Biological tissue transfer device and method

Abstract

Disclosed in the present application are a biological tissue transfer device and method. The device comprises: a carrier rod, which contains a liquid comprising at least biological tissue; and a fixture, on which the carrier rod is clamped, one end of the carrier rod being in contact with one end of a cannula clamped on the fixture, wherein the carrier rod and / or the cannula move axially relative to each other, such that the biological tissue in the carrier rod is transferred into the cannula. The present application is simple to operate and easy to implement, avoids damage to the transferred embryos, and prevents the loss of embryos during the transfer process.

Description

Biological tissue transfer device and method thereof TECHNICAL FIELD

[0001] The present application relates to the field of bioengineering technology, and in particular to a biological tissue transfer device and method thereof. BACKGROUND

[0002] Although the freezing technology, especially the vitrification freezing technology, has achieved remarkable success in the field of human medicine and is widely used in reproductive medicine, its application in the livestock industry has been greatly limited. This limitation is mainly affected by factors such as cost, professional knowledge, and pasture environment. First, vitrification freezing requires expensive equipment and professional knowledge, which may be impractical for the livestock industry. Specifically, the embryos after thawing need to be transferred to the recipient cow as soon as possible, and the embryo technician needs to use a mouth pipette to transfer the embryos in the carrier to the straw under the help of a microscope, which requires meticulous training and a professional laboratory environment. In the pasture environment, there is usually a lack of personnel with these professional skills, so it is difficult to apply this technology. In addition, the pasture is usually located in remote areas, lacking the necessary infrastructure and equipment such as microscopes and freezing equipment, making the application of the technology more difficult.

[0003] In the prior art, embryo transfer requires skilled embryo technicians to operate, and the thawed embryos need to be transferred to the recipient cow as soon as possible, which will lead to a decrease in the success rate of operation. In addition, the manual embryo transfer and straw filling operations are complicated, inefficient, and prone to errors. SUMMARY

[0004] The main purpose of the present application is to provide a biological tissue transfer device and method thereof to solve the problems of complex operation and low success rate in the existing embryo transfer technology in the livestock industry.

[0005] According to one aspect of the present application, a biological tissue transfer device is provided, which comprises: a carrier containing a liquid comprising at least biological tissue; a clamp, the carrier being clamped on the clamp, one end of the carrier being in contact with one end of a sleeve clamped on the clamp; wherein the carrier and / or the sleeve move relatively to each other to transfer the biological tissue in the carrier to the sleeve.

[0006] The carrier and / or the sleeve move relatively to each other in an axial direction.

[0007] The axial movement is an axial reciprocating movement.

[0008] The axial reciprocating movement is one or more acceleration movements.

[0009] Acceleration is generated by electromagnetic force.

[0010] The clamp drives the carrier rod to perform axial reciprocating movement to transfer the biological tissue in the carrier rod into the sleeve.

[0011] The clamp comprises a clamp body, a first clamping part clamping the carrier rod, and a second clamping part clamping the sleeve, the first clamping part is movably connected with the clamp body, and the first clamping part clamps the carrier rod to move axially relative to the clamp body.

[0012] The clamp further comprises a slider arranged below the first clamping part, and a sensor detecting the axial movement of the first clamping part driven by the slider.

[0013] The biological tissue transfer device further comprises a docking part arranged between the first clamping part and the second clamping part, the docking part comprises a horn-shaped part defining the docking position of the carrier rod and the sleeve.

[0014] The docking part comprises a positioning part, at least one carrier rod positioning hole is arranged on one side of the positioning part, at least one sleeve positioning hole is arranged on the other side of the positioning part and communicates with the at least one carrier rod positioning hole, and the at least one carrier rod positioning hole and the at least one sleeve positioning hole gradually expand from inside to outside in a horn shape.

[0015] The biological tissue transfer device further comprises one or more carrier rod supporting frames, the carrier rod supporting frames are arranged on the clamp, and each carrier rod supporting frame is used for carrying a plurality of carrier rods.

[0016] The number of the carrier rods is 1-3.

[0017] The carrier rod comprises a handle, a carrier rod body, and a front end sheet, the front end sheet has a groove, and the groove contains at least a liquid containing biological tissue.

[0018] The biological tissue transfer device further comprises an automatic filling device for sucking external liquid into the sleeve, and an electric control device for controlling the axial movement of the carrier rod or the sleeve.

[0019] The biological tissue transfer device further comprises a reset device for resetting the carrier rod or the sleeve.

[0020] The sleeve is a straw.

[0021] The biological tissue comprises an embryo or an ovum.

[0022] According to another aspect of the present application, a method for transferring biological tissue is provided, which comprises: clamping a carrier rod and a sleeve on a clamp, respectively, wherein the carrier rod contains a liquid including biological tissue; and contacting one end of the carrier rod containing the biological tissue with one end of the sleeve, wherein the carrier rod and / or the sleeve are relatively moved to transfer the biological tissue in the carrier rod to the sleeve.

[0023] wherein the carrier rod and / or the sleeve are relatively axially moved.

[0024] wherein the axial movement is an axial reciprocating movement.

[0025] wherein the axial reciprocating movement is one or more acceleration movements.

[0026] wherein the acceleration is generated by an electromagnetic force.

[0027] wherein the method further comprises: the clamp driving the carrier rod to axially reciprocate to transfer the biological tissue in the carrier rod to the sleeve.

[0028] wherein the clamp comprises: a clamp body, a first clamping part and a second clamping part, the first clamping part being movably connected with the clamp body; and the method further comprises: clamping the carrier rod on the first clamping part and clamping the sleeve on the second clamping part, so that the first clamping part clamps the carrier rod to axially move relative to the clamp body.

[0029] wherein the clamp further comprises a slider disposed below the first clamping part, and the first clamping part has a locking structure for locking the carrier rod; and the method further comprises: the slider sliding on the clamp body, and the slider driving the first clamping part and the carrier rod clamped thereby to axially reciprocate.

[0030] wherein the method further comprises: providing a docking part between the first clamping part and the second clamping part of the clamp, and the docking part comprises a horn-shaped part defining a docking position of the carrier rod and the sleeve.

[0031] wherein the docking part comprises a positioning part, one side of the positioning part is provided with at least one carrier rod positioning hole, and the other side of the positioning part is provided with at least one sleeve positioning hole in communication with the at least one carrier rod positioning hole, and the at least one carrier rod positioning hole and the at least one sleeve positioning hole gradually expand from inside to outside in a horn shape; and the carrier rod positioned by the carrier rod positioning hole and the sleeve positioned by the sleeve positioning hole are in contact at the docking part.

[0032] The method further comprises: arranging one or more carrier rods on the clamp, wherein each carrier rod is used to carry a plurality of carrier rods.

[0033] The number of the carrier rods is 1-3.

[0034] The carrier rod comprises: a handle, a carrier rod body, and a front end sheet, wherein the front end sheet is provided with a groove, and the groove is used to hold a liquid containing biological tissue.

[0035] The method further comprises: using an automatic filling device to suck the liquid outside into the sleeve, and transfer the biological tissue from the carrier rod to the liquid in the sleeve; and using an electric control device to control the axial movement of the carrier rod or the sleeve, so that one end of the carrier rod containing the biological tissue is in contact with one end of the sleeve.

[0036] The method further comprises: using a reset device to reset the carrier rod or the sleeve.

[0037] The sleeve is a straw.

[0038] The biological tissue comprises an embryo or an ovum.

[0039] According to the technical solution of the present application, the acceleration of the carrier rod and / or the sleeve is used to realize the axial movement, so that the embryo in the carrier rod can be directly transferred to the sleeve (without the need of a mouth suction tube). The above transfer process does not need the help of a microscope, and is convenient to use in a pasture or a remote environment. In addition, according to the technical solution of the present application, the operation is simple and easy to implement, the transferred embryo is not damaged, and the embryo is also prevented from being lost during the transfer process. BRIEF DESCRIPTION OF DRAWINGS

[0040] Fig. 1 is a schematic view of a biological tissue transfer device according to an embodiment of the present application;

[0041] Fig. 2 is a schematic view of a clamp according to an embodiment of the present application;

[0042] Fig. 3 is a schematic view of a first clamping part according to an embodiment of the present application;

[0043] Fig. 4 is a schematic view of a carrier rod according to an embodiment of the present application;

[0044] Fig. 5 is a schematic view of a first clamping part and a power device according to an embodiment of the present application;

[0045] Figs. 6A and 6B are schematic views of a docking part according to an embodiment of the present application;

[0046] Fig. 7 is a schematic view of a biological tissue transfer device according to another embodiment of the present application;

[0047] Fig. 8 is a schematic view of a carrier bar support frame according to an embodiment of the present application;

[0048] Fig. 9 is a schematic view of a carrier bar support frame according to another embodiment of the present application. DETAILED DESCRIPTION

[0049] For the purpose of making the objects, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described below in detail with reference to the embodiments of the present application and corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

[0050] The technical solutions provided by the embodiments of the present application will be described in detail below with reference to the drawings.

[0051] With reference to Figs. 1 and 2, the biological tissue transfer device according to the embodiments of the present application comprises a clamp 10 and a carrier bar 20. The clamp 10 comprises a clamp body 11, a first clamping portion 12 and a second clamping portion 13. The first clamping portion 12 is arranged near one end of the clamp body 10, and is used to clamp and fix the carrier bar 20, and can drive the carrier bar 20 to move when the first clamping portion 12 moves.

[0052] With reference to Figs. 2 and 3, the first clamping portion 12 is installed at one end of the clamp body 11, and is used to clamp the carrier bar 20 and can drive the carrier bar 20 to move axially or radially relative to the clamp body 11. Specifically, the first clamping portion 12 is defined with one or more clamping grooves 121, and the carrier bar 20 can be clamped in the clamping grooves 121 and locked in the clamping grooves 121 by a locking structure 122, wherein the locking structure 122 can be a pin shaft. With continued reference to the drawings, a sliding block 125 fixedly connected with the first clamping portion 12 is further arranged on the clamp body 11, and the sliding block 125 can slide axially on the clamp body 11, so that the sliding block 125 can drive the first clamping portion 12 and the carrier bar 20 clamped thereby to reciprocate axially on the clamp body 11.

[0053] With reference to Fig. 4, the carrier bar 20 is generally in a long strip shape, and comprises a handle 21, a carrier bar body 22 and a front end sheet 23. The handle 21 is a position directly contacted and operated by an operator's hand. The front end sheet 23 is provided with at least one recess 231, for example, two or more recesses can be provided. The recess 231 is used to hold biological materials such as embryos, ova or cells and related solutions for protecting the biological materials. When the carrier bar 20 is fixed, the position close to the handle 21 is clamped in the clamping groove 121.

[0054] At the other end of the first clamping portion 12, a second clamping portion 13 is provided for clamping and fixing the sleeve 30. The second clamping portion 13 comprises a plurality of clamping grooves, the height of the clamping grooves of the second clamping portion is the same as the height of the clamping grooves of the first clamping portion 12, so that the sleeve 30 and the carrier rod 20 are in the same horizontal position, so as to facilitate the butt joint or contact of the sleeve 30 and the carrier rod 20. The volume of the sleeve 30 is 0.25-0.5 mL, which can be one of a straw, a centrifuge tube, and a test tube, is made of biocompatible material, and can be used for cryopreservation of oocyte and embryo samples, or for transferring embryos or sperm to target animals. In the embodiment, the second clamping portion 13 is fixedly connected with the clamp body 11, or the second clamping portion 13 is integrally formed with the clamp body 11, when the carrier rod 20 (clamped by the first clamping portion 12) moves axially relative to the clamp body 11, the sleeve 30 (clamped by the second clamping portion 13) is fixed relative to the clamp body 11.

[0055] In some other embodiments, the carrier rod 20 (clamped by the first clamping portion 12) is fixed relative to the clamp body 11, while the sleeve 30 (clamped by the second clamping portion 13) moves axially relative to the clamp body 11.

[0056] Or in some other embodiments, the carrier rod 20 (clamped by the first clamping portion 12) moves axially relative to the clamp body 11, and the sleeve 30 (clamped by the second clamping portion 13) also moves axially relative to the clamp body 11. It should be noted that the above-mentioned axial movement can be reciprocating movement, shaking, shaking or movement, and in the above-mentioned movement, the carrier rod 20 and the sleeve 30 always remain in contact.

[0057] In the above movement, the acceleration can be generated by electromagnetic force, mechanical force, friction force, etc. to make the biological tissue such as embryo carried in the groove of the carrier rod 20 fall into the sleeve 30. Hereinafter, the mode that the carrier rod 20 is driven to move axially by electromagnetic force and the sleeve 30 is fixed is taken as an example for detailed description. Referring to FIG. 5, the driving device 70 is connected with the sliding block 125 through a shaft, the driving device 70 is used to generate the action of pulling the sliding block 125 backward (the direction from the clamping part 12 to the device 70), and in practice, the driving device 70 can be an electromagnet. Another reset device 75 is connected with the device 70, and the reset device 75 is used to generate the action of resetting, and in practice, the reset device 75 can be an electromagnet or a screw motor. In this way, the cooperation of the driving device 70 and the reset device 75 can make the sliding block 125 move axially and reciprocally, thereby driving the carrier rod 20 clamped thereby to move axially and reciprocally. The number of reciprocations can be 1 to 100, or 1-10. The stroke of the front end of the carrier rod can be 0.01mm-30mm, or 0.01mm-5.5mm. Further, in some embodiments of the present application, a sensor (not shown) for detecting the axial movement of the sliding block 125 relative to the clamping body 11 can be further included, and the sensor can be used to determine whether the sliding block 125 has completed the axial movement.

[0058] It should be noted that in the above embodiments, the driving device 70 and the reset device 75 are separately arranged. However, in other embodiments, the driving device 70 and the reset device 75 can be arranged together, which will not be described herein.

[0059] Referring to FIGS. 6A and 6B, in order to facilitate the butt joint of the carrier rod 20 and the sleeve 30 and make them accurately aligned, a butt joint part 15 is further arranged between the first clamping part 12 and the second clamping part 13, the butt joint part 15 includes a trumpet shape for defining the butt joint position of the carrier rod 20 and the sleeve 30, the carrier rod 20 and the sleeve 30 are close to the butt joint part 15 from both sides of the butt joint part 15, the trumpet of the butt joint part 15 is open at both ends and expands outward from inside, and it receives and guides the carrier rod 20 and the sleeve 30 to facilitate the alignment of the end portions of the carrier rod 20 and the sleeve 30. Specifically, the butt joint part 15 includes a positioning part 16, at least one carrier rod positioning hole 161 and at least one sleeve positioning hole 162 are respectively arranged at both sides of the positioning part 16. The carrier rod positioning hole 161 and the sleeve positioning hole 162 are communicated, that is, the carrier rod positioning hole and the sleeve positioning hole are one-to-one corresponding and communicated. The carrier rod positioning hole 161 and the sleeve positioning hole 162 are trumpet-shaped and gradually expand outward from inside, the carrier rod 20 positioned by the carrier rod positioning hole 161 contacts the sleeve 30 positioned by the sleeve positioning hole 162 at the butt joint part 15.

[0060] In the above embodiment, three carriers are shown, but the number of carriers is not limited by the present application. In the case of multiple carriers, the carriers can be supported by a carrier support frame. Referring to Figs. 7 and 8, the carrier support frame 50 includes a groove for supporting multiple carriers. The carrier support frame 50 is arranged on the clamp 10, which can be mounted between the first clamping portion 12 and the abutting portion 15 or between the first clamping portion 12 and the second clamping portion 13. By providing the carrier support frame, one or more carriers can be simultaneously supported, the multiple carriers can be more conveniently moved together, and the direction of the carriers can be fixed, thereby improving work efficiency. It should be noted that the biological tissue transfer device according to the embodiment of the present application can include one or more carrier support frames 50, and each carrier support frame 50 can be used to support one or more carriers, for example, 1-3 carriers.

[0061] Referring to Fig. 9, according to the embodiment of the present application, one end of the sleeve 30 is in contact with the carrier 20 to receive the embryo detached from the carrier 20. The other end of the sleeve 30 is connected to an automatic filling and electric control device 80, which has an automatic filling function for sucking liquid from the outside and injecting the liquid into the sleeve 30 to form at least one section of liquid in the sleeve 30. The liquid can be one of a culture solution, a transplantation solution, or an operation solution. The device 80 can be a manual negative pressure device or an automatic negative pressure device. The manual negative pressure device is one of a syringe, a rubber-tipped pipette, or a mouth pipette. The automatic negative pressure device is one of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, a vane pump, or a piston pump.

[0062] The device 80 can have a liquid inlet and a liquid outlet. The liquid inlet is connected to an external test tube through a connecting pipe, and the liquid outlet is connected to each sleeve 30 through a connecting pipe. The automatic negative pressure device sucks the liquid in the external test tube by negative pressure and injects the liquid into the sleeve 30.

[0063] A heating device 85 is further arranged on the upper surface of the device 80, which is used to heat the solution required for embryo transplantation and / or thawing oil. In addition, the device 80 has a control function, which can be used to control the axial movement of the carrier or the sleeve. Specifically, the device 80 can be electrically connected to the driving device 70, so as to drive the carrier 20 to perform axial reciprocating movement by controlling the driving device 70, and to drive the sleeve 30 to perform axial reciprocating movement by controlling another driving device (not shown).

[0064] According to the embodiment of the present application, a biological tissue transfer method is further provided, which includes:

[0065] The carrier and the sleeve are clamped on the clamp, respectively. The carrier contains a liquid including at least biological tissue. The sleeve is a straw, and the biological tissue includes an embryo or an ovum.

[0066] contacting one end of the carrier rod containing the biological tissue with one end of the sleeve, wherein the carrier rod and / or the sleeve are relatively axially moved to transfer the biological tissue in the carrier rod into the sleeve.

[0067] wherein the axial movement is an axial reciprocating movement, which can be one or more acceleration movements, such as 1 to 100 or 1 to 10 acceleration movements, and the acceleration is generated by electromagnetic force, and the carrier rod or the sleeve is reset by a reset device.

[0068] wherein the clamp drives the carrier rod to perform axial reciprocating movement to transfer the biological tissue in the carrier rod into the sleeve.

[0069] wherein the clamp comprises a clamp body, a first clamping part and a second clamping part, the first clamping part is movably connected with the clamp body; the method further comprises clamping the carrier rod on the first clamping part and clamping the sleeve on the second clamping part, so that the first clamping part clamps the carrier rod to move axially relative to the clamp body.

[0070] wherein the clamp further has a sliding block fixedly connected with the first clamping part, and the first clamping part has a locking structure for locking the carrier rod; the method further comprises that the sliding block slides axially on the clamp body, and the sliding block drives the first clamping part and the carrier rod clamped thereby to perform axial reciprocating movement.

[0071] Further, a docking part is arranged at a position between the first clamping part and the second clamping part of the clamp, and the docking part comprises a horn-shaped shape defining a docking position of the carrier rod and the sleeve. Specifically, the docking part comprises a positioning part, one side of the positioning part is provided with at least one carrier rod positioning hole, the other side of the positioning part is provided with at least one sleeve positioning hole in communication with the at least one carrier rod positioning hole, and the at least one carrier rod positioning hole and the at least one sleeve positioning hole gradually expand from inside to outside in a horn shape; wherein the carrier rod positioned by the carrier rod positioning hole contacts the sleeve positioned by the sleeve positioning hole at the docking part.

[0072] In addition, one or more carrier rod support frames are arranged on the clamp, and each of the carrier rods is used to carry a plurality of carrier rods. For example, one, two or three carrier rod support frames are arranged on the clamp, and each carrier rod can be used to carry three carrier rods.

[0073] wherein the carrier rod comprises a handle, a carrier rod body and a front end sheet, the front end sheet has a groove, and at least a liquid containing biological tissue is contained in the groove.

[0074] Further, an external liquid is drawn into the sleeve by an automated filling device, and the biological tissue from the carrier rod is transferred into the liquid in the sleeve; and the carrier rod or the sleeve is controlled to move axially by an electric control device, so that one end of the carrier rod containing the biological tissue is in contact with one end of the sleeve.

[0075] While the disclosure has been described in detail with respect to specific embodiments thereof, it will now be apparent to those skilled in the art that various alterations and modifications can be made therein without departing from the spirit and scope of the embodiments. Therefore, it is the intention that the application not be limited to the described embodiments, but should be limited only by the scope of the appended claims and their equivalents.

Claims

1. A biological tissue transfer device, characterized by, The application relates to a device for transferring biological tissue, comprising: a carrier rod containing liquid including biological tissue; a clamp, the carrier rod being clamped on the clamp, one end of the carrier rod being in contact with one end of a sleeve clamped on the clamp; wherein the carrier rod and / or the sleeve move relatively to each other to transfer the biological tissue in the carrier rod to the sleeve.

2. The biological tissue transfer device of claim 1, wherein, The carrier rod and / or the sleeve move relatively to each other in an axial direction.

3. The biological tissue transfer device of claim 1, wherein, The axial movement is an axial reciprocating movement.

4. The biological tissue transfer device of claim 3, wherein, The axial reciprocating movement is one or more acceleration movements.

5. The biological tissue transfer device of claim 4, wherein, Acceleration is generated by electromagnetic force.

6. The biological tissue transfer device of claim 1, wherein, The clamp drives the carrier rod to move in an axial reciprocating movement to transfer the biological tissue in the carrier rod to the sleeve.

7. The biological tissue transfer device of claim 1, wherein, The clamp comprises a clamp body, a first clamping part clamping the carrier rod and a second clamping part clamping the sleeve, the first clamping part being movably connected to the clamp body, and the first clamping part clamping the carrier rod to move axially relative to the clamp body.

8. The biological tissue transfer device of claim 7, wherein, The clamp further comprises a sliding block arranged below the first clamping part and a sensor detecting the axial movement of the first clamping part driven by the sliding block.

9. The biological tissue transfer device of claim 7, wherein, The application further relates to a device for transferring biological tissue, comprising: a butt joint part arranged between the first clamping part and the second clamping part, the butt joint part comprising a horn shape defining a butt joint position of the carrier rod and the sleeve.

10. The biological tissue transfer device of claim 9, wherein, The butt joint part comprises a positioning part, one side of the positioning part being provided with at least one carrier rod positioning hole, and the other side of the positioning part being provided with at least one sleeve positioning hole in communication with the at least one carrier rod positioning hole, the at least one carrier rod positioning hole and the at least one sleeve positioning hole gradually expanding from inside to outside in a horn shape.

11. The biological tissue transfer device of claim 1 or 9, wherein, The application further relates to a device for transferring biological tissue, comprising: one or more carrier rod supporting frames arranged on the clamp, each of the carrier rod supporting frames being used for supporting one or more carrier rods.

12. The biological tissue transfer device of claim 11, wherein, The number of carrier rods supported by the carrier rod supporting frame is 1-3.

13. The biological tissue transfer device of claim 1, wherein, The carrier rod comprises a handle, a carrier rod body and a front end sheet, the front end sheet being provided with a groove, and the groove containing liquid including biological tissue.

14. The biological tissue transfer device of claim 1, wherein, The application further relates to a device for transferring biological tissue, comprising: an automatic filling device for sucking external liquid into the sleeve and an electric control device for controlling the axial movement of the carrier rod or the sleeve.

15. The biological tissue transfer device of claim 1, wherein, The application further relates to a device for transferring biological tissue, comprising: a reset device for resetting the carrier rod or the sleeve.

16. The biological tissue transfer device of claim 1, wherein, The sleeve is a straw.

17. The biological tissue transfer device of claim 1, wherein, The biological tissue includes an embryo or an ovum.

18. A method of transferring biological tissue, comprising: The application further relates to a device for transferring biological tissue, comprising: a clamp, a carrier rod and a sleeve being clamped on the clamp respectively, the carrier rod containing liquid including biological tissue; one end of the carrier rod containing biological tissue being in contact with one end of the sleeve, wherein the carrier rod and / or the sleeve move relatively to each other to transfer the biological tissue in the carrier rod to the sleeve.

19. The biological tissue transfer method of claim 18, wherein, The carrier rod and / or the sleeve move relatively to each other in an axial direction.

20. The biological tissue transfer method of claim 18, wherein, The axial movement is an axial reciprocating movement.

21. The biological tissue transfer method of claim 20, wherein, The axial reciprocating movement is one or more acceleration movements.

22. The biological tissue transfer method of claim 21, wherein, Acceleration is generated by electromagnetic force.

23. The biological tissue transfer method of claim 19, wherein, The application further relates to a device for transferring biological tissue, comprising: the clamp drives the carrier rod to move in an axial reciprocating movement to transfer the biological tissue in the carrier rod to the sleeve.

24. The biological tissue transfer method of claim 18, wherein, The clamp comprises a clamp body, a first clamping part and a second clamping part, the first clamping part being movably connected to the clamp body. The method further comprises: clamping the carrier rod on the first clamping part and clamping the sleeve on the second clamping part, and allowing the first clamping part to axially move relative to the fixture body.

25. The biological tissue transfer method of claim 24, wherein, The fixture further comprises a slider arranged below the first clamping part, and the first clamping part has a locking structure for locking the carrier rod; the method further comprises: sliding the slider on the fixture body, and driving the first clamping part and the carrier rod clamped thereby to axially reciprocate.

26. The biological tissue transfer method of claim 24, wherein, Further comprising: arranging a docking part between the first clamping part and the second clamping part of the fixture, and the docking part comprises a horn-shaped part defining a docking position of the carrier rod and the sleeve.

27. The biological tissue transfer method of claim 26, wherein, The docking part comprises a positioning part, one side of the positioning part is provided with at least one carrier rod positioning hole, and the other side of the positioning part is provided with at least one sleeve positioning hole in communication with the at least one carrier rod positioning hole, and the at least one carrier rod positioning hole and the at least one sleeve positioning hole gradually expand from inside to outside in a horn shape; wherein the carrier rod positioned by the carrier rod positioning hole and the sleeve positioned by the sleeve positioning hole are in contact at the docking part.

28. The biological tissue transfer method according to claim 18 or 26, wherein, Further comprising: arranging one or more carrier rod supporting frames on the fixture, and each of the carrier rod supporting frames is used for carrying a plurality of carrier rods.

29. The biological tissue transfer method of claim 28, wherein, The number of the carrier rods is 1-3.

30. The biological tissue transfer method of claim 18, wherein, The carrier rod comprises a handle, a carrier rod body and a front end sheet, the front end sheet is provided with a recess, and the recess contains at least a liquid containing biological tissue.

31. The biological tissue transfer method of claim 18, wherein, Further comprising: sucking external liquid into the sleeve by an automatic filling device, and transferring biological tissue from the carrier rod into the liquid in the sleeve; and controlling the carrier rod or the sleeve to axially move by an electric control device, so as to contact one end of the carrier rod containing biological tissue with one end of the sleeve.

32. The biological tissue transfer method of claim 18, wherein, Further comprising: resetting the carrier rod or the sleeve by a resetting device.

33. The biological tissue transfer method of claim 18, wherein, The sleeve is a straw.

34. The biological tissue transfer method of claim 18, wherein, The biological tissue comprises an embryo or an ovum. The biological tissue comprises an embryo or an ovum.

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