Method for preparation of environment friendly disinfectant formulation

Abstract

Disclosed herein is a method for preparation of a disinfectant formulation, the method comprising steps of obtaining a chlorine dioxide (CIO2) solution by adding a predetermined quantity of sodium chlorite with a predetermined quantity of sodium bisulfate in a first container filled with water, keeping the first container closed for a predetermined period of time, dissolving a predetermined quantity of peracetic acid (PAA) in water in a second container, adding a predetermined quantity of benzalkonium chloride (BAC) to the solution in the second container, and mixing and stirring the solutions of the first and second container to obtain a disinfectant solution.

Description

[0001] METHOD FOR PREPARATION OF ENVIRONMENT FRIENDLY

[0002] DISINFECTANT FORMULATION

[0003] TECHNICAL FIELD

[0004]

[0001] The present invention pertains broadly to the chemical and disinfectant industry. More particularly, it relates to a disinfectant composition that has remarkable sterilization and disinfection power and method of manufacturing the disinfectant.

[0005] BACKGROUND OF THE INVENTION

[0006]

[0002] Disinfection plays a critical role in preventing the spread of infectious diseases caused by bacteria, fungi, viruses, and other pathogens. Each disinfectant has unique characteristics, such as its ability to target specific types of microorganisms, its concentration requirements, its compatibility with different surfaces or materials, and its safety considerations. Therefore, disinfectants are not interchangeable, and incorrect concentrations and inappropriate disinfectants can result in excessive costs. Additionally, selection of disinfectants should be based on various factors such as targeted microorganisms, availability of disinfectants, etc. Moreover, traditional disinfectants, while effective, often come with drawbacks such as toxicity, corrosiveness, and environmental impact.

[0007]

[0003] There are various approaches and products that have been used previously for disinfection. Chlorine-based disinfectants have long been a staple, offering broad- spectrum efficacy but also posing risks due to their corrosive nature. Peracetic acid-based disinfectants have shown rapid action against microorganisms, yet their concentrated forms can be corrosive and irritating. Alcohol-based disinfectants provide effectiveness when appropriately diluted, but their flammability and quick evaporation present challenges. Hydrogen peroxide-based disinfectants offer broad-spectrum action but require careful handling due to instability. Aldehydes and phenolic-based disinfectants have their strengths but also exhibit drawbacks such as health concerns and environmental impact.

[0008]

[0004] Despite their effectiveness, traditional disinfectants come with significant shortcomings. Many of these products can cause skin and eye irritation, respiratory issues, and contribute to environmental pollution if not handled and disposed of properly. The corrosiveness and toxicity of certain disinfectants pose risks to both human health and the surfaces they are applied to, leading to potential damage over time. Additionally, the quick evaporation of some disinfectants limits their efficacy, requiring frequent reapplication for sustained effectiveness. These drawbacks highlight the need for innovative solutions that can achieve efficient disinfection without compromising safety or environmental sustainability.

[0009]

[0005] Therefore, there is an evident need to provide a new approach to achieving effective disinfectants while considering environmental impact. The synergistic combination of active ingredients contributes to the formulation's broad spectrum of activity and rapid action. In addition, choosing less toxic, non-corrosive and environmentally friendly alternatives whenever possible can help minimize risks to human health and the environment.

[0010] SUMMARY OF THE INVENTION

[0011]

[0006] Embodiments of the present disclosure present technological improvements as solutions to one or more of the above-mentioned technical problems recognized by the inventor in conventional solutions.

[0012]

[0007] According to a first aspect of the present invention there is provided a method for preparation of a disinfectant formulation, the method comprising steps of: obtaining a chlorine dioxide (CIO2) solution by adding a predetermined quantity of sodium chlorite with a predetermined quantity of sodium bisulfate in a first container filled with water; keeping the first container closed for a predetermined period of time; dissolving a predetermined quantity of peracetic acid in water in a second container; adding with a predetermined quantity of benzalkonium chloride to the solution in the second container; and mixing and stirring the solutions obtained of the first and second container to obtain a disinfectant solution.

[0013]

[0008] In accordance with an embodiment of the present invention, chlorine dioxide solution (CIO2) is in the range of 750 - 1500 ppm, peracetic acid (PAA) in the range of 0.1 - 0.2 % and adding the benzalkonium chloride (BAC) in the range of 0.15 - 0.2 %.

[0014]

[0009] In accordance with an embodiment of the present invention, the predetermined quantity of sodium chlorite is in the range of 3-3.6 grams, the predetermined quantity of sodium bisulfate is in the range of 3-3.8 grams, the predetermined quantity of water is in the range of 490-510 grams.

[0015]

[0010] In accordance with an embodiment of the present invention, the first container is kept closed for a predetermined period of time in the range of 2-3 hours.

[0016] [Oil] In accordance with an embodiment of the present invention, the second solution is prepared by a predetermined quantity of water in the range of 480-490 grams.

[0017]

[0012] In accordance with an embodiment of the present invention, stirring of the first, second and third solution is done for a time period of 3-7 minutes.

[0013] In accordance with an embodiment of the present invention, a composition A is a solution of Chlorine Dioxide at a concentration of 1500 ppm and Benzalkonium Chloride at a concentration of 0.15% and Peracetic Acid at a concentration of 0.2%.

[0018]

[0014] In accordance with an embodiment of the present invention, a composition B is a solution of Chlorine Dioxide at a concentration of 1000 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.15%.

[0019]

[0015] In accordance with an embodiment of the present invention, a composition C is a solution of Chlorine Dioxide at a concentration of 1500 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.15%.

[0020]

[0016] In accordance with an embodiment of the present invention, a composition D is a solution of Chlorine Dioxide at a concentration of 750 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.1%.

[0021]

[0017] According to a second aspect of the present invention, refers to a disinfectant formulation comprising a predetermined quantity of chlorine dioxide (CIO2); a predetermined quantity of benzalkonium chloride (B AC) and a predetermined quantity of peracetic acid (PAA).

[0022]

[0018] In accordance with an embodiment of the present invention, the predetermined concentration of chlorine dioxide (CIO2) is in the range of 750 - 1500 ppm.

[0023]

[0019] In accordance with an embodiment of the present invention, the predetermined quantity of benzalkonium chloride (BAC) is in the range of 0.15 - 0.2 %.

[0024]

[0020] In accordance with an embodiment of the present invention, the predetermined quantity of peracetic acid (PAA) in the range of 0.1 - 0.2 %.

[0025] BRIEF DESCRIPTION OF DRAWINGS

[0026]

[0021] So that the manner in which the above recited features of the present invention can be understood in detail, a more particular description of the invention, briefly summarized above, may have been referred by embodiments, some of which are illustrated in the appended drawings. It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered limiting of its scope, for the invention may admit to other equally effective embodiments.

[0027]

[0022] These and other features, benefits, and advantages of the present invention will become apparent by reference to the following text figure, with like reference numbers referring to like structures across the views, wherein:

[0028] FIG. 1 illustrates an exemplary method diagram for preparation of a disinfectant formulation. DETAILED DESCRIPTION OF EMBODIMENTS

[0029]

[0023] The present invention is described hereinafter by various embodiments with reference to the accompanying drawing, wherein reference numerals used in the accompanying drawing correspond to the like elements throughout the description.

[0030]

[0024] While the present invention is described herein by way of example using embodiments and illustrative drawings, those skilled in the art will recognize that the invention is not limited to the embodiments of drawing or drawings described and are not intended to represent the scale of the various components. Further, some components that may form a part of the invention may not be illustrated in certain figures, for ease of illustration, and such omissions do not limit the embodiments outlined in any way. It should be understood that the drawings and detailed description thereto are not intended to limit the invention to the particular form disclosed, but on the contrary, the invention is to cover all modifications, equivalents, and alternatives falling within the scope of the present invention as defined by the appended claim. As used throughout this description, the word "may" is used in a permissive sense (i.e., meaning having the potential to), rather than the mandatory sense, (i.e., meaning must). Further, the words "a" or "an" mean "at least one” and the word “plurality” means “one or more” unless otherwise mentioned. Furthermore, the terminology and phraseology used herein is solely used for descriptive purposes and should not be construed as limiting in scope. Language such as "including," "comprising," "having," "containing," or "involving," and variations thereof, is intended to be broad and encompass the subject matter listed thereafter, equivalents, and additional subject matter not recited, and is not intended to exclude other additives, components, integers, or steps. Likewise, the term "comprising" is considered synonymous with the terms "including" or "containing" for applicable legal purposes.

[0031]

[0025] Further, the various embodiments described herein below include specific method steps in an exemplary order but a wide variety of other such method steps could be implemented within the scope of the invention, including additional steps, omission of some steps, or performing the method in a different order.

[0032]

[0026] According to one embodiment of the present invention, disinfectants may be classified as active compounds used to eradicate microbes / pathogens such as bacteria, fungi, and viruses, among others. Also known as microorganisms, they are too small to perceive with the human eye and can be found on surfaces such as the floor, water, and clothes. A disinfectant is used for commercial, industrial, medicinal, and household disinfecting purposes. An embodiment of the present invention describes a disinfectant formulation for sterilization and disinfection. The disinfection formulation may include, but not limited to, killing broad-spectrum microbes / pathogens, including yeast and mould Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Escherichia coli, and Salmonella.

[0033]

[0027] The present invention aims to prevent the spreading of infectious diseases caused by bacteria, fungi, viruses, and other pathogens. It offers an effective solution of sterilization by proposing a method for preparation of a disinfectant formulation by using chlorine dioxide, Peracetic acid and benzalkonium chloride. The invention introduces a groundbreaking disinfectant composition and manufacturing method that revolutionize the field of pathogen control. The composition, meticulously crafted for exceptional sterilization and disinfection power, comprises a unique blend of active ingredients known for their potency against a broad spectrum of pathogens. Notably, this formulation is designed with environmental friendliness in mind, aligning with modem sustainability standards. This composition is capable of sterilizing medical, laboratory equipment, surfaces, rooms and tools. The method further aims to prepare chlorine dioxide by reaction of Sodium Chlorite with Sodium Bisulfate and streamline the manufacturing process that ensures consistent and reproducible results, optimizing the composition's efficacy and reducing manufacturing costs unlike traditional approaches that rely on high-cost generators to prepare chlorine dioxide gas and then dissolving it with a water to obtain a solution of Chlorine Dioxide.

[0034]

[0028] The present invention is now described in detail with reference to accompanying drawing.

[0035]

[0029] FIG. 1 illustrates a method 100 for preparation of a disinfectant formulation, in accordance with an embodiment of the present invention.

[0036]

[0030] The method 100, comprises a unique blend of environment friendly active ingredients such as Chlorine dioxide solution (CIO2), Benzalkonium chloride (BAC), and Peracetic acid (PAA) at defined concentrations.

[0037]

[0031] The method 100 clearly starts at step 102, obtaining a chlorine dioxide (CIO2) solution by adding a predetermined quantity of sodium chlorite in the range of 3-3.6 grams with a predetermined quantity of sodium bisulfate in the range of 3-3.8 grams in a first container filled with a predetermined quantity water in the range of 490-510 grams. The chlorine dioxide solution (CIO2) is prepared in the range of 750 - 1500 ppm. Chlorine dioxide may also be produced by reaction of sulfuric acid with potassium chlorate. In modern times, sodium chlorate is used instead. Its applications are diverse, including but not limited to, cleaning circuit boards, treating sulfides in the oil industry, bleaching textiles and candles, and even as a wartime substitute for scarce chlorine bleach. Notably, it's favoured over chlorine for paper bleaching due to producing clearer and stronger fibres and generating fewer harmful byproducts. Chlorine dioxide is extensively used for sterilization in medical and laboratory settings, effectively killing various pathogens and preventing biofilm formation. Its efficacy extends to disinfecting surfaces, rooms, and tools, and it is potent against anthrax spores.

[0038]

[0032] Chlorine dioxide has various characteristics including, but not limited to, being an excellent bacterial disinfectant, and it is even more effective than chlorine in disinfecting virus - contaminated water. Chlorine Dioxide has regained popularity because it successfully deactivates the chlorine-resistant diseases Giardia and Cryptosporidium. Disinfecting with CIO2 does not produce unpleasant odours rather, it eliminates phenols, which can create odour and taste problems. It does not react with ammonia nitrogen, amines, or other oxidizable organic compounds. It also eliminates chemicals that can produce trihalomethanes and enhances coagulation. CIO2 is more effective than chlorine at removing iron and manganese, particularly when they are present in complex compounds.

[0039]

[0033] Chlorine Dioxide can be used as oxidizer or disinfectant. It is a very strong oxidizer, and it effectively kills pathogenic microorganisms such as fungi, bacteria, and viruses. It also prevents and removes bio films. Chlorine Dioxide has many applications, it is used in the electronics industry to clean circuit boards, in the oil industry to treat sulfides and to bleach textile and candles.

[0040]

[0034] Then at step 104, keeping the first container closed for a predetermined time period of 2-3 hours. Closing the container creates a controlled environment that allows the chemical reaction between sodium chlorite and sodium bisulfate to proceed undisturbed. The reaction takes time to reach completion, and maintaining a closed system ensures that the reaction continues until the desired amount of chlorine dioxide is produced. Further, the contents of the first container are not mixed immediately after adding the chemicals for the reaction to proceed homogeneously and efficiently. Mixing the contents too early could disrupt the initial stages of the reaction or lead to uneven distribution of reactants, affecting the overall efficacy of chlorine dioxide production. The reaction between sodium chlorite and sodium bisulfate may release gases or heat, which could be hazardous if not properly controlled. Keeping the container closed helps contain any byproducts or reaction-related effects, contributing to a safer manufacturing process.

[0041]

[0035] Chlorine Dioxide (CIO2) is a powerful disinfectant and antimicrobial agent that is effective against a wide range of microorganisms, including bacteria, germs (viruses), and fungi. Its mode of action and effectiveness makes it a valuable choice for various disinfection and water treatment applications. Chlorine Dioxide is highly effective against bacteria, including both Grampositive and Gram-negative bacteria. It works by disrupting bacterial cell membranes and interfering with cellular processes, leading to cell death. It is effective against a wide range of viruses, including but not limited to enveloped and non-enveloped viruses. It can damage the protein coat of viruses and disrupt their genetic material, rendering them inactive. Chlorine Dioxide is further effective against fungi and yeast species. It disrupts fungal cell membranes and enzymes, leading to cell death.

[0042]

[0036] At step 106, dissolving a predetermined quantity of peracetic acid in predetermined quantity of water in the range of 480-490 grams in a second container. Peracetic acid is frequently used in the food industry for disinfecting surfaces and equipment, as well as sanitizing during processing. Peracetic Acid is used in healthcare settings to sterilize medical equipment and instruments. Peracetic Acid (PAA) is a powerful disinfectant and antibacterial agent that kills bacteria, viruses, germs and fungi. This disinfectant is widely used in numerous industries due to its broad- spectrum antibacterial activity, fast action, and ability to break down into harmless byproducts. Its efficiency and manner of action make it suitable for disinfection and sterilizing applications.

[0043]

[0037] In another embodiment of present disclosure, Peracetic Acid (PAA) restricts the growth of microorganisms such as, but not limited to, bacteria, germs (viruses) and fungi. Peracetic Acid is highly effective against bacteria, including both Gram-positive and Gram-negative bacteria. It works by disrupting the cell membranes and cellular proteins of bacteria, leading to cell death. Peracetic Acid is effective against a wide range of viruses, including enveloped and non-enveloped viruses. It can damage the protein coat of viruses and disrupt their genetic material, rendering them inactive. Peracetic Acid is effective against fungi and yeast species. PAA disrupts fungal cell membranes and enzymes, leading to cell death. It is also used in healthcare facilities and laboratories for the disinfection of surfaces and medical equipment to prevent the spread of viral infections. It can be used in agriculture, food production, and pharmaceutical manufacturing to control fungal contamination.

[0044]

[0038] Then, step 108, by adding a predetermined quantity of benzalkonium chloride to the solution in the second container. Benzalkonium Chloride is widely used for surface disinfection in industries such as food processing, healthcare, and pharmaceutical manufacturing. Its effectiveness against microbes such as, but not limited to, bacteria, germs, and fungi can vary depending on several factors, including the concentration of BAC in the solution, contact time, and the specific microorganisms in question. BAC is a staple ingredient in various pharmaceutical products such as, but not limited to, hand sanitizers, wound disinfectants, nasal sprays, and eye drops. Its versatility extends to surface disinfection across industries such as, but not limited to, food processing, healthcare, and pharmaceutical manufacturing, where it's commonly found in wipes, cleaners, and sanitizers.

[0045]

[0039] Benzalkonium Chloride is effective against a wide range of bacteria, including both Gram-positive and Gram-negative bacteria. It disrupts the cell membranes of bacteria, leading to cell leakage, loss of cellular components, and ultimately bacterial cell death. BAC is generally effective against many fungi and yeast species. Similar to its action against bacteria, BAC disrupts the cell membranes of fungal cells, leading to cell damage and death. Benzalkonium Chloride has limited effectiveness against viruses, particularly enveloped viruses (viruses surrounded by a lipid membrane). It can disrupt the lipid envelope of some enveloped viruses, rendering them inactive. However, BAC may be less effective against non-enveloped viruses, which lack a lipid membrane. BAC is often used in disinfectant sprays, surface wipes, and hand sanitizers to reduce bacterial contamination, in addition to that, it is commonly used in antifungal solutions for topical applications.

[0046]

[0040] In step 110, mixing and stirring the solutions of the first and second container to obtain a disinfectant solution. Stirring of the first, second and third solution is done constantly for a time period of about 3-7 minutes. The composition of the disinfectant has its application in various fields. Natural sources of Chlorine Dioxide, Benzalkonium Chloride, and Peracetic Acid are not typically found in nature in significant quantities or in their pure forms. These chemicals are primarily produced through chemical synthesis or industrial processes. Chlorine Dioxide can be generated naturally in small amounts through processes like the reaction of chlorine with organic matter in water, especially in sunlight. However, this natural formation is minimal and not sufficient for practical disinfection purposes. While Benzalkonium Chloride is primarily synthesized industrially, some microorganisms in nature can produce quaternary ammonium compounds (QACs) similar to BAC. Peracetic Acid can be produced by certain microorganisms as part of their metabolic processes. In natural environments, such as soil and water, microbial activities can lead to the formation of peracetic acid through oxidation reactions involving organic compounds and oxygen.

[0047]

[0041] In an alternate embodiment of the present disclosure, the disinfectant with composition A is prepared with Chlorine Dioxide at a concentration of 1500 ppm and Benzalkonium Chloride at a concentration of 0.15% and Peracetic Acid at a concentration of 0.2%. This composition can be prepared for sterilization and disinfection of swimming pool water, water treatment in bathhouses. For instance, in a swimming pool scenario, this formulation effectively eliminates harmful bacteria, viruses, and fungi, ensuring a safe and hygienic swimming environment for patrons. Similarly, in bathhouses where water quality is crucial for maintaining cleanliness and preventing infections, this composition provides reliable disinfection, safeguarding the health of bathers. In an alternate embodiment, higher concentrations of BAC may be suitable for hospitalgrade disinfectants. For example, in a hospital setting, where the need for stringent disinfection is paramount to prevent healthcare-associated infections, an increased concentration of BAC in the formulation enhances its efficacy against a broader range of pathogens, including antibiotic- resistant strains. This expanded formulation maintains its effectiveness in sterilizing and disinfecting swimming pool water and water treatment in bathhouses while also catering to more demanding environments such as hospitals, where infection control is of utmost importance.

[0048]

[0042] In another alternate embodiment of the present disclosure, the second composition B is a solution of Chlorine Dioxide at a concentration of 1000 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.15%. This composition can be prepared for sterilization and disinfection of general surfaces and public facilities. For instance, in public restrooms or transportation hubs where frequent human contact occurs, this formulation effectively eliminates a wide range of pathogens, including bacteria and viruses, ensuring a clean and hygienic environment for the public. In alternate embodiment, lower concentrations of BAC may be used in consumer products like hand sanitizers. For example, in daily use scenarios such as offices, schools, or homes, hand sanitizers containing this lower concentration of BAC offer convenient and effective hand hygiene solutions. The reduced concentration maintains efficacy against common pathogens while ensuring safety for repeated use on skin, making it ideal for personal hygiene applications.

[0049]

[0043] In yet another alternate embodiment of the present disclosure, the third composition C is solution of Chlorine Dioxide at a concentration of 1500 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.15%. This composition can be prepared for sterilization and disinfection of clothes. For example, in laundry facilities or textile industries, where maintaining cleanliness and eliminating pathogens from fabrics is essential, this formulation offers effective disinfection, ensuring hygienic garments for consumers.

[0050]

[0044] In yet another alternate embodiment of the present disclosure, the fourth composition D is solution of Chlorine Dioxide at a concentration of 750 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.1%. This composition can be prepared for sterilization and disinfection of Cow and poultry sheds. For instance, in agricultural settings where maintaining a healthy environment for livestock is crucial for preventing diseases and ensuring food safety, this formulation provides robust disinfection, eliminating harmful pathogens from animal housing facilities.

[0051]

[0045] The selection of Chlorine Dioxide, Benzalkonium Chloride, and Peracetic Acid for the disinfectant formulation is based on their unique properties that collectively contribute to potent and versatile disinfection capabilities. Firstly, these compounds exhibit broad-spectrum activity against a wide array of microorganisms, encompassing bacteria, viruses, and fungi. This broad effectiveness ensures thorough disinfection across different environments and scenarios. Secondly, each compound operates through distinct mechanisms of action. Chlorine Dioxide acts as a powerful oxidizing agent, disrupting cellular processes in microorganisms. Benzalkonium Chloride, a quaternary ammonium compound, targets cell membranes, leading to microbial destruction. Peracetic Acid, another potent oxidizing agent, inflicts damage on cell structures, further enhancing disinfection efficacy. When combined, these compounds often exhibit synergistic effects, amplifying their overall disinfection prowess. Moreover, they offer versatility in application, suitable for disinfecting water (Chlorine Dioxide), surfaces (Benzalkonium Chloride), and textiles (Peracetic Acid), making the formulation adaptable to diverse settings ranging from healthcare facilities to public spaces. Importantly, these compounds are chosen for their relative environmental friendliness compared to traditional disinfectants, as they break down into harmless byproducts post-use, minimizing ecological impact.

[0052]

[0046] Disinfection effectiveness test has been conducted for disinfectant formulation A, B, C, and D which has been performed against the following types of microorganisms: Enumeration of Yeast & Mould, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Escherichia coli and Salmonella in solid samples such as food, soil, or other solid substrates, represented by CFU / g. The results are shown below in the tables 1, 2, 3 and 4 respectively.

[0053] In addition to disinfection effectiveness, another test has been conducted also for disinfectant formulation A, B, C, and D which has been performed against the following types of microorganisms: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Enterobacter hirae in liquid samples such as water, broth, or liquid cultures, represented by CFU / ml.

[0054] The results are shown below in the tables 5, 6, 7 and 8 respectively.

[0055] Note:

[0056] CFU / g indicates the number of colonies of viable microorganisms capable of forming on a solid growth area per gram of solid sample.

[0057] CFU / ml indicates the number of viable microorganism colonies that are capable of forming on a solid growth area per millilitre of liquid sample.

[0058] Table. 1

[0059] Table. 2

[0060] Table. 3

[0061] Table. 4

[0062] Table. 5

[0063] Table. 6

[0064] Table. 7 \l« StOtiiOi <X,i> Si I M ICAH . ' !

[0065] Table. 8

[0066]

[0047] According to tables 1, 2, 3 and 4 the compositions A, B, C and D show less than 10 cfu / g (colony-forming unit per gram) for test on enumeration of Yeast & Mould Content whereas no colonies are detected for microbe species Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Escherichia coli, Salmonella spp. Thus, the disinfectant exhibited effectiveness against various pathogens, including bacteria, viruses, and fungi species. In controlled experiments, Polysorbate 80 is used as neutralizer to overcome residues from the other compounds to avoid the chances of false negative results. Eugon LT 100 Broth is used as diluted fluid to maintain the culture of bacteria and Sabouraud Dextrose Agar (SDA) is used as media for the enumeration of yeast & mould.

[0067]

[0048] Further, TSA is used for Mesophilic Aerobic Bacterial Count. According to Tables 5, 6, 7, and 8 formulations A, B, C, and D respectively show significant reductions in enumeration (or viability) of Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, and Enterobacter hirae during a contact time of five minutes in both clean, dirty conditions. According to tables 5, 6, 7 and 8 the reduction in viability of Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, and Enterobacter hirae in clean and dirty conditions depicts that the efficacy of the disinfectant is high in dirty conditions too. Reduction in viability or reproducibility of microorganisms in both of these conditions highlights the efficacy of the disinfectant to control the growth of microbes. For example, Formulation A (table 5) shows a reduction in viability of Pseudomonas aeruginosa during five minutes from log 7.29 to log 2.04 in clean conditions and from log 7.29 to log 2.16 in dirty conditions demonstrates that the disinfectant is achieved a log 5.25 reduction and in a clean conditions and achieved a log 5.13 reduction and in dirty conditions during a five-minute contact time, significantly lowering the number of bacterial colonies, Although slightly less than in clean conditions, this still indicates a very high level of effectiveness. this means that the disinfectant reduced the bacterial count by 99.999% which indicates a strong disinfecting power in both of the two conditions. In table 6, formulation B is effective in reduction of viability of the above- mentioned organisms, for example, the viability of Pseudomonas aeruginosa is reduced in clean conditions from log 7.29 to log 1.88 and from log 7.29 to log 2.13 in dirty conditions during 5 minutes. According to table 7, for example again in case of Pseudomonas aeruginosa, formulation C of the disinfectant is capable of reduction of viability of the microorganisms from log 7.29 to log 2.02 in clean conditions and from log 7.29 to log 2.26 in dirty conditions during 5 minutes. According to table 8 of formulation D, yet again in case of Pseudomonas aeruginosa, the reduction in viability of organisms is seen from log 7.29 to log 1.95 in clean conditions and from log 7.29 to log 2.08 in dirty conditions during 5 minutes.

[0068] Thus, the disinfectant has shown its effectiveness against various pathogens, including bacteria, viruses and fungal species with a microbial reduction rate of 99.999% within a contact time of 5 minutes.

[0069]

[0049] This invention, with its remarkable sterilization and disinfection power, can be applied across various domains. Here are some possible use-cases:

[0070] • Disinfection of public places: The composition can be used at different concentrations of the three compounds namely Chlorine Dioxide, Benzalkonium Chloride and Peracetic Acid for the purpose of cleaning swimming pool water, general surfaces, and other public facilities.

[0071] • Healthcare Facilities: Hospitals, clinics, and medical laboratories can use the present disinfectant composition to sanitize surfaces, medical equipment, and healthcare environments. It can help prevent healthcare-associated infections (HAIs) and ensure a safe and sterile environment for patients and healthcare workers.

[0072] • Water Treatment and Purification: Water treatment facilities, including municipal water treatment plants and private water purification systems, can benefit from this disinfectant composition for treating water supplies. It effectively eliminates bacteria, viruses, and other pathogens, ensuring safe and potable water for consumption.

[0073] • Veterinary and Animal Care Facilities: Veterinary clinics, animal shelters, and animal farms can use this disinfectant to sanitize animal enclosures, equipment, and surgical instruments. It helps control the spread of infectious diseases among animals and maintains hygienic conditions in animal care settings. It is also used in farms for disinfection of Cow and poultry sheds. • Domestic use: Homeowners can use this disinfectant composition for household cleaning, sanitizing surfaces, and maintaining a hygienic living environment. It is suitable for disinfecting kitchens, bathrooms, living areas, pools and commonly touched surfaces.

[0074] • Pharmaceutical industry: The method 100 for preparation of a disinfectant formulation is used to control fungal contamination. The composition can be used to disinfect pharmaceutical manufacturing facilities, laboratories, and medical equipment to prevent microbial contamination and ensure product safety.

[0075] • Food Processing Industry: Food processing plants, including those handling fruits, vegetables, meats, and dairy products, can utilize the method 100 for preparation of disinfectant to sanitize production equipment, processing surfaces, and packaging materials.

[0076] • Agriculture applications: Agricultural equipment, such as machinery used in farming operations or food processing facilities, can be disinfected using this composition to prevent the spread of pathogens. It may also be utilized for crop protection by disinfecting storage areas, irrigation systems, and handling equipment.

[0077]

[0050] Each of these use-cases involves the deployment of the method 100 for preparation of a disinfectant formulation using chlorine dioxide, peracetic acid and benzalkonium chloride.

[0078]

[0051] The invention presents several advantages that can significantly enhance pathogen eradication properties of a disinfectant:

[0079] 1. Superior Efficacy: The composition exhibits remarkable sterilization and disinfection power, effectively reducing microbial populations by over 99.9% within a short contact time.

[0080] 2. Effective against Broad Spectrum Microbes: It is effective against a wide range of pathogens, including bacteria, viruses, germs, and fungi, making it versatile for various disinfection applications.

[0081] 3. Environmentally Friendly: The composition comprises environmentally friendly active ingredients, reducing the impact on the environment compared to traditional disinfectants.

[0082] 4. Cost Effective: Unlike traditional methods that rely on high-cost generators for chlorine dioxide, the manufacturing method in this invention is cost-effective and efficient.

[0083] 5. Odor Control: The composition removes and prevents biofilm, destroys phenols that can cause odor and taste problems, and does not cause odor nuisance during disinfection.

[0084] 6. Reduce Health risks: By effectively controlling microbial contamination, the invention reduces health risks associated with pathogens in water, surfaces, and agricultural settings, while also minimizing the formation of harmful disinfection byproducts.

[0085]

[0052] These advantages collectively contribute to enhancing the efficacy against broad spectrum of pathogens and building an environmentally friendly disinfectant.

[0053] Various modifications to these embodiments are apparent to those skilled in the art from the description and the accompanying drawings. The principles associated with the various embodiments described herein may be applied to other embodiments. Therefore, the description is not intended to be limited to the embodiments shown along with the accompanying drawings but is to be providing broadest scope of consistent with the principles and the novel and inventive features disclosed or suggested herein. Accordingly, the invention is anticipated to hold on to all other such alternatives, modifications, and variations that fall within the scope of the present invention and the appended claims.

Claims

CLAIMS1. A method for preparation of a disinfectant formulation, the method comprising steps of: obtaining a chlorine dioxide (CIO2) solution by adding a predetermined quantity of sodium chlorite with range of 3-3.6 grams with a predetermined quantity of sodium bisulphate with range of 3-3.6 grams in a first container filled with water in the range of 490-510 grams; keeping the first container closed for a predetermined period. Dissolving a predetermined quantity of peracetic acid (PAA) in water with range of 480-490 grams in a second container, adding a predetermined quantity of benzalkonium chloride (BAC) to the solution in the second container; and mixing and stirring the solutions of the first and second container to obtain a disinfectant solution.

2. The method as claimed in claim 1, wherein chlorine dioxide solution (CIO2) is in the range of 750 - 1500 ppm, peracetic acid (PAA) in the range of 0.1 - 0.2 % and adding the benzalkonium chloride (BAC) in the range of 0.15 - 0.2 %.

3. The method as claimed in claim 1, wherein the first container is kept closed for a predetermined period of time in the range of 2-3 hours.

4. The method as claimed in claim 1, wherein, stirring of the first, second and third solution is done for a time period of 3-7 minutes.

5. The method as claimed in claim 1, wherein a composition A is a solution of Chlorine Dioxide at a concentration of 1500 ppm and Benzalkonium Chloride at a concentration of 0.15% and Peracetic Acid at a concentration of 0.2%.

6. The method as claimed in claim 1, wherein a composition B is a solution of Chlorine Dioxide at a concentration of 1000 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.15%.

7. The method as claimed in claim 1, wherein a composition C is a solution of Chlorine Dioxide at a concentration of 1500 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.15%.

8. The method as claimed in claim 1, wherein a composition D is a solution of Chlorine Dioxide at a concentration of 750 ppm and Benzalkonium Chloride at a concentration of 0.2% and Peracetic Acid at a concentration of 0.1%.

9. A disinfectant formulation, the disinfectant formulation comprising: a predetermined concentration of chlorine dioxide (CIO2); a predetermined quantity of benzalkonium chloride (BAC); and a predetermined quantity of peracetic acid (PAA).

10. The disinfectant formulation as claimed in claim 1, wherein the predetermined concentration of chlorine dioxide (CIO2) is in the range of 750 - 1500 ppm.

11. The disinfectant formulation as claimed in claim 1, wherein the predetermined quantity of benzalkonium chloride (BAC) is in the range of 0.15 - 0.2 %.

12. The disinfectant formulation as claimed in claim 1, wherein the predetermined quantity of peracetic acid (PAA) in the range of 0.1 - 0.2 %.

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