Sample processing and detection device having liquid transfer structure

Abstract

The present application relates to a sample processing and detection device having a liquid transfer structure. The sample processing and detection device comprises: an upper cover (10), wherein a central hole (11) is provided in the center of the upper cover (10); a base plate (30), wherein the base plate (30) and the upper cover (10) jointly form a hollow accommodating space, the base plate (30) is provided with a plurality of liquid accommodating spaces, a central shaft accommodating hole (38) is provided in the center of the base plate (30), and a central shaft (50) is detachably connected in the central shaft accommodating hole (38); and a liquid transfer assembly (20), wherein an upper portion of the liquid transfer assembly (20) passes through the central hole (11) and is connected to a power device, and is driven by the power device to move in a vertical direction and / or rotate around an axis by means of taking the central shaft (50) as a center; and a lower portion of the liquid transfer assembly (20) is connected to a liquid suction head (23), and the liquid suction head (23) is driven by the power device to transfer liquid between different liquid accommodating spaces.

Description

Sample processing and detection device provided with liquid transfer structure TECHNICAL FIELD

[0001] The present application relates to the field of biotechnology devices, in particular to a sample processing and detection device provided with a liquid transfer structure. BACKGROUND

[0002] Currently, the main diagnostic detection technology platforms are microorganism detection, immunological detection and molecular detection. In medical testing, the main applications are pathogenic microorganism infection detection, tumor and genetic disease diagnosis, immune system disease diagnosis, and prenatal screening.

[0003] Currently, the mainstream technology for molecular detection is fluorescent quantitative PCR technology. Due to the exponential amplification of the template by PCR technology, the aerosol pollution caused by open consumables and the requirement for separate liquid preparation and extraction in the laboratory before detection also become important factors limiting the further application of fluorescent quantitative PCR technology. In addition, traditional products have many experimental steps, complex operation, PCR aerosol pollution, low automation, and manual operation can easily lead to unstable results and difficulty in implementing multiple detection.

[0004] With the development of science and technology, there is an increasing demand for molecular diagnostic technology to be accurate, convenient, sensitive, automated and integrated. However, due to the complexity of molecular detection technology, there are few fully automated and integrated instrument platforms from sample addition to result output, or many technical problems that are difficult to solve, such as complex structure, poor sensitivity and specificity, and complex operation and detection equipment. SUMMARY

[0005] The technical problem solved by the present application is that the existing technology only realizes nucleic acid detection and cannot realize the integration of sample processing and detection. The present application provides a sample processing and detection device provided with a liquid transfer structure.

[0006] To solve the above problems, the present application provides the following technical solutions:

[0007] The present application provides a sample processing and detection device provided with a liquid transfer structure, comprising:

[0008] an upper cover, the center of the upper cover is provided with a center hole;

[0009] a bottom disc, the bottom disc and the upper cover form a hollow containing space, the bottom disc is provided with a plurality of liquid containing spaces, the center of the bottom disc is provided with a center shaft containing hole, and the center shaft containing hole is detachably connected with a center shaft;

[0010] The liquid transfer assembly is connected to the power device through the central hole of the upper part and moves vertically and / or rotates around the axis under the drive of the power device; the lower part of the liquid transfer assembly is connected to the liquid suction head, which realizes the liquid transfer between different liquid containing spaces under the drive of the power device.

[0011] Further, the liquid transfer assembly comprises:

[0012] The connecting part passes through the central hole of the upper cover and moves vertically and / or rotates around the axis in the central hole under the drive of the power device;

[0013] The platform part is connected to the liquid suction head at one end, and a downward open groove is arranged at the center of the platform part and matched with the central shaft, the central shaft can be sleeved in the groove, and the outer side of the groove and the central shaft is sleeved with a spring, the upper end of the spring is connected to the platform part, and the lower end is connected to the bottom plate.

[0014] The upper end of the spring is connected to the bottom surface of the platform part, and the lower end is connected to the bottom of the bottom plate, when the platform part moves downward under the action of external force, the spring contracts, and when the external force is removed or reduced, the spring resets to drive the platform part to move upward.

[0015] Further, the power device comprises a first driving device and a second driving device, the first driving device is connected to the connecting part to drive the liquid transfer assembly to move vertically and / or rotate around the axis; the second driving device is connected to the liquid suction head through the connecting part to perform vacuum suction.

[0016] Further, the liquid suction head comprises a suction head cavity and a steel needle, the steel needle is sealingly connected to the suction head cavity, and the suction head cavity is connected to the second driving device through the connecting part. The steel needle is an injection steel needle for puncturing the sealing film and sucking the liquid.

[0017] Further, a baffle is connected in the suction head cavity. The baffle is located in the middle section of the air passage in the suction head cavity, which prevents a small amount of liquid from splashing into the air passage in the suction head part during the liquid extraction.

[0018] Further, a hollow cavity is arranged in the connecting part, a sealing gasket is arranged in the connecting part, a sealed containing space is formed between the sealing gasket and the liquid suction head, the second driving device is connected to the sealed containing space, and the liquid suction head is sucked or discharged by the input or suction of the gas.

[0019] Further, a first filter element is arranged below the sealing gasket, and the gas enters the liquid suction head through the filter element.

[0020] Further, a sealing ring is arranged between the connecting portion and the center hole.

[0021] Further, an annular boss extending outward is arranged on the upper cover, and a sample inlet is arranged on the annular boss.

[0022] Further, the sample inlet is arranged in correspondence with the liquid transfer device.

[0023] Further, a sealing plug detachably connected with the sample inlet is arranged on the sample inlet.

[0024] Further, an air vent is arranged on the annular boss.

[0025] Further, a second filter element is arranged in the air vent.

[0026] The first filter element and the second filter element are used for filtering impurities and aerosols in air, and the first filter element can also balance the internal air pressure of the reagent box.

[0027] Further, a clamping groove is arranged on the outer ring of the base plate, and a clamping buckle corresponding to the clamping groove is arranged on the upper cover, and the clamping groove and the clamping buckle are detachably and sealingly connected.

[0028] Further, a first liquid containing hole is arranged on the base plate, and the first liquid containing hole is close to the edge of the base plate; second and third containing holes are symmetrically arranged between the first containing hole and the center hole; fourth and fifth containing holes are arranged on the outer sides of the second and third containing holes; a plurality of seventh containing holes are uniformly distributed on the side of the base plate opposite to the first containing hole; and a sixth containing hole is arranged in the region between the seventh containing hole and the fourth containing hole.

[0029] Further, a first liquid containing hole 91 is arranged on the base plate, and the first liquid containing hole 91 is close to the edge of the base plate; second and third containing holes 92 and 93 are symmetrically arranged between the first containing hole 91 and the center hole 101; fourth, fifth, seventh, eighth, ninth and tenth containing holes 94, 95, 97, 98, 99 and 100 are arranged on the outer sides of the second and third containing holes 92 and 93; and a plurality of sixth containing holes 96 are uniformly distributed on the side of the base plate opposite to the first containing hole 91.

[0030] Further, the base plate is integrally formed by a transparent high molecular material; and the transparent high molecular material is optical grade polypropylene material.

[0031] Further, a limiting hole is further arranged on the base plate, and the depth of the limiting hole is greater than the depth of the seventh containing hole.

[0032] The application has the beneficial effects that: by forming liquid transfer sample processing and detection reaction in the detection box, nucleic acid and other biological macromolecules can be prepared and detected in an automatic integrated closed space, overcoming the problems of complicated extraction and detection operation, time and labor consuming, sample or reagent pollution, inaccuracy and the like; in addition, all extraction and detection steps can be carried out in the same closed space, accelerating the detection time, and the detection result is less affected by manual operation and can be more accurate; by setting multiple detection bins, multiple diseases can be detected at the same time, greatly simplifying the diagnosis and multiple detection of similar diseases in clinic. By setting different detection holes, constant temperature amplification or PCR amplification can be carried out, and the application range is wide. BRIEF DESCRIPTION OF DRAWINGS

[0033] Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The accompanying drawings are included to provide a description of the preferred embodiments and are not intended to limit the scope of the present application. Throughout the drawings, the same reference designates the same elements. In the drawings:

[0034] Fig. 1 is a structural schematic diagram of a sample processing and detection device provided with a liquid transfer structure according to the present application;

[0035] Fig. 2 is a schematic diagram of a liquid suction head structure of a sample processing and detection device provided with a liquid transfer structure according to the present application;

[0036] Fig. 3 is a schematic diagram of a bottom plate structure of a sample processing and detection device provided with a liquid transfer structure according to the present application;

[0037] Fig. 4 is a schematic diagram of a bottom plate structure of a sample processing and detection device provided with a liquid transfer structure according to the present application;

[0038] Fig. 5 is a schematic diagram of a partial structure of a liquid suction head of a sample processing and detection device provided with a liquid transfer structure according to the present application;

[0039] Fig. 6 is a schematic diagram of another bottom plate structure of a sample processing and detection device provided with a liquid transfer structure according to the present application.

[0040] Explanation of reference signs:

[0041] 10 upper cover, 11 center hole, 12 sample inlet, 13 sealing plug, 14 vent, 15 second filter core, 20 liquid transfer assembly, 21 connecting portion, 22 platform portion, 23 liquid suction head, 231 flow baffle, 232 suction head cavity, 233 steel needle, 24 groove, 25 platform portion bottom surface, 26 sealing gasket, 27 first filter core, 28 sealing cover, 30 base plate, 31 first accommodating hole, 32 second accommodating hole, 33 third accommodating hole, 34 fourth accommodating hole, 35 fifth accommodating hole, 36 sixth accommodating hole, 37 seventh accommodating hole, 38 center shaft accommodating hole, 39 limiting hole, 40 spring, 50 center shaft; 91 first accommodating hole, 92 second accommodating hole, 93 third accommodating hole, 94 fourth accommodating hole, 95 fifth accommodating hole, 96 sixth accommodating hole, 97 seventh accommodating hole, 98 eighth accommodating hole, 99 ninth accommodating hole, 100 tenth accommodating hole, 101 center hole. Best Mode for Carrying Out the Invention

[0042] It is to be understood that the terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. The terms "comprises", "comprising", "includes", "including" and "has" are inclusive and therefore specify the presence of stated features, steps, operations, elements, and / or components, but do not preclude the presence or addition of one or more other features, steps, operations, elements, components, and / or groups thereof. The method steps, processes, and operations described herein are not to be construed as necessarily requiring their performance in the particular order

[0043] Although the terms first, second, third, etc. can be used herein to describe various elements, components, regions, layers and / or sections, these elements, components, regions, layers and / or sections should not be limited by these terms. These terms can be only used to distinguish one element, component, region, layer or section from another region, layer or section. Terms such as "first", "second", and other numerical terms when used herein do not imply a sequence or order unless clearly indicated by the context. Thus, a first element, component, region, layer or section discussed below could be termed a second element, component, region, layer or section without departing from the teachings of the example embodiments.

[0044] For the convenience of description, spatial relative terms can be used in the description to describe the relationship of one element or feature relative to another element or feature as shown in the drawings, such as "inner", "outer", "inside", "outside", "below", "above", etc. Such spatial relative terms are intended to include different orientations of the device in use or operation in addition to the orientation depicted in the drawings.

[0045] The application provides a sample processing and detecting device provided with a liquid transferring structure, comprising:

[0046] An upper cover 10 is provided with a central hole 11 at the center thereof;

[0047] A bottom plate 30 is provided with a plurality of liquid containing spaces, and the bottom plate 30 is connected with a central shaft 50 at the center thereof;

[0048] A liquid transferring assembly 20 is connected with the power device through the central hole 11, and is driven by the power device to move in the vertical direction and / or rotate around the axis with the central shaft 50 as the axis; the lower part of the liquid transferring assembly 20 is connected with a liquid suction head 23, and the liquid suction head 23 is driven by the power device to realize liquid transferring between different liquid containing spaces.

[0049] Further, the liquid transferring assembly 20 comprises a connecting part 21 and a platform part 22 connected in sequence, the connecting part 21 passes through the central hole 11 of the upper cover 10 and is driven by the power device to move in the vertical direction and / or rotate around the axis in the central hole 11; one end of the platform part 22 is connected with the liquid suction head 23, and the center of the platform part 23 is provided with a downward opening groove 24 matched with the central shaft, the central shaft 50 can be sleeved in the groove 24, and the outer side of the groove 24 and the central shaft 50 is sleeved with a spring 40, the upper end of the spring 40 is connected with the platform part 22, and the lower end is connected with the bottom plate 30.

[0050] The upper end of the spring 40 is connected with the bottom surface 25 of the platform part, and the lower end is connected with the bottom of the bottom plate 30; when the platform part moves downward under the action of external force, the spring contracts, and when the external force is removed or reduced, the spring resets to drive the platform part to move upward.

[0051] The groove and the spring matched with the central shaft are arranged to sink part of the structure of the liquid transferring assembly into the bottom plate, so that the overall height of the device can be reduced, and meanwhile, no fixed connection structure is arranged between the bottom plate and the liquid transferring assembly, so that the bottom plate can be replaced.

[0052] The power device comprises a first driving device and a second driving device, the first driving device is connected with the connecting part to drive the liquid transfer assembly to move in the vertical direction and / or rotate around the axis; the second driving device is communicated with the liquid suction head through the connecting part to perform vacuum suction.

[0053] Further, the liquid suction head 23 comprises a suction head cavity 232 and a steel needle 233, the steel needle 233 is sealingly connected with the suction head cavity 232, and the suction head cavity 232 is communicated with the second driving device through the connecting part. The steel needle 233 is an injection molded steel needle, which is used to pierce the sealing film and suck the liquid.

[0054] Further, the suction head cavity 232 is connected with a baffle 231. The baffle 231 is located in the middle section of the air duct in the suction head cavity, which is used to prevent a small amount of liquid from splashing into the air duct inside the suction head part when the liquid is extracted.

[0055] Further, the connecting part is internally provided with a hollow cavity, and the connecting part is internally provided with a sealing gasket, which forms a sealed containing space between the liquid suction head and the second driving device. The second driving device is communicated with the sealed containing space, and the liquid suction head is sucked or discharged by inputting or sucking the gas.

[0056] Further, a first filter element is arranged below the sealing gasket, and the gas enters the liquid suction head through the filter element.

[0057] Further, a sealing ring is arranged between the connecting part and the center hole.

[0058] Further, an annular boss extending outward is arranged on the upper cover, and a sample inlet is arranged on the annular boss.

[0059] Further, the sample inlet is arranged corresponding to the liquid transfer device.

[0060] Further, a sealing plug detachably connected with the sample inlet is arranged on the sample inlet.

[0061] Further, an air vent is arranged on the annular boss.

[0062] Further, a second filter element is arranged in the air vent.

[0063] The first filter element and the second filter element are used to filter impurities and aerosols in the air, and the first filter element can also balance the internal air pressure of the reagent box.

[0064] Further, the outer ring of the bottom disc 30 is provided with a clamping groove, and the upper cover 10 is provided with a buckle corresponding to the clamping groove, and the clamping groove and the buckle are detachably sealingly connected.

[0065] Further, the platform part is also provided with a sealing cover 28, which is used to seal the reaction hole on the bottom plate when the spring is compressed, so as to reduce the evaporation of the liquid in the reaction hole due to heating.

[0066] Further, as one of the schemes of the present application, the bottom plate 30 is provided with a first liquid containing hole 31, which is close to the edge of the bottom plate 30; the first containing hole 31 is symmetrically provided with a second containing hole 32 and a third containing hole 33 with the central hole 98; the outer side of the second containing hole 32 and the third containing hole 33 is provided with a fourth containing hole 34 and a fifth containing hole 35; the side of the bottom plate 30 opposite to the first containing hole 31 is provided with a plurality of uniformly distributed seventh containing holes 37; the area between the seventh containing hole 37 and the fourth containing hole 34 is provided with a sixth containing hole 36.

[0067] Further, the bottom plate 30 is also provided with a limiting hole 39, the depth of which is greater than that of the seventh containing hole 37.

[0068] Further, as another scheme of the present application, the bottom plate is provided with a first liquid containing hole 91, which is close to the edge of the bottom plate; the first containing hole 91 and the central hole 101 are symmetrically provided with a second containing hole 92 and a third containing hole 93; the outer side of the second containing hole 92 and the third containing hole 93 is provided with a fourth containing hole 94, a fifth containing hole 95, a seventh containing hole 97, an eighth containing hole 98, a ninth containing hole 99, and a tenth containing hole 100; the side of the bottom plate opposite to the first containing hole 91 is provided with a plurality of uniformly distributed sixth containing holes 96.

[0069] Further, the bottom plate 30 is integrally formed by transparent high molecular material; the transparent high molecular material is optical grade polypropylene material.

[0070] The liquid suction head is a lower funnel-shaped hollow shell, and the bottom of the liquid suction head is provided with a through hole.

[0071] The horizontal distance between the center of the bottom plate and the through hole of the liquid suction head is L1, the minimum horizontal distance between each containing hole on the bottom plate and the center of the bottom plate is Lnmin, and the maximum horizontal distance between each containing hole on the bottom plate and the center of the bottom plate is Lnmax, Lnmin < L1 < Lnmax, wherein n = 1, 2, 3, 4, 5, 6, 7.

[0072] The bottom plate 9 is integrally formed by transparent high molecular material. The transparent high molecular material can be optical grade polypropylene material. The bottom plate 13 is injection molded by optical grade polypropylene material, which has high transparency and stable chemical properties, and is mainly used for storing and reacting various reagent solutions, and can also be used for fluorescence detection. Various shapes of liquid storage cavities and reaction cavities are designed on the bottom plate 13. The liquid storage cavity is mainly used for storing reagent solutions and storing waste liquid during the reaction process, and the reaction cavity is mainly used for amplification and reaction of various reagents. The liquid storage cavity generally has a larger volume, and the outer shape of the reaction cavity is conical, similar to a PCR tube, which is beneficial to closely fit with the heating block to improve the heating efficiency.

[0073] The outer wall of each storage area of the bottom plate is designed to be thick to prevent liquid from penetrating or evaporating, and the outer wall of the reaction area is designed to be thin to improve the heat conduction efficiency during heating. The bottom plate is integrally injection molded by high transparent high molecular material, which has good optical transmission, so the device can also be used for excitation and reception of reaction fluorescence.

[0074] The second receiving hole, the third receiving hole, the fourth receiving hole and the fifth receiving hole can be a liquid storage area or a waste liquid area. The mouth of each receiving hole is designed with a sealing ring structure. If the area is set as a storage area, the sealing film can be sealed.

[0075] The first receiving hole, the sixth receiving hole and the seventh receiving hole are liquid storage areas or reaction areas. It should be noted that the above liquid receiving holes do not strictly define the storage area and the reaction area, and any area can be used as a storage area or a reaction area.

[0076] The reaction area can be matched with heating equipment.

[0077] The center hole 38 is mainly used for fixing the lower end of the liquid suction head, which is beneficial to the sinking of the liquid suction head without deviation when sucking liquid, and can also reduce the overall height of the device. The purpose of the limiting hole 33 is to raise the seventh receiving hole 37 (10-hole), considering that the seventh receiving hole 37 is mainly used for fluorescence detection, and therefore needs to maintain a high surface finish. By lengthening the limiting hole 39 to raise the seventh receiving hole 37, it can avoid the friction between the seventh receiving hole 37 and the desktop, thereby generating scratches.

[0078] The application range of the above-mentioned sample processing and detection device with liquid transfer structure includes but is not limited to: integrated design suitable for microbiological, immunological and molecular detection devices.

[0079] Taking the simultaneous detection of multiple diseases of the respiratory tract as an example, the above-mentioned device is used, and the specific method is as follows:

[0080] 1) Liquid packaging storage: the first containing hole stores magnetic bead solution; the second containing hole stores lysis solution; the third containing hole stores washing solution; the fourth containing hole stores reconstitution buffer; the fifth containing hole stores freeze-dried reverse transcriptase; the seventh containing hole (10 holes) respectively stores freeze-dried PCR enzyme for various respiratory pathogenic microorganisms.

[0081] 2) Reaction process:

[0082] 21) Add the sample containing bacteria to the No. 1 hole;

[0083] 22) The suction head transfers a certain amount of lysis solution from the No. 2 hole to the No. 1 hole, and the No. 1 hole receives a period of heating, so that the bacteria are lysed and RNA is released;

[0084] 23) During the heating of the No. 1 hole, the suction head transfers the reconstitution buffer in the No. 4 hole to the No. 5 hole and the No. 7 hole, so that the freeze-dried enzyme inside is dissolved;

[0085] 24) After the heating of the No. 1 hole is completed, the suction head transfers the waste liquid in the No. 1 hole to the No. 2 hole;

[0086] 25) The suction head transfers the washing solution in the No. 3 hole to the No. 1 hole, and the suction head repeatedly blows and sucks in the No. 1 hole to complete thorough washing;

[0087] 26) The suction head transfers the waste liquid in the No. 1 hole to the No. 3 hole;

[0088] 27) The suction head transfers the reverse transcriptase solution in the No. 5 hole to the No. 1 hole, and the No. 1 hole receives a period of heating, so that the RNA is reverse transcribed into DNA;

[0089] 28) The DNA solution in the No. 1 hole is transferred to the No. 7 hole and mixed with the PCR enzyme therein, and the No. 7 hole receives a cycle of heating at 50-95℃;

[0090] 29) During the cycle heating of the No. 7 hole, the excitation light of the matching instrument is emitted into the No. 7 hole, the fluorescence of the No. 7 hole is collected, the fluorescence signals of each hole are independently analyzed, and finally the diagnostic result of the respiratory pathogenic microorganism contained in the sample can be obtained.

[0091] In the present application, by forming a liquid transfer sample processing and detection reaction in the detection box, the preparation and detection of biological macromolecules such as nucleic acids can be realized in an automatic integrated closed space, overcoming the problems of complicated extraction and detection operation, time and labor consumption, sample or reagent contamination, inaccuracy, etc. In addition, all extraction and detection steps can be carried out in the same closed space, accelerating the detection time, and the detection result is less affected by manual operation and can be more accurate. By setting multiple detection bins, multiple diseases can be detected at the same time, greatly simplifying the diagnosis and multiple detection of similar diseases in clinical practice. By setting different detection holes, constant temperature amplification or PCR amplification can be carried out, and the application range is wide.

[0092] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or make equivalent replacements for some technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

[0093] Cross-reference of Related Applications

[0094] The present application claims priority to Chinese Patent Application No. 202421887419.9, filed on August 6, 2024, the entire contents of which are hereby incorporated by reference. Industrial Applicability

[0095] In the present application, by forming a liquid transfer sample processing and detection reaction in the detection box, the preparation and detection of biological macromolecules such as nucleic acids can be realized in an automatic integrated closed space, overcoming the problems of complicated extraction and detection operation, time and labor consumption, sample or reagent contamination, inaccuracy, etc. In addition, all extraction and detection steps can be carried out in the same closed space, accelerating the detection time, and the detection result is less affected by manual operation and can be more accurate. By setting multiple detection bins, multiple diseases can be detected at the same time, greatly simplifying the diagnosis and multiple detection of similar diseases in clinical practice. By setting different detection holes, constant temperature amplification or PCR amplification can be carried out, and the application range is wide.

Claims

1. A sample processing and detection device provided with a liquid transfer structure, characterized in that, include: The upper cover has a central hole at its center; The chassis, together with the upper cover, forms a hollow receiving space. The chassis is provided with multiple liquid receiving spaces, and a central shaft receiving hole is provided at the center of the chassis. A central shaft is detachably connected to the central shaft receiving hole. A liquid transfer assembly, the upper part of which is connected to a power device through a central hole and moves vertically and / or rotates around the central axis under the drive of the power device; the lower part of the liquid transfer assembly is connected to a liquid suction head, which realizes liquid transfer between different liquid holding spaces under the drive of the power device.

2. The sample handling and detection device provided with a liquid transfer structure according to claim 1, characterized in that, The liquid transfer assembly includes: The connecting part passes through the central hole of the upper cover and moves vertically and / or rotates around the axis within the central hole under the drive of the power device; The platform section has a liquid suction head connected to one end. The center of the platform section has a downward-facing groove that matches the central shaft. The central shaft can be fitted into the groove. A spring is fitted on the outside of the groove and the central shaft. The upper end of the spring is connected to the platform section, and the lower end is connected to the chassis.

3. The sample handling and detection device provided with a liquid transfer structure according to claim 2, characterized in that, The power unit includes a first drive unit and a second drive unit. The first drive unit is connected to the connecting part and drives the liquid transfer assembly to move vertically and / or rotate around the axis. The second drive unit passes through the connecting part and is connected to the liquid suction head to perform vacuum suction.

4. The sample handling and detection device provided with a liquid transfer structure according to claim 3, characterized in that, The liquid suction tip includes a suction tip cavity and a steel needle. The steel needle is sealed to the suction tip cavity, and the suction tip cavity passes through a connecting part and is connected to the second driving device.

5. The sample handling and detection device provided with a liquid transfer structure according to claim 4, characterized in that, A baffle is connected inside the suction head cavity.

6. The sample handling and detection device provided with a liquid transfer structure according to claim 2, wherein, The connecting part has a hollow cavity inside, and a sealing gasket is provided inside the connecting part. A sealed receiving space is formed between the sealing gasket and the liquid suction head. The second driving device is connected to the sealed receiving space shown.

7. The sample handling and detection device provided with a liquid transfer structure according to claim 6, characterized in that, A first filter element is located below the sealing gasket, through which gas enters the liquid suction head.

8. The sample handling and testing device provided with a liquid transfer structure according to claim 2, characterized in that, A sealing ring is provided between the connecting part and the central hole.

9. The sample handling and testing device provided with a liquid transfer structure according to claim 1, characterized in that, The upper cover is provided with an outwardly extending annular protrusion, and the annular protrusion is provided with a sample inlet; the sample inlet is correspondingly provided with the liquid transfer device.

10. The sample handling and detection device provided with a liquid transfer structure according to claim 9, characterized in that, The inlet is provided with a sealing plug that can be detachably connected to the inlet; the annular protrusion is provided with a vent; and a second filter element is provided inside the vent.

11. A method of sample processing and detection, characterized by, The method uses a sample processing and detection device to process and detect the sample, and the detection kit is the sample processing and detection device with a liquid transfer structure as described in claim 1.

12. The method of sample processing and detection of claim 11, wherein, The liquid transfer assembly includes: The connecting part passes through the central hole of the upper cover and moves vertically and / or rotates around the axis within the central hole under the drive of the power device; The platform section has a liquid suction head connected to one end. The center of the platform section has a downward-facing groove that matches the central shaft. The central shaft can be fitted into the groove. A spring is fitted on the outside of the groove and the central shaft. The upper end of the spring is connected to the platform section, and the lower end is connected to the chassis.

13. The method of sample processing and detection of claim 12, wherein, The power device comprises a first driving device and a second driving device, the first driving device is connected with the connecting part to drive the liquid transfer assembly to move in the vertical direction and / or rotate around the axis; the second driving device is communicated with the liquid suction head through the connecting part to perform vacuum suction.

14. The method of sample processing and detection of claim 13, wherein, The liquid suction head comprises a suction head cavity and a steel needle, the steel needle is sealingly connected with the suction head cavity, and the suction head cavity is communicated with the second driving device through the connecting part.

15. The method of sample processing and detection of claim 14, wherein, A flow baffle is connected in the suction head cavity.

16. The method of sample processing and detection of claim 12, wherein, The connecting part is internally provided with a hollow cavity, the connecting part is internally provided with a sealing gasket, the sealing gasket and the liquid suction head form a sealed containing space, and the second driving device is communicated with the sealed containing space.

17. The method of sample processing and detection of claim 16, wherein, A first filter element is arranged below the sealing gasket, and gas passes through the filter element to enter the liquid suction head.

18. The method of sample processing and detection of claim 12, wherein, A sealing ring is arranged between the connecting part and the center hole.

19. The method of sample processing and detection of claim 11, wherein, An annular boss extending outward is arranged on the upper cover, an annular boss extending outward is arranged on the upper cover, and a sample inlet is arranged on the annular boss; the sample inlet is arranged corresponding to the liquid transfer device.

20. The method of sample processing and detection of claim 19, wherein, A sealing plug detachably connected with the sample inlet is arranged on the sample inlet; an air vent is arranged on the annular boss; and a second filter element is arranged in the air vent.

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