Salt bush extract composition and its use for skin hydration

Abstract

The present invention relates to a composition including from (i) from about 0.5 to about 10% w / w salt bush extract, at least 5% glycerin, (ii) at least one aqueous solvent, and (iii) at least one emulsifying agent or at least one gelling agent, wherein the composition is intended for use in hydrating skin.

Description

Salt bush extract and uses thereof Field of the invention

[0001] The present invention relates to salt-bush extract compositions suitable for application to the skin, uses thereof and methods of their preparation. Background of the invention

[0002] Recently, along with changes in lifestyle and interest in well-being, there is an increase in demand for anti-aging cosmetic products that can be used to reverse or slow down the development of the visible signs of aging of the skin.

[0003] The skin plays a vital role in protecting against the external environment. This is called the “barrier function” of the skin. The skin barrier protects against many different types of irritating stimuli (eg, chemicals, air pollutants, dryness, UV radiation, etc) and prevents excessive moisture loss. Transepidermal moisture loss (TEWL) increases with age. Increased TEWL can result in the appearance of fine lines that are dehydration lines as opposed to traditional wrinkles due to loss of collagen and elastin in the tissue. One method to protect or repair the skins barrier function is to ensure that skin is hydrated by using a cosmetic composition. Better hydrated skin has improved function, improved feel or comfort level, and improved appearance.

[0004] Cosmetics for improving hydration are known. However, there are limits on the level of hydration achievable by current technologies and products that provide one or more of increased hydration, hydration that penetrates deeper into the skin, or hydration that is longer lasting are in demand.

[0005] There is a need for cosmetic topical formulations that improve hydration levels in skin. In particular, formulations that improve hydration levels in mature skin (eg 40, 45, 50, or 55 years old and over).

[0006] Additionally, consumers are increasingly conscious of the source and nature of the ingredients in their cosmetic products and there is a need to provide cosmetic compositions comprising natural ingredients.

[0007] Reference to any prior art in the specification is not an acknowledgment or suggestion that this prior art forms part of the common general knowledge in any 1006038948jurisdiction or that this prior art could reasonably be expected to be understood, regarded as relevant, and / or combined with other pieces of prior art by a skilled person in the art. Summary of the invention

[0008] The present invention relates to a composition for improving skin conditioning, the composition comprising salt-bush extract as an active ingredient. The composition may improve skin condition and appearance of skin through enhancing skin hydration.

[0009] In one aspect of the disclosure there is provided a composition including (i) from about 0.5 to about 10% w / w salt bush extract, at least 5% glycerin, (ii) at least one aqueous solvent, and (iii) at least one emulsifying agent or at least one gelling agent. The emulsion can be either an oil in water or a water in oil emulsion.

[0010] In embodiments there is provided the composition as herein described wherein the composition further comprises at least one additional emollient and / or at least one additional humectant.

[0011] In embodiments there is provided the composition as herein described wherein the at least one additional emollient is included in an amount from about 10 wt% to about 15 wt% of the total weight of the composition, preferably about 14 wt%.

[0012] In embodiments there is provided the composition as herein described wherein the at least one additional humectant included in an amount from about 5 wt% to about 10 wt% of the total weight of the composition, preferably about 8 wt%.

[0013] In embodiments there is provided the composition as herein described wherein the emollient is selected from the group consisting of: Isononyl Isononanoate; Helianthus Annuus (Sunflower) Seed Oil; Triolein and combinations thereof.

[0014] In embodiments there is provided the composition as herein described wherein the humectant is selected from the group consisting of: Squalane, Pentyl Glycol, Propanediol, Betaine, Sodium Hyalyronate, Hexylene Glycol and combinations thereof.

[0015] In embodiments there is provided the composition as herein described wherein the emulsifying agent is included in an amount from about 1 wt% to about 5 wt%, preferably about 4 wt%. In alternative embodiments there is provided the composition 1006038948as herein described wherein the gelling agent is included in an amount from about 1 wt% to about 5 wt%, preferably about 4 wt%.

[0016] In embodiments there is provided the composition as herein described further comprising at least one antioxidant and / or at least one viscosity modifier and / or at least one skin conditioning agent.

[0017] In embodiments there is provided the composition as herein described wherein the at least one skin conditioning agent in included in an amount of from about 1 wt% to about 10 wt%, preferably from about 2 wt% to about 8 wt%, more preferably about 5 wt%.

[0018] In embodiments there is provided the composition as herein described wherein the viscosity modifier is selected from the group consisting of Magnesium Stearate, Hydrogenated Vegetable Oil and combinations thereof.

[0019] In embodiments there is provided the composition as herein described wherein the at least one skin conditioning agent is selected from Jojoba Seed Oil, Santalum Spicatum Seed Oil (Sandalwood Seed Oil), Macadamia Seed Oil esters, Phytosteryl Macadamiate Pytosterols, Tocopherol and combinations thereof.

[0020] In embodiments viscosity modifier is included in an amount from about 1 wt% to about 5 wt%, preferably about 3 wt%. In embodiments the emulsifier is selected from polyglyceryl-4 Oleate, polyglyceryl-6 Oleate, polyhydroxystearic acid and combinations thereof.

[0021] In embodiments the composition further includes a peptide, preferably selected from the group consisting of Acetyl Hexapeptide-8, Palmitoyl Tripeptide-8 and or combinations thereof.

[0022] In embodiments the composition further includes an environmental protectant, preferably Ectoin.

[0023] In embodiments the antioxidant is ascorbyl tetraisopalmitate.

[0024] In embodiments the salt bush extract is present in a concentration of from about 1% w / w about 10% w / w. In alternative embodiments the salt bush extract is present in a concentration of from about 0.5% w / w to 5% w / w. In alternative embodiments the salt 1006038948bush extract is present in a concentration of from about 0.5% w / w to 7% w / w, preferably from about 1% w / w to 5% w / w.

[0025] In embodiments the salt bush extract is present in a concentration of about 5% w / w. In embodiments the salt bush extract is present in a concentration of about 1% w / w.

[0026] In embodiments of the disclosure as herein described, salt bush extract is Australian salt bush extract, and / or old man saltbush extract, and / or Atriplex nummularia extract

[0027] In embodiments of the disclosure there is provided a composition including one or more of: from about 0.5 wt% to about 10 wt% salt bush extract; from about 5 wt% to about 10 wt% glycerin; from about 5 wt% to about 15 wt% of at least one additional emollient; from about 5 wt% to about 10 wt% of at least one additional humectant; from about 1 wt% to about 5 wt% of at least one emulsifying agent; from about 1 to about 10 wt% of at least one skin conditioning agent; from about 1 to about 5 wt% of a viscosity modifier; and and aqueous solvent, wherein the solvent makes up the balance of the composition. Optionally, the composition includes all of these options.

[0028] In embodiments there is provided a composition as herein described further including one or more of from about 0.1 to about 2% of at least one antioxidant; from about 0.5 to about 4% of one or more peptides; and from about 0.1 wt% to about 1 wt% of at least one environmental protectant. 1006038948

[0029] In another aspect of the disclosure there is provided a method of hydrating the skin comprising applying to the skin a composition comprising the composition as herein described.

[0030] In embodiments the skin shows an increase in hydration of about 10%, or about 15%, or about 20% or about 25% or about 30% 3 hours after application.

[0031] In embodiments the composition comprises at least 1% salt bush extract and the skin shows an increase in hydration of about 20% 3 hours after application. Alternatively the composition comprises at least 5% salt bush extract and the skin shows an increase in hydration of about 25% or about 30% 3 hours after application.

[0032] In embodiments the composition is applied to the skin on the face, neck or décolletage. In alternative embodiments the composition is applied to the skin on the body below the neck, including the hands. In embodiments the composition is applied once per day. Optionally, 0.5 to 1 g of the composition is applied to the face, and / or to the neck and décolletage.

[0033] In embodiments the skin is mature skin, for example the person applying the composition is at least 40 years old, or the person is from 40 to 60 years old, or from 45 to 55 years old, or is at least 50 years old.

[0034] Further aspects of the present invention and further embodiments of the aspects described in the preceding paragraphs will become apparent from the following description, given by way of example and with reference to the accompanying drawings. Brief description of the drawings

[0035] Figure 1 shows a graph with the results of a hydration study comparing the hydration effect of a cream comprising (a) salt bush extract compared to a negative control and (b) salt bush extract compared to a positive control. Detailed description of the embodiments

[0036] It will be understood that various terms employed in the specification, examples and claims have meanings that will be understood by one of ordinary skill in the art. However, certain terms are defined below. 1006038948

[0037] It will be understood that the invention disclosed and defined in this specification extends to all alternative combinations of two or more of the individual features mentioned or evident from the text or drawings. All of these different combinations constitute various alternative aspects of the invention.

[0038] As used herein, the term “room temperature” refers to about 20 °C.

[0039] As used herein, the term “standard humidity” refers to 30 to 50% relative humidity. Optionally, 40% relative humidity.

[0040] The term “skin” as used herein refers to the tissue covering the surface of the body in animals. It may be used in a very inclusive sense to include scalp in addition to the tissue covering the surface of the face or body. Preferably, the term “skin” refers to dermal skin, that is skin containing a dermal layer as opposed to mucus membranes.

[0041] The term “extract” as used herein refers to all the substances obtained by extraction of natural components (such as components of salt bush) irrespective of the extraction method, the extracting solvent, the extracted component, or the form of the extracted substance. Preferably, the extract is a cold extract such as an extraction at 4- 30 °C. This is expected to avoid heat deactivation or denaturisation of the proteins and enzymes from the plant. An alternative method to preserve protein and enzyme function is also acceptable. Preferably, the extraction is conducted under pressure, for example from 10 to 1,000 MPa, optionally under high pressure, from 100 MPa to 600 MPa. This can increase the speed of extraction. Preferably, the extraction is both cold and under pressure. Preferably, the extract is of the whole salt bush, the whole salt bush excluding roots, or salt bush leaves. Preferably, the salt bush and / or salt bush leaves are roughly chopped, shredded or otherwise broken into smaller pieces before extraction. Preferably, extraction used water, glycerin or a combination thereof as the extraction solvent. Preferably, extraction is conducted in 30 minutes to 3 hours.

[0042] As used herein, the term "administration" means introducing the composition of the present invention to a patient by any suitable method. Since the composition of the present invention is effective in improving skin wrinkles, the route of administration of the composition may be administered by applying to the skin. Preferably, administration is topical administration. Preferably, topical administration to the skin. 1006038948

[0043] As used herein, except where the context requires otherwise, the term "comprise" and variations of the term, such as "comprising", "comprises" and "comprised", are not intended to exclude further additives, components, integers or steps. Salt bush extract

[0044] As used herein, desert saltbush extract is an extract of Atriplex nummularia, also known as Australian saltbush or old man saltbush.

[0045] Australian salt-bush extract, is known to have nutritional and medicinal properties. It is a small hardy Australian native plant that can withstand dry salt soils and grows in the semi-arid and arid regions of mainland Australia. The largest species of Australian salt bush is known as “Atriplex nummularia”.

[0046] Desert Saltbush, or Atriplex-nummularia, contains potent compounds beneficial for skin health including antioxidants; high mineral content including calcium, magnesium, iron and potassium; unique phytochemicals including terpenoids, phenolics, flavonoids, isorhamnetin, organic acids and amino acids and beta-carotene.

[0047] Antioxidants are known to be beneficial in cosmetic compositions as they can neutralize free radicals, which are known to contribute to skin aging.

[0048] Desert salt-bush extract has been found to be particularly suitable for boosting skin hydration. As a desert plant, salt bush is adapted to retain moisture. Hydration

[0049] Hydration of the skin refers to the amount of water in the skin. Ingredients and formulations that increase hydration prevent loss of water (for example, prevent TEWL) and retain water in the skin and / or deliver additional water to the skin by facilitating increased water absorption.

[0050] Hydration is different to moisturisation, which is a measure of the amount of oil in skin.

[0051] Maintaining skin hydration can prevent dryness and give the skin a plumper and more youthful appearance. This could be particularly beneficial for dry and dehydrated 1006038948skin types, providing a boost of hydration and helping to maintain the skin’s natural barrier.

[0052] Skin hydration may be measured using a skin surface hydration meter, a Skin Corneometer or other suitable device. Other ingredients

[0053] As used herein the term emulsifier refers to an agent or ingredient in a composition which promotes blending of oil and water phases into an emulsion. Emulsifiers are typically surfactants and may be a non-ionic, cationic, anionic or zwitterionic emulsifier. The emulsifier may be an oil in water emulsifier or a water in oil emulsifier. The emulsifier may be used with a co-emulsifier to enhance stability of the emulsion. Non-limiting examples of emulsifiers include esters of glycerin, esters of propylene glycol, fatty acid esters of polyethylene glycol, fatty acid esters of polypropylene glycol, esters of sorbitol, esters of sorbitan anhydrides, carboxylic acid copolymers, esters and ethers of glucose, ethoxylated ethers, ethoxylated alcohols, alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acid amides, acyl lactylates, soaps, TEA stearate, DEA oleth-3 phosphate, polyethylene glycol 20 sorbitan monolaurate (polysorbate 20), polyethylene glycol 5 soya sterol, steareth-2, steareth-20, steareth-21, ceteareth-20, PPG-2 methyl glucose ether distearate, ceteth-10, polysorbate 80, cetyl phosphate, potassium cetyl phosphate, diethanolamine cetyl phosphate, polysorbate 60, glyceryl stearate, PEG-100 stearate, lecithin, and mixtures thereof.

[0054] Optionally the emulsifier is selected from lecithin, polyglyceryl-4 Oleate, polyglyceryl-6 Oleate, polyhydroxystearic acid and combinations thereof.

[0055] As used herein a gelling agent, or gellant, is an agent which promotes gel-like texture in a formulation. Gelling agents help provide desired consistency, viscosity and stability to cosmetics creams, lotions and gels. Gelling agents may be also used as thickeners. Typical gelling agents include but are not limited to guar gum, xantham gum, gellam gum, locust bean gum, acacia gum, vegetable gum, tragacanth, hydroxyethyl cellulose, methyl cellulose, hydroxypropyl methylcellulose, carboxymethyl cellulose, polysaccharides, agar-agar, alginic acid or alginates, pectin, gelatine, polyacrylic acid and derivatives thereof for example sodium acrylate, chitosan, polyethylene glycol, 1006038948polyethylene oxide (PEO), polypropylene oxide (PPO) and PEO-PPO co-polymers, and modified starch.

[0056] The gelling agent may be a combination of one or more gelling agents or a combination of a gelling agent and an emulsifier. For example, in some embodiments the gelling agent is LecigelTMa mixture of sodium acrylate copolymer and lecithin.

[0057] As used herein the term emollient is an agent, or ingredient in a composition which can contribute to skin hydration. Emollients may also be known as barrier creams because they form a film on the skin’s outer layer which can help to prevent TEWL.

[0058] Common emollients are glycerin, lanolin, cocoates, synthetic esters, fatty acids, cocoa butter, cocoa oil, vegetable oils, mineral oil and petroleum jelly.

[0059] In some embodiments the composition comprises one or more emollients selected from alkyl esters, vegetable oils, triglycerides and combinations thereof.

[0060] As used herein a humectant is an agent or ingredient that attracts water. Humectants are used in cosmetic formulations as they can help hydrate the skin, hair or nails.

[0061] Commonly used humectants are Hyaluronic acid (or salts thereof), glycerin and alkyl glycols.

[0062] In some embodiments the composition comprises one or more humectants selected from saturated hydrocarbon oil, alkyl glycol, hyaluronic acid or derivative thereof, an amino acid (betaine) or a combination thereof.

[0063] Some ingredients, for example glycerin, function as both an emollient and a humectant.

[0064] The compositions of the invention may be particularly suitable for mature skin. Typically, a person who is 40 years or older may be considered to have mature skin. However, sun exposure and lifestyle factors may contribute to the maturing of skin at younger ages. Characteristics of mature skin include a slower rate of cell turnover, loss of collagen and hyaluronic acid and reduced sebum secretion which results in an increase in dryness and dehydration and a reduction in elasticity and firmness of the 1006038948skin. Of specific relevance to this invention, mature skin tends to be more dehydrated, have greater TEWL and need greater amounts of hydration to younger skin.

[0065] In some embodiments of the invention the composition is applied to the skin of an adult who is older than 30 years, older than 40 years, older than 50 years or older than 60 years. Examples Example 1 Comparative testing of hydration efficacy

[0066] Glycerin is a natural humectant and has been used as a standard in hydration in skincare for many years. It is a humectant that helps to keep the skin hydrated and reduce TEWL (trans epidermal water loss). This study was designed to determine if addition of saltbush extract to a formulation containing glycerin will increase hydration of a formulation compared to formulations including only glycerin. Ingredients

[0067] The salt bush extract used in this and the following experiments was purchased from Native Extracts (www.nativeextracts.com) and prepared using their cellular extraction process at low temperature and under pressure and in an airless environment. It is currently for sale as Salt bush cellular extract, Atriplex nummularia. Method

[0068] A basic chassis cream was developed with emollient (10%), humectant(glycerin) and various levels of saltbush (0,1 and 5%). The formulation of the chassis cream is shown in table 4 below. The purpose of the experiment was to determine if adding saltbush extract increases the immediate and short term hydration when compared to glycerin alone. Table 1- Chassis cream formulation Ingredient wt% Aqua balance Caprilyic / Capric Triglycerides 10 Glycerin 5 1006038948NE Saltbush Extract 0, 1 or 5 wt% Lecigel 1 Preservative 1

[0069] 4 subjects with dry skin were tested using a corneometer (Corneometer CM825 sold by Courage + KHAZAKA Electronic GmbH). The subjects were tested on the inner forearm. Formulation was added to 4 spots on the arm and tested initially prior to addition, at 20 minutes, 60 minutes and 3 hours.

[0070] The subjects were a variety of ages – 77 years, 55 years, 17 years and 54 years. Results

[0071] Skin hydration has great variability and can be dependent on age, lifestyle, temperature, and many other factors. Initial results on 4 subjects, indicate that the addition of salt bush extract has an improvement in hydration of a formulation over glycerin on its own. The results were measured after 3 hours and have been recorded below in tables R1 and R2. The % increase is measured against a baseline of untreated skin (no cream applied). Without being bound by theory, it is expected that the higher percentage increases in hydration occurred in subjects with more dehydrated skin. Table R1. % Increase in hydration after 3 hours % Increase in Hydration 3H over negative control 5% Glycerin + 5% Glycerin + 5% Glycerin 1% Saltbush 5% Saltbush Subject 1 8.34 11.62 13.78 Subject 2 32.90 39.30 46.80 Subject 3 12.80 30.10 30.20 Subject 4 11.30 4.00 24.50 Average 16.34 21.26 28.82 SD 0.11 0.16 0.14 1006038948A second experiment was conducted to compare the hydration efficacy of a formulation comprising salt-bush extract with no glycerin with pure glycerin. Table R2. % Increase in hydration from Saltbush inclusion versus pure glycerin % Increase in Hydration 3H over 5% glycerin positive control 1% Saltbush 5% Saltbush Subject 1 39.30 65.00 Subject 2 19.60 42.50 Subject 3 135.20 135.90 Subject 4 Not measured 117.50 Average 64.70 81.13 SD 0.62 0.44

[0072] Microsoft excel was used to calculate the percentage mean and standard deviation for each subject and the resulting data was plotted against the percentage increase in hydration after 3 hours (Figure 1). Results summary

[0073] 100% of subjects showed an increase in hydration with all 3 formulations.

[0074] 100% of subjects showed a larger increase in hydration with the formulation containing 5% saltbush over the glycerin only control.

[0075] 75% of subjects (all with results) showed a larger increase in hydration with the formulation containing 1% saltbush over that of glycerin only.

[0076] These results indicate that formulations including both saltbush extract and glycerin improve skin hydration compared to formulations with only glycerin. Example 2- Composition 1

[0077] Table 2 below shows the formulation of composition 1. Table 2 – Formulation of composition 1 Raw Material Ingredient (INCI) Name CAS # Function RM Name (RM) concentration (%)* 1006038948Magnesium Magnesium Stearate 557-04-0 Viscosity Modifier 0.75 Stearate Lanoll 99 Isononyl Isononanoate 42131-25-9 Emollient 7.7 Cetiol CC Dicaprylyl Carbonate 1680-31-5 Skin conditioner 3.55 and emollient Polyaquol V04 Polyglyceryl-4 Oleate (and) 58128-22-6 Emulsifier 4 Polyglyceryl-6 Oleate (and) 9007-48-1 Polyhydroxystearic Acid Florasun 90 Helianthus Annuus 8001-21-6 Emollient 2 (Sunflower) Seed Oil Australian Jojoba Simmondsia Chinensis 61789-91-1 Skin conditioner 2 Oil (Jojoba) Seed Oil Algapur Triolein 122-32-7 Skin conditioner 0.5 and emollient Squalane Squalane 111-01-3 Humectant 1 Sandalwood Santalum 8024-35-9 Skin conditioner 1 Seed Oil Spicatum Seed Oil L22 Jojoba Oil / Macadamia Seed 111-02-4 Skin conditioner 1.5 Oil Esters (and) 68990-51-2 Squalene (and) Phytosteryl 97593-46-9 Macadamiate (and) 68555-08-8 Phytosterols (and) 1406-18-4 Tocopherol Viscolid Hydrogenated Vegetable Oil 68334-28-1 Thickener 2.5 Pentylene Glycol Pentylene Glycol 5343-92-0 Humectant and 2 Preservative Booster Propanediol Propanediol 504-63-2 Humectant, 2 Preservative Booster and Active Enhancer Hexiol Hexylene Glycol 6920-22-5 Preservative and 0.5 Humectant Glycerin Glycerin 56-81-5 Humectant, 5 emollient Aqua Aqua 7732-18-5 Solvent 54 Water (and) 7732-18-5 Peptide 2 1006038948Argirilene Acetyl Hexapeptide-8 (and) 616204-22-9 Amplified Sodium Benzoate 532-32-1 Nutrazen Water (and) Butylene Glycol 7732-18-5 Peptide 2 (and) 107-88-0 Dextran (and) Palmitoyl 9004-54-0 Tripeptide-8 936544-53-5 Betaine Betaine 107-43-7 Humectant 2 Sodium Chloride 7647-14-5 Stabiliser 1 Salt Bush Extract Atriplex nummalaria Leaf Skin conditioner 1 Extract Ectoin Ectoin 96702-03-3 Environmental 0.5 protectant Cube 3 Sodium Hyaluronate 9067-32-7 Humectant, skin 0.5 conditioner VCIP Ascorbyl Tetraisopalmitate 183476-82-6 Antioxidant 1 Total 100

[0078] The same composition is described in Table 3 below with the ingredients ordered by function. 1006038948Table 3 – functional composition of composition 1 Raw Material Ingredient (INCI) CAS # Function RM Ingredient Name (RM) Name concentration (%) (%)* Aqua Aqua 7732-18-5 Solvent 54 54 Salt Bush Atriplex Skin conditioner 1 1 Extract nummalaria Leaf Extract Glycerin Glycerin 56-81-5 Humectant, 5 5 emollient Lanoll 99 Isononyl 42131-25-9 Emollient 7.7 13.75 Isononanoate Cetiol CC Dicaprylyl 1680-31-5 3.55 Carbonate Florasun 90 Helianthus Annuus 8001-21-6 2 (Sunflower) Seed Oil Algapur Triolein 122-32-7 0.5 Squalane Squalane 111-01-3 Humectant 1 8 Pentylene Pentylene Glycol 5343-92-0 2 Glycol Propanediol Propanediol 504-63-2 2 Betaine Betaine 107-43-7 2 Cube 3 Sodium 9067-32-7 0.5 Hyaluronate Hexiol Hexylene Glycol 6920-22-5 0.5 Australian Simmondsia 61789-91-1 Skin conditioner 2 4.5 Jojoba Oil Chinensis (Jojoba) Seed Oil Sandalwood Santalum 8024-35-9 1 Seed Oil Spicatum Seed Oil L22 Jojoba 111-02-4 1.5 Oil / Macadamia 68990-51-2 Seed Oil Esters 97593-46-9 (and) 68555-08-8 1406-18-4 1006038948Squalene (and) Phytosteryl Macadamiate (and) Phytosterols (and) Tocopherol Polyaquol V04 Polyglyceryl-4 58128-22-6 Emulsifier 4 4 Oleate (and) 9007-48-1 Polyglyceryl-6 Oleate (and) Polyhydroxystearic Acid Magnesium Magnesium 557-04-0 Viscosity 0.75 3.25 Stearate Stearate Modifier Viscolid Hydrogenated 68334-28-1 2.5 Vegetable Oil Argirilene Water (and) 7732-18-5 Peptide 2 4 Amplified Acetyl 616204-22-9 Hexapeptide-8 532-32-1 (and) Sodium Benzoate Nutrazen Water (and) 7732-18-5 2 Butylene Glycol 107-88-0 (and) 9004-54-0 Dextran (and) 936544-53-5 Palmitoyl Tripeptide-8 Sodium 7647-14-5 Stabiliser 1 1 Chloride Ectoin Ectoin 96702-03-3 Protectant 0.5 0.5 against environmental stresses VCIP Ascorbyl 183476-82-6 Antioxidant 1 1 Tetraisopalmitate Total 100 100 Method of formulation: 1006038948

[0079] The formulation in the table below was made using the method of Table 4 below. Table 4 – Formulation method of composition 1 Phase A Ingredient INCI Wt% Supplier A Magnesium Stearate Magnesium Stearate 0.75 New Directions A ININ 100KC Isononyl Isononanoate 7.70 Ingredients Plus (KCI Limited) A Indiester DOC MB Dicaprylyl Carbonate 3.55 Ingredients Plus (Sina BT Co., Ltd) A Polyaquol V04 Polyglyceryl-4 Oleate (and) 4.00 Innovacos Polyglyceryl-6 Oleate (and) Polyhydroxystearic Acid A Florasun 90 Helianthus Annuus 2.00 Avenir (Cargill) (Sunflower) Seed Oil A Australian Jojoba Oil Simmondsia Chinensis 2.00 New Directions (Jojoba) Seed Oil A Algapur HSHO Triolein 0.50 Lubrizol A Neossance Squalane Squalane 1.00 Trapeze (Amyris) A Sandalwood Seed Oil Santalum Spicatum Seed Oil 1.00 Native Extracts A L22 Jojoba Oil / Macadamia Seed 1.50 Avenir (Cargill) Oil Esters (and) Squalene (and) Phytosteryl Macadamiate (and) Phytosterols (and) Tocopherol A Dermofeel Viscolid MB Hydrogenated Vegetable Oil 2.50 Avenir (Evonik) A Tocobest Vitamin E Tocopherol 1.00 Ingredients Plus 1300IU (Kangcare) B Hydrolite 5 Pentylene Glycol 2.00 Ingredients Plus (Symrise) B Tilamar PDO / Zemea Propanediol 2.00 Brenntag Propanediol (DSM) / IMCD(Cova tionBio PDO LLC) B Hydrolite 6 Hexylene Glycol 0.50 Ingredients Plus (Symrise) B Glycerin Glycerin 5.00 Any B Water Aqua 52.00 Any 1006038948B Genecare OSMS BA Betaine 2.00 Connell Brothers (IFF's Health & Biosciences) B Sodium Chloride Sodium Chloride 1.00 Any B Ronacare Ectoin Ectoin 0.50 Azelis (Merck) B Primalhyal Cube 3 Sodium Hyaluronate 0.50 Carst and Walker (Roelhmi) C Argirilene Amplified Water (and) Acetyl 2.00 Lubrizol Hexapeptide-8 (and) Sodium Benzoate C Nutrazen Water (and) Butylene Glycol 2.00 Avenir (Lucas (and) Dextran (and) Palmitoyl Meyer) Tripeptide-8 C NE Salt Bush Extract Atriplex nummalaria Leaf 1.00 Native Extracts Conc Extract C VC-IPVS Ascorbyl Tetraisopalmitate 1.00 CeeChem (Nikkol) C Niacinamide Niacinamide 1.00 New Directions Total 100.00 Step 1. The phase A ingredients were weighed into suitable vessel and heated to 70°C while stirring until all waxes had melted. Step 2. The phase B ingredients were weighed into separate vessel and heated to 70°C while stirring. Stirring was continued until the ingredients formed a uniform mixture. Step 3. Keeping both phase A and B at temperature, the phase B mixture was slowly added phase A under continual stirring. Step 4. The mixture was homogenised for 2 minutes. The mixture was cooled to 50°C while stirring. Step 5. The Phase C ingredients were added to the mixture and the mixture was homogenised for 5 minutes. Step 7. The mixture was cooled to 40°C under slow stirring. 1006038948

[0080] The product produced by the above method is a uniform, viscous, white water in oil emulsion. The product may be also known as a white water in oil cream. Example 3 - Normal Use

[0081] Composition 1 is applied in an amount of about 0.5-1 g per application to the face by gently spreading across the surface with fingers. The product is left on the skin. The product is used once per day. Similar amounts and use are applicable for the neck and décolletage. 1006038948

Claims

CLAIMS 1. A composition including from (i) from about 0.5 to about 10% w / w salt bush extract, at least 5% glycerin, (ii) at least one aqueous solvent, and (iii) at least one emulsifying agent or at least one gelling agent.

2. The composition of claim 1 wherein the composition further comprises at least one additional emollient and / or at least one additional humectant.

3. The composition of claim 2 wherein the at least one additional emollient is included in an amount from about 10 wt% to about 15 wt% of the total weight of the composition, preferably about 14 wt%.

4. The composition of claim 2 or 3 wherein the at least one additional humectant included in an amount from about 5 wt% to about 10 wt% of the total weight of the composition, preferably about 8 wt%.

5. The composition of any one of claims 2 to 4 wherein the emollient is selected from the group consisting of: Isononyl Isononanoate; Helianthus Annuus (Sunflower) Seed Oil; Triolein and combinations thereof.

6. The composition of any one of claims 2 to 5 wherein the humectant is selected from the group consisting of: Squalane, Pentyl Glycol, Propanediol, Betaine, Sodium Hyalyronate, Hexylene Glycol and combinations thereof.

7. The composition of any one of claims 1 to 6 wherein the emulsifying agent or gelling agent is included in an amount from about 1 wt% to about 5 wt%, preferably about 4 wt%.

8. The composition of any one of claims 1 to 7 further comprising at least one antioxidant and / or at least one viscosity modifier and / or at least one skin conditioning agent.

9. The composition of claim 8 wherein the at least one skin conditioning agent in included in an amount of from about 1 wt% to about 10 wt%, preferably from about 2 wt% to about 8 wt%, more preferably about 5 wt%. 100603894810. The composition of claim 8 or 9 wherein the viscosity modifier is selected from the group consisting of Magnesium Stearate, Hydrogenated Vegetable Oil and combinations thereof.

11. The composition of any one of claims 8 to 10 wherein the at least one skin conditioning agent is selected from Jojoba Seed Oil, Santalum Spicatum Seed Oil (Sandalwood Seed Oil), Macadamia Seed Oil esters, Phytosteryl Macadamiate Pytosterols, Tocopherol and combinations thereof.

12. The composition of any one of claims 8 to 12 wherein the viscosity modifier is included in an amount from about 1 wt% to about 5 wt%, preferably about 3 wt%.

13. The composition of any one of the preceding claims, wherein the emulsifier is selected from polyglyceryl-4 Oleate, polyglyceryl-6 Oleate, polyhydroxystearic acid and combinations thereof.

14. The composition of any one of the preceding claims, wherein the composition further includes a peptide, preferably selected from the group consisting of Acetyl Hexapeptide-8, Palmitoyl Tripeptide-8 and or combinations thereof.

15. The composition of any one of the preceding claims, wherein the composition further includes an environmental protectant, preferably Ectoin.

16. The composition of any one of claims 8 to 15, wherein the antioxidant is ascorbyl tetraisopalmitate.

17. The composition of any one of the preceding claims wherein the salt bush extract is present in a concentration of from about 1% w / w about 10% w / w.

18. The composition of any one of claims 1 to 16 wherein the salt bush extract is present in a concentration of from about 0.5% w / w to 5% w / w.

19. The composition of any one of claims 1 to 18 wherein the salt bush extract is present in a concentration of from about 1% w / w to 5% w / w.

20. The composition of any one of claims 1 to 18 wherein the salt bush extract is present in a concentration of from about 0.5% w / w to 7% w / w. 100603894821. The composition of any one of claims 1 to 20 wherein the salt bush extract is present in a concentration of about 5% w / w.

22. The composition of any one of claims 1 to 20 wherein the salt bush extract is present in a concentration of about 1% w / w.

23. The composition of any one of the preceding claims wherein the salt bush extract is Australian salt bush extract, or old man saltbush extract.

24. The composition of any one of the preceding claims wherein the salt bush extract is Atriplex nummularia extract.

25. A composition including: from about 0.5 wt% to about 10 wt% salt bush extract; from about 5 wt% to about 10 wt% glycerin; from about 10 wt% to about 15 wt% of at least one additional emollient; from about 5 wt% to about 10 wt% of at least one additional humectant; from about 1 wt% to about 5 wt% of at least one emulsifying agent or gelling agent; from about 1 to about 10 wt% of at least one skin conditioning agent; from about 1 to about 5 wt% of a viscosity modifier; and and aqueous solvent, wherein the solvent makes up the balance of the composition.

26. The composition of claim 19 further including from about 0.1 to about 2% of at least one antioxidant; from about 0.5 to about 4% of one or more peptides; and from about 0.1 wt% to about 1 wt% of at least one environmental protectant. 100603894827. The composition of any of the claims 1 to 26, wherein the formulation includes an emulsifying agent and is an oil in water emulsion.

28. The composition of any of the claims 1 to 26, wherein the formulation includes an emulsifying agent and is a water in oil emulsion.

29. The composition of any of the claims 1 to 28, wherein the formulation includes a gelling agent.

30. The composition of any of the claims 1 to 29, wherein the extract is a cold extract such as an extract prepared by extraction at 4-30 °C.

31. The composition of any of the claims 1 to 30, wherein the extraction is conducted under pressure, optionally 10 to 1,000 MPa or 100 MPa to 600 MPa.

32. The composition of any of the claims 1 to 31, wherein the extract is of the whole salt bush, the whole salt bush excluding roots, or salt bush leaves.

33. The composition of any of the claims 1 to 32, wherein the salt bush is roughly chopped, shredded or otherwise broken into smaller pieces before extraction.

34. The composition of any of the claims 1 to 33, wherein the extract is extracted in water, glycerin or a combination thereof.

35. A method of hydrating the skin comprising applying to the skin a composition comprising the composition of any one of claims 1 to 34.

36. The method of claim 35 wherein the skin shows an increase in hydration of about 10%, or about 15%, or about 20% or about 25% or about 30% 3 hours after application.

37. The method of claim 35 wherein the composition comprises at least 1% salt bush extract and the skin shows an increase in hydration of about 20% 3 hours after application.

38. The method of claim 35 wherein the composition comprises at least 5% salt bush extract and the skin shows an increase in hydration of about 25% or about 30% 3 hours after application. 100603894839. The method of any one of claims 35 to 38 wherein the composition is applied to the skin on the face, neck or décolletage.

40. The method of any one of claims 35 or 39 wherein the composition is applied once per day.

41. The method of any one of claims 35 to 40 wherein the skin is mature skin.

42. The method of claim 41 wherein the person applying the composition is at least 40 years old.

43. The method of claim 41 or 42 wherein the person is from 40 to 60 years old, or from 45 to 55 years old, or is at least 50 years old.

44. The method of any one of claims 35 to 43, wherein 0.5 to 1 g of the composition is applied to the face, and / or to the neck and décolletage. 1006038948

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