Vertical platform for culturing and observing tissue or cells, and monitoring system thereof

Abstract

The present invention relates to a vertical platform for culturing and observing tissue or cells, and a monitoring system thereof, the platform comprising: a partition wall for partitioning into a culture space part which has a first passage part for injecting tissue or cells for culture, and a culture medium storage space part which has a second passage part; a transparent platform body part which has, formed in the partition wall, at least one culture medium transfer passage for supplying a culture medium of the culture medium storage space part to the culture space part; and a transparent plate member coupled to the partition wall on one side surface of the platform body part so as to seal the open culture space part and culture medium storage space part.

Description

A vertical platform for culturing and observing tissues or cells and a monitoring system thereof.

[0001] The present invention relates to a platform for cell culture, and more specifically, to a vertical platform for culturing and observing tissues or cells, which is capable of improving the separability of cultured tissues and securing an air-liquid interface between cultured tissues or cells, and which has a highly transparent hydrophobic observation section, and a monitoring system thereof.

[0002] In general, a typical cell culture vessel for cell culture is composed of a culture dish and a cover, and if necessary, a transparent material such as a cover glass or slide glass is added, and has a structure with a wide bottom plane for culture.

[0003] In the case of skin cells or skin tissues, observation of cells or tissues is important because they have a growth mechanism that forms a vertical layered structure during the process of cell division and differentiation. However, side observation of cells or tissues is practically impossible with conventional cell culture vessels.

[0004] Furthermore, tissues that have completed differentiation and growth must be separated from the culture vessel (i.e., glass) for observation. However, structural deformation tends to occur during this separation process. If problems such as membrane cracking or cell detachment occur during this process, it is difficult to obtain normal experimental results.

[0005] In addition, it is difficult to replace the culture medium in conventional culture vessels, and it is difficult to secure an air-liquid interface because the space where cells and tissues grow, layer, and organize is narrow.

[0006] Considering these points, Korean Patent Publication No. 2022-0092275 discloses a cell culture plate stacking arrangement. The disclosed stacking arrangement has a structure in which cell culture plates are stacked vertically to enable cell culture through interaction between different types of cells.

[0007] Korean Patent Publication No. 2014-0040704 discloses a cell culture system, and Korean Patent Publication No. 2020-021108 discloses a culture method, which has a configuration in which side walls are installed in a vertical direction with respect to the culture bottom surface of the culture vessel.

[0008] The conventional culture container described above does not allow for easy side observation of cultured cells and it is difficult to secure a gas-liquid interface.

[0009] A cell culture unit is disclosed in Korean Patent No. 10-1215657.

[0010] Meanwhile, to overcome the limitations of two-dimensional cell culture, active research is being conducted on three-dimensional cell culture. Three-dimensional cell culture using microwell arrays offers the advantage of simulating the in vivo three-dimensional cell environment. However, conventional microwell arrays are highly complex to manufacture and expensive.

[0011] Conventional microwell arrays have the problem of causing cell spheroids to be lost between the well spaces during cell seeding and causing cells to be detached from the well spaces during cell culture.

[0012] A microfluidic multi-well based cell culture test device is disclosed in Korean Patent No. 10-1446526.

[0013] The present invention is intended to solve the above-described problems, and provides a vertical platform for culturing and observing tissues or cells, and a monitoring system thereof, which enables observation of structural and physiological characteristics, including aspects, that appear during the culturing process of cells, living tissues, organoids, artificial skin, and other biological cultures, and enables securing of a gas-liquid interface, thereby activating the culturing of cells and tissues.

[0014] Another object of the present invention is to provide a vertical platform for culturing tissues or cells, which can reduce the amount of culture medium required for culturing cells and provide electrical stimulation to the cultured cells, and a monitoring system thereof.

[0015] In order to achieve the above purpose, the vertical platform for tissue or cell culture and observation of the present invention comprises a partition wall for dividing a culture space section in which a first passage section for injecting tissue or cells for culture is formed and a culture solution storage space section in which a second passage section is formed, and a transparent platform body section in which at least one culture solution transfer passage for supplying culture solution from the culture solution storage space section to the culture space section is formed in the partition wall,

[0016] It is characterized by having a transparent plate member that seals the open culture space and the culture solution storage space by being combined with a partition wall on one side of the platform body.

[0017] In the present invention, the platform body portion is made of one selected from among polydimethylsiloxane (PDMS), polyurethane, and polycarbonate, which are organic silicon compounds.

[0018] The above transparent plate member is coated with a hydrophobic polymer to form a hydrophobic coating layer.

[0019] The above partition walls have side partition walls that are parallel to each other and spaced apart at a predetermined interval, and a bottom partition wall that connects the lower part of the side partition wall to partition the culture space.

[0020] An extremely hydrophobic surface layer is formed on the inner surface of the side wall exposed to the above-mentioned basalt space to form a gas-liquid interface.

[0021] Alternatively, a vertical platform for culturing and observing tissues or cells to achieve the above purpose comprises a culture medium storage space formed by retracting one side thereof, a culture space partitioned by a partition wall within the culture space partition and having at least three sides exposed by the culture medium storage space partition, a platform body portion having a first passage portion connected to the culture space partitioned by the partition wall, a second passage portion formed on an upper side of the culture medium storage space portion, and at least one culture medium supply passage formed to supply culture medium to the culture space portion from the upper surface side of the partition wall partitioning the culture space portion;

[0022] A transparent plate member is provided that seals the open culture space and the culture solution storage space by being joined or adhered to the partition wall on one side of the platform body.

[0023] A vertical platform monitoring system for culturing and observing tissues or cells to achieve the above purpose comprises a platform having a transparent platform body, a culture space having a first passage formed on one side of the platform body, and a culture solution storage space having a second passage formed thereon, a partition wall having at least one culture solution supply passage formed therein for supplying culture solution from the culture solution storage space to the culture space, and a transparent plate member coupled to the platform body to seal the culture space and the culture solution storage space divided by the partition wall.

[0024] On the side corresponding to the above shooting unit, a lighting unit is installed to irradiate light to the platform being shot,

[0025] It has a control server that controls the above-mentioned shooting unit, lighting unit, and measuring unit, and includes a monitor for displaying the captured image.

[0026] In the present invention, a first electrode is embedded in the platform body and an end is exposed to the culture space, and a second electrode is provided whose end is exposed to the culture solution storage space.

[0027] The electrical resistance between the culture space and the culture solution storage space can be measured in real time using the first and second electrodes, and the TEEL measurement unit is further provided to provide an appropriate stimulus to the cultured cells through the first and second electrodes.

[0028] The above control server includes a collection unit that collects information captured by a shooting unit, a diagnosis unit that diagnoses the cell culture status using the collected cell culture information, and a storage unit for storing the collection information collected by the collection unit and the information diagnosed by the diagnosis unit.

[0029] The above TEEL measurement unit is controlled to control the culture state of tissues and cells, and includes a control unit for controlling the photographing unit.

[0030] The vertical platform for culturing and observing tissues or cells according to the present invention is vertically erected, enabling side observation of structural and physiological characteristics that appear during the culturing of cells, living tissues, organoids, artificial skin, artificial organs, multi-organ 3D biomimetic tissues, and other biological cultures.

[0031] In addition, the monitoring system of the vertical platform for culturing tissues or cells according to the present invention can continuously monitor the tissue and culture status of cells, and can evaluate the integrity of a cell monolayer by measuring electrical resistance.

[0032] A vertical platform for tissue or cell culture and observation is erected vertically to enable observation of structural and physiological characteristics that appear during the culturing of cells, living tissues, organoids, artificial skin, and other biological cultures.

[0033] Figure 1 is an exploded perspective view of a vertical platform for tissue or cell culture and observation according to the present invention;

[0034] Figure 2 is a partially cutaway perspective view of a vertical platform for tissue or cell culture and observation according to the present invention;

[0035] Figure 3 is a front view of a vertical platform for culturing and observing tissues or cells according to the present invention, showing the state in which cells are cultured.

[0036] FIG. 4 is a partially cutaway perspective view illustrating another embodiment of a vertical platform for tissue or cell culture and observation according to the present invention;

[0037] FIG. 5 is an exploded perspective view illustrating another embodiment of a vertical platform for tissue or cell culture and observation according to the present invention.

[0038] FIG. 6 is a perspective view of the vertical platform for tissue or cell culture and observation shown in FIG. 5, and FIG. 7 is a perspective view showing the vertical platform for tissue or cell culture and observation according to the present invention installed on a stand.

[0039] Figure 8 is a perspective view showing a platform for cell culture mounted in a sterile case.

[0040] Figure 9 is a front view showing a platform for cell culture wrapped in a sterile packaging pouch.

[0041] Figure 10 is a perspective view illustrating a monitoring system of a vertical platform for culturing tissues or cells.

[0042] The present invention relates to a vertical platform for culturing and observing tissues or cells and a monitoring system thereof, examples of which are shown in FIGS. 1 to 9 and FIG. 10.

[0043] Referring to the drawings, the vertical platform (10) for culturing and observing tissues or cells according to the present invention comprises a transparent platform body (20) and a transparent plate member (30) that is combined therewith to form a culture space (21) for culturing cells and a culture solution storage space (23) for supplying culture solution to the culture space (21). The culture space (21) is surrounded by the culture solution storage space (23), and at least three sides can be surrounded.

[0044] The above platform body part (20) is formed in a plate shape and has a culture space part (21) and a culture solution storage space part (23) introduced from one side thereof and a partition wall (25). The partition wall (25) can be formed by introducing the culture space part (21) and the culture solution storage space part (23) surrounding the culture solution space part (21) from one side of the platform body part (20).

[0045] The partition wall (25) that divides the culture space (21) and the culture solution storage space (23) is provided with a side partition wall (25a) that is spaced apart at a predetermined interval and is parallel to each other and a bottom partition wall (25b) that connects the lower portion of the side partition wall (25a). The upper portions of the side partition walls (25a) are open to form a first passage (25c) for cell injection.

[0046] Although not shown in the drawing, the first passage portion (25c) forms a connecting bulkhead portion connecting the upper portions of the side bulkhead portions (25a), and may be formed in this connecting bulkhead portion.

[0047] An extremely hydrophobic surface (26) of micro-nano structure is formed on the inner surfaces corresponding to each other of the side partition walls (25a), i.e., the inner surfaces that divide the culture space (21), thereby forming a gas-liquid interface between the cultured cell tissues. The extremely hydrophobic surface (26) can be formed by forming micro-nano irregularities on the inner surfaces of the partition walls (25) facing each other so that air can remain therein.

[0048] A culture medium transfer passage (27) for supplying the culture medium stored in the culture medium storage space (23) to the culture space (21) is formed in the lower part of the side partition wall (25a) and the bottom partition wall (25b).

[0049] It is preferable that the culture solution storage space (23) of the platform body (20) be formed in a structure that surrounds the culture solution space (21) with the partition wall (25) interposed therebetween. In addition, a second passage (23a) is formed on the upper side of the platform body (20), that is, in a position parallel to the first passage (25c), for supplying the culture solution to the culture solution storage space (23).

[0050] A joining protrusion (28) may be further provided at the edge of the transparent platform body part (20) or the edge of the transparent plate member to limit the joining position of the platform body part (20) and the transparent plate member (30).

[0051] The platform body portion configured as described above may be made of one selected from among polydimethylsiloxane (PDMS), polyurethane, and polycarbonate, which are organic silicon compounds.

[0052] The above transparent plate member (30) is formed to have a size substantially the same as or smaller than that of the platform body part (20), and is adhered to the platform body part (20) by an adhesive or a bonding means to seal the culture space part (21) and the culture solution storage space part (23) except for the first and second passage parts (25c) (23a).

[0053] A hydrophobic coating layer coated with a hydrophobic polymer is formed on the inner surface of the above transparent plate member (30) to facilitate separation of cultured cells or tissues and to ensure transparency.

[0054] As shown in FIGS. 5 and 6, the coupling means (50) is configured to partition the culture space (21) and the culture solution storage space (23) by bringing the platform body part (20) and the transparent plate member (30) into close contact, and includes a body frame (51) supported by the platform body part (20), and coupling hooks (52) that extend from the body frame (51) and are coupled to the edge of the transparent plate member (30) to bring the transparent plate member (30) into close contact with the platform body part (20).

[0055] And the above-mentioned coupling means (50) may be installed between the platform body part (20) and the transparent plate member (30), that is, along the edge of the partition wall (25) and the culture medium space part (23), and a gasket (not shown) may be further provided to maintain airtightness.

[0056] The material of the above gasket may be made of rubber, silicone, foam elastomer, soft synthetic resin, etc.

[0057] The above-described coupling means is not limited to the above-described embodiment, and may be any structure capable of tightly fixing the transparent plate member (30) to the platform body portion (20). For example, it may be composed of a clip for tightly fixing the platform body portion and the transparent plate member, and a clamp for fastening.

[0058] In addition, a cover member (40) may be further provided on the upper part of the vertical platform (10) in which the flat body part (20) and the transparent plate member (30) are combined to prevent the culture solution from evaporating through the first and second passages (25c) (23c).

[0059] And the vertical platform (10) for culturing and observing tissues or cells according to the present invention may further include a semi-permeable membrane support layer (60) on the lower side of the culture space (21) partitioned by a partition wall (25), and may further include an electric stimulation means (70) for providing electric stimulation to the cultured tissues or cells as shown in FIGS. 4 and 5.

[0060] The semipermeable membrane support layer (60) above can support cells or tissues cultured in the culture space (21). The semipermeable membrane support layer (60) is made of a semipermeable membrane, an extracellular matrix, a hydrogel, or a combination of multiple thereof, and can prevent cells cultured in the culture space (21) from falling while allowing selective material exchange with the culture medium, or only providing signaling substances that promote cell division and cell differentiation in the culture medium.

[0061] The semipermeable membrane is made of one or a combination of PES, PS, PP, PET, cellulose, nitrocellulose, and cellulose acetate as a scaffold material, and the extracellular matrix is ​​made of one or a combination of collagen, gelatin, hyaluronic acid, and Matrigel, and the hydrogel may be made of one or a combination of collagen, gelatin, hyaluronic acid, Matrigel, GelMA, ColMA, fibrin, cellulose, and hemicellulose, agarose, and alginic acid.

[0062] The above extracellular matrix or hydrogel may include cross-linking to impart physical properties. For cross-linking, a methacrylated substance or metal ion, such as magnesium ion or calcium ion, may be used, and UV curing or thermal curing may be used for the cross-linking reaction.

[0063] The above-mentioned electric stimulation means (70) can apply an electric stimulus to cultured cells or tissues, and the end of the first electrode (71) is installed so that it can be exposed to the culture space (21), and the end of the second electrode (75) is installed so that it can be exposed to the culture solution storage space (23). The first and second electrodes are embedded in the platform body (20).

[0064] The above-described electrical stimulation means (70) can be used as an electrode for measuring transepithelial electrical resistance (TEER) to measure the electrical resistance between the culture space (21) and the culture solution storage space (23) in real time to measure the physical robustness or durability of cells or tissues being cultured as described above.

[0065] Meanwhile, in another embodiment, the vertical platform for culturing and observing tissues or cells according to the present invention may have a plurality of culture spaces (21) partitioned by partition walls (25) as described above inside the culture solution storage space (23). In this case, the first passage (25c) formed in the culture space (21) and the second passage (23c) for supplying culture solution to the culture solution storage space (23) are formed in the same direction, i.e., on the upper side.

[0066] As shown in FIGS. 7 and 8, the observation platforms can be used in a state where they are sequentially supported on a stand, and as shown in FIG. 9, they can be supported inside a sterilized pouch.

[0067] Meanwhile, an embodiment of a monitoring system (100) capable of continuously photographing and monitoring the vertical layer structure and real-time observation during the cultivation of tissues or cells using a vertical platform for cell culture configured as described above is shown in FIG. 10.

[0068] Referring to the drawing, a monitoring system (100) for monitoring a vertical platform includes a frame (101) and a support unit (105) installed on the frame (101) to support the platform (10).

[0069] And it includes a photographing unit (110) for photographing cells or tissues cultured in a culture space (21) of a platform (10) supported by a support unit (105) and installed on one side of the frame (101). And it includes a TEER (transepithelial electrical resistance) measuring unit (120) for adjusting a culture environment by giving electrical stimulation to cells cultured in the culture space and for evaluating the integrity of a monolayer by measuring the resistance value of cells, and an illumination unit (130) for irradiating light to the platform (10) to be photographed is installed on a side corresponding to the photographing unit (110).

[0070] The above-mentioned shooting unit (110) is for monitoring the platform (10) installed on the frame (101) and shooting images, and includes a camera module including a zoom function and a microscope for magnified shooting.

[0071] The above-mentioned shooting unit (110) is equipped with an XYZ-axis moving table (not shown) for moving a camera (111) including a microscope in the X, Y, and Z-axis directions, a monitor (112) for displaying an image captured by the camera (111), and a control server (113). The camera (111) may be formed of a camera module using a solid-state imaging device.

[0072] A camera (111) controlled by a control server (113) can capture images enlarged by a microscope by time unit.

[0073] The above control server (113) may include, although not shown in the drawing, a collection unit for collecting information captured by a camera (111), a diagnosis unit for diagnosing the culture status of cells using the collected cell culture information, and a storage unit for storing the collected information collected by the collection unit and the information diagnosed by the diagnosis unit. In addition, the above control server (113) may include a culture environment control unit for controlling the culture ecological environment of tissues and cells by controlling the TEEL measurement unit (120).

[0074] Meanwhile, the TEEL measurement unit (120) is embedded in the platform body (20) as described above, and can measure the electrical resistance between the culture space (21) and the culture solution storage space (23) to measure the physical robustness or durability of cells or tissues being cultured in real time by using the first electrode (71) having an end exposed to the culture space (21) and the second electrode (72) having an end exposed to the culture solution storage space (23). That is, the first and second electrodes (71) and (72) can be used as electrodes for measuring the electrical resistance of the epithelium. In addition, the TEEL measurement unit (120) can provide an electrical stimulus to cells being cultured in order to diversify the culture environment.

[0075] The above lighting unit (130) is equipped with a light source for irradiating light onto the platform. It may further include a fluorescent light source for emitting light of a specific wavelength to induce a fluorescent reaction in a fluorescently stained cultured cell or tissue sample and for observing the same.

[0076] The above lighting unit (130) may further include a condenser lens unit for improving the quality of the illumination light by focusing the illumination light irradiated from a light source or a fluorescent light source onto cells or tissues cultured on the platform (10) to have a uniform light density, an aperture diaphragm for controlling the spot size of the illumination light passing through the condenser lens unit to control contrast and resolution, and a field diaphragm for setting the irradiation range, i.e., the area to which light is irradiated, to reduce unnecessary scattered light and to increase the clarity of the observation field.

[0077] The operation of the tissue and cell culture using the vertical platform (10) for culturing and observing tissues or cells according to the present invention configured as described above and the monitoring system for observing the cultured cells will be described as follows.

[0078] In order to culture tissues or cells using the platform (10), the culture solution is injected into the culture solution storage space (23) through the second passage (23a), and the tissues or cells to be cultured are injected through the first passage (25c) of the culture space (21).

[0079] The cells injected into the above culture space (21) are cultured by supplying the culture solution injected into the culture storage space (23) to the culture space (21) through the culture solution transfer passage (27) formed in the partition wall (25).

[0080] This cultivation process can be observed in real time through the monitoring system described below.

[0081] When culturing skin cells or skin tissue, it is easy to observe the side of the cells or tissue because they have a mechanism that forms a vertical layered structure during the process of cell division and differentiation.

[0082] In addition, micro-nanoparticles are formed on the inner surface of the side partition wall (25a) forming the partition wall (25) for partitioning the culture space (21), that is, the inner surface exposed toward the culture space (21), so that an extremely hydrophobic surface (26) is formed, thereby forming an air-liquid interface between the surface and the culture medium of the culture space (21). Accordingly, by expanding the air-liquid interface within the culture space (21), oxygen can be smoothly supplied to tissues or cells, thereby activating the growth of tissues or cells. In addition, a hydrophobic surface coating layer is formed on the inner surface of the transparent plate member (30), so that the separation of cultured cells is easy and high transparency can be secured.

[0083] As described above, the process of culturing cells in the culture space (21) of the planform (10) according to the present invention can be continuously observed using the monitoring unit (100), and the resistance of the cultured tissue or cells can be measured using the TEEL measurement unit (70) to confirm the integrity of the cell monolayer, and the culture conditions can be diversified by providing electrical stimulation. In addition, the cultured tissue or cells can be continuously observed and images can be obtained using the photographing unit (110).

[0084] Monitoring using a photographing unit (100) can continuously observe the mechanism, cells or tissues that form a vertical layered structure during the process of cell division and differentiation when culturing skin cells or skin tissues.

[0085] As described above, images obtained through continuous tissue or cell culture using the monitoring unit are stored in the monitoring server, and the cell culture status can be diagnosed through the images.

[0086] As described above, the vertical platform for culturing tissues or cells according to the present invention and its monitoring system enable real-time monitoring of cultured tissues and cells by using a platform that can expand the gas-liquid interface within the culture space and continuously supply culture solution to the cultured tissues and cells, thereby enabling high-quality cell culture.

[0087] While the present invention has been described with reference to the embodiments illustrated in the drawings, these are merely exemplary, and those skilled in the art will appreciate that various modifications and equivalent alternative embodiments are possible. Therefore, the true scope of technical protection of the present invention should be determined by the technical spirit of the appended claims.

Claims

1. A transparent platform body portion having a partition wall for dividing a culture space portion having a first passage portion for injecting tissues or cells for culture and a culture solution storage space portion having a second passage portion, and at least one culture solution transfer passage formed in the partition wall for supplying culture solution from the culture solution storage space portion to the culture space portion, A vertical platform for culturing and observing tissues or cells, characterized in that it has a transparent plate member that seals an open culture space and a culture solution storage space by being combined with a partition wall on one side of the platform body.

2. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that the platform body portion is made of one selected from among polydimethylsiloxane (PDMS: polydime-thylsiloxane), polyurethane, and polycarbonate, which are organic silicon compounds.

3. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that the transparent plate member is coated with a hydrophobic polymer to form a hydrophobic coating layer.

4. In claim 1, The above partition walls have side partition walls that are parallel to each other and spaced apart at a predetermined interval, and a bottom partition wall that connects the lower part of the side partition wall to partition the culture space. A vertical platform for culturing and observing tissues or cells, characterized in that an extremely hydrophobic surface layer is formed on the inner surface of the side partition exposed to the above-mentioned embryonic space to form a gas-liquid interface.

5. In claim 4, A vertical platform for culturing and observing tissues or cells, characterized in that the above-mentioned ultra-hydrophobic surface layer is formed with micro-nano irregularities so that the inner surface of the side walls can retain air.

6. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that the first passage connected to the culture space formed in the platform body and the second passage connected to the culture solution storage space are further provided with a cover member that prevents evaporation of the culture solution from the culture space and the culture solution storage space.

7. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that it further comprises a joining frame for joining the platform body and the transparent plate member.

8. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that one side of the platform body further includes a joining protrusion that defines a joining position of the platform body and the transparent plate member.

9. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that the platform body part and one side of the transparent plate part are further provided with an electrical stimulation means capable of applying electrical stimulation to cultured cells or tissues and measuring electrical resistance between the culture space part and the culture solution storage space part during the process of culturing cells or tissues.

10. In claim 9, A vertical platform for culturing and observing tissues or cells, characterized in that the above-mentioned electric stimulation means is embedded in a partition wall or a platform body and has a first electrode with an end exposed to a culture medium space and a second electrode with an end exposed to a culture medium storage space.

11. In claim 1, A vertical platform for culturing and observing tissues or cells, characterized in that a semi-permeable membrane support layer for supporting cultured cells or tissues is further provided on the lower side of the culture space partitioned by the above-mentioned partition wall.

12. In claim 1, The above semipermeable membrane support layer is composed of a semipermeable membrane, an extracellular matrix, a hydrogel, or a combination of multiple thereof, The above semi-permeable membrane is made of one of PES, PS, PP, PET, cellulose, nitrocellulose, and cellulose acetate as a scaffold material, or is made of a combination of multiple materials, The above extracellular matrix is ​​composed of one or a combination of multiple materials including collagen, gelatin, hyaluronic acid, and Matrigel. A vertical platform for culturing and observing tissues or cells, characterized in that the hydrogel is composed of one or a combination of a plurality of materials selected from collagen, gelatin, hyaluronic acid, Matrigel, GelMA, ColMA, fibrin, cellulose, and hemicellulose, agarose, and alginic acid.

13. A platform body having a culture solution storage space formed by introducing one side, and having a culture space portion partitioned by a partition wall within the culture space portion and having at least three sides exposed by the culture solution storage space portion, a first passage portion connected to the culture space portion partitioned by the partition wall, and a second passage portion formed on the upper side of the culture solution storage space portion, and at least one culture solution supply passage formed for supplying culture solution to the culture space portion with the upper surface side of the partition wall partitioning the culture space portion; A vertical platform for culturing and observing tissues or cells, characterized by having a transparent plate member that seals an open culture space and a culture solution storage space by being joined or adhered to a partition wall on one side of the platform body.

14. A platform having a transparent platform body, a culture space having a first passage formed on one side of the platform body, and a culture solution storage space having a second passage formed thereon, a partition wall having at least one culture solution supply passage formed therein for supplying culture solution from the culture solution storage space to the culture space, and a transparent plate member that is coupled to the platform body and seals the culture space and the culture solution storage space divided by the partition wall, On the side corresponding to the above shooting unit, a lighting unit is installed to irradiate light to the platform being shot, A vertical platform monitoring system for culturing tissues or cells, characterized by having a control server that controls the above-mentioned photographing unit, lighting unit, and measuring unit, and includes a monitor for displaying the photographed image.

15. In claim 14, A vertical platform monitoring system for culturing tissues or cells, characterized in that the above-mentioned photographing unit and the corresponding stage are installed on the back of the platform and the lighting unit further includes a fluorescent lighting source.

16. In paragraph 14 or 15, It has a first electrode embedded in the above platform body and an end exposed to the culture space, and a second electrode whose end is exposed to the culture solution storage space. A vertical platform monitoring system for culturing tissues or cells, characterized in that it can measure the electrical resistance between the culturing space and the culturing solution storage space in real time using the first and second electrodes, and further comprises the TEEL measuring unit for providing an appropriate stimulus to the culturing cells through the first and second electrodes.

17. In paragraph 16, The above control server includes a collection unit that collects information captured by a shooting unit, a diagnosis unit that diagnoses the cell culture status using the collected cell culture information, and a storage unit for storing the collection information collected by the collection unit and the information diagnosed by the diagnosis unit. A vertical platform monitoring system for culturing tissues or cells, characterized in that it controls the TEEL measurement unit to control the culture state of tissues and cells and includes a control unit for controlling a photographing unit.

Images