Anti-wrinkle composition, and preparation method therefor and use thereof

Abstract

An anti-wrinkle composition, and a preparation method therefor and a use thereof. The anti-wrinkle composition comprises the following components in parts by weight: 0.1-1.5 parts of aphanothece sacrum polysaccharide, 0.1-70 parts of gloiopeltis furcata extract, 10-230 parts of butanediol, and 50-600 parts of water. By means of the synergistic effect of the aphanothece sacrum polysaccharide and the gloiopeltis furcata extract, the hydrating, moisturizing, and skin elasticity improvement and anti-wrinkle effects of the anti-wrinkle composition are improved.

Description

Anti-wrinkle composition and preparation method and application thereof TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of cosmetics, in particular to an anti-wrinkle composition and preparation method and application thereof. BACKGROUND

[0002] With the growth of age, the metabolism of human body begins to slow down, the water and subcutaneous fat decrease, the elastic fiber gradually thickens, the skin wrinkles and the luster fades. Skin aging is a prominent manifestation of the aging of the whole body, among which the aging of facial skin is particularly obvious. The number of fibroblasts in the human body gradually decreases, and the collagen and elastin secreted by the fibroblasts also gradually decrease and break due to oxidation, so the support to the epidermis disappears, causing uneven collapse. The dermis layer begins to thin, the skin elasticity becomes poor, and wrinkles begin to gradually appear. In addition to natural physiological factors, the aging of human skin is also related to long-term sleep quality, ultraviolet radiation, working environment, living habits, nutritional status and skin care, etc.

[0003] Although skin aging is an irreversible physiological phenomenon, the aging process can be slowed down through medical aesthetics and the use of cosmetics. The existing wrinkle removal methods on the market are as follows: (1) a small amount of botulinum toxin type A is injected, but this method may cause serious complications, which poses a serious risk to the health of the skin; (2) peptides and peptide compositions are used to achieve the effect of tightening and anti-wrinkles, but the active ingredients cannot be well absorbed by the skin and only stay on the surface, so the anti-wrinkle effect is poor; (3) dimethicone is used to fill the pores and gaps in the skin to achieve an instant wrinkle removal effect, but this method only treats the symptoms and does not treat the root cause. Long-term use of this method can block the pores and cause irritation to the skin, leading to acne or allergic reactions; (4) retinol products are used to resist wrinkles, which have a relatively outstanding effect, but high-concentration retinol can irritate the skin and its stability is poor, which limits its bioavailability. SUMMARY

[0004] The purpose of the present disclosure is to overcome the shortcomings of the prior art and provide an anti-wrinkle composition and preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present disclosure is as follows: an anti-wrinkle composition comprising the following components by weight: 0.1-1.5 parts of water moss seaweed polysaccharide, 0.1-70 parts of sea moss extract, 10-230 parts of butanediol, and 50-600 parts of water.

[0006] In one embodiment, the mass of the water moss seaweed polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition.

[0007] In one embodiment, the mass ratio of the water-temple nori polysaccharide and the Gigartina tenella extract is 1:(1-9).

[0008] In one embodiment, the weight average molecular weight of the water-temple nori polysaccharide is (1.2-2.0) x 10 7 g / mol; and / or, the weight average molecular weight of the Gigartina tenella extract is (4.5-5.5) x 10 4 g / mol.

[0009] In one embodiment, the mass percentage of the butanediol is less than 77% based on 100% of the mass percentage of the anti-wrinkle composition.

[0010] In one embodiment, the total sugar content of the anti-wrinkle composition is 0.5-0.7%.

[0011] In another aspect, the present disclosure provides use of the anti-wrinkle composition in the preparation of a cosmetic product.

[0012] In still another aspect, the present disclosure provides a cosmetic product comprising the anti-wrinkle composition.

[0013] In one embodiment, the weight percentage of the anti-wrinkle composition in the cosmetic product is 0.5-10%.

[0014] In one embodiment, the cosmetic product further comprises at least one cosmetically acceptable auxiliary agent.

[0015] Compared with the prior art, the present disclosure has the following beneficial effects: the present disclosure uses water-temple nori polysaccharide and Gigartina tenella extract as active ingredients, the two active ingredients are combined with each other to produce sugar unit structures of N-acetyl galactosamine (GalNac) and galactose (α / βGal), and the produced sugar unit structures improve the moisturizing, water supplementing, UV damage prevention, and anti-wrinkle effects of the anti-wrinkle composition. BRIEF DESCRIPTION OF DRAWINGS

[0016] FIG. 1 is a state diagram of the anti-wrinkle composition of Comparative Example 2 after being placed under different conditions for different time periods;

[0017] FIG. 2 is a diagram of the inhibitory effect of the anti-wrinkle compositions of Comparative Examples 3-5 on different agglutinins;

[0018] FIG. 3 is a diagram of the inhibitory effect of the anti-wrinkle compositions of Comparative Examples 6-8 on different agglutinins;

[0019] FIG. 4 is a diagram of the inhibitory effect of the anti-wrinkle compositions of Examples 15-17 on different agglutinins;

[0020] FIG. 5 is a result of immunoblotting of HS68 cells induced by UVB at different concentrations of the anti-wrinkle composition;

[0021] FIG. 6 is a graph of the change in wrinkle depression depth after one of the volunteers used the eye cream of Application Example 2;

[0022] FIG. 7 is a graph of the change in crows feet after one of the volunteers used the eye cream of Application Example 2;

[0023] FIG. 8 is a graph of the change in crows feet before and after one of the volunteers used the face cream of Application Example 3;

[0024] FIG. 9 is a graph of the change in crows feet before and after one of the volunteers used the face cream of Application Example 3;

[0025] FIG. 10 is a graph of the change in under-eye wrinkles before and after one of the volunteers used the face cream of Application Example 3;

[0026] FIG. 11 is a graph of the change in under-eye wrinkles before and after one of the volunteers used the face cream of Application Example 3. DETAILED DESCRIPTION

[0027] The advantages and features of the present disclosure and a method for achieving the same will be more readily understood through reference to the following detailed description of embodiments. The present disclosure may, however, be embodied in various other forms without being limited to the embodiments set forth herein. Embodiments disclosed herein are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the present disclosure to those skilled in the art.

[0028] The terms used in the specification are merely intended to describe certain embodiments and are in no way intended to limit the present disclosure. Unless explicitly defined otherwise, expressions of the singular include the plural.

[0029] Unless otherwise defined, all terms used in the present disclosure, including technical terms and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present disclosure belongs. Any terms defined in a general dictionary should be interpreted to have the same meaning as in the context of the relevant art, and, unless explicitly defined otherwise, should not be interpreted to have idealistic or overly formalistic meanings.

[0030] Hereinafter, an anti-wrinkle composition according to the present disclosure and a cosmetic for skin moisturizing, skin water, anti-wrinkle, and prevention of ultraviolet damage containing the anti-wrinkle composition will be described in detail.

[0031] The term "skin wrinkle" used herein refers to fine lines formed on the skin due to skin weakening, which can be caused by genetic factors, reduction of collagen and elastin present in the dermis of the skin, external environment, etc.

[0032] The term "anti-skin wrinkle / anti-wrinkle" used herein refers to retarding or inhibiting the generation of wrinkles on the skin or alleviating wrinkles that have already been generated.

[0033] As used herein, the term "ultraviolet damage" refers to the skin because of the ultraviolet radiation, resulting in a large number of dermal cell necrosis and metabolic disorders, intracellular impurities can not be metabolized, resulting in skin sunburn, loss of elasticity, premature aging, become sensitive, wrinkles and other symptoms.

[0034] As used herein, the term "preventing ultraviolet damage" refers to the skin because of the ultraviolet radiation, resulting in the production of various skin symptoms.

[0035] The term "safe and effective amount" as used herein means an amount of a compound or composition sufficient to induce a positive benefit as described herein, but low enough to avoid any significant side effects judged by a skilled person.

[0036] The present disclosure provides an anti-wrinkle composition comprising the following components by weight: 0.1-1.5 parts of APHANOTHECE sacrum polysaccharide, 0.2-70 parts of Gloiopeletis furcata extract, 10-230 parts of butanediol, and 50-600 parts of water.

[0037] The anti-wrinkle composition of the present disclosure uses APHANOTHECE sacrum polysaccharide and Gloiopeletis furcata extract as active ingredients. After the combination of APHANOTHECE sacrum polysaccharide and Gloiopeletis furcata extract, the anti-wrinkle composition contains sugar unit structures such as mannose, glucose, galactose, N-acetylgalactosamine, fucose, rhamnose, and xylitol. In the first aspect, N-acetylgalactosamine and galactose can attract and retain moisture in the skin, keeping the skin moist and soft, and improving the moisturizing and skin barrier effects of the anti-wrinkle composition. In addition, N-acetylgalactosamine and galactose can also improve the soothing and anti-inflammatory effects of the anti-wrinkle composition. In the second aspect, N-acetylgalactosamine can promote the exfoliation of dead skin cells and the removal of keratin, making the skin smooth and delicate, and improving the anti-wrinkle effect of the anti-wrinkle composition. In the third aspect, the anti-wrinkle composition can synergistically enhance the Langerin lectin of Langerhans cells, regulate immune function, inhibit the production of reactive oxygen species (ROS) and matrix metalloproteinase (MMP1), enhance cell viability and proliferation, promote the expression of COL1A1 and COL1A2, and promote the production of hyaluronic acid synthase (HAS1), further improving the anti-wrinkle effect of the anti-wrinkle composition.

[0038] Exemplarily, the weight of APHANOTHECE sacrum polysaccharide can be 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1.0 parts, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, but is not limited to the listed values, other values within the scope of the present disclosure are also applicable.

[0039] Exemplarily, the weight part of the sea tangle extract can be 0.2 parts, 1 part, 5 parts, 10 parts, 13 parts, 17 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 33 parts, 35 parts, 37 parts, 40 parts, 42 parts, 45 parts, 47 parts, 50 parts, 53 parts, 55 parts, 58 parts, 60 parts, 62 parts, 65 parts, 67 parts, 70 parts, but is not limited to the listed values, and other values within the scope of the present document are also applicable.

[0040] Exemplarily, the weight part of the water can be 50 parts, 70 parts, 90 parts, 110 parts, 130 parts, 150 parts, 170 parts, 190 parts, 200 parts, 240 parts, 280 parts, 300 parts, 330 parts, 360 parts, 400 parts, 450 parts, 500 parts, 550 parts, 600 parts, but is not limited to the listed values, and other values within the scope of the present document are also applicable.

[0041] Exemplarily, the weight part of the butanediol can be 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, 100 parts, 110 parts, 120 parts, 130 parts, 140 parts, 150 parts, 160 parts, 170 parts, 180 parts, 190 parts, 200 parts, 210 parts, 220 parts, 230 parts, but is not limited to the listed values, and other values within the scope of the present document are also applicable.

[0042] In one embodiment, the mass of the water-temple seaweed polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition, for example, can be 0.05%, 0.08%, 0.1%, 0.12%, 0.15%, 0.18%, 0.2%, 0.23%, 0.25%, 0.27%, 0.3%, 0.33%, 0.35%, 0.38%, 0.4%, 0.42%, 0.45%, 0.47%, 0.5%, but is not limited to the listed values, and other values within the scope of the present document are also applicable.

[0043] The content of the water-temple seaweed polysaccharide in the anti-wrinkle composition not only affects the stability of the anti-wrinkle composition, but also affects the moisturizing, anti-wrinkle and prevention of ultraviolet damage effects of the anti-wrinkle composition. If the content of the water-temple seaweed polysaccharide is too low, the combination sites of the water-temple seaweed polysaccharide and the sea tangle extract are few, so that the moisturizing, anti-wrinkle and prevention of ultraviolet damage effects of the anti-wrinkle composition are reduced. If the content of the water-temple seaweed polysaccharide is too high, part of the insoluble substances appear in the anti-wrinkle composition, resulting in the reduction of the stability, moisturizing, anti-wrinkle and prevention of ultraviolet damage effects of the anti-wrinkle composition. When the mass of the water-temple seaweed polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition, the stability, moisturizing, anti-wrinkle and prevention of ultraviolet damage effects of the anti-wrinkle composition are high.

[0044] In one embodiment, the mass ratio of the water-temple seaweed polysaccharide and the Gigartina extract is 1:(1-9), for example, can be 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5, 5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, but not limited to the listed values, other values within the scope of the present text are also applicable.

[0045] Compared with the mass ratio of the water-temple seaweed polysaccharide and the Gigartina extract outside the range of 1:(1-9), when the mass ratio of the water-temple seaweed polysaccharide and the Gigartina extract is within the range of 1:(1-9), the moisturizing and anti-wrinkle effects of the obtained anti-wrinkle composition are increased by 5-10%.

[0046] In one embodiment, the weight average molecular weight of the water-temple seaweed polysaccharide is (1.2-2.0) x 10 7 g / mol; for example, can be 1.2 x 10 7 g / mol, 1.4 x 10 7 g / mol, 1.6 x 10 7 g / mol, 1.8 x 10 7 g / mol, 2.0 x 10 7 g / mol, but not limited to the listed values, other values within the scope of the present text are also applicable.

[0047] In one embodiment, the weight average molecular weight of the Gigartina extract is (4.5-5.5) x 10 4 g / mol, for example, can be but not limited to 4.5 x 10 4 g / mol, 4.7 x 10 4 g / mol, 4.9 x 10 4 g / mol, 5.1 x 10 4 g / mol, 5.3 x 10 4 g / mol, 5.5 x 10 4 g / mol.

[0048] In one embodiment, the mass percentage content of the butanediol is less than 77%, preferably less than 65%, further preferably less than 55%, more preferably 25-30%, based on 100% of the mass percentage content of the anti-wrinkle composition.

[0049] The mass percentage content of butanediol in the anti-wrinkle composition affects the stability and preservative effect of the anti-wrinkle composition. The anti-wrinkle composition of the present disclosure does not contain a preservative, butanediol can act as a preservative on the one hand and as a solvent for the anti-wrinkle composition on the other hand. If the mass percentage content of butanediol is too low, it will lead to a decrease in the preservative effect of the anti-wrinkle composition, that is, the service life of the anti-wrinkle composition is greatly shortened. If the mass percentage content of butanediol is too high, it will cause the polysaccharide in the anti-wrinkle composition to precipitate, thereby causing the stability of the anti-wrinkle composition to decrease. The inventors have found that when the mass percentage content of butanediol is within the preferred range, an anti-wrinkle composition with good stability and preservative effect can be obtained.

[0050] In one embodiment, the total sugar content of the anti-wrinkle composition is 0.5-0.7%, for example, it can be 0.5%, 0.52%, 0.55%, 0.57%, 0.6%, 0.63%, 0.65%, 0.68%, 0.7%, but is not limited to the listed values, other values within the scope of the present disclosure are also applicable.

[0051] In one embodiment, the average molecular weight of the polysaccharide in the anti-wrinkle composition is 7.8-8.2 MDa.

[0052] The anti-wrinkle composition of the present disclosure has a high molecular weight, and the anti-wrinkle composition with a high molecular weight has good effects of moisturizing, anti-wrinkling, and preventing ultraviolet damage. Those skilled in the art know that high molecular weight substances are difficult to dissolve, and the anti-wrinkle composition of the present disclosure contains a variety of sugar unit structures, and the non-homogeneity of the various sugar unit structures makes the solubility of the anti-wrinkle composition high.

[0053] The present disclosure also relates to the extraction steps of water temple seaweed polysaccharide and sea moss extract.

[0054] In one embodiment, the plant component (plant material or plant furnish) such as stems, chips, leaves, fines, dust, and / or shorts is initially mixed with a solvent (e.g., water and / or other compounds) at an elevated temperature. For example, a variety of water-miscible solvents (e.g., alcohols such as ethanol) can be combined with water to form a water solvent. In some cases, the water solvent can have a water content greater than 50% by weight of the solvent. In one embodiment, the water content is at least about 70%, or at least about 80%, or at least about 90%, or about 100% by weight of the solvent. Deionized water, distilled water, or tap water can be used. The amount of solvent in the slurry can vary widely, but typically the amount added is about 75% to about 99% by weight of the slurry. However, the amount of solvent can vary depending on the nature of the solvent, the temperature at which the extraction is performed, and the type of plant component.

[0055] After the solvent / plant ingredient mixture is formed, some or all of the soluble extract fraction of the ingredient mixture can optionally be separated (e.g., extracted) from the mixture. If desired, the aqueous solvent / plant ingredient mixture can be agitated during the extraction process by stirring, shaking, or other means of mixing the mixture, thereby increasing the rate of extraction. Typically, the extraction is carried out for about 0.5 hours to about 6 hours.

[0056] Prior to the extraction step, an optional grinding or cutting step can be used to break up the plant or plant parts and thus disrupt the cell walls of the plant. Once separated from the insoluble residue fraction of the plant solution, the soluble extract fraction can optionally be concentrated using any known type of concentrator (e.g., a vacuum evaporator). In one embodiment, the soluble component can be highly concentrated. Additionally, the concentrated or non-concentrated soluble extract fraction can be used in any desired manner.

[0057] In one embodiment, the extraction can also be carried out by means other than using hot water, i.e., by extraction with supercritical gases (e.g., carbon dioxide) or by using solvents such as ethanol, hexane, acetone, R134a (1,1,1,2-tetrafluoroethane), carbon dioxide, and hydrofluorocarbons. In one embodiment, the extraction can be carried out by using at least one solvent at room temperature and atmospheric pressure. The extraction can also be carried out using a mixture of different solvents. In another embodiment, the extraction can be carried out using at least one solvent (e.g., R134a or carbon dioxide) at different temperatures and different pressures and in different states (liquid or gaseous). For example, the extraction can be carried out using solvents in a liquid state (e.g., solvents that are volatile or non-volatile at room temperature), in a subcritical state (e.g., water at temperatures above 100°C and pressures above 1 bar), or in a supercritical state (e.g., carbon dioxide at temperatures above 31°C and pressures above 73 bar).

[0058] In conjunction with the present disclosure, processing aids can be used to improve the extraction, such as pH adjusters (e.g., NaOH or organic acids), microwaves, pressure, ultrasound, enzymes (e.g., proteases, amylases, cellulases, and / or pectinases). When reference is made herein to "extraction," the term includes the foregoing alternative means of extraction. The extraction used in conjunction with the present disclosure can be carried out in a continuous manner or in a discontinuous manner.

[0059] In one embodiment, the extraction and / or pressing can be carried out using at least a portion of fresh, frozen, or dried plant material selected from one or more of roots, stems, barks, trunks, leaves, foliage, fruits, flowers, seeds, or tree bark.

[0060] The soluble fraction (plant extract) and the insoluble fraction (solid plant particles) can be separated by separating the liquid phase from the solid phase, for example by filtration (with or without pressure), by centrifugation or other methods commonly used in the laboratory and known to the skilled person.

[0061] The skilled person can use means known in the art to mix the water-soluble polysaccharide from Porphyra haitanensis, the extract from Gigartina tenella, butanediol and water uniformly, such as mechanical stirring, homogenization, or ultrasonic and the like. After mixing, a uniform and stable anti-wrinkle composition is obtained, which allows the effective components to be mixed sufficiently, and is conducive to the full release of active ingredients.

[0062] In one embodiment, the preparation method of the water-soluble polysaccharide from Porphyra haitanensis is as follows:

[0063] (1) removing water-soluble pigments from the water-soluble polysaccharide from Porphyra haitanensis by freezing and thawing, and then removing fat-soluble pigments from the water-soluble polysaccharide from Porphyra haitanensis by sodium hypochlorite or activated carbon to obtain decolorized solids;

[0064] (2) dissolving the decolorized solids obtained in step (1) with a sodium hydroxide solution, then adjusting the pH, filtering, washing, and drying to obtain the water-soluble polysaccharide from Porphyra haitanensis.

[0065] Specifically, the number of freezing and thawing is 2-5 times.

[0066] Specifically, the mass percentage of sodium hydroxide in the aqueous sodium hydroxide solution is 10%-18%.

[0067] Specifically, the end point of adjusting the pH is adjusting the pH to 6.0-7.0.

[0068] Specifically, the solvent for washing is water and isopropyl alcohol.

[0069] Specifically, the drying has no specific way, for example, it can be air drying, oven drying, freeze drying, etc.

[0070] In one embodiment, the preparation method of the extract from Gigartina tenella is as follows:

[0071] After grinding and defatting the Gigartina tenella at room temperature, the first product obtained is stirred with water, then filtered, washed, and dried to obtain the extract from Gigartina tenella.

[0072] Specifically, before grinding and defatting, the Gigartina tenella needs to be pretreated, and the pretreatment is cleaning and drying. The pretreatment can remove impurities such as sand and dust on the Gigartina tenella.

[0073] Specifically, the defatting step is to place the crushed seaweed powder in ethanol for reflux extraction for 2-3 hours to remove the fat components in the seaweed; the defatted seaweed powder is centrifuged and dried at 50-65°C to constant weight.

[0074] Specifically, the mass ratio of the first product to water is 1:5-20.

[0075] Specifically, the solvent for the washing is water and isopropyl alcohol.

[0076] In a second aspect, the present disclosure provides use of the anti-wrinkle composition in the preparation of a cosmetic product.

[0077] In a third aspect, the present disclosure provides a cosmetic product comprising the anti-wrinkle composition.

[0078] In one embodiment, the anti-wrinkle composition is present in the cosmetic product at a weight percentage of 0.5-10%, for example, can be 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, but is not limited to the listed values, other values within the range herein are also applicable.

[0079] The addition of the anti-wrinkle composition in a specific ratio in the cosmetic product can bring good anti-wrinkle effect to the cosmetic product. When the cosmetic product of the present application contains less than 0.5% by weight of the anti-wrinkle composition of the present application, it cannot be expected to sufficiently enhance skin elasticity or improve wrinkles; or moisturize the skin, and when more than 10% by weight is contained, it can cause an allergic reaction or other undesirable reaction or a problem in skin safety.

[0080] In one embodiment, the cosmetic product can be in the form of a general emulsifier type and a soluble agent type. For example, it can be in the form of a cosmetic water such as a skin softener or a nutrient cosmetic water, an emulsion such as a facial lotion, a body lotion, a cream such as a nutrient cream, a moisturizing cream, an eye cream, a serum, a cosmetic ointment, a balm, a spray, a gel, a mask, a sunscreen, a primer, a foundation in liquid, solid or spray type, a powder, a makeup remover such as a cleansing cream, a cleansing lotion, a cleansing oil, a cleanser such as a cleansing foam, a soap, a shower gel, etc.

[0081] The cosmetic also comprises at least one cosmetically acceptable adjuvant; for example fatty substances, humectants, emollients, conditioning agents, softening agents, thickening agents, organic solvents, suspending agents, solubilizing agents, concentrating and gelling agents, antioxidants, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non-ionic emulsifiers, fillers, metal ion blocking and chelating agents, preservatives, vitamins, blocking agents, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics.

[0082] The above-mentioned ingredients contained in the composition according to the present application can each preferably be contained in the composition of the present application within the range of not more than the maximum usage amount specified in the regulations of the respective governments.

[0083] In one embodiment, the cosmetic is a cream, and the cream includes the following components in weight percentage: anti-wrinkle composition 0.5-10%, p-hydroxyacetophenone 0.3-1%, butylene glycol 1-6%, xanthan gum 0.05-0.3%, emulsifier 0.5-1.5%, polydimethylsiloxane 0.5-10%, caprylic / capric triglyceride 0.5-10%, 1,2-hexanediol 0.3-1%, deionized water balance; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, trideceth-6 in a mass ratio of 62:34:4.

[0084] In one embodiment, the cosmetic is a gel, and the gel includes the following components in weight percentage: anti-wrinkle composition 0.5-10%, p-hydroxyacetophenone 0.3-1.0%, emulsifier 0.5-1.5%, 1,2-hexanediol 0.3-1.0%, deionized water balance; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, trideceth-6 in a mass ratio of 62:34:4.

[0085] In one embodiment, the cosmetic is a cream, and the cream comprises the following components in the following weight percentages: anti-wrinkle composition 0.5-10%, p-hydroxyacetophenone 0.5-1%, butylene glycol 0.5-5%, xanthan gum 0.05-0.2%, sodium stearoyl glutamate 0.02-0.5%, acryloyldimethyltaurine ammonium / VP copolymer 0.3-1.0%, mixture of cetyl stearyl alcohol and sorbitan olivate 1.0-2.5%, cetyl stearyl alcohol 0.5-3.0%, caprylic / capric triglyceride 0.5-5.0%, hydrogenated polydecene 0.5-5.0%, dimethicone with a kinematic viscosity of 100 cSt 0.5-5.0%, dimethicone with a kinematic viscosity of 5 cSt 0.5-5.0%, limnanthes alba (meadowfoam) seed oil 0.5-5.0%, 1,2-hexanediol 0.5-1.0%, deionized water q.s.; wherein the mass ratio of cetyl stearyl alcohol and sorbitan olivate is 5-7:3-5.

[0086] Cosmetic "comprises" means that it can include other ingredients in addition to the ingredients listed, which give the cosmetic different properties. In addition, "comprises" in the present disclosure can be replaced by the closed "consists of" or "consists".

[0087] It should be noted that the balance is water, which means that in the eye cream raw materials of the present application, in addition to the anti-wrinkle composition, p-hydroxyacetophenone, butylene glycol, xanthan gum, emulsifier, polydimethylsiloxane, caprylic / capric triglyceride, 1,2-hexanediol, and optionally other components, the weight percentage content of the anti-wrinkle composition, p-hydroxyacetophenone, butylene glycol, xanthan gum, emulsifier, polydimethylsiloxane, caprylic / capric triglyceride, 1,2-hexanediol, and optionally other components is 100%;

[0088] Or it means that in the jelly raw materials of the present application, in addition to the anti-wrinkle composition, p-hydroxyacetophenone, emulsifier, 1,2-hexanediol, and optionally other components, the weight percentage content of the anti-wrinkle composition, p-hydroxyacetophenone, emulsifier, 1,2-hexanediol, and optionally other components is 100%;

[0089] Or it means that in the cream raw materials of the present application, in addition to the anti-wrinkle composition, p-hydroxyacetophenone, butylene glycol, xanthan gum, sodium stearoyl glutamate, acryloyldimethyltaurine ammonium / VP copolymer, mixture of cetyl stearyl alcohol and sorbitan olivate, cetyl stearyl alcohol, caprylic / capric triglyceride, hydrogenated polydecene, dimethicone with a kinematic viscosity of 100 cSt, dimethicone with a kinematic viscosity of 5 cSt, limnanthes alba (meadowfoam) seed oil, 1,2-hexanediol, and optionally other components, the balance is water.

[0090] Another aspect of the present disclosure also relates to a preparation method of the gel, comprising the following steps: adding deionized water, p-hydroxyacetophenone and emulsifier into a container, heating to 75-80℃ and mixing uniformly, then homogenizing at a speed of 8000-100000 rpm for 2-3 min, then cooling to 45℃, and then adding anti-wrinkle composition and 1,2-hexanediol in sequence and stirring uniformly.

[0091] Another aspect of the present disclosure also relates to a preparation method of the eye cream, comprising the following steps:

[0092] (1) adding deionized water, p-hydroxyacetophenone, butanediol and xanthan gum into a container, heating to 75-80℃ and mixing uniformly to obtain phase A, and keeping warm for standby;

[0093] (2) adding polydimethylsiloxane and caprylic / capric triglyceride into a container, heating to 75-80℃ and mixing uniformly to obtain phase B, and keeping warm for standby;

[0094] (3) adding the phase B obtained in step (2) and emulsifier into the phase A obtained in step (1), homogenizing at a speed of 8000-100000 rpm for 2-3 min, then cooling to 45℃, and then adding anti-wrinkle composition and 1,2-hexanediol in sequence and stirring uniformly.

[0095] Another aspect of the present disclosure also relates to a preparation method of the face cream, comprising the following steps:

[0096] (1) adding deionized water, p-hydroxyacetophenone, butanediol, xanthan gum and sodium stearoyl glutamate into a container, heating to 80-85℃ and mixing uniformly to obtain phase A, and keeping warm for standby;

[0097] (2) adding a mixture of cetyl stearyl alcohol and sorbitan olivate, cetyl stearyl alcohol, caprylic / capric triglyceride, hydrogenated polydecene, polydimethylsiloxane with a kinematic viscosity of 100 cSt, polydimethylsiloxane with a kinematic viscosity of 5 cSt and limnanthes alba seed oil into a container, heating to 80-85℃ and mixing uniformly, then adding acryloyldimethyltaurine ammonium / VP copolymer and mixing uniformly to obtain phase B, and keeping warm for standby;

[0098] (3) adding the phase B obtained in step (2) into the phase A obtained in step (1), homogenizing at a speed of 8000-100000 rpm for 2-3 min, then cooling to 45℃, and then adding anti-wrinkle composition and 1,2-hexanediol in sequence and stirring uniformly.

[0099] In order to further understand the present application, the anti-wrinkle composition, the preparation method and the effect thereof will be further described in detail below in combination with specific examples.

[0100] The water temple seaweed polysaccharide is self-made, and the preparation method thereof is as follows:

[0101] (1) The water-soluble pigment of the water temple seaweed polysaccharide is removed by freezing and thawing, and then the fat-soluble pigment of the water temple seaweed polysaccharide is removed by hypochlorous acid to obtain a decolorized solid;

[0102] (2) The decolorized solid obtained in step (1) is dissolved with a sodium hydroxide solution with a mass percentage of 10%, and the mass ratio of the sodium hydroxide solution to the decolorized solid is 20:1, and then the pH is adjusted to pH = 6.0-6.5, filtered, the obtained filter is washed with water for 3 times, and dried to obtain the water temple seaweed polysaccharide, and the molecular weight of the water temple seaweed polysaccharide is 1.6 x 10 7 g / mol.

[0103] The sea moss extract is self-made, and the preparation method thereof is as follows: fresh sea moss is washed and dried, and then ground and defatted at room temperature to obtain a first product, the first product and water are uniformly mixed at a mass ratio of 1:10, stirred at room temperature for 24 h, filtered, the obtained filter is washed with water for 3 times, and dried to obtain the sea moss extract, and the weight average molecular weight of the sea moss extract is 4.9 x 10 4 g / mol.

[0104] Examples and comparative examples

[0105] The components and weight parts of the anti-wrinkle composition of the examples and comparative examples are shown in Table 1, and the preparation method of the anti-wrinkle composition of the examples and comparative examples comprises the following steps: opening the stirring, under the condition of a rotating speed of 800 rpm, each component is added into a container according to the weight parts in Table 1, and stirred for 30 min to obtain the anti-wrinkle composition.

[0106] Table 1

[0107] Test example 1

[0108] The stability of the anti-wrinkle composition obtained in each example and comparative example is tested in this test example.

[0109] Test method: the anti-wrinkle composition obtained in each example and comparative example is respectively placed under the conditions of-18℃, 5℃, RT, 40℃, 50℃, UV and-10 / 45℃ cycle, and at different time points, after being taken out and restored to room temperature, the sensory property evaluation and the physical and chemical property determination are carried out, and the judgment rules are as follows: o: normal; x: abnormal; +: discoloring degree.

[0110] General judgment requirements: 1, UV requires D14 Stable, less than 14 days is judged as unqualified; 2, temperature stability requirement D 1M Stable, less than D 1M is judged as unqualified.

[0111] The color test results of the anti-wrinkle compositions under 50°C and UV conditions are shown in Table 2.

[0112] Table 2

[0113] It is found through the test that the appearance of Examples 1-17 and Comparative Examples 3-8 is transparent and / or translucent liquid, and the appearance of Examples 1-17 and Comparative Examples 3-8 has no obvious change after being placed under-18°C, 5°C, RT, 40°C, 50°C, UV and-10 / 45°C cycle conditions for 3 months; the appearance of Comparative Example 1 is a liquid containing flocculation, and the flocculation obviously increases after being placed under-18°C, 5°C, RT, 40°C, 50°C, UV and-10 / 45°C cycle conditions for 3 months; the appearance of Comparative Example 2 is a liquid containing insoluble matter, and the appearance of Comparative Example 2 has no obvious change after being placed under-18°C, 5°C, RT, 40°C, 50°C, UV and-10 / 45°C cycle conditions for 3 months.

[0114] The odor of the anti-wrinkle compositions of Examples 1-17 and Comparative Examples 1-8 has no obvious change after being placed under-18°C, 5°C, RT, 40°C, 50°C, UV and-10 / 45°C cycle conditions for 3 months.

[0115] The color of the anti-wrinkle compositions of Examples 1-17 and Comparative Examples 1-8 has no obvious change after being placed under-18°C, 5°C, RT, 40°C and-10 / 45°C cycle conditions for 3 months; the color change of the anti-wrinkle compositions of Examples 1-17 and Comparative Examples 1-8 at different time points after being placed under 50°C and UV conditions is shown in Table 2, from which it can be seen that the color change of the anti-wrinkle compositions of the present disclosure all meet the stability requirement, while the content of butanediol in Comparative Example 1 is too high, and it starts to turn brown after being placed under UV conditions for 2 months, indicating that the stability of the anti-wrinkle composition of Comparative Example 2 is poor; the content of porphyra hair-seaweed polysaccharide in Comparative Example 2 is too high, and it starts to turn brown after being placed under UV conditions for 7 days, indicating that the stability of the anti-wrinkle composition of Comparative Example 2 is poor.

[0116] Figure 1 is a state diagram of the anti-wrinkle composition of Comparative Example 2 after being placed under different conditions for different time, from which it can be seen that the anti-wrinkle composition of Comparative Example 2 starts to turn brown after being placed under UV conditions for 7 days, and the color further deepens with the extension of the placement time; the color turns yellow after being placed under 50°C conditions for 1 month.

[0117] Test Example 2

[0118] This test example tests the sugar unit structure contained in the anti-wrinkle compositions of Examples 15-17 and Comparative Examples 3-8.

[0119] This test example identifies the sugar unit structure contained in the anti-wrinkle compositions of Examples 15-17 and Comparative Examples 3-8 by determining the interaction of the anti-wrinkle compositions with 25 lectins, and the ability of the samples to be recognized by the lectins can be evaluated by obtaining the sugar profile of different samples, and the results are shown in Figures 2-4 and Table 3.

[0120] Table 3 Inhibition rate of the anti-wrinkle compositions of Examples 15-17 and Comparative Examples 3-8 to each lectin (unit: %) Note: Fuc: fucose, Glc: glucose, Gal: galactose, Man: mannose, GalNAc: n-acetylgalactosamine, GlcNAc: n-acetylglucosamine, GlcU: glucuronic acid, NeuAc: neuraminic acid, Rha: rhamnose, Xyl: xylitol, βglucane: β-glucan.

[0121] Figure 2 is the inhibition of the anti-wrinkle compositions of Comparative Examples 3-5 to different lectins, Figure 3 is the inhibition of the anti-wrinkle compositions of Comparative Examples 6-8 to different lectins, and Figure 4 is the inhibition of the anti-wrinkle compositions of Examples 15-17 to different lectins. From the experimental data of Figures 2-4 and Table 3, it can be seen that the anti-wrinkle compositions containing only water fern nori polysaccharide or sea moss extract have no or almost no inhibition to DBA and AIA at low concentrations, while the anti-wrinkle compositions containing both water fern nori polysaccharide and sea moss extract can synergistically enhance the effect of the anti-wrinkle compositions on lectins such as DBA, AIA, Langerin, Gal3, etc.; indicating that the water fern nori polysaccharide and sea moss extract in the anti-wrinkle compositions of the present application have a synergistic effect.

[0122] Test Example 3

[0123] This test example tests the effect of the anti-wrinkle composition of Example 16 on preventing UV damage.

[0124] 1. Materials

[0125] HS68 (CRL-1635) human dermal fibroblasts were obtained from American Type Culture Collection (ATCC); DMEM medium, penicillin, streptomycin, cinnamyl alcohol, HEPES, phosphatase inhibitor cocktail and Triton X-100 were purchased from Nacalai Tesque (Kyoto, Japan); ethylenediaminetetraacetic acid (EDTA), skim milk, Tween 20 and dithiothreitol (DTT) were purchased from Wako Pure Chemical (Osaka, Japan); IGF-1 was purchased from Abeam (Tokyo, Japan); Hybond-ECL membrane was from GE Healthcare UK, Ltd (Buckinghamshire, UK), protease inhibitor cocktail was from Roche Applied Science (Mannheim, Germany); fetal bovine serum (FBS) was purchased from Invitrogen (Carlsbad, CA, USA); EZ-ECL chemiluminescence detection kit for chemiluminescence detection was from Biolog Israel (Kibbutz Beit Haemek, Israel), anti-wrinkle composition of Example 16.

[0126] 2. Cell culture

[0127] HS68 (CRL-1635) human dermal fibroblasts were cultured in DMEM medium containing 10% (V / V) FBS, 100 U / mL penicillin, 100 μg / mL streptomycin at 37°C in 5% CO2.

[0128] 3. Experimental method

[0129] 3.1 Toxic effect of anti-wrinkle composition on HS68 cells

[0130] Normal control group and different dose irradiation groups (irradiation groups were further divided into groups irradiated with 0.01, 0.1, 1, 5, 10 μg / mL anti-wrinkle composition, respectively) were set in 96-well plates, and logarithmic growth phase HS68 cells were taken at 1 x 10 4HS68 cells were seeded at a density of 1 x 105cells / well in 6-well plates, with 6 experimental wells per group. The edge wells were filled with sterile PBS. After 24 h incubation at 37°C in 5% CO2, the cells were treated with the anti-wrinkle composition at the concentrations described above. After 24 h, the medium was removed and the cells were washed with PBS. The cells were then treated with UVB using an ultraviolet crosslinker (UVP, Upland, CA, USA) at a dose of 30 mJ / cm2. After the treatment, the MTT test was performed. The MTT test was performed as described in “3.1”.

[0131] 3.2 Effect of the anti-wrinkle composition on HS68 cells induced by UVB

[0132] HS68 cells were seeded at a density of 1 x 105cells / well in 96-well plates, with 6 experimental wells per group. The edge wells were filled with sterile PBS. After 24 h incubation at 37°C in 5% CO2, the cells were treated with the anti-wrinkle composition at the concentrations described above. After 24 h, the medium was removed and the cells were washed with PBS. The cells were then treated with UVB using an ultraviolet crosslinker (UVP, Upland, CA, USA) at a dose of 30 mJ / cm2. After the treatment, the MTT test was performed. The MTT test was performed as described in “3.1”. 4 HS68 cells were seeded at a density of 1 x 105cells / well in 96-well plates, with 6 experimental wells per group. The edge wells were filled with sterile PBS. After 24 h incubation at 37°C in 5% CO2, the cells were treated with the anti-wrinkle composition at the concentrations described above. After 24 h, the medium was removed and the cells were washed with PBS. The cells were then treated with UVB using an ultraviolet crosslinker (UVP, Upland, CA, USA) at a dose of 30 mJ / cm2. After the treatment, the MTT test was performed. The MTT test was performed as described in “3.1”. 2

[0133] 3.3 DCF fluorescence assay of ROS levels in HS68 cells

[0134] After the HS68 cells were seeded and treated according to the method of “3.2”, the cells were stained with 10 μM DCFH-DA and analyzed using an Axiovert 200M fluorescence spectrophotometer (Zeiss, Oberkochen, Germany). The ROS levels between the cells were shown by the fluorescence of DCFH-DA, which was measured on a microplate reader (Infinite M200 Pro, Tecan, Switzerland) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm.

[0135] 3.4 SDS-PAGE and immunoblotting analysis

[0136] ​After inoculation and treatment of HS68 cells according to the method of "3.2", the cell suspension of each group was centrifuged to remove the supernatant, and then 100 μL of cell lysis solution (protease inhibitor: 1 x RIPA buffer (10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% deoxycholic acid sodium, 1% Triton X-100, 0.1% SDS and 1 mM EDTA)) was added to lyse the cells, and then ultrasonic was performed for 10 seconds. The protein concentration of each lysis solution was determined by BCA protein assay reagent.

[0137] After reduction with 50 mM DTT, the proteins were run on 8% SDS-polyacrylamide gel, transferred to nitrocellulose membrane, incubated with 1 x TBST buffer solution containing 5% skim milk at room temperature for 1 h, washed 3 times in 1 x TBST buffer solution for 10 minutes. The membrane containing the target protein was added with the corresponding primary antibody solution and incubated on a shaking table at 4°C overnight. After overnight incubation, the membrane was washed 3 times with 1 x TBST at room temperature for 20-30 min each time, and the corresponding secondary antibody solution was added, and the membrane was incubated at room temperature for 2 h in the dark on a shaking table, and then washed 3 times with 1 x TBST at room temperature for 30 min each time. The membrane was incubated with horseradish peroxidase (HRP)-coupled secondary antibody at room temperature for 1 hour.

[0138] Antibodies against MMP1, HAS1, COL1A1, COL1A2 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell signaling Technologies (Danver, USA). Protein levels of MMP-1, HAS1, COLa1 and COLa2 were determined by chemiluminescence imaging system (Bio-Rad) scanning the membrane.

[0139] 3.5 Real-time qPCR quantification

[0140] A negative control group (without anti-wrinkle composition and UVB treatment) and different dose irradiation groups (the irradiation groups were further divided into groups treated with 0, 0.01, 0.1, 1, 5 μg / mL anti-wrinkle composition, respectively) were set up in a 6-well plate. Logarithmic growth phase HS68 cells were cultured at 5% CO2, 37°C for 24 h, and then the anti-wrinkle composition of the above concentrations was added to the irradiation groups at 50 μL per well, and the cells were cultured for another 24 h. After the culture medium was aspirated and the cells were washed with PBS, the cells were treated with UVB using a UV crosslinker (UVP, Upland, CA, USA) at a dose of 30 mJ / cm 2After the treatment, total RNA was collected using the RNeasy Mini RNA Isolation Kit (Qiagen, Valencia, CA, USA). The RNA was eluted into RNase-free water, quantified by UV absorbance, and aliquoted at -80°C until use. Reverse transcription (RT)-PCR was performed on an Applied Biosystems 7500 Real-Time PCR System using the following amplification conditions: preincubation at 95°C for 10 min, 40 cycles of 95°C for 10 s, 60°C for 15 s, and 72°C for 15 s. Gene expression levels were normalized to GAPDH and calculated using the comparative ΔΔCT method.

[0141] 3.6 Statistical methods

[0142] All experiments were performed in triplicate, and the results are expressed as the mean ± standard deviation (SD). Statistical analysis was performed using SPSS software (SPSS, Inc., Tokyo, Japan). Student's t-test, analysis of variance, and appropriate post-hoc tests (Tukey's) were performed according to the nature and distribution of the data. Differences were considered statistically significant at p < 0.05. The results are shown in Tables 4-7 and Figure 5.

[0143] 4. Results and analysis

[0144] 4.1 Toxic effects of anti-wrinkle compositions on HS68 cells

[0145] Table 4 Toxic effects of anti-wrinkle compositions at different concentrations on HS68 cells

[0146] As can be seen from Table 4, the anti-wrinkle compositions at different concentrations had no significant toxic effects on the cells after 24 h of intervention. Compared with the normal control group, no significant changes in cell survival rate were observed with increasing concentrations of anti-wrinkle compositions, and the differences were not statistically significant (P > 0.05). Therefore, the anti-wrinkle compositions at different concentrations had no significant cytotoxic effects on the viability of HS68 cells, and had little effect on the survival rate of HS68 cells.

[0147] 4.2 Effects of anti-wrinkle compositions on the survival of UVB-induced HS68 cells

[0148] Table 5 Effects of anti-wrinkle compositions at different concentrations on the survival of UVB-induced HS68 cells

[0149] As shown in Table 5, the survival rate of HS68 cells after UVB irradiation was 73.43%, and the protective effect on HS68 cells increased in a dose-dependent manner when the concentration of the anti-wrinkle composition was in the range of 0.01-0.1 μg / mL, and the anti-wrinkle composition had a good proliferative effect on HS68 cells when the concentration of the anti-wrinkle composition was 0.1-5 μg / mL, which was significantly different (P < 0.01) compared with the group without anti-wrinkle composition treatment.

[0150] 4.3 Effect of Anti-wrinkle Composition on ROS Level in UVB-induced HS68 Cells

[0151] Table 6 Effect of Anti-wrinkle Composition of Different Concentrations on ROS Level in UVB-induced HS68 Cells

[0152] As shown in Table 6, UVB irradiation increased the ROS content in HS68 cells to 175.9%, and the ROS scavenging effect of the anti-wrinkle composition on UVB damage gradually increased with the increase of the concentration of the anti-wrinkle composition, and was dose-dependent. Among them, 0.01-5 μg / mL of the anti-wrinkle composition was used to act on the UVB-irradiated HS68 cells, and the intracellular ROS was significantly lower than that without anti-wrinkle composition treatment, and decreased more with the increase of the dose (P < 0.01).

[0153] 4.4 Effect of Anti-wrinkle Composition on Expression of Related Proteins in UVB-induced HS68 Cells

[0154] Table 7 Effect of Anti-wrinkle Composition of Different Concentrations on Expression of Related Proteins in UVB-induced HS68 Cells

[0155] (1) Anti-wrinkle Composition Inhibits UVB-induced Production of MMP1 in HS68 Cells

[0156] As shown in Table 7, UVB irradiation promotes DNA damage in skin cells by producing oxidative stress and ROS, leading to further structural and functional damage, and the increase of MMP1 expression caused by UVB irradiation is one of the key factors leading to the degradation of ECM proteins; 0.01-5 μg / mL of the anti-wrinkle composition can reduce the expression of MMP1, and the effect is dose-dependent; compared with the irradiation group without anti-wrinkle composition intervention, even with the lowest dose of 0.01 μg / mL of the anti-wrinkle composition, the decrease of MMP1 production has statistical significance (P < 0.01).

[0157] (2) Anti-wrinkle Composition Promotes UVB-induced Production of HAS1 in HS68 Cells

[0158] Hyaluronic acid synthase 1 (HAS1) is an enzyme that plays a major role in the production of hyaluronic acid in the skin; the experimental results are shown in Table 7, and UVB irradiation significantly reduces HAS1 in HS68 cells, and the anti-wrinkle composition can increase the UVB-induced production of HAS1, and compared with the irradiation group without intervention of the anti-wrinkle composition, the intervention of 0.01-5 μg / mL of the anti-wrinkle composition has statistical significance (P<0.01) in the production of HAS1.

[0159] (2) Anti-wrinkle composition promotes the production of COL1A1 and COL1A2 of UVB-induced HS68 cells

[0160] Collagen is an important ECM protein, and type 1 collagen accounts for about 80% of dermal collagen. Type 1 collagen is composed of two α1(I) chains and one α2(I) chain, which are encoded by two genes COL1A1 and COL1A2. The protein expression levels of COL1A1 and COL1A2 were evaluated by immunoblotting and Q-PCR, and the experimental results are shown in Figure 5 and Table 7, and UVB irradiation reduces the production and expression of type 1 collagen subunits, and the pretreatment of the anti-wrinkle composition at all test concentrations effectively resists this reduction, and compared with the irradiation group without intervention of the anti-wrinkle composition, the intervention of 0.01-5 μg / mL of the anti-wrinkle composition has statistical significance (P<0.01) in the production of COL1A1 and COL1A2.

[0161] In summary, the anti-wrinkle composition of the present disclosure can effectively prevent ultraviolet damage.

[0162] Application Example 1

[0163] The present application provides a gel, which comprises the following components by weight percentage: 1% of the anti-wrinkle composition of Example 6, 0.5% of p-hydroxyacetophenone, 0.8% of emulsifier, 0.5% of 1,2-hexanediol, and the balance of deionized water; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate, and tridecyl alcohol polyether-6 in a mass ratio of 62:34:4.

[0164] The present application also provides a preparation method of the gel, which comprises the following steps:

[0165] Deionized water, p-hydroxyacetophenone, and emulsifier are added to a container, heated to 78°C, mixed uniformly, then homogenized at a speed of 9000 rpm for 3 min, then cooled to 45°C, and then the anti-wrinkle composition of Example 6 and 1,2-hexanediol are added in sequence, and stirred uniformly.

[0166] Application Example 2

[0167] The application example provides an eye cream, which contains the following components in percentage by weight: 5% of the anti-wrinkle composition of example 6, 0.5% of p-hydroxyacetophenone, 3% of butanediol, 0.2% of xanthan gum, 1% of emulsifier, 5% of dimethicone, 3% of caprylic / capric triglyceride, 0.5% of 1,2-hexanediol, and deionized water in balance; wherein the emulsifier is composed of sodium polyacrylate, ethylhexyl stearate and tridecyl alcohol polyether-6 in a mass ratio of 62:34:4.

[0168] The application example also provides a preparation method of the eye cream, which comprises the following steps:

[0169] (1) adding deionized water, p-hydroxyacetophenone, butanediol and xanthan gum into a container, uniformly mixing after heating to 78 DEG C, and obtaining phase A for standby;

[0170] (2) adding dimethicone and caprylic / capric triglyceride into a container, uniformly mixing after heating to 78 DEG C, and obtaining phase B for standby;

[0171] (3) adding the phase B obtained in the step (2) and the emulsifier into the phase A obtained in the step (1), homogenizing at a rotating speed of 9000 rpm for 3 min, then cooling to 45 DEG C, and sequentially adding the anti-wrinkle composition of example 6 and 1,2-hexanediol, and uniformly stirring.

[0172] Application example 3

[0173] The application example provides a face cream, which contains the following components in percentage by weight: 1% of anti-wrinkle composition, 0.5% of p-hydroxyacetophenone, 3% of butanediol, 0.12% of xanthan gum, 0.05% of sodium stearoyl glutamate, 0.6% of acryloyldimethyltaurine ammonium / VP copolymer, 1.8% of a mixture of cetyl stearyl alcohol and sorbitan olive acid ester, 2% of cetyl stearyl alcohol, 4% of caprylic / capric triglyceride, 2% of hydrogenated polydecene, 2% of dimethicone with a kinematic viscosity of 100 cSt, 2% of dimethicone with a kinematic viscosity of 5 cSt, 2% of limnanthes alba seed oil, and 1% of 1,2-hexanediol; wherein the mass ratio of cetyl stearyl alcohol and sorbitan olive acid ester is 6:4.

[0174] The application example also provides a preparation method of the face cream, which comprises the following steps:

[0175] (1) adding deionized water, p-hydroxyacetophenone, butanediol, xanthan gum and sodium stearoyl glutamate into a container, uniformly mixing after heating to 82 DEG C, and obtaining phase A for standby;

[0176] (2) A mixture of cetearyl olivate and sorbitan olivate, cetearyl alcohol, caprylic / capric triglyceride, hydrogenated polydecene, dimethicone with kinematic viscosity of 100 cSt, dimethicone with kinematic viscosity of 5 cSt and limnanthes alba (meadowfoam) seed oil were added into a container, heated to 80°C, mixed uniformly, then acrylates / ammonium methacrylate copolymer was added, mixed uniformly to obtain phase B, and kept warm for standby;

[0177] (3) The phase B obtained in step (2) and emulsifier were added into the phase A obtained in step (1), homogenized at a speed of 9000 rpm for 3 min, then cooled to 45°C, the anti-wrinkle composition of example 6 and 1,2-hexanediol were added in turn, and stirred uniformly.

[0178] Comparative application example 1

[0179] The present comparative application example provides a gel, which is different from application example 1 only in that the anti-wrinkle composition of example 6 is replaced by deionized water, i.e. the present comparative application example does not contain the anti-wrinkle composition of example 6.

[0180] The preparation method of the gel in the present comparative application example is the same as that in application example 1.

[0181] Comparative application example 2

[0182] The present comparative application example provides an eye cream, which is different from application example 2 only in that the anti-wrinkle composition of example 6 is replaced by water, i.e. the present comparative application example does not contain the anti-wrinkle composition of example 6.

[0183] The preparation method of the eye cream in the present comparative application example is the same as that in application example 2.

[0184] Comparative application example 3

[0185] The present comparative application example provides a face cream, which is different from application example 3 only in that the anti-wrinkle composition of example 6 is replaced by water, i.e. the present comparative application example does not contain the anti-wrinkle composition of example 6.

[0186] The preparation method of the face cream in the present comparative application example is the same as that in application example 3.

[0187] Test example 4

[0188] The present effect example tests the moisturizing effect of the gels obtained in application example 1 and comparative application example 1.

[0189] Test method: According to QB / T 4256-2011 "Guidelines for evaluating the moisturizing efficacy of cosmetics", 5 volunteers aged 18-65 were included in the test, and all the volunteers signed the informed consent form. Mark the application area and the blank control area on the inner side of the forearm of both hands (size: 3 cm x 3 cm), and the blank control area is not applied with any substance. The test sample is applied in a single application with a dosage of (2.0 ± 0.1) mg / cm 2 The amount of transdermal water loss of the skin before and after the use of the sample was measured by the transdermal water loss measuring instrument VapoMeter, the change rate of transdermal water loss (%) = (data after use-data before use) / data before use x 100%, and the test results are shown in Table 8. From the experimental data in Table 8, it can be seen that the anti-wrinkle composition of the present disclosure has a moisturizing effect.

[0190] Table 8 Change rate of transdermal water loss

[0191] Test Example 5

[0192] This effect example tests the anti-wrinkle effect of the eye cream obtained from application example 2 and comparative application example 2.

[0193] Test method: 9 volunteers aged 35-45 were included in the test, and all the volunteers signed the informed consent form. The volunteers were arranged to use the eye cream obtained from application example 2 and comparative application example 2 on half of the face in the morning and evening respectively once a day for 14 days, and were visited on the 1st day (initial value), 7th day and 14th day. The proportion of wrinkle area to area in the selected area was detected by the skin analysis system CANFIELD VISIA 7, the maximum depth of wrinkle in the selected area (maximum concave depth), the area volume of the selected area where the wrinkles formed a depression (depression volume), and the overall evaluation of the degree of wrinkle depression in the selected area (depression index / depression score) were detected by the skin detector Miravex ANTERA 3D. All tests were conducted after the face was cleaned with a unified cleansing and the test was conducted in a test environment (temperature 20 ± 1 ℃, humidity 50 ± 10%) for 30 min. The data were analyzed by IPP software and ANTERA CS software, and the test results are shown in Table 9 and Figs. 6-7.

[0194] Table 9

[0195] As can be seen from Table 9 and Figs. 6-7, the anti-wrinkle composition can effectively improve the wrinkle depression depth, depression index and depression volume of the eye skin, and slightly improve the area of the eye skin.

[0196] Test Example 6

[0197] The effect example tests the moisturizing and anti-wrinkle effects of the creams obtained in application example 3 and comparative application example 3.

[0198] Test method: 32 volunteers aged 35-55 were enrolled in the test, and all the volunteers signed the informed consent form. The volunteers were arranged to use the creams obtained in application example 3 and comparative application example 3 once a day on half of the face in the morning and evening respectively for 28 days, and were visited on the 1st day (initial value), 14th day and 28th day, and the skin water content was detected by the skin moisture measurement probe Corneometer@CM 825 (Courage & Khazaka, Germany), the TEWL value of the skin was detected by the trans-epidermal water loss probe Vapometer (Delfin, Finland), the skin elasticity was detected by the skin elasticity probe Dual MPA 580 (Courage + Khazaka), the skin color content was detected by the skin color probe Colorimeter CL400, and the improvement of the under-eye fine lines and crow's feet was detected by Visia@CR (Canfield) and PRIMOS, and the crow's feet grade and eye wrinkle grade were evaluated according to the "Atlas of Skin Aging Second Edition Asian Version" through clinical evaluation; all the tests were tested after the face was cleaned with a unified cleansing and sat in the experimental environment (temperature 20±1℃, humidity 50±10%) for 30 min, and the efficacy of the serum was expressed by the improvement rate, the change rate (%) = (data after use-data before use) / data before use x 100%; the Office-EXCEL software was used to process and plot the data, the SPSS 25.0 software was used for single factor analysis of variance (P<0.005), and all the tests were repeated 3 times. The test results are shown in Table 10 and Figs. 8-11. (Canfield) and PRIMOS, and the crow's feet grade and eye wrinkle grade were evaluated according to the "Atlas of Skin Aging Second Edition Asian Version" through clinical evaluation; all the tests were tested after the face was cleaned with a unified cleansing and sat in the experimental environment (temperature 20±1℃, humidity 50±10%) for 30 min, and the efficacy of the serum was expressed by the improvement rate, the change rate (%) = (data after use-data before use) / data before use x 100%; the Office-EXCEL software was used to process and plot the data, the SPSS 25.0 software was used for single factor analysis of variance (P<0.005), and all the tests were repeated 3 times. The test results are shown in Table 10 and Figs. 8-11.

[0199] Table 10

[0200] Figs. 8 and 9 are respectively the change diagrams of the crow's feet of a volunteer before and after using the cream of application example 3; Figs. 10 and 11 are respectively the change diagrams of the under-eye wrinkles of a volunteer before and after using the cream of application example 3. As shown in Table 10 and Figs. 8-11, after using the cream containing the anti-wrinkle composition of the present disclosure, the improvement of the skin water content, the TEWL value of the skin and the skin elasticity can be improved, and the crow's feet and the under-eye wrinkles of the skin can be reduced, which indicates that the anti-wrinkle composition of the present disclosure has excellent moisturizing, water supplementing, skin elasticity improving and anti-wrinkle effects.

[0201] Finally, it should be noted that the above examples are used to illustrate the technical solutions of the present disclosure and not to limit the protection scope of the present disclosure. Although the present disclosure has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present disclosure can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present disclosure.

Claims

1. An anti-wrinkle composition, characterized by comprising, The composition comprises the following components by weight: water 50-600 parts, water-temple nori polysaccharide 0.1-1.5 parts, sea moss extract 0.1-70 parts, butanediol 10-230 parts.

2. The anti-wrinkle composition according to claim 1, wherein The mass of the water-temple nori polysaccharide is 0.05-0.5% of the mass of the anti-wrinkle composition.

3. The anti-wrinkle composition according to claim 1, wherein The mass ratio of the water-temple nori polysaccharide and the sea moss extract is 1:(1-9).

4. The anti-wrinkle composition according to claim 1, wherein The water-front temple seaweed polysaccharide has a weight average molecular weight of (1.2-2.0) x 10 7 g / mol; and / or, the sea moss extract has a weight average molecular weight of (4.5-5.5) x 10 4 g / mol.

5. The anti-wrinkle composition according to claim 1, wherein The mass percentage of the butanediol is less than 77% based on 100% of the mass percentage of the anti-wrinkle composition.

6. The anti-wrinkle composition according to claim 1, wherein The total sugar content of the anti-wrinkle composition is 0.5-0.7%.

7. Use of the anti-wrinkle composition according to any one of claims 1-6 in the preparation of a cosmetic product.

8. A cosmetic product, characterized by, The cosmetic product comprises the anti-wrinkle composition according to any one of claims 1-6.

9. The cosmetic product according to claim 8, wherein The weight percentage of the anti-wrinkle composition in the cosmetic product is 0.5-10%.

10. The cosmetic product according to claim 8, wherein The cosmetic product further comprises at least one cosmetically acceptable adjuvant.

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