Pesticidal composition containing iprobenfos

Abstract

An agrochemical composition comprising (A) Iprobenfos and (B) Validamycin, (C) Tebuconazole. The invention further relates to formulations comprising the composition of the present invention and a method of controlling pests infesting plants, especially crops.

Description

[0001] PESTICIDAL COMPOSITION CONTAINING IPROBENFOS FIELD OF THE INVENTION The present disclosure relates generally to the field of agrochemical compositions. Particularly, the present disclosure pertains to a synergistic composition and formulations thereof capable of protecting crops from the harmful effects of pathogenic fungi. BACKGROUND OF THE INVENTION Fungicides are compounds, of natural or synthetic origin, which act to protect and cure plants against damage caused by agriculturally-relevant fungi. The ecological and economic demands made on modern active ingredients, for example fungicides, are increasing constantly, for example with respect to activity spectrum, toxicity, selectivity, application rate, formation of residues and favourable manufacture. There are also problems, for example, that are associated with resistances. There is thus a constant need to develop novel fungicidal compositions with different modes of action which have advantages over the known compounds and compositions at least in some areas. Hence, a premix of the best possible formulation for a combination of fungicides would help growers to combat these important fungal diseases. A typical challenge in the field of crop protection is to reduce the dose rates of an active ingredient in order to reduce or avoid unfavorable environmental or toxicological effects whilst still allowing effective control. Therefore, a combined application of an effective amount of fungicides in a uniform composition is a practical necessity. The present invention relates to a fungicidal product that contains a combination of active substances, a method for controlling undesired pathogenic microorganism using this product, its use and the plant propagation organs treated with this product, as well as the use of this combination for the preparation of the product Iprobenfos (Iprobenfos), molecular formula: C13H21O3PS, chemical name: O- diisopropyl-S-benzyl phosphorothioate sulfide .it belongs to a systemic bactericide and has good systemic bactericidal action. Absorbed by the roots of the plants and the leaf sheaths under the water surface and dispersed to all parts of the plants. The composition is mainly used for preventing and treating the neck blast of early and late rice, has obvious effects on small-pellet sclerotinia rot, small-pellet sclerotinia rot and sheath blight of rice and preventing and treating plant hoppers, and also has the function of lodging resistance. Validamycin (English common name: Validamycin) with the molecular formula of C20H35O13N is an antibiotic produced by actinomycetes, has strong systemic property, is easily absorbed by thalli cells and rapidly conducts in the thalli cells, interferes and inhibits the growth and development of the thalli cells, and pure products are white amorphous powder, are dissolved in water and dimethylformamide, are slightly dissolved in ethanol, are insoluble in organic solvents such as acetone, benzene, ethyl acetate and the like, have strong hygroscopicity, are stable in a pH3-9 aqueous solution at room temperature, are mainly used for rice sheath blight, can also be used for rice false smut, corn big and small leaf spot, crop diseases of vegetables, cotton, beans and the like, and has the advantages of high control efficiency, no chemical injury, no pollution and the like. Tebuconazole is used in a number of different popular fungicide products to control fungi, bacteria, and viruses affecting plants. Some of the common fungal and disease problems Tebuconazole is known to treat are rust fungus, sheath blight, leaf spot, and anthracnose. Tebuconazole is dimethylase inhibitor (DMI) -interferes in process of building the structure of fungal cell wall. Finally it inhibits the reproduction and further growth of fungus. Patent documents WO2018025192, CN102835407, CN101700028 and journal documents Plant Disease, 87(7), 784-788(2003) and Pestology, 38(1), 47-51(2014) also describes the composition of Azoxystrobin and Mancozeb. Further, repeated and exclusive application of individual pesticide compounds leads to development of natural or adapted resistance against the active compounds. Therefore, there is a need for pest control agents that help in preventing resistance induced by pesticides. It has been found that, as a solution to the above-mentioned problems, a combination comprising Iprobenfos, Validamycin, and Tebuconazole provides an effective composition in controlling a wide variety of undesired pathogenic microorganisms. The present invention provides a combination of Iprobenfos, Validamycin, and Tebuconazole with enhanced efficacy and spectrum as compared to the use of Iprobenfos, Validamycin, and Tebuconazole alone. OBJECT AND ADVANTAGES OF THE INVENTION: It is an object of the present invention to provide a novel and synergistic fungicidal composition demonstrating high efficacy and high selectivity. It is another object of the present invention to provide a novel and effective fungicidal composition for controlling the harmful pests and fungi in plants. It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which can be easily formulated. It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is ideal for fungicide resistance management. It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which uses lesser amounts of the actives as compared to the actives when used alone. It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is environmentally safe, possesses broad spectrum bio- efficacy, is less toxic in terms of phytotoxicity to plants and dermal and inhalational toxicity to humans. SUMMARY OF THE INVENTION: Accordingly, in one aspect, the present invention provides a fungicidal composition comprising Iprobenfos, Validamycin, and Tebuconazole. In another aspect, the present invention provides a combination of Iprobenfos, Validamycin, and Tebuconazole to control a wide variety of undesired pathogenic microorganisms. In one aspect, the present invention provides a synergistic composition of Iprobenfos, Validamycin, Tebuconazole, and agrochemically acceptable additives. In yet another aspect, the present invention provides a synergistic composition comprising Iprobenfos, Validamycin, and Tebuconazole; the composition possesses fungicidal activity. In a further aspect, the present invention provides a method for effective control of various fungi in plants. In one aspect of the present invention, the fungicidal composition of the present invention further comprises an agrochemically acceptable excipients selected from the group consisting of Emulsifiers anti-freezing agent, dispersing agents, wetting agents, antifoaming agents, inert carriers biocides, thickeners, solvents, and the like. Additional components may also be included, e.g. thickeners, stabilizers, More generally, the active materials can be combined with any solid or liquid additive, formulation aids that complies with usual formulation techniques. In another aspect of the present invention, the fungicidal composition is formulated Emulsifiable concentrate (EC), Suspension Concentrate (SC), and Suspo-emulsion (SE). In yet another embodiment of the present invention, the invention further provides a process for preparation of the said formulation, wherein the said formulation can be Emulsifiable concentrate (EC), Suspension Concentrate (SC), and Suspo-emulsion (SE). DETAILED DESCRIPTION OF THE INVENTION Discussed below are some representative embodiments of the present invention. The invention, in its broader aspects, is not limited to the specific details and representative methods. The illustrative examples are described in this section in connection with the embodiments and methods provided. The invention, according to its various aspects, is particularly pointed out and distinctly claimed in the appended claims read in view of this specification and appropriate equivalents. It is to be noted that, as used in the specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and / or” unless the content clearly dictates otherwise. The expression of various quantities in terms of “% w / w” or “%” means the percentage by weight, relative to the weight of the total composition unless otherwise specified. The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control of Fungal-pests or disease. As used herein, the terms "comprises", "comprising", "includes", "including", or any other variation thereof, are intended to cover a non-exclusive inclusion, subject to any limitation explicitly indicated. For example, a composition or a method that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, or method. As used herein, the term “composition” or "formulation" can be used interchangeably, unless stated otherwise, is meant to encompass, and is not limited to, compositions or formulations containing the combination of Iprobenfos, Validamycin, and Tebuconazole. As used herein, the term “additive(s)” or "auxiliary agent(s)" or “agrochemically acceptable carrier(s)” can be used interchangeably and refers to inert substances which are commonly used as diluent, to provide stability or to increase the activity profile of the composition or formulation with or without having agrochemical activity or direct effect on the undesired phytopathogenic fungi and / or microorganisms. As used herein, the term "agrochemically acceptable salts" are typically acid addition salts of inorganic or organic acids, preferably of hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, perchloric acid, phosphoric acid, formic acid, acetic acid, trifluoroacetic acid, oxalic acid, malonic acid, toluene sulfonic acid or benzoic acid. As used herein the term “fungicidal” refers to the ability of a composition or substance to reduce the rate of fungal growth or increase fungal mortality. As used herein, the term "effective amount" means the amount of the active substances in the compositions to achieve an observable effect on growth, including the effects of necrosis, death, retardation, prevention, and removal, destruction, or otherwise diminishing the occurrence and activity of the target organism. The effective amount can vary for the various compositions used in the present invention. An effective amount of the compositions will also vary according to the prevailing conditions such as desired pesticidal effect and duration, weather, target species, locus, mode of application, and the like. In an embodiment, the fungicidal composition wherein Iprobenfos ranging from 5% to 17.5% by weight of the fungicidal composition. In another preferred embodiment of the present invention, Iprobenfos is present in an amount of 17.50% weight of the fungicidal composition. In an embodiment, the fungicidal composition wherein Validamycin ranging from 2% to 10.0% by weight of the fungicidal composition. In another preferred embodiment of the present invention, Validamycin is present in an amount of 2.5% weight of the fungicidal composition In an embodiment, the fungicidal composition wherein Tebuconazole ranging from 5% to 15.0% by weight of the fungicidal composition. In another preferred embodiment of the present invention, Tebuconazole is present in an amount of 9 % weight of the fungicidal composition. In another embodiment of the present invention, the invention further provides the process for preparation of the said formulation wherein, the said formulation can be one or more of as suspension concentrate (SC), wettable granules (WG), wettable powder (WP), a water dispersible granule (WDG), a water dispersible tablet (WT), an ultra-low volu (ULV) liquid (UL), an ultra-low volume (ULV) suspension (SU), a water soluble powder (SP), a suspo-emulsion (SE), granule (GR), an emulsifiable granule (EG), an oil-in-water or water in oil emulsion (EW), an emulsifiable concentrate (EC), a micro-emulsion (ME), an oil dispersion (OD), a capsule suspension (CS), a dustable powder (DP) or an aerosol (AE). In preferred embodiment of the present invention, the formulation is Suspension Concentrate (SC), Suspo-emulsion (SE), oil dispersion (OD), Water dispersible granules (WDG), Granules (GR) and Wettable powders (WP). In yet another embodiment of the present invention, the agrochemically acceptable excipients of the formulation are selected from the group consisting of Emulsifiers, dispersing agents, wetting agents, antifoaming agents, thickeners, solvents, inert carrier, biocides, and anti-freezing agent . Emulsifiers is selected from the group comprising of, but not limited to ethoxylatedpropoxylated alcohols, alkylphenolethoxylates, alkoxylatedtristyrylphenols, calcium dodecylbenzenesulfonate, mixture of fatty acid polyethylene glycol ester, ethoxylated propoxylatedpolyaryl phenol, ethoxylated fatty acids, fatty alcohol ethoxylates, ethoxylated ricinoleic acid triglycerides, sorbitan trioleate, tridecyl alcohol ethoxylate, castor oil ethoxylate, alkoxylated phosphate ester or mixtures thereof.The Emulsifiers is present in an amount of from 0.1% to 20.0% by weight based on a total weight of the composition. Solvent is selected from the group comprising of, but not limited to solvents are water, oils of vegetable, cyclohexanone, Heavy aromatic solvent 2-butoxyethanol, or derivatives thereof. The Solvent is present in an amount of from 5.0 - 25.0%by weight based on a total weight of the composition. Wetting agent is selected from the group comprising of, but not limited to non-ionic proprietary surfactant blend alkylphenol ethoxylates or polyoxyethylene sorbitan esters, lignosulfonates, sodium salt of naphthalene sulfonate condensates, tristyrylphenol ethoxylates. In a preferred embodiment, the wetting cum dispersing agentin an amount of from 0.1% to 4.0% by weight based on a total weight of the composition. Antifoaming agent is selected from the group comprising of, but not limited to polydimethyl siloxane, polydimethyl siloxane emulsion or mixtures thereof; The Antifoaming agent is present in an amount of from 0.10 – 1.0% by weight based on a total weight of the composition. Anti freezing agents is selected from the group comprising of, but not limited to selected from the group comprising of ethylene glycol, propane-1,2-diol, propane-15 1,2,3-triol, urea or mixtures thereof. The Anti freezing agents is present in an amount of from 3.0 – 10.0% by weight based on a total weight of the composition. Biocide is selected from the group comprising of 1,2-benzisothiazolin-3-one, formaldehyde, dipropyl glycol solution of 1,2-benzisothiazolin-3-one or mixtures thereof. The Biocide is present in an amount of from 0.10 – 1.0% by weight based on a total weight of the composition. Dispersing agent is selected from the group comprising of, but not limited to polymeric ester dispersant, ethoxylated polyarylphenol phosphate ester, sodium salt of naphthalene sulfonate condensate / naphthalene sulphonic acid condensate, acrylic copolymer, nonionic proprietary surfactant blend, polycarboxylates, calcium dodecylbenzene sulfonate, aryl sulphonate condensate, sodium lignosulphonate, dispertox BS SPL, polystyrenatedacrylated co-polymer, modified styrene acrylic copolymer, salts of phenol sulfonic acids, Terwet 2700,butyl polyalkylene oxide block co-polymer, mixture of tristyrylphenolethoxylates and polyalkylene oxide derivative of a synthetic alcohol, Kraft lignin sulphonate, random co-polymer of alcoxylated polyethylene glycol or mixtures thereof;The Dispersing agent is present in an amount of from 2.0 – 6.0% by weight based on a total weight of the composition. Inert carriers is selected from the group comprising of, but not limited to kaolin, china clay, alumina, talc, chalk, quartz, attapulgite, montmorillonite, crushed and fractionated natural minerals such as calcite, marble, pumice, dextrin, precipitated silica, sepiolite, bentonite, river sand, white sand, zeolites, starch, sand, talc, quartz, dolomite, diatomaceous earth, aluminium oxide, silicates, calcium phosphates, calcium hydrogen phosphates, ammonium sulphate or mixtures thereof. Thickening agents are selected from the group comprising of, but not limited to, thickening agents by weight of the formulation, selected from Hydrophobic fumed silica, Polysaccharides / carboxymethyl cellulose / Bentonite Clay or Organic derivative of hectorite clay. The thickening agents is present in an amount of from ranges from 0.10 – 1.0% by weight based on the total weight of the composition. In an embodiment, the compositions of the present disclosure are produced by combining the actives with at least one agrochemically acceptable excipient to prepare a mixture and formulating the mixture into a solid or liquid formulation. Examples of the solid or liquid formulation includes, but are not limited to, , suspension concentrates (SC), emulsifiable concentrates (EC), Suspo emulsion (SE). According to an embodiment, the present disclosure provides a fungicidal composition comprising: a) at least one thiophosphate fungicide b) at least one antibiotic fungicide; c) at least one Triazole fungicide; and d) at least one agrochemically acceptable excipient. wherein the fungicidal composition is present in the form of a liquid formulation. In an embodiment, the liquid formulation is selected from Capsule suspension (CS), Dispersible concentrate (DC), Emulsion, water in oil (EO), Emulsion, oil in water (EW), Oil dispersion (OD), Oil miscible flowable concentrate (oil miscible suspension (OF), Oil miscible liquid (OL), Suspension concentrate (SC),Suspo-emulsion (SE) and (EC) Emulsion Concentrate. In a preferred embodiment, the liquid formulation is a suspension concentrate (SC), Emulsion Concentrate (EC), and Suspo-emulsion (SE). In another embodiment, the fungicidal combinations disclosed herein may be applied simultaneously as a tank mix or together as a pre-mix formulation, or each fungicide may be applied sequentially. The undesired pathogenic microorganism for the present invention is selected from the group comprising of Albugo spp. (white rust) on ornamentals, vegetables (e.g. A. Candida) and sunflowers (e.g. A. tragopogonis); Alternaria spp. (Alternaria leaf spot) on vegetables, citrus fruits (A. citri), rape (A. brassicola or A. brassicae), sugar beets (A. tenuis), fruits, rice, soybeans, potatoes (e.g. A. solani or A. alternata), tomatoes (e.g. A. solaniorA. alternata) and wheat; Aphanomyces spp. on sugar beets and vegetables; Ascochyta spp. on cotton, cereals and vegetables, e.g. A. tritici(anthracnose) on wheat and A. hordeion barley; BipolarisandDrechslera spp. (teleomorph: Cochliobolus spp. for e.g. Cochlioboluscarbonum(northern corn leaf blight)), e.g. Southern leaf blight (D. maydis) or Northern leaf blight (B. zeicola) on corn, e.g. spot blotch (B. sorokiniana) on cereals, e.g. B. oryzae on rice and turfs and on oats; Blumeria(formerly Erysiphe) graminis(powdery mildew) on cereals (e.g. on wheat or barley); Botrytis cinerea(teleomorph: Botryotiniafuckeliana: grey mold) on fruits and berries (e.g. strawberries), vegetables (e.g. lettuce, carrots, celery and cabbages), rape, flowers, vines, forestry plants and wheat; Bremialactucae(downy mildew) on lettuce; Ceratocystis (syn. Ophiostoma) spp. (rot or wilt) on broadleaved trees and evergreens, e.g. C. ulmi(Dutch elm disease) on elms; Cercospora spp. (Cercospora leaf spots) on corn and cotton, (Cercospora blight spots) on cotton, (e.g. Gray leaf spot: C. zeae-maydis), rice, sugar beets (e.g. C. beticola), sugar cane, vegetables, coffee, soybeans (e.g. C. sojinaorC. kikuchii) and rice, sunflower (e.g. cercospora leaf spot: C. helianthi),peanut (e.g. early leaf spot: C. arachidicola); Cercosporidium spp. on peanut (e.g. C. personatum: late leaf spot); Cladosporium spp. on tomatoes (e.g. C. fulvum: leaf mold) and cereals, e.g. C. herbarum (black ear) on wheat, C. caryigenum(pecan scab) on pecan; Cylindrocladium spp. on peanut (C.crotalariae: cylindrocladium black rot); Clavicepspurpurea(ergot) on cereals; Cochliobolus(anamorph: Helminthosporium of Bipolaris) spp. (leaf spots) on corn (C. carbonum), cereals (e.g. C.sativus(black point), anamorph: B. sorokiniana) and rice (e.g. C. miyabeanus, anamorph: H. oryzae);Colletotrichum (teleomorph: Glomerella) spp. (anthracnose) on cotton (e.g. C. gossypii), corn (e.g. C.graminicola: Anthracnose stalk rot), soft fruits, potatoes (e.g. C. coccodes: black dot), beans (e.g. C.Iindemuthianum), citrus fruits (e.g. C. acutatum(post bloom fruit drop), C. gloeosporioides) andsoybeans (e.g. C. truncatumorC. gloeosporioides); Corticium spp., e.g. C. sasakii(sheath blight) onrice; Corynesporacassiicola(leaf spots) on soybeans and ornamentals; Cycloconium spp., e.g. C.oleaginumon olive trees; Cylindrocarpon spp. (e.g. fruit tree canker or young vine decline,teleomorph: NectriaorNeonectria spp.) on fruit trees, vines (e.g. C. liriodendri, teleomorph:Neonectrialiriodendri: Black Foot Disease) and ornamentals; Dematophora(teleomorph: Rosellinia)necatrix(root and stem rot) on soybeans; Diplodia spp. e.g. Diplodia boll rot on cotton, Diaporthespp., e.g. D. phaseolorum(damping off) on soybeans, D. citri(melanose) on citrus fruits; Drechslera (syn. Helminthosporium, teleomorph: Pyrenophora) spp. on corn, cereals, such as barley (e.g. D.teres, net blotch), oats (e.g. D. avenae, leaf spot), and wheat (e.g. D. tritici-repentis: tan spot), rice andturf; Esca (dieback, apoplexy) on vines, caused by Formitiporia(syn. Phellinus) punctata, F.mediterranea, Phaeomoniellachlamydospora (earlier Phaeoacremoniumchlamydosporum), Phaeoacremoniumaleophilumand / or Botryosphaeriaobtusa; Elsinoe spp. on pome fruits (E. pyri),on citrus fruits (E. fawcetti), soft fruits (E. veneta: anthracnose) and vines (E. ampelina: anthracnose);Entyloma oryzae (leaf smut) on rice; Epicoccum spp. (black mold) on wheat; Erysiphe spp. (powderymildew) on sugar beets (E. betae), (powdery mildew) on rye (E. graminis), vegetables (e.g. E. pisi),such as cucurbits (e.g. E. cichoracearum), cabbages, sunflower, rape 5 (e.g. E. cruciferarum), peas andbean (e.g. E. polygoni); Eutypalata(Eutypa canker or dieback, anamorph: Cytosporinalata, syn.Libertellablepharis) on fruit trees, vines and ornamental woods; Exserohilum (syn.Helminthosporium) spp. on corn (e.g. E. turcicum); Fusarium (teleomorph: Gibberella) spp. (wilt,root or stem rot) on various plants, such as hardlock, boll rot of cotton, F. graminearumorF.culmorum(root rot, scab or head blight) on cereals (e.g. wheat or barley), F. oxysporumontomatoes,F. solanion soybeans and F. verticillioides on corn; Gaeumannomycesgraminis(take-all) on cereals(e.g. wheat or barley) and corn; Gibberella spp. on cereals (e.g. G. zeae) and rice (e.g. G. fujikuroi:Bakanae disease); Glomerellacingulataon vines, pome fruits and other plants and G. gossypiioncotton; Grain staining complex on rice; Guignardiabidwellii(black rot) on vines, Guignardiacitricarpa(balck spot) on citrus fruits; Gymnosporangium spp. on rosaceous plants and junipers, e.g. G. sabinae(rust) on pears; Helminthosporium spp. (syn. Drechslera, teleomorph: Cochliobolus) oncorn, cereals and rice; Hemileia spp., e.g. H. vastatrix(coffee leaf rust) on coffee; Isariopsis clavispora(syn. Cladosporium vitis) on vines; Kabatiellazeae(eyespot) on corn; Leptosphaeria maculans(blackleg) on oilseed crops; Leptosphaerulina spp. on peanut (e.g. L. crassiasca:pepperspot); Macrophominaphaseolina(syn. phaseoli) (root and stem rot) on soybeans and cotton; Microdochium (syn. Fusarium) nivale (pink snow mold) on cereals (e.g. wheat or barley);Microsphaeradiffusa(powdery mildew) on soybeans; Myeosphaerellacitri(greasy spot) on citrusfruit, Monilinia spp., e.g. M.nodorum(Stagonospora blotch, teleomorph: Leptosphaeria (syn. Phaeosphaeria) nodorum) on wheat, septrotialeaf and glume blotch on rye; Stemphyllium spp. e.g. stemphyllium leaf spot on cotton, Synchytriumendobioticumon potatoes (potato wart disease); Taphrina spp., e.g. T. deformans (leaf curl disease)on peaches and T. pruni(plum pocket) on plums; Thielaviopsis spp. (black root rot) on tobacco, pomefruits, vegetables, soybeans and cotton, e.g. T. basicola(syn. Chalara elegans); Tilletia spp. (commonbunt or stinking smut) on cereals, such as e.g. T. tritici(syn. T. caries, wheat bunt) and T. controversa(dwarf bunt) on wheat; Typhulaincarnata(grey snow mold) on barley or wheat; Urocystis spp., e.g.U. occulta (stem smut) on rye; Uromyces spp. (rust) on vegetables, such as beans (e.g. U.appendiculatus, syn. U. phaseoli) and sugar beets (e.g. U. betae); Ustilago spp. (loose smut) oncereals (e.g. U. nuda and U. avaenae), corn (e.g. U. maydis: corn smut) and sugar cane; Venturia spp.(scab) on apples (e.g. V. inaequalis) and pears; and Verticillium spp. (wilt) on various plants, such asfruits and ornamentals, vines, soft fruits, vegetables and field crops, e.g. V. dahliaeonstrawberries,rape, potatoes and tomatoes. In yet another embodiment, the combination or the composition of the present invention is effective for management of fungi or pests selected from one or more of. wheat, rye, barley, triticale, oats or rice; beet, e.g. sugar beet or fodder beet; fruits, such as pomes, stone fruits or soft fruits, e.g. apples, pears, plums, peaches, almonds, cherries, strawberries, raspberries, blackberries or gooseberries; leguminous plants, such as lentils, peas, alfalfa or soybeans; oil plants, such as rape, mustard, olives, sunflowers, coconut, cocoabeans, castor oil plants, oil palms, ground nuts or soybeans; cucurbits, such as squashes, cucumber or melons; fiber plants, such as cotton, flax, hemp or jute; citrus fruit, such as oranges, lemons, grapefruits or mandarins; vegetables, such as spinach, lettuce, asparagus, cabbages, carrots, onions, tomatoes, potatoes, cucurbits or paprika; lauraceous plants, such as avocados, cinnamon or camphor; energy and raw material plants, such as corn, soybean, rape, sugar cane or oil palm; corn; tobacco; nuts; coffee; tea; bananas; vines (table grapes and grape juice grape vines); hop; turf; sweet leaf (also called Stevia); natural rubber plants or ornamental and forestry plants, such as flowers, shrubs, broadleaved trees or evergreens, e.g. conifers; and on the plant propagation material, such as seeds, and the crop material of these plants. In yet another preferred embodiment, the present invention provides a fungicidal combination or composition comprising of Iprobenfos, Validamycin, and Tebuconazole to control the pathogenic microorganism on economically important crops such as rice, chilli, apple, peppers, soybean, cotton, chick pea, pigeon pea, Grapes, Apple and pomegranate, tea, potato, and tomato. In another embodiment, the present invention provides a combination / composition that shows enhanced action against undesired pathogenic microorganisms, in comparison to the control rates that are possible with the individual compounds and / or suitable for improving the health of plants when applied to plants, parts of plants, plant propagation materials, or at their locus of their growth. SInEasT E 1r.axmh x0 9 8 7 6 5 4 3 2 1Nn amea o S.maem e pn xl yamp l Bil A T r T b V oe1 vpeseonP x mis Xz aBtyebualDiiIpd im:Parisro nina le:s pe loe ryco drao tmbI enreio bMan othyPocksallph n a yen ngtpa n selowthaia lenlyco t oenzocifore,thra thwanteG zoeGalkpofpo llensrulinlT T T dieectiey yr al lyhEothe ecech nth oneorem -3enm ch hn sem oofg-conl eoerspo h xy n n ii icaic f a ive x idat la cacal l a fureee te llc penon m g o is soQc sile1 % ploid b y.S0 .10 .15 0 .22 3 9 .02 .57wE gsali l ewfoto0 0.00.0.00 0.50 / w 1tio crnomith tohof p t o uepQS1 %hs tdu.0 0 5 0 2 3 5 3 0 Ee it e rpt.10.10.0.20.0.0.00.00.0 wg 2p io poo0 / wresn eaartiseoQn.S0 0 0 5 1 w Et snSgfito.10.15 0.0.22 0.03 9 .0.00.05 0.00 / w%gf3 uufnr llugsp oQ %iciem nstthrat.S0 .10 .15 0 2 . 2 3 0 2 . 5 .wEdsg a ioeiosn to0 0.0 20.0.0.0 0 00 0 0 / w 4l isn dcpoir anQ 1 % Eepnit d ceana.S0 0 5 0 2 3 5 5 0icndreto.10.10.0.20.0.0.00.00.0 wg 0 / w 5tedtr basc no teopttoQ %el.S0 to.10 0.15 0 2 5 Eows(eo b0.0.22 0.03 5 .0.00.50.00wg / w 6iSnCftecT)h oa: eQ.S0 1 % bin nsto.10 0.15 0 E vtru0.0.22 0.03 5 3 .0.00.05 0.0 wgle0 / 1enedw 7: tionasQl.S0 1 1 % to.10 E.im0.15 0 0.0.22 0.03 .05 5 .0.00 0 0.0 wgi0 / w 8tatio% n Q 0 0 0 1 5 Es.S5 2 3 5 5w oto.10.10.0.20.0.0.0.0.0g 0 0 0 / w 9fthe Q.S0 to.10 0.15 0 0.0.22 3 5 1 0.0.0.00 1 % 0.00 Epwgres 0.00 / w 10entinv ention , Process for preparing Suspension concentrates (SC)formulation: 1) A required quantity of water, biocide, and defoamer is charged into a mixing vessel. Gum powder is added gradually with continuous stirring to obtain a gum solution. The gum solution is aged for 12–18 hours prior to use to achieve uniform hydration and desired viscosity. 2) In a separate vessel, the required quantity of demineralized (DM) water is charged, followed by sequential addition of wetting agent, dispersing agent, and suspending agent. The mixture is homogenized using a high shear homogenizer for 60–90 minutes to obtain a uniform slurry. 3) The technical active ingredient(s) and other remaining adjuvants (excluding antifreeze and thickeners) are added to the homogenized slurry obtained in Step 2, resulting in a uniform slurry composition. 4) Half of the required quantity of antifoam agent is added to the slurry. The resulting mixture is subjected to particle size reduction using appropriate equipment such as a Dyno-Mill until particles of the desired size are achieved. 5) The remaining half of the antifoam agent along with the antifreeze agent is incorporated into the processed slurry from Step 4. 6) The gum solution prepared in Step 1 is added to the mixture obtained in Step 5 to yield the final suspension concentrate formulation.

[0002] 1 1 9 8 7 6 5 4 3 2 N S T E2 0 1 o .r. ab x l a e m B A e S E XnrzotPilo hT oebV aIp 2p :FleDaMnism oa rox B ltpox T u ylr cio dryanckphla istnaomb u2enInn:g gPwthuthicmiaz Sloenoxeipdocootse yp Aryazoy cinfosredicird epater G olveGe lypo h ulinenlyallyaml-pleteinh eT T Tie a a ee ecnlCram -3t-o Cckoy mel leersna o chch hts lln n n icoti mon n -I eX net oic ialca a llp f ooQs f.S0 .10 .115 5 0 9 2 1 .0.21 .02 .0.0.57 w EitF. / %gioun n g to0 0.00 0 050w 1 oiciQ 0 0 1 w Eft dha.S.1.115 5 0 .21 2 5 .03 .00 to0 0.0.00.0.00 0.00 / w%g 2eSlcuom Q Espp.S0 .10 .115 5 0 .21 2 9 .05 .05 .0w %g o o to0 0.0.00.0.00 0 0 / w 3 EsmitioQ.S0 n .10 .115 5 0 .21 2 5 . 2 . 5 . w % E u glao.t0 0.0 00.0.0 000000 / w 4sionsSu Q.S0 .10 . 15 5 0 1 . 1 2 5 5 0 w % E (Ssg Epo to010.0.0 20.0.0.00.00.00 / w 5)FeomQr.S mu %lsito0 0 1 0 5 2 5 E uonm.10.15 5 0. .0.21 .02 .0.0.5.0 wglaa00 0 0 06ti (Sk / w on Ee . )FQ o.S%rmto0m.10 0.115 5 0 0.a0.0.21 2 5 0.0.0.03 1 0.05 0.0 wE u gl0k / aw 7tioen : Q.Sto1 % 0m.10 1 1 5 5 0 1 2 5 5 0 0.10.a0.0.20.0.0.0.0 wE g 0 0.00k / w 8eQ.S% to0m.10 0.115 5 0 1 0.0.0.21 2 5 5 0.0.0.0.05 0.0 wE ga0 09k / weQ.Sto0 .10 . 15 5 0 . 1 2 5 1 1 % E . 0 0wgm010.0.0 20.0.0 00.0.0 1a0 0k / w0e Ta S b 12 0 9 8 7 6 5 4 3 2 1r le1.N 3o:.T ristySryB Ailol-pT V erx heba IpXnzom P ranBenulidroDaMnistoaohthticpeyploocoaockphlEnmb enIngwumiaSloenlycoo spthaz yfooci osreaz dter G oul live aleG kp y o h xlen TlyatyT Te ienmn-n3tClycolenmela e ec chte tech hs n n -on -Il eeX o r An x mic ic icaidia enel al lsalt of Su spA o n t W D A A A -i-DiEespctictictim F C Th B Sfr tteva eveve u u ir ioo eeefinrsI I Inlsirickernci edlvrezi oentnagmgiA ngngngngctoin Aer g eArerereo(Sngdid dn eieie E g ent en n n n)t t t t tQ.Stom1 Q 0 .10 .115 5 0 .0.21 .02 9 2 .0.0.57 . Atyak0 0.00 0 050 (eg1)00 Q.SQto0 .10 .115 5 0 .0.21 . 2 1 . 5 5 1 ..00 . Btym0 0.000 0 00 000 (agk)eQ.SQ to0 .10 .115 5 0 .0.21 .02 15 5 .05 .0Ctym0 0.00.0.00 0 0 (agk)e Process for preparing Suspo Emulsion (SE) Formulation. 1) A required quantity of water, biocide, and defoamer is charged into a mixing vessel. Gum powder is added with continuous stirring to form a gum solution. The gum solution is allowed to stand for 12–18 hours prior to use for proper hydration. 2) Separately, the required quantity of demineralized (DM) water is charged into a vessel. Wetting agent, dispersing agent, and suspending agent are sequentially added and homogenized using a high shear homogenizer for 60–90 minutes to obtain a uniform slurry. 3) The technical active ingredient(s) and other remaining adjuvants (excluding antifreeze and thickeners) are added into the homogenized slurry of Step 2 to obtain a uniform pre‑slurry mixture. 4) Half of the required antifoam agent is added to the slurry. The resulting mixture is subjected to particle size reduction using equipment such as a Dyno‑Mill until particles of the desired size are achieved. 5) The remaining half of the antifoam agent together with the antifreeze agent is incorporated into the processed slurry. Finally, the gum solution prepared in Step 1 is added to form the final suspo‑emulsion formulation.

[0003] 2 1 noSTa E 7 6 5 4 3 x .r.b leam HeavfoD M FanalkT4d y VIn:p Fle3yrmaiamixtty ElarC M Tei ebT ue aliTIpde rog un:Proamemit xyAth yolsalcxchituncoc nhnac mhn breedg ire dhylenlcoxyupuh mre icaazica yicnafoiencid pa aatice l eholaoo l o lcilte n ls ts d nf l raaleCtteo i mon Qo.S1 2 1 9 .02 1 % p . 7wE o gs fF.0to.00 .02 .0050.50 / w 1itioun n g oiciQd1 2 1 1 %fEtha.S0.to.00 .02 5 .0.03 0.00 0.0 wge0 / w 2 Elcmom u p Qlso %s.S10 20 12 9 5 5wEifit gia io.to.0.0.0.00.00.00 / w 3b l n eC aQ os.S1 %En m 0 20 1 5 2 5 Ecu.to.0.02 .0.00.00.00wg e / w 4 ntlrsaiftiab Q 1 %e(l.S10 20 12 5. .05 .00wE gEecoto.0.0.00 0.0C 0 / w 5)Fn oce rnQ %mtr E ua.S1.0 2 1 5 2 5tto.00 .02 .0.0e0.50.00wgla / w 6ti(o En C .)Q 1 2 1 1 %FE o.S.0to.00 .02 5 3 5 .0.00.00.0 wgrm0 / w 7u latioQ % n.S1 1 1 0 20 1 5 5 0 E:. . .2.0.0.0 wgto 0 0.00 0 0 / w 8Q.S1 2 1 1 % 5 5 5 E.0to.00 .02 .0.0.00 0.00wg / w 9Q.S1 1 1 % E 0 20 12 5 0 0 g.to.0.0.0.00.00.0 w0 / w 10 Process for preparing Emulsifiable concentrate (EC) Formulation. 1) A required quantity of heavy aromatic solvent, dimethylformamide, and mixed xylene is charged into a mixing vessel. The required quantity of emulsifiers is added into the mixture, followed by homogenization using a high shear homogenizer for a period of 60–90 minutes to obtain a uniform homogenized solvent‑emulsifier mixture. 2) The technical active ingredients—Iprobenfos, Validamycin, and Tebuconazole—are added sequentially into the homogenized solvent– emulsifier mixture obtained in Step 1 and stirred until a uniform emulsion concentrate solution is achieved. 3) The prepared emulsion concentrate is subjected to quality control tests in accordance with specified parameters. After successful qualification, the product is filtered (if required) and filled into desired pack sizes. Example 4 Evaluation of Bio-efficacy & Phytotoxicity of Fungicidal Composition and thereof (Iprobenfos and Validamycin and Tebuconazole SE against Sheath Blight disease on Rice crop. Methodology The experiment was conducted at Warangal during Rabi 2024-25, to study the efficacy of Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE against sheath blight disease of rice. All the standard package of practices of Rice from sowing to harvesting stage of the crop was followed uniformly in all the treatments. A minimum plot size of 25 m² per replication per treatment was maintained. The treatments were imposed immediately after the first appearance of the disease was done and the spraying with water was kept as untreated check as control. An observation on the percentage disease incidence of the Sheath blight disease phytotoxicity effect of the crop and yield -kg / ha was recorded as per the treatments given. Details of the Experiment: Crop Rice Variety WGL-962 Soil type Black cotton soils Season & Year Rabi, 2024-25 Date of sowing 08-1-2024 Replications 03 Treatments 10 Plot size 25 m2(5 x 5 m) per replication per treatment Spacing 15 x 10 cm Design Randomized Block Design (RBD) Stage of application of treatments Treatments were imposed immediately after first appearance of the sheath blight disease (Max.Tillering stage) Date of treatments application 25.3.2025 Observations recorded 1. Percent disease incidence of Sheath blight disease 2. Phytotoxicity 3. Yield data Date of harvesting 28.4.2025

[0004] Table 5: Treatment details: Dose / ha Treatment no. Treatment Details a.i (g) Formulation (gm or ml) Iprobenfos 17.5% + Validamycin 2.5% + T1 217.5 750 g / ha Tebuconazole 9% SE Iprobenfos 17.5% + Validamycin 2.5% + T2 290 1000g / ha Tebuconazole 9% SE Iprobenfos 17.5% + Validamycin 2.5% + T3 362.5 1250g / ha Tebuconazole 9% SE T4 Iprobenfos 48% EC 240 500 ml / ha T5 Validamycin 3% L 60 2000ml / ha T6 Tebuconazole 38.39%w / w SC 215 500 ml / ha T7 Hexaconazole 5.00% + Validamycin 2.50%SC 75 1000ml / ha T8 Control (Untreated Check) Table 6. Effect of Iprobenfos + Validamycin + Tebuconazole SE against Sheath Blight disease of Rice. Percentage disease Incidence of Treatment AI / ha sheath blight of Rice (%) Details of the Treatment no. (gms) Before 15 DAS ROC Spray Iprobenfos 17.5% + Validam 15.69 26.22 T1 ycin 217.5 55.11 2.5% + Tebuconazole 9% SE -22.96 -30.75 Ip 13.45 15.58 T2 robenfos 17.5% + Validamycin 290 73.33 2.5% + Tebuconazole 9% SE -20.85 -22.92 Iprobenfos 17 22.65 14.34 T3 .5% + Validamycin 362.5 75.45 2.5% + Tebuconazole 9% SE -28.31 -22.23 28.84 27.77 T4Iprobenfos 48% EC24052.45 -32.33 -31.28 10.96 36.48 T5Validamycin 3% L6037.54 -18.95 -37.13 21.11 39.95 T6Tebuconazole 38.39%w / w SC21531.6 -27.31 -38.86 Hexacon 17.25 33.2 T7 azole 5.00% + 75 43.17 Validamycin 2.50%SC -24.52 -34.84 37.9 58.41 T8Control (Untreated Check) 0 -37.92 -49.83 Results: Percent Disease Incidence of Sheath Blight in Rice Sheath blight disease symptoms were observed in the field 50 days after transplanting, and all treatments were applied immediately after disease appearance. Percent disease incidence was recorded 15 days after spraying across all treated and control plots, with values ranging from 14.34% to 58.41%. The lowest disease incidence (14.34%) was recorded in T3 – Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE @ 362.5 g a.i / ha, followed by T2 – Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE @ 290 g a.i / ha with 15.58% disease incidence. The highest incidence (58.41%) was recorded in the untreated control. Reduction Over Control (ROC) • T3 recorded the highest ROC (75.45%), followed by T2 with 73.33%. • Both T2 and T3 were statistically at par and significantly superior to all other treatments tested. Table 7: Impact of Iprobenfos + Validamycin + Tebuconazole SE on the yield of rice crop. Treatment AI / ha Yield Details of the Treatment no. (gms) (Kg / ha) % Yield IncreaseIprobenfos 17.5% + Validamycin 2.5% T1 217.5 7160 47% + Tebuconazole 9% SE Iprobenfos 17.5% + Validamycin 2.5% T2 290 8390 73% + Tebuconazole 9% SE Iprobenfos 17.5% + Validamycin 2.5% T3 362.5 8450 74% + Tebuconazole 9% SE T4 Iprobenfos 48% EC 240 7350 51% T5 Validamycin 3% L 60 6725 38% T6 Tebuconazole 38.39%w / w SC 215 6525 34% Hexaconazole 5.00% + Validamycin T7 75 6850 41% 2.50%SC T8 Control (Untreated Check) 4853 Results: Grain Yield The highest grain yield (8,450 kg / ha) was recorded in T3 – Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE @ 362.5 g a.i / ha (1.25× dosage), corresponding to a 74% increase over the untreated control. This was followed closely by T2 – Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE @ 290 g a.i / ha, which yielded 8,390 kg / ha with a 73% increase over control. Both T2 and T3 were economically at par and significantly superior to all other treatments tested. Phytotoxicity Observation: Phytotoxicity was assessed by visual observation. Ten plants in each treatment per replicate were observed critically at 1, 3, 7, 10 and 14 days after spraying for chlorosis, leaf tip injury, necrosis, epinasty, hyponasty, vein clearing and wilting and were graded on 0–10-point phytotoxicity scale. Crop response / crop injury Grade 0-00 0 1-10% 1 11-20% 2 21-30% 3 31-40% 4 41-50% 5 51-60% 6 61-70% 7 71-80% 8 81-90% 9 91-100% 10 Example 5: Phytotoxicity effect of Iprobenfos + Validamycin + Tebuconazole SE on Rice crop. Table 8. Phytotoxicity effect in Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE at (T2: 290 a.i g / ha) on different days after treatment during Rabi, 2024-25 Days after treatment Rating scale Score 1 0 No phytotoxicity 3 0 No phytotoxicity 5 0 No phytotoxicity 7 0 No phytotoxicity 10 0 No phytotoxicity Table 9. Phytotoxicity effect in Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE at (T3: 362.5 a.i g.ha) on different days after treatment during Rabi, 2024-25. Days after treatment Rating scale Score 1 0 No phytotoxicity 3 0 No phytotoxicity 5 0 No phytotoxicity 7 0 No phytotoxicity 10 0 No phytotoxicity Result: Phytotoxicity Effect of Iprobenfos + Validamycin + Tebuconazole SE Data on various phytotoxicity parameters are presented in Tables 2, 3, and 4. The results from the experiment revealed that the application of Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE on rice showed no phytotoxic effects at the tested doses for T2, T3, and untreated control. The product was found to be safe for the crop. None of the tested treatments exhibited any phytotoxic symptoms—such as yellowing, stunting, necrosis, epinasty, or hyponasty—at 1, 3, 5, 7, and 10 days after spraying. Conclusion Among all the treatments, T2 (290 g a.i / ha) and T3 (362.5 g a.i / ha) containing Iprobenfos 17.5% + Validamycin 2.5% + Tebuconazole 9% SE performed best against sheath blight disease of rice compared to the other treatments. Hence, this fungicide combination can be effectively used for the management of sheath blight in rice.

Claims

1. We Claims:

1. A fungicidal composition comprising a synergistic combination of- a. Iprobenfos is present in an amount of 5% to 17.50% by weight; b. Validamycin is present in an amount of 2% to 10% by weight; c. Tebuconazole is present in an amount of 5% to 20% by weight, and d. Agrochemical acceptable excipient.

2. The fungicidal composition as claimed in claim 1, wherein Iprobenfos is present in an amount of 17.50% w / w, Validamycin is present in an amount of 2.5% w / w, and Tebuconazole is present in an amount of 9% w / w.

3. The fungicidal composition as claimed in claim 1, wherein the agrochemically acceptable excipient is Emulsifiers, dispersant / dispersing agents, carriers, thickeners / binders, Biocides, wetting agents, antifreeze agents, antifoaming agents, carriers, and solvents.

4. The fungicidal composition as claimed in claim 1, wherein the formulation is selected from Suspension concentrate (SC), suspo-emulsion (SE), and Emulsion Concentrate (EC).

5. The fungicidal composition as claimed in claim 4, wherein the SE comprises at least a dispersing agent in an amount in the range of 2-6%w / w, at least a wetting agent in an amount in the range of 1-4%w / w, at least an anti-foaming agent in an amount in the range of 0.10-10%w / w, at least an anti-freezing agent in an amount up to 3- 10%w / w, at least Biocide agent in an amount in the range of 0.10-1.0%w / w, at least a thickening agent in an amount in the range of 0.10-50%w / w and a solvent up to 95%w / w.

6. The fungicidal composition as claimed in claim 5, comprising Tristyryl-phenol Ethoxylate Amine salt of phosphate of 2%, Block copolymer of 1%, Siloxane poly alkylene oxide of 0.20%, Propylene Glycoof 5%, Aromatic Solvent C-IX of 15%, Benzisothiazolin-3-one of 0.10%, Xanthum Gum of 0.10%, Distilled water to make up to 100%w / w.

7. The fungicidal composition as claimed in claim 4, wherein the SC comprises at least a dispersing agent in an amount in the range of 2-6%w / w, at least a wetting agent in an amount in the range of 1-4%w / w, at least an anti-foaming agent in an amount in the range of 0.10-10%w / w, at least an anti-freezing agent in an amount up to 3- 10%w / w, at least Biocide agent in an amount in the range of 0.10-1.0%w / w, at least a thickening agent in an amount in the range of 0.10-50%w / w, at least an solvent agent in an amount up to 1-15%w / w, and a carrier up to 95%w / w.

8. The fungicidal composition as claimed in claim 7, comprising Tristyryl-phenol Ethoxylate Amine salt of phosphate of 3%, Block copolymer of 2%, Siloxane poly alkylene oxide of 0.20%, Propylene Glycol of 5%, Benzisothiazolin-3-one of 0.10%, Xanthum Gum of 0.10%, Distilled water to make up to 100%w / w.

9. The fungicidal composition as claimed in claim 4, wherein the EC comprises at least a emulsifier agent in an amount in the range of 2-6%w / w, at least a carrier up to 95%w / w.

10. The fungicidal composition as claimed in claim 9, comprising - Mixture of Calcium alkylarylsuphonate and Ethoxylated Fatty Alcohol of 12%, Mix xylene of 20%, Dimethyl formamide of 10%, Heavy aromatic solvent to make up to 100%w / w.

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