Device for detecting at least one component present in a fluid and corresponding method

Abstract

The invention relates to a stand-alone device for detecting the presence of at least one component present in a fluid, which device comprises: - a chemical reaction module (10) provided with a reactor (11), and means for controlling the temperature of the reactor and of a magnetic trap (12); - a main circuit for circulating at least one fluid through the reactor and the magnetic trap; - at least one main pump (18) which, when in use, circulates at least one fluid in the main circulation circuit; - at least one tank (35) of magnetic beads associated with a first metering pump for injecting magnetic beads, in doses, into the main circuit; - at least one tank of at least one reagent associated with a second metering pump for injecting the reagent, in doses, into the main circuit; - a member for controlling at least the main pump, the first metering pump, the second metering pump, the temperature control means and the magnetic trap. The invention further relates to a corresponding detection method.

Description

[0001] DEVICE FOR DETECTING AT LEAST ONE COMPONENT PRESENT IN A FLUID AND CORRESPONDING METHOD

[0002] The invention relates to a device for detecting at least one component present in a fluid.

[0003] The invention also relates to a method for detecting at least one component present in a fluid implemented by means of such a device.

[0004] BACKGROUND OF THE INVENTION

[0005] The bacteriological quality of water intended for human use is today essentially assessed and controlled from a regulatory point of view by the search for indicators of fecal contamination or specific pathogens.

[0006] Primarily, a sample of the water to be studied is taken and then a culture of the bacteria present in the sample is carried out.

[0007] This technique allows the search for specific bacteria but requires a minimum of 36 hours to establish a diagnosis of contamination, in the case of indicators of fecal contamination, and more than three days for the search for specific pathogens (Legionella, Vibrio, etc.).

[0008] These delays are mainly due to the unavoidable culture times for the development of the bacteria sought and the colony identification procedures.

[0009] Such a technique is therefore too time-consuming to establish a rapid and / or regular diagnosis of contamination in a given environment. Furthermore, the use of selective practices in culture limits the development of the organisms being sought and, in many cases, leads to an underestimation of their actual abundance.

[0010] Molecular approaches used in conjunction with or independently of culture techniques reduce diagnostic delays, thus providing access to faster and more sensitive methods. These approaches rely in particular on gene amplification techniques, which allow for the measurement of the abundance of a genus or species by amplifying a phylogenetic gene.

[0011] However, although effective, these molecular approaches are not very attractive for high-frequency monitoring because of their cost of analysis, the technicality required to implement them and because they do not provide information, when used independently of culture, on the active part of the bacteria detected.

[0012] The idea was to work instead on techniques borrowed from molecular methods, particularly sandwich hybridization techniques using DNA probes and nucleic acid targets, known as genosensors. These techniques allow for the quantification of bacterial contaminants in a sample without a prior culture step. Bacterial detection is achieved through the capture and dual recognition of a fragment of its genetic material that is specific to the bacterium, and the concentration of this fragment can be correlated with the abundance of bacteria present in a sample.

[0013] Unfortunately, this type of technique currently requires the intervention of extremely qualified personnel, which is not economically suitable for large-scale production and / or widespread application in monitoring programs dedicated to aquaculture and environmental security.

[0014] SUBJECT OF THE INVENTION

[0015] The invention aims in particular to facilitate the detection of at least one component present in a fluid, the fluid itself being present in a given environment.

[0016] SUMMARY OF THE INVENTION

[0017] To this end, a device is provided for detecting at least one component present in a fluid, the device comprising a chamber in which at least:

[0018] - a chemical reaction module equipped with a reactor, a magnetic trap and means for thermal regulation of the chemical reaction module,

[0019] - a main circulation circuit of at least one fluid through the reactor and the magnetic trap,

[0020] - at least one main pump ensuring, in operation, the circulation of at least one fluid in the main circulation circuit,

[0021] - at least one reservoir of magnetic beads associated with a first dosing pump for injecting doses of magnetic beads into the main circuit,

[0022] - at least one reservoir of at least one reagent associated with a second dosing pump for injecting the reagent into the main circuit in doses,

[0023] - a control system for at least the main pump, the first metering pump, the second metering pump, the thermal regulation means and the magnetic trap.

[0024] Thus, the device proves to be autonomous in that it can operate without operator intervention once installed in a given environment. Furthermore, it can be installed on-site by even an unqualified operator.

[0025] Advantageously, the invention can be used in any environment, whether natural or industrial.

[0026] Moreover, the invention proves to be relatively inexpensive to produce.

[0027] Optionally, the main pump is a peristaltic pump.

[0028] Optionally, at least one of the metering pumps is a solenoid pump.

[0029] Optionally, the chemical reaction module includes at least one static mixer, the main circuit being arranged to allow the fluid to circulate in a loop between the reactor and the mixer.

[0030] Optionally, the device includes means for maintaining the reagent reservoir at a set temperature.

[0031] Optionally, the support means include a Peltier effect cell.

[0032] Optionally, the device includes a secondary circuit connected to the main circuit, the secondary circuit also extending into the casing.

[0033] Optionally, the secondary circuit includes a second pump.

[0034] Optionally, the secondary circuit includes at least one analysis module. Optionally, the analysis module includes a colorimeter.

[0035] The invention also relates to a method for controlling a fluid using a device such as the one mentioned above, the method comprising the steps of:

[0036] - Introduce at least one fluid into the main circuit,

[0037] - Inject at least one dose of at least one reagent into the main circuit,

[0038] - Inject at least one dose of magnetic beads into the main circuit,

[0039] - Heat the mixture of fluid, beads and reagent in the reactor.

[0040] - Trap the magnetic beads in the magnetic trap in order to analyze the components trapped on the beads.

[0041] Other features and advantages of the invention will become apparent from the following description. This description presents a particular, but not limiting, embodiment of the invention.

[0042] BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Reference will be made to the attached drawings, among which:

[0044] [Fig. 1] is a perspective view of an autonomous detection device according to a particular embodiment of the invention;

[0045] [Fig. 2] is a schematic view of the device illustrated in Figure 1; [Fig. 3a] schematically illustrates different phases of a first stage of detection of at least one component present in a fluid by the device shown in Figure 1;

[0046] [Fig. 3b] schematically illustrates different phases of a second stage of detection of at least one component present in a fluid by the device shown in Figure 1;

[0047] [Fig. 3c] schematically illustrates different phases of a third stage of detection of at least one component present in a fluid by the device shown in Figure 1;

[0048] [Fig. 3d] schematically illustrates different phases of a fourth step of detection of at least one component present in a fluid by the device shown in figure 1;

[0049] [Fig. 3e] schematically illustrates different phases of a fifth step of detection of at least one component present in a fluid by the device shown in figure 1;

[0050] [Fig. 3f] schematically illustrates different phases of a sixth step of detection of at least one component present in a fluid by the device shown in figure 1;

[0051] [Fig. 3g] schematically illustrates different phases of a seventh step of detection of at least one component present in a fluid by the device shown in Figure 1;

[0052] [Fig. 3h] schematically illustrates different phases of an eighth step of detection of at least one component present in a fluid by the device shown in Figure 1.

[0053] DETAILED DESCRIPTION OF THE INVENTION

[0054] With reference to Figures 1 and 2, an autonomous device 1 for detecting at least one component present in a fluid according to a particular embodiment of the invention will now be described.

[0055] Device 1 comprises a casing 2. The casing 2 has a base from which one or more sides extend, forming a box open at the top. The casing 2 also has a lid 3 suitable for temporarily closing the box. The lid 3 is, for example, pivotally mounted on the box, or it can be temporarily attached to the box by clips, hooks, or any other fastening device. The lid 3 is optionally equipped with a handle 4 for handling.

[0056] The box 2 also includes at least one intake pipe opening outside the box 2.

[0057] The interior of box 2 will now be described, all the elements mentioned below being fully arranged in box 2.

[0058] Device 1 contains at least one power supply system for Device 1. The power supply system includes, for example, a battery 6. In this way, Device 1 can be recharged when it is returned to the laboratory before being sent out for a new measurement session. For this purpose, the housing 2 has a mains power connection and / or the battery 6 is removable so that it can be taken out of the housing 2 during recharging. The battery 6 is, for example, a lithium-ion battery.

[0059] Furthermore, device 1 includes at least one control system 7 for device 1. The control system 7 includes, for example, at least one calculator, a computer, etc.

[0060] Preferably, device 1 includes a wireless communication interface 8 with the outside. This communication interface 8 is housed in the enclosure 2. For example, this communication interface 8 is a Wi-Fi communication interface. For example, this communication interface 8 is integrated into the control system 7. This communication interface 8 thus allows device 1 to communicate with the outside without having to act directly on the enclosure 2.

[0061] Preferably, the device 1 includes at least one human / machine interface 9. Preferably, said human / machine interface 9 is arranged in the enclosure 2. Preferably, the human / machine interface 9 is configured so as to allow an operator to act on the device 1 by transmitting their orders, for example, to the control system 7. The human / machine interface 9 is, for example, a touch screen.

[0062] An operator can therefore interact with device 1 either via this interface or via the communication interface 8. The human / machine interface 9 and the communication interface can be common or can be separate.

[0063] In the present case, device 1 comprises several multi-way connection systems (which will be described below). Optionally, these connection systems are controlled by the control system 7. For example, this system includes control relays for these connection systems, all the relays being arranged on the same block, itself arranged within the housing 2. At least one of the connection systems is, for example, a solenoid valve.

[0064] Furthermore, at least part of the connection systems here are three-way:

[0065] - In a first configuration, a first track is in communication only with a second track, and the system then draws a first traffic path,

[0066] - In a second configuration, the first track is in communication only with a third track, and the system thus creates a second traffic path.

[0067] - in all cases the second track and the third track are not in communication with each other.

[0068] Device 1 also includes a chemical reaction module 10.

[0069] This chemical reaction module 10 includes a reactor 11 associated with a magnetic trap 12. The reactor 11 is for example made of or based on polytetrafluoroethylene.

[0070] The magnetic trap 12 includes, for example, at least one magnet mounted movably relative to the reactor 11, and at least means for moving the magnet relative to the reactor 11 between a position close to the reactor and a position far from the reactor 11. The magnet is, for example, a neodymium magnet. The chemical reaction module 10 also includes a mixer 13. Preferably, the mixer 13 is a static mixer. The mixer 13 thus includes one or more elements for generating turbulence in the flow passing through it. At least one of the elements forms a sinusoid. Advantageously, there is therefore no need to supply energy to the mixer 13 for it to operate, which limits the energy cost of such a phase.

[0071] The chemical reaction module 10 is also equipped with thermal control means. These thermal control means allow, in particular, for maintaining a zone 14 encompassing at least the reactor 11 at a given setpoint temperature. Preferably, said zone 14 also includes the magnetic trap 12 and / or the mixer 13.

[0072] The means of thermal regulation are, for example, controlled by the control system 7.

[0073] The thermal control means are configured here to heat zone 14. The thermal control means include, for example, one or more heating elements. The thermal control means are configured, for example, to heat said zone 14 to a temperature of 95 degrees Celsius and, for example, to heat said zone 14 to a temperature of 80 degrees Celsius.

[0074] Device 1 includes a main circuit 15 for circulating at least one fluid through the chemical reaction module 10.

[0075] The main circuit 15 thus includes a first connection system 16. The first connection system 16 is a three-way connection system, the first channel of which is connected to an outlet of reactor 11 and the second channel of which is connected to the inlet of mixer 13. The first connection system 16 thus allows the reactor 11 to be connected to mixer 13. Furthermore, the first connection system 16 is arranged outside zone 14.

[0076] The main circuit 15 also includes a second connection system 17. This connection system 17 is a three-way connection system, the third way of which is connected to the output of the mixer 13. Furthermore, the second connection system 17 is arranged outside the zone 14.

[0077] The main circuit 15 also includes at least one first pump 18 which, in operation, ensures the circulation of at least one fluid in the main circuit 15. The first path of the second connecting system 17 is connected to the inlet of the first pump 18. The outlet of the first pump 18 is itself connected to the inlet of the reactor 11. The first pump 18 is preferably a peristaltic pump. Furthermore, the second connecting system 17 is arranged outside the zone 14.

[0078] It is therefore understood that the main circuit 15 can operate in a closed loop. A fluid can thus circulate in the main circuit 15 successively through the first pump 18, the reactor 11, the first connecting system 16, the mixer 13, and the second connecting system 17, and again the first pump 18. However, the entire main circuit 15 is not included in zone 14. The inlet pipe is also connected to the main circuit 15. Preferably, the inlet pipe is connected to the main circuit 15 by at least one connecting system. For example, the inlet pipe is connected to the main circuit 15 by at least the second connecting system 17.

[0079] Preferably, the intake pipe is connected to the main circuit 15 via at least two connection systems.

[0080] For this purpose, device 1 includes a third connection system 19 which is connected to the second connection system 17. The third connection system 19 is, for example, a three-way connection system. Optionally, the first channel of the third connection system 19 is connected to the second channel of the second connection system 17. Furthermore, the inlet pipe is connected to the second channel of the third connection system 19.

[0081] Preferably, the device 1 includes at least one reservoir 20 containing at least one washing solution. The washing solution is, for example, a buffer solution, or even a phosphate saline buffer solution (or PBS solution). The washing solution reservoir 20 is, for example, connected to the third port of the third connection system 19.

[0082] Preferably, device 1 also includes an analysis module 21 connected to the chemical reaction module 10.

[0083] The analysis module 21 includes at least one sensor, such as an optical detection sensor. The optical detection sensor is, for example, a colorimeter 22. Preferably, the optical detection sensor is equipped with a modulated light source. In this way, the optical detection sensor exhibits good accuracy.

[0084] The analysis module 21 also includes at least one measuring tank associated with the optical detection sensor. In operation, a fluid flows through said tank, entering and exiting the analysis module 21. With the detection field of the optical detection sensor facing the tank, the optical detection sensor can estimate the presence of one or more components in the fluid, it being understood that the more intense the color of the result generated by the optical detection sensor, the greater the quantity of the component(s) present in said fluid.

[0085] Device 1 preferably includes a secondary circuit 23 linking the main circuit to the analysis module.

[0086] The secondary circuit 23 thus includes a fourth connection system 24. The fourth connection system 24 is, for example, a three-way connection system, the third way of which is here connected to an input of the colorimeter 22.

[0087] The secondary circuit 23 also includes at least one second pump 25 ensuring in service the circulation of at least one fluid in the secondary circulation circuit 23.

[0088] The third port of the first connection system 16 is connected here to the inlet of the second pump 25. The outlet of the second pump 25 is itself connected to the first port of the fourth connection system 24. The second pump 25 is preferably a peristaltic pump. The second pump 25 may be identical to the first pump 18 or may be different.

[0089] The secondary circuit 23 also includes at least one waste recovery tank 26. Preferably, the output of the colorimeter 22 is connected to the waste recovery tank 26 and / or the second channel of the fourth connection system 24 is also connected to the waste recovery tank 26.

[0090] It is therefore understood that the secondary circuit 23 operates in an open loop. The fluid can thus circulate in the secondary circuit 23 successively through the second pump 25, the fourth connection system 24 and the waste recovery tank 26 or successively through the second pump 25, the fourth connection system 24, the colorimeter 22 and the waste recovery tank 26.

[0091] Device 1 also includes a storage module 27 connected to the chemical reaction module 10 and / or the main circuit 15. The storage module 27 comprises several tanks, which are preferably independent. Each tank contains a product different from those contained in the other tanks.

[0092] Preferably, each tank is connected to the chemical reaction module 10 and preferably to the inlet of reactor 11 of said module. Preferably, each tank is connected to the chemical reaction module 10 independently of the other tanks. In the present case, each tank is connected directly to reactor 11.

[0093] A first reservoir 31 contains a first reagent, a second reservoir 32 contains a second reagent, a third reservoir 33 contains a third reagent and a fourth reservoir 34 contains a fourth reagent.

[0094] The first reagent is, for example, a hybridization buffer solution, such as a sodium citrate-based hybridization buffer solution containing a strand of DNA probe called a "signal probe." This signal probe is bound to a specific protein complementary to the DNA of a particular target protein (hereafter simply called the "target") for which we wish to determine certain information, such as its presence and / or concentration in the fluid being studied. The protein bound to the signal probe is, for example, horseradish peroxidase (also known as HRP). The hybridization buffer solution thus allows us to control the specificity of the hybridization reaction.

[0095] The second reagent is, for example, an antibody solution and, for example, an antibody solution recognizing the target bound to a given chromophore group, such as the chromophore group of the DNA-HRP complex. While the hybridization buffer solution is specific to the target being sought, the antibody solution is generic. The antibody solution thus makes the hybridization visible and detectable.

[0096] The third reagent is, for example, a detection solution, such as an oxidizing detection solution. The detection solution may be, or contain, 3,3',5,5' tetramethylbenzidine (better known by its acronym TMB). Such a solution will oxidize the chromophore group of the DNA-HRP complex to generate a colored oxidation product. Unlike the hybridization buffer, the detection solution is generic.

[0097] The fourth reagent is, for example, an acidic solution, which will finalize the oxidation of the chromophore group and stabilize the color of the oxidation product. Unlike the hybridization buffer solution, the acidic solution is generic.

[0098] These reagents are cited here only as examples, at least one of the reservoirs 31, 32, 33 and 34 being able to contain any other type of reagent.

[0099] Furthermore, the storage module 27 includes a fifth reservoir 35 containing beads suitable for cooperating with the magnetic trap 12. For this purpose, the beads are magnetic beads, for example, magnetic beads pre-functionalized with a DNA probe strand specific to and complementary to the target DNA. This DNA probe strand is preferably different from that of the hybridization buffer solution. Preferably, this probe strand contains DNA from a region adjacent to that of the DNA in the hybridization buffer solution.

[0100] During operation, when the magnet of the magnetic trap 12 is in the close position, the balls are magnetically attracted to the magnet and are thus pressed against one or more internal walls of the reactor 11 opposite the magnet (which remains external to the reactor 11). When the magnetic trap's magnet is in the far position, the balls are outside the magnet's field of attraction and can thus circulate freely within the reactor 11.

[0101] Preferably, at least one of the reservoirs is equipped with a metering pump, not referenced in the figures, in order to inject the product contained in said reservoir, dose by dose, into reactor 11. Preferably, each reservoir is equipped with a metering pump in order to inject the product contained in the reservoir concerned, dose by dose, into reactor 11. Optionally, at least one metering pump is a solenoid pump.

[0102] The use of dosing pumps makes it possible to introduce one or more reagents into reactor 11 in precise volumes.

[0103] Preferably, the storage module 27 is equipped with thermal control means. These thermal control means allow, in particular, the maintenance of a zone 30 encompassing at least one of the reactors of the storage module 27 at a given setpoint temperature. Preferably, said zone 30 includes all the tanks of the storage module 27, and preferably includes the entire storage module 27.

[0104] The means of thermal regulation are, for example, controlled by the control system 7.

[0105] The thermal control means are configured here to cool zone 30. These means include, for example, one or more Peltier cells and / or one or more fans. The thermal control means are configured, for example, to cool said zone 30 to a temperature below 10 degrees Celsius and preferably below 5 degrees Celsius. The thermal control means are configured, for example, to cool said zone 30 to a temperature of 4 degrees Celsius (plus or minus half a degree Celsius). By cooling zone 30, it is thus possible to improve the preservation of the various products present in the tanks.

[0106] Thus formed, the entire device 1 is contained within the box 2.

[0107] Device 1 therefore turns out to be relatively compact.

[0108] Furthermore, the device can be moved by a single person from point A to point B.

[0109] Device 1 proves to be particularly ergonomic.

[0110] Furthermore, device 1 contains all the elements necessary for its operation (power supply system 6, control system 7, chemical reaction module 10, ...). It is therefore completely autonomous.

[0111] Device 1 is also reusable.

[0112] Furthermore, device 1 allows for several detections to be carried out between two stays in a laboratory.

[0113] We will now describe how to proceed to detect at least one component present in a sample of a fluid using the device 1 which has just been described.

[0114] The first step of this process, illustrated in figure 3a, comprises three phases.

[0115] During the first phase of this first step, corresponding to the initial state of device 1, the device is configured as follows:

[0116] - the first distribution system 16 is switched off,

[0117] - the second distribution system 17 is switched off,

[0118] - the third distribution system 19 is switched off,

[0119] - The fourth distribution system 24 is switched off. Furthermore, the first pump 17 is powered so that it can operate in the first direction of rotation. The first pump 17 then operates in its "direct mode".

[0120] In this configuration, thanks to the first pump 17, the device 1 pumps a fluid from a natural or industrial environment to be studied, such as water from a watercourse (river, stream, ...) or from a body of water (lake, sea, swimming pool, body of water ...).

[0121] The fluid enters device 1 via the inlet pipe and reaches the third distribution system 19.

[0122] The fluid is then directed to the second distribution system 17 and thus enters the main circuit 15 via the second distribution system 17.

[0123] The fluid then flows from the second distribution system 17 into reactor 11.

[0124] After a predetermined time interval and / or a predetermined volume of fluid stored in reactor 11 (determined for example by means of a sensor linked to the second connection system 19, to the first pump 18 ...), the control system 7 controls device 1 to cease the implementation of the first phase and begin that of the second phase.

[0125] For this purpose, the control system 7 stops the first pump 18 and orders the supply of the dosing pump associated with the first reservoir 31.

[0126] The collection of a fluid sample is therefore done automatically by device 1 alone during this first phase.

[0127] During the second phase, the device is configured as follows: - the first distribution system 16 is switched off,

[0128] - the second distribution system 17 is switched off,

[0129] - the third distribution system 19 is switched off,

[0130] - the fourth distribution system 24 is switched off.

[0131] During the second phase, the dosing pump associated with the first reservoir 31 is thus supplied. Thanks to the dosing pump, a (optionally several) predetermined dose of the hybridization buffer solution is therefore injected into reactor 11.

[0132] After a predetermined time interval and / or a certain volume of hybridization buffer solution has been injected into the reactor, the control system 7 controls the device 1 to cease the implementation of the second stage and begin that of the third stage. To this end, the control system 7 stops the dosing pump connected to the first tank 31 and activates the first pump 18.

[0133] During the third phase, device 1 is configured as follows:

[0134] - the first distribution system 16 is switched off,

[0135] - the second distribution system 17 is switched on,

[0136] - the third distribution system 19 is switched off,

[0137] - the fourth distribution system 24 is switched off.

[0138] Furthermore, the first pump 18 is powered so that it can operate in its first direction of rotation. With this configuration, the fluid present in the reactor 11 circulates in the main circuit 15 in a closed loop: it is thus mixed at each passage through the mixer 13 of said main circuit 15.

[0139] Preferably, the control system 7 also activates the thermal regulation means of the chemical reaction module 10 to maintain zone 14 at a setpoint temperature above ambient temperature. Preferably, the setpoint temperature is between 50 and 80 degrees Celsius; and for example between 60 and 70 degrees Celsius; and for example between 64 and 66 degrees Celsius. In this way, the fluid that was present in reactor 11 at the end of the second phase circulates in the main circuit in a closed loop while being heated as it passes through zone 14.

[0140] The third phase is therefore similar to a heated incubation under agitation of the fluid which was present in reactor 11 at the end of the second phase, by the simple circulation of said fluid in the main circuit 15.

[0141] After a predetermined time interval and / or a given number of passes through the mixer 13, the control system 7 controls the device 1 to cease the implementation of the third phase and begin a second phase. To this end, the control system 7 stops the first pump 18 and the thermal control means, and activates the metering pump associated with the fifth tank 35. For example, the control system 7 controls the device 1 to cease the implementation of the third phase after a time interval of between 5 and 15 minutes, for example between 8 and 12 minutes, and for example between 9 and 11 minutes.

[0142] At the end of this third phase, reactor 11 is filled with a solution forming a first complex I. This first complex I is formed at least in part from the signal probe, which is now hybridized to its DNA strand complementary to the target present in the fluid under study (therefore resulting from the heated mixture of the sample and the hybridization buffer solution). The second step, illustrated in Figure 3b, then begins and comprises two phases.

[0143] During the first phase, the metering pump associated with the fifth reservoir 35 is thus supplied and the second connection system 17 is deactivated. Thanks to the metering pump, a (optionally several) predetermined dose of beads is therefore injected into the reactor 11.

[0144] After a predetermined time interval and / or a certain volume of beads has been injected into reactor 13, the control system 7 controls device 1 to cease the execution of the first phase and begin that of the second phase. To this end, the control system 7 stops the metering pump connected to the fifth tank 35 and activates the first pump 18.

[0145] During the second phase, the first pump 18 is powered to operate in its first direction of rotation, and the second connection system 17 is also activated. With this configuration, the fluid in the reactor circulates in the main circuit 15 in a closed loop: it is thus mixed at each passage through the mixer 13 of said main circuit 15.

[0146] Preferably, the control system 7 also activates the thermal regulation means of the chemical reaction module 10 to maintain zone 14 at a setpoint temperature above ambient temperature. Preferably, the setpoint temperature is between 50 and 80 degrees Celsius; and for example between 60 and 70 degrees Celsius; and for example between 64 and 66 degrees Celsius. In this way, the fluid that was present in reactor 11 at the end of the second phase circulates in the main circuit 15 in a closed loop while being heated as it passes through zone 14.

[0147] The second phase is therefore similar to a heated incubation under agitation of the fluid which was present in reactor 11 at the end of the first phase, by the simple circulation of said fluid in the main circuit 15.

[0148] After a predetermined time interval and / or a given number of passes through the mixer 13, the control system 7 controls the device 1 to cease the implementation of the second phase and begin a third step. For example, the control system 7 controls the device 1 to cease the implementation of the third phase after a time interval of between 15 and 25 minutes, for example between 18 and 22 minutes, and for example between 19 and 21 minutes.

[0149] At the end of this second phase, reactor 11 is filled with a solution forming a second complex II. This complex is made up at least in part of complex I captured by the specific probe linked to the magnetic beads, completing the sandwich hybridization (resulting from the mixing under heating of the first complex I with the magnetic beads).

[0150] The third step of this process, illustrated in figure 3c, comprises three phases.

[0151] During the first phase of this third step, the second connection system 17 is deactivated and the third connection system 19 is activated. The thermal control means are also deactivated. Furthermore, the first pump 18 remains powered and operates in its first direction of rotation.

[0152] In this configuration, thanks to the first pump 18, the device 1 pump from the reservoir 20 the washing solution.

[0153] The washing solution thus enters the main circuit 15 via the third distribution system 19 and the second distribution system 17. The washing solution then circulates from the second distribution system

[0154] 17 all the way into reactor 11.

[0155] After a predetermined time interval and / or a predetermined volume of fluid stored in reactor 11, the control system 7 controls device 1 to cease the implementation of the first phase and begin that of the second phase.

[0156] For this purpose, the control system 7 deactivates the third connection system 19 and activates the second connection system 17. Furthermore, the first pump

[0157] 18 is always powered and operates in its first direction of rotation.

[0158] With this configuration, the fluid present in reactor 11 circulates in the main circuit 15 in a closed loop: it is thus mixed at each passage through the mixer 13 of said main circuit 15.

[0159] Preferably, the control system 7 does not activate the thermal regulation means of the chemical reaction module 10.

[0160] The second phase is therefore similar to an incubation at room temperature of the fluid that was present in reactor 11 at the end of the first phase, by simply circulating said fluid in the main circuit 15. After a predetermined time interval and / or a given number of passes through the mixer, the control system 7 controls device 1 to cease the implementation of the second phase and begin a third phase. To this end, the control system 7 stops the first pump 18 and activates the second pump 25. For example, the control system 7 controls device 1 to cease the implementation of the second phase after a time interval of between 1 and 10 minutes, for example between 1 and 5 minutes, and for example between 2 and 3 minutes.

[0161] To begin the third phase, the control system 7 also deactivates the second connection system 17 and activates the first connection system 16. In this way, the main circuit 15 is in communication with the secondary circuit 23 and the main circuit 15 therefore no longer operates (temporarily) in closed loop.

[0162] Furthermore, the control system 7 also activates the magnetic trap 12. As a result, the magnet is brought into a close position, thus blocking the balls in the reactor 11.

[0163] The magnetic trap 12 therefore retains the balls in the reactor 11. On the other hand, the rest of the fluid present in the reactor 11 circulates from the reactor 11 to the first connection system 16 then to the second pump 25 then to the fourth connection system 24 and finally to the waste collector 26.

[0164] In this way, only the beads remain present in reactor 11, the rest of the solution being evacuated.

[0165] After a predetermined time interval and / or a predetermined volume of fluid has flowed out of reactor 11, the control system 7 controls device 1 to cease the implementation of the third phase. To this end, the control system 7 stops the second pump 25 and commands the metering pump associated with the second tank 32 to be supplied with fluid. Furthermore, the first connection system 16 and the magnetic trap 12 are deactivated. Care must be taken to stop the magnetic trap 12 once the first connection system 16 and / or the second pump 25 have stopped.

[0166] At the end of this third stage, reactor 11 is filled with the loaded beads. The beads are loaded with complex II. The beads are thus loaded with both target probes and capture probes.

[0167] According to a first option, the control system 7 controls the device 1 to then begin the implementation of a fourth step of the process.

[0168] According to a second option, the control system 7 controls the device 1 to start the implementation of one or more previous phases at least once more.

[0169] For example, the control system 7 controls the device 1 to successively repeat at least the following phases defining a complementary cycle:

[0170] - second phase of the first step (introduction of the hybridization buffer solution into reservoir 11);

[0171] - third phase of the first step and / or second phase of the second step (mixing and heating);

[0172] - the three phases of the third step (trapping the magnetic beads and removing the remaining solution). This additional cycle is, for example, carried out only once or several times, for example twice.

[0173] This improves the quality of the magnetic ball loading.

[0174] The fourth step of this process, illustrated in figure 3d, comprises two phases.

[0175] At the beginning of the first phase, the dosing pump associated with the second reservoir 32 is supplied. Thanks to the dosing pump, a (optionally several) predetermined dose of the antibody solution is thus injected into reactor 11.

[0176] After a predetermined time interval and / or a certain volume of antibody solution in reactor 11, the control system 7 controls device 1 to cease the execution of the first phase and begin the second phase. To this end, the control system 7 stops the dosing pump connected to the second reservoir 32.

[0177] During the second phase, neither the thermal regulation means of the chemical reaction module 10 nor the first pump 18 are activated.

[0178] The second phase is therefore similar to an incubation at room temperature and without agitation of the fluid that was present in reactor 11 at the end of the first phase.

[0179] After a predetermined time interval and / or a given number of passes through the mixer, the control system 7 controls device 1 to cease the execution of the second phase and begin a fifth step. For example, the control system 7 controls device 1 to cease the execution of the second phase after a time interval of between 10 and 20 minutes; and for example between 13 and 17 minutes; and for example between 14 and 16 minutes.

[0180] At the end of this fourth step, reactor 13 is filled with a solution forming a third complex III (resulting from the incubation of the loaded beads with the antibody solution).

[0181] The fifth step of this process, illustrated in figure 3e, comprises three phases.

[0182] During the first phase of this fifth step, the third connection system 19 is activated. Furthermore, the first pump 18 is powered and operates in its first direction of rotation.

[0183] In this configuration, thanks to the first pump 18, the device 1 pump from the reservoir 20 the washing solution.

[0184] The washing solution thus enters the main circuit 15 via the third distribution system 19 and the second distribution system 17. The washing solution then circulates from the second distribution system

[0185] 17 all the way into reactor 11.

[0186] After a predetermined time interval and / or a predetermined volume of fluid stored in reactor 11, the control system 7 controls device 1 to cease the implementation of the first phase and begin that of the second phase.

[0187] For this purpose, the control system 7 deactivates the third connection system 19 and activates the second connection system 17. Furthermore, the first pump

[0188] 18 is always powered and operates in its first direction of rotation. With this configuration, the fluid present in reactor 11 circulates in the main circuit 15 in a closed loop: it is thus mixed at each passage through the mixer 13 of said main circuit 15.

[0189] Preferably, the control system 7 does not activate the thermal regulation means of the chemical reaction module 10.

[0190] The second phase is therefore similar to an incubation at room temperature of the fluid that was present in reactor 11 at the end of the first phase, by the simple circulation of said fluid in the main circuit 25.

[0191] After a predetermined time interval and / or a given number of passes through the mixer 13, the control system 7 controls the device 1 to cease the execution of the second phase and begin a third phase. To this end, the control system 7 stops the first pump 18 and activates the second pump 25. For example, the control system 7 controls the device 1 to cease the execution of the second phase after a time interval of between 1 and 10 minutes, for example between 1 and 5 minutes, and for example between 2 and 3 minutes.

[0192] To begin the third phase, the control system 7 also deactivates the second connection system 17 and activates the first connection system 16. In this way, the main circuit 15 is in communication with the secondary circuit 23 and the main circuit 15 therefore no longer operates (temporarily) in closed loop.

[0193] Furthermore, the control system 7 also activates the magnetic trap 12. As a result, the magnet is brought into a close position, thus blocking the balls in the reactor 11.

[0194] The magnetic trap 12 therefore retains the balls in the reactor 11. On the other hand, the rest of the fluid present in the reactor 11 circulates from the reactor 11 to the first connection system 16 then to the second pump 25 then to the fourth connection system 24 and finally to the waste collector 26.

[0195] In this way, only the beads remain present in reactor 11, the rest of the solution being evacuated.

[0196] After a predetermined time interval and / or a predetermined volume of fluid has flowed out of reactor 11, the control system 7 controls device 1 to cease the implementation of the third phase. To this end, the control system 7 stops the second pump 25 and commands the metering pump associated with the third tank 33 to be supplied with fluid. Furthermore, the first connection system 16 and the magnetic trap 12 are deactivated. Care must be taken to stop the magnetic trap 12 once the first connection system 16 and / or the second pump 25 have stopped.

[0197] At the end of this fifth stage, reactor 11 is filled with the charged beads, complex III.

[0198] The marbles are thus charged with complex II.

[0199] According to a first option, the control system 7 controls the device 1 to then begin the implementation of a sixth step of the process.

[0200] According to a second option, the control system 7 controls the device 1 to restart the implementation of one or more previous phases at least once more. For example, the control system 7 controls the device 1 to successively restart at least the different phases of this fifth step, the three phases concerned thus defining a complementary cycle.

[0201] This additional cycle is for example carried out only once or is carried out several times and for example between 8 and 10 times and for example 9 times.

[0202] This improves the rinsing of the magnetic balls.

[0203] Next comes the sixth step, illustrated in figure 3f, which has two phases.

[0204] During the first phase, the dosing pump associated with the third reservoir 33 is thus supplied. Thanks to the dosing pump, a (optionally several) predetermined dose of a developing solution is therefore injected into reactor 11.

[0205] After a predetermined time interval and / or a certain volume of developing solution has been injected into reactor 11, the control system 7 controls device 1 to cease the execution of the first phase and begin that of the second phase. To this end, the control system 7 stops the dosing pump connected to the third reservoir 33 and activates the first pump 18.

[0206] During the second phase, the first pump 18 is powered to operate in its first direction of rotation, and the second connection system 17 is also activated. With this configuration, the fluid in the reactor 11 circulates in the main circuit 15 in a closed loop: it is thus mixed each time it passes through the mixer 13 of said main circuit 15. Preferably, the control system 7 does not activate the thermal regulation means of the chemical reaction module 10.

[0207] The second phase is therefore similar to an incubation at room temperature under agitation of the fluid which was present in reactor 11 at the end of the first phase, by the simple circulation of said fluid in the main circuit 15.

[0208] After a predetermined time interval and / or a given number of passes through the mixer 13, the control system 7 controls the device 1 to cease the implementation of the second phase and begin a seventh step. For example, the control system 7 controls the device 1 to cease the implementation of the second phase after a time interval of between 1 and 10 minutes; and for example between 3 and 7 minutes; and for example between 4 and 6 minutes.

[0209] At the end of this second phase, reactor 11 is filled with a solution colored by a redox reaction of the third complex III.

[0210] Next comes the seventh step, illustrated in figure 3g, which has two phases.

[0211] During the first phase, the dosing pump associated with the fourth tank 34 is supplied and the first pump 18 is deactivated. The second connection system 17 is also deactivated.

[0212] Using the metering pump, a predetermined dose (optionally several) of an acidic solution is injected into reactor 11. The acidic solution completes the oxidation of the chromophore group present in complex III. After a predetermined time interval and / or a certain volume of acidic solution has been injected into reactor 11, the control system 7 controls device 1 to stop the first phase and start the second phase. To this end, the control system 7 stops the metering pump connected to the fourth reservoir 34 and activates the second pump 25.

[0213] To begin the second phase, the control system 7 activates the first connection system 16 as well as the fourth connection system 24. In addition, the control system 7 activates the colorimeter 22.

[0214] In this way, the main circuit 15 is in communication with the secondary circuit 23 and the main circuit 15 therefore no longer operates (temporarily) in closed loop.

[0215] Furthermore, the control system 7 also activates the magnetic trap 12. As a result, the magnet is brought into a close position, thus blocking the balls in the reactor 11.

[0216] The magnetic trap 12 therefore retains the balls in the reactor 11. On the other hand, the rest of the fluid present in the reactor 11 circulates from the reactor 11 to the first connection system 16 then to the second pump 25 then to the fourth connection system 24 then to the colorimeter 22 and finally to the waste collector 26.

[0217] In this way, only the beads remain present in reactor 11, the rest of the solution being evacuated.

[0218] In this way, the fluid is discharged from reactor 11 via colorimeter 22. This allows for the detection of at least one component present in the sample taken. In particular, colorimetry will make it possible to determine the quantity of one or more components that were present in the fluid. At least one of these components is, for example, an indicator of fecal contamination and / or a specific pathogen.

[0219] Colorimetry is performed at a given wavelength or a given range of wavelengths. Colorimetry is performed, for example, at a wavelength between 350 and 550 nanometers; and, for example, at a wavelength between 400 and 500 nanometers; and, for example, at a wavelength of 450 nanometers (optionally to within 5 nanometers).

[0220] The result(s) obtained by the colorimeter 22 are for example recorded in a memory of the device 1 and / or transmitted outside the device 1 (for example by wifi).

[0221] After a predetermined time interval and / or a predetermined volume of fluid (passing through the colorimeter, exiting the reactor...), the control system 7 controls the device 1 to cease the implementation of the second phase and begin that of an eighth step.

[0222] At the end of this seventh step, reactor 11 is filled with the beads and an absorbance reading of the remaining fluid has been taken.

[0223] Next comes the eighth stage, illustrated in figure 3h, which comprises four phases.

[0224] During the first phase, the magnetic trap 12 is stopped. The marbles are then no longer blocked in the reactor 11. The colorimeter is also deactivated.

[0225] The beads then flow from reactor 11 to the first connection system 16 then into the second pump 25 then into the fourth connection system 24 then into the colorimeter 22 and finally into the waste collector 26.

[0226] After a predetermined time interval and / or a predetermined volume of fluid (passing through the colorimeter, exiting the reactor...), the control system 7 controls the device 1 to cease the implementation of the first phase and begin the second phase.

[0227] At the end of this first phase, reactor 11 is thus emptied.

[0228] To begin the second phase, the first connection system 16 is deactivated and the fourth connection system 24 is deactivated. The second pump 25 is also deactivated.

[0229] On the other hand, the third connection system 19 is activated as well as the first pump 18.

[0230] In this configuration, thanks to the first pump 18, the device 1 pump from the reservoir 20 the washing solution.

[0231] The buffer solution flows into the main circuit 25 via the third distribution system 19 and the second distribution system 17. The washing solution then flows from the second distribution system 17 into the reactor 11.

[0232] After a predetermined time interval and / or a predetermined volume of fluid stored in reactor 11, the control system 7 controls device 1 to cease the implementation of the second phase and begin that of the third phase.

[0233] For this purpose, the control system 7 deactivates the third connection system 19 and activates the second connection system 17. Furthermore, the first pump 18 is always supplied and operates in its first direction of rotation.

[0234] With this configuration, the fluid present in reactor 11 circulates in the main circuit 15 in a closed loop: it is thus mixed at each passage through the mixer 13 of said main circuit 15.

[0235] Preferably, the control system 7 does not activate the thermal regulation means of the chemical reaction module 10.

[0236] The second phase is therefore similar to a cleaning of the main circuit 15 as well as the chemical reaction module 10.

[0237] After a predetermined time interval and / or a given number of passes through the mixer, the control system 7 controls device 1 to cease the execution of the third phase and begin a fourth phase. To this end, the control system 7 stops the first pump 18 and activates the second pump 25. For example, the control system 7 controls device 1 to cease the execution of the second phase after a time interval of between 1 and 10 minutes, for example between 1 and 5 minutes, and for example between 2 and 3 minutes.

[0238] To begin the fourth phase, the control system 7 also deactivates the second connection system 17 and activates the first connection system 16 and the fourth connection system 24. In this way, the main circuit 15 is connected to the secondary circuit 23, and the main circuit 15 is therefore no longer (temporarily) operating in a closed loop. All the fluid present in the reactor 11 and the main circuit 15 flows from the reactor 11 to the first connection system 16, then to the second pump 25, then to the fourth connection system 24, then to the colorimeter 22, and finally to the waste collector 26.

[0239] In this way, the secondary circuit 23 and the analysis module 21 are also cleaned.

[0240] After a predetermined time interval and / or a predetermined volume of fluid has flowed out of reactor 11, the control system 7 controls device 1 to cease the implementation of the fourth stage. To this end, the control system 7 stops the second pump 25, the first connecting system 16 and the fourth connecting system 24.

[0241] Device 1 has then returned to its initial state and is ready to be reused once again.

[0242] It is understood that device 1, as described, allows for detection as well as automatic cleaning.

[0243] The operator's intervention on device 1 proves to be quick and simple.

[0244] Furthermore, it is not necessary to move device 1 between two successive detections.

[0245] Advantageously, even an untrained operator can easily perform the detection.

[0246] The detection by device 1 is also customizable. For example, the operator can specify the volume of acid solution, buffer solution, etc., that reactor 11 must contain, the sample volume, etc. These parameters can, for example, be programmed remotely via the communication interface 8 and / or the human-machine interface 9. Device 1 is also robust.

[0247] Device 1 is also compact.

[0248] Device 1 is relatively inexpensive to build and operate.

[0249] Advantageously, the various products used for the detection of at least one component present in the fluid are recovered and not discarded into the natural environment.

[0250] Furthermore, detection is relatively fast (on the order of a few hours).

[0251] Advantageously, device 1 is adaptable to different types of contamination indicators (fecal or pathogenic) to be studied in the sample, depending on the reagent(s) present in the reservoirs (for example, by adapting the nature of the antibody solution and / or the detection solution according to the indicator targeted). At least one contamination indicator can thus be a nucleic acid.

[0252] Device 1 as described thus plays the role of an automated molecular biosensor, for flow detection, for the measurement of at least one indicator of contamination in a fluid of a given environment.

[0253] Device 1 as described allows for the implementation of a first pre-analytical stage including phases of sampling, concentration and cell lysis as well as a second stage of analysis by recognition of a molecular target indicator (by sandwich hybridization coupled with an immunological assay).

[0254] Of course, the invention is not limited to the embodiment described but encompasses any variant falling within the scope of the invention as defined by the claims.

[0255] Although here the first and second pumps are peristaltic pumps, at least one of these pumps could be different and be, for example, a vane pump, a pneumatic diaphragm pump, etc.

[0256] The device may include other elements than what has been indicated, such as one or more filters associated with at least one of the pumps to protect it, etc.

[0257] The device may include a different number of connection systems than indicated or different connection systems.

[0258] Although it has been indicated here that the device can be installed in a fixed position in a given natural or industrial environment, the device can of course be installed on an object or a mobile vehicle in said natural and / or industrial environment (instrumented buoys, autonomous underwater robot, remotely operated underwater vehicle ...).

[0259] The device will be able to indicate the status of at least one parameter to the operator (via its communication interface and / or its human-machine interface) such as one or more flow rate data points from at least one of the pumps, one or more data points on the battery charge status...

[0260] Although the enclosure is configured here to withstand splashes and / or humid and saline environments, it can be configured to be fully submerged. Preferably, the enclosure will thus be watertight.

[0261] Although the device here operates on battery power, it can also operate on mains power. As a replacement for, or in addition to, mains battery charging, the battery can be recharged autonomously via one or more solar panels mounted on the housing (or any other renewable energy converter besides solar panels).

[0262] Although the device shown here has only one washing solution reservoir, it can have a different number of washing solution reservoirs. The connection system can therefore be configured to allow the user to choose which washing solution reservoir will be connected to the main circuit. This allows the main circuit to be rinsed with one or more washing solutions (for example, depending on the reagents introduced into the reactor). The eighth washing step can also be performed multiple times instead of just once, as described above.

[0263] The number of tanks present in the storage module may be greater or less than indicated.

[0264] The device may include one or more sensors other than those indicated. For example, the device may include at least one sensor communicating with the control system to manage device 1. This sensor may be a pressure, flow rate, temperature, salinity, turbidity, etc. sensor. For example, the sensor may be a flow rate sensor. For example, the sensor may be a flow rate sensor measuring the flow rate of the fluid passing through the feed pipe and / or exiting the reactor.

[0265] Although here the thermal regulation means of the chemical reaction module only work when it is desired to heat the associated zone, the thermal regulation means can work continuously to maintain said zone at a given setpoint temperature.

[0266] It will be possible to do without temperature regulation means for one, several or all of the storage tanks.

[0267] Although here the optical sensor allows the detection of at least one component in a fluid at a given wavelength, it can also be used to perform such detection for at least two different wavelengths (or at least two different wavelength ranges). For example, the optical sensor can be controlled to take a measurement at a first wavelength and (simultaneously or alternatively) to take a measurement at a second wavelength. One of the wavelengths could be 450 nanometers and the other 630 nanometers. For example, it would be possible to take a measurement at 630 nanometers and then a measurement at 450 nanometers.

[0268] At least one measurement may be carried out at a different wavelength (or range of wavelengths) than indicated. For example, at least one measurement may be carried out at a wavelength between 550 and 700 nanometers, and for example at a wavelength between 600 and 650 nanometers, and for example at a wavelength of 630 nanometers (optionally to within ±5 nanometers).

[0269] The analysis module may include several measuring cuvettes associated with the optical sensor. This will allow measurements to be taken in parallel and / or at different wavelengths.

[0270] The analysis module can be integrated into the chemical reaction module, for example by being integrated into the reactor.

[0271] Although here the detection sensor is a colorimeter, the sensor could be different and be, for example, a fluorescence measurement sensor or an electrochemical sensor.

[0272] Although here there is no buffer zone between the refrigerated tanks and the heated zone of the chemical reaction module, the system may include one or more buffer zones between at least one refrigerated tank and the heated zone.

[0273] Although the same marbles are used throughout the various steps mentioned above, different marbles can be used between the different steps mentioned above.

[0274] Although the different tanks here are at the same setpoint temperature, the different tanks can be at different setpoint temperatures. The system can therefore have several thermal control methods associated with different tanks. These thermal control methods can be coupled with those of the reactor.

[0275] Although here only one zone of the main circuit is in the part regulated in temperature by the regulating means, the entire main circuit can be arranged in this zone.

[0276] Although here, during the first phase of the eighth step, the marbles are discharged by passing through the colorimeter, they can also be discharged directly without passing through the colorimeter. Other steps than those indicated may be added, and / or other phases and cycles than those indicated. In particular, one or more additional cycles may be added between two steps beyond what has been indicated. The container may take a different form than the one indicated. For example, the container may be mounted on casters and / or have a telescopic handle. The container may thus be shaped into a briefcase.

Claims

DEMANDS 1. A self-contained device (1) for detecting at least one component present in a fluid, comprising a housing (2) in which at least: - a chemical reaction module (10) equipped with a reactor (11), a magnetic trap (12), and means for thermal regulation of the chemical reaction module, - a main circuit (15) for circulating at least one fluid through the reactor and the magnetic trap, - at least one main pump (18) ensuring, in operation, the circulation of at least one fluid in the main circulation circuit, - at least one reservoir (35) of magnetic beads associated with a first metering pump for injecting doses of magnetic beads into the main circuit, - at least one reservoir of at least one reagent associated with a second metering pump for injecting the reagent into the main circuit in doses, - a control system (7) of at least the main pump, the first metering pump, the second metering pump, the thermal control means and the magnetic trap.

2. Device according to claim 1, wherein the main pump (18) is a peristaltic pump.

3. Device according to any one of claims 1 to 2, wherein at least one of the metering pumps is a solenoid pump.

4. A device according to any one of claims 1 to 3, wherein the chemical reaction module (10) comprises in addition at least one static mixer (13), the main circuit being arranged to allow the fluid to circulate in a loop between the reactor (11) and the mixer.

5. Device according to any one of the preceding claims, comprising means for maintaining the reagent reservoir at a set temperature.

6. Device according to claim 5, wherein the retaining means comprise a Peltier effect cell.

7. Device according to any one of the preceding claims, comprising a secondary circuit (23) connected to the main circuit, the secondary circuit also extending into the casing.

8. Device according to claim 7, wherein the secondary circuit (23) comprises a second pump.

9. Device according to any one of claims 7 to 8, wherein the secondary circuit comprises at least one analysis module (21).

10. Device according to claim 9, wherein the analysis module comprises a colorimeter (22).

11. A method for controlling a fluid using a device according to any one of the preceding claims, comprising the steps of: - Introduce at least one fluid into the main circuit (15), - Inject at least one dose of at least one reagent into the main circuit, - Inject at least one dose of magnetic beads into the main circuit, - Heat the mixture of fluid, beads and reagent in reactor (11), - Trap the magnetic beads in the magnetic trap in order to analyze the components trapped on the beads.

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