POCT hematology analyzer and hematology analysis method

Abstract

The present application discloses a POCT hematology analyzer and a hematology analysis method. In the POCT hematology analyzer, a pressure buildup mechanism performs pressure detection on a test chamber during testing of a reagent cartridge; a processor determines, on the basis of the pressure detection result from the pressure buildup mechanism, whether the gas tightness of the test chamber is abnormal. Thus, the reagent cartridge can be processed in a timely manner when the gas tightness of the test chamber is abnormal, thereby reducing damage to the POCT hematology analyzer caused by problems such as gas leakage or port blockage of the reagent cartridge, and improving the test accuracy and reliability of the POCT hematology analyzer.

Description

POCT blood cell analyzer and blood cell analysis method TECHNICAL FIELD

[0001] The present application relates to the technical field of blood analysis, in particular to a POCT blood cell analyzer and a blood cell analysis method. BACKGROUND

[0002] At present, the POCT blood cell analyzer usually detects blood cells through a kit. The kit is necessary consumables for the POCT blood cell analyzer, and its production quality will have a certain influence on the accuracy of blood cell analysis results and the detection process.

[0003] During the detection process of the POCT blood cell analyzer, a pressure building mechanism is needed to provide a preset pressure for a detection pool, so that the sample in the detection pool can be tested under the preset pressure. When the air tightness of the detection pool of the kit is abnormal, for example, the detection pool leaks or is blocked, it will cause the accuracy of the sample detection result to decrease during the impedance detection process, or cause the detection waste liquid of the kit to leak, so that the detection waste liquid enters the instrument and causes damage, reducing the accuracy and reliability of the POCT blood cell analyzer. SUMMARY

[0004] The technical problem solved by the present application is how to improve the accuracy and reliability of the POCT blood cell analyzer.

[0005] To solve the above technical problem, the present application provides a first technical solution: a POCT blood cell analyzer is provided, which comprises a bearing mechanism, a pressure building mechanism, a detection mechanism and a processor. The bearing mechanism is used to load a kit, and the detection pool of the kit stores a sample to be detected; the pressure building mechanism is connected with the kit on the bearing mechanism, and is used to provide a preset pressure for the detection pool; the detection mechanism is used to detect the sample in the detection pool under the preset pressure; the processor is connected with the pressure building mechanism and the detection mechanism respectively; the pressure building mechanism is further used to detect the pressure of the detection pool during the detection process of the detection mechanism, and the processor is used to judge whether the air tightness of the detection pool is abnormal according to the pressure detection result of the pressure building mechanism.

[0006] The detection mechanism is used to detect the sample in the detection pool under the preset pressure within a first detection time, the pressure building mechanism detects the pressure of the detection pool for a second detection time, the second detection time is within the first detection time, and the end time of the second detection time is before the end time of the first detection time.

[0007] The pressure building mechanism is configured to perform pressure detection on the detection pool during the detection process to obtain a real-time pressure value of the detection pool, and the processor is configured to determine whether the air tightness of the detection pool is abnormal based on a comparison result of the real-time pressure value and a first set threshold.

[0008] The processor is configured to generate a first warning information when the real-time pressure value is greater than the first set threshold, so as to prompt the kit to leak air through the first warning information.

[0009] The pressure building mechanism is configured to perform pressure detection on the detection pool at intervals of a preset time during the detection process to obtain a pressure change value of the detection pool, and the processor is configured to determine whether the air tightness of the detection pool is abnormal based on a comparison result of the pressure change value and a first threshold range.

[0010] The first threshold range includes a second set threshold and a third set threshold, and the third set threshold is greater than the second set threshold; the processor is configured to generate a first warning information when the pressure change value is greater than the third set threshold, so as to prompt the kit to leak air through the first warning information, and / or the processor is configured to generate a second warning information when the pressure change value is less than the second set threshold, so as to prompt the kit to be blocked through the second warning information.

[0011] The pressure building mechanism is configured to perform pressure detection on the detection pool to obtain a first pressure value during the detection process, and the pressure building mechanism is further configured to continue to perform pressure detection on the detection pool after a preset time interval to obtain a second pressure value, and the processor is configured to calculate a difference between the second pressure value and the first pressure value to obtain the pressure change value.

[0012] The pressure change value is a difference between a pressure value of the detection pool during the detection process and an initial pressure value of the detection pool at the beginning of the test.

[0013] The pressure building mechanism is configured to perform pressure detection on the detection pool to obtain a pressure value of the detection pool within a preset time period during the detection process, and the processor is configured to determine whether the air tightness of the detection pool is abnormal based on a comparison result of a change rate of the pressure value within the preset time period and a second threshold range.

[0014] The second threshold range includes a fourth set threshold and a fifth set threshold, the fifth set threshold is greater than the fourth set threshold; the processor is configured to generate a first warning information when the change rate is greater than the fifth set threshold, so as to prompt the reagent kit to leak through the first warning information, and / or the processor is configured to generate a second warning information when the change rate is less than the fourth set threshold, so as to prompt the reagent kit to be blocked through the second warning information.

[0015] The pressure building mechanism includes a pressure control component, a pressure sensor, and a connecting head, the connecting head is arranged on the bearing mechanism, the pressure control component is connected with the connecting head and the pressure sensor through pipelines, the connecting head is used for docking with the reagent kit, so that the pressure control component provides a preset pressure for the detection pool, and the pressure sensor is used for pressure detection of the detection pool.

[0016] The pressure control component includes a pressure building piece, a negative pressure piece, a first control piece, and a second control piece, the first end of the first control piece is connected with the connecting head, the second end of the first control piece is connected with the first end of the negative pressure piece, the first end of the second control piece is connected with the second end of the negative pressure piece, and the second end of the second control piece is connected with the pressure building piece, the pressure building piece is used for establishing negative pressure for the negative pressure piece, so as to provide the preset pressure for the detection pool when the first control piece is turned on.

[0017] The processor is configured to control the pressure building mechanism to be disconnected with the reagent kit when it is determined that the air tightness of the detection pool is abnormal.

[0018] The detection pool includes a front pool, a rear pool, and a microporous sheet, the front pool is communicated with the rear pool through the microporous sheet, and the detection mechanism is used for counting test on cells in a sample when the sample flows from the front pool to the rear pool through the microporous sheet; the processor is configured to obtain a flow rate of the sample when the sample passes through the microporous sheet, so as to determine whether the air tightness of the detection pool is abnormal according to the flow rate change in the detection process, or the processor is further configured to calculate a flow pressure drop of the sample based on the flow rate, and determine whether the air tightness of the detection pool is abnormal according to the flow pressure drop in the detection process.

[0019] To solve the above technical problems, the second technical solution is provided: a blood cell analysis method is provided, including: receiving a reagent kit, the detection pool of the reagent kit stores a sample to be detected; performing impedance test on the sample of the reagent kit; performing pressure detection on the detection pool in the detection process of the detection mechanism, so as to determine whether the air tightness of the detection pool is abnormal according to the pressure detection result of the pressure building mechanism.

[0020] The beneficial effects of the present application are that, different from the prior art, the POCT blood cell analyzer provided by the present application detects the pressure of the detection pool during the detection process of the kit through the pressure building mechanism, and the processor judges whether the air tightness of the detection pool is abnormal according to the pressure detection result of the pressure building mechanism, so that the kit can be processed in time when the air tightness of the detection pool is abnormal, reducing the damage of the kit to the POCT blood cell analyzer due to air leakage or hole blockage and the like, and improving the detection accuracy and reliability of the POCT blood cell analyzer. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0022] Fig. 1 is a structural schematic diagram of an embodiment of the POCT blood cell analyzer provided by the present application;

[0023] Fig. 2 is an operation schematic diagram of an embodiment of the pressure change curve of the normal detection process provided by the present application;

[0024] Fig. 3 is an operation schematic diagram of an embodiment of the pressure change curve when air leakage occurs provided by the present application;

[0025] Fig. 4 is an operation schematic diagram of an embodiment of the pressure change curve when hole blockage occurs provided by the present application;

[0026] Fig. 5 is a structural schematic diagram of the pressure building mechanism in Fig. 1;

[0027] Fig. 6 is a flow schematic diagram of an embodiment of the blood cell analysis method provided by the present application. Embodiments of the present application

[0028] The present application will be described in further detail below in conjunction with the drawings and embodiments. It is particularly pointed out that the following embodiments are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following embodiments are only some embodiments of the present application, not all embodiments, and all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0029] Reference herein to an "embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are they necessarily mutually exclusive of one another. As will be apparent to those of ordinary skill in the art, embodiments described herein can be combinable with other embodiments.

[0030] In the description of the present application, it is to be noted that unless otherwise explicitly specified and limited, the terms "mounting", "setting", "connecting", "connecting" should be understood in a broad sense, for example, it can be fixedly connected, it can be detachably connected, or integrally connected; it can be mechanically connected, or electrically connected; it can be directly connected, or connected through an intermediate medium. For those skilled in the art, the above-mentioned specific meanings in the present application can be connected according to the specific circumstances.

[0031] Please refer to FIG. 1, which is a structural schematic diagram of an embodiment of a POCT blood cell analyzer provided by the present application. As shown in FIG. 1, the POCT blood cell analyzer of the embodiment of the present application comprises a bearing mechanism 11, a pressure building mechanism 12, a detection mechanism 13, and a processor 14.

[0032] The bearing mechanism 11 is used for loading a reagent box 110, and the detection pool of the reagent box 110 stores a sample to be detected; the pressure building mechanism 12 is connected with the reagent box 110 on the bearing mechanism 11, and the pressure building mechanism 12 is used for providing a preset pressure for the detection pool; the detection mechanism 13 is used for detecting the sample in the detection pool under the preset pressure; the processor 14 is connected with the pressure building mechanism 12, the bearing mechanism 11, and the detection mechanism 13 respectively; the pressure building mechanism 12 is further used for pressure detection of the detection pool during the detection process of the detection mechanism 13, and the processor 14 is used for judging whether the air tightness of the detection pool is abnormal according to the pressure detection result of the pressure building mechanism 12.

[0033] Specifically, the reagent box 110 can comprise a detection pool, the detection pool is used for storing a sample to be detected, and the detection pool can serve as a place for sample detection, so that the detection mechanism 13 performs at least one of impedance detection and optical detection on the sample in the detection pool. In a possible implementation, the detection mechanism 13 can comprise a first detection assembly and a second detection assembly, the first detection assembly is used for performing impedance detection on the sample in the detection pool, and the second detection assembly is used for performing optical detection on the sample in the detection pool. The detection pool comprises a front pool, a rear pool, and a microporous sheet, the microporous sheet is provided with a micropore, the front pool is in communication with the rear pool through the micropore on the microporous sheet, and the sample to be detected is stored in the front pool; when the bearing mechanism 11 loads the reagent box 110, the reagent box 110 is electrically connected with the first detection assembly, and the first detection assembly is used for performing impedance counting on cells in the sample when the sample in the front pool flows to the rear pool through the microporous sheet.

[0034] When the carrying mechanism 11 loads the reagent kit 110, the pressure building mechanism 12 is connected with the reagent kit 110 on the carrying mechanism 11, so that the pressure building mechanism 12 can perform a pressure building operation on the detection cell on the reagent kit 110 to provide a preset pressure for the detection cell. The preset pressure can be a preset positive pressure or a preset negative pressure. For example, the pressure building mechanism 12 can be connected with the front cell of the detection cell, and the pressure building mechanism 12 is configured to provide a preset positive pressure for the front cell to make the sample in the front cell flow to the rear cell through the micropores, or the pressure building mechanism 12 can be connected with the rear cell of the detection cell, and the pressure building mechanism 12 is configured to provide a preset negative pressure for the rear cell to make the sample in the front cell flow to the rear cell through the micropores, which is not limited herein.

[0035] When the pressure of the detection cell is the preset pressure, the pressure building mechanism 12 stops the pressure building operation, and the detection mechanism 13 is configured to detect the impedance of the sample in the detection cell under the preset pressure to make the sample in the front cell flow to the rear cell through the micropores, which causes the pressure in the detection cell to change. For example, when the pressure building mechanism 12 is connected with the front cell of the detection cell, the pressure in the front cell gradually decreases from the preset positive pressure due to the increase of the gas volume in the front cell caused by the sample flowing to the rear cell, or when the pressure building mechanism 12 is connected with the rear cell of the detection cell, the pressure in the rear cell gradually increases from the preset negative pressure due to the decrease of the gas volume in the rear cell caused by the sample flowing to the rear cell. That is, during the normal test process, the pressure of the detection cell changes in a similar trend due to the fixed diameter of the micropores of the micropore sheet. Therefore, the POCT blood cell analyzer of the present embodiment can detect the pressure of the detection cell by the pressure building mechanism 12 during the detection process of the detection mechanism 13 to obtain a pressure detection result, and the processor 14 can determine whether the air tightness of the detection cell is abnormal according to the pressure detection of the pressure building mechanism 12.

[0036] The air tightness of the detection cell refers to whether the front cell or the rear cell of the detection cell has a gas leakage phenomenon during the detection process, or the air tightness can also refer to the flow condition of the front cell and the rear cell during the detection process, for example, whether the front cell and the rear cell can normally flow due to the blockage of the micropores. When the air tightness of the detection cell is normal, the sample and the gas in the front cell can only flow to the rear cell through the micropores of the micropore sheet; when the air tightness of the detection cell is abnormal, according to the connection position of the pressure building mechanism 12, it can include the abnormal decrease of the pressure in the front cell caused by the gas leakage of the front cell or the abnormal decrease of the pressure in the front cell caused by the blockage of the micropores, and it can also include the abnormal increase of the pressure in the rear cell caused by the gas leakage of the rear cell or the abnormal increase of the pressure in the rear cell caused by the blockage of the micropores, which is not limited herein.

[0037] In the embodiment, the POCT blood cell analyzer in the embodiment detects the pressure of the detection pool in the detection process of the kit 110 through the pressure building mechanism 12, and the processor 14 judges whether the air tightness of the detection pool is abnormal according to the pressure detection result of the pressure building mechanism 12, so that the kit 110 can be processed in time when the air tightness of the detection pool is abnormal, and the damage of the kit 110 to the POCT blood cell analyzer due to air leakage or hole blockage and the like is reduced, and the detection accuracy and reliability of the POCT blood cell analyzer are improved.

[0038] In an embodiment, the detection mechanism 13 is configured to detect the sample in the detection pool under the preset pressure within a first detection duration, and the pressure building mechanism 12 is configured to detect the pressure of the detection pool within a second detection duration, the second detection duration being within the first detection duration, and the end time of the second detection duration being before the end time of the first detection duration.

[0039] Specifically, when the detection mechanism 13 detects the sample in the detection pool under the preset pressure, the detection mechanism 13 spends a first detection duration in the detection process, and the pressure building mechanism 12 detects the pressure of the detection pool within a second detection duration, the second detection duration being within the first detection duration, and the end time of the second detection duration being before the end time of the first detection duration. That is, the pressure building mechanism 12 can detect the pressure of the detection pool within any time period within the first detection duration of the detection mechanism 13, but the pressure building mechanism 12 cannot detect the pressure of the detection pool within the last detection time of the detection mechanism 13; for example, the first detection duration of the detection mechanism 13 is divided into a first segment detection, a second segment detection and a third segment detection, and the second detection duration of the pressure building mechanism 12 can be completed within the first segment detection or the second segment detection, but the end time of the pressure detection of the pressure building mechanism 12 cannot be the same as the end time of the third segment detection.

[0040] For example, the detection mechanism 13 detects the sample in the detection pool under the preset pressure within 0-14s, and the pressure building mechanism 12 can detect the pressure of the detection pool within 0-10s, so that there is a time difference between the end time of the pressure detection of the pressure building mechanism 12 and the end time of the sample detection of the detection mechanism 13.

[0041] In the embodiment of the present application, by setting the end time of the second detection duration to be before the end time of the first detection duration, when the POCT blood cell analyzer determines that the air tightness of the detection pool is abnormal, the processor 14 can timely process the reagent kit 110 to avoid the sample in the detection pool from entering the connection channel of the pressure building mechanism 12 and the reagent kit 110 in reverse due to the abnormal air tightness of the detection pool, reduce the possibility of sample pollution to the connection channel inside the POCT blood cell analyzer, and improve the reliability of the POCT blood cell analyzer.

[0042] In an embodiment, the pressure building mechanism 12 is configured to perform pressure detection on the detection pool during the detection process to obtain a real-time pressure value of the detection pool, and the processor 14 is configured to determine whether the air tightness of the detection pool is abnormal based on a comparison result of the real-time pressure value and the first set threshold value.

[0043] Specifically, the POCT blood cell analyzer of the embodiment can perform real-time pressure detection on the detection pool by the pressure building mechanism 12 during the detection process of the detection mechanism 13 to obtain a real-time pressure value of the detection pool. The pressure building mechanism 12 can continuously perform pressure detection on the detection pool within the second detection duration to obtain the real-time pressure value. For example, the pressure building mechanism 12 is configured to perform pressure detection on the detection pool at the beginning of the second detection duration to obtain a first real-time pressure value, the processor 14 is configured to compare the first real-time pressure value with the first set threshold value, and determine whether the air tightness of the detection pool is abnormal based on the comparison relationship between the first real-time pressure value and the first set threshold value. If it is abnormal, it is determined that the detection pool leaks, and the processor 14 can control the disconnection of the pressure building mechanism 12 and the reagent kit 110 and control the unloading of the reagent kit 110 by the carrying mechanism 11. If it is determined that the air tightness of the detection pool is normal, the processor 14 continues to control the detection mechanism 13 to detect the sample in the detection pool, and the processor 14 is further configured to control the pressure building mechanism 12 to continue to perform pressure detection on the detection pool after a preset interval to obtain a second real-time pressure value, and the processor 14 is further configured to continue to determine the second real-time pressure value. The above real-time detection step of the pressure building mechanism 12 ends until the air tightness of the detection pool is determined to be abnormal, or until the second detection duration ends.

[0044] It can be understood that the above-mentioned process of the pressure building mechanism 12 detecting the real-time pressure of the detection pool is performed throughout the second detection duration; the processor 14 can determine that the air tightness of the detection pool is abnormal when the real-time pressure value detected at a certain time is greater than the first set threshold value, or the processor 14 can determine that the air tightness of the detection pool is abnormal when the real-time pressure value is greater than the first set threshold value is detected for two or more times in succession, or the processor 14 can determine that the air tightness of the detection pool is abnormal when the average or standard value of the real-time pressure value is greater than the first set threshold value for two or more times in succession, which is not limited here.

[0045] In the embodiment of the present application, the POCT blood cell analyzer detects the pressure of the detection pool in the detection process of the kit 110 through the pressure building mechanism 12 to obtain a real-time pressure value of the detection pool, and the processor 14 is configured to judge whether the air tightness of the detection pool is abnormal based on a comparison result of the real-time pressure value and the first set threshold, so that the kit 110 can be processed in time when the air tightness of the detection pool is abnormal, reducing the damage of the kit 110 to the POCT blood cell analyzer due to air leakage or hole blockage and improving the detection accuracy and reliability of the POCT blood cell analyzer.

[0046] Optionally, the processor 14 is configured to generate first warning information when the real-time pressure value is greater than the first set threshold, so as to prompt the kit 110 to leak air through the first warning information.

[0047] Specifically, after obtaining the real-time pressure value, the processor 14 is configured to compare the size relationship between the real-time pressure value and the first set threshold, so as to generate the first warning information when the real-time pressure value is greater than the first set threshold, and the first warning information is used to prompt the kit 110 to leak air. Wherein, according to the different connection positions of the pressure building mechanism 12 and the detection pool, the kit 110 leaking air can include the front pool leaking air or the rear pool leaking air and the like.

[0048] Please refer to FIG. 2 and FIG. 3, FIG. 2 is an operation schematic diagram of a pressure change curve in a normal detection process according to an embodiment of the present application, and FIG. 3 is an operation schematic diagram of a pressure change curve when air leakage according to an embodiment of the present application. Since the pressure value has a similar change trend in the process that the sample in the front pool flows to the rear pool through the micropore, exemplarily, since the micropore diameter is fixed and the initial preset pressure is fixed during detection, if there is no external force factor affecting (such as air leakage, hole blockage and the like) in the normal detection process, the change curve of the real-time pressure value detected by the pressure building mechanism 12 in the sample detection process is as shown in FIG. 2. When the kit 110 leaks air and the like, the air leakage will make the gas in the fixed space overflow rapidly, resulting in that the change of the real-time pressure value detected by the pressure building mechanism 12 in the sample detection process is more severe, as shown in FIG. 3. It can be understood that, according to the change of the vertical axis scale in FIG. 2 and FIG. 3, the slope k1 of the pressure change curve in the normal detection process in FIG. 2 is less than the slope k2 of the pressure change curve when air leakage in FIG. 3. That is, in the same unit of time, the pressure change of the detection pool when air leakage is greater than that in the normal detection process.

[0049] As shown in FIG. 2 and FIG. 3, the first set threshold value is generally related to the severity of the air leakage and the initial preset pressure. In an embodiment, the first set threshold value fluctuates based on the real-time pressure value of the pressure change curve of the normal detection process at the same detection time according to the degree of air leakage, for example, the first set threshold value can be set to be greater than a certain value of the real-time pressure value at the same detection time; in another embodiment, the change of the real-time pressure value of the kit 110 can be tested, for example, when the pressure building mechanism 12 is connected to the rear cell for pressure building, the pressure change value of the detection cell during the sample detection process is tested multiple times to obtain multiple experimental schemes of FIG. 2, when it is found that the maximum value of the pressure of the detection cell in the normal sample detection process does not exceed-27kPa, the first set threshold value can be set to-27kPa at this time, so that the processor 14 can quickly determine that the kit 110 has air leakage when the real-time pressure value is greater than the first set threshold value, and generate the first warning information. For example, when the real-time pressure value is-26kPa, the real-time pressure value is greater than the first set threshold value, and the processor generates the first warning information.

[0050] In the embodiments of the present application, the real-time pressure value of the detection cell is obtained by continuously detecting the pressure of the detection cell during the sample detection process, and the processor 14 generates the first warning information when the real-time pressure value is greater than the first set threshold value, so that the kit 110 can be processed in time when the air tightness of the detection cell is abnormal, reducing the damage of the kit 110 to the POCT blood cell analyzer due to air leakage or hole blockage, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

[0051] Further, when the pressure building mechanism is connected to the rear cell of the detection cell, the pressure in the rear cell gradually rises from the preset negative pressure due to the sample in the front cell flowing to the rear cell, and the processor is configured to generate the first warning information when the real-time pressure value is greater than the first set threshold value, i.e., the processor determines the air tightness by the above method. When the pressure building mechanism is connected to the front cell of the detection cell, the pressure in the front cell gradually decreases from the preset positive pressure due to the sample in the front cell flowing to the rear cell, and the processor is configured to generate the first warning information when the real-time pressure value is less than the first set threshold value, and the specific steps are similar to the above, which will not be described here.

[0052] In an embodiment, the pressure building mechanism 12 is configured to perform pressure detection on the detection cell at intervals of a preset time during the detection process to obtain a pressure change value of the detection cell, and the processor 14 is configured to determine whether the air tightness of the detection cell is abnormal based on a comparison result of the pressure change value and the first threshold range.

[0053] Specifically, the POCT blood cell analyzer of the embodiment can perform multiple pressure detections on the detection cell by the pressure building mechanism 12 during the detection process of the detection mechanism 13, and the interval between adjacent two pressure detections is preset time, so as to obtain the pressure change value of the detection cell during the adjacent two pressure detections. The processor 14 is configured to determine whether the air tightness of the detection cell is abnormal based on the comparison result of the pressure change value and the first threshold range. The first threshold range is a threshold interval composed of two set thresholds, and the comparison result of the pressure change value and the first threshold range is used to indicate whether the pressure change value is within the first threshold range, or the comparison result of the pressure change value and the first threshold range is used to indicate the size relationship between the pressure change value and the set threshold in the first threshold range.

[0054] Referring to FIG. 4, FIG. 4 is an operation schematic diagram of an embodiment of the pressure change curve when the hole is blocked. As can be understood, as shown in FIG. 2, during the normal detection process, since the diameter of the micropore is fixed, when the sample in the front cell flows to the rear cell through the micropore, the pressure change value of the detection cell is relatively stable. As shown in FIG. 3, when the reagent cartridge 110 leaks, the leakage will cause the air pressure in the cavity of the front cell or the rear cell to change more greatly, resulting in that the pressure change value of the detection cell will be greater than that during the normal detection process. Therefore, the processor 14 can determine whether the reagent cartridge 110 leaks based on the comparison result of the pressure change value and the first threshold range. As shown in FIG. 4, when the reagent cartridge 110 is blocked, the blockage will cause the sample in the front cell to be difficult to flow to the rear cell through the micropore, and the sample flow speed slows down, resulting in that the pressure change value of the detection cell will be smaller than that during the normal detection process. Therefore, the processor 14 can determine whether the reagent cartridge 110 is blocked based on the comparison result of the pressure change value and the first threshold range. As can be understood, from the change of the vertical axis scale in FIGS. 2-4, the slope k1 of the pressure change curve during the normal detection process in FIG. 2 is greater than the slope k3 of the pressure change curve when the hole is blocked in FIG. 4. That is, k2>k1>k3. In the same unit time, the pressure change of the detection cell when the hole is blocked is smaller than that during the normal detection process.

[0055] In the embodiment, the POCT blood cell analyzer performs pressure detection on the detection cell by the pressure building mechanism 12 during the detection process of the reagent cartridge 110, so as to obtain the pressure change value of the detection cell at the interval of the preset time. The processor 14 is configured to determine whether the air tightness of the detection cell is abnormal based on the comparison result of the pressure change value and the first threshold range. Therefore, when the air tightness of the detection cell is abnormal, the reagent cartridge 110 can be processed in time, the damage of the reagent cartridge 110 to the POCT blood cell analyzer due to leakage or blockage and the like can be reduced, and the detection accuracy and reliability of the POCT blood cell analyzer can be improved.

[0056] Further, as shown in FIGS. 2-4, FIGS. 2-4 are only pressure change curves of the kit 110 in a certain state (for example, in a normal detection state, a gas leakage state or a hole blocking state) during the sample detection process. It can be understood that when the air tightness of the kit 110 changes, the change process can occur during the sample detection process, for example, a gas leakage or hole blocking may occur during the sample detection process, that is, the pressure change curve obtained by the pressure detection of the detection pool by the pressure building mechanism 12 can have two or more segments with different slopes. In this case, the pressure change curve of the kit 110 is not specifically limited.

[0057] Optionally, the first threshold range includes a second set threshold and a third set threshold, the third set threshold is greater than the second set threshold, the processor 14 is configured to generate a first warning information when the pressure change value is greater than the third set threshold, so as to prompt the kit 110 to leak by the first warning information, and / or the processor 14 is configured to generate a second warning information when the pressure change value is less than the second set threshold, so as to prompt the kit 110 to block the hole by the second warning information.

[0058] Specifically, the two endpoints of the first threshold range are the second set threshold and the third set threshold, and the first threshold range is a set composed of values between the second set threshold and the third set threshold. After obtaining the pressure change value, the processor 14 is configured to determine whether the pressure change value is within the first threshold range. When the pressure change value is greater than the third set threshold, the pressure change value of the detection pool is larger than that in the normal detection process, and the processor 14 is configured to generate a first warning information to prompt the kit 110 to leak by the first warning information. When the pressure change value is less than the second set threshold, the pressure change value of the detection pool is smaller than that in the normal detection process, and the processor 14 is configured to generate a second warning information to prompt the kit 110 to block the hole by the second warning information.

[0059] The second set threshold and the third set threshold can be related to at least one of the interval preset time, the diameter of the micropore, the size of the initial preset pressure, etc. The second set threshold and the third set threshold can be obtained by multiple experiments or by conversion of the pressure change value in the normal detection process, which is not specifically limited herein.

[0060] In the embodiments of the present application, the processor 14 is configured to determine the size relationship between the pressure change value and the first threshold range, and generate a first warning information when the pressure change value is greater than the third set threshold, and generate a second warning information when the pressure change value is less than the second set threshold, so that the kit 110 can be processed according to different abnormal reasons when the air tightness of the detection pool is abnormal, the damage of the kit 110 to the POCT blood cell analyzer due to gas leakage or hole blocking is reduced, and the detection accuracy and reliability of the POCT blood cell analyzer are improved.

[0061] Optionally, in an embodiment, the pressure building mechanism 12 is configured to perform a pressure detection on the detection chamber to obtain a first pressure value during the detection process, and the pressure building mechanism 12 is further configured to continue to perform a pressure detection on the detection chamber to obtain a second pressure value after a preset time interval, and the processor 14 is configured to calculate a difference between the second pressure value and the first pressure value to obtain a pressure change value.

[0062] Specifically, the pressure change value is a difference between the second pressure value obtained after the preset time interval and the first pressure value. When the second detection duration is between 0-10s, the preset time interval can be between 0.1s-1s. For example, at the initial time (0s) of the second detection duration, the pressure building mechanism 12 is configured to perform a first pressure detection on the detection chamber to obtain a first pressure value, and after a preset time interval of 0.1s-1s, the pressure building mechanism 12 is configured to perform a second pressure detection on the detection chamber to obtain a second pressure value, and the processor 14 is configured to calculate a difference between the second pressure value and the first pressure value to obtain a first pressure change value; the processor 14 is configured to determine whether the air tightness is abnormal based on the first pressure change value, and when the first pressure change value is within a first threshold range, the processor 14 continues to control the pressure building mechanism 12 to perform a third pressure detection on the detection chamber to obtain a third pressure value after a preset time interval of 0.1s-1s, and the processor 14 is configured to calculate a difference between the third pressure value and the second pressure value to obtain a second pressure change value, and the processor 14 continues to determine the second pressure change value until it is determined that the air tightness of the detection chamber is abnormal, or until the second detection duration ends.

[0063] Further, when the pressure building mechanism is connected to the rear chamber of the detection chamber, the processor determines the air tightness by the above-mentioned manner. When the pressure building mechanism is connected to the front chamber of the detection chamber, the pressure in the front chamber will gradually decrease from the preset positive pressure during the detection process, and the pressure change value mentioned above can be an absolute value of the difference between the second pressure value and the first pressure value, which will not be described herein again.

[0064] Optionally, in another embodiment, the pressure change value is a difference between the pressure value of the detection chamber during the detection process and the initial pressure value of the detection chamber at the beginning of the test.

[0065] Specifically, when the detection mechanism 13 starts to detect the sample in the detection pool, the pressure building mechanism 12 detects the pressure of the detection pool to obtain an initial pressure value, and during a second detection duration after the test starts, the pressure building mechanism 12 continues to detect the pressure of the detection pool at least once to obtain a fourth pressure value, and the pressure change value is the difference between the fourth pressure value and the initial pressure value. For example, at 0s of the first detection duration, the pressure building mechanism 12 detects the pressure of the detection pool to obtain the initial pressure value, and during the second detection duration (for example, within 0-10s) after 0s, the pressure building mechanism 12 can detect the pressure of the detection pool at intervals of a preset time, for example, the pressure building mechanism 12 can detect the pressure of the detection pool at intervals of 0.1s-1s to obtain the fourth pressure value, and the processor 14 is configured to calculate the difference between the fourth pressure value and the initial pressure value to obtain the pressure change value after each pressure detection, and determine whether the air tightness of the detection pool is abnormal based on the comparison result of the pressure change value and the first threshold range.

[0066] In an embodiment, the pressure building mechanism 12 is configured to detect the pressure of the detection pool within a preset time period during the detection process to obtain a change rate of the pressure value of the detection pool within the preset time period, and the processor 14 is configured to determine whether the air tightness of the detection pool is abnormal based on the comparison result of the change rate and the second threshold range.

[0067] Specifically, the POCT blood cell analyzer of the embodiment can detect the pressure of the detection pool at least twice within a preset time period during the detection process of the detection mechanism 13 by the pressure building mechanism 12 to obtain a change rate of the pressure value of the detection pool within the preset time period, and the change rate is used to measure the degree of change of the pressure value of the detection pool with respect to time. The preset time period can be a time period within the second detection duration, for example, when the second detection duration is 0-10s, the preset time period can be a time interval with a first time point and a second time point as two endpoints, and the difference between the second time point and the first time point is the preset time (0.1s-1s) described above. The second detection duration can include multiple preset time periods or be composed of multiple preset time periods, and the processor 14 is configured to determine the air tightness based on the change rate of the pressure value within each preset time period until the air tightness is determined to be abnormal or the second detection duration ends.

[0068] Exemplarily, when the second detection duration includes at least two first preset time periods and a second preset time period, the first preset time period can be between 0s and 1s, and the second preset time period can be between 1s and 2s. The pressure building mechanism 12 is configured to perform pressure detection on the detection pool at 0s to obtain a fifth pressure value, and perform pressure detection on the detection pool at 1s to obtain a sixth pressure value. The processor 14 is configured to calculate a difference between the sixth pressure value and the fifth pressure value, and calculate a ratio of the difference to the preset time period to obtain a first change rate. The processor 14 is configured to determine whether the air tightness is abnormal according to a comparison result of the first change rate and a second threshold range. When the air tightness is normal, the pressure building mechanism 12 is further configured to perform pressure detection on the detection pool at 2s to obtain a seventh pressure value. The processor 14 is configured to calculate a difference between the seventh pressure value and the sixth pressure value, and calculate a ratio of the difference to the preset time period to obtain a second change rate. Alternatively, the processor 14 is configured to calculate a difference between the seventh pressure value and the fifth pressure value, and calculate a ratio of the difference to the preset time period to obtain the second change rate. The determination of the air tightness is abnormal or the second detection duration ends.

[0069] In the embodiments of the present application, the POCT blood cell analyzer detects the pressure of the detection pool in the detection process of the kit 110 by the pressure building mechanism 12 to obtain a change rate of the pressure value of the detection pool in a preset time period. The processor 14 is configured to determine whether the air tightness of the detection pool is abnormal based on a comparison result of the change rate and a second threshold range. In this way, when the air tightness of the detection pool is abnormal, the kit 110 can be processed in time, reducing the damage of the kit 110 to the POCT blood cell analyzer due to air leakage or hole blockage, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

[0070] Alternatively, the second threshold range includes a fourth set threshold and a fifth set threshold, and the fifth set threshold is greater than the fourth set threshold. The processor 14 is configured to generate first warning information when the change rate is greater than the fifth set threshold, so as to prompt the kit 110 to leak air through the first warning information. Alternatively, the processor 14 is configured to generate second warning information when the change rate is less than the fourth set threshold, so as to prompt the kit 110 to block the hole through the second warning information.

[0071] Specifically, the two endpoints of the second threshold range are respectively a fourth set threshold and a fifth set threshold, and the second threshold range is a set consisting of values between the fourth set threshold and the fifth set threshold. After obtaining the change rate, the processor 14 is configured to determine whether the change rate is within the second threshold range. As shown in FIGS. 2-4, the change rate can be associated with the slope of the change curve in the figure. When the change rate is greater than the fifth set threshold, the change rate of the detection pool is greater than that in the normal detection process, and the processor 14 is configured to generate first warning information to prompt the reagent kit 110 to leak through the first warning information. When the pressure change value is less than the fourth set threshold, the change rate of the detection pool is smaller than that in the normal detection process, and the processor 14 is configured to generate second warning information to prompt the reagent kit 110 to be blocked through the second warning information.

[0072] The fourth set threshold and the fifth set threshold can be related to at least one of the length of the preset time period of the detection (i.e., the time difference value of the detection), the diameter of the microwell, the size of the initial preset pressure, etc. The fourth set threshold and the fifth set threshold can be obtained through multiple experiments or through relevant conversion of the change rate in the normal detection process, and are not limited herein.

[0073] In the embodiments of the present application, the processor 14 is configured to determine the size relationship between the change rate and the second threshold range, and generate the first warning information when the change rate is greater than the fifth set threshold, and generate the second warning information when the pressure change value is less than the fourth set threshold, so that the reagent kit 110 can be processed for different abnormal reasons when the air tightness of the detection pool is abnormal, reducing the damage of the reagent kit 110 to the POCT blood cell analyzer due to problems such as leakage or blockage, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

[0074] Further, when the pressure building mechanism is connected to the rear pool of the detection pool, the slope k3 of the pressure change curve when the microwell is blocked is less than the pressure change curve k1 in the normal detection process, and k1 is less than the slope k2 of the pressure change curve when the reagent kit 110 leaks. The processor determines the air tightness by the above method; when the pressure building mechanism is connected to the front pool of the detection pool, the absolute value of the slope k3 of the pressure change curve when the microwell is blocked is less than the absolute value of the pressure change curve k1 in the normal detection process, and the absolute value of k1 is less than the absolute value of the slope k2 of the pressure change curve when the reagent kit 110 leaks. Therefore, when the pressure building mechanism is connected to the front pool of the detection pool, the processor is configured to determine the air tightness according to the comparison result of the absolute value of the change rate and the second threshold range, which is not described herein again.

[0075] In an embodiment, please refer to FIG. 5, which is a structural schematic diagram of the pressure building mechanism in FIG. 1. As shown in FIG. 5, the pressure building mechanism 12 comprises a pressure control assembly, a pressure sensor 121 and a connecting head 122, the connecting head 122 is arranged on the carrying mechanism 11, the pressure control assembly is connected with the connecting head 122 and the pressure sensor 121 through pipelines respectively, the connecting head 122 is used for interfacing with the reagent box 110, so that the pressure control assembly provides a preset pressure for the detection pool, and the pressure sensor 121 is used for detecting the pressure of the detection pool.

[0076] Specifically, the pressure sensor 121 is connected with the pressure control assembly; when the connecting head 122 interfaces with the reagent box 110 and the pipeline between the connecting head 122 and the pressure control assembly is conducted, the pressure detection assembly and the detection pool are in the same pressure environment, so that the pressure sensor 121 can obtain the pressure value of the detection pool by detecting the pressure value of the pressure control assembly. In a possible implementation, the connecting head 122 is used for interfacing with the front pool of the detection pool, the pressure control assembly is used for providing a preset positive pressure for the front pool, and the pressure sensor 121 is used for detecting the pressure value of the front pool; or, the connecting head 122 is used for interfacing with the rear pool of the detection pool, the pressure control assembly is used for providing a preset negative pressure for the rear pool, and the pressure sensor 121 is used for detecting the pressure value of the rear pool.

[0077] In the process of building pressure for the detection pool by the pressure building mechanism 12, the processor 14 controls the pressure control assembly to build pressure to establish positive pressure or negative pressure. In the process of controlling the pressure control assembly to build pressure, the processor 14 synchronously controls the pressure sensor 121 to detect the pressure of the pressure control assembly, and the processor 14 controls the pressure control assembly to stop building pressure when the pressure value transmitted by the pressure sensor 121 reaches the preset pressure, and controls the pipeline between the pressure control assembly and the connecting head 122 to be conducted, so that the detection mechanism 13 can perform impedance counting on the sample of the detection pool under the preset pressure. In the process of sample detection of the detection mechanism 13, the pressure sensor 121 is also used for continuously detecting the pressure of the detection pool, so that the processor 14 judges whether the air tightness is abnormal based on the pressure detection result.

[0078] In the above manner, the POCT blood cell analyzer of the embodiment can realize the pressure building control before detection, the pressure detection and the air tightness judgment in the detection process through the pressure sensor 121, reduce the problems such as instrument redundancy caused by the addition of new structures, improve the simplicity of the POCT blood cell analyzer and reduce the volume of the POCT blood cell analyzer.

[0079] Optionally, the pressure control assembly comprises a pressure building component 123, a negative pressure component 124, a first control component 125 and a second control component 126, the first end of the first control component 125 is connected with the connector 122, the second end of the first control component 125 is connected with the first end of the negative pressure component 124, the first end of the second control component 126 is connected with the second end of the negative pressure component 124, and the second end of the second control component 126 is connected with the pressure building component 123, the pressure building component 123 is configured to build negative pressure for the negative pressure component 124, so as to provide a preset pressure for the detection pool when the first control component 125 is turned on.

[0080] Specifically, the pressure building component 123 can be, but is not limited to, a syringe or the like that can perform a pressure building operation, the pressure building component 123 is configured to perform a pressure building operation on the negative pressure component 124, and the negative pressure component 124 is configured to store negative pressure. After the connector 122 is connected with the rear pool of the detection pool, the processor 14 is configured to control the second control component 126 to be turned on and control the pressure building component 123 to perform a pressure building operation, and the processor 14 is further configured to perform pressure detection on the negative pressure component 124 through the pressure sensor 121, so as to control the second control component 126 to be cut off and the pressure building component 123 to stop working when the negative pressure component 124 stores a certain negative pressure. After the pressure building operation is completed, the processor 14 is configured to control the first control component 125 to be turned on, so that the negative pressure component 124 is turned on with the rear pool and the rear pool is located in a preset pressure environment, and the sample in the front pool flows to the rear pool under the driving of the negative pressure of the rear pool through the micropore, so that the detection mechanism 13 performs impedance counting on the sample in the sample flowing process.

[0081] In the embodiment of the present application, the processor 14 can switch the states of the first control component 125 and the second control component 126, so that the pressure building mechanism 12 can be reused to build the preset negative pressure of the rear pool and judge the air tightness of the rear pool, reduce the problems such as instrument redundancy caused by the addition of new structures, improve the simplicity of the POCT blood cell analyzer and reduce the volume of the POCT blood cell analyzer.

[0082] In an embodiment, the processor 14 is configured to control the pressure building mechanism 12 to be disconnected with the cartridge 110 when it is determined that the air tightness of the detection pool is abnormal.

[0083] Specifically, when the processor 14 determines that the air tightness of the detection pool is abnormal through the pressure detection result, the processor 14 can be configured to control the first control valve to be cut off and control the bearing mechanism 11 to unload the cartridge 110, so that the connector 122 is disconnected with the cartridge 110; or the processor 14 can also control the bearing mechanism 11 to directly unload the cartridge 110, so that the pressure building mechanism 12 is disconnected with the cartridge 110.

[0084] In the embodiment of the present application, when determining whether the air tightness of the detection pool is abnormal, the processor 14 controls the pressure building mechanism 12 to be disconnected from the reagent box 110, so as to avoid the sample in the detection pool from entering the pipeline through the connecting head 122 when the air tightness is abnormal, thereby reducing the pollution of the connecting head 122 and the pipeline caused by the backflow of the sample, and improving the accuracy and reliability of the POCT blood cell analyzer.

[0085] In an embodiment, the detection pool includes a front pool, a rear pool and a microporous sheet, the microporous sheet is provided with micropores, the front pool is communicated with the rear pool through the micropores of the microporous sheet, and the detection mechanism 13 is configured to perform a counting test on the cells in the sample when the sample in the front pool flows to the rear pool through the microporous sheet. The processor 14 is configured to acquire the flow rate of the sample when the sample flows through the microporous sheet, so as to determine whether the air tightness of the detection pool is abnormal according to the change of the flow rate in the detection process, or the processor 14 is further configured to calculate the flow pressure drop of the sample based on the flow rate, and determine whether the air tightness of the detection pool is abnormal according to the flow pressure drop in the detection process.

[0086] Specifically, the processor 14 can detect the flow rate of the sample when the sample flows through the microporous sheet by using a flow meter, a flow rate meter, a water flow sensor or the like, so as to obtain the flow rate of the sample when the sample flows through the microporous sheet. In an embodiment, since the sample flow is driven by pressure in the detection pool, the pressure of the detection pool changes in a similar trend during the process that the sample in the front pool flows to the rear pool, so that the flow rate of the sample flow gradually decreases in a similar trend. Therefore, the embodiment can determine whether the air tightness of the detection pool is abnormal by using the change of the flow rate, for example, when the flow rate is greatly reduced compared with the normal test process, it is determined that the reagent box 110 is blocked, or when the flow rate is slightly reduced compared with the normal test process, it is determined that the reagent box 110 is leaked. The change value of the flow rate can be obtained by flow rate test at multiple time points, which is not limited herein. The processor 14 can also determine the air tightness by detecting the change of the flow in the detection process. Since the flow is related to the flow rate, details are not described herein.

[0087] In another embodiment, the process of calculating the flow pressure drop of the sample based on the flow rate can be calculated by using the related principle about pressure loss in fluid mechanics, which is not described herein. When the flow rate of the sample flow changes, the flow pressure drop of the sample also changes in a similar way during the process that the sample flows from the side of the microporous sheet close to the front pool to the side of the microporous sheet close to the rear pool. Therefore, the embodiment can also determine whether the air tightness of the detection pool is abnormal by detecting the change of the flow pressure drop in the detection process.

[0088] In the embodiment, the processor 14 can also detect the flow rate of the sample passing through the microporous sheet, so as to determine whether the air tightness of the detection pool is abnormal according to the change of the flow rate in the detection process, or the processor 14 is further configured to calculate the flow pressure drop of the sample based on the flow rate, and determine whether the air tightness of the detection pool is abnormal according to the flow pressure drop in the detection process, so that the reagent box 110 can be processed in time when the air tightness of the detection pool is abnormal, the damage of the POCT blood cell analyzer caused by the air leakage or the hole blockage of the reagent box 110 is reduced, and the detection accuracy and reliability of the POCT blood cell analyzer are improved.

[0089] Optionally, the processor 14 can combine at least one of the flow rate, the flow or the flow pressure drop with the pressure detection result to determine the air tightness, or the processor 14 can determine the air tightness by using at least one of the flow rate, the flow or the flow pressure drop alone, which is not limited herein.

[0090] In an embodiment, the detection mechanism 13 is configured to perform impedance counting on the sample flowing through the microporous sheet when the sample in the front pool flows to the rear pool through the micropores of the microporous sheet. In an embodiment, the detection waste liquid after the impedance counting is stored in the rear pool. In another embodiment, the detection pool further includes a waste liquid pool, the waste liquid pool is in communication with the rear pool, and the pressure building mechanism 12 is configured to be connected with the waste liquid pool to provide a preset pressure for the waste liquid pool. Under the preset pressure of the waste liquid pool, the sample in the front pool flows to the rear pool through the microporous sheet, and the detection waste liquid in the rear pool continues to be driven to the waste liquid pool under the pressure, so that the detection waste liquid is stored in the waste liquid pool.

[0091] Referring to FIG. 6, FIG. 6 is a flowchart of an embodiment of the blood cell analysis method provided by the present application. As shown in FIG. 6, the blood cell analysis method of the embodiment includes the following steps:

[0092] Step S11: receiving the reagent box 110, the detection pool of the reagent box 110 storing the sample to be detected.

[0093] After receiving the reagent box 110, the reagent box 110 is electrically connected with the detection mechanism 13, and the reagent box 110 is also connected with the connecting head 122 of the pressure building mechanism 12.

[0094] Step S12: performing impedance detection on the sample of the reagent box 110.

[0095] In the step S12, the detection mechanism 13 is controlled to perform impedance test on the sample in the detection pool, so that the sample stored in the front pool flows to the rear pool through the micropores, and the detection result of the sample is obtained by acquiring the change of the electric signal when the sample passes through the micropores.

[0096] Step S13: pressure detection is performed on the detection pool during the impedance detection to determine whether the air tightness of the detection pool is abnormal according to the pressure detection result of the pressure building mechanism 12.

[0097] Specifically, pressure detection is performed on the detection pool during the impedance detection, the duration of the pressure detection is within the detection duration of the impedance detection, and the end time of the pressure detection is before the end time of the impedance detection. According to the pressure detection result of the pressure building mechanism 12, it is determined whether the air tightness of the detection pool is abnormal. When it is determined that the air tightness of the detection pool is abnormal, the reagent cartridge 110 can be processed in time, the damage of the POCT blood cell analyzer caused by the reagent cartridge 110 due to air leakage or hole blockage and the like is reduced, and the detection accuracy and reliability of the POCT blood cell analyzer are improved.

[0098] Embodiment One:

[0099] The pressure building mechanism 12 is configured to perform pressure building on the reagent cartridge 110, so that the detection pool of the reagent cartridge 110 is located at a preset pressure of -29 kPa to -31 kPa. The detection mechanism 13 is configured to detect the sample in the detection pool. The pressure building mechanism 12 is further configured to continuously perform pressure detection on the detection pool within 10 s of the detection process of the detection pool to obtain a real-time pressure value. The processor 14 is configured to generate a first warning information when the real-time pressure value is greater than -27 kPa.

[0100] Embodiment Two:

[0101] The pressure building mechanism 12 is configured to perform pressure building on the reagent cartridge 110, so that the detection pool of the reagent cartridge 110 is located at a preset pressure of -29 kPa to -31 kPa. The detection mechanism 13 is configured to detect the sample in the detection pool. The pressure building mechanism 12 is further configured to perform pressure detection on the detection pool at a preset interval (0.1 s to 1 s) within 10 s of the detection process of the detection pool to obtain a pressure change value. The processor 14 is configured to generate a first warning information when the pressure change value is greater than 3 kPa. The processor 14 is further configured to generate a second warning information when the pressure change value is less than 0.1 kPa.

[0102] Embodiment Three:

[0103] The pressure building mechanism 12 is configured to perform pressure building on the reagent cartridge 110, so that the detection pool of the reagent cartridge 110 is located at a preset pressure of -29 kPa to -31 kPa. The detection mechanism 13 is configured to detect the sample in the detection pool. The pressure building mechanism 12 is further configured to perform pressure detection on the detection pool for a preset time period within 10 s of the detection process of the detection pool to obtain a change rate of the pressure value. The processor 14 is configured to generate a first warning information when the change rate is greater than 0.3. The processor 14 is further configured to generate a second warning information when the change rate is less than 0.01.

[0104] Any processes or methods described in the flow charts or otherwise described herein can be understood as representing modules, segments, or portions of code that include one or more executable instructions for implementing specific logic functions or steps, and the preferred embodiments of the present application include additional implementations in which the functions are performed in a different order, in substantially simultaneous fashion, or in reverse order, and the scope of the embodiments of the present application should be understood to include such additional implementations.

[0105] The logic and / or steps represented in the flow charts or otherwise described herein, for example, can be considered as a list of executable instructions for implementing the logic function, and can be embodied in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device, such as a personal computer, server, network device, or other processing device, that can fetch the instructions from the instruction execution system, apparatus, or device, and execute the instructions.

[0106] The above description is merely illustrative of the embodiments of the present application and is not in any way limiting of the patent scope of the present application, and any equivalent structures or equivalent processes transformed by using the content of the specification and drawings of the present application, or directly or indirectly applied to other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A POCT hematology analyzer, wherein, include: A carrier mechanism for loading a reagent kit, wherein the detection cell of the reagent kit stores the sample to be tested; A pressure-building mechanism is connected to the reagent kit on the support mechanism, and the pressure-building mechanism is used to provide a preset pressure to the detection cell; A testing mechanism for testing samples in the testing pool under the preset pressure; The processor is connected to both the pressure-building mechanism and the detection mechanism. The pressure-building mechanism is also used to perform pressure detection on the detection pool during the detection process of the detection mechanism, and the processor is used to determine whether the airtightness of the detection pool is abnormal based on the pressure detection result of the pressure-building mechanism.

2. The POCT blood cell analyzer according to claim 1, wherein, The detection mechanism is used to detect the sample in the detection pool under the preset pressure within a first detection period. The pressure building mechanism performs pressure detection on the detection pool for a second detection period. The second detection period is within the first detection period, and the end time of the second detection period is before the end time of the first detection period.

3. The POCT blood cell analyzer according to claim 1, wherein, The pressure building mechanism is used to perform pressure detection on the detection pool during the detection process to obtain the real-time pressure value of the detection pool. The processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result between the real-time pressure value and a first set threshold.

4. The POCT blood cell analyzer according to claim 3, wherein, The processor is used to generate a first warning message when the real-time pressure value is greater than the first set threshold, so as to indicate that the reagent kit is leaking air through the first warning message.

5. The POCT blood cell analyzer according to claim 1, wherein, The pressure building mechanism is used to perform pressure detection on the detection pool at preset time intervals during the detection process to obtain the pressure change value of the detection pool. The processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the pressure change value and a first threshold range.

6. The POCT blood cell analyzer according to claim 5, wherein, The first threshold range includes a second set threshold and a third set threshold, wherein the third set threshold is greater than the second set threshold; The processor is configured to generate a first warning message when the pressure change value is greater than the third preset threshold, so as to indicate that the reagent kit is leaking air, and / or, the processor is configured to generate a second warning message when the pressure change value is less than the second preset threshold, so as to indicate that the reagent kit is clogged.

7. The POCT blood cell analyzer according to claim 5, wherein, The pressure building mechanism is used to perform pressure detection on the detection pool during the detection process to obtain a first pressure value. The pressure building mechanism is also used to continue to perform pressure detection on the detection pool after a preset time interval to obtain a second pressure value. The processor is used to calculate the difference between the second pressure value and the first pressure value to obtain the pressure change value.

8. The POCT blood cell analyzer according to claim 5, wherein, The pressure change value is the difference between the pressure value of the detection pool during the detection process and the initial pressure value of the detection pool at the start of the test.

9. The POCT hematology analyzer according to claim 1, wherein, The pressure building mechanism is used to perform pressure detection on the detection pool within a preset time period of the detection process to obtain the rate of change of the pressure value of the detection pool within the preset time period. The processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the rate of change and a second threshold range.

10. The POCT hematology analyzer according to claim 9, wherein, The second threshold range includes a fourth set threshold and a fifth set threshold, wherein the fifth set threshold is greater than the fourth set threshold; The processor is configured to generate a first warning message when the rate of change is greater than the fifth preset threshold, so as to indicate that the reagent kit is leaking air, and / or, the processor is configured to generate a second warning message when the rate of change is less than the fourth preset threshold, so as to indicate that the reagent kit is clogged.

11. The POCT blood cell analyzer according to claim 1, wherein, The pressure building mechanism includes a pressure control component, a pressure sensor, and a connector. The connector is disposed on the support mechanism. The pressure control component is connected to the connector and the pressure sensor via pipelines. The connector is used to dock with the reagent kit so that the pressure control component provides a preset pressure to the detection cell. The pressure sensor is used to detect the pressure of the detection cell.

12. The POCT hematology analyzer according to claim 11, wherein, The pressure control assembly includes a pressure-building component, a negative pressure component, a first control component, and a second control component. A first end of the first control component is connected to the connector, a second end of the first control component is connected to the first end of the negative pressure component, a first end of the second control component is connected to the second end of the negative pressure component, and a second end of the second control component is connected to the pressure-building component. The pressure-building component is used to establish negative pressure on the negative pressure component to provide the preset pressure to the detection pool when the first control component is turned on.

13. The POCT blood cell analyzer according to claim 1, wherein, The processor is used to control the pressure building mechanism to disconnect from the reagent kit when it determines that the airtightness of the detection cell is abnormal.

14. The POCT blood cell analyzer according to claim 1, wherein, The detection pool includes a front pool, a rear pool, and a microporous sheet. The front pool is connected to the rear pool through the microporous sheet. The detection mechanism is used to count the cells in the sample when the sample in the front pool flows to the rear pool through the microporous sheet. The processor is used to obtain the flow rate of the sample as it passes through the microporous sheet, so as to determine whether the airtightness of the detection cell is abnormal based on the flow rate change during the detection process. Alternatively, the processor is also used to calculate the flow pressure drop of the sample based on the flow rate, and determine whether the airtightness of the detection cell is abnormal based on the flow pressure drop during the detection process.

15. A method for analyzing blood cells, wherein, include: Receive the reagent kit, wherein the detection cell of the reagent kit stores the sample to be tested; Impedance detection was performed on the samples from the kit. During the impedance detection process, the pressure of the detection cell is measured to determine whether the airtightness of the detection cell is abnormal based on the pressure measurement result of the pressure building mechanism.

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