Cosmetic composition for ameliorating acne, comprising extract of allium grayi regel or allium monanthum as effective component

Abstract

The present invention relates to a cosmetic composition for ameliorating or treating acne, comprising an extract of Allium grayi Regel or Allium monanthum as an effective component. The Allium grayi Regel and Allium monanthum extracts have an excellent effect of alleviating inflammation induced by Propionibacterium acnes, which is an acne-causing bacterium, and thus can be effectively used in cosmetics for ameliorating acne, or medicines or compositions for treating acne.

Description

Cosmetic composition for improving acne containing an extract of wild garlic or wild garlic as an active ingredient

[0001] The present invention relates to a composition for improving or treating acne comprising an extract of wild garlic or wild garlic as an active ingredient, and more specifically, to a cosmetic composition for improving acne containing an extract obtained from wild garlic or wild garlic.

[0002]

[0003] Acne is an inflammatory disease of the sebaceous glands and is a very common skin condition that causes inflammation in the hair follicles of the facial skin. The cause of acne originates from the abnormal function of androgen hormones; these hormones travel through the bloodstream to the sebaceous glands in the skin, stimulating oil production. This excessive secretion of oil fills the pores. Along with this excessive oil production, hyperkeratosis is accelerated at the pore opening, causing the opening to narrow or become blocked. As a result, the oil filling the pores stops flowing outward and becomes trapped and stagnant inside. These trapped oil clumps and impurities intertwine to form acne comedes. When the amount of stagnant oil within the pores increases, the bacterium Propionibacterium acnes becomes active. The free fatty acids produced as byproducts after the bacteria consume the oil components severely irritate the pores, triggering inflammation.

[0004] Generally, antimicrobial substances such as erythromycin, tetracycline, and cindamycin have been primarily used in the treatment of skin diseases caused by bacteria, such as acne or inflammation. However, these antimicrobial substances have caused many problems, including the development of resistance in acne-causing bacteria upon long-term use and side effects such as photosensitivity. Furthermore, Propionibacterium acnes, the causative bacterium of acne, is difficult to artificially culture, and there are issues with the inability to use drugs containing antibiotics as cosmetics. In response to this, research has recently been actively conducted to identify active ingredients derived from natural resources, including plants, that can reduce the aforementioned side effects, be used continuously, and possess a powerful anti-acne effect while being harmless to the human body.

[0005] Meanwhile, Allium grayi Regel is a perennial plant of the Liliaceae family of the genus Allium that contains saponins such as smilagenein, sarasapogenin, tigogenin, and diosgenin, and it has been confirmed that it enables pharmacological activities such as anticancer, antibacterial, antiasthmatic, antioxidant, and antidepressant effects.

[0006] Allium monanthum Max., also known as wild chives, is a perennial herbaceous plant belonging to the genus Allium of the Liliaceae family. It is a wild plant widely distributed in Northeast Asia, including Korea, Japan, China, and Mongolia. Wild chives are widely used not only for culinary purposes but also for medicinal use, and their main medicinal effects are known to include antibacterial, hemostatic, blood-replenishing, and nerve-stabilizing properties. Wild chives contain alliin, a plant component responsible for the onion scent resulting from the action of alliin-degrading enzymes found in garlic and onions (Allium sativum). Alliin is also referred to as 3-(2-propensulfinyl)-L-alanine or S-allyl-L-cysteine ​​sulfate. Additionally, methyl alliin and scorodose have been reported as components with antibacterial activity (Shanghai Science & Technology Press (1985) In "Encyclopedia of Chinese Drugs", pp. 226, Shogakukan, Tokyo, Japan). Regarding technologies related to Allium grayi, Korean Published Patent No. 2022-0019629 discloses an anti-obesity composition containing Allium grayi extract, and Korean Published Patent No. 2022-0003878 discloses a composition for the prevention, improvement, or treatment of prostate disease containing Allium grayi extract as an active ingredient. Additionally, regarding technologies related to Allium grayi, Korean Published Patent No. KR 2021-0076725A discloses a moisturizing composition containing Allium grayi extract as an active ingredient, but no composition for the improvement or treatment of acne containing Allium grayi Regel or Allium grayi as an active ingredient according to the present invention has been disclosed.

[0007]

[0008] The present invention was derived from the above-mentioned needs and aims to provide a composition effective in improving or treating acne by including an extract of wild garlic or wild garlic as an active ingredient.

[0009] In addition, the present invention aims to provide a composition that is non-cytotoxic and effective in improving or treating acne by suppressing inflammation induced by P. acnes, an acne-causing bacterium.

[0010] In addition, the purpose is to provide a composition for improving or treating acne that reduces the burden of side effects associated with existing chemical formulations by utilizing natural extracts.

[0011] To achieve the above technical objective, a cosmetic composition for improving acne according to one embodiment of the present invention comprises an extract of wild garlic or wild garlic as an active ingredient.

[0012] In addition, in a cosmetic composition for improving acne according to one embodiment of the present invention, the acne may be acne caused by Propionibacterium acnes.

[0013] In addition, in a cosmetic composition for improving acne according to one embodiment of the present invention, the content of the extract may be composed of 0.05 to 10 w% with respect to the total amount of the composition.

[0014] In addition, in a cosmetic composition for improving acne according to one embodiment of the present invention, the solvent of the extract may include water, a C1 to C4 lower alcohol, or a mixture thereof.

[0015] In addition, in a cosmetic composition for improving acne according to one embodiment of the present invention, the cosmetic composition may be composed of a formulation selected from the group consisting of a solution, a suspension, an emulsion, a paste, a gel, an essence, a cream, a lotion, an emulsion, a powder, a soap, a surfactant-containing cleansing product, an oil, a powder foundation, an emulsion foundation, a wax foundation, a stick, and a spray.

[0016] In addition, a health functional food composition for improving acne according to one embodiment of the present invention comprises an extract of wild chives or wild chives as an active ingredient.

[0017] In addition, a pharmaceutical composition for treating acne according to one embodiment of the present invention comprises an extract of wild garlic or wild garlic as an active ingredient.

[0018] In addition, the composition for alleviating acne inflammation according to one embodiment of the present invention may be inflammation induced by Propionibacterium acnes.

[0019]

[0020] According to the means for solving the above-described problem, the present invention includes an extract of wild garlic or wild garlic as an active ingredient and has an excellent effect in improving or treating acne.

[0021] In addition, the present invention has the effect of being useful in various compositions, such as cosmetics for acne improvement, functional foods, pharmaceutical compositions, and compositions for inflammation relief, by inhibiting inflammation induced by Propionibacterium acnes, an acne-causing bacterium, through the use of an extract of wild garlic or wild garlic.

[0022] In addition, the present invention has the effect of reducing the burden of side effects of existing compound formulations by using natural extracts.

[0023] The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description of the invention or the claims.

[0024] Figure 1 shows the results of confirming the effect of wild garlic extract (a) and wild garlic extract (b) on the cell viability (%) of human skin cells.

[0025] Figure 2 shows the results of confirming the antibacterial effects of wild garlic extract (a) and wild garlic extract (b) on the acne bacteria Propionibacterium acnes (P. acnes) strain at different concentrations. Compared to the negative control group (PC), the amount of IL-8 in the wild garlic extract and wild garlic extract was almost identical or similar, which confirms that there is a significant antibacterial effect against the acne bacteria.

[0026] Figure 3 shows the results of confirming the anti-inflammatory efficacy of wild garlic extract (a) and wild garlic extract (b) at different concentrations. Compared to the comparative example (NMMA) treated with an antioxidant, the NO production ratio of wild garlic extract and wild garlic extract was lower or similar, which confirms that the anti-inflammatory effect is superior.

[0027] The present invention will be described below with reference to the attached drawings. However, the present invention can be implemented in various different forms and is therefore not limited to the embodiments described herein.

[0028] Throughout the specification, when it is stated that a part is "connected (connected, in contact, combined)" with another part, this includes not only cases where they are "directly connected," but also cases where they are "indirectly connected" with other members interposed between them. Furthermore, when it is stated that a part "includes" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but rather allows for the inclusion of additional components.

[0029] The terms used herein are merely for describing specific embodiments and are not intended to limit the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, terms such as “comprising” or “having” are intended to indicate the presence of the features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, and should be understood as not precluding the existence or addition of one or more other features, numbers, steps, actions, components, parts, or combinations thereof.

[0030] The terms used in the present invention have their ordinary meanings in the relevant field, and unless specifically indicated otherwise, the following terms in the present invention have the meanings defined below.

[0031] In the present invention, "extract" refers to a substance obtained by extracting components of a natural product, regardless of the extraction method, extraction solvent, extracted components, or the form of the extract, and may include all substances that can be obtained by processing or treating the substance obtained by extracting components of a natural product through other methods after extraction.

[0032] The extraction method of the above extract may utilize physical, chemical, or enzymatic methods, such as steam extraction, steam distillation, solvent extraction, supercritical fluid extraction, mechanical pressing extraction, microwave extraction, or enzyme extraction.

[0033] In the present invention, the extract of wild garlic or wild garlic may include, respectively, a fruit extract, a stem extract, a leaf extract, or an underground part extract, and may include a whole plant extract.

[0034] The present invention will be described in detail below.

[0035] The present invention provides a composition for improving, preventing, and treating acne, comprising an extract of wild garlic or wild garlic as an active ingredient, and a method for preparing the same. Preferably, the acne is acne caused by Propionibacterium acnes, but is not limited thereto.

[0036] The above extract of wild chives or wild chives can be prepared by a method comprising the following steps.

[0037] (1) A step of extracting by adding an extraction solvent to wild garlic or wild garlic; and

[0038] (2) A step of filtering the extract of step (1) above;

[0039] (3) A step of preparing an extract by concentrating the filtered extract from step (2) under reduced pressure and drying it.

[0040] In step (1) above, the extraction solvent is preferably water, a C1 to C4 lower alcohol, or a mixture thereof, but is not limited thereto.

[0041] In the above manufacturing method, the extraction method of the extract may utilize any conventional method known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction. It is preferable to add the extraction solvent in an amount of 1 to 20 times the volume of the dried wild chives, and more preferably 3 to 10 times. The extraction temperature is preferably 20 to 50°C, but is not limited thereto. In addition, the extraction time is preferably 3 to 100 hours, more preferably 4 to 6 hours, and most preferably 5 hours, but is not limited thereto. In the above method, the vacuum concentration in step (3) is preferably performed using a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, drying is preferably performed by vacuum drying, vacuum drying, boiling drying, spray drying, or freeze-drying, but is not limited thereto.

[0042] The present invention may be a cosmetic composition comprising an extract of wild garlic or wild garlic as an active ingredient.

[0043] The above cosmetic composition preferably has a formulation selected from the group consisting of solutions, emulsions, suspensions, pastes, creams, gels, powders, sprays, surfactant-containing cleansers, lotions, soaps, oils, powder foundations, emulsion foundations, and wax foundations, but is not limited thereto. The cosmetic composition made of each of these formulations may contain various bases and additives that are necessary and appropriate for the formulation of the formulation, and the types and amounts of these ingredients can be easily selected by a person skilled in the art.

[0044] In the case where the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvating agent, or an emulsifying agent is used as a carrier component, such as water, purified water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

[0045] The formulation of an emulsion according to one embodiment of the present invention is a type of heterogeneous mixture in which liquid phase particles are uniformly spread and float in a liquid phase solvent, and the type may be a water-in-oil (W / O), oil-in-water (O / W), water-in-oil-in-water-in-oil (W / O / W), or oil-in-water-in-oil (O / W / O) type emulsion. According to one embodiment of the present invention, the emulsion may include preservative and moisturizer components that are commonly used in cosmetic compositions to be prepared and used in the form of an emulsion.

[0046] In the case where the formulation of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, purified water, ethanol, or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used as carrier components.

[0047] In the case where the formulation of the cosmetic composition of the present invention is a paste, cream, or gel, purified water, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component.

[0048] When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane-butane, or dimethyl ether.

[0049] In the case where the formulation of the cosmetic composition of the present invention is a cleansing product containing a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative, or ethoxylated glycerol fatty acid ester, etc. may be used as a carrier component.

[0050] The cosmetic composition of the present invention may additionally contain excipients including fluorescent substances, fungicides, hydrotropism-inducing substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunblocks, vitamins, plant extracts, etc.

[0051] In one embodiment of the present invention, when the cosmetic composition is prepared as a toner, lotion, skin lotion, etc., it may necessarily include a wild garlic extract or wild garlic extract; purified water; a moisturizer; a preservative; and an alcohol, and optionally may further include a skin conditioning agent, an oil, a surfactant, an auxiliary surfactant, a fragrance, a raw material byproduct, a metal ion chelating agent, etc.

[0052] In another embodiment of the present invention, when the cosmetic composition is prepared as an emulsion or lotion, essence, serum, cream, etc., it may necessarily include a wild garlic extract or wild garlic extract; purified water; a moisturizer; a preservative; an oil; a surfactant; and a thickening agent, and optionally may further include a skin conditioning agent, an auxiliary surfactant, a fragrance, a raw material byproduct, a metal ion chelating agent, etc.

[0053] In addition, those skilled in the art may select any additional components and / or amounts thereof so that the advantageous properties of the composition according to this specification are not adversely affected or are substantially affected by the expected addition.

[0054] The above wild chive extract and wild chive extract may be included as active ingredients to alleviate acne. In particular, they may have the effect of suppressing inflammation induced by Propionibacterium acnes, an acne-causing bacterium.

[0055] The above-mentioned wild chive extract or wild chive extract may be included in an amount of 0.05 to 10 w% based on the total weight of the composition, preferably in an amount of 0.05 to 5 w%. When the cosmetic composition is manufactured as a toner, lotion, skin lotion, etc., it is preferable that the content of the wild chive extract or wild chive extract be 0.05 to 5 w%. When the cosmetic composition is in the form of an emulsion, lotion, essence, serum, cream, etc., the extract content may be 10 w% or less due to high stability. If the wild chive extract or wild chive extract is used in an amount greater than the above range, there is a risk of skin irritation, and if it is used in an amount less than the above range, the acne-relieving effect may be negligible. In this case, it is preferable to include the wild chive extract or wild chive extract in the aqueous phase during the manufacturing process, but it is not limited thereto. For example, if necessary, a part or all thereof may be included in the ethanol phase or oil phase, etc., for manufacturing.

[0056] The cosmetic composition of the present invention may include purified water. The content of the purified water may be appropriately selected according to the formulation of the cosmetic composition, and the remainder may be filled with purified water. Preferably, when the composition is prepared as a toner, lotion, skin lotion, etc., it may contain 77 to 94 w%. And when the cosmetic composition is prepared as an emulsion, lotion, essence, serum, cream, etc., the content of the purified water may contain 57 to 83 w%.

[0057] The cosmetic composition of the present invention may include a moisturizer for moisturizing effects or skin barrier improvement. The types of moisturizers may include one or more of glycerin or butylene glycol, propylene glycol, dipropylene glycol, higher fatty acids, squalene, erythritol, sorbitol, and mixtures thereof, and preferably may include glycerin.

[0058] Meanwhile, if the above moisturizer is a cosmetic ingredient that performs complex functions such as moisturizing and preservation, whitening, skin soothing, protection, anti-inflammatory, homeostasis, anti-aging, and wrinkle improvement, it may be used as such for complex purposes according to the user's needs. The content of the above moisturizer may include 0.5 to 10.5 w% when the composition is manufactured as a toner, lotion, skin lotion, etc., and may include 7 to 20 w% when the composition is manufactured as an emulsion, lotion, essence, serum, cream, etc. If the above moisturizer is below the above range, the moisturizing effect according to each formulation is negligible, and if it exceeds the above range, there is a problem with poor formulation stability or usability.

[0059] The cosmetic composition of the present invention may include a preservative for preservation or as an auxiliary preservative. The said preservative may be included for the purpose of preservation or antibacterial action to ensure the safe long-term use of the cosmetic product. In particular, in addition to having the function of helping to improve the preservative power of the cosmetic product by facilitating the destruction of microbial cell membranes, it may also be included for complex purposes as it has moisturizing and solvent functions.

[0060] The above preservative may include one or more of 1,2-hexanediol, ethylhexylglycerin, dichlorobenzyl alcohol, phenoxyethanol, dehydroacetic acid, benzyl alcohol, benzoic acid, sodium benzoate, calcium benzoate, potassium benzoate, sorbic acid and its salts, sorbic acid, potassium sorbate, sodium sorbate and mixtures thereof, and the content thereof may be 1 to 3 w%. If the preservative is below the above range, the preservative effect is negligible, and if it exceeds the above range, there is a possibility of irritating the skin.

[0061] The cosmetic composition of the present invention may include oil. The oil helps to dissolve the oil phase components and firmly form an interfacial film, thereby allowing for the effect of delaying the evaporation of moisture from the skin surface. The oil may include plant-derived oils, dicaprylyl ether oils, mineral-derived oils, synthetic oils, ester oils, etc., which are commonly used in the relevant technical field.

[0062] The above oils are vegetable oil, olive oil (Olea Europaea (Olive) Fruit Oil), sunflower seed oil (Helianthus Annuus (Sunflower) Seed Oil), jojoba oil, meadowfoam seed oil, rice germ oil, passion fruit seed oil, Queensland nut oil, macadamia seed oil, sunflower seed oil, coconut oil, squalene, squalane, capric / caprylic triglyceride, zinc ricinoleate squalane, dicaprylyl ether, butylene glycol dicaprylate / dicaprate, propylene glycol dicaprylate / dicaprate, propylene glycol dicaprylate, Dicaprylyl Carbonate, Coco-caprylate / Caprate, Caprylyl Methicone, Cetyl Ethylhexanoate, Diglyceryl Triisostearate, Glyceryl Tri2-Ethylhexanoate, Isononyl Isononanoate, Ethylhexyl Isononanoate, Ethylhexyl Palmitate, Isostearyl Isostearate, Neopentyl Glycol Dicaprate, Neopentyl Glycol Diethylhexanoate, Octyldodecyl Myristate, Penta-Thrily Tetraethylhexanoate, Penta-Thrily Tetraisostearate, Isotridenyl Isononanoate, Trimethylopropane Triisostearate, Butyl Octyl Salicylate (Butyloctyl Salicylate), Cyclopentasiloxane, Dimethicone, Disiloxane, Trisiloxane, Ethyl Methicone, Methyl Trimethicone, Phenyl Trimethicone, Isododecane,It may contain at least one of isopropyl palmitate and mixtures thereof, and, exemplarily, vegetable oil may be used alone or in combination. Herein, the vegetable oil may include a pressed oil of plant origin composed of triglycerides of fatty acids.

[0063] The oil content may be in the range of 3 to 5 w%. If the oil content is below the above range, the effect is negligible, and conversely, if it exceeds the above range, it is difficult for the oil phase components to effectively form emulsified particles, which may reduce the dispersion and long-term stability of the formulation.

[0064] The cosmetic composition of the present invention may include a surfactant as an additional component to uniformly disperse aqueous and oil phase components. The surfactant is not particularly limited as long as it is a surfactant that can be used in a cosmetic composition, such as anionic surfactants or nonionic surfactants, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters and polyoxyethylene alkyl ethers, sorbitan fatty acid esters, glyceryl fatty acid esters, polyoxyethylene / polyoxypropylene alkyl ether block copolymers, bio-derived phospholipid and amino acid derivatives, polyglyceryl fatty acid esters, silicone-based surfactants, fluorinated surfactants, polymer emulsifiers, aliphatic alcohol emulsifiers, natural emulsifiers, etc.

[0065] Specifically, the surfactant is polyoxyethylene hydrogenated castor oil, castor oil, castor seed oil, hydrogenated castor oil, hydrogenated lecithin, hydrogenated palm glyceride, glyceryl monostearate, cetearyl alcohol, glyceryl stearate citrate, sorbitan trioleate, sorbitan tristearate, propylene glycol monostearate, sorbitan sesquioleate, sorbitan palmitate, sorbitan stearate, sorbitan isostearate, sorbitan sesquistearate, It may contain at least one of sorbitan oleate, sorbitan sesquioleate, sorbitan olivate, sucrose cocoate, methyl glucose sesquistearate, cetyl palmitate, cetearyl glucoside, decyl glucoside, coco-glucoside, C12-20 alkyl glucoside, arachidyl glucoside, and mixtures thereof, and preferably polyoxyethylene hydrogenated castor oil.

[0066] The above surfactant may be included in a range of 0.2 to 4 w%. Preferably, when the cosmetic composition is prepared as a toner, lotion, skin lotion, etc., it may be included in a range of 0.2 to 1 w%, and when the composition is prepared as an emulsion, lotion, essence, serum, cream, etc., it may be included in a range of 1 to 4 w%. If the content of the surfactant is below the above range, it may be difficult to properly emulsify it according to each formulation, and conversely, if it exceeds the above range, stickiness or off-odor may occur, which may reduce the user experience.

[0067] The cosmetic composition of the present invention may include a thickening agent. The thickening agent is for controlling viscosity and may use a polymer. The polymer may be selected from one or more of ammonium acryloyl dimethyl taurate / VP copolymer (Aristoflex AVC), hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, polyacrylate crosspolymer-6, carbomer, guar gum, xanthan gum, carboxymethylcellulose, hydroxyethylcellulose, sodium polyacrylate, hydroxypropylcellulose and sodium hyaluronate, polyacrylate, polyacrylamide and mixtures thereof, and preferably, ammonium acryloyl dimethyl taurate / VP copolymer (Aristoflex AVC) may be used.

[0068] The preferred content of the above-mentioned thickener may be 0.2 to 1 w%. If the content of the thickener is below the above range, it is difficult to see a viscosity-forming effect, and if it exceeds the above range, the formulation characteristics are degraded due to the increase in viscosity.

[0069] The cosmetic composition of the present invention may include alcohol for purposes such as preventing foam formation, acting as a solvent, or as an antibacterial agent. The alcohol is not limited to any alcohol used in cosmetic compositions, such as ethanol, dinatonium benzoate, t-butyl alcohol, diethyl phthalate, methyl alcohol, salicylic acid, sodium salicylate, or methyl salicylate.

[0070] The preferred content of the above alcohol may be 1 to 5 w%. If the alcohol content is below the above range, it is difficult to achieve foam prevention, solvent, and antibacterial effects, and if it exceeds the above range, the formulation characteristics may deteriorate, resulting in poor usability.

[0071] The cosmetic composition of the present invention may include a skin conditioning agent for skin soothing, skin barrier protection, and improved usability effects. The above skin conditioning agent is betaine, hyaluronic acid, hydrolyzed hyaluronic acid, sodium hyaluronate, panthenol, sodium PCA, pantolactone, adenosine, allantoin, cyclopentasiloxane, arginine, dipotassium glycyrrhizate, phytosterol, tocopheryl acetate, ascorbic acid, xylitol, anhydrous xylitol, butylene glycol, hydrogenated polydecene, niacinamide, ethylhexyl palmitate, polyglyceryl-3 methylglucose distearate, dipotassium glycyrrhizate, alpha-arbutin, RH-oligopeptide-1, sucrose distearate, ethylhexylglycerin, caprylyl glycol, pentylene glycol, glacial water, trideceth-10, shea butter, It may include one or more of fig extract, witch hazel extract, Polygonum multiflorum root extract, Scutellaria baicalensis extract, green tea extract, Spanish licorice root extract, Matricaria flower extract, rosemary leaf extract, Houttuynia cordata extract, Magnolia bark extract, Vulgaris vulgaris leaf / stem extract, Aloe vera leaf extract, white willow bark extract, Boswellia resin extract, Candida / Garcinia ferment, and mixtures thereof.

[0072] The above skin conditioning agent may be included in a range of 0.005 to 0.22 w% when the composition is prepared as a toner, lotion, skin lotion, etc., and may be included in a range of 0.005 to 0.02 w% when the composition is prepared as an emulsion, lotion, essence, serum, cream, etc. If the content of the skin conditioning agent is below the above range, it is difficult to obtain skin soothing and protective effects suitable for each formulation, and if it exceeds the above range, the characteristics of the formulation may deteriorate, resulting in a poor user experience.

[0073] The cosmetic composition of the present invention may include an auxiliary surfactant as an additional ingredient. The auxiliary surfactant may be a higher alcohol, and the higher alcohol may help form a lamellar structure at the interface, increase viscosity, and stabilize the formulation.

[0074] The above auxiliary surfactant is not particularly limited as long as it is generally used in cosmetic compositions, and preferably may be used including at least one of behenyl alcohol, cetyl alcohol, cetearyl alcohol, cetanol, stearyl alcohol, oleyl alcohol, isostearyl alcohol, 2-octyldodecyl, 2-ethylhexane cetyl, C14-22 alcohol, arachidyl alcohol, cetostearyl alcohol, batyl alcohol, and mixtures thereof, and more preferably may behenyl alcohol.

[0075] The above auxiliary surfactant may be included in an amount of 0.1 to 7 w%. If the content of the auxiliary surfactant is below the above range, it may not be able to perform the functions of increasing viscosity and stabilizing the formulation, and conversely, if it exceeds the above range, the user experience may be reduced.

[0076] The cosmetic composition of the present invention may include a fragrance to add scent to the product. The fragrance may be a natural substance, an artificial substance, or a mixture thereof. Examples of the fragrance include lavender or rosemary, tea tree, orange, lime, rose, wood, etc., and are not particularly limited as long as they are used in cosmetics.

[0077] The preferred content of the above fragrance may be 0.05 to 0.2 w%. If the content of the fragrance is below the above range, it is difficult to see a fragrance effect, and if it exceeds the above range, it may cause an unpleasant sensation.

[0078] In addition, the cosmetic composition according to one embodiment of the present invention may further include fragrances, raw material by-products, metal ion chelating agents, chelating agents, neutralizing agents, stabilizers, etc. as additional ingredients.

[0079] Next, the present invention may be a pharmaceutical composition for improving or treating acne comprising wild chive extract or wild chive extract as an active ingredient.

[0080] The above pharmaceutical composition may be in the form of a cream or gel, a patch, a spray, an ointment, a warning agent, a lotion, a cleaning agent, etc.

[0081] The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier, excipient, or diluent. The pharmaceutically acceptable carrier included in the pharmaceutical composition of the present invention is one that is commonly used in formulations and includes, but is not limited to, saline solution, sterile water, Ringer's solution, buffered saline solution, dextrose solution, maltodextrin solution, glycerol, ethanol, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

[0082] In addition, in addition to the above active ingredients, the composition may further include antioxidants, buffers, bacteriostatic agents, diluents, surfactants, binders, lubricants, humectants, sweeteners, flavorings, emulsifiers, suspending agents, or preservatives. The pharmaceutical composition of the present invention may be administered orally or parenterally, and parenteral administration may be performed by injection or by application to the skin. The appropriate dosage of the pharmaceutical composition of the present invention may be prescribed in various ways depending on factors such as the formulation method, mode of administration, patient's age, body weight, sex, pathological condition, food, time of administration, route of administration, excretion rate, and response sensitivity.

[0083] In addition, the present invention may relate to a composition for alleviating inflammation caused by Propionibacterium acnes, comprising a wild chive extract or a wild chive extract as an active ingredient.

[0084] The above composition may be in the form of a cream or gel, patch, spray, ointment, warning agent, lotion, cleaning agent, etc.

[0085] In addition, the present invention makes it possible to prepare a health functional food composition for improving acne by including a wild chive extract, a wild chive extract, or a fraction thereof as an active ingredient.

[0086] The health functional food composition of the present invention may be prepared by adding the active ingredient, the wild chive extract, wild chive extract, or a fraction thereof as is, or by mixing it with other foods or food ingredients, and may be appropriately prepared according to conventional methods. Examples of foods to which the wild chive extract, wild chive extract, or a fraction thereof may be added include any one form selected from caramel, meat, sausage, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and include all health functional foods in the conventional sense. That is, there are no special restrictions on the types of foods. The above health functional food composition may contain various nutritional supplements, vitamins, minerals (electrolytes), synthetic and natural flavoring agents, coloring agents and promoters (cheese, chocolate, etc.), pectic acid and its salts, alkinic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. Additionally, it may contain fruit pulp for the production of natural fruit juices and vegetable beverages. The above ingredients may be used independently or in combination. Furthermore, the health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates, etc. as additional ingredients, and the natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The proportion of the above natural carbohydrates is not of great importance, but it is preferable that it be 0.01 to 0.04g per 100g of the composition of the present invention, and more preferably 0.02 to 0.03g, but is not limited thereto. As a sweetener, natural sweeteners such as taumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

[0087] Next, a method for preparing a composition for improving or treating acne according to one embodiment of the present invention will be described. The method for preparing a composition according to the present invention is not particularly limited, and in the case of a formulation such as a toner, cosmetic water, or skin lotion, it may be composed of an aqueous phase and an ethanol phase component, and in the case of a formulation such as an emulsion, lotion, essence, serum, or cream, it may be composed of an aqueous phase component.

[0088] A method for preparing a cosmetic composition in the form of a toner according to one embodiment of the present invention is as follows.

[0089] 1) Dissolve purified water and aqueous raw materials in the first container using a mixer, and then prepare the aqueous phase at room temperature.

[0090] 2) Add an ethanol-phase raw material to the above aqueous phase and solubilize it for approximately 20 to 30 minutes.

[0091] 3) The cosmetic composition of the present invention is prepared through a cooling step.

[0092]

[0093] Next, a method for preparing an emulsion form according to one embodiment of the present invention will be described. The method for preparing a composition according to the present invention is not particularly limited, and a conventional method for preparing a cosmetic composition in the form of an emulsion may be used.

[0094] A method for preparing a cosmetic composition in the form of an emulsion according to one embodiment of the present invention is as follows.

[0095] 1) The aqueous phase is prepared by dissolving purified water and the aqueous raw material in the first container with a mixer and heating to 70 to 80°C.

[0096] 2) Mix the oil phase raw materials in the second container and heat to 70 to 80°C to produce the oil phase.

[0097] 3) After injecting the oil phase into the aqueous phase, the first mixture is prepared by first emulsifying at 70 to 80°C.

[0098] 4) After adding a polymer or the like to the first mixture, a second emulsification is performed at 55 to 70°C to prepare a second mixture.

[0099] 5) Add fragrances and other raw materials to the above secondary mixture and mix while heating to 40 to 50°C.

[0100] 6) The cosmetic composition of the present invention is prepared through a cooling step.

[0101]

[0102] Meanwhile, detailed components such as moisturizers, preservatives, surfactants, skin conditioning agents, co-surfactants, and fragrances described above may be manufactured by including them in aqueous and oil phases, polymer phases, ethanol phases, and other groups, taking into account the process. For example, fragrances may be manufactured by including part or all of them in the oil phase and / or ethanol phase, etc., as needed.

[0103]

[0104] Examples

[0105] The present invention will be described in more detail below using examples. These examples are solely for the purpose of more specifically explaining the present invention, and it is obvious to those skilled in the art that the scope of the present invention is not limited by them.

[0106]

[0107] 1-1: Preparation of Wild Garlic Extract

[0108] 15 L of 70% (v / v) ethanol was added to 1 kg of dried ground wild chives, extracted at 85°C for 3 hours, filtered, and then concentrated under reduced pressure and dried at 45°C to obtain wild chive extract.

[0109]

[0110] 1-2: Preparation of Wild Garlic Extract

[0111] Extraction was performed using the same method as the above-mentioned wild chive extract, but the wild chive was replaced with mountain wild chive to obtain the extract.

[0112]

[0113] Example 1: Preparation of a toner containing Allium victorialis extract

[0114] Next, the above-mentioned wild chive extract and the ingredients listed in Table 1 below were prepared in the following manner.

[0115] (1) After weighing the aqueous raw materials into a beaker according to the ingredients and content listed in Table 1, the aqueous phase was prepared at room temperature.

[0116] (2) After adding the ethanol phase to the above aqueous phase, the mixture was stirred and solubilized for 20 to 30 minutes.

[0117] (3) Example 1 of the present invention was prepared by cooling at room temperature.

[0118]

[0119] Ingredient Content (Weight%) Aqueous Substance: Purified Water 87.19g, Butylene Glycol 5.00g, Glycerin 2.00g, Hyaluronic Acid 0.01g, Preservative 2.00g, Betaine 0.10g, Wild Garlic Extract 0.10g Ethanol Substance: Ethanol 3.00g, Polyoxyethylene Hydrogenated Castor Oil 0.50g, Fragrance 0.10g

[0120]

[0121] Example 2: Preparation of an emulsion containing wild chive extract

[0122] Next, the above-mentioned wild chive extract and the ingredients listed in Table 2 below were prepared in the following manner.

[0123] (1) According to the ingredients and content listed in Table 2, the aqueous raw materials were weighed into a beaker and heated to 70~80℃ to prepare the aqueous part.

[0124] (2) After weighing the oil phase raw material into a beaker, the oil phase was heated to 70~80℃ to produce the oil phase.

[0125] (3) The above oil phase was mixed with the water phase to perform a first emulsion.

[0126] (4) A polymer phase was mixed and dispersed into the primary emulsified mixture to perform secondary emulsification.

[0127] (5) Added other ingredients (flavoring) to the secondary emulsified mixture and discharged at 30°C to complete Example 2.

[0128]

[0129] Ingredient Content (Weight%) Aqueous Phase: Purified Water 74.49g, Butylene Glycol 5.00g, Glycerin 10.00g, Hyaluronic Acid 0.01g, Preservative 2.00g, Allium Garlic Extract 0.1g, Oil Phase: Oils 4.00g, Surfactants 3.00g, Higher Alcohols 1.00g, Polymer Phase: Polymer 0.30g, Other Fragrances 0.10g

[0130]

[0131] Experiment 1-1: Confirmation of Cytotoxicity of Allium ursinum Extract

[0132] To confirm the cytotoxicity of the wild garlic extract on human keratinocytes (HaCaT), a CCK-8 (Cell Counting Kit-8) assay was performed. HaCaT cells were treated with the sample at different concentrations (25, 50, 100, 200 μg / ml) in a 96-well plate and cultured for 24 hours. After adding CCK-8 at a concentration of 10% and reacting at 37°C for 2 hours, the absorbance was measured at 450 nm using a microplate spectrophotometer (Biorad, Hercules, CA, USA) to calculate the cell viability (%).

[0133] As a result, as disclosed in Fig. 1(a), when the wild garlic extract was treated to HaCaT cell lines at concentrations of 50 and 100 μg / ml, there was almost no cytotoxicity, and even at a concentration of 200 μg / ml, the cytotoxicity was found to be negligible. Therefore, it can be acknowledged that the wild garlic extract has almost no cytotoxicity even at a concentration of 200 μg / ml and will not affect the skin.

[0134]

[0135] Experiment 1-2: Confirmation of Cytotoxicity of Allium monanthum Extract

[0136] Next, the cytotoxicity of the wild garlic extract was confirmed. The experiment was conducted in the same manner as the above wild garlic extract experiment, and as a result, it was confirmed that there was almost no cytotoxicity at concentrations of 50, 100, and 200 μg / ml of wild garlic extract (see Fig. 1(b)). Therefore, it can be acknowledged that the wild garlic extract also has almost no cytotoxicity even at a concentration of 200 μg / ml and does not affect the skin.

[0137]

[0138] Experiment 2-1: Confirmation of the anti-inflammatory effect of Allium ursinum extract on acne bacteria

[0139] Next, an experiment was conducted to confirm the effect of Allium monanthum extract on alleviating inflammation caused by acne bacteria. P. acnes KCTC 3314 was used as the acne bacterium. To conduct the experiment under anaerobic conditions, the bacteria were cultured in RC liquid medium (Reinforced Clostridial Medium, Difco, USA) for 48 hours. The cultured bacteria were centrifuged at 1,800 ×g for 5 minutes and washed twice with PBS (pH 7.4). After resolving the washed bacteria in PBS, heat-treated acne bacteria were prepared by heating at 80°C for 30 minutes. Human keratinocytes were placed in a 24-well plate at a concentration of 5 × 10⁶ 4 Cells were cultured at a concentration of cells / well, and the medium was replaced with serum-free medium after 24 hours. Then, the medium was pretreated with Allium victorialis extract at different concentrations (200, 100, 50 µg / mL) for 30 minutes, and P. acnes was cultured at a concentration of 1 × 10⁶ 5 The samples were treated with CFU / mL and cultured for 24 hours. Subsequently, the centrifuged culture medium was used to induce the expression of inflammatory cytokines, and the expression level of IL-8 (Interleukin-8) produced was measured using an ELISA kit. Here, a lower amount of IL-8 can be interpreted as a significant reduction in inflammation.

[0140] The above results regarding the expression amount of IL-8 (Interleukin-8) are explained with reference to Figure 2(a). As a result of measuring the amount of IL-8 in a negative control group (BLK) without any treatment after culturing acne bacteria, a comparative example (PC, Positive control) in which the amount of IL-8 was measured after treatment with EGCG(-)-epigallocatechin-3-gallate (EGCG), which is widely known for its antioxidant properties, at a concentration of 5 μM, and the amount of IL-8 after treatment with wild chive extract at different concentrations (200, 100, 50 μg / ml), it can be confirmed that the IL-8 expression amount decreases with increasing concentration of wild chive extract.

[0141] In conclusion, it was confirmed that treatment with wild garlic extract significantly reduced the expression of IL-8, and in particular, it was confirmed that starting from a concentration of 100 μg / ml, it had an effect equivalent to or greater than that of treatment with an antioxidant. Therefore, it can be acknowledged that wild garlic extract has an effect of alleviating acne inflammation.

[0142]

[0143] Experiment 2-2: Confirmation of the Anti-inflammatory Effect of Wild Garlic Extract on Acne Bacteria

[0144] Next, an experiment was conducted to confirm the effect of wild chive extract on alleviating acne bacteria inflammation. The experiment was conducted at the same concentration and using the same method as the above experiment on wild chive extract. As a result, as shown in Figure 2(b), it was confirmed that wild chive extract also significantly reduced the expression of IL-8, and it was found that from a concentration of 100 μg / ml, the levels were similar to those of the antioxidant treatment. Therefore, it can be acknowledged that wild chive extract also has an effect on alleviating acne inflammation.

[0145]

[0146] Experiment 3-1: Evaluation of Anti-inflammatory Efficacy of Allium ursinum Extract

[0147] Next, an experiment was conducted to verify the anti-inflammatory effect of the Allium ursinum extract by confirming the reduction in stimulation induced by Gram-negative bacteria. The anti-inflammatory effect was confirmed by examining changes in the amount of NO produced by RAW 264.7 cells stimulated with LPS (lipopolysaccharide). First, RAW 274.7 cells, seeded at appropriate concentrations in a 48-well plate, were treated with LPS (400 ng / ml). Then, Allium ursinum extract was added at different concentrations (100, 50, and 25 µg / ml) and cultured for 24 hours. After collecting the culture supernatant, the amount of NO was measured using Griess reagent according to the manufacturer's instructions. A decrease in NO production can be interpreted as a reduction in the number of bacteria.

[0148] Referring to Figure 3(a), the above NO production amount was measured in a negative control group (BLK) in a normal state without stimulation, in a first comparative example (LPS) in a state where stimulation was induced, in a second comparative example (NMMA, NG-methl-L-arginine acetate salt) in a state where stimulation was induced and then treated with a general antimicrobial agent at a concentration of 30 μM, and in a state where stimulation was induced and then treated with the wild garlic extract at different concentrations (100, 50, 25 μg / ml). As a result, it was confirmed that the amount decreased depending on the concentration of the wild garlic extract.

[0149] In conclusion, it was confirmed that the use of wild garlic extract significantly reduced NO production, and in particular, it was confirmed that wild garlic extract had an effect equivalent to or better than that of NMMA treatment starting from a concentration of 50 μg / ml. Therefore, it can be acknowledged that wild garlic extract has an anti-inflammatory effect.

[0150]

[0151] Experiment 3-2: Evaluation of Anti-inflammatory Efficacy of Wild Garlic Extract

[0152] Next, an experiment was conducted to confirm the anti-inflammatory effect of the wild chive extract. The experiment was conducted using the same evaluation method at the same concentration as the wild chive extract mentioned above. As a result, referring to Figure 3(b), it was confirmed that the amount of NO produced decreased with increasing concentration of the wild chive extract.

[0153] In conclusion, it was confirmed that the use of wild chive extract significantly reduced NO production, and that it had an effect equivalent to or better than treatment with NMMA starting from a concentration of 50 μg / ml. Therefore, it can be acknowledged that wild chive extract also has an anti-inflammatory effect.

[0154]

[0155] 4. Summary

[0156] To summarize the effects of the present invention, it can be seen that the wild garlic extract and wild garlic extract have no cytotoxic effects even at a concentration of 200 μg / ml, and have a significant effect in alleviating inflammation caused by acne bacteria P. acnes even at concentrations of 100 and 50 μg / ml, and also have excellent anti-inflammatory functional effects.

[0157] As described above, it will be understood by those skilled in the art that the present invention may be implemented in various modified forms without departing from the technical and essential characteristics of the invention, and the disclosed manufacturing and test examples should be considered not in a limiting sense, but in an illustrative sense to facilitate a better understanding of the invention. For example, each component described as a single unit may be implemented in a distributed or divided form, and likewise, components described as distributed or divided may be implemented in a combined form within the scope of understanding of those skilled in the art.

[0158] The scope of the present invention is defined by the claims set forth below, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts thereof should be interpreted as being included within the scope of the present invention.

Claims

1. A cosmetic composition for improving acne characterized by containing an extract of wild garlic or wild garlic as an active ingredient.

2. A cosmetic composition for improving acne according to claim 1, characterized in that the acne is acne caused by Propionibacterium acnes.

3. A cosmetic composition for improving acne, characterized in that, in claim 1, the extract is included in an amount of 0.05 to 10 w% relative to the total amount of the cosmetic composition.

4. A cosmetic composition for improving acne, characterized in that, in claim 1, the solvent of the extract is water, a C1-C4 lower alcohol, or a mixture thereof.

5. A cosmetic composition for improving acne according to claim 1, characterized in that the cosmetic composition has a formulation selected from the group consisting of a solution, suspension, emulsion, paste, gel, essence, cream, lotion, emulsion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, stick, and spray.

6. A health functional food composition for improving acne characterized by containing an extract of wild garlic or wild garlic as an active ingredient.

7. A pharmaceutical composition for treating acne characterized by containing an extract of wild garlic or wild garlic as an active ingredient.

8. A composition for alleviating inflammation induced by Propionibacterium acnes, characterized by containing an extract of wild garlic or wild garlic as an active ingredient.

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