Liver perfusion system, sterility testing method, and testing apparatus

Abstract

The present application discloses a liver perfusion system, comprising: an organ basin; a first circulatory tubing, comprising a first outflow tube and a first perfusion tube, the first outflow tube being configured for allowing perfusate to flow into the organ basin, and the first perfusion tube being configured for allowing perfusate to flow out from the first outflow tube; a second circulatory tubing, comprising a second outflow tube and a second perfusion tube, the second outflow tube being configured for allowing perfusate to flow into the organ basin, and the second perfusion tube being configured for allowing perfusate to flow out from the second outflow tube; a first tube connector, comprising a first opening, a second opening, and a third opening, the first opening being in communication with the first outflow tube, the second opening being in communication with the first perfusion tube, and the third opening being configured for communicating with a testing apparatus; and a second tube connector, comprising a fourth opening, a fifth opening, and a sixth opening, the fourth opening being in communication with the second outflow tube, the fifth opening being in communication with the second perfusion tube, and the sixth opening being configured for communicating with the testing apparatus. The liver perfusion system of the present application allows workers to easily perform sterility testing.

Description

Liver perfusion system, sterility detection method and detection device TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a liver perfusion system, a sterility detection method and a detection device. BACKGROUND

[0002] In the related art, when a liver is transplanted, the liver needs to be placed in a liver perfusion system for preservation, and then the liver is transferred into the human body. The liver perfusion system can provide blood and nutrients to the liver when perfusing the liver, so as to keep the liver active.

[0003] Further, after the liver perfusion system is produced, it needs to be sterilized. After sterilization is completed, the staff will perform sterility detection on the liver perfusion system to determine whether the liver perfusion system is sterile. The existing liver perfusion system usually uses a membrane filtration method for sterility detection. However, when the membrane filtration method is used to detect the sterility of the liver perfusion system, the liver perfusion system is not convenient for the staff to operate, thereby resulting in low efficiency of sterility detection. SUMMARY

[0004] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides a liver perfusion system which can facilitate the staff to perform sterility detection.

[0005] The present application also provides a sterility detection method.

[0006] The present application also provides a detection device.

[0007] The liver perfusion system according to the first aspect of the present application comprises:

[0008] An organ basin having a storage cavity for accommodating an organ;

[0009] A first circulation pipeline comprising a first output pipe and a first perfusion pipe, one end of the first output pipe being in communication with the organ basin, the other end of the first output pipe being in communication with the first perfusion pipe, the first output pipe being located outside the organ basin, the first perfusion pipe being located in the organ basin, the first output pipe being used for the perfusion liquid to flow from the organ basin, and the first perfusion pipe being used for the perfusion liquid to flow from the first output pipe;

[0010] The second circulation pipeline comprises a second output pipe and a second perfusion pipe, one end of the second output pipe is communicated with the organ basin, the other end of the second output pipe is communicated with the second perfusion pipe, the second output pipe is located outside the organ basin, the second perfusion pipe is located in the organ basin, the second output pipe is used for flowing the perfusion liquid from the organ basin, and the second perfusion pipe is used for flowing the perfusion liquid from the second output pipe.

[0011] The first pipe joint comprises a first opening, a second opening and a third opening which are communicated with each other, the first opening is communicated with the first output pipe, the second opening is communicated with the first perfusion pipe, and the third opening is used for being communicated with the detection device.

[0012] The second pipe joint comprises a fourth opening, a fifth opening and a sixth opening which are communicated with each other, the fourth opening is communicated with the second output pipe, the fifth opening is communicated with the second perfusion pipe, and the sixth opening is used for being communicated with the detection device.

[0013] The liver perfusion system provided by the embodiment of the present application has at least the following beneficial effects: the detection device is communicated with the third opening and the sixth opening respectively, so that the buffer solution can be placed in the storage cavity of the organ basin, and then the buffer solution is circulated, at this time, the buffer solution can pass through the storage cavity, the first output pipe, the first perfusion pipe, the second output pipe and the second perfusion pipe, and after the buffer solution is circulated for multiple times, whether the buffer solution is sterile can be detected, if the detection result of the buffer solution is that the buffer solution is not sterile, it can be determined that the liver perfusion system is not sterile, and the product is unqualified, otherwise, if the detection result of the buffer solution is that the buffer solution is sterile, it can be determined that the liver perfusion system is sterile, and the product is qualified. Specifically, since the third opening and the sixth opening of the liver perfusion system in the present application can be communicated with the detection device, when the liver perfusion system is sterile, the third opening and the sixth opening can be communicated with the detection device to be detected, so that the liver perfusion system can facilitate the sterile detection of the staff.

[0014] The liver perfusion system provided by some embodiments of the present application further comprises a first oxygenator and a second oxygenator, the first oxygenator is communicated with the first circulation pipeline, and the second oxygenator is communicated with the second circulation pipeline.

[0015] The liver perfusion system provided by some embodiments of the present application further comprises a first filter and a second filter, the first filter is communicated with the first circulation pipeline, and the second filter is communicated with the second circulation pipeline.

[0016] According to some embodiments of the liver perfusion system, the liver perfusion system further comprises a first driving member and a second driving member, the first driving member being in communication with the first circulation pipeline, the second driving member being in communication with the second circulation pipeline, the first driving member being configured to drive the perfusion solution to flow in the first circulation pipeline, and the second driving member being configured to drive the perfusion solution to flow in the second circulation pipeline.

[0017] According to some embodiments of the liver perfusion system, the liver perfusion system further comprises a first blocking member configured to block the third opening, and a second blocking member configured to block the sixth opening.

[0018] According to some embodiments of the liver perfusion system, the liver perfusion system further comprises an upper cover detachably connected to the organ basin, the upper cover being configured to cover the opening of the storage cavity.

[0019] According to some embodiments of the liver perfusion system, the organ basin comprises a body portion and a protruding portion, the body portion being provided with the storage cavity, and the protruding portion being connected to the body portion and protruding towards the upper cover, the upper cover being provided with a positioning hole, and the protruding portion being arranged in the positioning hole.

[0020] According to some embodiments of the sterile detection method of the second aspect of the present application, the sterile detection method is used for detecting the liver perfusion system of any one of the first aspect of the embodiments, and the sterile detection method comprises the following steps:

[0021] The detection device is respectively communicated with the third opening and the sixth opening:

[0022] The buffer solution is poured into the organ basin, and then the buffer solution is circulated and flowed;

[0023] The buffer solution after multiple circulation flows is detected for sterility.

[0024] According to the sterile detection method of the embodiments of the present application, at least the following beneficial effects are achieved: through the detection device communicated with the third opening and the sixth opening, and through the buffer solution circulated and flowed in the liver perfusion system, and finally the buffer solution after flowing is detected for sterility, if the buffer solution is detected for bacteria, it can be determined that the liver perfusion system is bacteria, and the product is unqualified, otherwise, if the buffer solution is detected for sterility, it can be determined that the liver perfusion system is sterile, and the product is qualified, in this way, the sterile detection method can detect whether the liver perfusion system is sterile.

[0025] According to the detection device of the third aspect of the embodiments of the present application, the detection device is used for detecting the liver perfusion system of any one of the first aspect of the embodiments, and the detection device comprises:

[0026] The pipeline tool comprises an input pipe and an output pipe which are in communication with each other, the input pipe is in communication with the third opening and the sixth opening respectively, and one end of the output pipe is in communication with the organ basin;

[0027] A peristaltic pump is connected to the output pipe, and the peristaltic pump is used to drive the buffer solution to flow in the pipeline tool.

[0028] The detection device has at least the following beneficial effects: the third opening and the sixth opening are communicated through the pipeline tool, and one end of the output pipe is communicated with the organ basin, so that the buffer solution can circulate and flow, then the buffer solution circulates and flows in the liver perfusion system through the peristaltic pump, and finally the buffer solution after flowing is subjected to sterile detection, if the detection result of the buffer solution is bacteria, it can be determined that the liver perfusion system is bacteria, and the product is unqualified, otherwise, if the detection result of the buffer solution is sterile, it can be determined that the liver perfusion system is sterile, and the product is qualified, so that the detection device can detect whether the liver perfusion system is sterile.

[0029] According to some embodiments of the present application, the input pipe comprises a first section and a second section, the first section is in communication with the third opening, and the second section is in communication with the sixth opening, the pipeline tool further comprises a third joint, the third joint comprises a seventh opening, an eighth opening and a ninth opening which are in communication with each other, the seventh opening is in communication with the first section, the eighth opening is in communication with the second section, and the ninth opening is in communication with the output pipe.

[0030] Additional aspects and advantages of the present application will be made apparent from the following description. BRIEF DESCRIPTION OF DRAWINGS

[0031] The present application will be further described below in conjunction with the drawings and embodiments, wherein:

[0032] Fig. 1 is a schematic view of a liver perfusion system according to some embodiments of the present application;

[0033] Fig. 2 is an enlarged schematic view of A in Fig. 1;

[0034] Fig. 3 is a flowchart of a sterile detection method according to some embodiments of the present application;

[0035] Fig. 4 is a schematic view of a liver perfusion system and a detection device according to a first embodiment of the present application;

[0036] Fig. 5 is a schematic view of a detection device according to some embodiments of the present application;

[0037] Fig. 6 is a schematic view of a liver perfusion system and a detection device according to a second embodiment of the present application.

[0038] Reference signs: liver perfusion system 10, organ basin 100, body part 110, protruding part 120, first circulation line 200, first output tube 210, first perfusion tube 220, second circulation line 300, second output tube 310, second perfusion tube 320, first tube joint 400, first opening 410, second opening 420, third opening 430, second tube joint 500, fourth opening 510, fifth opening 520, sixth opening 530, first oxygenator 600, second oxygenator 700, first filter plug 800, second filter plug 900, upper cover 1000; detection device 20, tubing tool 30, input tube 31, first section 32, second section 33, output tube 34, third joint 35, seventh opening 36, eighth opening 37, ninth opening 38, peristaltic pump 40. DETAILED DESCRIPTION

[0039] The embodiments of the present application are described below in detail with reference to the accompanying drawings. In the drawings, the same or similar components are denoted by the same or similar reference numerals throughout. The embodiments described below are examples for explaining the present application and are not intended to limit the present application.

[0040] In the description of the present application, if the orientation description such as up, down, front, back, left, right, and the like is referred to, the orientation or positional relationship shown in the drawings is based on the orientation or positional relationship, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation of the present application.

[0041] In the description of the present application, if the meaning of several is more than one, the meaning of multiple is more than two, greater than, less than, more than, and the like are understood as not including the number, above, below, and the like are understood as including the number. If the first, second, and the like are described, they are only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or the order of technical features indicated.

[0042] In the description of the present application, unless otherwise explicitly limited, the words such as setting, installing, connecting, and the like should be broadly understood, and the person skilled in the art can reasonably determine the specific meaning of the above words in the present application in combination with the specific content of the technical solution.

[0043] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "exemplary embodiment", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the exemplary description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any appropriate manner in one or more embodiments or examples.

[0044] Please refer to FIG. 1, FIG. 2 and FIG. 4, in some embodiments, the liver perfusion system 10 comprises an organ basin 100, a first circulation pipeline 200, a second circulation pipeline 300, a first pipe joint 400 and a second pipe joint 500. The organ basin 100 has a storage cavity for accommodating an organ. For example, the storage cavity can accommodate a liver, so that the transplanted liver can be placed in the storage cavity to keep active. After the liver is placed in the organ basin 100, the first circulation pipeline 200 and the second circulation pipeline 300 are connected to the hepatic artery and the portal vein of the liver respectively, so as to supply blood and nutrients to the liver. In the present application, blood, nutrients and the like are collectively referred to as perfusion fluid.

[0045] The first circulation pipeline 200 comprises a first output pipe 210 and a first perfusion pipe 220. One end of the first output pipe 210 is in communication with the organ basin 100, and the other end of the first output pipe 210 is in communication with the first perfusion pipe 220. The first output pipe 210 is located outside the organ basin 100, and the first perfusion pipe 220 is located in the organ basin 100. After the first perfusion pipe 220 is fixed in the storage cavity, the first perfusion pipe 220 can be conveniently connected to the liver, such as the first perfusion pipe 220 and the hepatic artery of the liver. The first output pipe 210 is used for the perfusion fluid to flow from the organ basin 100, and the first perfusion pipe 220 is used for the perfusion fluid to flow from the first output pipe 210. In this way, after the liver is placed in the storage cavity, the first circulation pipeline 200 can circulate the perfusion fluid in the liver to keep the liver active.

[0046] The second circulation pipeline 300 comprises a second output pipeline 310 and a second perfusion pipeline 320. One end of the second output pipeline 310 is communicated with the organ basin 100, and the other end of the second output pipeline 310 is communicated with the second perfusion pipeline 320. The second output pipeline 310 is located outside the organ basin 100, and the second perfusion pipeline 320 is located in the organ basin 100. After the second perfusion pipeline 320 is fixed in the storage cavity, the second perfusion pipeline 320 can be conveniently connected with the liver, for example, the second perfusion pipeline 320 is connected with the portal vein of the liver. The second output pipeline 310 is used for flowing the perfusion liquid from the organ basin 100, and the second perfusion pipeline 320 is used for flowing the perfusion liquid from the second output pipeline 310. In this way, after the liver is placed in the storage cavity, the second circulation pipeline 300 can make the perfusion liquid circulate in the liver, so that the liver remains active.

[0047] The first pipe joint 400 comprises a first opening 410, a second opening 420 and a third opening 430 which are communicated with each other. The first opening 410 is communicated with the first output pipeline 210, the second opening 420 is communicated with the first perfusion pipeline 220, and the third opening 430 is used for being communicated with the detection device 20. The second pipe joint 500 comprises a fourth opening 510, a fifth opening 520 and a sixth opening 530 which are communicated with each other. The fourth opening 510 is communicated with the second output pipeline 310, the fifth opening 520 is communicated with the second perfusion pipeline 320, and the sixth opening 530 is used for being communicated with the detection device 20. Specifically, the detection device 20 is communicated with the third opening 430 and the sixth opening 530 respectively, so that the buffer solution can be placed in the storage cavity of the organ basin 100, and then the buffer solution is circulated. At this time, the buffer solution can pass through the storage cavity, the first output pipeline 210, the first perfusion pipeline 220, the second output pipeline 310 and the second perfusion pipeline 320, and the buffer solution can be detected whether it is sterile after multiple circulation. If the buffer solution is detected as having bacteria, it can be determined that the liver perfusion system 10 has bacteria, and the product is unqualified. On the contrary, if the buffer solution is detected as being sterile, it can be determined that the liver perfusion system 10 is sterile, and the product is qualified. Specifically, since the third opening 430 and the sixth opening 530 of the liver perfusion system 10 in the present application can be communicated with the detection device 20, when the liver perfusion system 10 is detected for sterility, the third opening 430 and the sixth opening 530 can be communicated with the detection device 20 for detection. In this way, the liver perfusion system 10 can facilitate the staff to perform the sterility detection.

[0048] It should be further noted that the liver perfusion system 10 needs to be sterile after processing. Since the liver perfusion system 10 is provided with a circulation pipeline to circulate the perfusion fluid in the liver, the volume of the liver perfusion system 10 is large and the devices are many, and it is difficult to perform a sterile check on multiple devices. The first pipe joint 400 and the second pipe joint 500 are connected to the detection device 20 respectively, so that the buffer solution can circulate in the liver perfusion system 10. It is conceivable that if there are bacteria in the liver perfusion system 10, the bacteria will come into contact with the buffer solution when the buffer solution circulates in the liver perfusion system 10. By detecting the buffer solution, it can be determined whether the liver perfusion system 10 is sterile. The buffer solution can be a sodium chloride-peptone buffer solution with a pH of 7.0.

[0049] It should be further noted that the first perfusion pipe 220 and the second perfusion pipe 320 are connected to the liver, so the diameters of the first perfusion pipe 220 and the second perfusion pipe 320 are smaller than the diameters of the first output pipe 210 and the second output pipe 310. If the liver perfusion system 10 does not have the first pipe joint 400 and the second pipe joint 500, and is directly connected to the detection device 20 through the first perfusion pipe 220 and the second perfusion pipe 320, then the diameters of the first perfusion pipe 220 and the second perfusion pipe 320 need to match the pipe diameter of the detection device 20. However, the pipe diameter of the detection device 20 is generally larger than the diameter of the first perfusion pipe 220, because this can facilitate the flow of the buffer solution and improve the flow rate of the buffer solution and improve the detection speed. Therefore, by providing the first pipe joint 400 and the second pipe joint 500, the liver perfusion system 10 can be easily matched with the liver and the detection device 20.

[0050] Further, please refer to FIG. 1, in some embodiments, the liver perfusion system 10 further comprises a first oxygenator 600 and a second oxygenator 700, the first oxygenator 600 is in communication with the first circulation pipeline 200, and the second oxygenator 700 is in communication with the second circulation pipeline 300. Specifically, the first oxygenator 600 can be in communication with the first output pipe 210, and the first oxygenator 600 can remove carbon dioxide in the perfusion fluid and oxygenate blood and oxygen, so that the first circulation pipeline 200 can provide oxygen to the liver. The second oxygenator 700 can be in communication with the second output pipe 310, and the second oxygenator 700 can remove carbon dioxide in the perfusion fluid and oxygenate blood and oxygen, so that the second circulation pipeline 300 can provide oxygen to the liver.

[0051] It should be noted that, in the sterile detection, the buffer solution can also pass through the first oxygenator 600 and the second oxygenator 700, so as to detect whether the first oxygenator 600 and the second oxygenator 700 are sterile.

[0052] Further, referring to FIG. 1, in some embodiments, the liver perfusion system 10 further comprises a first filter 800 and a second filter 900, the first filter 800 being in communication with the first circulation pipeline 200, and the second filter 900 being in communication with the second circulation pipeline 300. Specifically, the first filter 800 and the first circulation pipeline 200 can be in communication in that the first filter 800 and the first output pipeline 210 are in communication. The second filter 900 and the second circulation pipeline 300 can be in communication in that the second filter 900 and the second output pipeline 310 are in communication. The first filter 800 and the second filter 900 can be used to filter various microthrombi generated in the extracorporeal circulation of the liver, which can prevent the embolism of liver microvessels caused by various microthrombi such as thrombus or air embolus, and effectively improve the blood perfusion of human microvessels.

[0053] It should be noted that, in the sterile detection, the buffer solution can also pass through the first filter 800 and the second filter 900, so as to detect whether the first filter 800 and the second filter 900 are sterile.

[0054] Further, referring to FIG. 1, in some embodiments, the liver perfusion system 10 further comprises a first driving member and a second driving member, the first driving member being in communication with the first circulation pipeline 200, and the second driving member being in communication with the second circulation pipeline 300, the first driving member being used to drive the perfusion solution to flow in the first circulation pipeline 200, and the second driving member being used to drive the perfusion solution to flow in the second circulation pipeline 300. The first driving member and the second driving member can both be centrifugal pumps, which can make the perfusion solution flow, so as to realize the continuous circulation of the perfusion solution and maintain the activity of the liver.

[0055] Further, in some embodiments, the liver perfusion system 10 further comprises a first blocking member for blocking the third opening 430, and the liver perfusion system 10 further comprises a second blocking member for blocking the sixth opening 530. Specifically, after the liver perfusion system 10 is detected to be sterile, if the liver perfusion system 10 is qualified, in order to make the perfusion liquid flow out of the first output tube 210 in the subsequent use, instead of flowing out of the third opening 430, the third opening 430 can be blocked by the first blocking member, so as to ensure that the perfusion liquid flows out of the first perfusion tube 220, instead of flowing out of the third opening 430. Similarly, in order to make the perfusion liquid flow out of the second output tube 310 in the subsequent use, instead of flowing out of the sixth opening 530, the sixth opening 530 can be blocked by the second blocking member, so as to ensure that the perfusion liquid flows out of the second perfusion tube 320, instead of flowing out of the sixth opening 530.

[0056] Further, referring to FIGS. 1 and 6, in some embodiments, the liver perfusion system 10 further comprises a cover 1000, which is detachably connected to the organ basin 100, and the cover 1000 is used for covering the opening of the storage cavity. When the cover 1000 is connected to the organ basin 100, the cover 1000 can prevent dust from entering the organ basin 100.

[0057] Further, after the cover 1000 is placed on the organ basin 100, the cover 1000 can be moved and thus fall off the organ basin 100. Therefore, in order to solve this problem, the organ basin 100 can be provided with the protruding part 120. Specifically, referring to FIGS. 1 and 2, in some embodiments, the organ basin 100 comprises a body part 110 and a protruding part 120, the body part 110 is provided with a storage cavity, the protruding part 120 is connected to the body part 110 and protrudes towards the cover 1000, the cover 1000 is provided with a positioning hole, and the protruding part 120 is arranged in the positioning hole. In this way, when a worker places the cover 1000 on the organ basin 100, if the protruding part 120 does not touch the positioning hole, the cover 1000 cannot be placed on the organ basin 100, and the worker can know that the direction of the cover 1000 is incorrect, and the worker can adjust the direction of the cover 1000 so that the protruding part 120 is located in the positioning hole. In addition, after the cover 1000 is placed on the organ basin 100, the cover 1000 cannot be moved because the protruding part 120 is located in the positioning hole. Specifically, when someone touches the organ basin 100, the cover 1000 will not be impacted by external force and thus will not fall off.

[0058] The sterile detection method is described below. Referring to FIGS. 3 and 4, in some embodiments, the sterile detection method is used for detecting the sterility of the liver perfusion system 10 in any of the above embodiments, and the sterile detection method comprises the following steps:

[0059] S100, connecting the detection device 20 to the third opening 430 and the sixth opening 530 respectively:

[0060] S200, pouring the buffer into the organ basin 100, and then circulating the buffer;

[0061] S300, detecting whether the buffer after multiple circulation is sterile.

[0062] Specifically, after connecting the third opening 430 and the sixth opening 530 through the detection device 20, and circulating the buffer in the liver perfusion system 10, finally, the buffer after flowing is detected for sterility. If the buffer detection result is bacteria, it can be determined that the liver perfusion system 10 is bacteria, and the product is unqualified. On the contrary, if the buffer detection result is sterile, it can be determined that the liver perfusion system 10 is sterile, and the product is qualified. In this way, the sterile detection method can detect whether the liver perfusion system 10 is sterile.

[0063] The specific process of the sterile detection method is introduced again as follows. 1. Connect the first section 32 and the third opening 430 with sterile forceps, and connect the second section 33 and the sixth opening 530; 2. Connect the output tube 34 and the peristaltic pump 40, and then place one end of the output tube 34 in the storage cavity of the organ basin 100; 3. Pour about 1000 ml of pH 7.0 sodium chloride-protein peptone buffer into the organ basin 100 and cover the upper cover 1000; 4. Start driving the buffer circulation through the peristaltic pump 40, wherein the peristaltic condition is 500 ml / min, and the peristaltic time is 2 min; 5. Transfer the buffer after circulation to the bacteria collection instrument 6. First, start the bacteria collection instrument (about 90 rpm), and then start the peristaltic pump 40 (180 ml / min) to filter the buffer into 3 independent filter cups, respectively. After the washing liquid is completely filtered, the bottom of the filter cup is blocked with a matching plug. (The needle and the pipeline port are burned with an alcohol lamp); 7. Add culture medium: 7.1. Insert the disposable bacteria collector needle end into the saline bottle containing the thioglycolate fluid medium (FT) or the tryptone soya broth liquid medium (TSB), start the bacteria collection instrument for filtration, and filter the culture medium into the filter cup. At least one filter cup contains each culture medium. The extra one containing buffer is used as a positive control. Clamp the pipeline with a pipeline clamp, then cut the pipeline of the filter cup and insert it into the air filter hole, and seal the filter cup. (The outer surface of the bacteria collector needle is quickly burned with an alcohol lamp); 7.2. Positive control: inoculate not more than 100 cfu of Staphylococcus aureus bacterial suspension into the thioglycolate fluid medium (FT) containing 1 piece of sample; 7.3. Negative control: take 100 ml of thioglycolate fluid medium (FT) and 100 ml of tryptone soya broth liquid medium (TSB) as negative controls, respectively; 8. Culture and observation: culture the above-mentioned disposable sterile filter cup containing thioglycolate fluid medium (FT) at 30-35°C for 14 days, and culture the disposable bacteria collector containing tryptone soya broth liquid medium (TSB) at 20-25°C for 14 days. During the culture period, observe and record whether there is bacterial growth. If there is bacterial growth, it is determined that there is bacteria in the liver perfusion system 10, and the liver perfusion system 10 is unqualified. If there is no bacterial growth, it is determined that there is no bacteria in the liver perfusion system 10, and the liver perfusion system 10 is qualified.

[0064] The detection device 20 is introduced below, please refer to FIG. 4 and FIG. 5, in some embodiments, the detection device 20 is used for detecting the liver perfusion system 10, the detection device 20 comprises a pipeline tool 30 and a peristaltic pump 40. The pipeline tool 30 comprises an input pipe 31 and an output pipe 34 which are communicated with each other, the input pipe 31 is communicated with the third opening 430 and the sixth opening 530 respectively, one end of the output pipe 34 is communicated with the organ basin 100. The peristaltic pump 40 is connected to the output pipe 34, the peristaltic pump 40 is used for driving the buffer solution to flow in the pipeline tool 30. Specifically, by connecting the third opening 430 and the sixth opening 530 through the pipeline tool 30, and connecting one end of the output pipe 34 to the organ basin 100, the buffer solution can be circulated, then the buffer solution is circulated in the liver perfusion system 10 by the peristaltic pump 40, finally the buffer solution after flowing is detected for sterility, if the buffer solution is detected to be sterile, then it can be determined that the liver perfusion system 10 is sterile, and the product is qualified, otherwise, if the buffer solution is detected to be unsterile, then it can be determined that the liver perfusion system 10 is unsterile, and the product is unqualified, in this way, the detection device 20 can detect whether the liver perfusion system 10 is sterile.

[0065] Further, please refer to FIG. 5, in some embodiments, the input pipe 31 comprises a first section 32 and a second section 33, the first section 32 is communicated with the third opening 430, the second section 33 is communicated with the sixth opening 530, the pipeline tool 30 further comprises a third joint 35, the third joint 35 comprises a seventh opening 36, an eighth opening 37 and a ninth opening 38 which are communicated with each other, the seventh opening 36 is communicated with the first section 32, the eighth opening 37 is communicated with the second section 33, the ninth opening 38 is communicated with the output pipe 34. In this way, by connecting the first section 32 and the second section 33 to the output pipe 34 through the third joint 35, the detection device 20 can drive the buffer solution to flow in the liver perfusion system 10 through one output pipe 34 and one peristaltic pump 40. If there is no third joint 35, then two input pipes 31, two output pipes 34 and two peristaltic pumps 40 are needed, which will result in high cost.

[0066] The embodiments of the present application are described in detail above with reference to the drawings, but the present application is not limited to the above embodiments, within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the purpose of the present application. In addition, the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

Claims

1. A liver perfusion system, characterized by, The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system.

2. The liver perfusion system of claim 1, wherein, The application relates to a liver perfusion system.

3. The liver perfusion system of claim 1, wherein, The application relates to a liver perfusion system.

4. The liver perfusion system of claim 1, wherein, The application relates to a liver perfusion system.

5. The liver perfusion system of claim 1, wherein, The application relates to a liver perfusion system.

6. The liver perfusion system of claim 1, wherein, The application relates to a liver perfusion system.

7. The liver perfusion system of claim 6, wherein, The application relates to a liver perfusion system.

8. A method for sterile detection of a liver perfusion system according to any one of claims 1 to 7, characterized in that The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. 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The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion system. The application relates to a liver perfusion 9. A detection device for detecting the liver perfusion system according to any one of claims 1 to 7, characterized in that, The detection device comprises: A pipeline tool comprising an input pipe and an output pipe in communication with each other, the input pipe being in communication with the third opening and the sixth opening respectively, one end of the output pipe being in communication with the organ basin; A peristaltic pump connected to the output pipe, the peristaltic pump being used to drive the buffer solution in the pipeline tool to flow.

10. The detection device of claim 9, wherein, The input pipe comprises a first section and a second section, the first section being in communication with the third opening, and the second section being in communication with the sixth opening, the pipeline tool further comprising a third joint, the third joint comprising a seventh opening, an eighth opening and a ninth opening in communication with each other, the seventh opening being in communication with the first section, the eighth opening being in communication with the second section, and the ninth opening being in communication with the output pipe.

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