Animal behavior detection device and method based on infrared laser light sheet

Abstract

An animal behavior detection device and method based on an infrared laser light sheet. The detection device comprises an experimental platform module (1), an optical path module (2), an imaging module (3), and a control module (4). The experimental platform module (1) comprises a hole (131) that is through in the up-down direction and a light-transmitting hole (141); the hole (131) is used for placing an animal for experimentation; the optical path module (2) adopts a Powell prism structure; and a one-dimensional laser line generated by the optical path module (2) is aligned with the light-transmitting hole (141). When the animal behavior detection device is in use, the one-dimensional laser line generated by the optical path module (2) fills the light-transmitting hole (141); a first camera (31) of the imaging module (3) is used for recording changes in a reflected light signal, and a second camera (32) uses a beam splitter (33) to record animal behaviors; the control module (4) is connected to the first camera (31) and the second camera (32), and is used for sending trigger signals to the first camera (31) and the second camera (32), and then recording image data returned by the first camera (31) and the second camera (32) for subsequent experimental analysis. The device features a simple structure, has flexibility and adaptability, and allows parameters such as light source wavelength and spacer height to be adjusted on the basis of the characteristics of experimental animals.

Description

Animal behavior detection device and method based on infrared laser sheet TECHNICAL FIELD

[0001] The present application belongs to the technical field of animal behavior detection, and particularly relates to an animal behavior detection device and method based on an infrared laser sheet. BACKGROUND

[0002] Animal behavior research can be used in drug test, psychology research, neurobiology research and other fields, and promotes a number of major discoveries in life sciences. However, many animal behavior experiments will cause irreversible trauma to experimental animals, which will affect the experimental results to some extent and bring challenges to experimental ethics. In addition, many behavior detections need to be done in animal behavior experiments, including whether the behavior occurs, the duration of the behavior, the degree of progress, etc., and these indicators need to be quantified.

[0003] In neurobiology research, scientists often use various methods to quantify the behavior of experimental animals. However, insects are small in size and behave quickly, which brings difficulties to behavior detection.

[0004] Chinese patent CN208064278U discloses a semi-open wind tunnel device for insect behavior and chemical ecology research, which belongs to the field of insect behavior and ecology research, and includes a wind tunnel main frame in a semi-closed frame structure surrounded by a wind tunnel left side wall, a wind tunnel right side wall, a wind tunnel front wall, a wind tunnel rear wall and a wind tunnel bottom wall. An air inlet is provided on the wind tunnel front wall, and an air source is provided at the front end of the air inlet. An air outlet is provided on the wind tunnel rear wall. An insect feeding platform is mounted on the wind tunnel main frame. A cylindrical outer insect cage is mounted on the insect feeding platform. The lower end of the outer insect cage is placed in the cavity of the wind tunnel main frame. The lower end of the side wall of the outer insect cage is provided with an insect outlet. A control member is mounted on the outer insect cage to open and close the insect outlet. The device places an odor source in the wind tunnel main frame, and the wind tunnel main frame has flowing air, providing a more natural experimental space. However, the device is only a small wind tunnel and does not have optical detection function.

[0005] A new device for testing the olfactory behavior of small moths is disclosed in Chinese patent CN208925005U. The device includes a miniature air pump with its outlet connected to an activated carbon filter column via a gas guide tube. The outlet of the activated carbon filter column is connected to a gas flow meter via a gas guide tube. The outlet of the gas flow meter is connected to a water filter column via a gas guide tube. The outlet of the water filter column is connected to a three-way connector via a gas guide tube. The other two ends of the three-way connector are connected to the behavior testing device via gas guide tubes. This device can simultaneously test the behavioral responses of 20-30 test insects to the odor substances being tested, significantly improving work efficiency. The cold light source incandescent lamp ensures that the test insects in the activity chamber receive consistent light intensity in all directions, effectively avoiding the influence of light intensity differences on the movement orientation of the test insects, thereby affecting their behavioral selection in response to different odor stimuli. However, this device only tests the olfactory behavior of small moth insects, and cannot well detect changes in other behavioral details such as feeding, exploration, and gait changes. SUMMARY

[0006] Based on the current situation of the lack of devices capable of flexibly detecting animal behavior details such as feeding, exploration, and gait changes in the prior art, the present application provides an animal behavior detection device and method based on infrared laser light sheets.

[0007] The object of the present application can be achieved by the following technical solutions:

[0008] The present application provides an animal behavior detection device based on infrared laser light sheets, which includes a test bench module, a light path module, an imaging module, and a control module,

[0009] The test bench module includes a top plate, a bottom plate, a middle plate, and a cushion layer,

[0010] The top plate, middle plate, cushion layer, and bottom plate are laid in order from top to bottom. The top plate and bottom plate are both transparent materials, and the middle plate is opaque. The middle plate has holes that pass through from top to bottom. The holes are used to place animals for testing. The cushion layer has a light transmission hole. The holes and the light transmission hole are connected.

[0011] The light path module uses a Powell prism structure, which can uniformly distribute the collimated light beam and expand it into a uniform light sheet. The uniform light sheet produced by the light path module is aligned with the light transmission hole. When the animal behavior detection device is in use, the uniform light sheet produced by the light path module fills the light transmission hole.

[0012] The imaging module comprises a first camera, a second camera and a beam splitter, the first camera is arranged below and parallel to the bottom plate, the second camera is arranged below and perpendicular to the bottom plate and directly below the hole, the beam splitter is arranged below the bottom plate and directly below the hole, and the beam splitter is also arranged at the intersection of the routes of the first camera and the second camera, wherein the first camera is used for recording changes of reflected light signals, and the second camera records animal behaviors by using the beam splitter.

[0013] The control module is connected with the first camera and the second camera, is used for sending a trigger signal to the first camera and the second camera, and then recording image data returned by the first camera and the second camera for subsequent experimental analysis.

[0014] In an embodiment of the present application, the top plate uses a transparent acrylic plate, which can facilitate observation of behaviors of small animals.

[0015] In an embodiment of the present application, the bottom plate uses transparent inorganic glass, which can prevent scratching.

[0016] In an embodiment of the present application, the middle plate uses a milky white acrylic plate, which can shield the laser light source.

[0017] In an embodiment of the present application, the thickness of the middle plate satisfies that the animal to be detected can move in the hole.

[0018] In an embodiment of the present application, the animal is a small insect, including but not limited to a fruit fly.

[0019] In an embodiment of the present application, four pads are arranged, and the pads are arranged at four corners of the bottom plate, the pad is made of frosted glass paper, and the thickness of the pad is 0.1 mm, so that a light transmission hole with a thickness of 0.1 mm is formed.

[0020] In an embodiment of the present application, the light path module adopts a Powell prism structure, and comprises an infrared light source, a laser, a three-axis steering micro-operation platform, a two-axis steering micro-operation platform, a Powell prism and a steering micro-operation platform.

[0021] The laser is mounted on the three-axis steering micro-operation platform, the steering micro-operation platform is arranged in front of the laser, the steering micro-operation platform is mounted on the two-axis steering micro-operation platform, the Powell prism is mounted on the steering micro-operation platform, the Powell prism is used for uniformly distributing the Gaussian distribution collimated light beam generated by the laser and expanding the light beam into a uniform light sheet, and the infrared light source is arranged above the test bench module and is used for providing a light source for the imaging module.

[0022] The infrared light source is used for providing illumination light for camera imaging of the imaging module.

[0023] The three-axis steering micro-operation platform is used for adjusting the position and angle of the laser, and the two-axis steering micro-operation platform is used for adjusting the position and angle of the steering micro-operation platform.

[0024] The three-axis steering micro-operation platform, the two-axis steering micro-operation platform and the steering micro-operation platform are used in combination to adjust the angle of the light source and the Powell prism as a whole, finally form a light sheet and improve the uniformity of the light sheet.

[0025] The three-axis steering micro-operation platform is mainly capable of realizing movement and adjustment in three vertical directions, the two-axis steering micro-operation platform is mainly capable of realizing movement and adjustment in two vertical directions, and the steering micro-operation platform is used for mounting the Powell prism and realizing fine adjustment of the position or angle of the Powell prism.

[0026] The three-axis steering micro-operation platform is composed of a first axial displacement table, a right-angle fixed block, a second axial displacement table, a third axial displacement table and a first rotary displacement table, wherein the third axial displacement table is arranged at the lowermost layer, the second axial displacement table is arranged above the third axial displacement table, the first axial displacement table is fixed on the second axial displacement table through the right-angle fixed block, the first rotary displacement table is arranged on the first axial displacement table, and the laser is arranged on the first rotary displacement table, wherein the first axial displacement table is used for realizing movement in the vertical direction, the second axial displacement table and the third axial displacement table are used for realizing movement in the horizontal direction, the movement directions of the second axial displacement table and the third axial displacement table are perpendicular to each other, and the second rotary displacement table is used for realizing rotation.

[0027] The two-axis steering micro-operation platform is composed of a fourth axial displacement table, a fifth axial displacement table and a second rotary displacement table, the fourth axial displacement table is arranged above the fifth axial displacement table, the fourth axial displacement table and the fifth axial displacement table are used for realizing movement in the horizontal direction, the movement directions of the fourth axial displacement table and the fifth axial displacement table are perpendicular to each other, the second rotary displacement table is arranged on the fourth axial displacement table, the second rotary displacement table is used for realizing rotation, and the steering micro-operation platform is mounted on the second rotary displacement table.

[0028] The steering micro-operation platform comprises a first support and a second support, the first support is connected to the two-axis steering micro-operation platform through a connecting rod, the Powell prism is fixed on the second support, and the second support is connected to the first support,

[0029] The first support is provided with a threaded hole, the second support is provided with a fine adjustment bolt matched with the threaded hole, the connection of the second support with the first support is realized through the connection of the fine adjustment bolt with the threaded hole, and the middle of the first support is a hole for accommodating the Powell prism.

[0030] In an embodiment of the present application, the connecting rod can be a telescopic rod with adjustable length.

[0031] In an embodiment of the present application, four threaded holes and four fine adjustment bolts are respectively arranged and uniformly distributed around the circumference of the hole. Since the placing angle of the Powell prism is directly related to the angle and uniformity of the light sheet, the second support for fixing the Powell prism is further provided with a fine adjustment bolt, when the second support is connected with the first support, the placing angle of the Powell prism can be finely adjusted by adjusting the tightness of the cooperation of the fine adjustment bolt with the threaded hole, and the two-axis steering micro-operation platform is used for adjusting the front and back and left and right positions of the Powell prism, and in use, the two-axis steering micro-operation platform is first adjusted, and then the fine adjustment bolt is adjusted.

[0032] In an embodiment of the present application, the first, second, third, fourth and fifth axial displacement tables are all manual axial displacement tables, for example, a precision translation table with a model number of PTS100M of BOCIC can be selected. The first and second rotary displacement tables are all manual rotary displacement tables, for example, an inch manual rotary displacement table with a model number of MSRP01 of Sorebo can be selected. In an embodiment of the present application, the right-angle fixing block can be a right-angle fixing block with a model number of RAB102 of BOCIC.

[0033] The light path module is arranged in the present application to obtain a uniform light sheet, specifically, the Powell prism is used to re-distribute the light power of the collimated light beam with Gaussian distribution and expand it into a uniform light sheet. Compared with a cylindrical lens, the Powell prism can eliminate the central hot spot and fading edge distribution of the Gaussian light beam to realize better line uniformity. Within a range of 80% of the center symmetry of the laser line, the non-uniformity is <30%. In the light path module, multiple micro-operation platforms are designed to make the distance and angle flexible and adjustable.

[0034] In an embodiment of the present application, the Gaussian distribution collimated light beam used by the light path module selects far infrared light with a wavelength of 1066nm. For most experimental animals, far infrared light is invisible light source and will not affect the vision of small animals during the experiment, therefore, the Gaussian distribution collimated light beam used by the light path module selects far infrared light with a wavelength of 1066nm.

[0035] In an embodiment of the present application, the first camera and the second camera are both industrial cameras.

[0036] In an embodiment of the present application, the first camera (31) is a deep infrared camera, and the second camera (32) is an infrared camera.

[0037] In an embodiment of the present application, the control module comprises a host computer, a display, a keyboard, a mouse and a junction box, the host computer is connected with the display, the keyboard, the mouse and the junction box respectively, the junction box is further connected with the first camera and the second camera, the junction box is used to send a trigger signal to the first camera and the second camera, and the image data returned by the two cameras is transmitted to the host computer for subsequent experimental analysis.

[0038] The device of the present application, after being matched with the optical path module and the experimental bench module, uniformly distributes the infrared light sheet through the light transmission hole of the experimental bench, so that the space of 0.1 mm from the bottom plate is filled with far infrared light when the small animal experiment is performed, and when any part of the body of the small animal enters the far infrared light area, strong reflection will be formed. The reflection signal and the real-time behavior of the small animal are recorded by the imaging module. One camera records the change of the reflection signal, and the other camera records the behavior of the small animal by using a beam splitter.

[0039] The present application also provides an animal behavior detection method based on an infrared laser light sheet, which is based on the animal behavior detection device and comprises the following steps:

[0040] S1, placing a bottom plate, a cushion layer, a middle plate, placing a to-be-detected animal, placing a top plate, and assembling an experimental bench module;

[0041] S2, adjusting the position of the optical path module, and turning on a laser light source;

[0042] S3, turning on an imaging module and a control module, and triggering the imaging module to shoot the behavior of the to-be-detected animal;

[0043] S4, the control module analyzes the behavior data of the to-be-detected animal.

[0044] Compared with the prior art, the present application has the following advantages and beneficial effects:

[0045] 1. High experimental accuracy. Conventional video shooting cannot determine the behavior details of a small animal in free activity, but the present application can accurately determine whether the behavior details (such as eating, exploration and gait change) occur with the aid of infrared light assisted detection.

[0046] 2. Safe and non-invasive. The infrared light detection method does not require implanting sensors or other additional operations on the animal, and the impact on the experimental animal is minimized.

[0047] 3、The device of the present application has simple structure and is flexible and variable. The wavelength of light source and the height of cushion layer can be changed according to the characteristics of experimental animals. BRIEF DESCRIPTION OF DRAWINGS

[0048] Fig. 1 is a structural schematic diagram of the animal behavior detection device based on infrared laser light sheet in embodiment 1 of the present application;

[0049] Fig. 2 is an exploded structural schematic diagram of the test bench module in embodiment 1;

[0050] Fig. 3 is a combined structural schematic diagram of the test bench module in embodiment 1;

[0051] Fig. 4 is a structural schematic diagram of the light path module in embodiment 1;

[0052] Fig. 5 is a detailed structural schematic diagram of the light path module in embodiment 1;

[0053] Fig. 6 is a structural schematic diagram of the imaging module in embodiment 1;

[0054] Fig. 7 is a structural schematic diagram of the control module in embodiment 1;

[0055] Fig. 8 is a work flow diagram of the animal behavior detection method based on infrared laser light sheet;

[0056] Fig. 9 is a screenshot of experimental image of fruit fly experiment in embodiment 2;

[0057] Fig. 10 is the experimental result of fruit fly experiment in embodiment 2. DETAILED DESCRIPTION

[0058] The present application will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0059] Embodiment 1

[0060] Referring to Fig. 1 and combining Figs. 2-7, the present embodiment provides an animal behavior detection device based on infrared laser light sheet, which comprises a test bench module 1, a light path module 2, an imaging module 3 and a control module 4.

[0061] Further referring to Figs. 2 and 3, the test bench module 1 comprises a top plate 11, a bottom plate 12, a middle plate 13 and a cushion layer 14, which are laid in order from top to bottom, the top plate 11 and the bottom plate 12 are both transparent materials, the middle plate 13 is opaque, the middle plate 13 is provided with a hole 131 passing through from top to bottom, the hole 131 is used for placing animals to be experimented, the cushion layer 14 is formed with a light transmission hole 141, and the hole 131 and the light transmission hole 141 are in communication;

[0062] The light path module 2 adopts a Powell prism structure, can uniformly distribute the Gaussian distribution collimated light beam 27, and expand it into a layer of uniform light sheet 28. The light path module 2 generates a layer of uniform light sheet 28 aligned with the light transmission hole 141. When the animal behavior detection device is used, the light path module 2 generates a layer of uniform light sheet 28 to fill the light transmission hole 141.

[0063] Further referring to FIG. 6, the imaging module 3 includes a first camera 31, a second camera 32, and a beam splitter 33. The first camera 31 is arranged below and parallel to the bottom plate 12. The second camera 32 is arranged below and perpendicular to the bottom plate 12 and directly below the hole 131. The beam splitter 33 is arranged below the bottom plate 12 and directly below the hole 131. The beam splitter 33 is also arranged at the intersection of the routes of the first camera 31 and the second camera 32. The first camera 31 is used to record the change of the reflected light signal, and the second camera 32 records the animal behavior by using the beam splitter 33.

[0064] The control module 4 is connected with the first camera 31 and the second camera 32, and is used to send a trigger signal to the first camera 31 and the second camera 32. Then, the image data returned by the first camera 31 and the second camera 32 is recorded for subsequent experimental analysis.

[0065] In this embodiment, the top plate 11 uses a transparent acrylic plate, which can facilitate observation of the behavior of small animals. The bottom plate 12 uses transparent inorganic glass, which can prevent scratching. The middle plate 13 uses a milky white acrylic plate, which can block the laser light source. The thickness of the middle plate 13 satisfies the movement of the animal to be detected in the hole 131. The animal is a small insect, including but not limited to fruit flies. The four cushion layers 14 are arranged at the four corners of the bottom plate 12, respectively. The cushion layer 14 adopts frosted glass paper with a thickness of 0.1 mm, thereby forming a light transmission hole 141 with a thickness of 0.1 mm.

[0066] In this embodiment, the light path module 2 adopts a Powell prism structure, including an infrared light source 21, a laser 22, a three-axis steering micro-operation platform 23, a two-axis steering micro-operation platform 24, a Powell prism 25, and a steering micro-operation platform 26.

[0067] Further referring to FIG. 4, the laser 22 is installed on the three-axis steering micro-operation platform 23. The steering micro-operation platform 26 is arranged in front of the laser 22. The steering micro-operation platform 26 is installed on the two-axis steering micro-operation platform 24. The Powell prism 25 is installed on the steering micro-operation platform 26. The Powell prism 25 is used to uniformly distribute the Gaussian distribution collimated light beam 27 generated by the laser 22 and expand it into a layer of uniform light sheet 28. The infrared light source 21 is arranged above the test bench module 1 and is used to provide a light source for the imaging module 3.

[0068] In the embodiment, the infrared light source 21 is used to provide illumination light for camera imaging of the imaging module 3. The setting position of the infrared light source 21 has no relationship with the laser 22, and the setting position of the infrared light source 21 only needs to meet the light source required by camera imaging of the imaging module 3.

[0069] In the optical path module of the embodiment, the three-axis steering micro-operation platform 23 is used to adjust the position and angle of the laser 22, and the two-axis steering micro-operation platform 24 is used to adjust the position and angle of the steering micro-operation platform 26. In the optical path module of the embodiment, the three-axis steering micro-operation platform 23, the two-axis steering micro-operation platform 24 and the steering micro-operation platform 26 are used in combination to adjust the angle of the light source and the Powell prism as a whole, so as to finally form a light sheet and improve the uniformity of the light sheet.

[0070] Further referring to FIG. 5, in the optical path module of the embodiment, the three-axis steering micro-operation platform 23 is mainly capable of realizing movement and adjustment in three vertical directions, the two-axis steering micro-operation platform 24 is mainly capable of realizing movement and adjustment in two vertical directions, and the steering micro-operation platform 26 is used to install the Powell prism 25 and realize fine adjustment of the position or angle of the Powell prism 25.

[0071] Further referring to FIG. 5, the three-axis steering micro-operation platform 23 is composed of a first axial displacement table 231, a right-angle fixed block 232, a second axial displacement table 233, a third axial displacement table 234 and a first rotary displacement table 235, wherein the third axial displacement table 234 is arranged at the lowermost layer, the second axial displacement table 233 is arranged above the third axial displacement table, the first axial displacement table 231 is fixed on the second axial displacement table 233 through the right-angle fixed block 232, the first rotary displacement table 235 is arranged on the first axial displacement table 231, and the laser 22 is arranged on the first rotary displacement table 231. The first axial displacement table is used to realize movement in the vertical direction, the second axial displacement table and the third axial displacement table are used to realize movement in the horizontal direction, and the movement directions of the second axial displacement table and the third axial displacement table are perpendicular to each other, and the second rotary displacement table is used to realize rotation.

[0072] Further referring to FIG. 5, the two-axis steering micro-operation platform 24 is composed of a fourth axial displacement table 241, a fifth axial displacement table 242, and a second rotary displacement table 243. The fourth axial displacement table 241 is arranged above the fifth axial displacement table 242, and the fourth axial displacement table and the fifth axial displacement table are used to realize horizontal movement, and the movement directions of the fourth axial displacement table and the fifth axial displacement table are perpendicular to each other. The second rotary displacement table 243 is arranged on the fourth axial displacement table 241, and the second rotary displacement table 243 is used to realize rotation. The steering micro-operation platform 26 is installed on the second rotary displacement table 243.

[0073] Further referring to FIG. 5, the steering micro-operation platform 26 includes a first support 261 and a second support 262. The first support 261 is connected to the two-axis steering micro-operation platform 24 through a connecting rod 263. The Powell prism 25 is fixed on the second support 262, and the second support 262 is connected to the first support 261.

[0074] The first support 261 is provided with a threaded hole 264, and the second support 262 is provided with a fine adjustment bolt 265 matched with the threaded hole 264. The connection between the second support 262 and the first support 261 is realized through the connection between the fine adjustment bolt 265 and the threaded hole 264. The middle of the first support 261 is a hole 266 for accommodating the Powell prism 25.

[0075] The connecting rod 263 can be selected as a telescopic rod with adjustable length.

[0076] Further referring to FIG. 5, in the embodiment, four threaded holes 264 and fine adjustment bolts 265 are arranged respectively and uniformly distributed around the circumference of the hole 266. Since the placement angle of the Powell prism 25 is directly related to the angle and uniformity of the light sheet, the second support 262 for fixing the Powell prism 25 is further provided with fine adjustment bolts 265. When the second support 262 is connected to the first support 261, the placement angle of the Powell prism 25 can be fine adjusted by adjusting the tightness of the cooperation between the fine adjustment bolt 265 and the threaded hole 264. The two-axis steering micro-operation platform 24 is used to adjust the front, back, left and right positions of the Powell prism 25. In use, the two-axis steering micro-operation platform 24 is adjusted first, and then the fine adjustment bolt 265 is adjusted.

[0077] More specifically, in the embodiment, the first axial displacement table, the second axial displacement table, the third axial displacement table, the fourth axial displacement table, and the fifth axial displacement table are all manual axial displacement tables, which can be selected as the precision translation table with model PTS100M of BOCIC. The first rotary displacement table and the second rotary displacement table are both manual rotary displacement tables, which can be selected as the MSRP01 rotary displacement table of Sorebo. An inch manual rotary displacement table. The right-angle fixed block can be selected from the right-angle fixed block of model RAB102 of BOCIC.

[0078] In this embodiment, the light path module is arranged to obtain a uniform light sheet. Specifically, the Powell prism is used to re-distribute the light power of the collimated light beam with Gaussian distribution and expand it into a uniform light sheet. Compared with the cylindrical lens, the Powell prism can eliminate the central hot spot and fading edge distribution of the Gaussian light beam to achieve better line uniformity. Within the 80% range of the center symmetry of the laser line, the non-uniformity is less than 30%. In the light path module, multiple micro-operation platforms are designed to make the distance, angle and other parameters flexible and adjustable.

[0079] In this embodiment, the Gaussian distribution collimated light beam used by the light path module 2 selects far infrared light with a wavelength of 1066nm. For most experimental animals, far infrared light is invisible light source and will not affect the vision of small animals during the experiment, so the Gaussian distribution collimated light beam used by the light path module selects far infrared light with a wavelength of 1066nm.

[0080] In this embodiment, the first camera 31 and the second camera 32 are both industrial cameras. More specifically, the first camera 31 is a deep infrared camera, and the second camera 32 is an infrared camera.

[0081] Further referring to FIG. 7, in this embodiment, the control module 4 includes a host computer 41, a display 42, a keyboard 43, a mouse 44 and a junction box 45, the host computer 41 is connected with the display 42, the keyboard 43, the mouse 44 and the junction box 45 respectively, and the junction box 45 is further connected with the first camera 31 and the second camera 32. The junction box sends a trigger signal to the first camera 31 and the second camera 32, and transmits the image data returned by the two cameras to the host computer 41 for subsequent experimental analysis.

[0082] The device provided in this embodiment is used after the light path module and the experimental table module are matched. The uniform light sheet passes through the light transmission hole of the experimental table, so that the space of 0.1mm from the bottom plate is filled with far infrared light when the small animal is experimented. When any part of the body of the small animal enters the far infrared light area, strong reflection will be formed. The reflection signal and the real-time behavior of the small animal will be recorded by the imaging module. One camera records the change of the reflection signal, and the other camera records the behavior of the small animal by using a beam splitter.

[0083] This embodiment also provides an animal behavior detection method based on an infrared laser light sheet, which is based on the animal behavior detection device and has a work flow chart as shown in FIG. 8, and includes the following steps:

[0084] S1, place the bottom plate 12, the cushion layer 14 and the middle plate 13, place the top plate 11, assemble the experimental table module;

[0085] S2, adjust the position of the light path module 2, turn on the laser light source;

[0086] S3, turn on the imaging module 3 and the control module 4, trigger the imaging module 3 to shoot the behavior of the animal to be measured;

[0087] S4, the control module 4 analyzes the ethological data of the animal to be measured.

[0088] Embodiment 2

[0089] Based on the specific infrared laser light sheet-based animal ethology detection device and the infrared laser light sheet-based animal ethology detection method provided in Embodiment 1, taking the walking behavior of fruit flies as an example, fruit flies need to alternately lift and land 6 legs, when the leg is lifted off the ground, it is out of the far infrared light area and does not form a reflection, and when the leg contacts the ground, it forms a reflection, which can be used for gait analysis of fruit flies.

[0090] Taking gait analysis as an example, a certain specific descending neuron X of fruit flies is subjected to light inhibition test (i.e. when 505nm green light is irradiated, the specific descending neuron will be inhibited), and image data of the fruit fly in normal state and inhibited state are collected, which are compared with the normal fruit fly, and the experimental image screenshots are shown in FIG. 9. FIG. 9 (left) is an example of an image taken by the infrared camera 32, and FIG. 9 (middle and right) are examples of images taken by the deep infrared camera 31, in which the red circles are the reflection signals of the six legs of the small animal (fruit fly) on the light sheet. According to the image data, it can be judged whether the six legs of the fruit fly are on the ground at each time, the landing position and other information, and further gait data (standing time of the inner back leg when turning, step frequency, step length, etc.) are obtained, and the results are shown in FIG. 10. The upper graph of FIG. 10 is a schematic diagram of the small animal stepping on the light sheet; and the lower graph of FIG. 10 is a violin plot of three gait analysis indicators (standing time of the inner back leg when turning, step frequency, step length) of the small animal under different experimental conditions. Taking the standing time of the inner back leg when turning as an example, the control group is a normal fruit fly, and the X group is a light-inhibited transgenic fruit fly, the control group includes 365 groups of ON data (when the light is on) and 390 groups of OFF data (when the light is off), and the X group includes 357 groups of ON data (when the light is on, i.e. when the specific neuron is inhibited) and 471 groups of OFF data (when the light is off, i.e. when the neuron is not inhibited), it can be found that after the specific neuron X is inhibited, the standing time of the inner back leg when the fruit fly turns is shortened.

[0091] Similarly, the device can be used to analyze feeding behavior and social behavior.

[0092] The foregoing description of the embodiments has been presented for the purpose of illustration and description. It is not intended to be exhaustive or to limit the application to the precise form disclosed. Modifications and variations are possible in light of the above teachings or can be acquired from practice of the application. As well, the description is presented in the context of the preferred embodiments as a number of alternatives. It is not intended to limit the application to the precise form described.

Claims

1. An animal behavior detection device based on an infrared laser sheet, characterized in that, It comprises a test bench module (1), a light path module (2), an imaging module (3) and a control module (4), The test bench module (1) comprises a top plate (11), a bottom plate (12), a middle plate (13) and a cushion layer (14), The top plate (11), the middle plate (13), the cushion layer (14) and the bottom plate (12) are sequentially laid from top to bottom, the top plate (11) and the bottom plate (12) are both transparent materials, the middle plate (13) is opaque, the middle plate (13) is provided with a hole (131) penetrating from top to bottom, the hole (131) is used for placing an animal to be tested, and the cushion layer (14) is formed with a light transmission hole (141), and the hole (131) and the light transmission hole (141) are in communication; The light path module (2) adopts a Powell prism structure, can uniformly distribute a Gaussian distribution collimated light beam (27) again, and expand it into a layer of uniform light sheet (28); when the animal behavior detection device is used, the light sheet (28) generated by the light path module (2) fills the light transmission hole (141); The imaging module (3) comprises a first camera (31), a second camera (32) and a beam splitter (33), the first camera (31) is arranged below the bottom plate (12) and parallel to the bottom plate (12), the second camera (32) is arranged below the bottom plate (12) and perpendicular to the bottom plate (12) and directly below the hole (131), the beam splitter (33) is arranged below the bottom plate (12) and directly below the hole (131), and the beam splitter (33) is also arranged at the intersection of the routes of the first camera (31) and the second camera (32), wherein the first camera (31) is used for recording changes of reflected light signals, and the second camera (32) records animal behaviors by using the beam splitter (33); The control module (4) is connected with the first camera (31) and the second camera (32), is used for sending a trigger signal to the first camera (31) and the second camera (32), then recording image data returned by the first camera (31) and the second camera (32) for subsequent experimental analysis.

2. The animal behavior detection device based on infrared laser sheet according to claim 1, wherein, The top plate (11) uses a transparent acrylic plate, the bottom plate (12) uses transparent inorganic glass, and the middle plate (13) uses a milky white acrylic plate to shield the laser light source.

3. The animal behavior detection device based on infrared laser sheet according to claim 1, wherein, The thickness of the middle plate (13) satisfies that the animal to be detected can move in the hole (131), and the animal is a small insect.

4. The animal behavior detection device based on infrared laser sheet according to claim 1, wherein, The four cushion layers (14) are arranged at four corners of the bottom plate (12), the cushion layer (14) adopts frosted glass paper with a thickness of 0.1 mm, and the light transmission hole (141) with a thickness of 0.1 mm is formed.

5. The animal behavior detection device based on infrared laser sheet according to claim 1, wherein, The light path module (2) adopts a Powell prism structure and comprises an infrared light source (21), a laser (22), a three-axis steering micro-operation platform (23), a two-axis steering micro-operation platform (24), a Powell prism (25) and a steering micro-operation platform (26), The laser (22) is installed on a three-axis steering micro-operation platform (23), a steering micro-operation platform (26) is arranged in front of the laser (22), the steering micro-operation platform (26) is installed on a two-axis steering micro-operation platform (24), the Powell prism (25) is installed on the steering micro-operation platform (26), and the Powell prism (25) is used for uniformly distributing the Gaussian distribution collimated light beam (27) generated by the laser (22) and expanding the Gaussian distribution collimated light beam (27) into a uniform light sheet (28); and the infrared light source (21) is arranged above the test bench module (1) and is used for providing a light source for the imaging module (3).

6. The animal ethology detection device based on infrared laser sheet according to claim 5, characterized in that, The steering micro-operation platform (26) comprises a first support (261) and a second support (262), the first support (261) is connected to the two-axis steering micro-operation platform (24) through a connecting rod (263), the Powell prism (25) is fixed on the second support (262), the second support (262) is connected to the first support (261), screw holes (264) are arranged on the first support (261), the second support (262) is provided with fine adjustment bolts (265) matched with the screw holes (264), and the connection between the second support (262) and the first support (261) is realized through the connection of the fine adjustment bolts (265) and the screw holes (264).

7. The animal behavior detection device based on infrared laser sheet according to claim 1, wherein, The Gaussian distribution collimated light beam of the laser (22) is far infrared light with a wavelength of 1066 nm.

8. The animal behavior detection device based on infrared laser sheet according to claim 1, wherein, The control module (4) comprises a host computer (41), a display (42), a keyboard (43), a mouse (44) and a junction box (45), the host computer (41) is connected with the display (42), the keyboard (43), the mouse (44) and the junction box (45) respectively, the junction box (45) is also connected with the first camera (31) and the second camera (32), a trigger signal is sent to the first camera (31) and the second camera (32) by using the junction box, image data returned by the two cameras is transmitted to the host computer (41) and is used for subsequent experimental analysis.

9. An infrared laser sheet based ethology detection method, characterized by, The animal behavior detection device based on any one of claims 1-8 is performed, comprising the following steps: S1, placing the bottom plate (12), the cushion layer (14) and the middle plate (13), placing the top plate (11), assembling the test bench module; S2, adjusting the position of the light path module (2) and turning on the laser light source; S3, turning on the imaging module (3) and the control module (4), triggering the imaging module (3) to shoot the behavior of the animal to be detected; S4, the control module (4) analyzes the behavior data of the animal to be detected.

10. The method of claim 9, wherein the infrared laser sheet is a 1064 nm laser sheet. After the light path module and the test bench module are matched, the uniform light sheet passes through the light transmission hole of the test bench, so that the space of 0.1 mm from the bottom plate (12) is filled with far infrared light when the small animal experiment is performed, a strong reflection is formed when any part of the body of the small animal enters the far infrared light area, and the reflection signal and the real-time behavior of the small animal are recorded by the imaging module, one camera records the change of the reflection signal, and the other camera records the behavior of the small animal by using a beam splitter.

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