Systems and methods for digital detection assays

Abstract

Disclosed herein are systems and methods for multiplexed nucleic acid amplification assays and multi-parallel immunoassays using a set of color codes for detection of nucleic acids and antigens. Kits and software for implementing the systems and methods are also disclosed.

Description

Docket No. ABBTT-43596.601 SYSTEMS AND METHODS FOR DIGITAL DETECTION ASSAYS RELATED APPLICATION INFORMATION This application claims priority to U.S. Application No.63 / 674,136, filed on July 22, 2024, and U.S. Application No.63 / 680,856, filed on August 8, 2024, the contents of each of which are herein incorporated by reference. TECHNICALFIELDThe present disclosure relates to systems and methods for digital multiplexed nucleic acid amplification assays and digital multi-parallel immunoassays using a set of color codes for detection of nucleic acids and antigens of interest. Kits and software for implementing the systems and methods are also disclosed. BACKGROUND Immunoassays using a non-digital multiplex format face significant challenges due to reliance on dye combinations for detecting protein antigens in capture assays. This method often encounters issues such as dye interference and limited multiplexing capability, complicating the accurate and efficient measurement of multiple proteins simultaneously. Nucleic acid amplification assays face similar challenges. These challenges underscore the need for improved multiplexed and multi-parallel digital assays for detecting analytes in biological samples. SUMMARY Aspects of the present disclosure relate to systems and methods for multiplexed and multi- parallel digital detection assays for detecting analytes of interest in biological samples. In an embodiment, the present disclosure relates to a method for performing a digital multiplexed nucleic acid amplification assay to detect a plurality of target nucleic acids in a sample. The method comprises: (a) labeling at least three different nucleic acid probes with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a 1Docket No. ABBTT-43596.601 combination of dyes comprising the first color label or dye and the second color label or dye; (b) aliquoting the sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel (many reaction vessels will be empty) contains a single molecule of target nucleic acid and the at least three different nucleic acid probes; (c) amplifying in each reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (iii) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel. In some embodiments of the above method, step (a) comprises labeling at least six different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least six different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; and (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye. In some embodiments of the above method, (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye 2Docket No. ABBTT-43596.601 when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the fifth target nucleic acid is detected in the reaction vessel; and (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the target nucleic acid is detected in the reaction vessel. In some embodiments of the above method, step (a) comprises labeling at least ten different nucleic acid probes with at least four different colored labels or dyes to create a set of color codes specific for at least ten different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of dyes comprising the first color label or dye and the second color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (viii) an eight nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; and (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a 3Docket No. ABBTT-43596.601 combination of labels or dyes comprising the third color label or dye and the fourth color label or dye. In some embodiments of the above method: In still further embodiments of the above method: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; and (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel. In still further embodiments of the above method, step (a) comprises labeling at least 15 different nucleic acid probes with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; 4Docket No. ABBTT-43596.601 (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (ix) a ninth nucleic acid probe specific for an ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; and (xv) a fifteenth nucleic acid probe specific for a fifteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye. In still further embodiments of the above method, (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; 5Docket No. ABBTT-43596.601 (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fifth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the tenth target nucleic acid is detected in the reaction vessel; (xi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the eleventh target nucleic acid is detected in the reaction vessel; (xii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fifth color label or dye when the twelfth target nucleic acid is detected in the reaction vessel; (xiii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the thirteenth target nucleic acid is detected in the reaction vessel; (xiv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fifth color label or dye when the fourteenth target nucleic acid is detected in the reaction vessel; and (xv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye when the fifteenth target nucleic acid is detected in the reaction vessel. 6Docket No. ABBTT-43596.601 In still other embodiments of the above method, step (a) comprises labeling at least 21 different nucleic acid probes with at least six different colored labels or dyes to create a set of color codes specific for at least 21 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a sixth color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the sixth color label or dye; (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xv) a fifteenth nucleic acid probe specific for a fifteenth target nucleic acid is labeled with a 7Docket No. ABBTT-43596.601 combination of labels or dyes comprising the second color label or dye and the sixth color label or dye; (xvi) a sixteenth nucleic acid probe specific for a sixteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xvii) a seventeenth nucleic acid probe specific for a seventeenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; (xviii) an eighteenth nucleic acid probe specific for an eighteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the sixth color label or dye; (xix) a nineteenth nucleic acid probe specific for a nineteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye; (xx) a twentieth nucleic acid probe specific for a twentieth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye; and (xxi) a twenty-first nucleic acid probe specific for a twenty-first target nucleic acid is labeled with a combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye. In some embodiments of the above method: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the sixth color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the seventh target nucleic acid is detected in the reaction vessel; 8Docket No. ABBTT-43596.601 (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fifth color label or dye when the tenth target nucleic acid is detected in the reaction vessel; (xi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the sixth color label or dye when the eleventh target nucleic acid is detected in the reaction vessel; (xii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the twelfth target nucleic acid is detected in the reaction vessel; (xiii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the thirteenth target nucleic acid is detected in the reaction vessel; (xiv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fifth color label or dye when the fourteenth target nucleic acid is detected in the reaction vessel; (xv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the sixth color label or dye when the fifteenth target nucleic acid is detected in the reaction vessel; (xvi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the sixteenth target nucleic acid is detected in the reaction vessel; (xvii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fifth color label or dye when the seventeenth target nucleic acid is detected in the reaction vessel; (xviii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the sixth color label or dye when the eighteenth target nucleic acid is detected in the reaction vessel; (xix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye when the nineteenth target nucleic acid is detected in the reaction vessel; 9Docket No. ABBTT-43596.601 (xx) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye when the twentieth target nucleic acid is detected in the reaction vessel; and (xxi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye when the thirtieth target nucleic acid is detected in the reaction vessel. In some embodiments of the above method, step (a) comprises (a) labeling at least seven different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least seven different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; and (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye, the second color label or dye, and the third color label or dye. In some embodiments of the above method, the digital multiplexed nucleic acid amplification assay comprises PCR or isothermal amplification. In another embodiment, the present disclosure relates to a method for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample. The method comprises the steps of: (a) labeling at least three different detection agents with at least two different color labels or dyes to create a set of color codes specific for at least three different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; 10Docket No. ABBTT-43596.601 (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; and (iii) a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) performing a digital immunoassay in parallel for each of the at least three different target antigens to detect, on average, a single one of the at least three different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; and (iii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the third detection agent detects the third target antigen. In some aspects of the above method, step (a) comprises labeling at least six different detection agents with at least three different colored labels or dyes to create a set of color codes specific for at least six different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first color and third color label or dyes; and (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye. In some aspects of the above method, step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least six different target antigens to detect, on average, a single one of the at least six different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; 11Docket No. ABBTT-43596.601 (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the fifth detection agent detects the fifth target antigen; and (vi) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the sixth detection agent detects the sixth target antigen. In some embodiments, step (a) comprises labeling at least 10 different detection agents with at least four different colored labels or dyes to create a set of color codes specific for at least 10 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first and second color labels or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the first and third color labels or dyes; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first and fourth color labels or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the second and third color labels or dyes; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the second and fourth color labels or dyes; and (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the third and fourth color label or dyes. In some embodiments, step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least 10 different target antigens to detect, on average, a single one of the at least 10 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye 12Docket No. ABBTT-43596.601 when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the eighth detection agent detects the eighth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the ninth detection agent detects the ninth target antigen; and (x) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the tenth detection agent detects the tenth target antigen. In yet another embodiment of the above method, step (a) comprises labeling at least 15 different detection agents with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a fifth color label or dye; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the first and second color labels or dyes; 13Docket No. ABBTT-43596.601 (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first and third color labels or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the first and fourth color labels or dyes; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the first and fifth color labels or dyes; (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the second color and third color labels or dyes; (xi) an eleventh detection agent specific for an eleventh target antigen is labeled with a combination of labels or dyes comprising the second and fourth color labels or dyes; (xii) a twelfth detection agent specific for a twelfth target antigen is labeled with a combination of labels or dyes comprising the second and the fifth color labels or dyes; (xiii) a thirteenth detection agent specific for a thirteenth target antigen is labeled with a combination of labels or dyes comprising the third color labels or dye and the fourth color label or dye; (xiv) a fourteenth detection agent specific for a fourteenth target antigen is labeled with a combination of labels or dyes comprising the third color labels or dye and the fifth color label or dye; and (xv) a fifteenth detection agent specific for a fifteenth target antigen is labeled with a combination of labels or dyes comprising the fourth color labels or dye and the fifth color label or dye. In yet another embodiment of the above method step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least 15 different target antigens to detect, on average, a single one of the at least 15 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the fifth color label or dye when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the 14Docket No. ABBTT-43596.601 first and second color labels or dyes when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the first and fifth color labels or dyes when the ninth detection agent detects the ninth target antigen; (x) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the tenth detection agent detects the tenth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the eleventh detection agent detects the eleventh target antigen; (xii) the digital immunoassay emits a light signal corresponding to the combination of the second and fifth color labels or dyes when the twelfth detection agent detects the twelfth target antigen; (xiii) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the thirteenth detection agent detects the thirteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the third and fifth color labels or dyes when the fourteenth detection agent detects the fourteenth target antigen; and (xv) the digital immunoassay emits a light signal corresponding to the combination of the fourth and fifth color labels or dyes when the fifteenth detection agent detects the fifteenth target antigen. In yet another embodiment of the above method, step (a) comprises labeling at least 21 different detection agents with at least six different colored labels or dyes to create a set of color codes specific for at least 21 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a fifth color label 15Docket No. ABBTT-43596.601 or dye; (vi) a sixth detection agent specific for a fifth target antigen is labeled with a sixth color label or dye; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (xi) an eleventh detection agent specific for an eleventh target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the sixth color label or dye; (xii) a twelfth detection agent specific for a twelfth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xiii) a thirteenth detection agent specific for a thirteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xiv) a fourteenth detection agent specific for a fourteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xv) a fifteenth detection agent specific for a fifteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the sixth color label or dye; (xvi) a sixteenth detection agent specific for a sixteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xvii) a seventeenth detection agent specific for a seventeenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; (xviii) an eighteenth detection agent specific for an eighteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the sixth color label or dye; (xix) a nineteenth detection agent specific for a nineteenth target antigen is labeled with a combination of labels and dyes comprising the fourth color label or dye and the fifth color label or dye; (xx) a twentieth detection agent specific for a twentieth target antigen is labeled with a 16Docket No. ABBTT-43596.601 combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye; and (xxi) a twenty-first detection agent specific for a twenty-first target antigen is labeled with a combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye. In yet another embodiment of the above method, step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least 21 different target antigens to detect, on average, a single one of the at least 21 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the fifth color label or dye when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the sixth color label or dye when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the ninth detection agent detects the ninth target antigen; (x) the digital immunoassay emits a light signal corresponding to the combination of the first and fifth color labels or dyes when the tenth detection agent detects the tenth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the first and sixth color labels or dyes when the eleventh detection agent detects the eleventh target antigen; (xii) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the twelfth detection agent detects the twelfth target antigen; (xiii) the digital immunoassay emits a light signal corresponding to the combination of the 17Docket No. ABBTT-43596.601 second and fourth color labels or dyes when the thirteenth detection agent detects the thirteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the second and fifth color labels or dyes when the fourteenth detection agent detects the fourteenth target antigen; (xv) the digital immunoassay emits a light signal corresponding to the combination of the second and sixth color labels or dyes when the fifteenth detection agent detects the fifteenth target antigen; (xvi) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the sixteenth detection agent detects the sixteenth target antigen; (xvii) the digital immunoassay emits a light signal corresponding to the combination of the third and fifth color labels or dyes when the seventeenth detection agent detects the seventeenth target antigen; (xviii) the digital immunoassay emits a light signal corresponding to the combination of the third and sixth color labels or dyes when the eighteenth detection agent detects the eighteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the fourth and fifth color labels or dyes when the nineteenth detection agent detects the nineteenth target antigen; (xx) the digital immunoassay emits a light signal corresponding to the combination of the fourth and sixth color labels or dyes when the twentieth detection agent detects the twentieth target antigen; and (xxi) the digital immunoassay emits a light signal corresponding to the combination of the fifth and sixth color labels or dyes when the twenty-first detection agent detects the twenty-first target antigen. In yet another embodiment of the above method, step (a) comprises: (a) labeling at least seven different detection agents with at least three different colored labels or dyes to create a set of color codes specific for at least seven different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; 18Docket No. ABBTT-43596.601 (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dyes; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first color and third color label or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the second and third color label or dyes; and (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first, second, and the third color label or dyes. In yet another embodiment, step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least seven different target antigens to detect, on average, a single one of the at least seven different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the sixth detection agent detects the sixth target antigen; and (vii) the digital immunoassay emits a light signal corresponding to the combination of the first, second, and third color labels or dyes when the seventh detection agent detects the seventh target antigen. In yet another embodiment of the above method, the digital multi-parallel immunoassay comprises a sandwich immunoassay. In yet another embodiment of the above method, each digital immunoassay is performed in a separate reaction vessel. In still yet another embodiment of the above method, the reaction vessel comprises a capture agent bound to a solid support in the reaction vessel. In still yet another embodiment of the above method, the reaction vessel comprises a capture agent bound to the reaction vessel. 19Docket No. ABBTT-43596.601 In still yet another embodiment, the present disclosure relates to a method for performing a digital multiplexed nucleic acid amplification assay to detect at least a first target nucleic acid in a sample. The method comprises the steps of: (a) labeling at least a first nucleic acid probe with at least two different colored labels or dyes to create a set of color codes specific for at least a first target nucleic acid of interest, wherein: (i) the first nucleic acid probe specific for the first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (b) aliquoting the sample into at least one reaction vessel, wherein on average, the at least one reaction vessel contains a single molecule of the target nucleic acid and the first nucleic acid probe; (c) amplifying in the at least one reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in the at least one reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when the first target nucleic acid is detected in the reaction vessel. In yet another embodiment, the present disclosure relates to a method for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample. The method comprises the steps of: (a) labeling at least a first detection agent with at least two different color labels or dyes to create a set of color codes specific for at least a first target antigens of interest, wherein: (i) the first detection agent specific for the first target antigen is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye, (b) performing a digital immunoassay in parallel for at least the first target antigen to detect, on average, a single one of at least the first target antigen of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen. In still yet another embodiment, the present disclosure relates to a kit for performing a digital multiplex nucleic acid amplification assay to detect a plurality of targets in a sample, wherein the kit comprises: (a) at least three different nucleic acid probes labeled with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein: 20Docket No. ABBTT-43596.601 (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) instructions for performing a digital multiplexed nucleic acid amplification assay, wherein the instructions instruct a user of the kit to: (i) aliquot the sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel (many will be empty) contains a single molecule of target nucleic acid and the at least three different nucleic acid probes; (ii) amplify in each reaction vessel, on average, the single molecule of the target nucleic acid; and (iii) detect in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (1) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (2) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (3) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel. In still yet another embodiment, the present disclosure relates to a kit for performing a digital multiplexed nucleic acid amplification assay to detect at least a first target nucleic acid in a sample, wherein the kit comprises: (a) at least a first nucleic acid probe labeled with at least two different colored labels or dyes to create a set of color codes specific for at least a first target nucleic acid of interest, wherein the first nucleic acid probe specific for the first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (b) instructions for performing a digital multiplexed nucleic acid amplification assay, wherein the instructions instruct a user of the kit to: (i) aliquot the sample into at least one reaction vessel, wherein on average, the at least one reaction vessel contains a single molecule of the target nucleic acid and the first nucleic acid probe; (ii) amplify in the at least one reaction vessel, on average, the single molecule of the target nucleic acid; and (iii) detect in the at least one reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein the reaction vessel emits a light signal 21Docket No. ABBTT-43596.601 corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when the first target nucleic acid is detected in the reaction vessel. In still yet another embodiment, the present disclosure relates to a kit for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, wherein the kit comprises: (a) at least a first detection agent labeled with at least two different color labels or dyes to create a set of color codes specific for at least a first target antigens of interest, wherein (i) the first detection agent specific for the first target antigen is labeled with a combination of dyes comprising at least a first color label or dye and at least a second color label or dye, (b) instructions for performing a digital multiplexed nucleic acid amplification assay, wherein the instructions instruct a user of the kit to: (i) perform a digital immunoassay in parallel for at least the first target antigen to detect, on average, a single one of at least the first target antigen of interest in each immunoassay, wherein the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen. In some aspects, one or more of the above kits further comprise one or more reaction vessels. In still further aspects, the digital multiplexed nucleic acid amplification assay performed using the above one or more kits comprises PCR or isothermal amplification. BRIEF DESCRIPTION OF THE DRAWINGS

[0001] The patent or application file contains drawings executed in color. Copies of this patent or patent application publication with color drawings will be provided by the Office upon request and payment of the necessary fee.

[0002] Having thus described the presently disclosed subject matter in general terms, reference will now be made to the accompanying Figures, which are not necessarily drawn to scale, and wherein:

[0003] FIG. 1A shows a diagram of a non-digital multiplex format indicating a sample (clear), a first target probe (yellow), a second target probe (blue), a mixture of the first and second target probes (green), and a mixture of three target probes (yellow, blue, and green), which are indistinguishable from the mixture of the first and second target probes. FIG.1B shows a diagram of a digital multiplex format indicating a well-plate of samples (clear), a well-plate of a first target probe (yellow), a well-plate of a second target probe (blue), a well-plate of a mixture of the first and second target probes (yellow and blue), and a well-plate of a mixture of three target probes (yellow, blue, and green), which are distinguishable from the mixture of the first and second target probes. FIG. 1C shows a digital multi- 22Docket No. ABBTT-43596.601 parallel format with fifteen spectral plots, illustrating the various combinations of multiplex use of four colored labels (green, orange, red, and blue) in one reaction vessel, given just four possible targets. FIG. 1D shows a digital multi-parallel format with six spectral plots, illustrating the quantitative differences in combination of colored labels (green, red and blue). DETAILED DESCRIPTION In an aspect, the present disclosure provides systems, methods and kits for performing a digital multiplexed nucleic acid amplification assay to detect a plurality of target nucleic acids in a sample, for example, using different colored labels or dyes to create a set of color codes specific for different target nucleic acids of interest in the sample. The systems, methods and kits of the this aspect of the disclosure involve: (a) labeling at least three different nucleic acid probes with at least two different color labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye, a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye, and a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) performing a digital amplification nucleic acid assay in a separate reaction vessel for each of the at least three different nucleic acid probes to amplify the at least three different target nucleic acids of interest; and (c) detecting the at least three different target nucleic acids of interest based on the color codes for each of the at least three different nucleic acids amplified in the sample. In another aspect, the present disclosure further provides systems, methods and kits for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, for example, using different colored labels or dyes to create a set of color codes specific for different target antigens of interest in the sample. The systems, methods and kits of this aspect of the disclosure involve: (a) labeling at least three different detection agents with at least two different color labels or dyes to create a set of color codes specific for at least three different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye, (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; and (iii) a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) performing a digital immunoassay in parallel for each of the at least three different target antigens to detect, on average, a single one of the at least three different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second 23Docket No. ABBTT-43596.601 target antigen; and (iii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the third detection agent detects the third target antigen. The work described herein involves the use of combinations of different color labels or dyes to label nucleic acid probes in nucleic acid assays or detection agents in immunoassays to expand the coding space for larger sets of targets. Typically, the number of targets that can be detected in a multiplex or multi-parallel detection scheme using labels or dyes is limited to the number of labels or dyes that can be detected independently in one reaction vessel. However, in a digital detection scheme the individual target molecules or target binding beads are separated into individual reaction vessel compartments. If only a set of individual labels or dyes are used for each target probe of interest, it is unlikely that multiple labels or dyes will be present in one reaction vessel. Thus in some aspects, this provides the opportunity to combine multiple labels or dyes onto one type of probe for additional targets. For instance, a red and blue label or dye can be used for two targets and then can be combined for a third target. Because the targets are spread out into separate reaction vessels, it will be possible to see red vessels where only target one is detected, blue vessels where only target two is present, and red and blue in vessels where only target three resides. Alternatively, in other aspects, a probe can be labeled with a first label or dye of one color, and the same probe can be labeled with a second label or dye of a second color, whose color is different than the first label or dye. In further aspects, the same probe can be labeled with a third label or dye of a third color, whose color is different than that used for the first and second labels or dyes. In still further aspects, the same probe can be labeled with a fourth label or dye of a fourth color, whose color is different than that used for the first-three labels or dyes. In yet further aspects, the same probe can be labeled with a fifth label or dye of a fifth color, whose color is different from that used for the first-fourth labels or dyes. In yet further aspects, the same probe can be labeled with a sixth label or dye of a sixth color, which color is different than that used for the first-fifth labels or dyes. Additional labels or dyes can be included to expand the coding space. It is practical to use at least six labels or dyes in PCR assays. If just two dye combination codes (50% of two labels or dyes per code) are used, there will be fourteen two-dye codes (5+4+3+2) plus the six individual labels or dyes for a total of a twenty-plex assay. It is also possible to use three dye codes to achieve many more codes, but there will become a limitation in the number of individual targets that can be detected due to the balance of the number of target types, number of reaction vessels, the dynamic range of the target types, and the likelihood that the targets will be represented just once per reaction vessel. Section headings as used in this section and the entire disclosure herein are merely for organizational purposes and are not intended to be limiting. 24Docket No. ABBTT-43596.601 1. Definitions Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In case of conflict, the present document, including definitions, will control. Preferred methods and materials are described below, although methods and materials similar or equivalent to those described herein can be used in practice or testing of the present disclosure. All publications, patent applications, patents and other references mentioned herein are incorporated by reference in their entirety. The materials, methods, and examples disclosed herein are illustrative only and not intended to be limiting. The terms “comprise(s),” “include(s),” “having,” “has,” “can,” “contain(s),” and variants thereof, as used herein, are intended to be open-ended transitional phrases, terms, or words that do not preclude the possibility of additional acts or structures. The singular forms “a,” “an” and “the” include plural references unless the context clearly dictates otherwise. The present disclosure also contemplates other embodiments “comprising,” “consisting of” and “consisting essentially of,” the embodiments or elements presented herein, whether explicitly set forth or not. For the recitation of numeric ranges herein, each intervening number there between with the same degree of precision is explicitly contemplated. For example, for the range of 6-9, the numbers 7 and 8 are contemplated in addition to 6 and 9, and for the range 6.0-7.0, the number 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, and 7.0 are explicitly contemplated. An “amount” as used herein refers to a quantity specified (e.g., high or low) or a number e.g., where the number is a level, such as a position on a real or imaginary scale of amount or quantity, or a concentration, such as, for example, a relative amount of a given substance contained within a solution or in a particular volume of space, e.g., the amount of solute per unit volume of solution. “Amplifying” or “amplification” in the context of nucleic acids refers to the production of multiple copies of a polynucleotide, or a portion of the polynucleotide, typically starting from a small amount of the polynucleotide (e.g., a single polynucleotide molecule), where the amplification products or amplicons are generally detectable. Amplification of polynucleotides encompasses a variety of chemical and enzymatic processes. Generation of multiple DNA copies from one or a few copies of a target or template DNA molecule during a polymerase chain reaction (PCR), isothermal reaction, or a ligase chain reaction (LCR) are forms of amplification. Amplification is not limited to the strict duplication of the starting molecule. For example, the generation of multiple cDNA molecules from a limited amount of RNA in a sample using reverse transcription (RT)-PCR is a form of amplification. Furthermore, the generation of multiple RNA molecules from a single DNA molecule during the process of transcription is also a form of amplification. An “analog assay” as used herein refers to an assay in which the presence of and / or amount of an analyte in a test sample is determined by measuring the total signal produced (e.g., fluorescence, 25Docket No. ABBTT-43596.601 color, etc.) by the analyte in an entire reaction mixture (e.g., in a reaction vessel). In an analog assay, the noise is indistinguishable from the signal. An example of an analog assay is an assay in which the presence of and / or amount of an analyte is determined by measuring the total signal produced from a plurality of beads or microparticles contained in a reaction vessel. “Analyte”, “analyte of interest”, “biomarker” or “biomarker of interest” as used interchangeably herein, refers to a naturally occurring or synthetic biological molecule found in an organism, in which the varying concentrations provide information useful in predicting the risk, occurrence, or severity of a disease or condition. In some embodiments, the analyte is a biomolecule. Non-limiting examples of biomolecules include macromolecules such as proteins (e.g., peptides, polypeptides, protein fragments, protein complexes, fusion proteins, recombinant proteins, phosphoproteins, glycoproteins, lipoproteins), lipids (e.g., fatty acids, cholesterol, triglycerides, phospholipids), or carbohydrates (e.g., glucose, glycogen, glycoproteins, glycolipids). In certain instances, the analyte may be hormones (e.g., insulin, cortisol, estrogen, testosterone), antibodies, growth factors (e.g., epidermal growth factor, insulin-like growth factor), cytokines (e.g., interleukins, tumor necrosis factor, interferons), enzymes (e.g., alanine transaminase, creatine kinase), receptors (e.g., neural receptors, hormonal receptors, nutrient receptors, cell surface receptors) and their ligands, cancer markers (e.g., prostate-specific antigen (PSA), tumor necrosis factor-alpha (TNF-alpha)), markers of myocardial infarction (e.g., troponin, creatine kinase), toxins (e.g., heavy metals, environmental pollutants), drugs (e.g., drugs of addiction, pharmaceuticals), metabolic agents (e.g., vitamins, minerals), non-naturally occurring polymorphisms (e.g., genetic variations, mutations) and the like. In some embodiments, the analyte may be a post-translationally modified protein (e.g., phosphorylated, methylated, glycosylated protein) and the first or the second binding member may be an antibody specific to a post-translational modification. A modified protein may be bound to a first binding member immobilized on a solid support where the first binding member binds to the modified protein but not the unmodified protein. In other embodiments, the first binding member may bind to both the unmodified and the modified protein, and the second binding member may be specific to the post-translationally modified protein. In yet other embodiments, the analyte may be a cell, such as, a circulating tumor cell, stem cells, white blood cells (leukocytes), red blood cells (erythrocytes), platelets (thrombocytes), immune cells (e.g., lymphocytes, macrophages, neutrophils), endothelial cells, epithelial cells (e.g., from the skin, urinary tract, gastrointestinal tract), mesenchymal cells, neuronal cells, pathogenic bacteria, viruses (including retroviruses, herpesviruses, adenoviruses, lentiviruses, Filoviruses (ebola), hepatitis viruses (e.g., A, B, C, D, and E, including surface antigens, core antigens, etc.), human immunodeficiency viruses (e.g., HIV-1, HIV-2), paroviruses, Esptein-Barr Virus, HPV), parasites (e.g., babesia, Trypanosoma cruzi), spores, etc. 26Docket No. ABBTT-43596.601 A non-limiting list of analytes include: Anti-Mullerian Hormone (AMH), Autoantibody to CD25, CXCL13, Dkk-3 (Semen), IL-12p40, IL-8, p14 Endocan Fragment, SARS-CoV-2 IgA Antibody, SARS-CoV-2 IgG Antibody, SARS-CoV-2 IgM Antibody, Secretory Gelsolin (pGSN), Secretogranin II, ACE2, Albumin, Albuminuria, Alpha-Amylase, Apo H, B-2 Microglobulin Levels, CA 24-2, Carcinoembryonic Antigen (CEA), Ceruloplasmin, Chloride, Cholinesterase, Copper, CRP (C-Reactive Protein), Fibrinogen Alpha Chain (FGA), Homocysteine, IL-18 (Interleukin 18), IL-6 (Interleukin 6), LD (Lactate Dehydrogenase), L-FABP (Liver Fatty Acid Binding Protein), Lipase, Microalbuminuria, Neutrophil Gelatinase-Associated Lipocalin (NGAL), Osteopontin, PGC-1a (Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha), PKR (Proapoptotic Kinase R) and its phosphorylated PKR (pPKR), PCT (Procalcitonin), Pepsinogen I, Pepsinogen II, Pro-SFTPB, PTH (Parathyroid Hormone), sIL-2, SHBG (Sex Hormone-Binding Globulin), Thioredoxin, TNFα (Tumor Necrosis Factor-Alpha), TSH (Thyroid Stimulating Hormone), Vitamin D-Binding Protein, Alpha-synuclein, BARF1 (BamH1-A Reading Frame 1), Kidney Injury Molecule- 1 (KIM-1), Laminin gamma (e.g., laminin gamma subunit 1, laminin gamma subunit 2, laminin gamma subunit 3), LMP1 (Latent Membrane Protein 1), Neurofilament light chain, Tau protein, UCH-L1 (Ubiquitin C-Terminal Hydrolase-L1), Alkaline Phosphatase, Amylase, AST (Aspartate Aminotransferase), Calcium, Cholesterol, CK (Creatine Kinase), CO2 (Carbon Dioxide), Creatinine, Direct LDL (Direct Low-Density Lipoprotein), GGT (Gamma-Glutamyl Transferase), HDL (High- Density Lipoprotein), Iron, LDL (Low-Density Lipoprotein), Magnesium, Potassium (K), Sodium (Na), Triglycerides, Uric Acid, Akt (Protein Kinase B), Amphiregulin, ANXA7 (Annexin A7), AR (Androgen Receptor), BRAF (v-Raf Murine Sarcoma Viral Oncogene Homolog B), CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B), cMYC (MYC Proto-Oncogene), CTNNB1 (Catenin Beta-1), EGFR (Epidermal Growth Factor Receptor), EPHB2 (Ephrin Type-B Receptor 2), ESR1 (Estrogen Receptor 1), ESR2 (Estrogen Receptor 2), FOXO3A (Forkhead Box O3), FRAP1 (Mechanistic Target of Rapamycin Complex 1), FRS2 (Fibroblast Growth Factor Receptor Substrate 2), Gab2 (GRB2- Associated Binding Protein 2), GFAP (Glial Fibrillary Acidic Protein), Grb2 (Growth Factor Receptor-Bound Protein 2), GREB1 (Growth Regulation By Estrogen In Breast Cancer 1), HER2 (Human Epidermal Growth Factor Receptor 2), HER3 (Human Epidermal Growth Factor Receptor 3), HER4 (Human Epidermal Growth Factor Receptor 4), IGF-IR (Insulin-Like Growth Factor 1 Receptor), IL6R (Interleukin-6 Receptor), KLF6 (Kruppel Like Factor 6), KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog), LZTS1 (Leucine Zipper Tumor Suppressor 1), MAP2K1 (Mitogen-Activated Protein Kinase Kinase 1), MEK (Mitogen-Activated Protein Kinase Kinase), MIG-6 (Mitogen-Inducible Gene 6 Protein), MKI67 (Marker of Proliferation Ki-67), mTOR (Mechanistic Target of Rapamycin), MUC4 (Mucin 4, Cell Surface Associated), NEDD4-1 (Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 4-1), NKX3-1 (NK3 Homeobox 1), NRG1 (Neuregulin 1), Parkin, PDK-1 (3-Phosphoinositide-Dependent Protein Kinase-1), PGR (Progesterone Receptor), PHLPP (PH Domain and Leucine-Rich Repeat Protein Phosphatase), 27Docket No. ABBTT-43596.601 PIK3CA (Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha), PITX2 (Paired- Like Homeodomain Transcription Factor 2), PPP1R1B (Protein Phosphatase 1 Regulatory Inhibitor Subunit 1B), PRDX6 (Peroxiredoxin-6), PTEN, PTEN 1 (Phosphatase and Tensin Homolog 1), PXN (Paxillin), S6K (Ribosomal Protein S6 Kinase), SHIP (Src Homology 2 Domain-Containing Inositol Phosphatase), Src (Proto-Oncogene Tyrosine-Protein Kinase Src), TOPO II (DNA Topoisomerase II), TSC1 (Tuberous Sclerosis 1), TSC2 (Tuberous Sclerosis 2), Tumor necrosis factor-alpha receptors, VEGF (Vascular Endothelial Growth Factor), Aβ42 (Amyloid Beta-Protein 42), CK-MB (Creatine Kinase-MB), Anti-CCP (Anti-Cyclic Citrullinated Peptide), Anti-Tg (Anti-Thyroglobulin Antibody), Anti-TPO (Anti-Thyroid Peroxidase Antibody), ASO (Antistreptolysin O), C3 (Complement Component 3), C4 (Complement Component 4), D-Dimer, HIV Ag / Ab Combo (Human Immunodeficiency Virus Antigen / Antibody Combination), RF (Rheumatoid Factor), DJ-1, Leucine- rich repeat kinase 2, Mutated ATP13A2, Prion protein, PTEN induced putative kinase 1, Troponin (such as troponin I, troponin T , macrotropnin and / or troponin C), AFP (Alpha-Fetoprotein), CA 125 (MUC16), CA 15-3, CA 19-9, CCNI (Cyclin I), CMV (Cytomegalovirus), CYFRA21-1, EGRF (Epidermal Growth Factor Receptor), FGF19 (Fibroblast Growth Factor 19), HE-4 (Human Epididymis Protein 4), NSE (Neuron-Specific Enolase), p-ANCA (Perinuclear Anti-Neutrophil Cytoplasmic Antibody), PIVKA, PIVKA-II (Protein Induced By Vitamin K Absence-II), Pro-SFTPB, PSA (Prostate Specific Antigen), Rubella, SCC (Squamous Cell Carcinoma Antigen), Toxo IgG, Toxo IgM, Beta-hcG (Beta Human Chorionic Gonadotropin), Botulinum toxins, Clostridium difficile toxins A and B, DHEA-S (Dehydroepiandrosterone Sulfate), Diphtheria toxin, E. coli enterotoxins (heat-labile exotoxin, heat-stable enterotoxin), Estradiol, Fetuin-A, FSH (Follicle-Stimulating Hormone), Glycosylated hemoglobin (HbA1c), Hemoglobin A1c, IL1α (Interleukin 1 alpha), Influenza HA antigen, Insulin, LH (Luteinizing Hormone), Myeloperoxidase (MPO), NF-1 (Neurofibromin 1), Plasma C-peptide, PlGF (Placental Growth Factor), Pro-GFP, Prolactin, S100β, sFlt-1 (Soluble Fms-Like Tyrosine Kinase-1), Testosterone, Tetanus toxin, Thymosin 1315, ALT (Alanine Aminotransferase), Bile Acids, Total, Bilirubin, Bilirubin, Direct, Bilirubin, Total, Calprotectin, DUTPase (Deoxyuridine Triphosphatase), Glucose, Lactic Acid, Lactoferrin, Protein, Shiga toxin, Shiga-like toxin I, Shiga-like toxin II, Total Protein, Urea Nitrogen, VDAC1 (Voltage- Dependent Anion Channel 1), Wilm's Tumor-1 protein, Amphetamine / Methamphetamine, Barbiturates, Benzodiazepines, Benzodiazepines (Serum), Cannabinoids, Cocaine, Ecstasy, Ethanol, Methadone, Opiates, PCP (Phencyclidine), propoxyphene, salicylate, or tricyclic antidepressants. In another aspect, the analyte is cardiac myosin binding protein C, myoglobin, brain natriuretic peptide and derivatives thereof (e.g., preproBNP, proBNP, NT-proBNP, BNP). In still other aspects, the analyte is digoxin, gentamicin, methotrexate, phenobarbital, phenytoin, theophylline, valproic acid, vancomycin, B12, C-peptide, cortisol, ferritin, folate, insulin, vitamin D folate RBC, Free T3, Free T4, Thyroglobulin, Total T3, Total T4, TRAb, cyclosporine, sirolimus, tacrolimus, DDP4, or periostin. 28Docket No. ABBTT-43596.601 Examples of nucleic acid aptamers include: drugs of abuse (e.g. cocaine), protein analytes (including, but not limited to, Nucleolin, nuclear factor-kB essential modulator (NEMO), CD-30, protein tyrosine kinase 7 (PTK7), vascular endothelial growth factor (VEGF), MUC1 glycoform, immunoglobulin µ Heavy Chains (IGHM), Immunoglobulin E, αvβ3 integrin, α-thrombin, NF-κB, E2F transcription factor, HER3, Plasminogen activator inhibitor, Tenascin C, CXCL12 / SDF-1, prostate specific membrane antigen (PSMA), gastric cancer cells, HGC-27); cells (including, but not limited to, non-small cell lung cancer (NSCLC), colorectal cancer cells, (DLD-1), H23 lung adenocarcinoma cells, Ramos cells, T-cell acute lymphoblastic leukemia (T-ALL) cells, CCRF-CEM, acute myeloid leukemia (AML) cells (HL60), small-cell lung cancer (SCLC) cells, NCIH69, human glioblastoma cells, U118-MG, PC-3 cells, HER-2-overexpressing human breast cancer cells, SK-BR- 3, pancreatic cancer cell line (Mia-PaCa-2)); and infectious agents (including, but not limited to, Mycobacterium tuberculosis, Staphylococcus aureus, Shigella dysenteriae, Escherichia coli O157:H7, Campylobacter jejuni, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella 08, Salmonella enteritidis). Examples of protein or peptide aptamers include: HBV core capsid protein, CDK2, E2F transcription factor, Thymidylate synthase, Ras, EB1, and Receptor for Advanced Glycated End products (RAGE). Aptamers, and use and methods of production thereof are reviewed in e.g., Shum et al., J Cancer Ther.20134:872; Zhang et al., Curr Med Chem.2011; 18:4185; Zhu et al., Chem Commun (Camb). 201248:10472; Crawford et al., Brief Funct Genomic Proteomic.20032:72; Reverdatto et al., PLoS One 20138:e65180. “Bead,” “microparticle,” and “particle” are used herein interchangeably and refer to a substantially spherical solid support. Microparticles that can be used herein can be any type known in the art. For example, the bead or microparticle can be a magnetic bead or magnetic particle. Magnetic beads / particles may be ferromagnetic, ferrimagnetic, paramagnetic, superparamagnetic or ferrofluidic. Exemplary ferromagnetic materials include Fe, Co, Ni, Gd, Dy, CrO2, MnAs, MnBi, EuO, and NiO / Fe. Examples of ferrimagnetic materials include NiFe2O4, CoFe2O4, Fe3O4(or FeO.Fe2O3). Beads can have a solid core portion that is magnetic and is surrounded by one or more non-magnetic layers. The size of the microparticles used in the systems, methods and kits described herein can vary. Preferably, the microparticles have a substantially uniform diameter of less than about 0.10 µm, less than about 0.20 µm, less than about 0.30 µm, less than about 0.40 µm, less than about 0.50 µm, less than about 0.60 µm, less than about 0.70 µm, less than about 0.80 µm, less than about 0.9 µm, less than about 1 µm, less than about 2 µm, less than about 3 µm, less than about 4 µm, or less than about 5 µm. The skilled artisan will appreciate, however, that the microparticles should be sized to support diffusion of the microparticles in solution. In some instances, for example, for larger sized 29Docket No. ABBTT-43596.601 microparticles, the disclosure contemplates agitation of the sample (e.g., aliquot) to ensure that the microparticles are sufficiently dispersed throughout the sample. “Capillary blood sample” as used herein refers to a sample of blood from the capillaries which is obtained (e.g., extracted) through the skin (and not the veins) of a subject using a syringe, needle, or any other suitable device or combination thereof. For example, a whole blood sample can be obtained from the skin on the fingers and / or toes, a hand, a foot (including the heel), an earlobe, a location on the arms and / or legs, chest, back, head, or any combinations thereof. In some embodiments, the capillary blood sample is whole blood, serum or plasma. In yet other embodiments, the capillary blood sample contains predominantly capillary blood, but may also contain or comprise a small amount or percentage of interstitial fluid. “Cartridge” as used herein refers to a hollow container and / or chip that comprises one or more substances and / or components (e.g., a liquid, reagents (e.g., antibodies and / or antigens), and / or a particle (e.g., a bead, or microparticle)) for insertion into an apparatus (e.g., a point-of-care device). In some aspects, a cartridge has one or more apertures. In some aspects, a cartridge is a microfluidic cartridge. “Communicating the amount” as used herein, refers to communicating, as described herein, the amount, in terms of presence (i.e., a qualitative measure), or in terms of a level or levels (i.e., a quantitative measure), such that “amount” refers to presence or level. In some aspects, “communicating the amount” refers to communicating the presence (e.g., qualitative measure) of an analyte or biomarker. In other aspects, “communicating the amount” refers to communicating the level (e.g., quantitative measure) of an analyte or biomarker. "Colorimetric" refers to the physical description and quantification of chromatography, including human color perception spectroscopy (e.g., visible spectroscopy). In some embodiments, colorimetric assays are particularly useful when quantification is not required and there are no expensive detection devices. In certain embodiments, detection of a color change may be performed by visual inspection by a user (e.g., the person performing the assay). Because the colorimetric assay can be detected by visual inspection, the user can check for a detectable change in the color of the reaction, or the assay can be run in parallel with one or more controls (positive or negative) that replicate the color of the comparable reaction. In some embodiments, calibrated colorimetric measurements may be used to quantify the amount of the target. Typically, colorimetric analysis involves determining the presence / absence, level or concentration of an analyte (e.g., a chemical element or compound) in a sample (e.g., a solution) with the aid of a color-developer. It is suitable for organic and inorganic compounds and can be used with or without an enzymatic reaction step. Typically, the desired device is a colorimeter, one or more cuvettes and a suitable color developer. The process may be automated, for example by using an automated analyzer or by flow injection 30Docket No. ABBTT-43596.601 analysis. In particular embodiments, the colorimeter may be adapted for use with a plate reader to accelerate analysis and reduce waste streams. “Component,” “components,” or “at least one component,” refer generally to a specific binding member (e.g., a capture agent, e.g., capture antibody or aptamer), a detection or conjugate a calibrator, a control, a sensitivity panel, a container, a buffer, a diluent, a salt, an enzyme, a co-factor for an enzyme, a detection agent, a pretreatment reagent / solution, a substrate (e.g., as a solution), a stop solution, and the like that can be included in a kit for assay of a test sample, such as a patient whole blood, serum or plasma sample, in accordance with the methods described herein and other methods known in the art. Some components can be in solution or lyophilized for reconstitution for use in an assay. "Decentralize”, “Decentralized”, or “Decentralization”, as used interchangeably herein, refers to, in the context of testing, the performance of one or more medical tests and / or assays outside of a traditional medical setting (e.g., a hospital, physician office, stand alone lab site, etc.) to one or more places such as urgent care clinics, retail clinics, pharmacies, grocery stores or convenience stores, residences (e.g., homes, apartments, etc.), workplaces, and / or government offices (e.g., U.S. Transportation and Safety Authority), etc. “Hybrid-decentralization” or “hybrid-decentralized” refers to situations in which a subject or patient collects a sample at a residence and / or workplace and ships the sample to a laboratory, avoiding a professional collection site (such as a hospital, physician’s office, or stand-alone sample collection or lab site). “Determined by an assay” is used herein to refer to the determination of a reference level by any appropriate assay. The determination of a reference level may, in some embodiments, be achieved by an assay of the same type as the assay that is to be applied to the sample from the subject (for example, by an immunoassay, clinical chemistry assay, a single molecule detection assay, protein immunoprecipitation, immunoelectrophoresis, chemical analysis, SDS-PAGE and Western blot analysis, or protein immunostaining, electrophoresis analysis, a protein assay, a competitive binding assay, a functional protein assay, or chromatography or spectrometry methods, such as high- performance liquid chromatography (HPLC) or liquid chromatography–mass spectrometry (LC / MS)). The determination of a reference level may, in some embodiments, be achieved by an assay of the same type and under the same assay conditions as the assay that is to be applied to the sample from the subject. As noted herein, this disclosure provides exemplary reference levels (e.g., calculated by comparing reference levels at different time points). It is well within the ordinary skill of one in the art to adapt the disclosure herein for other assays to obtain assay-specific reference levels for those other assays based on the description provided by this disclosure. For example, a set of training samples comprising samples obtained from human subjects known to have a disease or condition and samples obtained from human subjects known not to have the disease or condition may be used to obtain assay-specific reference levels. It will be understood that a reference level “determined by an assay” 31Docket No. ABBTT-43596.601 and having a recited level of “sensitivity” and / or “specificity” is used herein to refer to a reference level which has been determined to provide a method of the recited sensitivity and / or specificity when said reference level is adopted in the methods of the disclosure. It is well within the ordinary skill of one in the art to determine the sensitivity and specificity associated with a given reference level in the methods of the disclosure, for example by repeated statistical analysis of assay data using a plurality of different possible reference levels. “Digital assay” as used herein refers to an assay in which an analyte is bound or captured and a molecule of the analyte segregated and interrogated (e.g., to detect the presence and / or amount of the analyte in a sample). In a digital assay, noise is separated from signal. In a digital assay, the results are assigned a value of 1 or 0. Examples of digital assays include one or more of the following (which may overlap but are not mutually exclusive): multiplexed nucleic acid amplification (e.g., digital PCR or isothermal amplification), single molecule detection assay, a nanowell assay, a multiplexed immunoassay (e.g., sandwich immunoassay), a single molecule enzyme linked immunosorbent assay, a direct capture counting assay, etc. As used herein, “digital microfluidics (DMF),” “digital microfluidic module (DMF module),” or “digital microfluidic device (DMF device)” as used interchangeably herein refer to a module, device, or system (e.g., micro-total analysis systems, or biomedical (or biological) microelectromechanical systems (BIOMEMs)) that utilizes digital or droplet-based microfluidic techniques to provide for manipulation of discrete and small volumes of liquids in the form of droplets. Digital microfluidics uses the principles of emulsion science to create fluid-fluid dispersion into channels (principally water-in-oil emulsion). It allows the production of monodisperse drops / bubbles or with a very low polydispersity. Digital microfluidics is based upon the micromanipulation of discontinuous fluid droplets within a reconfigurable network. Complex instructions can be programmed by combining the basic operations of droplet formation, translocation, splitting, and merging. Digital microfluidics operates on discrete volumes of fluids that can be manipulated by binary electrical signals. By using discrete unit-volume droplets, a microfluidic operation may be defined as a set of repeated basic operations, i.e., moving one unit of fluid over one unit of distance. Droplets may be formed using surface tension properties of the liquid. Actuation of a droplet is based on the presence of electrostatic forces generated by electrodes placed beneath the bottom surface on which the droplet is located. Different types of electrostatic forces can be used to control the shape and motion of the droplets. One technique that can be used to create the foregoing electrostatic forces is based on dielectrophoresis which relies on the difference of electrical permittivities between the droplet and surrounding medium and may utilize high-frequency AC electric fields. Another technique that can be used to create the foregoing electrostatic forces is based on electrowetting, which relies on the dependence of surface tension between a liquid droplet present on a surface and 32Docket No. ABBTT-43596.601 the surface on the electric field applied to the surface. Digital microfluidics can be performed a cartridge a chip (e.g., lab-on-chip, biochip), or a combination thereof. As used herein, the term “digital PCR” (dPCR) refers to a nucleic acid amplification method for the quantitation and analysis of target nucleic acid sequences by partitioning the reaction into a sufficient number of reaction subvolumes so that the target is in limiting dilution, i.e., producing a sufficient number of reaction subvolumes with zero target copies so as to allow the application of Poisson statistics. As used herein, “digital PCR methods” refer to the use of one or more of the steps typically employed by dPCR, but does not require that the “digital assumption” be achieved, namely, the assumption that any reaction subvolume that produced target amplicons had a single initial copy of the target sequence. As used herein, the term “droplet” refers to a small volume of liquid that is immiscible with its surroundings (e.g. gases, liquids, surfaces, etc.). A droplet may reside upon a surface, be encapsulated by a fluid with which it is immiscible (e.g. the continuous phase of an emulsion, a gas (e.g. air, nitrogen)), or a combination thereof. A droplet is typically spherical or substantially spherical in shape, but may be non-spherical. The shape of an otherwise spherical or substantially spherical droplet may be altered by deposition onto a surface or constriction in a capillary channel of smaller diameter. A droplet may be a “simple droplet” or a “compound droplet,” wherein one droplet encapsulates one or more additional smaller droplets. The volume of a droplet and / or the average volume of a set of droplets provided herein is typically less than about one microliter (e.g.0.1 μL ... 10 nL ...1 nL ...0.1 nL ...10 μL ...1 μL ...0.1 μL ...100 fL ...10 fL ...1 fL). The diameter of a droplet and / or the average diameter of a set of droplets provided herein is typically less than about one millimeter (e.g.1 mm ...100 μm ...10 μm ...1 μm). Droplets may be formed by any suitable technique (e.g. emulsification, microfluidics, injection etc.) and may be monodisperse (e.g., substantially monodisperse) or polydisperse. “Dynamic range” as used herein refers to range over which an assay readout is proportional to the amount of target molecule or analyte in the sample being analyzed. “Higher throughput assay analyzer” or a “non-point-of-care device”, as used interchangeably herein, refers to a device that is not a point-of-care device or a single use device. A higher throughput assay analyzer or non-point-of-care device refers to any device that does not meet any of the limitations of a point-of-care or a single use device as defined herein. In some embodiments, a “higher throughput assay analyzer” or “non-point-of-care device” refers to an instrument that: (a) may be a relatively large instrument compared to a hand-held point-of-care device, e.g., such as ranging in size from that of a tabletop instrument (e.g., typically considered low- or medium-throughput) to a large room-size or multiple-room-size instrument (e.g., typically considered high throughput); (b) is not a handheld instrument; (c) is capable of performing an assay 33Docket No. ABBTT-43596.601 on more than one clinical sample simultaneously; and (d) any combination of (a)-(c). A higher throughput assay analyzer may be a clinical chemistry analyzer, an immunoassay analyzer, or a combination thereof. Exemplary higher throughput assay analyzers or non-point-of-care devices include, for example, the ARCHITECT or Alinity platforms produced by Abbott Laboratories. As used herein the term “hydrophilic”, such as in reference to a “hydrophilic material” (e.g., membrane, microparticle, film, etc.) refers to those materials having a water contact angle of less than about 40 degrees. As used herein the term “hydrophobic”, such as in reference to a “hydrophobic material” (e.g., membrane, microparticle, film, etc.) refers to those materials having a water contact angle greater than about 80 degrees. “Label” and “detectable label” as used interchangeably herein refer to a moiety attached to an antibody or an analyte to render the reaction between the antibody and the analyte detectable, and the antibody or analyte so labeled is referred to as “detectably labeled.” A label can produce a signal that is detectable by visual or instrumental means. Various labels include signal-producing substances, such as chromagens, colorimetric reagents, fluorescent compounds, chemiluminescent compounds, radioactive compounds, and the like. Representative examples of labels include moieties that produce light, e.g., acridinium compounds, and moieties that produce fluorescence, e.g., fluorescein. Other labels are described herein. In this regard, the moiety itself may not be detectable but may become detectable upon reaction with yet another moiety. Use of the term “detectably labeled” is intended to encompass such labeling. Any suitable detectable label, as is known in the art, can be used. For example, the detectable label can be a radioactive label (such as 3H, 14C, 32P, 33P, 35S, 90Y, 99Tc, 111In, 125I, 131I, 177Lu, 166Ho, and 153Sm), an enzymatic label (such as horseradish peroxidase (substrate includes 3,3',5,5'-Tetramethylbenzidine (TMB)), alkaline peroxidase (substrate includes pNPP (p- Nitrophenyl phosphate), alkaline phosphatase (substrates include pNPP (p-Nitrophenyl phosphate), naphthol AS-BI-phosphate, and BCIP / NBT (5-Bromo-4-chloro-3-indolyl phosphate / Nitro blue tetrazolium)) glucose 6-phosphate dehydrogenase (substate includes D-glucose-6-phosphate), and the like), a chemiluminescent label (such as acridinium esters, thioesters, or sulfonamides; luminol, isoluminol, phenanthridinium esters, and the like), a fluorescent label (such as fluorescein (e.g., 5- fluorescein, 6-carboxyfluorescein, 3’6-carboxyfluorescein, 5(6)-carboxyfluorescein, 6-hexachloro- fluorescein, 6-tetrachlorofluorescein, fluorescein isothiocyanate, and the like)), rhodamine, phycobiliproteins, R-phycoerythrin, quantum dots (e.g., zinc sulfide-capped cadmium selenide), a thermometric label, or an immuno-polymerase chain reaction label. An introduction to labels, labeling procedures and detection of labels is found in Polak and Van Noorden, Introduction to Immunocytochemistry, 2nd ed., Springer Verlag, N.Y. (1997), and in Haugland, Handbook of Fluorescent Probes and Research Chemicals (1996), which is a combined handbook and catalogue 34Docket No. ABBTT-43596.601 published by Molecular Probes, Inc., Eugene, Oregon. A fluorescent label can be used in FPIA (see, e.g., U.S. Patent Nos.5,593,896, 5,573,904, 5,496,925, 5,359,093, and 5,352,803, which are hereby incorporated by reference in their entireties). An acridinium compound can be used as a detectable label in a homogeneous chemiluminescent assay (see, e.g., Adamczyk et al., Bioorg. Med. Chem. Lett. 16: 1324-1328 (2006); Adamczyk et al., Bioorg. Med. Chem. Lett.4: 2313-2317 (2004); Adamczyk et al., Biorg. Med. Chem. Lett.14: 3917-3921 (2004); and Adamczyk et al., Org. Lett.5: 3779-3782 (2003)). Other labels that can be used include a tag attached to a specific binding member or analyte by a cleavable linker. In one embodiment, the acridinium compound is an acridinium-9-carboxamide. Methods for preparing acridinium 9-carboxamides are described in Mattingly, J. Biolumin. Chemilumin.6: 107-114 (1991); Adamczyk et al., J. Org. Chem.63: 5636-5639 (1998); Adamczyk et al., Tetrahedron 55: 10899-10914 (1999); Adamczyk et al., Org. Lett.1: 779-781 (1999); Adamczyk et al., Bioconjugate Chem.11: 714-724 (2000); Mattingly et al., In Luminescence Biotechnology: Instruments and Applications; Dyke, K. V. Ed.; CRC Press: Boca Raton, pp.77–105 (2002); Adamczyk et al., Org. Lett.5: 3779-3782 (2003); and U.S. Patent Nos.5,468,646, 5,543,524 and 5,783,699 (each of which is incorporated herein by reference in its entirety for its teachings regarding same). Another example of an acridinium compound is an acridinium-9-carboxylate aryl ester. An example of an acridinium-9-carboxylate aryl ester of formula II is 10-methyl-9- (phenoxycarbonyl)acridinium fluorosulfonate (available from Cayman Chemical, Ann Arbor, MI). Methods for preparing acridinium 9-carboxylate aryl esters are described in McCapra et al., Photochem. Photobiol., 4: 1111-21 (1965); Razavi et al., Luminescence 15: 245-249 (2000); Razavi et al., Luminescence 15: 239-244 (2000); and U.S. Patent No.5,241,070 (each of which is incorporated herein by reference in its entirety for its teachings regarding same). Such acridinium-9-carboxylate aryl esters are efficient chemiluminescent indicators for hydrogen peroxide produced in the oxidation of an analyte by at least one oxidase in terms of the intensity of the signal and / or the rapidity of the signal. The course of the chemiluminescent emission for the acridinium-9-carboxylate aryl ester is completed rapidly, i.e., in under 1 second, while the acridinium-9-carboxamide chemiluminescent emission extends over 2 seconds. Acridinium-9-carboxylate aryl ester, however, loses its chemiluminescent properties in the presence of protein. Therefore, its use requires the absence of protein during signal generation and detection. Methods for separating or removing proteins in the sample are well-known to those skilled in the art and include, but are not limited to, ultrafiltration, extraction, precipitation, dialysis, chromatography, and / or digestion (see, e.g., Wells, High Throughput Bioanalytical Sample Preparation. Methods and Automation Strategies, Elsevier (2003)). The amount of protein removed or separated from the test sample can be about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, or about 95%. Further details regarding acridinium-9-carboxylate aryl ester and its use are set 35Docket No. ABBTT-43596.601 forth in U.S. Patent App. No.11 / 697,835, filed April 9, 2007. Acridinium-9-carboxylate aryl esters can be dissolved in any suitable solvent, such as degassed anhydrous N,N-dimethylformamide (DMF) or aqueous sodium cholate. “Microsampling device” as used herein refers to any device known in the art that is suitable for extracting capillary blood through the skin. It is understood that while a sample obtained through the skin using a microsampling device will comprise predominantly capillary blood, the sample may also comprise a small amount or percentage of interstitial fluid. In some aspects, the microsampling device can comprise from about 0.1 mL to about 4 mL of capillary blood. In some other aspects, the device contains a plurality of microneedles, lancets or microlancets, blades or microblades, microscrews, or any combination thereof. In some aspects, the plurality of microneedles, lancets or microlancets, blades or microblades, microscrews, or any combination thereof can be rotating. In yet other aspects, the plurality of microneedles, lancets or microlancets, blades or microblades, microscrews, or any combination thereof are non-rotating. In some aspects, the microsampling device creates a vacuum and / or uses a stored vacuum to pull the skin into the device and / or activate the plurality of microneedles, lancets or microlancets, blades or microblades, microscrews, or any combination thereof to cut the skin. Examplary microsampling devices which can be used in the methods described herein include the TAP device available from YourBio Health, Inc. (Cambridge, MA) as well as the device described in U.S. Patent No.9,113,836, the contents of which are herein incorporated by reference, the Tasso+, Tasso-M20, and Tasso-ST devices available from Tasso, Inc. (Seattle, WA), the One Draw device available from Draw Bridge Health (San Diego, CA), PBS-1000 from PreciHealth (Neuchatel, Switzerland) or the Loop blood collection device available from Loop Medical (Lausanne, Switzerland). In other aspects, an example of a microsampling device includes a fingerstick device. In some aspects, the microsampling device can include a band-aid, bandage, or other suitable material which can be applied or dispensed to the area of the skin once the sample is obtained and / or the device is removed and / or detached from the skin. “Normalize” Normalize” or “normalizing” as used herein refers adjusting the amount of an analyte determined in a capillary blood sample obtained from a subject based on the amount of the same analyte in venous blood. In some aspects, for example, normalizing can involve multiplying a factor (e.g., correlation or conversion factor) by the amount of the analyte in the capillary blood sample. As used herein, the term “partition” refers to a volume of fluid (e.g. liquid or gas) that is a separated portion of a bulk volume. A bulk volume may be partitioned into any suitable number (e.g. 10<2 >...10<3 >...10<4 >...10<5 >...10<6 >...10<7>, etc.) of smaller volumes (i.e. partitions). Partitions may be separated by a physical barrier or by physical forces (e.g. surface tension, hydrophobic repulsion, etc.). Partitions generated from the larger volume may be substantially uniform in size (monodisperese) or may have non-uniform sizes (polydisperse). 36Docket No. ABBTT-43596.601 Partitions may be produced by any suitable manner (e.g. emulsion, microfluidics, microspray, etc.). Exemplary partitions are droplets. Partitions are referred to as “subvolumes” and “microvolumes.” “Point-of-care device” refers to a device used to provide medical diagnostic testing at or near the point-of-care (namely, typically, outside of a laboratory), at the time and place of patient care (such as in a hospital, physician’s office, urgent or other medical care facility, a patient’s home, a nursing home and / or a long-term care and / or hospice facility). Examples of point-of-care devices include those produced by Abbott Laboratories (Abbott Park, IL) (e.g., i-STAT and i-STAT Alinity, Universal Biosensors (Rowville, Australia) (see US 2006 / 0134713), Axis-Shield PoC AS (Oslo, Norway) and Clinical Lab Products (Los Angeles, USA). As used herein, the term “primer” refers to an oligonucleotide, whether occurring naturally as in a purified restriction digest or produced synthetically, that is capable of acting as a point of initiation of synthesis when placed under conditions in which synthesis of a primer extension product that is complementary to a nucleic acid strand is induced (e.g., in the presence of nucleotides and an inducing agent such as a biocatalyst (e.g., a DNA polymerase or the like) and at a suitable temperature and pH). The primer is typically single stranded for maximum efficiency in amplification, but may alternatively be double stranded. If double stranded, the primer is generally first treated to separate its strands before being used to prepare extension products. In some embodiments, the primer is an oligodeoxyribonucleotide. The primer is sufficiently long to prime the synthesis of extension products in the presence of the inducing agent. The exact lengths of the primers will depend on many factors, including temperature, source of primer and the use of the method. ““Quality control reagents” in the context of immunoassays and kits described herein, include, but are not limited to, calibrators, controls, and sensitivity panels. A “calibrator” or “standard” typically is used (e.g., one or more, such as a plurality) in order to establish calibration (standard) curves for interpolation of the amount of an analyte, such as an antibody or an analyte. Alternatively, a single calibrator, which is near a reference level or control level (e.g., “low”, “medium”, or “high” levels), can be used. Multiple calibrators (i.e., more than one calibrator or a varying amount of calibrator(s)) can be used in conjunction to comprise a “sensitivity panel.” As used herein, a “reaction vessel” refers to a holder or receiver, such as a container, receptacle, tube, a well (e.g., an array of wells), microwell, and / or cartridge, in or upon which an assay is performed. In some embodiments, the reaction vessel is a well or array of wells. In some embodiments, the well or array of wells has a hydrophilic surface. In other embodiments, the well or array of wells has a hydrophobic surface. In some embodiments, when the reaction vessel is a well or array of wells, each well is of sufficient size to accommodate at least one solid support (e.g., such as a bead, microparticle, tube etc.) and to detect one or more analytes of interest in the well(s). “Result” as used herein refers to an item of information obtained by performing an assay. In one aspect, a result is an amount of a biomarker in a test sample (e.g, blood sample). In another 37Docket No. ABBTT-43596.601 aspect, a result is identifying the presence of biomarker in a sample. A result can be visually displayed (e.g., as a readout). “Plasma separation device” as used herein, refers to an apparatus or device that can be used to separate components of whole blood (e.g., blood cells and platelets) from serum, plasma or serum and plasma using a separation system, such as, for example, at least one membrane, filter, synthetic paper (e.g., micropillar scaffolds) or any combination thereof. Separation of whole blood components can produce a fraction of plasma (“plasma fraction”) and a blood cell and platelet fraction. Any plasma separation device known to the skilled artisan can be used to separate components of whole blood. Examples of suitable plasma separation devices of use herein include, without limitation, an automated blood cell separator device, a capillary flow-driven microfluidic device (e.g., for point-of-care plasma separation), a centrifugal microfluidic disk (lab-on-a-disk) device using a separator gel, a centrifuge, a deterministic lateral displacement (DLD) microfluidic chip, a plasma separation card. “Specific binding” or “specifically binding” as used herein may refer to the interaction of an antibody, a protein, or a peptide with a second chemical species, wherein the interaction is dependent upon the presence of a particular structure (e.g., an antigenic determinant or epitope) on the chemical species; for example, an antibody recognizes and binds to a specific protein structure rather than to proteins generally. If an antibody is specific for epitope “A”, the presence of a molecule containing epitope A (or free, unlabeled A), in a reaction containing labeled “A” and the antibody, will reduce the amount of labeled A bound to the antibody. “Specific binding member” is a member of a specific binding pair. A specific binding pair comprises two different molecules, which specifically bind to each other through chemical or physical means. Therefore, in addition to antigen and antibody specific binding pairs of common immunoassays, other specific binding pairs can include biotin and avidin (or streptavidin), carbohydrates and lectins, complementary nucleotide sequences, effector and receptor molecules, cofactors and enzymes, enzymes and enzyme inhibitors, and the like. Furthermore, specific binding pairs can include members that are analogs of the original specific binding members, for example, an analyte-analog. Immunoreactive specific binding members include antigens, antigen fragments, and antibodies, including monoclonal and polyclonal antibodies as well as complexes and fragments thereof, whether isolated or recombinantly produced, and aptamers. “Statistically significant” as used herein refers to the likelihood that a relationship between two or more variables is caused by something other than random chance. Statistical hypothesis testing is used to determine whether the result of a data set is statistically significant. In statistical hypothesis testing, a statistically significant result is attained whenever the observed p-value of a test statistic is less than the significance level defined of the study. The p-value is the probability of obtaining results at least as extreme as those observed, given that the null hypothesis is true. Examples of statistical 38Docket No. ABBTT-43596.601 hypothesis analysis include Wilcoxon signed-rank test, t-test, Chi-Square or Fisher’s exact test. “Significant” as used herein refers to a change that has not been determined to be statistically significant (e.g., it may not have been subject to statistical hypothesis testing). “Subject” and “patient” as used herein interchangeably refers to any vertebrate, including, but not limited to, a mammal (e.g., cow, pig, camel, llama, horse, goat, rabbit, sheep, hamsters, guinea pig, cat, dog, rat, and mouse, a non-human primate (for example, a monkey, such as a cynomolgus or rhesus monkey, chimpanzee, etc.) and a human). In some embodiments, the subject may be a human or a non-human. In some embodiments, the subject is a human. The subject or patient may be undergoing other forms of treatment. As used herein, a “transfer tube” refers to a container or receptacle used to transfer a fluid (e.g., a blood sample) from one location to a second location (e.g., to a reaction vessel or from a plasma separation device). “Venous blood” as used herein refers to a blood sample that is obtained from the veins from a subject using a syringe, needle, or combination thereof, or any appropriate device. In some embodiments, a venous blood sample is obtained by a trained health clinician such as a physician, phlebotomist, nurse, laboratory technician, or combination thereof. In some embodiments, a venous blood sample is whole blood, serum or plasma. Unless otherwise defined herein, scientific and technical terms used in connection with the present disclosure shall have the meanings that are commonly understood by those of ordinary skill in the art. For example, any nomenclatures used in connection with, and techniques of, cell and tissue culture, molecular biology, immunology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those that are well known and commonly used in the art. The meaning and scope of the terms should be clear; in the event, however of any latent ambiguity, definitions provided herein take precedent over any dictionary or extrinsic definition. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. 2. Digital assays using a set of color codes Aspect of the disclosure relates to digital detection assays using a set of color codes. The use of combinations of different color labels or dyes to label nucleic acid probes in nucleic acid assays or detection agents in immunoassays expands the coding space for larger sets of targets. For instance, a red and blue label or dye can be used for two targets and then can be combined for a third target. Because the targets are spread out into separate reaction vessels, it will be possible to see red vessels where only target one is detected, blue vessels where only target two is present, and red and blue in vessels where only target three resides. Additional label or labels or dyes can be included to expand the coding space. 39Docket No. ABBTT-43596.601 The disclosure contemplates the use of any number of labels or dyes to expand the coding space for detecting a larger set of targets. Any combination of two or more different colored labels or dyes can be used to create a code in accordance with an amplification or immunoassay herein. In some embodiments, the disclosure contemplates a set of codes comprising at least two, at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes used individually and / or in any combination of two of the at least two, at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colors. In other embodiments, the disclosure contemplates a set of codes comprising at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes used individually and / or in any combination of two or three of the at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes. In still other embodiments, the disclosure contemplates a set of codes comprising at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes used individually and / or in any combination of two, three, or four of the at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes. In an exemplary embodiment, the amplification assay or immunoassay uses at least two different colored labels or dyes in combination to code for at least a first target nucleic acid or antigen of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least three different colored labels or dyes in combination to code for at least six different target nucleic acid or antigen of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least four different colored labels or dyes in combination to code for at least 10 different target nucleic acid or antigen of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least two different colored labels or dyes individually and / or in combination to code for three different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least three different colored labels or dyes individually and / or in combinations of any two different colors to code for up to six different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least four different colored labels or dyes and / or in combinations of two to code for up to 10 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least five different colored labels or dyes individually and / or in combinations of two to code for up to 15 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least six different colored labels or dyes individually and / or in combinations of two to code for up to 21 different target nucleic acids or antigens of interest. 40Docket No. ABBTT-43596.601 In an exemplary embodiment, the amplification assay or immunoassay uses at least three different colored labels or dyes individually and / or in combinations of two or three different colored labels or dyes to code for up to 7 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least four different colored labels or dyes individually and / or in combinations of two or three different colored labels or dyes to code for up to 13 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least five different colored labels or dyes individually and / or in combinations of two or three different colored labels or dyes to code for up to 24 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay or immunoassay uses at least six different colored labels or dyes individually and / or in combinations of two or three different colored labels or dyes to code for up to 40 different target nucleic acids or antigens of interest. The disclosure contemplates combining the different colored labels or dyes in different ratios to produce the set of color codes. For example, the first and second labels or dyes can be provided in a ratio of first color label or dye to second color label or dye of 1:1, 1:2, 1:3, 1:4, 1:5, or 5:1, 4:1, 3:1, or 2:1. In another example, the first, second, and third color label or dye can be provided in a ratio of first color label or dye:second color label or dye; third color label or dye of 1:1:1, 1:2:1, 1:3:1, 1:4:1, 1:5:1, 1:1:2, 1:1:3, 1:1:4, 1:1:5, 5:1:1, 4:1:1, 3:1:1, 2:1:1, 2:1:2, 3:1:2, 2:1:3, 4:1:2, 2:1:4, :5:1:2, 2:1:5, 5:1:3, 3:1:5, 5:1:4, 4:1:5, 5:1:5, 2:3:2, 2:5:2, 3:1:3, 4:1:4, 4:1:3, 3:1:4, etc. The skilled person will appreciate that additional ratios can be provided similarly for combinations of four labels or dyes, five labels or dyes, six labels or dyes, seven labels or dyes, eight labels or dyes, nine labels or dyes, or ten labels or dyes, as long as the combinations used in any particular reaction provide sufficient contrast to distinguish the resulting color combinations from each other. The disclosure also contemplates any colors for use in the combinations, for example, red, blue, yellow, green, orange, pink, purple, white, black, brown, etc. and combinations thereof can be used. 3. Digital multiplexed amplification assays using a set of color codes In an aspect, the disclosure provides systems, methods and kits for performing a digital multiplexed nucleic acid amplification assay to detect a plurality of target nucleic acids in a sample, for example, using different colored labels or dyes to create a set of color codes specific for different target nucleic acids of interest in the sample. In an embodiment, the disclosure provides a method for performing a digital multiplexed nucleic acid amplification assay to detect at least a first target nucleic acid in a sample, comprising: (a) labeling at least a first nucleic acid probe with at least two different colored labels or dyes to create a set of color codes specific for at least a first target nucleic acid of interest, wherein: (i) the 41Docket No. ABBTT-43596.601 first nucleic acid probe specific for the first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (b) aliquoting the sample into at least one reaction vessel, wherein on average, the at least one reaction vessel contains a single molecule of the target nucleic acid and the first nucleic acid probe; (c) amplifying in the at least one reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in the at least one reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the combination of colored labels or dyes comprising at least the first and second color labels or dyes when the first target nucleic acid is detected in the reaction vessel. In some embodiments, step (a) includes labeling at least three nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least a three target nucleic acid of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a third color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising at least the second color label or dye and at least the third color label or dye. In such embodiments, step (d) includes detecting in the at least one reaction vessel, on average, a single molecule of target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first color label or dye and at least a third color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the second and third color label or dye when the third target nucleic acid is detected in the reaction vessel. In some embodiments, step (a) includes labeling at least 10 nucleic acid probes with at least four different colored labels or dyes to create a set of color codes specific for at least 10 target nucleic acid of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a third color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels dyes comprising at least a first color label or dye and at least a fourth color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising at least the second color label or dye and at least the third color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled 42Docket No. ABBTT-43596.601 with a combination of labels or dyes comprising at least a second color label or dye and at least the fourth color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising at least a third color label or dye and at least the fourth color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising at least the first, second, and third color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising at least the first, second, and fourth color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising at least the first, third, and fourth color label or dye; and (ix) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising at least the second, third, and fourth color label dye. In such embodiments, step (d) includes detecting in the at least one reaction vessel, on average, a single molecule of target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first color label or dye and at least a third color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first and fourth color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the second and third color labels or dyes when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the second and fourth color labels or dyes when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the third and fourth color labels or dyes when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of dyes comprising at least the first, second, and third color labels or dyes when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first, third, and fourth color labels or dyes when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first, second, and fourth color labels or dyes when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the second, third, and fourth color labels or dyes when the tenth target nucleic acid is detected in the reaction vessel. In another embodiment, the disclosure provides a method for performing a digital multiplexed nucleic acid 43Docket No. ABBTT-43596.601 amplification assay to detect a plurality of target nucleic acids in a sample, comprising: (a) labeling at least three different nucleic acid probes with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) aliquoting the sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel contains a single molecule of target nucleic acid and the at least three different nucleic acid probes (many reaction vessels will be empty); (c) amplifying in each reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (iii) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel. In some embodiments, step (a) includes labeling at least six different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least six different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; and (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye. In such embodiments, (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal 44Docket No. ABBTT-43596.601 corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the fifth target nucleic acid is detected in the reaction vessel; and (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the target nucleic acid is detected in the reaction vessel. In other embodiments, step (a) includes labeling at least ten different nucleic acid probes with at least four different colored labels or dyes to create a set of color codes specific for at least ten different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (viii) an eight nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; and (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye. In such embodiments, (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of dyes comprising the first color label or dye and the fourth color label or dye when the 45Docket No. ABBTT-43596.601 seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; and (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel. In still other embodiments, step (a) includes labeling at least 15 different nucleic acid probes with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of dyes comprising the first color label or dye and the second color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or labels or dye; (ix) a ninth nucleic acid probe specific for an ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; and (xv) a fifteenth nucleic acid probe specific for a fifteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye. 46Docket No. ABBTT-43596.601 In such embodiments, (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of dyes comprising the first color label or dye and the third color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fifth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the tenth target nucleic acid is detected in the reaction vessel; (xi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the eleventh target nucleic acid is detected in the reaction vessel; (xii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fifth color label or dye when the twelfth target nucleic acid is detected in the reaction vessel; (xiii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the thirteenth target nucleic acid is detected in the reaction vessel; (xiv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fifth color label or dye when the fourteenth target nucleic acid is detected in the reaction vessel; and (xv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye when the fifteenth target nucleic acid is detected in the reaction vessel. In further embodiments, step (a) includes labeling at least 21 different nucleic acid probes with at least six different colored labels or dyes to create a set of color codes specific for at least 21 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe 47Docket No. ABBTT-43596.601 specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a sixth color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the sixth color label or dye; (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xv) a fifteenth nucleic acid probe specific for a fifteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the sixth color label or dye; (xvi) a sixteenth nucleic acid probe specific for a sixteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xvii) a seventeenth nucleic acid probe specific for a seventeenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; (xviii) an eighteenth nucleic acid probe specific for an eighteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the sixth color label or dye; (xix) a nineteenth nucleic acid probe specific for a nineteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye; (xx) a twentieth nucleic acid probe specific for a twentieth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye; and (xxi) a twenty- first nucleic acid probe specific for a twenty-first target nucleic acid is labeled with a combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye. In such embodiments, (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target 48Docket No. ABBTT-43596.601 nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the sixth color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fifth color label or dye when the tenth target nucleic acid is detected in the reaction vessel; (xi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the sixth color label or dye when the eleventh target nucleic acid is detected in the reaction vessel; (xii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the twelfth target nucleic acid is detected in the reaction vessel; (xiii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the thirteenth target nucleic acid is detected in the reaction vessel; (xiv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fifth color label or dye when the fourteenth target nucleic acid is detected in the reaction vessel; and (xv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the sixth color label or dye when the fifteenth target nucleic acid is detected in the reaction vessel; (xvi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the sixteenth target nucleic acid is detected in the reaction vessel; (xvii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fifth color label or dye when the seventeenth target nucleic acid is detected in the reaction vessel; (xviii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the sixth color label or dye when the eighteenth target nucleic acid is detected in the reaction vessel (xix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the 49Docket No. ABBTT-43596.601 fifth color label or dye when the nineteenth target nucleic acid is detected in the reaction vessel; (xx) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye when the twentieth target nucleic acid is detected in the reaction vessel; (xxi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye when the thirtieth target nucleic acid is detected in the reaction vessel. In certain embodiments, step (a) comprises labeling at least seven different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least seven different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; and (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye, the second color label or dye, and the third color label or dye. 4. Amplification In some embodiments, the present disclosure provides compositions and methods for the amplification of target nucleic acids (e.g. DNA, RNA, etc.). In some embodiments, amplification is performed on a sample that has been divided into partitions (e.g. droplets). In some embodiments, an amplification reaction is carried out within each partition. In some embodiments, amplification is performed on a sample that has been aliquoted, for example, into a reaction vessel (e.g., an aliquot). In some embodiments, the sample is aliquoted into a transfer tube. In some embodiments, a partition, container, or reaction vessel (e.g., aliquot) contains all the reagents necessary for nucleic acid amplification (e.g. primers, polymerase, deoxynucleotides, template (e.g. target, internal standard, etc.)). In some embodiments, amplification is performed using digital PCR (dPCR) methods. In some other embodiments, the amplification is performed using dPCR methods where the number of subvolumes containing 0 target is nonexistent or is too low to apply Poisson statistics. In some embodiments, the present disclosure provides compositions (e.g. primers, buffers, salts, nucleic acid targets, etc.) and methods for the amplification of nucleic acid (e.g. dPCR, digital 50Docket No. ABBTT-43596.601 droplet amplification, PCR amplification, partitioned amplification, cdPCR, dPCR LATE, combinations thereof, etc.). In some embodiments, an amplification reaction is any reaction in which nucleic acid replication occurs repeatedly over time to form multiple copies of at least one segment of a template or target nucleic acid molecule (e.g. DNA, RNA). In some embodiments, amplification reaction is achieved by repeated thermal cycling. In some embodiments, amplification reaction is achieved isothermally. In some embodiments, amplification generates an exponential or linear increase in the number of copies of the template nucleic acid. Amplifications may produce in excess of a 1,000-fold increase in template copy-number and / or target-detection signal. Exemplary amplification reactions include, but are not limited to, the polymerase chain reaction (PCR) or ligase chain reaction (LCR), each of which is driven by thermal cycling. Amplifications used in method or assays of the present disclosure may be performed in bulk and / or partitioned volumes (e.g. droplets, reactive vessels, etc.). Alternative amplification reactions, which may be performed isothermally, also find use herein, such as signal amplification, branched-probe DNA assays, cascade-RCA, helicase-dependent amplification, loop-mediated isothermal amplification (LAMP), nucleic acid-based amplification (NASBA), nicking enzyme amplification reaction (NEAR), PAN-AC, Q-beta replicase amplification, rolling circle replication (RCA), self- sustaining sequence replication, strand-displacement amplification, and the like. Amplification may be performed with any suitable reagents (e.g., template nucleic acid (e.g. DNA or RNA), primers, probes, buffers, replication catalyzing enzyme (e.g., DNA polymerase, RNA polymerase), nucleotides, salts (e.g., MgCl2), etc. In some embodiments, an amplification mixture includes any combination of at least one primer or primer pair, at least one probe, at least one replication enzyme (e.g., at least one polymerase, such as at least one DNA and / or RNA polymerase), and deoxynucleotide (and / or nucleotide) triphosphates (dNTPs and / or NTPs), etc. In some embodiments, the probes are labeled with a set of codes comprising at least two, at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes used individually and in any combination of two of the at least two, at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colors. In other embodiments, the probes are labeled with a set of codes comprising at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes used individually and in any combination of two or three of the at least three, at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes. In still other embodiments, the probes are labeled set of codes comprising at least four, at least five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes used individually and in any combination of two, three, or four of the at least three, at least four, at least 51Docket No. ABBTT-43596.601 five, or at least six, or at least seven, or at least eight, or at least nine, or at least 10 different colored labels or dyes. In an exemplary embodiment, the amplification assay comprises probes labeled with at least two different colored labels or dyes individually and in combination to code for three different target nucleic acids. In some aspects, a probe may be labeled with at least two different colored labels or dyes. That is, all the probes in a probe population used in an amplification assay are labeled with at least two different colored labels or dyes. In other aspects, certain amounts or percentages of probes in a probe population are labeled with a first colored label or dye and the remaining amounts or percentages of probes in the probe population are labeled with a second color label or dye which is different than the first colored label or dye. Optionally, three, four, five, or six different colored labels or dyes could be used. For example, in some aspects, half of the target specific probe population could be labeled with one color label or dye (a first colored label or dye) and the other half of the target specific probe population could be labeled with a second color label or dye which is different than the first colored label or dye. By way of another example in some aspects, one-third of the target specific probe population could be labeled with one color label or dye (a first colored label or dye), another one-third of the target specific probe population could be labeled with a second color label or dye which is different than the first colored label or dye, and the remaining one-third of the target specific probe population could be labeled with a third color label or dye which is different than the first and second color labels or dyes. By way of another example in some aspects, one-quarter of the target specific probe population could be labeled with one color label or dye (a first colored label or dye), another one-quarter of the target specific probe population could be labeled with a second color label or dye which is different than the first colored label or dye, one-quarter of the target specific probe population could be labeled with a third color label or dye which is different than the first and second color labels or dyes, and the remaining one-quarter of the target specific probe population could be labeled with a fourth color label or dye which is different than the first-third color labels or dyes. In an exemplary embodiment, the amplification assay comprises probes labeled with at least three different colored labels or dyes individually and in combinations of any two different colors to code for up to six different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at least four different colored labels or dyes individually and in combinations of two to code for up to 10 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at least five different colored labels or dyes individually and in combinations of two to code for up to 15 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at least six different colored labels or dyes individually and in combinations of two to code for up to 21 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at 52Docket No. ABBTT-43596.601 least three different colored labels or dyes individually and in combinations of two or three different colored labels or dyes to code for up to 7 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at least four different colored labels or dyes individually and in combinations of two or three different colored labels or dyes to code for up to 13 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at least five different colored labels or dyes individually and in combinations of two or three different colored labels or dyes to code for up to 24 different target nucleic acids or antigens of interest. In an exemplary embodiment, the amplification assay comprises probes labeled with at least six different colored labels or dyes individually and in combinations of two or three different colored labels or dyes to code for up to 40 different target nucleic acids or antigens of interest. In some embodiments, the present disclosure utilizes nucleic acid amplification that relies on alternating cycles of heating and cooling (i.e., thermal cycling) to achieve successive rounds of replication (e.g., PCR). In some embodiments, PCR is used to amplify target nucleic acids (e.g. partitioned targets). PCR may be performed by thermal cycling between two or more temperature set points, such as a higher melting (denaturation) temperature and a lower annealing / extension temperature, or among three or more temperature set points, such as a higher melting temperature, a lower annealing temperature, and an intermediate extension temperature, among others. PCR may be performed with a thermostable polymerase, such as Taq DNA polymerase (e.g., wild-type enzyme, a Stoffel fragment, FastStart polymerase, etc.), Pyrococcus furiosus (Pfu) DNA polymerase, S-Tbr polymerase, Thermus thermophilus (Tth) polymerase, Vent polymerase, or a combination thereof, among others. Typical PCR methods produce an exponential increase in the amount of a product amplicon over successive cycles, although linear PCR methods also find use in the present disclosure. Any suitable PCR methodology, combination of PCR methodologies, or combination of amplification techniques may be utilized in the partitioned methods (e.g. droplet-based detection, separation, and / or sequencing of target nucleic acids, reaction vessels, etc.) disclosed herein, such as cdPCR, dPCR LATE, allele-specific PCR, assembly PCR, asymmetric PCR, digital PCR, endpoint PCR, hot-start PCR, in situ PCR, intersequence-specific PCR, inverse PCR, linear after exponential PCR, ligation-mediated PCR, methylation-specific PCR, mini-primer PCR, multiplex ligation- dependent probe amplification, multiplex PCR, nested PCR, overlap-extension PCR, polymerase cycling assembly, qualitative PCR, quantitative PCR, real-time PCR, RT-PCR, single-cell PCR, solid- phase PCR, thermal asymmetric interlaced PCR, touchdown PCR, or universal fast walking PCR, etc. In some embodiments, the present disclosure provides dPCR methods (See, e.g., Ramakrishnan, American Biotechnology Laboratory 27(8), 11-13 (2009); herein incorporated by reference in its entirety). In some embodiments, PCR is performed on portions of a sample (e.g. 53Docket No. ABBTT-43596.601 partitions) to determine the presence or absence, concentration, and / or copy number of a nucleic acid target in the sample, based on how many of the sample portions support amplification of the target. In some embodiments, PCR is performed on portions of a sample (e.g. partitions or reaction vessels) to determine the presence or absence, concentration, and / or copy number of a nucleic acid target in the sample, based on quantitation of sub-volume nucleic acid concentration determined by methods described herein (e.g., cdPCR, dPCR LATE). In some embodiments, PCR is performed on portions of a sample (e.g. partitions) to detect more than one target nucleic acid and / or to determine the concentration, and / or relative concentrations of multiple target nucleic acids within a sample. In some embodiments, digital PCR is performed as endpoint PCR (e.g., for each of the partitions, reaction vessels, etc.). In some embodiments, digital PCR is performed as rtPCR (e.g., for each of the partitions, reaction vessels, etc.). In some embodiments, digital PCR is performed as dPCR LATE (e.g., for each of the partitions, reaction vessels, etc.). In some embodiments, digital PCR is performed as cdPCR (e.g., for each of the partitions). Conventional PCR theoretically results in an exponential amplification of a nucleic acid sequence (e.g. template or target nucleic acid) from a sample over a series of cycles in the early portion of the amplification process. By measuring the number of amplification cycles required to achieve a threshold level of amplification (as in real-time PCR), the starting concentration of nucleic acid can be calculated. However, there are many factors that affect the exponential amplification of the PCR process, such as varying amplification efficiencies, competition of low copy number targets with higher concentration targets for reagents, and interference with background contaminant nucleic acid. Digital PCR is generally less sensitive to these factors, since it does not rely on the assumption that the PCR process is exponential. In digital PCR, individual nucleic acid molecules from the initial sample are distributed amongst the partitions, and then amplified to detectable levels. The distribution of these target nucleic acid molecules across the partitions is governed by Poisson statistics. Quantitation in dPCR is possible whenever there is a sufficient number of 0 target containing partitions to apply Poisson statistics. As the number of 0 target containing partitions decreases approaching zero, the uncertainty in the quantitation value increases, ultimately becoming undefined (infinity) when there are no 0 target containing partitions. In embodiments in which multiple target nucleic acids are analyzed, digital PCR provides statistically relevant measure of the concentrations or ratios to multiple target nucleic acids. In some embodiments, the present disclosure provides dPCR methods, or elements thereof. In some embodiments, the present disclosure provides LATE PCR (U.S. Patent No. 7,632,642; herein incorporated by reference in its entirety), or elements thereof. In some embodiments LATE PCR methods are performed in a digital format (e.g. dPCR LATE). In some embodiments, LATE PCR methods are performed on a partitioned sample. In some embodiments, LATE PCR techniques are utilized for amplification of a partitioned sample (e.g. digital or non-digital sample). In 54Docket No. ABBTT-43596.601 some embodiments, LATE-PCR or dPCR LATE is a non-symmetric DNA amplification employing the polymerase chain reaction (PCR) process utilizing one oligonucleotide primer (the “Excess Primer”) in at least five-fold excess with respect to the other primer (the “Limiting Primer”), which itself is utilized at low concentration, up to 200 nM, so as to be exhausted in roughly sufficient PCR cycles to produce fluorescently detectable double-stranded amplicon, wherein the concentration- adjusted melting temperature of the Limiting Primer at the start of amplification, Tm[0]<L>, is not more than 5° C. below the concentration-adjusted melting temperature of the Excess Primer at the start of amplification, Tm[0]<X>, preferably at least as high and more preferably 3-10° C. higher; and wherein thermal cycling is continued for multiple cycles after exhaustion of the Limiting Primer to produce single-stranded product, namely, the extension product of the Excess Primer, sometimes referred to as the “Excess Primer Strand”. In some embodiments, the present disclosure provides competitive PCR (e.g. cdPCR), or elements thereof. Conventional competitive PCR is a method for target quantitation using end point PCR, which employs the co-amplification of an unknown and IS target sequences that share primer sequences. Since both amplifications consume the same primers, they compete for the available resources. If each reaction has the same efficiency, the relationship of the initial (pre-amplification) concentration of unknown (T0) and IS (IS0) to the final post amplification concentration after j cycles (Tj and ISj) is described by the expression: Log(Tj / ISj)=log(T0)−log(IS0) In conventional competitive PCR applications, utilization of this method requires a priori knowledge of IS0 or a calibration curve to establish the relationship between measured response and unknown concentration. The calibration curve is expressed as: log (target response / internal standard response) vs. log ([target]). This response produces a relatively straight-line curve that allows for subsequent reactions to be quantitated. The curve deviates from linearity based on how similar the amplification efficiencies are for the 2 competitive targets. In general, this method can produce up to approximately 5 logs of quantitation depending on the precision of the response measurements. The problems for quantitation associated with the conventional competitive PCR method include limited dynamic range and imprecision at the extremes of the quantitative range. In addition, this method requires generation of the calibration curve described above due to variation in IS concentration from lot to lot, reaction to reaction, and other factors. In some embodiments, these challenges are overcome by performing competitive PCR in a digital format. In some embodiments, the present disclosure utilizes real-time PCR, or elements thereof. In some embodiments, real-time PCR achieves quantitation by reading the fluorescence response of the target PCR reaction at frequent cycle intervals (e.g. every cycle), and identifying the cycle number at which the signal crosses a threshold response value. Quantitation is based on the efficiency of 55Docket No. ABBTT-43596.601 amplification and the number of cycles or amplification events required to produce a detectable concentration of product. The problems for quantitation associated with real-time PCR largely relate to determination of low copy targets in the presence of other amplifications, such as for other high titer targets or nonspecific amplifications, e.g., primer dimers. Other challenges include amplification in the presence of non-amplifying nucleic acid (e.g. genomic DNA) that can interfere with the amplification process and suppress low copy amplification responses. Real-time PCR also requires some type of calibration, typically either a calibration curve run within the batch or stored, or a reference quantitation standard (QS) of known concentration run in every reaction that defines the relationship between cycle number and concentration. In some embodiments, the present disclosure provides amplification techniques (e.g. dPCR LATE, cdPCR, combinations thereof, etc.) capable of extending the dynamic range for dPCR and achieving precise quantitation at and beyond the upper end of the quantitative range. In some embodiments, amplification and analysis methods described herein (e.g. dPCR LATE, cdPCR, combinations thereof, etc.) provide an extension of the dPCR dynamic range to as much as much as 8 orders of magnitude (e.g.5 orders of magnitude ...6 orders of magnitude ...7 orders of magnitude . ..8 orders of magnitude). In some embodiments, amplification and analysis methods described herein (e.g. dPCR LATE, cdPCR, combinations thereof, etc.) provide an extension of the dPCR dynamic range by up to three logs over what is obtainable by Poisson analysis (e.g., extension of 1 order of magnitude, extension of 2 orders of magnitude, extension of 3 orders of magnitude). 5. Amplicon detection / quantification In some embodiments, a sample is partitioned using any suitable method known in the art, and a nucleic acid amplification procedure (e.g. cdPCR, dPCR LATE, etc.) is performed to amplify target nucleic acids present in one or more of the partitions. The one or more partitions may include probes comprising a set of color codes that are specific for a single target nucleic acid of interest. In some embodiments, a sample is aliquoted into a series of tubes or reaction vessels using any suitable method known in the art, and a nucleic acid amplification procedure (e.g., cdPCR, dPCR, LATE, isothermal amplification, etc.) is performed to amplify target nucleic acids present in one or more of the tubes or reaction vessels. The one or more tubes or reaction vessels may include probes comprising a set of color codes that are specific for a single target nucleic acid of interest. In some embodiments, a quantification method is provided to quantify the amplicons produced by the amplification reactions in each partition. In some embodiments, quantification is performed following amplification (e.g., end-point detection). In some embodiments quantification is performed in real time (e.g. amplicon quantification following each round of amplification). In some embodiments, the present disclosure provides systems, devices, methods, and compositions to detect the presence of and / or quantify nucleic acids (e.g. amplicons (e.g. IS amplicons, target amplicons), 56Docket No. ABBTT-43596.601 labeled nucleic acids) in a sample or partition. In some embodiments, the present disclosure provides detection of the presence of amplicons in partitions. In some embodiments, the present disclosure provides quantification of amplicons (e.g. IS amplicons, target amplicons) in a partition.. In some embodiments, amplicon quantification involves measurement or detection of a characteristic of partitions and / or amplicons, such as a physical, chemical, luminescence, or electrical aspect, which correlates with amplification (e.g. fluorescence, luminescence, radioactivity, or other detectable signal) and allows amplicon quantification. In some embodiments, amplicon quantification is performed by a fluorescence detection technique. In some embodiments, fluorescence detection methods are provided for detection / quantification of amplified nucleic acids. In addition to the reagents already discussed, and those known to those of skill in the art of nucleic acid amplification and quantification, various detection reagents, such as fluorescent and non-fluorescent labels or dyes and probes are provided. In some embodiments, detection methods are provided for detection / quantification of amplified nucleic acids comprising a set of color codes described herein for detecting on average a single molecule of a target nucleic acid in each reaction vessel or partition. For example, the protocols may employ reagents suitable for use in a TaqMan™ reaction, such as a TaqMan™ probe; reagents suitable for use in a molecular beacon reaction, such as molecular beacon probes; reagents suitable for use in a scorpion reaction, such as a scorpion probe; reagents suitable for use in a fluorescent DNA-binding dye-type reaction, such as a fluorescent probe; and / or reagents for use in a LightUp protocol, such as a LightUp probe. In some embodiments, the present disclosure provides methods and compositions for quantifying a detectable signal (e.g. fluorescence) from partitions containing amplified nucleic acid (e.g. target amplicons, IS amplicons, etc.). Thus, for example, methods may employ labeling (e.g. during amplification, post-amplification) amplified nucleic acids with a detectable label, exposing partitions to a light source at a wavelength selected to cause the amplicon bound probe dye to fluoresce, and detecting and / or measuring the resulting fluorescence. Fluorescence emitted from the partitions can be tracked during amplification reaction to permit monitoring of the reaction, or fluorescence can be measured post-amplification. In some embodiments, the present disclosure provides methods of detecting and / or quantifying the presence of a target nucleic acid in partitions by providing a probe with specificity for a target nucleic acid (e.g., a TaqMan™-type probe) in partitioned amplification reactions, and detecting / measuring the resulting fluorescence. In some embodiments, partitions containing amplified nucleic acid (e.g. target amplicons, IS amplicons, etc.) will exhibit quantifiable post-amplification fluorescence. In some embodiments, detection of a fluorescent signal is indicative of the presence of the template nucleic acid (e.g. IS, target) in the partition. The present disclosure provides corresponding methods for using other suitable target- specific probes (e.g. different colored labels or dyes comprising a set of color codes specific for target 57Docket No. ABBTT-43596.601 nucleic acids of interest, intercalation labels or dyes, scorpion probes, molecular beacons, etc.), as would be understood by one of skill in the art. In some embodiments, the present disclosure provides quantification of amplified nucleic acids using one or more of fluorescent labeling, fluorescent intercalation labels or dyes, FRET-based detection methods (U.S. Patent No.5,945,283; PCT Publication WO 97 / 22719; both of which are incorporated by reference in their entireties), quantitative PCR, real-time fluorogenic methods (U.S. Patent No.5,210,015 to Gelfand, U.S. Patent No.5,538,848 to Livak, et al., and U.S. Patent No.5,863,736 to Haaland, as well as Heid, C. A., et al., Genome Research, 6:986-994 (1996); Gibson, U. E. M, et al., Genome Research 6:995-1001 (1996); Holland, P. M., et al., Proc. Natl. Acad. Sci. USA 88:7276-7280, (1991); and Livak, K. J., et al., PCR Methods and Applications 357-362 (1995), each of which is incorporated by reference in its entirety), molecular beacons (Piatek, A. S., et al., Nat. Biotechnol.16:359-63 (1998); Tyagi, S. and Kramer, F. R., Nature Biotechnology 14:303-308 (1996); and Tyagi, S. et al., Nat. Biotechnol.16:49-53 (1998); herein incorporated by reference in their entireties), Invader™ assays (Third Wave Technologies, (Madison, Wis.)) (Neri, B. P., et al., Advances in Nucleic Acid and Protein Analysis 3826:117-125, 2000; herein incorporated by reference in its entirety), nucleic acid sequence-based amplification (NASBA; (See, e.g., Compton, J. Nucleic Acid Sequence-based Amplification, Nature 350: 91-91, 1991.; herein incorporated by reference in its entirety), Scorpion probes (Thelwell, et al. Nucleic Acids Research, 28:3752-3761, 2000; herein incorporated by reference in its entirety), capacitive DNA detection (See, e.g., Sohn, et al. (2000) Proc. Natl. Acad. Sci. U.S.A.97:10687-10690; herein incorporated by reference in its entirety), etc. In some embodiments, labeling methods are used to differentially label different amplicons. In some embodiments, IS amplicons and target amplicons are differentially labeled to allow for separate detection and quantification. In some embodiments, IS amplicons and target amplicons are differentially labeled to allow for sorting of IS-containing partitions from partitions without IS amplicons. In some embodiments, different target sequences are differentially labeled to allow for separate detection and quantification. In some embodiments, different target sequences are differentially labeled to allow for sorting of one target from another. In some embodiments, differential labeling is achieved via sequence specific labeling probes with detectably different fluorescent labels. In some embodiments, the amplicons are differentially labeled using a set of color codes comprising different colored dlabels or dyes as described herein. 6. Digital multi-parallel immunoassays using a set of color codes In another aspect, the present disclosure further provides systems, methods and kits for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, for example, using the set of color codes specific for different target antigens of interest in the sample. 58Docket No. ABBTT-43596.601 In an embodiment, the disclosure provides a method for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, comprising: (a) labeling at least a first detection agent with at least two different color labels or dyes to create a set of color codes specific for at least a first target antigens of interest, wherein: (i) the first detection agent specific for the first target antigen is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye, and (b) performing a digital immunoassay in parallel for at least the first target antigen to detect, on average, a single one of at least the first target antigen of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen. In some embodiments, step (a) includes labeling at least four detection agent with at least three different color labels or dyes to create a set of color codes specific for at least four different target antigens of interest, wherein: (i) a first detection agent specific for the first target antigen is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a combination of labels or dyes comprising at least the first color label or dye and at least a third color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising at least the second and third color label or dyes; (iv) and fourth detection agent specific for a fourth target antigen is labeled with a combination of labels or dyes comprising at least the first, second, and third color label or dyes. In such embodiments, step (b) includes performing a digital immunoassay in parallel for at least the three target antigens to detect, on average, a single one of at least the three target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and third color labels or dyes when at least the first detection agent detects at least the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the second and third color labels or dyes when at least the third detection agent detects at least the third target antigen; and (iv) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first, second, and third color labels or dyes when at least the fourth detection agent detects at least the fourth target antigen. In some embodiments, step (a) includes labeling at least 10 detection agents with at least four different color labels or dyes to create a set of color codes specific for at least 10 different target antigens of interest, wherein: (i) a first detection agent specific for the first target antigen is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second 59Docket No. ABBTT-43596.601 color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a combination of labels or dyes comprising at least the first color label or dye and at least a third color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising at least the first color label or dye and at least a fourth color label or dyes; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of dyes comprising at least the second color label or dye and at least a third color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising at least the second and fourth color label or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising at least the third and fourth color label or dyes; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising at least the first, second and third color label or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising at least the first, second and fourth color label or dyes;(ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising at least the first, third and fourth color label or dyes; and (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising at least the second, third and fourth color label or dyes. In such embodiments, step (b) includes performing a digital immunoassay in parallel for at least the 10 target antigens to detect, on average, a single one of at least the 10 target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and third color labels or dyes when at least the first detection agent detects at least the second target antigen; and (iii) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and fourth color labels or dyes when at least the third detection agent detects at least the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the second and third color labels or dyes when at least the fourth detection agent detects at least the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the second and fourth color labels or dyes when at least the fifth detection agent detects at least the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the third and fourth color labels or dyes when at least the sixth detection agent detects at least the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first, second and third color labels or dyes when at least the seventh detection agent detects at least the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the 60Docket No. ABBTT-43596.601 combination of labels or dyes comprising at least the first, second, and fourth color labels or dyes when at least the seventh detection agent detects at least the seventh target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first, third and fourth color labels or dyes when at least the ninth detection agent detects at least the ninth target antigen; and (x) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the second, third and fourth color labels or dyes when at least the tenth detection agent detects at least the tenth target antigen. In yet another embodiment, the disclosure provides a method for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, comprising: (a) labeling at least three different detection agents with at least two different color labels or dyes to create a set of color codes specific for at least three different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye, (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; and (iii) a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) performing a digital immunoassay in parallel for each of the at least three different target antigens to detect, on average, a single one of the at least three different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; and (iii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the third detection agent detects the third target antigen. In some embodiments, step (a) includes labeling at least six different detection agents with at least three different colored labels or dyes to create a set of color codes specific for at least six different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dyes; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first color and third color label or dyes; and (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye. In such embodiments, step (b) can include performing a digital immunoassay in parallel for each of the at least six different target antigens to detect, on average, a single one of the at least six 61Docket No. ABBTT-43596.601 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the fifth detection agent detects the fifth target antigen; and (vi) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the sixth detection agent detects the sixth target antigen. In other embodiments, step (a) can include labeling at least 10 different detection agents with at least four different colored labels or dyes to create a set of color codes specific for at least 10 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the first and third color label or dyes; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first and fourth color label or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the second and third color label or dyes; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of dyes comprising the second and fourth color label or dyes; and (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the third and fourth color label or dyes. In such embodiments, step (b) can include: performing a digital immunoassay in parallel for each of the at least 10 different target antigens to detect, on average, a single one of the at least 10 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal 62Docket No. ABBTT-43596.601 corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the sixth detection agent detects the sixth target antigen (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) wherein the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the eighth detection agent detects the eighth target antigen; (xix) wherein the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the ninth detection agent detects the ninth target antigen; and (x) wherein the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the tenth detection agent detects the tenth target antigen. In still other embodiments, step (a) includes labeling at least 15 different detection agents with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a fifth color label or dye; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of dyes comprising the first and second color label or dyes; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first and third color label or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the first and fourth color label or dyes; (ix) a ninth detection agent specific for an ninth target antigen is labeled with a combination of labels or dyes comprising the first and fifth color label or dyes; (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the second color and third color label or dyes; (xi) an eleventh detection agent specific for an eleventh target antigen is labeled with a combination of labels or dyes comprising the second and fourth color label or dyes; (xii) a twelfth detection agent specific for a twelfth target antigen is labeled with a combination of labels or dyes comprising the second and the fifth color label or dyes; (xiii) a thirteenth detection agent specific for a thirteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xiv) a fourteenth detection agent specific for a fourteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or 63Docket No. ABBTT-43596.601 dye; and (xv) a fifteenth detection agent specific for a fifteenth target antigen is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye. In such embodiments, the method step (b) includes: performing a digital immunoassay in parallel for each of the at least 15 different target antigens to detect, on average, a single one of the at least 15 different target antigens of interest in each immunoassay,: (i) wherein the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) wherein the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) wherein the digital immunoassay emits a light signal corresponding to the fifth color label or dye when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the first and fifth color labels or dyes when the ninth detection agent detects the ninth target antigen; (x) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the tenth detection agent detects the tenth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the eleventh detection agent detects the eleventh target antigen; (xii) the digital immunoassay emits a light signal corresponding to the combination of the second and fifth color labels or dyes when the twelfth detection agent detects the twelfth target antigen; (xiii) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the thirteenth detection agent detects the thirteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the third and fifth color labels or dyes when the fourteenth detection agent detects the fourteenth target antigen; and (xv) the digital immunoassay emits a light signal corresponding to the combination of the fourth and fifth color labels or dyes when the fifteenth detection agent detects the fifteenth target antigen. In certain embodiments, step (a) includes labeling at least 21 different detection agents with at least six different colored labels or dyes to create a set of color codes specific for at least 21 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target 64Docket No. ABBTT-43596.601 antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a fifth color label or dye;(vi) a sixth detection agent specific for a fifth target antigen is labeled with a sixth color label or dye; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of dyes comprising the first color label or dye and the third color label or dye; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; and (xi) an eleventh detection agent specific for an eleventh target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the sixth color label or dye; (xii) a twelfth detection agent specific for a twelfth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xiii) a thirteenth detection agent specific for a thirteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xiv) a fourteenth detection agent specific for a fourteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xv) a fifteenth detection agent specific for a fifteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the sixth color label or dye; (xvi) a sixteenth detection agent specific for a sixteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xvii) a seventeenth detection agent specific for a seventeenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; (xviii) an eighteenth detection agent specific for an eighteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the sixth color label or dye; (xix) a nineteenth detection agent specific for a nineteenth target antigen is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye; (xx) a twentieth detection agent specific for a twentieth target antigen is labeled with a combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye; and (xxi) a twenty-first detection agent specific for a twenty-first target antigen is labeled with a combination of dyes comprising the fifth color label or dye and the sixth color label or dye. In some such embodiments, step (b) includes performing a digital immunoassay in parallel for each of the at least 21 different target antigens to detect, on average, a single one of the at least 21 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first 65Docket No. ABBTT-43596.601 target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the fifth color label or dye when the fifth detection agent detects the fifth target antigen; (vi) wherein the digital immunoassay emits a light signal corresponding to the sixth color label or dye when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the ninth detection agent detects the ninth target antigen; (x) the digital immunoassay emits a light signal corresponding to the combination of the first and fifth color labels or dyes when the tenth detection agent detects the tenth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the first and sixth color labels or dyes when the eleventh detection agent detects the eleventh target antigen; (xii) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the twelfth detection agent detects the twelfth target antigen; (xiii) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the thirteenth detection agent detects the thirteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the second and fifth color labels or dyes when the fourteenth detection agent detects the fourteenth target antigen; (xv) the digital immunoassay emits a light signal corresponding to the combination of the second and sixth color labels or dyes when the fifteenth detection agent detects the fifteenth target antigen; (xvi) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the sixteenth detection agent detects the sixteenth target antigen; (xvii) the digital immunoassay emits a light signal corresponding to the combination of the third and fifth color labels or dyes when the seventeenth detection agent detects the seventeenth target antigen; (xviii) the digital immunoassay emits a light signal corresponding to the combination of the third and sixth color labels or dyes when the eighteenth detection agent detects the eighteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the fourth and fifth color labels or dyes when the nineteenth detection agent detects the nineteenth target antigen; (xx) the digital immunoassay emits a light signal corresponding to the combination of the fourth and sixth color labels or dyes when the twentieth detection agent detects the twentieth target antigen; and (xxi) the digital immunoassay emits a light signal corresponding to the combination of 66Docket No. ABBTT-43596.601 the fifth and sixth color labels or dyes when the twenty-first detection agent detects the twenty-first target antigen. In further embodiments, step (a) includes labeling at least seven different detection agents with at least three different colored labels or dyes to create a set of color codes specific for at least seven different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dyes; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first color and third color label or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the second and third color label or dyes; and (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first, second, and the third color label or dyes. In such embodiments, step (b) includes: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) wherein the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the sixth detection agent detects the sixth target antigen; and (vii) the digital immunoassay emits a light signal corresponding to the combination of the first, second, and third color labels or dyes when the seventh detection agent detects the seventh target antigen. Any digital immunoassay may be utilized. The immunoassay may be an enzyme-linked immunoassay (ELISA), radioimmunoassay (RIA), a competitive inhibition assay, such as forward or reverse competitive inhibition assays, or a fluorescence polarization assay, , for example. The ELISA may be a sandwich ELISA. Specific immunological binding of the antibody to the marker can be detected via direct labels, such as fluorescent or luminescent tags, metals and radionuclides attached to the antibody or via indirect labels, such as alkaline phosphatase or horseradish peroxidase. 67Docket No. ABBTT-43596.601 In an embodiment, the presence or concentration of analyte can be measured or determined using an immunoassay. The disclosure contemplates performing multiple immunoassays in parallel. For example, the methods described herein can perform at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, at least 27, at least 28, at least 29, at least 30, at least 31, at least 32, at least 33, at least 34, at least 35, at least 36, at least 37, at least 38, at least 39, at least 40, at least 41, at least 42, at least 43, at least 44, at least 45, at least 46, at least 47, at least 48, at least 49, or at least 50, at least 60, at least 65, at least 70, at least 75, at least 80, at least 85, at least 90, at least 95, or at least 100 immunoassays in parallel to each other using different colored labels or dyes individually and in combinations of two, three, four, five, six, seven, eight, nine, or 10 different combinations of colors to label detection agents that are specific for different target antigens of interest. The disclosure contemplates performing the immunoassays in parallel such that, on average, only a single target antigen of interest is detected in each immunoassay. In some embodiments, each digital immunoassay is performed in a separate reaction vessel. In some embodiments, the reaction vessel comprises a capture agent bound to a solid support suspended or dispersed in the reaction vessel. In other embodiments, the reaction vessel comprises a capture agent bound to the reaction vessel. In still other embodiments, each immunoassay is performed in a partitioned sample (e.g., droplet, for example, using a digital microfluidic system or method described herein). The disclosure contemplates the use of any capture agent in the digital immunoassays described herein. In some embodiments, the capture agents comprise aptamers. In other embodiments, the capture agents comprise antibodies. In one example, the method may include: (a) detecting the presence of or determining the concentration of an analyte by: (i) contacting the biological sample with at least one first antibody (“at least one first capture antibody”) wherein the capture antibody specifically binds to an epitope on an analyte (e.g., protein) or a fragment thereof to form a capture antibody-analyte complex; (ii) contacting the capture antibody-analyte complex with at least one second antibody comprising at least one detectable label (“at least one first detection antibody”), wherein the detection antibody specifically binds to an epitope on analyte that is not bound by the capture antibody and forms a capture antibody-analyte-detection antibody complex; and (iii) determining the presence or level of analyte in the test sample based on the signal generated by the detectable label in the capture antibody- analyte-detection antibody complex formed in (a)(ii). It should be appreciated that in this example the detectable label comprises a colored dye that is coded specifically for the target antigen of interest (e.g., analyte). 68Docket No. ABBTT-43596.601 In another example, the method may include: (a) detecting the presence of or determining the concentration of analyte by: (i) contacting the biological sample with at least one first antigen (“at least one first capture antigen”) wherein the capture antigen specifically binds to an epitope on the analyte (e.g., an antibody) or a fragment thereof to form a capture antigen-analyte complex; (ii) contacting the capture antigen-analyte complex with at least one second antigen comprising at least one detectable label (“at least one first detection antigen”), wherein the detection antigen specifically binds to an epitope on the analyte that is not bound by the capture antigen and forms a capture antigen-analyte- detection antigen complex; and (iii) determining the presence or level of analyte in the test sample based on the signal generated by the detectable label in the capture antigen-analyte-detection antigen complex formed in (a)(ii). In this example, the detectable label comprises a colored dye that is coded specifically to detect the analyte (e.g., antigen of interest) and the signal generated corresponds to the color of the dye used. The use of immobilized antibodies or fragments thereof or antigens or fragments thereof may be incorporated into the immunoassay. The antibodies or antigens may be immobilized onto a variety of supports, such as magnetic or chromatographic matrix particles, the surface of an assay plate (such as microtiter wells), pieces of a solid substrate material, and the like. An assay strip can be prepared by coating the antibody, plurality of antibodies, antigen or plurality of antigens in an array on a solid support. This strip can then be dipped into the test biological sample and then processed quickly through washes and detection steps to generate a measurable signal, such as a colored spot. Optionally, prior to contacting the biological sample with the at least one first capture antibody or antigen, the at least one first capture antibody or antigen can be bound to a solid support which facilitates the separation the capture antibody / antigen-analyte-detection antibody / antigen complex from the biological sample. In some aspects, the biological sample is a concentrated biological sample. Any solid support known in the art can be used, including but not limited to, solid supports made out of polymeric materials in the forms of wells, tubes or beads. The antibody (or antibodies) / antigen (or antigens) can be bound to the solid support by adsorption, by covalent bonding using a chemical coupling agent or by other means known in the art, provided that such binding does not interfere with the ability of the antibody to bind the marker. Moreover, if necessary, the solid support can be derivatized to allow reactivity with various functional groups on the antibody or antigen. Such derivatization requires the use of certain coupling agents such as, but not limited to, maleic anhydride, N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. After the biological sample suspected of containing analyte brought into contact with the at least one first capture antibody or aptamer / antigen, the test sample is incubated to allow for the formation of a capture antibody or aptamer / antigen (or multiple antibody / multiple antigen)-analyte-at least one detection antibody or aptimer / antigen complex. The incubation can be carried out at a pH of from about 4.5 to about 10.0, at a temperature of from about 2° C to about 45° C, and for a period from 69Docket No. ABBTT-43596.601 at least about one (1) minute to about eighteen (18) hours, from about 2 to about 6 minutes, from about 3 to about 4 minutes, from about 10 to about 15 minutes, or about 18 to about 20 minutes. In some embodiments, when the concentration of an analyte is determined in a biological sample obtained from the subject, the concentration of the analyte is compared to a reference level. Specifically, a reference level of analyte can be employed as a benchmark against which to assess results obtained upon assaying a biological sample for analyte. Generally, in making such a comparison, the reference level of analyte, is obtained by running or conducting a particular assay a sufficient number of times and under appropriate conditions such that a linkage or association of analyte presence, amount or concentration with a particular stage or endpoint of HCC. Typically, the reference level of analyte is obtained with assays of reference subjects (or populations of subjects). 7. Digital microfluidics In certain embodiments, the methods described herein can be performed using digital microfluidics. For example, the methods described herein can be performed on or in one or more chips (e.g., lab-on-chip or biochip), cartridges, or combinations thereof. In certain embodiments, the methods described herein are performed using a digital microfluidics device (DMF). In certain embodiments, methods described herein are performed using a surface acoustic wave-based microfluidics device (SAW). In other embodiments, the methods described herein are performed using a micro-total analysis system or a biomedical (or biological) microelectromechanical system (BIOMEMs). In still other embodiments, the methods described herein are performed using an integrated DMF and analyte detection device. In certain embodiments, methods described herein are performed using an integrated surface acoustic wave based microfluidic device and analyte detection device. In certain embodiments, methods described herein are performed using a robotics-based assay processing unit. For example, in some embodiments, methods such as those described in U.S. Patent Application Publication No.2018 / 0095067, the contents of which are herein incorporated by reference, can be used. Specifically, in some embodiments, the methods involve measuring an analyte of interest in a liquid droplet (wherein the analyte of interest is from a test or biological sample). The method includes providing a first liquid droplet containing an analyte of interest, providing a second liquid droplet containing at least one solid support (such as, for example, a magnetic solid support (such as a bead or microparticle)) which contains a specific binding member that binds to the analyte of interest, using energy to exert a force to manipulate the first liquid droplet (which contains the analyte of interest) with the second liquid (containing the at least one solid support) to create a mixture, moving all or at least a portion of the mixture to an array of wells (where one or more wells of the array are of sufficient size to accommodate the at least one solid support), adding at least one detectable label to the mixture before, after or both before or after moving a portion of the mixture to the array of wells and measuring the analyte of interest in the wells. 70Docket No. ABBTT-43596.601 In certain embodiments, the detectable label comprises a color code comprising a specific dye coded to detect a specific analyte of interest (e.g., using a detection agent described herein to detect an antigen of interest). In certain embodiments, “using energy to exert a force to manipulate the first liquid droplet with the second liquid droplet” refers to the use of non-mechanical forces (namely, for example, energy created without the use of pumps and / or valves) to provide or exert a force that manipulates (such as merges or combines) at least the first and second liquid droplets (and optionally, additional droplets) into a mixture. Example of non-mechanical forces that can be used in the methods described herein include electric actuation force (such as droplet actuation, electrophoresis, electrowetting, dielectrophoresis, electrostatic actuation, electric field mediated, electrode mediated, capillary force, chromatography, centrifugation or aspiration) and / or acoustic force (such as surface acoustic wave (or “SAW”). In certain embodiments, the electric actuation force generated is an alternating current. For example, the alternating current can have a root mean squared (rms) voltage of 10 V, 15 V, 20 V, 25 V, 30 V, 35V or more. For example, such alternating current can have a rms voltage of 10 V or more, 15 V or more, 20 V or more, 25 V or more, 30 V or more or 35 V or more. Alternatively, the alternating current can have a frequency in a radio frequency range. In certain embodiments, if magnetic solid supports are used, an electric actuation force and a magnetic field can be applied and applied from opposition directions, relative to the at least a portion of the mixture. In certain other embodiments, the mixture is mixed by moving it: back and forth, in a circular pattern or by splitting it into two or more sub-mixtures and then merging the sub- mixtures. In certain other embodiments, an electric actuation force can be generated using a series or plurality of electrodes (namely, at least two or more, at least three or more, at least four or more, at least five or more, at least six or more, at least seven or more, at least eight or more, at least nine or more, at least ten or more, at least eleven or more, at least twelve or more, at least thirteen or more, at least fourteen or more, at least fifteen or more, etc.) to move the mixture to the array of wells in order to seal the wells (which are loaded with at least one solid support). In certain embodiments, the moving of all or at least a portion of the mixture to an array of wells results in the loading (filling and / or placement) of the at least one solid support into the array of reactions vessels (e.g., wells). In certain embodiments, when wells are used, a magnetic field is used to facilitate movement of the mixture and thus, at least one solid support, into one or more wells of the array. In certain embodiments, after the at least one solid support is loaded into the wells, any solid supports that are not loaded into a well can be removed using routine techniques known in the art. For example, such removing can involve generating an electric actuation force (such as that described previously herein) with a series or plurality of electrodes to move a fluid droplet (such as a polarizable fluid droplet) to the array of wells to move at least a portion of the mixture to a distance (the length of which is not critical) from the array of wells. In certain embodiments, an aqueous washing liquid can 71Docket No. ABBTT-43596.601 be used to remove the solid supports not bound to any analyte of interest. In such embodiments, the removal involves generating an electric actuation force with a series or plurality of electrodes to move an aqueous wash (or washing) droplet (a third droplet) across the array of wells. The amount and type of aqueous liquid used for said washing is not critical. In certain embodiments, the mixture in the method is an aqueous liquid. In other embodiments, the mixture is an immiscible liquid. In other embodiments, the liquid droplet is a hydrophobic liquid droplet. In other embodiments, the liquid droplet is a hydrophilic liquid droplet. In certain embodiments, the array of wells used in the method have a hydrophobic surface. In other embodiments, the array of wells has a hydrophilic surface. In certain embodiments, the first liquid droplet used in the method is a polarizable liquid. In certain embodiments, the second liquid droplet used in the method is a polarizable liquid. In certain embodiments, the first and second liquid droplets used in the method are polarizable liquids. In certain embodiments, the mixture is a polarizable liquid. In certain embodiments one or more of the first droplet, second droplet and mixture is a polarizable liquid. In certain embodiments, the at least one solid support comprises at least one binding member that specifically binds to the analyte of interest. In certain embodiments, the detectable label is added to the mixture before moving at least a portion of the mixture to the array of wells. In some embodiments, the detectable label comprises a color-coded dye that is specific for detecting the analyte of interest (e.g., antigen). In certain other embodiments, the detectable label is added to the mixture after the moving of at least a portion of the analyte of interest to the array of wells. In certain embodiments, the method further comprises positioning the at least a portion of the mixture over the array of wells, using a capillary element configured to facilitate movement of the mixture to the array of wells. In certain embodiments, the measuring first involves determining the total number of solid supports in a well, of the array (“total solid support number”). Next, the number of solid supports in the wells of the array that contain the detectable label are determined, such as, for example, determining the intensity of the signal produced by the detectable label (“positives”). The positives are subtracted from the total solid support number to provide the number of solid supports in the array of wells that do not contain a detectable label or are not detected (“negatives”). Then, the ratio of positives to negatives in the array of wells can be determined and then compared to a calibration curve. Alternatively, digital quantitation using the Poission equation P(x; μ) as shown below: P(x;μ)=(e−μ)(μx) / x! where: e: A is a constant equal to approximately 2.71828, μ: means number of successes that occur in a specified region, and x: is the tactual number of successes that occur in a specified region. 72Docket No. ABBTT-43596.601 Provided herein are methods for measuring or detecting an analyte present in a biological sample. The method includes contacting the sample with a first binding member, wherein the first binding member is immobilized on a solid support and wherein the first binding member specifically binds to the analyte; contacting the analyte with a second binding member, wherein the second binding member specifically binds to the analyte and wherein the second binding member includes a cleavable detectable label (or tag) attached thereto; removing second binding member not bound to the analyte bound to the first binding member; cleaving the detectable label attached to the second binding member that is bound to the analyte bound to the first binding member; translocating the cleaved detectable label through or across one or more nanopores in a layer; detecting or measuring detectable labels translocating through the layer; and assessing the detectable labels translocating through the layer, wherein measuring the number of detectable labels translocating through the layer measures the amount of analyte present in the sample, or wherein detecting labels translocating through the layer detects that the analyte is present in the sample. In some embodiments, measuring the labels translocating through the layer is assessed, wherein the number of labels translocating through the layer measures the amount of analyte present in the sample. In some embodiments, detecting the labels translocating through the layer is assessed, wherein detecting labels translocating through the layer detects that the analyte is present in the sample. In still yet other embodiments, each detectable label (e.g., different colored dye or combination of coded labels or dyes) or tag, such as an aptamer, translocating through the layer is a translocation event. Measuring the number of translocation events measures the amount of analyte present in the sample. In some embodiments, the amount of analyte present in the sample can be determined by counting the number of translocation events during a set period of time and correlating the number of translocation events to a control. The standard curve can be determined by measuring the number of translocation events for control concentrations of analyte during a set period of time. In some embodiments, the amount of analyte present in the sample can be determined by measuring the amount of time for a set number of translocation events to occur and correlating to a control. The standard curve can be determined by measuring the time it takes for a set number of translocation events to occur for control concentrations of analyte. In some embodiments, the amount of analyte present in the sample can be determined by measuring the average time between translocation events to occur and correlating to a control. The standard curve can be determined by measuring the average time between translocation events to occur for control concentrations of analyte. In some embodiments, the control can be a reference standard comprising a calibration curve, standard addition, or digital polymerase chain reaction. For example, in some embodiments, the methods may include contacting the sample with a first binding member (“binding members” also referred to herein as “specific binding members,”), where the first binding member is immobilized on a solid support and where the first binding member 73Docket No. ABBTT-43596.601 specifically binds to the analyte; contacting the analyte with a second binding member, which second binding member specifically binds to the analyte and which second binding member includes a cleavable detectable label attached thereto; removing second binding member not bound to the analyte bound to the first binding member; cleaving the detectable labels attached to the second binding member that is bound to the analyte bound to the first binding member; translocating the detectable label through nanopores in a layer; determining the number of detectable labels translocating through the layer; determining concentration of the analyte in the sample based on the number of detectable labels translocating through the layer. In certain embodiments, the concentration of the analyte may be determined by counting the number of detectable labels translocating through the layer per unit time. In other embodiments, the concentration of the analyte may be determined by determining the time at which the number of detectable labels translocating through the layer reaches a threshold. In certain embodiments, the methods of the present disclosure are carried out using a device in which a digital microfluidics module is integrated with an analyte detection device, such as those known in the art. In certain embodiments, the digital integrated microfluidics module and the analyte detection device may be reversibly integrated. For example, the two modules may be combined physically to form the integrated device and which device could then be separated into the individual modules. In certain embodiments, the methods of the present disclosure are carried out using a disposable cartridge that includes a microfluidics module with a built-in analyte detection device. The moving of the droplets in the integrated microfluidic and analyte detection device may be carried out using electrical force (e.g., electrowetting, dielectrophoresis, electrode-mediated, opto- electrowetting, electric-field mediated, and electrostatic actuation) pressure, surface acoustic waves and the like. The force used for moving the droplets may be determined based on the specifics of the device using techniques known in the art. For example, in some embodiments, the methods include generating a droplet of the sample and combining the droplet of the sample with a droplet containing the first binding member to generate a single droplet. The first binding member may be immobilized on a solid substrate, such as, a bead (e.g., a magnetic bead). The single droplet may be incubated for a time sufficient to allow binding of the first binding member to an analyte present in the sample droplet. Optionally, the single droplet may be agitated to facilitate mixing of the sample with the first binding member. Mixing may be achieved by moving the single droplet back and forth, moving the single droplet around over a plurality of electrodes, splitting a droplet and then merging the droplets, or using SAWs, and the like. Next, the single droplet may be subjected to a magnetic force to retain the beads at a location in the device while the droplet may be moved away and replaced with a droplet containing a second binding member. An optional wash step may be performed, prior to adding the second binding member, by moving a droplet of wash buffer to the location at which the beads are retained using the magnetic 74Docket No. ABBTT-43596.601 force. After a period of time sufficient for the second binding member to bind the analyte bound to the first binding member, the droplet containing the second binding member may be moved away while the beads are retained at the first location. The beads may be washed using a droplet of wash buffer followed by contacting the beads with a droplet containing a cleavage reagent to cleave the tag attached to the second binding member. In embodiments where the tag is attached to the second binding member via a photocleavable linker, the beads may be exposed to light of the appropriate wavelength to cleave the linker. In certain cases, the beads may be exposed to a droplet of buffer prior to cleavage of the photocleavable linker. Optionally, after the washing step to remove any unbound second binding member, a droplet containing buffer may be left covering the beads, the magnetic force retaining the beads at the first location may be removed and the buffer droplet containing the beads may be moved to a second location at which the photocleavage may be carried out. The droplet containing the cleaved tags may then be moved to the nanopore device or the nanopore module portion of the integrated device. In embodiments using aptamer as the second binding member, after the washing step to remove any unbound aptamer, a droplet containing buffer may be left covering the beads, the magnetic force retaining the beads at the first location may be removed and the buffer droplet containing the beads may be moved to a second location at which the dissociation of the aptamer may be carried out. In other embodiments, after the washing step, the beads may be exposed to a droplet of a reagent for dissociating aptamer bound to the analyte. A droplet containing the dissociated aptamer may be moved to the nanopore while the beads may be retained in place using a magnet. The droplet containing the dissociated aptamer may be moved to the nanopore device or the nanopore module portion of the integrated device. In some embodiments, the first binding member may be immobilized on a surface of the first or the second substrate at a location in the gap / space. The step of contacting a sample with the first binding member may include moving a droplet of the sample to the location in the gap / space at which the first binding member is immobilized. The subsequent steps may be substantially similar to those described above for first binding member immobilized on magnetic beads. After the cleaving / dissociating step, the droplet containing the cleaved tag(s) / dissociated aptamer(s) may be moved to the nanopore device or the nanopore module of the integrated device. As noted above, the droplet(s) may be moved using a liquid transfer system, such as a pipette. In certain cases, the microfluidic module may be fluidically connected to the nanopore module. Fluidic connection may be achieved by connecting the microfluidics module to the nanopore module via a channel or by placing the nanopore module within the microfluidics module, either reversibly or during the manufacturing process of the integrated device. Such devices are further described in the following section. In the above embodiments, optionally, after the combining, a droplet may be manipulated (e.g., moved back and forth, moved in a circular direction, oscillated, split / merged, exposed to SAW, 75Docket No. ABBTT-43596.601 etc.) to facilitate mixing of the sample with the assay reagents, such as, the first binding member, second binding member, etc. The moving of the droplets in the integrated microfluidics nanopore device may be carried out using electrical force (e.g., electrowetting, dielectrophoresis, electrode-mediated, opto- electrowetting, electric-field mediated, and electrostatic actuation) pressure, surface acoustic waves and the like. The force used for moving the droplets may be determined based on the specifics of the device and can be determined using techniques known in the art. 8. High-throughput and automated screening The present disclosure contemplates the use of high-throughput and automated screening processes. It is desirable in a variety of applications to measure and analyze the behavior of a large number of tissue and / or cell samples (e.g., dozens, hundreds, or thousands of samples representing variation across a variety of experimental conditions). Such analysis can facilitate the assessment of a variety of therapeutic treatments, environmental conditions, genetic modifications, communicable diseases, or other specified conditions to assess the efficacy of a treatment, to determine the toxicity of a substance, or to determine some other information of interest. For example, such high-throughput screening of hundreds or thousands of different samples can facilitate the identification of high- growth clone cell lines, while reducing the cost and / or time associated with such processes. This detection and analysis can include extracting small volumes from each sample to perform flow cytometry and generating cell counts or other information about the population(s) of cells in each sample, such as cell health and consistency of the sample. A robotic sample handling apparatus can be used to reduce the cost and / or time required to perform such assessments on many samples. Such “high throughput screening” apparatus and methods can include using multi-well sample plates or other multi-sample containers that permit robots or other apparatus to interact with dozens, hundreds, or thousands of samples in a single container in an automated manner. This can include placing a multi-well sample plate into an automated flow cytometry instrument that includes a robotic sample extraction head. Such a sample extraction head can move in two or more dimensions, facilitating the extraction of fluid from samples across the multi-well sample plate, as well as extracting chemical reagents, flushing of cleaning fluids, or other substances from reservoirs located nearby. The high throughput screening, volumetric flow cytometry systems, are examples of such a robotic sample handling apparatus that can provide fast, low-cost screening of samples. An additional robotic arm or other automated sample handling apparatus can also be used to present multiple different multi-well sample plates to the flow cytometry instrument over time, facilitating the automated assessment of even more samples with no or minimal human intervention. Such a system can operate to screen very many samples over an extended period of time without 76Docket No. ABBTT-43596.601 human intervention, e.g., for longer than 48 hours. Such a combined system can include expanded reservoirs for rinse fluids, cleaning fluids, or other consumables. Additionally or alternatively, local reservoirs that are accessible by a sample head of a robotic sample handling apparatus can be augmented by additional remote reservoirs that are operable to automatically replenish the local reservoirs. 9. Samples Aspects of the disclosure involve detection of analytes of interest in samples. The disclosure is not limited to the type of sample in which an analyte can be detected. In some instances, the sample is diluted. In other instances, the sample is undiluted. In some aspects, the sample is obtained from a subject (e.g., human subject) and comprises a whole blood sample. In other aspects, the sample comprises a plasma sample. In other aspects, the sample comprises a serum sample. In other aspects, the sample comprises a urine sample. In still other aspects, the sample comprises a cerebrospinal fluid sample. In some aspects, the sample comprises or is suspected to comprise one or more nucleic acids capable of analysis by the methods. Preferably, the samples comprise nucleic acids (e.g., DNA, RNA, cDNAs, microRNA, mitochondrial DNA, etc.). Samples may be complex samples or mixed samples, which contain nucleic acids comprising multiple different nucleic acid sequences (e.g. host and pathogen nucleic acids; mutant and wild-type species; heterogeneous tumor. Samples may comprise nucleic acids from more than one source (e.g. different species, different subspecies, etc.), subject, and / or individual. In some aspects, the sample obtained from a subject (e.g., a human subject) comprises a venous blood sample, such as, whole blood, serum or plasma. In other aspects, the sample obtained from a subject (e.g., a human subject) is a capillary blood sample, such as, whole blood, serum or plasma. In some aspects, the capillary blood sample is whole blood. In other aspects, the capillary blood sample is serum. In yet other aspects, the capillary blood sample is plasma. The capillary blood sample can be obtained by extracting the sample through the skin (e.g., such as the fingers and / or toes, a hand, a foot (including the heel), an earlobe, a location on the arms and / or legs, chest, back, head, or any combinations thereof) of a subject. In some aspects, the capillary blood sample is extracted from the fingers or toes. In other aspects, the whole capillary blood sample is extracted from the arms or legs. In still other aspects, the capillary blood sample is obtained from the hands or feet. In still other aspects, the capillary blood sample is obtained from the chest or back. In yet other aspects, the capillary blood sample is obtained from an earlobe. In still other aspects, the capillary blood sample is obtained from the head. In some aspects, the capillary blood sample obtained from the subject is obtained without the use of a syringe, needle (e.g., 21-gauge needle, a butterfly needle, etc.), or any other suitable device, or any combination thereof which are typically used to draw blood (e.g., venous blood). 77Docket No. ABBTT-43596.601 Instead, the capillary blood sample is obtained using a self- or other-administered blood collection device. Examples of self-or other-administered blood collection devices include microsampling devices. Example microsampling devices which can be used herein include the TAP device available from YourBio Health, Inc. (Cambridge, MA) as well as the device described in U.S. Patent No. 9,113,836, the contents of which are herein incorporated by reference, the Tasso+, Tasso-M20, and Tasso-ST devices available from Tasso, Inc. (Seattle, WA), the One Draw device available from Draw Bridge Health (San Diego, CA), PBS-1000 from PreciHealth (Neuchatel, Switzerland) or the Loop blood collection device available from Loop Medical (Lausanne, Switzerland). In other aspects, the capillary blood sample is obtained or collected from a subject in a decentralized setting. For example, the capillary blood sample can be obtained or collected from an urgent care clinic, a pharmacy, a grocery or other convenience store, a residence, a workplace, and / or a government office. In addition or alternatively, in still yet further aspects, the capillary blood sample is obtained from the subject by a user who is not trained in collecting blood (e.g., by someone other than a trained phlebotomist, a nurse, a medical assistant and / or physician). For example, the capillary blood sample can be obtained by the subject, a relative, friend, a co-worker, a coach, a pharmacist, and / or any other individual. In still yet further aspects, the capillary blood sample is obtained from a subject by a robot. In some aspects, the capillary blood sample or venous blood sample obtained from the subject is in an amount of less than about 4 mL. In some aspects, the capillary blood or venous blood sample obtained from the subject is less than about 3 mL. In some aspects, the capillary or venous blood sample obtained from the subject is less than about 2 mL. In some aspects, the amount of capillary or venous blood sample obtained from the subject is less than about 3.9 mL, about 3.8 mL, about 3.7 mL, about 3.6 mL, about 3.5 mL, about 3.4 mL, about 3.3 mL, about 3.2 mL, about 3.1 mL, about 3.0 mL, about 2.9 mL, about 2.8 mL, about 2.7 mL, about 2.6 mL, about 2.5 mL, about 2.4 mL, about 2.3 mL, about 2.2 mL, about 2.1 mL, about 2.0 ml, about 1.9 mL, about 1.8 mL, about 1.7 mL, about 1.6 mL, about 1.5 mL, about 1.4 mL, about 1.3 mL, about 1.2 mL, about 1.1 mL, about 1.0 mL, about 0.9 mL, about 0.8 mL, about 0.7 mL, about 0.6 mL, or about 0.5 mL. In some aspects, higher volumes of capillary or venous blood may be obtained when the sample collected is whole blood. In some aspects, the sample (e.g., capillary or venous blood sample) obtained from a subject is whole blood that is subjected to further or additional processing prior to determining the amount of an analyte (e.g., biomarker) in the sample. In some aspects, the sample is processed using centrifugation. In yet other aspects, the sample is processed using a plasma separation device which may comprise at least one filter, membrane and / or synthetic paper. The plasma separation device can separate whole blood into serum and / or plasma which can then be used in the methods described herein. 78Docket No. ABBTT-43596.601 In other aspects, the plasma separation device can be in fluid communication with or operably linked, coupled and / or removably coupled to a microsampling device as part of a system. In other aspects, the plasma separation device can be integrated into the microsampling device. In still yet other aspects, the plasma separation device can be in fluid communication with or operably linked, coupled and / or removably coupled to an aperture of a reaction vessel. For example, in some aspects, the reaction vessel is a cartridge such as those used in a point-of-care device. In still other aspects, the plasma separation device can be in fluid communication with or operably linked, coupled and / or removably coupled to a transfer tube. In these aspects, the transfer can be in fluid communication with or operably linked, coupled and / or removably coupled to a reaction vessel. In still further aspects, the plasma separation device can be integrated into the transfer tube. In yet other aspects, the transfer tube includes a cap or a stopper. Examples of assays that can be used to determine the amount of an analyte (e.g., biomarker) of interest in the sample include, without limitation, an immunoassay, such as an enzyme immunoassay (EIA), an enzyme linked immunosorbent assay (ELISA), a fluorescent immunoassay, a chemiluminescence Immunoassay (CLIA), a radioimmunoassay (RIA), a microparticle enzyme immunoassay (MEIA), a turbidimetric immunoassay, etc. In yet other aspects, the assay may be a clinical chemistry assay such as, for example, a photometry, a spectrophotometry, an absorbance, a fluorescence, a turbidimetry, a nephelometry, a potentiometry and / or an electrophoresis assay. In yet further aspects, the assay may be a combination of an immunoassay and a clinical chemistry assay. In still other aspects, the assay may be a single molecule detection assay. The assays may be performed sequentially, in any order. Generally, a reference level of the analyte (e.g., biomarker) can also be employed as a benchmark against which to assess results obtained upon assaying a test sample for the biomarker. Generally, in making such a comparison, the reference level of the biomarker is obtained by running or conducting a particular assay a sufficient number of times and under appropriate conditions such that a linkage or association of analyte presence, amount or concentration with a particular stage or endpoint of an illness, disease and / or condition or with particular indicia can be made. Typically, the reference level of the biomarker is obtained with assays of reference subjects (or populations of subjects). The biomarker measured can include fragments thereof, degradation products thereof, and / or enzymatic cleavage products thereof. In certain aspects, the reference level may be correlated with control subjects (e.g., human subjects) that do not have the illness, condition, or disorder. In yet further aspects, once the amount of analyte (e.g., biomarker) is determined using the methods described herein, a result is obtained. This result can be further processed. Specifically, this further processing can involve selecting a conversion factor for comparing the amount of analyte (e.g., 79Docket No. ABBTT-43596.601 biomarker) in the capillary whole blood or plasma sample with the amount of the same analyte in venous whole blood or plasma. Once the conversion factor is selected, the processing further involves normalizing the amount of analyte in the capillary whole blood or plasma sample with the amount of the same analyte from venous whole blood or plasma by applying the conversion factor to the amount of analyte in the sample. For example, the amount of the analyte in the capillary whole blood or plasma sample can be multiplied by the conversion factor to provide the normalized amount of the analyte in the sample. In some aspects, the processing of the amount of the analyte (e.g., biomarker) can be by a processing system which comprises a computer processor and a non-transitory computer memory comprising one or more computer programs. In some embodiments, when a capillary sample is used, the one or more computer programs can be used in conjunction with said computer process to select a conversion factor for comparing the amount of the biomarker in the capillary whole blood or plasma sample with the amount of the same analyte in venous whole blood or plasma and normalize the amount of analyte in the sample with the amount of the same analyte from venous whole blood or plasma by applying the selected conversion factor to the amount analyte in the sample. This result or processed result can be communicated (e.g., reported) for further analysis, interpretation, processing and / or display. The result can be communicated (e.g., reported) by a computer, in a document and / or spreadsheet, on a mobile device (e.g., a smart phone), on a website, in an e-mail, or any combination thereof. In some aspects, the result can displayed not as a number, but as a visual signal (e.g., a line or bar) that can be read / interpreted by a reader or the naked eye. In some aspects, the result determined in the sample is communicated by being displayed, such as on an instrument. In further aspects, the result is displayed as indicating that the amount of analyte in a subject is elevated, are not elevated, or that the assay(s) for the analyte should be repeated. Suitable instruments for use in the methods described herein include a higher throughput assay analyzer (e.g., the ARCHITECT platform marketed by Abbott Laboratories) or a point-of-care device (e.g., i-STAT and i-STAT Alinity devices marketed by Abbott Laboratories) that may contain a user interface that can display the determination. In some aspects, the instrument contains software to execute one or more tasks, including the performance of the methods and algorithms described herein. In some aspects, the instrument contains software to automatically determine the next appropriate step in a methods and algorithms as described herein. For example, the instrument may contain software that determines the amount or presence of an analyte of interest. The software may display this determination, such as on a graphical user interface. In some aspects, the instrument stores software that instructs a processor to execute a given task. In some aspects, the software stores machine readable instructions that instruct a processor to 80Docket No. ABBTT-43596.601 execute a given task. The machine-readable instructions may be one or more executable programs or portion(s) of an executable program for execution by a computer. The programs may be embodied in software stored on a non-transitory computer readable storage medium such as a CD-ROM, a floppy disk, a hard drive, a DVD, a Blu-ray disk, or a memory associated with the processors. Alternatively, the entire programs and / or parts thereof could alternatively be executed by a device other than the processors and / or embodied in firmware or dedicated hardware. Additionally or alternatively, processes may be implemented by one or more hardware circuits (e.g., discrete and / or integrated analog and / or digital circuitry, an FPGA, an ASIC, a comparator, an operational-amplifier (op-amp), a logic circuit, etc.) structured to perform the corresponding operation without executing software or firmware. The machine-readable instructions may be stored in one or more of a compressed format, an encrypted format, a fragmented format, a compiled format, an executable format, a packaged format, etc. Machine readable instructions as described herein may be stored as data (e.g., portions of instructions, code, representations of code, etc.) that may be utilized to create, manufacture, and / or produce machine executable instructions. For example, the machine-readable instructions may be fragmented and stored on one or more storage devices and / or computing devices (e.g., servers). The machine-readable instructions may require one or more of installation, modification, adaptation, updating, combining, supplementing, configuring, decryption, decompression, unpacking, distribution, reassignment, compilation, etc. in order to make them directly readable, interpretable, and / or executable by a computing device and / or other machine. For example, the machine-readable instructions may be stored in multiple parts, which are individually compressed, encrypted, and stored on separate computing devices, wherein the parts when decrypted, decompressed, and combined form a set of executable instructions that implement a program such as that described herein. In another example, the machine-readable instructions may be stored in a state in which they may be read by a computer, but require addition of a library (e.g., a dynamic link library (DLL)), a software development kit (SDK), an application programming interface (API), etc. in order to execute the instructions on a particular computing device or other device. In another example, the machine-readable instructions may need to be configured (e.g., settings stored, data input, network addresses recorded, etc.) before the machine-readable instructions and / or the corresponding program(s) can be executed in whole or in part. Thus, the disclosed machine-readable instructions and / or corresponding program(s) are intended to encompass such machine-readable instructions and / or program(s) regardless of the particular format or state of the machine-readable instructions and / or program(s) when stored or otherwise at rest or in transit. The machine-readable instructions described herein can be represented by any past, present, or future instruction language, scripting language, programming language, etc. For example, the machine-readable instructions may be represented using any of the following languages: C, C++, 81Docket No. ABBTT-43596.601 Java, C#, Perl, Python, JavaScript, HyperText Markup Language (HTML), Structured Query Language (SQL), Swift, etc. The machine readable instructions may be stored on a non-transitory computer and / or machine readable medium such as a hard disk drive, a flash memory, a read-only memory, a compact disk, a digital versatile disk, a cache, a random-access memory and / or any other storage device or storage disk in which information is stored for any duration (e.g., for extended time periods, permanently, for brief instances, for temporarily buffering, and / or for caching of the information). As used herein, the term non-transitory computer readable medium is expressly defined to include any type of computer readable storage device and / or storage disk and to exclude propagating signals and to exclude transmission media. It may be desirable to include a control sample. The control sample may be analyzed concurrently with the sample from the subject as described above. The results obtained from the subject sample can be compared to the results obtained from the control sample. Standard curves may be provided, with which assay results for the sample may be compared. Such standard curves present levels of marker as a function of assay units, i.e., fluorescent signal intensity, if a fluorescent label is used. Using samples taken from multiple donors, standard curves can be provided for reference levels of an analyte (e.g., biomarker) in normal healthy subjects or tissue, as well as for “at-risk” levels of the analytes in tissue taken from donors, who may have one or more clinically relevant characteristics. 10. Methods for measuring the level of analyte of interest or biomarker In the methods described above, an analyte of interest or biomarker levels can be measured by any means, such as antibody dependent methods, such as immunoassays, protein immunoprecipitation, immunoelectrophoresis, chemical analysis, SDS-PAGE and Western blot analysis, or protein immunostaining, electrophoresis analysis, a protein assay, a competitive binding assay, a functional protein assay, or chromatography or spectrometry methods, such as high- performance liquid chromatography (HPLC) or liquid chromatography–mass spectrometry (LC / MS). Also, the assay can be employed in clinical chemistry format such as would be known by one skilled in the art. In some embodiments, the sample is diluted or undiluted. The sample can be from about 1 to about 25 microliters, about 1 to about 24 microliters, about 1 to about 23 microliters, about 1 to about 22 microliters, about 1 to about 21 microliters, about 1 to about 20 microliters, about 1 to about 18 microliters, about 1 to about 17 microliters, about 1 to about 16 microliters, about 15 microliters or about 1 microliter, about 2 microliters, about 3 microliters, about 4 microliters, about 5 microliters, about 6 microliters, about 7 microliters, about 8 microliters, about 9 microliters, about 10 microliters, about 11 microliters, about 12 microliters, about 13 microliters, about 14 microliters, about 15 microliters, about 16 microliters, about 17 microliters, about 18 microliters, about 19 microliters, 82Docket No. ABBTT-43596.601 about 20 microliters, about 21 microliters, about 22 microliters, about 23 microliters, about 24 microliters or about 25 microliters. In some embodiments, the sample is from about 1 to about 150 microliters or less or from about 1 to about 25 microliters or less. Other methods of detection include the use of or can be adapted for use on a nanopore device or nanowell device. Examples of nanopore devices are described in International Patent Publication No. WO 2016 / 161402, which is hereby incorporated by reference in its entirety. Examples of nanowell device are described in International Patent Publication No. WO 2016 / 161400, which is hereby incorporated by reference in its entirety 11. Variations on methods The methods may also be adapted in view of other methods for analyzing analytes. Examples of well-known variations include, but are not limited to, immunoassay, such as sandwich immunoassay (e.g., monoclonal-monoclonal sandwich immunoassays, monoclonal-polyclonal sandwich immunoassays, including enzyme detection (enzyme immunoassay (EIA) or enzyme-linked immunosorbent assay (ELISA), competitive inhibition immunoassay (e.g., forward and reverse), enzyme multiplied immunoassay technique (EMIT), a competitive binding assay, bioluminescence resonance energy transfer (BRET), one-step antibody detection assay, homogeneous assay, heterogeneous assay, capture on the fly assay, etc. The presence or amount of analyte (e.g., biomarker) present in a body sample may be readily determined using an immunoassay, such as sandwich immunoassay (e.g., monoclonal- monoclonal sandwich immunoassays, monoclonal-polyclonal sandwich immunoassays, including radioisotope detection (radioimmunoassay (RIA)) and enzyme detection (enzyme immunoassay (EIA) or enzyme-linked immunosorbent assay (ELISA) (e.g., Quantikine ELISA assays, R&D Systems, Minneapolis, MN)). An example of a point-of-care device that can be used is i-STAT® (Abbott, Laboratories, Abbott Park, IL). Other methods that can be used include a chemiluminescent microparticle immunoassay, in particular one employing the ARCHITECT® automated analyzer (Abbott Laboratories, Abbott Park, IL), as an example. Other methods include, for example, mass spectrometry, and immunohistochemistry (e.g., with sections from tissue biopsies), using anti-analyte antibodies (monoclonal, polyclonal, chimeric, humanized, human, etc.) or antibody fragments thereof against analyte. Other methods of detection include those described in, for example, U.S. Patent Nos. 6,143,576; 6,113,855; 6,019,944; 5,985,579; 5,947,124; 5,939,272; 5,922,615; 5,885,527; 5,851,776; 5,824,799; 5,679,526; 5,525,524; and 5,480,792, each of which is hereby incorporated by reference in its entirety. Specific immunological binding of the antibody to the analyte can be detected via direct labels, such as fluorescent or luminescent tags, metals and radionuclides attached to the antibody or via indirect labels, such as alkaline phosphatase or horseradish peroxidase. The use of immobilized antibodies or antibody fragments thereof may be incorporated into the immunoassay. The antibodies may be immobilized onto a variety of supports, such as magnetic or 83Docket No. ABBTT-43596.601 chromatographic matrix particles, the surface of an assay plate (such as microtiter wells), pieces of a solid substrate material, and the like. An assay strip can be prepared by coating the antibody or plurality of antibodies in an array on a solid support. Any solid support known in the art can be used, including but not limited to, solid supports made from polymeric materials in the forms of wells, tubes or beads. This strip can then be dipped into the test sample and processed quickly through washes and detection steps to generate a measurable signal, such as a colored spot. A homogeneous format may be used. For example, after the test sample is obtained from a subject, a mixture is prepared. The mixture contains the test sample being assessed for analyte (e.g., biomarker), a first specific binding partner, and a second specific binding partner. The order in which the test sample, the first specific binding partner, and the second specific binding partner are added to form the mixture is not critical. The test sample is simultaneously contacted with the first specific binding partner and the second specific binding partner. In some embodiments, the first specific binding partner and any biomarker contained in the test sample may form a first specific binding partner-analyte-antigen complex and the second specific binding partner may form a first specific binding partner-analyte of interest (e.g., biomarker)-second specific binding partner complex. The first specific binding partner may be an anti-analyte antibody. The second specific binding partner may be an anti-analyte antibody. Moreover, the second specific binding partner is labeled with or contains a detectable label as described above. A heterogeneous format may be used. For example, after the test sample is obtained from a subject, a first mixture is prepared. The mixture contains the test sample being assessed for analyte (e.g., biomarker) and a first specific binding partner, wherein the first specific binding partner and any biomarker contained in the test sample form a first specific binding partner-analyte-antigen complex. The order in which the test sample and the first specific binding partner are added to form the mixture is not critical. In those embodiments where the solid phase is a bead, the bead may be a magnetic bead or a magnetic particle. Magnetic beads / particles may be ferromagnetic, ferrimagnetic, paramagnetic, superparamagnetic or ferrofluidic. Exemplary ferromagnetic materials include Fe, Co, Ni, Gd, Dy, CrO2, MnAs, MnBi, EuO, and NiO / Fe. Examples of ferrimagnetic materials include NiFe2O4, CoFe2O4, Fe3O4(or FeO.Fe2O3). Beads can have a solid core portion that is magnetic and is surrounded by one or more non-magnetic layers. Alternately, the magnetic portion can be a layer around a non-magnetic core. The beads on which the first specific binding member is immobilized may be stored in dry form or in a liquid. The magnetic beads may be subjected to a magnetic field prior to or after contacting with the sample with a magnetic bead on which the first specific binding member is immobilized. 12. Kits The systems and methods herein can be implemented using a kit of the disclosure. 84Docket No. ABBTT-43596.601 In an aspect, the disclosure provides kits for performing a digital multiplexed nucleic acid amplification assay to detect a plurality of target nucleic acids in a sample. The kit can include a plurality of nucleic acid probes that are labeled with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest. The kit can also include instruments for performing amplification assays and amplification reagents. In some instances, the kit includes a reaction vessel or plurality of reaction vessels. In some embodiments, the kit includes (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and / or (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye. The kit can also include instructions for labeling at least three different nucleic acid probes with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest. In some instances, the kit includes instructions for aliquoting a sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel contains a single molecule of target nucleic acid and the at least three different nucleic acid probes. The kit can also provide instructions for amplifying in each reaction vessel, on average, the single molecule of the target nucleic acid. The kit can also include instructions for detecting in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (iii) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel. In another embodiment, the disclosure provides a kit for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample. The kit can include at least at least three different detection agents labeled with at least two different color labels or dyes to create a set of color codes specific for at least three different target antigens of interest. In some embodiments, the kit includes (i) at least a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) at least a second detection agent specific for a second target antigen is labeled with a second color label or dye; and / or (iii) at least a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye. The kit can also include instruments or equipment for performing a digital immunoassay. 85Docket No. ABBTT-43596.601 In some embodiments, the kit includes instructions for labeling at least three different detection agents with at least two different color labels or dyes to create a set of color codes specific for at least three different target antigens of interest. The kit can also include instructions for performing a digital immunoassay in parallel for each of the at least three different target antigens to detect, on average, a single one of the at least three different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; and (iii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the third detection agent detects the third target antigen. The kit can also include a sample collection device (e.g., a microsampling device, a syringe, etc) and instructions for collecting a sample using the collection device. Provided herein is a kit, which may be used for assaying or assessing a test sample for an analyte of interest. The kit comprises at least one component for assaying the test sample for the analyte of interest, and instructions for assaying the test sample for the analyte of interest. For example, the kit can comprise instructions for assaying the test sample for an analyte of interest by immunoassay, e.g., chemiluminescent microparticle immunoassay. Instructions included in kits can be affixed to packaging material or can be included as a package insert. While the instructions are typically written or printed materials, they are not limited to such. Any medium capable of storing such instructions and communicating them to an end user is contemplated by this disclosure. Such media include, but are not limited to, electronic storage media (e.g., magnetic discs, tapes, cartridges, chips), optical media (e.g., CD ROM), and the like. As used herein, the term "instructions" can include the address of an internet site that provides the instructions. Alternatively or additionally, the kit can comprise a calibrator or control, e.g., purified, and optionally lyophilized, and / or at least one container (e.g., tube, microtiter plates or strips) for conducting the assay, and / or a buffer, such as an assay buffer or a wash buffer, either one of which can be provided as a concentrated solution, a substrate solution for the detectable label, or a stop solution. Preferably, the kit comprises all components, i.e., reagents, standards, buffers, diluents, etc., which are necessary to perform the assay. The instructions also can include instructions for generating a standard curve. Moreover, the kit can further comprise a microsampling device, a plasma separation device and / or a microsampling device and a plasma separation device. Optionally, the kit includes quality control components (for example, sensitivity panels, calibrators, and positive controls). Preparation of quality control reagents is well-known in the art and is described on insert sheets for a variety of immunodiagnostic products. Sensitivity panel members 86Docket No. ABBTT-43596.601 optionally are used to establish assay performance characteristics, and further optionally are useful indicators of the integrity of the immunoassay kit reagents, and the standardization of assays, The kit can also optionally include other reagents required to conduct a diagnostic assay or facilitate quality control evaluations, such as buffers, salts, enzymes, enzyme co-factors, substrates, detection reagents, and the like. Other components, such as buffers and solutions for the isolation and / or treatment of a test sample (e.g., pretreatment reagents), also can be included in the kit. The kit can additionally include one or more other controls. One or more of the components of the kit can be lyophilized, in which case the kit can further comprise reagents suitable for the reconstitution of the lyophilized components. The various components of the kit optionally are provided in suitable containers as necessary, e.g., a microtiter plate. The kit can further include containers for holding or storing a sample (e.g., a container or cartridge for a urine, whole blood, plasma, or serum sample). Where appropriate, the kit optionally also can contain reaction vessels, mixing vessels, and other components that facilitate the preparation of reagents or the test sample. The kit can also include one or more instruments for assisting with obtaining a test sample, such as a syringe, pipette, forceps, measured spoon, or the like. If the detectable label is at least one acridinium compound, the kit can comprise at least one acridinium-9-carboxamide, at least one acridinium-9-carboxylate aryl ester, or any combination thereof. If the detectable label is at least one acridinium compound, the kit also can comprise a source of hydrogen peroxide, such as a buffer, solution, and / or at least one basic solution. If desired, the kit can contain a solid phase, such as a magnetic particle, bead, test tube, microtiter plate, cuvette, membrane, scaffolding molecule, film, filter paper, disc, or chip. If desired, the kit can further comprise one or more components, alone or in further combination with instructions, for assaying the test sample for another analyte, which can be a biomarker, such as a biomarker of traumatic brain injury or disorder. It will be readily apparent to those skilled in the art that other suitable modifications and adaptations of the methods of the present disclosure described herein are readily applicable and appreciable, and may be made using suitable equivalents without departing from the scope of the present disclosure or the aspects and embodiments disclosed herein. Having now described the present disclosure in detail, the same will be more clearly understood by reference to the following examples, which are merely intended only to illustrate some aspects and embodiments of the disclosure, and should not be viewed as limiting to the scope of the disclosure. The disclosures of all journal references, U.S. patents, and publications referred to herein are hereby incorporated by reference in their entireties. EXAMPLES Example 1 87Docket No. ABBTT-43596.601 Immunoassays using a non-digital multiplex format face significant challenges due to reliance on dye combinations for detecting protein antigens in capture assays. This method often encounters issues such as dye interference and limited multiplexing capability, complicating the accurate and efficient measurement of multiple proteins simultaneously (FIG.1A). To overcome these challenges, ratios of labels or dyes can be used to expand the coding space for multi-parallel digital detection of various target analytes in nanowells, droplets, or other digital reaction vessels. By labeling individual nucleic acid probes or protein binders with combinations of labels or dyes, a broader range of targets can be identified. Samples are then prepared for nucleic acid (DNA or RNA) or antigen (protein, metabolite) testing, aliquoted into reaction vessels, and analyzed digitally using techniques such as PCR, isothermal amplification, or sandwich immunoassays. The spectral footprint of the labels or dyes is used to identify targets, with codes representing the combinations of labels or dyes used. The number of each target is determined by counting the reaction vessels with specific dye codes. In a digital detection scheme, the separation into discrete partitions or reaction vessels, reduces the likelihood of multiple labels or dyes being present in any single vessel. This separation allows for the combination of multiple labels or dyes on a single probe, facilitating additional target detection. For instance, a yellow and blue dye can be used for two targets and then can be combined for a third target (FIG.1B). Because the targets are spread out into individual reaction vessels, it will be possible to see yellow vessels where only target one is detected, blue vessels where only target two is present, and green (yellow and blue) in vessels where only target three resides (FIG.1B). Additional labels or dyes can be added to expand the coding space further, potentially providing fifteen or more codes for digital PCR or isothermal amplification nucleic acid assays, or digital immunoassays (FIG. 1C). Similarly, the digital multi-parallel format can reveal quantitative differences in dye combinations (FIG. 1D). This method enhances the ability to simultaneously measure a wide range of targets with high precision. The present disclosure has multiple aspects, illustrated by the non-limiting examples described herein. It is understood that the foregoing detailed description and accompanying examples are merely illustrative and are not to be taken as limitations upon the scope of the disclosure, which is defined solely by the appended claims and their equivalents. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications, including without limitation those relating to the chemical structures, substituents, derivatives, intermediates, syntheses, compositions, formulations, or methods of use of the disclosure, may be made without departing from the spirit and scope thereof. For reasons of completeness, various aspects of the disclosure are set out in the following numbered clauses: 88Docket No. ABBTT-43596.601 Clause 1. A method for performing a digital multiplexed nucleic acid amplification assay to detect a plurality of target nucleic acids in a sample, comprising: (a) labeling at least three different nucleic acid probes with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) aliquoting the sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel contains a single molecule of target nucleic acid and the at least three different nucleic acid probes; (c) amplifying in each reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (iii) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel. Clause 2. The method of clause 1, wherein step (a) comprises labeling at least six different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least six different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye;(iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye;(iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of dyes comprising the first color label or dye and the third color label or dye; and (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye. Clause 3. The method of clause 2, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel;(iv) the reaction vessel emits a light signal corresponding to the 89Docket No. ABBTT-43596.601 combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the fifth target nucleic acid is detected in the reaction vessel; and (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the target nucleic acid is detected in the reaction vessel. Clause 4. The method of clause 1, wherein step (a) comprises labeling at least ten different nucleic acid probes with at least four different colored labels or dyes to create a set of color codes specific for at least ten different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (viii) an eight nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of dyes comprising the second color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; and (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye. Clause 5. The method of clause 4, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the sixth target 90Docket No. ABBTT-43596.601 nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; and (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel. Clause 6. The method of clause 1, wherein step (a) comprises labeling at least 15 different nucleic acid probes with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (ix) a ninth nucleic acid probe specific for an ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; and (xv) a fifteenth nucleic 91Docket No. ABBTT-43596.601 acid probe specific for a fifteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye. Clause 7. The method of clause 6, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected...

Claims

Docket No. ABBTT-43596.601 CLAIMS What is claimed is:

1. A method for performing a digital multiplexed nucleic acid amplification assay to detect a plurality of target nucleic acids in a sample, comprising: (a) labeling at least three different nucleic acid probes with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) aliquoting the sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel contains a single molecule of target nucleic acid and the at least three different nucleic acid probes; (c) amplifying in each reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (iii) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel.

2. The method of claim 1, wherein step (a) comprises labeling at least six different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least six different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; 105Docket No. ABBTT-43596.601 (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; and (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye.

3. The method of claim 2, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the fifth target nucleic acid is detected in the reaction vessel; and (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the target nucleic acid is detected in the reaction vessel.

4. The method of claim 1, wherein step (a) comprises labeling at least ten different nucleic acid probes with at least four different colored labels or dyes to create a set of color codes specific for at least ten different target nucleic acids of interest, wherein: 106Docket No. ABBTT-43596.601 (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (viii) an eight nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; and (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye.

5. The method of claim 4, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; 107Docket No. ABBTT-43596.601 (v) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; and (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel.

6. The method of claim 1, wherein step (a) comprises labeling at least 15 different nucleic acid probes with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; 108Docket No. ABBTT-43596.601 (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (ix) a ninth nucleic acid probe specific for an ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; and (xv) a fifteenth nucleic acid probe specific for a fifteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye.

7. The method of claim 6, wherein: (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; 109Docket No. ABBTT-43596.601 (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fifth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the tenth target nucleic acid is detected in the reaction vessel; (xi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the eleventh target nucleic acid is detected in the reaction vessel; (xii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fifth color label or dye when the twelfth target nucleic acid is detected in the reaction vessel; (xiii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the thirteenth target nucleic acid is detected in the reaction vessel; 110Docket No. ABBTT-43596.601 (xiv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fifth color label or dye when the fourteenth target nucleic acid is detected in the reaction vessel; and (xv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye when the fifteenth target nucleic acid is detected in the reaction vessel.

8. The method of claim 1, wherein step (a) comprises labeling at least 21 different nucleic acid probes with at least six different colored labels or dyes to create a set of color codes specific for at least 21 different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a fourth color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a fifth color label or dye; (vi) a sixth nucleic acid probe specific for a fifth target nucleic acid is labeled with a sixth color label or dye; (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (viii) an eighth nucleic acid probe specific for an eighth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (ix) a ninth nucleic acid probe specific for a ninth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (x) a tenth nucleic acid probe specific for a tenth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (xi) an eleventh nucleic acid probe specific for an eleventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the sixth color label or dye; 111Docket No. ABBTT-43596.601 (xii) a twelfth nucleic acid probe specific for a twelfth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xiii) a thirteenth nucleic acid probe specific for a thirteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xiv) a fourteenth nucleic acid probe specific for a fourteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xv) a fifteenth nucleic acid probe specific for a fifteenth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the sixth color label or dye; (xvi) a sixteenth nucleic acid probe specific for a sixteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xvii) a seventeenth nucleic acid probe specific for a seventeenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; (xviii) an eighteenth nucleic acid probe specific for an eighteenth target nucleic acid is labeled with a combination of labels or dyes comprising the third color label or dye and the sixth color label or dye; (xix) a nineteenth nucleic acid probe specific for a nineteenth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye; (xx) a twentieth nucleic acid probe specific for a twentieth target nucleic acid is labeled with a combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye; and (xxi) a twenty-first nucleic acid probe specific for a twenty-first target nucleic acid is labeled with a combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye.

9. The method of claim 8, wherein: 112Docket No. ABBTT-43596.601 (i) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (ii) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; (iii) the reaction vessel emits a light signal corresponding to the third color label or dye when the third target nucleic acid is detected in the reaction vessel; (iv) the reaction vessel emits a light signal corresponding to the fourth color label or dye when the fourth target nucleic acid is detected in the reaction vessel; (v) the reaction vessel emits a light signal corresponding to the fifth color label or dye when the fifth target nucleic acid is detected in the reaction vessel; (vi) the reaction vessel emits a light signal corresponding to the sixth color label or dye when the sixth target nucleic acid is detected in the reaction vessel; (vii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the second color label or dye when the seventh target nucleic acid is detected in the reaction vessel; (viii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the third color label or dye when the eighth target nucleic acid is detected in the reaction vessel; (ix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fourth color label or dye when the ninth target nucleic acid is detected in the reaction vessel; (x) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the fifth color label or dye when the tenth target nucleic acid is detected in the reaction vessel; (xi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the first color label or dye and the sixth color label or dye when the eleventh target nucleic acid is detected in the reaction vessel; (xii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the third color label or dye when the twelfth target nucleic acid is detected in the reaction vessel; 113Docket No. ABBTT-43596.601 (xiii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fourth color label or dye when the thirteenth target nucleic acid is detected in the reaction vessel; (xiv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the fifth color label or dye when the fourteenth target nucleic acid is detected in the reaction vessel; (xv) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the second color label or dye and the sixth color label or dye when the fifteenth target nucleic acid is detected in the reaction vessel; (xvi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fourth color label or dye when the sixteenth target nucleic acid is detected in the reaction vessel; (xvii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the fifth color label or dye when the seventeenth target nucleic acid is detected in the reaction vessel; (xviii) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the third color label or dye and the sixth color label or dye when the eighteenth target nucleic acid is detected in the reaction vessel; (xix) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye when the nineteenth target nucleic acid is detected in the reaction vessel; (xx) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye when the twentieth target nucleic acid is detected in the reaction vessel; and (xxi) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye when the thirtieth target nucleic acid is detected in the reaction vessel.

10. The method of claim 1, wherein step (a) comprises (a) labeling at least seven different nucleic acid probes with at least three different colored labels or dyes to create a set of color codes specific for at least seven different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a 114Docket No. ABBTT-43596.601 first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a third color label or dye; (iv) a fourth nucleic acid probe specific for a fourth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (v) a fifth nucleic acid probe specific for a fifth target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (vi) a sixth nucleic acid probe specific for a sixth target nucleic acid is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; and (vii) a seventh nucleic acid probe specific for a seventh target nucleic acid is labeled with a combination of labels or dyes comprising the first color label or dye, the second color label or dye, and the third color label or dye.

11. The method of any one of claims 1-10, wherein the digital multiplexed nucleic acid amplification assay comprises PCR or isothermal amplification.

12. A method for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, comprising: (a) labeling at least three different detection agents with at least two different color labels or dyes to create a set of color codes specific for at least three different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye, (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; and (iii) a third detection agent specific for a third target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; (b) performing a digital immunoassay in parallel for each of the at least three different target antigens to detect, on average, a single one of the at least three different target antigens of interest in each immunoassay, wherein: 115Docket No. ABBTT-43596.601 (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; and (iii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the third detection agent detects the third target antigen.

13. The method of claim 12, wherein step (a) comprises labeling at least six different detection agents with at least three different colored labels or dyes to create a set of color codes specific for at least six different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of dyes comprising the first and second color label or dyes; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of dyes comprising the first color and third color label or dyes; and (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of dyes comprising the second color label or dye and the third color label or dye.

14. The method of claim 13, wherein step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least six different target antigens to detect, on average, a single one of the at least six different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; 116Docket No. ABBTT-43596.601 (iv) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the fifth detection agent detects the fifth target antigen; and (vi) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the sixth detection agent detects the sixth target antigen.

15. The method of claim 12, wherein step (a) comprises labeling at least 10 different detection agents with at least four different colored labels or dyes to create a set of color codes specific for at least 10 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the first and third color label or dyes; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first and fourth color label or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the second and third color label or dyes; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the second and fourth color label or dyes; and (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the third and fourth color label or dyes. 117Docket No. ABBTT-43596.601 16. The method of claim 15, wherein step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least 10 different target antigens to detect, on average, a single one of the at least 10 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the ninth detection agent detects the ninth target antigen; and (x) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the tenth detection agent detects the tenth target antigen.

17. The method of claim 12, wherein step (a) comprises labeling at least 15 different detection agents with at least five different colored labels or dyes to create a set of color codes specific for at least 15 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; 118Docket No. ABBTT-43596.601 (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a fifth color label or dye; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of dyes comprising the first and second color label or dyes; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of dyes comprising the first and third color label or dyes; (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the first and fourth color label or dyes; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the first and fifth color label or dyes; (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the second color and third color label or dyes; (xi) an eleventh detection agent specific for an eleventh target antigen is labeled with a combination of labels or dyes comprising the second and fourth color label or dyes; (xii) a twelfth detection agent specific for a twelfth target antigen is labeled with a combination of labels or dyes comprising the second and the fifth color label or dyes; (xiii) a thirteenth detection agent specific for a thirteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xiv) a fourteenth detection agent specific for a fourteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; and (xv) a fifteenth detection agent specific for a fifteenth target antigen is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye. 119Docket No. ABBTT-43596.601 18. The method of claim 17, wherein step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least 15 different target antigens to detect, on average, a single one of the at least 15 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the fifth color label or dye when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the seventh detection agent detects the seventh target antigen; (viii) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the first and fifth color labels or dyes when the ninth detection agent detects the ninth target antigen; (x) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the tenth detection agent detects the tenth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the eleventh detection agent detects the eleventh target antigen; 120Docket No. ABBTT-43596.601 (xii) the digital immunoassay emits a light signal corresponding to the combination of the second and fifth color labels or dyes when the twelfth detection agent detects the twelfth target antigen; (xiii) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the thirteenth detection agent detects the thirteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the third and fifth color labels or dyes when the fourteenth detection agent detects the fourteenth target antigen; and (xv) the digital immunoassay emits a light signal corresponding to the combination of the fourth and fifth color labels or dyes when the fifteenth detection agent detects the fifteenth target antigen.

19. The method of claim 12, wherein step (a) comprises labeling at least 21 different detection agents with at least six different colored labels or dyes to create a set of color codes specific for at least 21 different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a fourth color label or dye; (v) a fifth detection agent specific for a fifth target antigen is labeled with a fifth color label or dye; (vi) a sixth detection agent specific for a fifth target antigen is labeled with a sixth color label or dye; (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the second color label or dye; 121Docket No. ABBTT-43596.601 (viii) an eighth detection agent specific for an eighth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the third color label or dye; (ix) a ninth detection agent specific for a ninth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the fourth color label or dye; (x) a tenth detection agent specific for a tenth target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the fifth color label or dye; (xi) an eleventh detection agent specific for an eleventh target antigen is labeled with a combination of labels or dyes comprising the first color label or dye and the sixth color label or dye; (xii) a twelfth detection agent specific for a twelfth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the third color label or dye; (xiii) a thirteenth detection agent specific for a thirteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the fourth color label or dye; (xiv) a fourteenth detection agent specific for a fourteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the fifth color label or dye; (xv) a fifteenth detection agent specific for a fifteenth target antigen is labeled with a combination of labels or dyes comprising the second color label or dye and the sixth color label or dye; (xvi) a sixteenth detection agent specific for a sixteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fourth color label or dye; (xvii) a seventeenth detection agent specific for a seventeenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the fifth color label or dye; 122Docket No. ABBTT-43596.601 (xviii) an eighteenth detection agent specific for an eighteenth target antigen is labeled with a combination of labels or dyes comprising the third color label or dye and the sixth color label or dye; (xix) a nineteenth detection agent specific for a nineteenth target antigen is labeled with a combination of labels or dyes comprising the fourth color label or dye and the fifth color label or dye; (xx) a twentieth detection agent specific for a twentieth target antigen is labeled with a combination of labels or dyes comprising the fourth color label or dye and the sixth color label or dye; and (xxi) a twenty-first detection agent specific for a twenty-first target antigen is labeled with a combination of labels or dyes comprising the fifth color label or dye and the sixth color label or dye.

20. The method of claim 19, wherein step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least 21 different target antigens to detect, on average, a single one of the at least 21 different target antigens of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the fourth color label or dye when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the fifth color label or dye when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the sixth color label or dye when the sixth detection agent detects the sixth target antigen; (vii) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the seventh detection agent detects the seventh target antigen; 123Docket No. ABBTT-43596.601 (viii) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the eighth detection agent detects the eighth target antigen; (ix) the digital immunoassay emits a light signal corresponding to the combination of the first and fourth color labels or dyes when the ninth detection agent detects the ninth target antigen; (x) the digital immunoassay emits a light signal corresponding to the combination of the first and fifth color labels or dyes when the tenth detection agent detects the tenth target antigen; (xi) the digital immunoassay emits a light signal corresponding to the combination of the first and sixth color labels or dyes when the eleventh detection agent detects the eleventh target antigen; (xii) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the twelfth detection agent detects the twelfth target antigen; (xiii) the digital immunoassay emits a light signal corresponding to the combination of the second and fourth color labels or dyes when the thirteenth detection agent detects the thirteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the second and fifth color labels or dyes when the fourteenth detection agent detects the fourteenth target antigen; (xv) the digital immunoassay emits a light signal corresponding to the combination of the second and sixth color labels or dyes when the fifteenth detection agent detects the fifteenth target antigen; (xvi) the digital immunoassay emits a light signal corresponding to the combination of the third and fourth color labels or dyes when the sixteenth detection agent detects the sixteenth target antigen; (xvii) the digital immunoassay emits a light signal corresponding to the combination of the third and fifth color labels or dyes when the seventeenth detection agent detects the seventeenth target antigen; 124Docket No. ABBTT-43596.601 (xviii) the digital immunoassay emits a light signal corresponding to the combination of the third and sixth color labels or dyes when the eighteenth detection agent detects the eighteenth target antigen; (xix) the digital immunoassay emits a light signal corresponding to the combination of the fourth and fifth color labels or dyes when the nineteenth detection agent detects the nineteenth target antigen; (xx) the digital immunoassay emits a light signal corresponding to the combination of the fourth and sixth color labels or dyes when the twentieth detection agent detects the twentieth target antigen; and (xxi) the digital immunoassay emits a light signal corresponding to the combination of the fifth and sixth color labels or dyes when the twenty-first detection agent detects the twenty-first target antigen.

21. The method of claim 12, wherein step (a) comprises: (a) labeling at least seven different detection agents with at least three different colored labels or dyes to create a set of color codes specific for at least seven different target antigens of interest, wherein: (i) a first detection agent specific for a first target antigen is labeled with a first color label or dye; (ii) a second detection agent specific for a second target antigen is labeled with a second color label or dye; (iii) a third detection agent specific for a third target antigen is labeled with a third color label or dye; (iv) a fourth detection agent specific for a fourth target antigen is labeled with a combination of labels or dyes comprising the first and second color label or dyes; (v) a fifth detection agent specific for a fifth target antigen is labeled with a combination of labels or dyes comprising the first color and third color label or dyes; (vi) a sixth detection agent specific for a sixth target antigen is labeled with a combination of labels or dyes comprising the second and third color label or dyes; and (vii) a seventh detection agent specific for a seventh target antigen is labeled with a combination of labels or dyes comprising the first, second, and the third color label or dyes.

22. The method of claim 21, wherein step (b) comprises: (b) performing a digital immunoassay in parallel for each of the at least seven different target antigens to detect, on average, a single one of the at least seven different target antigens of interest in each immunoassay, wherein: 125Docket No. ABBTT-43596.601 (i) the digital immunoassay emits a light signal corresponding to the first color label or dye when the first detection agent detects the first target antigen; (ii) the digital immunoassay emits a light signal corresponding to the second color label or dye when the second detection agent detects the second target antigen; (iii) the digital immunoassay emits a light signal corresponding to the third color label or dye when the third detection agent detects the third target antigen; (iv) the digital immunoassay emits a light signal corresponding to the combination of the first and second color labels or dyes when the fourth detection agent detects the fourth target antigen; (v) the digital immunoassay emits a light signal corresponding to the combination of the first and third color labels or dyes when the fifth detection agent detects the fifth target antigen; (vi) the digital immunoassay emits a light signal corresponding to the combination of the second and third color labels or dyes when the sixth detection agent detects the sixth target antigen; and (vii) the digital immunoassay emits a light signal corresponding to the combination of the first, second, and third color labels or dyes when the seventh detection agent detects the seventh target antigen.

23. The method of any one of claims 1-22, wherein the digital multi-parallel immunoassay comprises a sandwich immunoassay.

24. The method of any one of claims 1-23, wherein each digital immunoassay is performed in a separate reaction vessel.

25. The method of claim 24, wherein the reaction vessel comprises a capture agent bound to a solid support in the reaction vessel.

26. The method of claim 24, wherein the reaction vessel comprises a capture agent bound to the reaction vessel.

27. A method for performing a digital multiplexed nucleic acid amplification assay to detect at least a first target nucleic acid in a sample, comprising: (a) labeling at least a first nucleic acid probe with at least two different colored dyes to create a set of color codes specific for at least a first target nucleic acid of interest, wherein: (i) the first nucleic acid probe specific for the first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a 126Docket No. ABBTT-43596.601 second color label or dye; (b) aliquoting the sample into at least one reaction vessel, wherein on average, the at least one reaction vessel contains a single molecule of the target nucleic acid and the first nucleic acid probe; (c) amplifying in the at least one reaction vessel, on average, the single molecule of the target nucleic acid; and (d) detecting in the at least one reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (i) the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when the first target nucleic acid is detected in the reaction vessel.

28. A method for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, comprising: (a) labeling at least a first detection agent with at least two different color labels or dyes to create a set of color codes specific for at least a first target antigens of interest, wherein: (i) the first detection agent specific for the first target antigen is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye, (b) performing a digital immunoassay in parallel for at least the first target antigen to detect, on average, a single one of at least the first target antigen of interest in each immunoassay, wherein: (i) the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen.

29. A kit for performing a digital multiplex nucleic acid amplification assay to detect a plurality of targets in a sample, comprising: (a) at least three different nucleic acid probes labeled with at least two different colored labels or dyes to create a set of color codes specific for at least three different target nucleic acids of interest, wherein: (i) a first nucleic acid probe specific for a first target nucleic acid is labeled with a first color label or dye; (ii) a second nucleic acid probe specific for a second target nucleic acid is labeled with a second color label or dye; and (iii) a third nucleic acid probe specific for a third target nucleic acid is labeled with a combination of dyes comprising the first color label or dye and the second color label or dye; 127Docket No. ABBTT-43596.601 (b) instructions for performing a digital multiplexed nucleic acid amplification assay, wherein the instructions instruct a user of the kit to: (i) aliquot the sample into a plurality of reaction vessels, wherein on average, each occupied reaction vessel contains a single molecule of target nucleic acid and the at least three different nucleic acid probes; (ii) amplify in each reaction vessel, on average, the single molecule of the target nucleic acid; and (iii) detect in each reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein: (1) the reaction vessel emits a light signal corresponding to the first color label or dye when the first target nucleic acid is detected in the reaction vessel; (2) the reaction vessel emits a light signal corresponding to the second color label or dye when the second target nucleic acid is detected in the reaction vessel; and (3) the reaction vessel emits a light signal corresponding to the combination of the first and second color labels or dyes when the third target nucleic acid is detected in the reaction vessel.

30. A kit for performing a digital multiplexed nucleic acid amplification assay to detect at least a first target nucleic acid in a sample, comprising: (a) at least a first nucleic acid probe labeled with at least two different colored labels or dyes to create a set of color codes specific for at least a first target nucleic acid of interest, wherein the first nucleic acid probe specific for the first target nucleic acid is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye; (b) instructions for performing a digital multiplexed nucleic acid amplification assay, wherein the instructions instruct a user of the kit to: (i) aliquot the sample into at least one reaction vessel, wherein on average, the at least one reaction vessel contains a single molecule of the target nucleic acid and the first nucleic acid probe; (ii) amplify in the at least one reaction vessel, on average, the single molecule of the target nucleic acid; and (iii) detect in the at least one reaction vessel, on average, the single molecule of the target nucleic acid based on the set of color codes, wherein the reaction vessel emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when the first target nucleic acid is detected in the reaction vessel. 128Docket No. ABBTT-43596.601 31. A kit for performing a digital multi-parallel immunoassay to detect a plurality of target antigens in a sample, comprising: (a) at least a first detection agent labeled with at least two different color labels or dyes to create a set of color codes specific for at least a first target antigens of interest, wherein (i) the first detection agent specific for the first target antigen is labeled with a combination of labels or dyes comprising at least a first color label or dye and at least a second color label or dye, (b) instructions for performing a digital multiplexed nucleic acid amplification assay, wherein the instructions instruct a user of the kit to: (i) perform a digital immunoassay in parallel for at least the first target antigen to detect, on average, a single one of at least the first target antigen of interest in each immunoassay, wherein the digital immunoassay emits a light signal corresponding to the combination of labels or dyes comprising at least the first and second color labels or dyes when at least the first detection agent detects at least the first target antigen.

32. The kit of any of claims 29-31, wherein the kit further comprises one or more reaction vessels.

33. The kit of any of claims 29-32, wherein the digital multiplexed nucleic acid amplification assay comprises PCR or isothermal amplification. 129

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