Method for biomanufacturing organosilicon hydrogel by means of multi-enzyme cascade and use thereof

Abstract

Provided are a method for biomanufacturing an organosilicon hydrogel by means of multi-enzyme cascade and the use thereof. The method comprises the following steps: S1, using a biogas slurry from cassava-based fuel ethanol production as a raw material, and mixing the biogas slurry with Bacillus aryabhattai; S2, purifying the biogas slurry via the production of amidase and unsaturated lipase by means of Bacillus aryabhattai metabolism; S3, converting inorganic silicon in the biogas slurry into organosilicon via parallel and tandem reactions under the cascade action of multiple enzymes, which involves the production of unsaturated lipase, oxygenase and carbonic anhydrase by means of Bacillus aryabhattai metabolism; S4, cyclizing straight-chain organosilicon via parallel and tandem reactions under the cascade action of multiple enzymes, which involves the production of amidase, unsaturated lipase, oxygenase and carbonic anhydrase by means of Bacillus aryabhattai metabolism; and S5, performing freeze-drying to obtain the organosilicon hydrogel. Compared with the prior art, the present invention provides a hydrogel with antibacterial, antistatic and corrosion-resistant properties and good biocompatibility. By means of using metabolic enzymes from Bacillus, the process of converting silicate into organosilicon, which originally requires high temperature, is realized by means of biotransformation at ambient temperature. The formed hydrogel can be used for corrosion prevention and antistatic protection of devices such as boilers, and exhibits reversibility; and can also be used for antisepsis and bacteriostasis in cosmetics, and for sand fixation, water retention and promotion of plant growth in agriculture and forestry. Moreover, the hydrogel is recyclable and reusable.

Description

Method for multi-enzyme cascade bio-manufacturing of silicone hydrogel and application thereof TECHNICAL FIELD

[0001] The present application relates to the technical field of hydrogels, in particular to a method for multi-enzyme cascade bio-manufacturing of silicone hydrogel and application thereof. BACKGROUND

[0002] As a unique "soft" material, hydrogels have high swelling, mechanical properties, optical transparency, biodegradability, biocompatibility and other advantages, and are widely used in industrial, agricultural, biomedical and physiological health fields. In addition to calcium and magnesium ion scaling in boilers, there is also sulfate corrosion, and gas corrosion under the action of basic sulfate in the attached layer, that is, the molten sulfate on the heating surface absorbs SO3 in the flue gas, and under the action of Fe2O3 and Al2O3 in the flue gas, a composite sulfate (Na, K) (Fe, Al) (SO4)3 is generated, and the corrosion of the boiler is also affected by the corrosion of the alkali metal pyrosulfate 2K3Fe(SO4)3. Patent 201610303155.1 relates to a nano-silicon dioxide composite hydrogel that can be used for 3D printing and wound repair and has certain corrosion resistance. Publication No. CN202211151972.1 reduces phenylethanone to phenylethanol under the action of aldehyde ketone reductase, and is completed by one-pot method. Application (Patent) No. 202210109988 discloses a method for preparing polyester polyols based on a cascade biosynthesis system controlled by enzyme chemistry, which uses lactone as a ring-opening polymerization monomer and norbornene-based initiator as a ring-opening polymerization initiator and ring-opening metathesis polymer. Coupling application of biocatalytic polymerization and metal catalytic polymerization in a microflow control reaction platform, by adjusting the conditions of the two-step reaction, the synthesis steps are effectively simplified, and it has industrial application value (similar reference: Xu Jian and Yu Huilei. Construction of multi-enzyme cascade reaction and its application in synthesis of bifunctional functional chemicals. 2023. 39 (6): 2158-2189).

[0003] There are many types of hydrogels, and injectable hydrogels are formed in situ in the form of "sol-gel" transformation, including temperature-sensitive crosslinking, chemical addition crosslinking, free radical crosslinking, ionic crosslinking, photo-crosslinking and enzyme crosslinking. Enzyme crosslinking hydrogel uses natural enzyme as catalyst, does not introduce organic solvent, and can avoid the inactivation of bioactive molecules. The reaction rate of enzyme crosslinking hydrogel is easy to control, which can be effectively adjusted by adjusting the catalytic activity of enzyme, and the reaction is mild, which has the characteristics of green chemistry. The hydrogel prepared by physical crosslinking method has environmental response performance due to the existence of internal reversible bond, which can adjust the release rate of corrosion inhibitor in the loaded hydrogel and perform targeted release, and is applied in the field of intelligent corrosion inhibitor, which has good development prospect.

[0004] Self-assembly refers to a technology that basic structural units (molecules, nanomaterials, microns or larger scale substances) spontaneously form ordered structures. The process of self-assembly is not a simple superposition of weak forces between a large number of atoms, ions and molecules, but a simultaneous spontaneous correlation and collection of several individuals to form a close, stable and orderly whole. When the complex synergy of the whole is used in self-assembly method, it is simple and easy to operate without special device, usually uses water as solvent, can realize optical, electrical, magnetic and other functions, and even simulates biological synthesis and is highly valued. The water gel can utilize dissolved cellulose and silicon elements under alkaline conditions, and can be characterized by liquid nuclear magnetic resonance, infrared spectrum (FT-IR), scanning electron microscope (SEM), fluorescence photovoltaic instrument (FL) and mechanical property test, respectively. Although the water gel has many superior properties, its mechanical properties are insufficient, which limits its development in many fields. Under the premise of not damaging the performance of the water gel, the self-assembly with the biological membrane (biofilm) can improve the mechanical properties of the water gel, which can be used for automatic descaling of boilers and other equipment and recycling, and is an important research direction of the water gel, and there is no specific report. SUMMARY

[0005] The present application is to overcome the shortcomings of the prior art, and provides a method for producing organosilicon hydrogel by multi-enzyme cascade biological manufacturing and application.

[0006] To achieve the above-mentioned purpose, a method for producing organosilicon hydrogel by multi-enzyme cascade biological manufacturing is designed, which comprises the following steps:

[0007] S1, taking the biogas liquid for fuel ethanol of cassava as raw material, mixing the biogas liquid with Bacillus aryabhattai,

[0008] S2, the Bacillus aryabhattai metabolizes to produce amylase and unsaturated lipase, and purifies the biogas liquid;

[0009] S3, the Bacillus aryabhattai metabolizes to produce unsaturated lipase, oxygenase and carbonic anhydrase, and performs parallel and series reactions under the multi-enzyme cascade effect, so as to convert inorganic silicon in the biogas liquid into organosilicon;

[0010] S4, the amylase, unsaturated lipase, oxygenase and carbonic anhydrase produced by the Bacillus aryabhattai metabolize to perform parallel and series reactions under the multi-enzyme cascade effect, so as to circularize the linear organosilicon.

[0011] S5, freeze-drying to obtain organosilicon hydrogel.

[0012] [Corrected according to Rule 91 on 14.02.2025] In step S1, the classification name of Bacillus aryabhattai is Bacillus aryabhattai, the preservation address is China Center for Type Culture Collection, the preservation number is CCTCC NO: M20232038, the preservation time is November 1, 2023, and the preservation address is Wuhan University, School of Life Sciences, Wuhan, Hubei, China 430072.

[0013] In step S1, the biogas slurry of cassava fuel ethanol contains amino polyphenol, cassava peptide, amino acid, fulvic acid and nutrient elements including nitrogen, phosphorus, potassium, silicon, calcium and iron. Bacillus aryabhattai is inoculated in BL medium and mixed with biogas slurry.

[0014] In step S2, the biogas slurry is selectively complexed with P, Ga and Fe under the action of amidase and unsaturated lipase at 35-50℃ for 30-60min and pH 6.8-7.5 to precipitate and remove salt and purify the biogas slurry.

[0015] In step S3, methanethiolase is added for reaction, wherein the proportions of unsaturated lipase, oxygenase, methanethiolase and carbonic anhydrase are 2-5%, 6-12%, 3-9% and 10-20% respectively; the ratio of the mixture of unsaturated lipase, oxygenase, methanethiolase and carbonic anhydrase to biogas slurry is 1:2000-10000.

[0016] In step S4, the ratio of the mixture of amidase, unsaturated lipase, oxygenase and carbonic anhydrase to biogas slurry is 1:1000-5000, and the proportions of the product obtained in step S3, amidase, unsaturated lipase, oxygenase and carbonic anhydrase are 7-15%, 5-10%, 3-9% and 15-30% respectively.

[0017] In step S5, the hydrogel obtained after freeze-drying has a porosity of 85%, a specific surface area of 302-320mg / cm2, a density of 0.125-0.26g / cm3 and a pore size of 15nm-10um.

[0018] The hydrogel can be applied to boilers, steam turbines, centrifuges and other water-contacting equipment by responding to pH and controlling the operation of the module, and self-assembled with biological membranes.

[0019] The hydrogel can be applied to cosmetics by responding to pH and temperature and self-assembling with biological membranes.

[0020] The hydrogel is used for sand fixation, water conservation and plant growth promotion in agriculture and forestry.

[0021] Compared with the prior art, the hydrogel has good antibacterial, antistatic, anticorrosion and biocompatibility, the process of converting silicate into organic silicon at high temperature is biologically converted at normal temperature by using Bacillus metabolic enzyme, the formed hydrogel can be used for anticorrosion and antistatic of boilers and other equipment, has reversibility, can be used for cosmetic preservation and bacteriostasis, sand fixation and water conservation in agriculture and forestry, and plant growth promotion, and can be recycled and utilized. BRIEF DESCRIPTION OF DRAWINGS

[0022] Fig. 1 is a schematic diagram of ethyl anthranilate of the present application, which is a component extracted from the fuel ethanol biogas of cassava in step S1.

[0023] Fig. 2 is a schematic diagram of dimethyl iso-octylate (organic silicon) of the present application, which is a process of converting inorganic silicon in raw materials into organic silicon from the fuel ethanol biogas of cassava in step S2.

[0024] Fig. 3 is a schematic diagram of octamethyl tetrasiloxane (organic silicon) of the present application, which is a process of straight chain cyclization into a precursor structure of hydrogel in step S3.

[0025] Fig. 4 is a schematic diagram of octadecamethyl nonasiloxane (organic silicon) of the present application, which is a structure of organic silicon hydrogel converted through step S3. DETAILED DESCRIPTION

[0026] The present application is further described below according to the drawings. Example 1

[0027] The method for manufacturing organic silicon hydrogel by multi-enzyme cascade in the present embodiment comprises the following steps:

[0028] S1, using the biogas of fuel ethanol of cassava as raw material, mixing the biogas with Bacillus aryabhattai,

[0029] S2, the amylase and unsaturated lipase produced by the metabolism of Bacillus aryabhattai purify the biogas;

[0030] S3, the unsaturated lipase, oxygenase and carbonic anhydrase produced by the metabolism of Bacillus aryabhattai perform parallel and series reactions under the cascade action of multiple enzymes, and convert the inorganic silicon in the biogas into organic silicon, which is an anticorrosion component of the hydrogel;

[0031] S4, the amylase, unsaturated lipase, oxygenase and carbonic anhydrase produced by the metabolism of Bacillus aryabhattai perform parallel and series reactions under the cascade action of multiple enzymes, and circularize the straight chain organic silicon, thereby increasing the viscosity strength of the hydrogel;

[0032] S5, freeze-drying to obtain the organic silicon hydrogel.

[0033] The biogas slurry of the embodiment is subjected to parallel, series and progressive reactions under the action of multiple enzymes such as amidase, unsaturated lipase, oxygenase, methylthio synthase and carbonic anhydrase, can capture CO2 in the reaction tank, increase the biological activity of the multiple enzyme cascade and has the performance of cyclic use.

[0034] In step S1 of the embodiment, the classification name of the Bacillus aryabhattai is Bacillus aryabhattai, the preservation address is China Center for Type Culture Collection, the preservation number is CCTCC NO: M20232038, and the preservation time is November 1, 2023.

[0035] The raw material for producing metabolic enzymes by the Bacillus aryabhattai is the BL medium, the biogas slurry for producing ethanol by saccharifying cassava, the amino polyphenol, the cassava peptide, the amino acid and the fulvic acid, and the complex ions such as Ca and Fe are selectively removed at a ratio of 1:1:1:2 with a total content of 5%. A plurality of enzymes obtained by deep fermentation of specific Bacillus aryabhattai and having a total enzyme activity CFU / g are subjected to multiple enzyme cascade action at 40°C for 60 min. The polar groups such as -OH, -COOH and -NH2 are good hydrophilic, and the functional groups are subjected to parallel and series reactions such as addition and cyclization condensation through the structure complementation of -Si-O, so as to convert the inorganic silicon in the biogas slurry into branched and cyclized organosilica hydrogel.

[0036] In step S1, the biogas slurry for producing fuel ethanol by cassava contains amino polyphenol, cassava peptide, amino acid, fulvic acid and nutrient elements including nitrogen, phosphorus, potassium, silicon, calcium and iron. The Bacillus aryabhattai is inoculated in the BL medium and then mixed with the biogas slurry.

[0037] In step S2, the biogas slurry is subjected to selective complexation and P, Ga and Fe precipitation under the action of amidase and unsaturated lipase at 35-50°C for 30-60 min and at pH 6.8-7.5, so as to purify the biogas slurry.

[0038] In step S3, methylthio synthase is added for reaction, and the proportions of unsaturated lipase, oxygenase, methylthio synthase and carbonic anhydrase are 2-5%, 6-12%, 3-9% and 10-20% respectively. The proportion of the mixture of unsaturated lipase, oxygenase, methylthio synthase and carbonic anhydrase to the biogas slurry is 1:2000-10000.

[0039] In step S4, the proportion of the mixture of amidase, unsaturated lipase, oxygenase and carbonic anhydrase to the biogas slurry is 1:1000-5000, and the proportions of the product obtained in step S3, amidase, unsaturated lipase, oxygenase and carbonic anhydrase are 7-15%, 5-10%, 3-9% and 15-30% respectively.

[0040] The hydrogel obtained after freeze-drying in step S5 has a porosity of 85%, a specific surface area of 302-320 mg / cm2, a density of 0.125-0.26 g / cm3, and a pore size of 15 nm-10 um.

[0041] In specific use, the hydrogel can be used in devices in contact with water such as boilers, steam turbines, and centrifuges by pH response and control of the operation of the module, and applied to self-assembly with biological membranes.

[0042] In specific use, the hydrogel can be used in cosmetics by pH response and temperature response, and self-assembly with biological membranes for antiseptic and antibacterial preservation.

[0043] In specific use, the hydrogel is used for sand fixation, water conservation, and plant growth promotion in agriculture and forestry.

[0044] To verify the antiseptic performance of the silicone hydrogel obtained in this embodiment, an anti-corrosion test was performed on the silicone hydrogel: gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli) were selected for the antibacterial test of the sample, and the bacterial concentration was 106 / (CUF / ml). After adding the hydrogel to the culture, the effective inhibition rate reached 99.0-99.9%, and the E. coli was almost not observed after adding the hydrogel within 24 hours, showing excellent antibacterial activity. Example Two

[0045] This embodiment only illustrates the differences from Example One, and the same parts are not repeated.

[0046] This embodiment differs from Example One in that the Bacillus aryabhattai in step S1 of this embodiment is purchased from Shanghai Jianke Biological Technology Co., Ltd. as a freeze-dried product, stored at 4-10℃, with the number SHBCC D53239 and the Latin name Bacillus aryabhattai, preserved at the Shanghai Collection Center of Biological Technology. Example Three

[0047] This embodiment creates a reversible silicone hydrogel for antiseptic and antistatic purposes.

[0048] Take cassava sugar ethanol biogas 1000g, containing amino polyphenol, cassava peptide, amino acid, fulvic acid, with 1:1:1:2, total content 5% ratio, optional removal of Ca, Fe and other complex ions, then add by preservation number M20232036 bacillus deep fermentation obtained amidase, unsaturated lipase, oxygenase, sulfurase, carbonic anhydrase to total enzyme activity CFU / g, at 40℃, through 60min multi-enzyme cascade, in -OH, -COOH, -NH2, etc. The good hydrophilicity of the polar group, through the complementary -Si-O structure, the addition and cyclization condensation between functional groups, etc. Parallel, series reaction, the inorganic silicon in the biogas is converted into branched and cyclized organic silicon. Through further capture of CO2 in the reaction tank, progressive reaction is carried out, and three-dimensional cross-linked network is formed by covalent bond. Through temperature effect, organic silicon hydrogel with stable structure and good mechanical properties is formed, with porosity and pore size of 15nm-10um accounting for 80%, specific surface area / mg / cm2, 302-320 density / (g / cm3), 0.125-0.26 hydrogel, salt sensitivity, pH responsiveness, and reversible properties of repeated freezing and thawing at-15℃ and repeated depolymerization at 50℃, and recycling. Example four

[0049] This example is the application of organic silicon hydrogel in boiler, steam turbine and centrifuge.

[0050] The organic silicon hydrogel of example three is added to the boiler cold water system at a concentration of 0.1% at 35℃. After continuous operation for 2 weeks, when the biofilm appears on the outer wall of the boiler shell, it self-assembles with the hydrogel, enhancing the mechanical properties. By continuing to lower the temperature, the hydrogel together with the biofilm is precipitated and separated from the boiler cooling water system. After heating to 50℃, impurities can be separated and the hydrogel can be continuously used. Example five

[0051] This example is the corrosion prevention performance and test of organic silicon and hydrogel.

[0052] The organic silicon hydrogel of example three is used in the centrifuge. It can change its swelling degree with changes in environmental pH, temperature, etc. The hydrogel controls the release and responds to the small changes in the environment (such as temperature or H value). The corrosion-resistant organic silicon hydrogel is used in the field of mechanical skin, i.e. the hydrogel is coated on the metal equipment such as centrifuge. The coating has a soft and smooth surface, which can greatly reduce the degree of wear and tear. The formed biofilm can also be loaded in the hydrogel, which has the performance of reducing the corrosion degree of the centrifuge by 85% and preventing equipment fouling. By using the hydrophilic and oleophobic properties of the hydrogel, the hydrogel can selectively separate oil stains from water in the centrifuge by changing the temperature, and the efficiency can reach 99%, which is conducive to the recycling of organic silicon hydrogel.

[0053] Anti-corrosion test of silicone hydrogel: salt concentration CaCL2 0.025 / ml, gram-positive bacteria (S. aureus) and gram-negative bacteria (E. coli) were selected to test the bacteriostatic property of the sample, and the concentration of bacteria was 10 6 (CUF / ml), after adding the hydrogel, the bacteria were cultured at 37°C for 12h, and the size of the filter paper bacteriostatic ring was observed. The effective inhibition rate reached 98.0%, and the concentration of E. coli decreased by 100 times within 24 hours. Almost no E. coli was observed after adding the hydrogel, showing excellent bacteriostatic activity. Example six

[0054] This example is a test of the antistatic property of silicone hydrogel.

[0055] As in Example Three, the silicone hydrogel prepared by the interaction of ionic and covalent bonds can be prepared: (1) physical hydrogel formed by electrostatic interaction between polyelectrolyte and oppositely charged multivalent ions; (2) polyelectrolyte complex hydrogel formed by electrostatic interaction between two oppositely charged polyelectrolytes. The antistatic property test: the ionically crosslinked hydrogel prepared by the antistatic effect forms a hydrogel by proton transfer electrostatic interaction, which has a response behavior to electric field and electrolyte, and the non-covalent crosslinking network formed by physical crosslinking can be converted into a solution by heating the gel, forming a thermoreversible hydrogel. The mixed hydrogel can be stretched by 8-10 times its original length, which is the normal value. The mechanisms of electrostatic interaction, hydrogen bonding and host-guest interaction can be used for self-repair. Colorless and transparent, does not affect the color and transparency of the glue, good temperature resistance, test shows: -20°C, the test resistance value is 9 times, 320°C high temperature does not decompose. Can withstand high processing temperature. Stable chemical properties, will not react with other additives in the glue. Good compatibility, stable product properties, good fusion, long antistatic time. The addition amount is as low as 3%-5%, the surface resistance can reach 8 times, effectively controlling the production cost. Comply with environmental protection requirements, pass FDA, RoHS detection certification. Good dispersibility, the substrate adhesion strength can reach 900 J / m2. Suitable for dry state long-term storage, further improving its transportability and application simplicity. Example seven

[0056] This example is the use of silicone hydrogel in cosmetics and the use of silicone hydrogel in agriculture and forestry sand fixation, water conservation and plant growth promotion.

[0057] As in Example Three, the silicone hydrogel is self-assembled with titanium dioxide in cosmetics under photocatalysis to play a skin care role, and the concentration of bacteria is 10 6 (CUF / ml), the concentration of fungi is 10 3The water gel has the bacteriostatic activity of 1.5*10^7 CFU / ml; after adding 0.1% of the water gel in skin care products, no deterioration and no E. coli and other pathogenic bacteria are observed within one year, and the water gel has excellent bacteriostatic activity.

[0058] The water gel for sand fixation, water conservation and plant growth promotion in agriculture and forestry has the action of bacillus and metabolic amylase, unsaturated lipase, oxygenase, sulfurase and carbonic anhydrase, and the biofilm action of soil and plant seeds, and the effectiveness of the water gel for plant growth promotion is increased by 25-40%.

[0059] The present application uses biogas slurry of fuel ethanol of cassava as raw material, and under the cascade action of bacillus and metabolic enzymes, such as amylase, unsaturated lipase, oxygenase and carbonic anhydrase, the biogas slurry is selectively purified, inorganic silicon is converted into organic silicon, and then is converted into water gel through linear cyclization, and is prepared by freeze-drying. The water gel for water contact equipment, such as boiler, steam turbine and centrifuge, has enhanced mechanical properties through self-assembly with biofilm, increased reversibility, can prevent corrosion and static electricity, and can be used for cosmetic preservation and bacteriostasis, sand fixation, water conservation and plant growth promotion in agriculture and forestry.

Claims

1. A method of multi-enzyme cascade biomanufacturing of a silicone hydrogel, characterized in that: It comprises the following steps: S1, taking the biogas slurry of cassava fuel ethanol as raw material, mixing the biogas slurry with Bacillus aryabhattai, S2, Bacillus aryabhattai metabolizes to produce amidase and unsaturated lipase to purify the biogas slurry; S3, Bacillus aryabhattai metabolizes to produce unsaturated lipase, oxygenase and carbonic anhydrase, and under the action of multi-enzyme cascade, parallel and series reactions are carried out to convert inorganic silicon in the biogas slurry into organic silicon; S4, the amidase, unsaturated lipase, oxygenase and carbonic anhydrase produced by Bacillus aryabhattai metabolize to carry out parallel and series reactions under the action of multi-enzyme cascade to circularize linear organic silicon; S5, freeze-drying to obtain organic silicon hydrogel.

2. The method of multi-enzyme cascade biomanufacturing a silicone hydrogel of claim 1, wherein: In step S1, the classification name of Bacillus aryabhattai is Bacillus aryabhattai, which is preserved in China Center for Type Culture Collection, with the preservation number CCTCC NO: M20232038 and the preservation time of November 1, 2023.

3. The method of multi-enzyme cascade biomanufacturing a silicone hydrogel of claim 1, wherein: In step S1, the biogas slurry of cassava fuel ethanol contains aminopolypenol, cassava peptide, amino acid, fulvic acid and nutrient elements including nitrogen, phosphorus, potassium, silicon, calcium and iron; Bacillus aryabhattai is inoculated in BL medium and then mixed with the biogas slurry.

4. The method of multi-enzyme cascade biomanufacturing a silicone hydrogel of claim 1, wherein: In step S2, the biogas slurry is selectively complexed with P, Ga and Fe to precipitate and remove salt under the action of amidase and unsaturated lipase at 35-50℃ for 30-60 min and at pH 6.8-7.5 to purify the biogas slurry.

5. The method of multi-enzyme cascade biomanufacturing a silicone hydrogel of claim 1, wherein: In step S3, methylthio synthase is added for reaction, and the proportions of unsaturated lipase, oxygenase, methylthio synthase and carbonic anhydrase are 2-5%, 6-12%, 3-9% and 10-20% respectively; the mixture of unsaturated lipase, oxygenase, methylthio synthase and carbonic anhydrase is mixed with the biogas slurry at a ratio of 1:2000-10000.

6. The method of multi-enzyme cascade biomanufacturing a silicone hydrogel of claim 1, wherein: In step S4, the mixture of amidase, unsaturated lipase, oxygenase and carbonic anhydrase is mixed with the biogas slurry at a ratio of 1:1000-5000, and the proportions of the product obtained in step S3, amidase, unsaturated lipase, oxygenase and carbonic anhydrase are 7-15%, 5-10%, 3-9% and 15-30% respectively.

7. The method of multi-enzyme cascade biomanufacturing a silicone hydrogel of claim 1, wherein: The water gel obtained after freeze-drying in step S5 has a porosity of 85%, a specific surface area of 302-320 mg / cm 2 , a density of 0.125-0.26 g / cm 3 , and a pore size of 15 nm-10 um.

8. Use of a multi-enzyme cascade for the biosynthesis of a silicone hydrogel according to claim 1, characterized in that: The hydrogel can be applied to devices in contact with water such as boilers, steam turbines and centrifuges by pH response and control of the module operation, and self-assembled with biological membranes.

9. Use of a multi-enzyme cascade for the biosynthesis of a silicone hydrogel according to claim 1, characterized in that: The hydrogel can be applied to cosmetic preservation and inhibition of bacteria by pH response and temperature response and self-assembled with biological membranes.

10. Use of a multi-enzyme cascade for the biosynthesis of a silicone hydrogel according to claim 1, characterized in that: The hydrogel is used for sand fixation, water conservation and plant growth promotion in agriculture and forestry.

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