increased to 6 at 3 months and 8 at 6 months. 3) Increase in terminal hair count: Videomicroscopic assessment was 20 conducted. There was significant increase in terminal hair count and decrease in the vellus hair count in 82% of the subjects. 4) Hair Shaft Diameter: The average hair shaft diameter for each patient, taken at 15 cm from the glabella, 57% patients had an increase in the width 25 of their hairs. Paired t-testing indicated that after 3 months, patients had an average hair shaft diameter that was 20 um wider and 50 um wider after 6 months than at baseline. This was statistically significant. 5) Restoration of shiny hair and hydrated hair: On self-evaluation, 84% of 30 the patients reported restoration of shiny and bouncy hair and 76% of the patients reported decrease in dryness of hair. Table 8 Variables Outcome QR678 Control T Outcome Mean +SD Level of Mean Level of value significance +SD significance Terminal hair Baseline 65.42 ± 0.0001 66.23 ± 0.0001 25.40 count (cm2) 1.7 1.9 Final 82.50 ± 64.44± 11.19 2.9 2.6 Vellus hair Baseline 38.54 ± 0.0001 37.43 ± 0.0001 22.73 count (cm2) 3.1 2.8 Final 20.43 ± 38.19 ± 10.86 2.5 1.2 Hair density Baseline 106.5 ± 0.0001 105.2 ± 0.0001 38.05 (cm2) 2.3 1.6 Final 179.7 ± 102.5 ± 13.41 2.0 2.2 Shaft Baseline 30.21±2.0 0.0001 29.70 ± 0.0001 21.49 diameter (μm) 3.1 Final 44.31 ± 24.0 ± 8.2 2.6 1.5 5 Example 6: Global Hair Growth and Global Hair Quality Improvement: Table 9 depicts the change in Global Hair Growth and Global Hair Quality Improvement score post-therapy as compared to baseline. 10 Table 9: Global Hair Growth and Global Hair Quality Improvement BASELINE POST-THERAPY FEMALES MALES FEMALES MALES n= 200 n= 200 n= 200 n= 200 (%) (%) (%) (%) Overall 1 166 158 7 6 appearance (worsened) (83.0%) (79.0%) (3.5%) (3.0%) 2 32 40 25 18 (neutral) (16%) (20.0%) (12.5%) (9.0%) 3 2 2 168 176 (improved) (1.0%) (1.0%) (84.0%) (88.0%) Scalp 1 166 164 7 7 coverage (worsened) (83.0%) (82.0%) (3.5%) (3.5%) 2 32 31 25 19 (neutral) (16.0%) (15.5%) (12.5%) (9.5%) 3 2 5 168 174 (improved) (1.0%) (2.5%) (84.0%) (87.0%) Texture of 1 165 159 7 6 hair (worsened) (82.5%) (79.5%) (3.5%) (3.0%) 2 33 38 25 19 (neutral) (16.5%) (19.0%) (12.5%) (9.5%) 3 2 3 168 175 (improved) (1.0%) (1.5%) (84.0%) (87.5%) Hair 1 166 163 7 6 dryness (worsened) (83.0%) (81.5%) (3.5%) (3.0%) 2 32 33 21 20 (neutral) (16.0%) (16.5%) (10.5%) (10.0%) 3 2 4 172 174 (improved) (1.0%) (2.0%) (86.0%) (87.0%) Hair shine1165 159 7 5 (worsened) (82.5%) (79.5%) (3.5%) (2.5%) 2 32 36 22 21 (neutral) (16.0%) (18.0%) (11.0%) (10.5%) 3 3 5 171 174 (improved) (1.5%) (2.5%) (85.5%) (87.0%) Brittleness1165 167 7 6 (worsened) (82.5%) (83.5%) (3.5%) (3.0%) 2 33 32 27 19 (neutral) (16.5%) (16.0%) (13.5%) (9.5%) 3 2 1 166 175 (improved) (1.0%) (0.5%) (83.0%) (87.5%) 5 Results: The mean Global Hair Growth and Global Hair Quality Improvement scores for the males pre-intervention were 7.25 ^ 1.08 and for females were 7.10 ^ 1.19; whereas post-therapy the mean values were found to be 17.06 ^ 1.19 in males and 16.82 ^ 1.04 in females. 10 Although the invention has been described in detail in the foregoing for the purpose of illustration, it is to be understood that such detail is solely for that purpose and that variations can be made therein by those skilled in the art without departing from the spirit and scope of the invention except as it may be limited by the claims. 15 20 25 30

Claims

5 We Claim, 1. A kit for promoting hair growth and preventing hair loss, comprising: i. a first composition consisting of Vascular Endothelial Growth Factor (Human oligopeptide-11) including its biomimetic analogue Sh- 10 Polypeptide-9, Basic Fibroblast Growth Factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1, Insulin-like Growth Factor 1 (Human oligopeptide-2) including its biomimetic analogue Sh-Oligopeptide-2, Copper Tripeptide 1, Keratinocyte Growth Factor including its biomimetic analogue Sh-15 Polypeptide-3, Thymosin-β4 including its biomimetic analogue Sh- Oligopeptide-4; ii. a second composition consisting of Anagain, Caffeine, Vitamin B5, Vitamin E and Citric acid; together with pharmaceutically acceptable excipients and additives; 20 iii. a third composition consisting of Niacinamide, Onion Oil, Procapil, Anagain, Redensyl, Saw Palmetto Oil, Biotin and Melatonin; together with pharmaceutically acceptable excipients and additives; iv. a fourth composition consisting of nutraceuticals together with pharmaceutically acceptable excipients; and 25 v. an instruction manual 2. The kit as claimed in Claim 1, wherein said first composition comprises: i. Vascular endothelial growth factor (Human oligopeptide-11) including its biomimetic analogue Sh-Polypeptide-9 in an amount of 0.01mg / L – 100mg / L; 30 ii. Basic fibroblast growth factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1 in an amount of 0.01mg / L – 100mg / L; iii. Insulin like growth factor (Human oligopeptide-2) including its biomimetic analogue Sh-Oligopeptide-2 in an amount of 0.01mg / L – 35 100mg / L;5 iv. Copper tripeptide 1 in an amount of 0.1mg / L – 500 mg / L; v. Keratinocyte growth factor including its biomimetic analogue Sh- Polypeptide-3 in an amount of 0.01mg / L – 100 mg / L; and vi. Thymosin β4 including its biomimetic analogue Sh-Oligopeptide-4 in an amount of 0.005mg / L – 100 mg / L. 10 3. The kit as claimed in Claim 1, wherein said second composition comprises: i. Anagain in an amount of 0.5 - 3.0% w / w; ii. Caffeine in an amount of 0.5 - 3.0% w / w; iii. Vitamin B5 in an amount of 0.2 – 1.0 % w / w; 15 iv. Vitamin E in an amount of 0.01 – 0.5 % w / w; and v. Citric acid in an amount of 0.005 – 0.05 % w / w; together with pharmaceutically acceptable excipients and additives in a suitable vehicle.

4. The kit as claimed in Claim 1and 3, wherein said pharmaceutically 20 acceptable excipients in the second composition comprises: i. Polyquaternium 10 in an amount of 0.5 - 3.0% w / w; ii. Glycerin in an amount of 2.0 – 7.0 % w / w; iii. Sodium lauryl ether sulphate in an amount of 30 – 60% w / w; iv. Sodium lauroyl sarcosinate in an amount of 1.0 – 3.0% w / w; 25 v. Propylene glycol in an amount of 0.5 - 3.0% w / w; vi. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.01 – 0.5 % w / w; vii. Biosaccharide Gum-1 in an amount of 0.2 – 1.0 % w / w; viii. Sodium chloride in an amount of 0.2 – 1.0 % w / w; 30 ix. Cyclopentasiloxane and Dimethiconol in an amount of 0.2 – 1.0 % w / w; x. Perfume in an amount of 0.5 - 3.0% w / w; xi. Pearl powder in an amount of 0.01 – 0.5 % w / w; xii. White powder in an amount of 0.01 – 0.5 % w / w; 35 xiii. Disodium EDTA in an amount of 0.01 – 0.5 % w / w; and5 xiv. Aqua- Q.S 5. The kit as claimed in Claim 3, wherein said active ingredient Anagain comprises of Pisum sativam sprout extract and Phenoxyethanol and Aqua.

6. The kit as claimed in Claim 1 and 3, wherein said additives in the second composition comprises: 10 i. Kappaphycus Alvarezii Extract in an amount of 0.1 – 1.0 % w / w; ii. Vaccinium Macrocarpon Fruit Extract (Cranberry extract) in an amount of 0.1 – 1.0 % w / w; iii. Dextran Hydroxypropyltrimonium Chloride in an amount of 1.0 – 10 % w / w; and 15 iv. Saw palmetto extract in an amount of 0.005 – 5 % w / w.

7. The kit as claimed in Claim 1, wherein said third composition comprises: i. Niacinamide in an amount of 0.2 – 1.0 % w / w; ii. Onion Oil in an amount of 0.2 – 1.0 % w / w; iii. Procapil in an amount of 0.5 - 3.0% w / w; 20 iv. Anagain in an amount of 0.2 – 1.0 % w / w; v. Redensyl in an amount of 0.05 – 0.5% w / w; vi. Saw Palmetto Oil in an amount of 0.2 – 1.0 % w / w; vii. Biotin in an amount of 0.01 – 0.5 % w / w; and viii. Melatonin in an amount of 0.005 – 0.05 % w / w; 25 together with pharmaceutically acceptable excipients and additives in a suitable vehicle.

8. The kit as claimed in Claim 7, wherein said active ingredient Anagain comprises of Pisum sativum (Pea) Sprout Extract, Alcohol and Aqua / Water. 30 9. The kit as claimed in Claim 7, wherein said active ingredient Redensyl comprises of Glycerin, Sodium Metabisulfite, Larix Europaea Wood Extract, Glycine, Zinc Chloride, Camellia Sinensis Leaf Extract and Aqua.

10. The kit as claimed in Claim 7, wherein said active ingredient Procapil comprises of Butylene Glycol, PPG-26-Buteth-26, PEG-40 Hydrogenated 35 Castor Oil, Apigenin, Oleanolic Acid, Biotinoyl Tripeptide-1 and Aqua.5 11. The kit as claimed in Claim 1 and 7, wherein said pharmaceutically acceptable excipients in the third composition comprises: i. Glycerin in an amount of 2.0 – 7.0 % w / w; ii. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.5 – 3.0 % w / w; 10 iii. Biosaccharide Gum-1 in an amount of 0.5 – 3.0 % w / w; iv. Sodium Metabisulfite in an amount of 0.2 – 1.0 % w / w; v. Hydroxyethyl Cellulose in an amount of 0.2 – 1.0 % w / w; vi. Xanthan Gum in an amount of 0.2 – 1.0 % w / w; vii. Perfume in an amount of 0.2 – 1.0 % w / w; 15 viii. Benzyl Alcohol in an amount of 0.2 – 1.0 % w / w; ix. Triethanolamine in an amount of 0.1 – 0.5% w / w; x. Disodium EDTA in an amount of 0.01 – 0.5 % w / w; and xi. Aqua– Q.S 12. The kit as claimed in Claim 1 and 7, wherein said additives in the third 20 composition comprises: i. Capixyl in an amount of 1.0 – 10 % w / w; ii. Aminexil in an amount of 0.5 – 5.0 % w / w; iii. Caffeine in an amount of 0.001 – 0.01 % w / w; iv. Caprylic / capric triglyceride in an amount of 0.5 – 5.0 % w / w; 25 v. Ginseng extract in an amount of 1.0 – 10 % w / w; and vi. Ginkgo biloba extract in an amount of 1.0 – 10 % w / w.

13. The kit as claimed in Claim 1, wherein said fourth composition comprises nutraceuticals including but is not limited to vitamins, metal salts, plant extracts, amino acids, pigments, probiotics, Omega fatty acids and the like. 30 14. The kit as claimed in Claim 1, wherein said fourth composition comprises: i. Vitamins in an amount of 1-2 % w / w; ii. Metal salts in an amount of 0.2-0.5 % w / w; iii. Plant extracts in an amount of 5-8 % w / w; iv. Amino acids in an amount of 1-2 % w / w; 35 v. Pigments in an amount of 0.2-0.5 % w / w;5 vi. Probiotics in an amount of 0.1-0.2 % w / w; and vii. Omega fatty acids in an amount of 0.4-0.6 % w / w.

15. The kit as claimed in Claim 14, wherein said Vitamins are selected from the group consisting of Vitamin B1, Vitamin B2, Vitamin B3, Vitamin B5, Vitamin B6, Vitamin B7, Vitamin B9, Vitamin B12, Vitamin C, Vitamin 10 D2, Vitamin E and the like.

16. The kit as claimed in Claim 14, wherein said Metal salts are selected from the group consisting of Magnesium oxide, dicalcium phosphate, calcium, phosphorus, ferrous bisglycinate, zinc sulphate and the like.

17. The kit as claimed in Claim 14, wherein said plant extracts are selected 15 from the group consisting of Centella asiatica leaf extract, Cranberry fruit dried extract, Holy basil, Fenugreek seed extract, Ginkgo biloba, grape seed extract, ashwagandha root extract, pumpkin seed extract, Brahmi leaf extract, Cinnamon bark extract, coconut powder, ginger rhizome extract, glycyrrhiza glabra extract, rose mary leaves extract, Beta sitosterol and the 20 like.

18. The kit as claimed in Claim 14, wherein said amino acids are selected from the group consisting of N-Acetyl-Cysteine, L-Ornithine, L-lysine HCl, L-Histidine HCl and the like.

19. The kit as claimed in Claim 14, wherein said pigments are selected from 25 the group consisting of Lutein, Chromium picolinate, lycopene, Citrus bioflavonoids and the like.

20. The kit as claimed in Claim 14, wherein said omega fatty acids are selected from Omega 3 fatty acid, gamma-linoleic acid and the like.

21. The kit as claimed in Claim 14, wherein said probiotics are selected from 30 Lactobacillus acidophilus and the like.

22. The kit as claimed in Claim 1, wherein said first composition comprises: i. Vascular endothelial growth factor (Human oligopeptide-11) including its biomimetic analogue Sh-Polypeptide-9 in an amount of5 ii. Basic fibroblast growth factor (Human oligopeptide-3) including its biomimetic analogue Sh-Polypeptide-1 in an amount of 2 mg / L; iii. Insulin like growth factor (Human oligopeptide-2) including its biomimetic analogue Sh-Oligopeptide-2 in an amount of 2 mg / L; iv. Copper tripeptide 1 in an amount of 10 mg / L; 10 v. Keratinocyte growth factor including its biomimetic analogue Sh- Polypeptide-3 in an amount of 1 mg / L; and vi. Thymosin β4 including its biomimetic analogue Sh-Oligopeptide-4 in an amount of 0.01 mg / L.

23. The kit as claimed in Claim 1, wherein said second composition 15 comprises: i. Anagain in an amount of 1.0% w / w; ii. Caffeine in an amount of 1.0% w / w; iii. Vitamin B5 in an amount of 0.5% w / w; iv. Vitamin E in an amount of 0.1% w / w; and 20 v. Citric acid in an amount of 0.01% w / w; together with pharmaceutically acceptable excipients and additives in a suitable vehicle.

24. The kit as claimed in Claim 1 and 23, wherein said pharmaceutically acceptable excipients in the second composition comprises: 25 i. Polyquaternium 10 in an amount of 1.0% w / w; ii. Glycerin in an amount of 5.0% w / w; iii. Sodium lauryl ether sulphate in an amount of 45.0% w / w; iv. Sodium lauroyl sarcosinate in an amount of 2.0% w / w; v. Propylene glycol in an amount of 1.0% w / w; 30 vi. Phenoxyethanol and Ethylhexylglycerin in an amount of 0.1% w / w; vii. Biosaccharide Gum-1 in an amount of 0.5% w / w; viii. Sodium chloride in an amount of 0.5% w / w; ix. Cyclopentasiloxane and Dimethiconol in an amount of 0.5% w / w; x. Perfume in an amount of 1.0% w / w; 35 xi. Pearl powder in an amount of 0.1% w / w;5 xii. White powder in an amount of 0.1% w / w; xiii. Disodium EDTA in an amount of 0.1% w / w; and xiv. Aqua.

25. The kit as claimed in Claim 1 and 23, wherein said additives in the second composition comprises: 10 i. Kappaphycus alvarezii Extract in an amount of 0.5% w / w; ii. Vaccinium macrocarpon Fruit Extract (Cranberry extract) in an amount of 0.5% w / w; iii. Dextran Hydroxypropyltrimonium Chloride in an amount of 5.0% w / w; and 15 iv. Saw palmetto extract in an amount of 1.0% w / w.

26. The kit as claimed in Claim 23, wherein said active ingredient Anagain comprises of Pisum sativam sprout extract and Phenoxyethanol and Aqua.

27. The kit as claimed in Claim 1, wherein said third composition comprises: i. Niacinamide in an amount of 0.5% w / w; 20 ii. Onion Oil in an amount of 0.5% w / w; iii. Procapil in an amount of 1.0% w / w; iv. Anagain in an amount of 0.5% w / w; v. Redensyl in an amount of 0.1% w / w; vi. Saw Palmetto Oil in an amount of 0.5% w / w; 25 vii. Biotin in an amount of 0.1% w / w; and viii. Melatonin in an amount of 0.01% w / w together with pharmaceutically acceptable excipients and additives in a suitable vehicle.

28. The kit as claimed in Claim 1 and 27, wherein said pharmaceutically 30 acceptable excipients in the third composition comprises: i. Glycerin in an amount of 5.0 % w / w; ii. Phenoxyethanol and Ethylhexylglycerin in an amount of 1.0% w / w; iii. Biosaccharide Gum-1 in an amount of 1.0% w / w; iv. Sodium Metabisulfite in an amount of 0.5% w / w; 35 v. Hydroxyethyl Cellulose in an amount of 0.5% w / w;5 vi. Xanthan Gum in an amount of 0.5% w / w; vii. Perfume in an amount of 0.5% w / w; viii. Benzyl Alcohol in an amount of 0.5% w / w; ix. Triethanolamine in an amount of 0.2% w / w; x. Disodium EDTA in an amount of 0.1% w / w; and 10 xi. Aqua– Q.S 29. The kit as claimed in Claim 1 and 27, wherein said additives in the third composition comprises: i. Capixyl in an amount of 5.0% w / w; ii. Aminexil in an amount of 1.5% w / w; 15 iii. Caffeine in an amount of 0.005% w / w; iv. Caprylic / capric triglyceride in an amount of 2.0% w / w; v. Ginseng extract in an amount of 5.0% w / w; and vi. Ginkgo biloba extractin an amount of 5.0% w / w.

30. The kit as claimed in Claim 27, wherein said active ingredient Anagain 20 comprises of Pisum sativum (Pea) sprout extract, alcohol and aqua / water.

31. The kit as claimed in Claim 27, wherein said active ingredient Redensyl comprises of glycerin, sodium metabisulfite, larix europaea wood extract, glycine, zinc chloride, camellia sinensis leaf extract and aqua.

32. The kit as claimed in Claim 27, wherein said active ingredient Procapil 25 comprises of butylene glycol, PPG-26-Buteth-26, PEG-40 hydrogenated castor oil, apigenin, oleanolic acid, biotinoyl tripeptide-1 and aqua.

33. The kit as claimed in Claim 1, wherein said fourth composition comprises: i. Vitamins in an amount of 1.5 % w / w; ii. Metal salts in an amount of 0.3 % w / w; 30 iii. Plant extracts in an amount of 6 % w / w; iv. Amino acids in an amount of 1.5 % w / w; v. Pigments in an amount of 0.3 % w / w; vi. Probiotics in an amount of 0.1 % w / w; and vii. Omega fatty acids in an amount of 0.5 % w / w.5 34. The kit as claimed in Claim 33, wherein said Vitamins are selected from the group consisting of Vitamin B1, Vitamin B2, Vitamin B3, Vitamin B5, Vitamin B6, Vitamin B7, Vitamin B9, Vitamin B12, Vitamin C, Vitamin D2, Vitamin E and the like.

35. The kit as claimed in Claim 33, wherein said Metal salts are selected from 10 the group consisting of magnesium oxide, dicalcium phosphate, calcium, phosphorus, ferrous bisglycinate, zinc sulphate and the like.

36. The kit as claimed in Claim 33, wherein said plant extracts are selected from the group consisting of Centella asiatica leaf extract, Cranberry fruit dried extract, Holy basil, Fenugreek seed extract, Ginkgo biloba, grape 15 seed extract, ashwagandha root extract, pumpkin seed extract, Brahmi leaf extract, Cinnamon bark extract, coconut powder, ginger rhizome extract, Glycyrrhiza glabra extract, rose mary leaves extract, Beta sitosterol and the like.

37. The kit as claimed in Claim 33, wherein said amino acids are selected 20 from the group consisting of N-Acetyl-Cysteine, L-Ornithine, L-lysine HCl, L-Histidine HCl and the like.

38. The kit as claimed in Claim 33, wherein said pigments are selected from the group consisting of Lutein, Chromium picolinate, lycopene, Citrus bioflavonoids, and the like. 25 39. The kit as claimed in Claim 33, wherein said omega fatty acids are selected from Omega 3 fatty acid, gamma-linoleic acid and the like.

40. The kit as claimed in any one of the preceding claims, wherein said first composition may be formulated in the form of a solution in a vial, or more preferably may be formulated as prefilled syringes. 30 41. The kit as claimed in any one of the preceding claims, wherein said second composition may be formulated in the form of serum, shampoo, hair oil, lotion, gels, spray, cream, tablets, capsules and the like.

42. The kit as claimed in Claim 41, wherein said second composition may be formulated in the form of shampoo.5 43. The kit as claimed in any one of the preceding claims, wherein said third composition may be formulated in the form of serum, shampoo, hair oil, lotion, gels, spray, cream, tablets, capsules and the like.

44. The kit as claimed in Claim 43, wherein said third composition may be formulated in the form of serum. 10 45. The kit as claimed in any one of the preceding claims, wherein said fourth composition may be formulated in the form of serum, shampoo, hair oil, lotion, gels, spray, cream, tablets, capsules and the like.

46. The kit as claimed in Claim 45, wherein said fourth composition may be formulated in the form of tablets or capsules. 15 47. A method of promoting hair growth and preventing hair loss comprising applying the compositions provided in the kit as directed in the manual.

48. The kit as claimed in any of the preceding claims, wherein said kit is useful in the prevention of early hair loss by stimulating hair follicles, promoting hair growth, strengthening, nourishing, rejuvenating and 20 retention of hair to improve the bodily appearance of a person. 25 30

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