Integration of a scrape sample into a cartridge system

Abstract

The present invention relates to a system for collecting and storing a skin sample of a test subject, the system comprising a collection device comprising an engaging segment, said engaging segment configured to collect the skin sample from the test subject; and a cartridge configured to receive the skin sample after collection The present invention also relates to a corresponding method and use of the system.

Description

[0001] Integration of a scrape sample into a cartridge system

[0002] The present invention generally relates to automated diagnostics for dermatology, in particular at the point-of-care.

[0003] The accurate collection and analysis of biological samples are critical in various fields, including clinical diagnostics, biomedical research, and forensic science. One common method of sample collection involves scraping the surface of biological tissues, such as skin, to obtain cells or other analytes for subsequent analysis. These scrape samples are often used to diagnose skin conditions, detect infections, or monitor disease progression.

[0004] US 2004 / 0219537 Al discloses an epidermal collection method, kit, and device.

[0005] Traditional methods for handling and processing scrape samples typically require multiple manual steps, including transferring the sample to a separate container, adding reagents, and ensuring proper mixing or agitation. These procedures can be cumbersome, timeconsuming, prone to contamination and manual pipetting errors and degradation of analyte, which can compromise the accuracy and reliability of the analysis.

[0006] To address these challenges, there is a growing need for integrated systems that simplify the collection, processing, and analysis of skin samples.

[0007] The present invention relates to a system for collecting and storing a skin sample of a test subject. The system comprises a collection device comprising an engaging segment, said engaging segment configured to collect the skin sample from the test subject. The system further comprises a cartridge configured to receive the skin sample after collection.

[0008] The skin sample can be used in the singular or plural. That is, the skin sample may contain a single skin piece or it may contain multiple skin pieces.

[0009] The skin sample may comprise single cells of the skin, cells in association of the skin and / or skin tissue.

[0010] That is, as used herein, the term "skin sample" can refer to epithelial and integumentary tissues, including but not limited to epidermis, mucosa (e.g., buccal mucosa, anal mucosa, vaginal mucosa, tongue epithelium), skin and skin appendages, and nail material or cuticle. The engaging segment may thus contact a surface of the skin of the test subject for collection of the skins sample the surface.

[0011] The engaging segment may comprise a diameter of at least 0.1 mm and at most 2 mm, preferably of at least 0.5 mm and at most 1.5 mm, such as, 1 mm. This can be particularly the case when the collection device may be a curette and / or when the engaging segment comprises a spoon-like shape, which will be discussed further below. Such dimensions can allow for a sufficient amount of skin sample to be collected, while reducing the risk of leaving a scar on the test subject.

[0012] The collection device may be a curette. The curette can be advantageous as it can be a particularly simple collection device, thus reducing complexity of the system. It may also be straightforward to use, thus alleviating the need of user expertise for collecting the skin sample.

[0013] The engaging segment may be configured to collect the skin sample by scraping it from the test subject. For example, the engaging segment may comprise a sharp edge for scraping the skin sample from the test subject. This may provide an easy way for collecting the skin sample, thus, alleviating the need of user expertise.

[0014] The engaging segment can comprise grooves and wherein the collection device may be configured such that the skin sample may be collected in the grooves. The grooves may be configured such that during scraping on a skin of the test subject, skin samples may be collected in the grooves. Thus, the grooves can be advantageous as they may facilitate skin sample retention on the collection device. Additionally, the grooves may also allow for a larger amount of skin sample to be collected and / or retained.

[0015] The grooves can comprise a depth of at least 1 pm and at most 1000 pm.

[0016] The engaging segment can comprise an abrasive surface and wherein the abrasive surface may be configured to collect the skin sample from the test subject via abrasion. This may alleviate the use of sharp edges on the engaging segment, thereby increasing safety of the engaging segment. That is, the abrasive surface may provide a particularly safe and easy to use collection device.

[0017] The collection device may be a swab or a brush. This may alleviate the use of sharp edges on the engaging segment, thereby increasing safety of the engaging segment. That is, the swab may provide a particularly safe and easy to use collection device. The engaging segment can comprise a tip which may be configured to absorb, collect, or transfer substances from a surface.

[0018] The tip may be made of cotton, foam and / or synthetic fibers, such as, nylon.

[0019] The engaging segment can comprise an adhesive surface. This may alleviate the use of sharp edges on the engaging segment, thereby increasing safety of the engaging segment. That is, the swab may provide a particularly safe and easy to use collection device.

[0020] The collection device can comprise a handle allowing a user to handle the collection device. The handle may thus increase ergonomics of the collection device.

[0021] The engaging segment may be configured, in use, to contact the test subject.

[0022] The engaging segment can comprise an abrasive and / or cutting edge.

[0023] The engaging segment may be configured to loosen, such as, separate, the skin sample from the test subject.

[0024] The collection device may be configured to retain the skin sample after collection.

[0025] The collection device may be configured to retain the skin sample via friction.

[0026] That is, the collection device may be configured such that the skin sample can be attached, preferably via friction, on an outer surface of the collection device. On the one hand this may allow the collection device to retain the skin sample. On the other hand, it may allow for the skin sample to be dislodged with ease.

[0027] The collection device may be configured to retain the skin sample adhered to the engaging segment. That is, the skin sample can get stuck on the engaging segment during collection.

[0028] The collection device may be configured to allow the skin sample to be dislodged from the collection device, e.g., via at last one of: shaking the collection device, wiping the collection device on a surface and washing the collection device in a liquid or gel. In other words, the collection device may be configured such that the skin sample is not permanently attached to the collection device. The cartridge can comprise a cartridge container configured to contain the skin sample. The cartridge container can preferably be configured for long term retention of the skin sample. For example, the skin sample may be contained in the cartridge container for days or weeks. This is different from the collection device which can typically be configured to retain the skin sample for a short term, such as, e.g., at most 10 minutes.

[0029] The cartridge can comprise a cartridge inlet configured to allow the skin sample enter the cartridge.

[0030] The cartridge can comprise a cartridge lid and wherein the cartridge lid may be configured to assume an open position, in which the cartridge lid may be configured to allow access to the cartridge inlet and a closed position, in which the cartridge lid may be configured to block access to the cartridge inlet. The cartridge lid may facilitate isolating an inner volume of the cartridge, in particular of the cartridge container, from an external environment.

[0031] The cartridge can comprise a cartridge body and wherein the cartridge inlet allows access inside the cartridge body.

[0032] The cartridge may be configured to perform at least one sample processing step to the skin sample while the skin sample may be inside the cartridge body. The sample processing step may comprise: lysing the skin sample; purifying the skin sample; amplifying target molecules in the skin sample, e.g., via PCR (Polymerase Chain Reaction), LAMP (Loop- mediated Isothermal Amplification), and RPA (Recombinase Polymerase Amplification); reading a signal (typically fluorescent light) from the sample, calculating a cycle threshold value, performing an analysis using a machine learning algorithm to calculate a probability score for a skin disease, based on these Ct values or raw fluorescence measurements,

[0033] The system further can comprise a transfer kit and wherein the transfer kit may be configured to receive the skin sample from the collection device and to provide the skin sample to the cartridge. The transfer kit may thus facilitate transferring the skin sample from the collection device to the cartridge. The transfer kit may provide means for safely storing the skin sample during the transfer from the collection device to the cartridge, such that the skin sample does not deteriorate. The transfer kit may also adapt to various collection devices, thus enabling the system to utilize different types of collection devices, without the need of adapting the cartridges. The transfer kit can comprise a transfer container configured to contain the skin sample. The transfer container may be configured to substantially isolate the skin sample from an external environment.

[0034] The transfer container may be a test tube.

[0035] The transfer kit may be configured to receive the skin sample from the collection device into the transfer container.

[0036] The transfer container can comprise a transfer container opening and wherein the transfer kit may be configured to receive the skin sample in the transfer container through the transfer container opening.

[0037] The transfer container may contain a receiving medium and wherein the transfer kit may be configured to receive the skin sample within the receiving medium to form a sample mixture, said sample mixture comprising the skin sample and the receiving medium.

[0038] The receiving medium may be a liquid or a gel. Generally, a liquid with a high viscosity or a gel may better dislodge the skin sample from the collection device, by providing a higher friction.

[0039] The transfer kit may be configured to receive a received portion of the collection device in the transfer container such that relative motion between the collection device and the transfer container may be allowed while the received portion may be received in the transfer container. Said relative motion can be advantageous for dislodging the skin sample from the collection device.

[0040] Relative motion between the transfer container and the collection device, while the received portion may be received in the transfer container, can cause the skin sample to be dislodged from the collection device.

[0041] The received portion can comprise the engaging segment. That is, the engaging segment can be received in the transfer container. This can be advantageous as the skin sample can typically be retained on the engaging segment. Thus, by inserting the engaging segment in the transfer container, i.e., by immersing the engaging segment in the receiving medium, friction with the receiving medium may disclosed the skin sample from the engaging segment. The skin sample may be retained in or on the received portion after collection.

[0042] The received portion may be submerged, i.e., immersed, at least in part in the receiving medium during the relative motion.

[0043] The transfer kit can comprise a transfer cap configured to attach, preferably to releasably attach, to the transfer container.

[0044] The transfer cap may be configured to cover the transfer container opening. This may prevent debris from entering inside the transfer container.

[0045] The transfer cap can comprise a dispensing tip, wherein the dispending tip can comprise a dispensing orifice. The dispensing tip can be advantageous as it may allow a dispensing the sample mixture in controlled manner from the transfer container.

[0046] The dispensing tip can comprise a tip protruding section, wherein the tip protruding section tapers to a tip end such that the cross-sectional area of the dispensing tip decreases towards the tip end.

[0047] The dispensing orifice may be located on the tip end.

[0048] The cartridge may be configured to receive at least a portion of the sample mixture. This way the skin sample can be transferred to the cartridge.

[0049] The cartridge and the transfer kit may be configured such that the skin sample may be transferable from the transfer kit to the cartridge by providing, e.g., by dripping, at least a portion of the sample mixture from transfer kit into the cartridge container.

[0050] The cartridge container can comprise a hydrophilic coating or an absorbent material configured to absorb the sample mixture.

[0051] The cartridge may be configured to receive the sample mixture into the cartridge container via the cartridge inlet.

[0052] The transfer kit may be configured to receive the skin sample from the collection device into the transfer cap and from the transfer cap into the transfer container. This can be advantageous as the cap may be easier to handle and therefore the process of providing the skin sample from the collection device to the transfer container may be easier. Moreover, the transfer container may typically contain a receiving medium. Thus, handling the transfer cap instead of the transfer container may advantageously alleviate spilling the receiving medium.

[0053] The transfer kit may be configured to receive the skin sample from the transfer cap into the transfer container during and / or after attaching the transfer cap to the transfer container. For example, the skin samples may fall (e.g., due to gravity) into the transfer container. Alternatively, or additionally, after attaching the transfer cap to the transfer container, the transfer container with the transfer cap may be shaken. This may facilitate dislodging of the skin samples from the transfer cap, particularly, if the transfer container contains the receiving medium.

[0054] The cartridge lid can comprise the cartridge container. The lid in the closed position may position the cartridge container in a predefined position inside the cartridge.

[0055] The cartridge container may be integrated in the cartridge lid.

[0056] The cartridge may be configured to receive the skin sample from the collection device into the cartridge container.

[0057] The cartridge container can comprise a cartridge container opening and wherein the cartridge may be configured to receive the skin sample from the collection device into the cartridge container through the cartridge container opening.

[0058] The cartridge lid may be configured such that in the open position the cartridge container opening faces away from the cartridge inlet and in the closed position the cartridge container opening faces towards the cartridge inlet.

[0059] The cartridge container may be dry while containing the skin sample. That is, no receiving medium may be contained inside the cartridge container.

[0060] The cartridge lid can comprise a lid cover and wherein the lid cover may be configured to assume an open position, in which the lid cover may be configured to allow access to the cartridge container and a closed position, in which the lid cover may be configured to block access to the cartridge container.

[0061] The lid cover may be configured to seal the cartridge container in the closed position. The lid cover may be configured to allow transitions from the closed position to the open position and from the open position to the closed position.

[0062] The lid cover may be elastic.

[0063] The lid cover may be configured to be transitioned from the closed position to the open position and from the open position to the closed position via deformation.

[0064] The lid cover can comprise a foil, such as, an aluminum foil.

[0065] The lid cover can comprise a peripheral section and a central section, wherein the peripheral section surrounds the central section and wherein the peripheral section may be configured to contact a peripheral wall of the cartridge container.

[0066] The peripheral section of the lid cover may be configured to attach to the peripheral wall of the cartridge container upon contact.

[0067] A surface of the peripheral section contacting the peripheral wall can comprise an adhesive coating.

[0068] Thus, the lid cover may easily seal the cartridge container.

[0069] The peripheral wall of the cartridge container can comprise a flat surface configured to coincide with the peripheral section of the lid cover when the lid cover may be in the closed position. The flat surface may provide for a robust attachment or adhesion of the peripheral section of the lid cover. Overall, this may provide a better sealing.

[0070] Said flat surface of the peripheral wall can comprise an adhesive coating.

[0071] The cartridge may be configured to receive the skin sample from the collection device by transitioning the lid cover from the closed position to the open position and dislodging the skin sample from the collection device into the cartridge container and transitioning the lid cover from the open position to the closed position. It will be understood that if the lid cover can be provided in the open position, then there may be no need to transition the lid cover from the closed position to the open position. The cartridge container may contain a receiving medium and wherein the cartridge may be configured to receive the skin sample within the receiving medium to form a sample mixture, said sample mixture comprising the skin sample and the receiving medium.

[0072] The cartridge may be configured to receive a received portion of the collection device in the cartridge container such that relative motion between the collection device and the cartridge container may be allowed while the received portion may be received in the cartridge container. Said relative motion can be advantageous for dislodging the skin sample from the collection device.

[0073] Relative motion between the cartridge container and the collection device, while the received portion may be received in the cartridge container, causes the skin sample to be dislodged from the collection device.

[0074] The received portion can comprise the engaging segment. That is, the engaging segment can be received in the cartridge container. This can be advantageous as the skin sample can typically be retained on the engaging segment. Thus, by inserting the engaging segment in the cartridge container, i.e., by immersing the engaging segment in the receiving medium, friction with the receiving medium may disclosed the skin sample from the engaging segment.

[0075] The skin sample may be retained in or on the received portion after collection.

[0076] The received portion may be submerged at least in part in the receiving medium during the relative motion.

[0077] The system can further comprise a transfer surface configured to receive the skin sample from the collection device and to support the skin sample thereon.

[0078] The system can further comprise a surface cover configured to cover the transfer surface.

[0079] The surface cover can comprise a sample collecting side configured to facilitate adhesion of the skin sample thereon.

[0080] The surface cover and the cartridge lid may be configured to attach to each other such that when the cartridge lid, with the surface cover attached thereon, may be in the closed position the surface collecting side faces the cartridge inlet. The transfer kit can comprise a gathering tool configured to collect the skin sample contained in the transfer container.

[0081] The transfer kit may be configured such that the gathering tool may be receivable, at least in part, in the transfer container such that relative motion between the gathering tool and the transfer container may be allowed while the gathering tool may be received, at least in part, in the transfer container.

[0082] The transfer kit may be configured such that relative motion between the gathering tool and the transfer container, while the gathering tool may be received, at least in part, in the transfer container, allows the gathering tool to collect the skin sample contained in the transfer container.

[0083] The cartridge may be configured to receive the skin sample from the gathering tool.

[0084] The gathering tool can comprise a gathering section configured to facilitate adhesion of the skin samples to the gathering section.

[0085] The gathering section may be removable from the gathering tool.

[0086] The gathering section may be at an end of the gathering tool.

[0087] The gathering section may be configured to be dissolvable in a liquid or gel, such as, the receiving medium. That is, the gathering section may be dissolvable in the receiving medium. In particular, the gathering section may be dissolvable in the receiving medium contained in the cartridge container.

[0088] The cartridge may be configured to receive the gathering section.

[0089] The cartridge may be configured to dissolve the gathering section with the skin sample attached thereto.

[0090] The cartridge may further be configured to purify the skin sample and, in particular, nucleic acids in the skin sample.

[0091] The system can further comprise homogenizing particles within the receiving medium configured to dissect and / or lyse the skin sample received within the receiving medium upon agitation of the homogenizing particles to thereby homogenize the sample mixture. Homogenizing the sample mixture may facilitate handling and / or processing the skin sample.

[0092] The homogenizing particles can be agitated via mechanical agitation of the transfer container and / or of the cartridge container.

[0093] The homogenizing particles can be agitated via stirring of the sample mixture.

[0094] At least some of the homogenizing particles may be configured to be magnetically agitated.

[0095] At least some of the homogenizing particles may be configured to be agitated via sonication.

[0096] The transfer kit can comprise a mortar tool configured to press the skin sample within the receiving medium to thereby homogenize the sample mixture.

[0097] The receiving medium can comprise reagents configured to inhibit skin sample degradation, preferably configured to inhibit DNA or RIMA degradation.

[0098] The receiving medium can comprise chaotropic agents.

[0099] The receiving medium can comprise release agents.

[0100] The receiving medium can be a liquid or a gel.

[0101] The cartridge may be configured to receive the skin sample from the collection device by dislodging the skin sample from the collection device onto a surface of the cartridge lid facing the cartridge inlet and transitioning the cartridge lid from the open position to the closed position.

[0102] The cartridge container can comprise a sample compartment configured to accommodate a metered volume of the sample mixture.

[0103] The sample compartment can comprise an absorbent material configured to absorb the sample mixture.

[0104] The metered volume may be at least 1 microliter and at most 3 milliliters. The cartridge container can comprise an outlet configured to allow the sample mixture exit the sample compartment.

[0105] The cartridge container can comprise an overflow compartment configured to accommodate excess sample mixture. The overflow compartment may facilitate storing excess sample mixture for further use.

[0106] The overflow compartment may be configured to accommodate an overflow volume of the sample mixture that overflows from the sample compartment.

[0107] The sample compartment and the overflow compartment may be provided adjacent to each other. Moreover, the cartridge container can comprise a metering wall, provided between the sample compartment and the overflow compartment. Further still, a dimension of the metering wall can determine the metered volume of the sample mixture. This can provide a simple metering mechanism.

[0108] The cartridge container may be configured such that an overflow volume of the sample mixture may be allowed to flow over the metering wall from the sample compartment to the overflow compartment.

[0109] The cartridge can comprise an inlet barrier.

[0110] The inlet barrier may be formed along a perimeter of the cartridge inlet.

[0111] The inlet barrier may be configured to prevent liquid or gel positioned next to the cartridge inlet from entering the cartridge.

[0112] The test subject may be a patient or an animal.

[0113] The transfer kit may be configured to lyse and / or fixate the skin sample.

[0114] The cartridge may be configured to lyse and / or fixate the skin sample.

[0115] The receiving medium may be configured to lyse and / or fixate the skin sample.

[0116] The cartridge may be configured to provide a metered volume of the skin sample and / or of a sample mixture comprising the skin sample to a downstream analysis device configured to analyze the skin sample. The cartridge may be configured to be received by a downstream analysis device configured to analyze the skin sample.

[0117] The system further can comprise the analysis device.

[0118] The analysis device may be configured to determine a cycle threshold value.

[0119] The analysis device may be configured to determine an amount of a target gene in the skin sample.

[0120] The analysis device may be configured to determine a probability scope for a skin disease.

[0121] The engaging segment can comprise a spoon-like shape. Said shape can facilitate effective collection of dislodged skin samples by providing a concave surface that can help retain the sample.

[0122] The engaging segment can further comprise a sharp edge configured to dislodge the skin sample from the test subject. The presence of a sharp edge can allow for precise and efficient sample collection with minimal applied force, which may reduce discomfort for the test subject.

[0123] The sharp edge can be circular or elliptical, preferably with a diameter of at least diameter of at least 0.1 mm and at most 2 mm, preferably of at least 0.5 mm and at most 1.5 mm, such as, 1 mm. A rounded shape can provide a more uniform scraping action, potentially minimizing localized pressure points and reducing the risk of skin irritation.

[0124] The engaging segment can further comprise a concave section defining a collection space, wherein the concave section can end at the sharp edge and can be configured to collect the skin sample within the collection space. The concave section can comprise a depth of at least 0.5 mm and at most 1.5 mm, such as, 1 mm. This configuration can help direct the sample into the collection space immediately upon dislodging, potentially preventing accidental dispersion and improving sample handling for subsequent handling of the skin sample.

[0125] The collection space can be accessible through the sharp edge. This can allow for direct transfer of the dislodged skin sample into the collection space. The sharp edge and the concave section can be configured to perform both dislodging and collection of the skin sample in a single scraping action. This can enhance operational efficiency by consolidating the sampling steps into a seamless motion.

[0126] The collection space can be dimensioned to accommodate a predetermined volume of the skin sample. By controlling the volume capacity, the system can ensure that an adequate amount of sample is collected, optimizing downstream processing while potentially preventing oversampling that could lead to unnecessary tissue disruption.

[0127] The engaging segment can be formed as an integral component of the collection device or can be detachably mounted to a handle. The integral design can enhance durability and reliability. Conversely, the detachable configuration can offer flexibility by allowing for easy replacement of the engaging segment, thereby extending the usability of the collection device and reducing operational costs.

[0128] As explained, the system can comprise a cartridge lid. In such embodiments, the cartridge lid can comprise an adhesive element. The cartridge lid can be configured to receive a skin sample adhered to the adhesive element from the collection device. This configuration can facilitate a direct and secure transfer of the sample from the collection device to the cartridge, reducing the risk of sample loss or contamination.

[0129] The cartridge lid can further comprise an underside surface that faces toward the cartridge body when in the closed position, with the adhesive element provided on the underside surface, preferably on a peripheral portion thereof. Positioning the adhesive element on the underside surface allows introduction of the skin sample inside the cartridge body by closing the cartridge lid. Moreover, positioning the adhesive element on the peripheral portion of the underside surface of the cartridge lid can facilitate effective contact between the adhesive element and a liquid or gel contained within the cartridge body (discussed below). Due to centrifugal forces, when the cartridge is rotated, the liquid or gel is pushed radially outward. If only a small amount of liquid or gel is present, a sample positioned too far inward may not be adequately wetted. By placing the adhesive element on the peripheral portion, the sample is positioned where the liquid or gel accumulates, due to centrifugal forces caused by rotations of the cartridge, ensuring reliable and efficient wetting. This enhances the detachment of the skin sample from the adhesive element.

[0130] The cartridge body can include a liquid or gel for detaching the skin sample from the adhesive element upon contact with the skin sample and the adhesive element. This can provide an automated and efficient release of the skin sample into the cartridge body, eliminating the need for manual intervention.

[0131] The cartridge can be configured such that rotations of the cartridge, while the lid is in the closed position, cause the liquid or gel to contact the underside of the cartridge lid. This can allow an automated detachment of the skin sample from the adhesive element.

[0132] The liquid or gel can be the receiving medium. As discussed, the receiving medium may enhance sample preservation, maintain sample integrity, and facilitate subsequent processing or analysis.

[0133] The cartridge can be configured to receive the skin sample by providing the skin sample from the collection device to the adhesive element, while the cartridge lid is in the open position and by transitioning the cartridge lid from the open position to the closed position. In particular, while the cartridge lid is in the open position the underside of the cartridge lid can be easily accessible. This can make it easier to provide the skin sample on the adhesive element provided thereon. Upon transitioning the cartridge lid to the closed position, the underside surface faces towards the cartridge body. This way the skin sample can be provided inside the cartridge body. A liquid or gel (e.g., the receiving medium) provided inside the cartridge body may be used to detach the skin sample from the adhesive surface. The detachment may be enhanced by rotations of the cartridge.

[0134] In a preferred embodiment, the cartridge lid can be configured with the adhesive element and the collection device can comprise a spoon-like shape and / or can be configure with the concave section.

[0135] In general, the cartridge can comprise an adhesive element. The cartridge can be configured to receive the skin sample adhered to the adhesive element from the collection device.

[0136] As discussed above, the adhesive element may be provided on the cartridge lid, preferably on an underside surface of the cartridge lid.

[0137] Alternatively or additionally, the adhesive element can be provided on an inner wall of the cartridge body accessible through the cartridge inlet. The skin sample may thus be directly provided from the collection device to the cartridge body, adhered to the adhesive element. The adhesive element can, for example, be provided on a surrounding wall of the cartridge container. For example, the adhesive element may be provided on a bottom surface of the cartridge container. The skin sample may thus be directly provided from the collection device in the cartridge container, adhered to the adhesive element.

[0138] In such embodiments, the cartridge container can be provided inside the cartridge body.

[0139] In a second aspect, the present invention relates to a method for collecting and storing a skin sample of a test subject. The method comprises collecting the skin sample with an engaging segment of a collection device from the test subject and receiving the skin sample in a cartridge after collection.

[0140] The collection device may be a curette.

[0141] Collecting the skin sample may comprise scraping the skin sample from the test subject with the engaging segment.

[0142] The engaging segment may comprise grooves and the method may comprise collecting the skin sample in the grooves.

[0143] The grooves may comprise a depth of at least 1 pm and at most 1000 pm.

[0144] The method may comprise collecting the skin sample from the test subject with an abrasive surface of the engaging segment via abrasion.

[0145] The collection device may be a swab or a brush.

[0146] The engaging segment may comprise a tip which absorbs, collects, or transfers substances from a surface.

[0147] The tip may be made of cotton, foam and / or synthetic fibers, such as, nylon.

[0148] The engaging segment may comprise an adhesive surface.

[0149] The collection device may comprise a handle allowing a user to handle the collection device. The method may comprise the engaging segment contacting, in use, the test subject.

[0150] The engaging segment may comprise an abrasive and / or cutting edge.

[0151] Collecting the skin sample may comprise the engaging segment loosening, such as, separating, the skin sample from the test subject.

[0152] The method may comprise retaining the skin sample on the collection device after collection.

[0153] The skin sample may be retained on the collection device via friction.

[0154] The skin sample may be retained on the collection device adhered to the engaging segment.

[0155] The method may comprise, after collecting the skin sample, dislodging the skin sample from the collection device, e.g., via at last one of: shaking the collection device, wiping the collection device on a surface and washing the collection device in a liquid or gel.

[0156] The method may comprise containing the skin sample in a cartridge container of the cartridge.

[0157] The method may comprise allowing the skin sample enter the cartridge through a cartridge inlet of the cartridge.

[0158] The cartridge may comprise a cartridge lid and wherein the cartridge lid may assume an open position, in which the cartridge lid may allow access to the cartridge inlet and a closed position, in which the cartridge lid blocks access to the cartridge inlet.

[0159] The method may comprise the cartridge inlet allowing access inside a cartridge body of the cartridge.

[0160] The method may comprise performing at least one sample processing step to the skin sample while the skin sample may be inside the cartridge body.

[0161] The method may comprise receiving the skin sample into a transfer kit from the collection device and providing the skin sample from the transfer kit to the cartridge. The method may comprise containing the skin sample in a transfer container of the transfer kit.

[0162] The transfer container may be a test tube.

[0163] Receiving the skin sample into the transfer kit may comprise receiving the skin sample from the collection device into the transfer container.

[0164] The method may comprise receiving the skin sample in the transfer container through a transfer container opening of the transfer container.

[0165] The transfer container may contain a receiving medium and the method may comprise receiving the skin sample within the receiving medium contained in the transfer container to form a sample mixture, said sample mixture comprising the skin sample and the receiving medium.

[0166] The method may comprise receiving a received portion of the collection device in the transfer container such that relative motion between the collection device and the transfer container may be allowed while the received portion is received in the transfer container.

[0167] The method may comprise effecting a relative motion between the transfer container and the collection device, while the received portion is received in the transfer container, to dislodge the skin sample from the collection device.

[0168] The received portion may comprise the engaging segment.

[0169] The method may comprise retaining the skin sample in or on the received portion after collection.

[0170] The received portion may be submerged at least in part in the receiving medium during the relative motion.

[0171] The transfer kit may comprise a transfer cap configured to attach, preferably to releasably attach, to the transfer container.

[0172] The method may comprise covering the transfer container opening with the transfer cap. The transfer cap may comprise a dispensing tip, wherein the dispending tip may comprise a dispensing orifice.

[0173] The dispensing tip may comprise a tip protruding section, wherein the tip protruding section tapers to a tip end such that the cross-sectional area of the dispensing tip decreases towards the tip end.

[0174] The dispensing orifice may be located on the tip end.

[0175] The method may comprise receiving in the cartridge at least a portion of the sample mixture.

[0176] The method may comprise transferring the skin sample from the transfer kit to the cartridge and wherein said transferring may comprise providing, e.g., dripping, at least a portion of the sample mixture from transfer kit into the cartridge container.

[0177] The cartridge container may comprise a hydrophilic coating or an absorbent material that absorbs the sample mixture.

[0178] The method may comprise receiving the sample mixture into the cartridge container via the cartridge inlet.

[0179] Receiving the skin sample into the transfer kit may comprise receiving the skin sample from the collection device into the transfer cap and from the transfer cap into the transfer container.

[0180] The skin sample may be received from the transfer cap into the transfer container during and / or after attaching the transfer cap to the transfer container.

[0181] The cartridge lid may comprise the cartridge container.

[0182] The cartridge container may be integrated in the cartridge lid.

[0183] The method may comprise receiving the skin sample from the collection device into the cartridge container.

[0184] The skin sample may be received from the collection device into the cartridge container through a cartridge container opening of the cartridge container. The cartridge lid may be configured such that in the open position the cartridge container opening faces away from the cartridge inlet and in the closed position the cartridge container opening faces towards the cartridge inlet.

[0185] The cartridge container may be dry while containing the skin sample.

[0186] The cartridge lid may comprise a lid cover and wherein the lid cover may assume an open position, in which the lid cover may allow access to the cartridge container and a closed position, in which the lid cover blocks access to the cartridge container.

[0187] The lid cover may seal the cartridge container in the closed position.

[0188] The lid cover may allow transitions from the closed position to the open position and from the open position to the closed position.

[0189] The lid cover may be elastic.

[0190] The method may comprise transitioning the lid cover from the closed position to the open position and from the open position to the closed position via deformation.

[0191] The lid cover may comprise a foil, such as, an aluminum foil.

[0192] The lid cover may comprise a peripheral section and a central section, wherein the peripheral section may surround the central section and wherein the peripheral section may be configured to contact a peripheral wall of the cartridge container.

[0193] The peripheral section of the lid cover may be configured to attach to the peripheral wall of the cartridge container upon contact.

[0194] A surface of the peripheral section contacting the peripheral wall may comprise an adhesive coating.

[0195] The peripheral wall of the cartridge container may comprise a flat surface that may coincide with the peripheral section of the lid cover when the lid cover may be in the closed position.

[0196] Said flat surface of the peripheral wall may comprise an adhesive coating. The method may comprise receiving the skin sample in the cartridge from the collection device by, optionally, transitioning the lid cover from the closed position to the open position, dislodging the skin sample from the collection device into the cartridge container and transitioning the lid cover from the open position to the closed position.

[0197] The cartridge container may contain a receiving medium and the method may comprise receiving the skin sample within the receiving medium contained in the cartridge container to form a sample mixture, said sample mixture comprising the skin sample and the receiving medium.

[0198] The method may comprise receiving a received portion of the collection device in the cartridge container such that relative motion between the collection device and the cartridge container may be allowed while the received portion may be received in the cartridge container.

[0199] The method may comprise effecting a relative motion between the cartridge container and the collection device, while the received portion may be received in the cartridge container, to dislodge the skin sample from the collection device.

[0200] The received portion may comprise the engaging segment.

[0201] The method may comprise retaining the skin sample in or on the received portion after collection.

[0202] The received portion may be submerged at least in part in the receiving medium during the relative motion.

[0203] The method may comprise receiving the skin sample on a transfer surface from the collection device and the transfer surface supporting the skin sample thereon.

[0204] The method may comprise covering the transfer surface with a surface cover.

[0205] The surface cover may comprise a sample collecting side configured to facilitate adhesion of the skin sample thereon and the method may comprise the skin sample adhering on the sample collecting side. The method may comprise attaching the surface cover and the cartridge lid to each other such that when the cartridge lid, with the surface cover attached thereon, may be in the closed position the surface collecting side faces the cartridge inlet.

[0206] The method may comprise collecting, with a gathering tool of the transfer kit, the skin sample contained in the transfer container.

[0207] The gathering tool may be received, at least in part, in the transfer container such that relative motion between the gathering tool and the transfer container may be allowed while the gathering tool may be received, at least in part, in the transfer container.

[0208] The method may comprise effecting relative motion between the gathering tool and the transfer container, while the gathering tool may be received, at least in part, in the transfer container, to collect, with the gathering tool, the skin sample contained in the transfer container.

[0209] The method may comprise receiving the skin sample in the cartridge from the gathering tool.

[0210] The gathering tool may comprise a gathering section configured to facilitate adhesion of the skin sample to the gathering section.

[0211] The method may comprise removing the gathering section from the gathering tool.

[0212] The gathering section may be at an end of the gathering tool.

[0213] The method may comprise the gathering section dissolving in a liquid or gel, such as, the receiving medium.

[0214] The method may comprise receiving the gathering section in the cartridge.

[0215] The method may comprise dissolving, using the cartridge, the gathering section with the skin sample attached thereto.

[0216] The method may comprise agitating homogenizing particles within the receiving medium to dissect and / or lyse the skin sample received within the receiving medium and homogenize the sample mixture. The method may comprise mechanically agitating the transfer container and / or the cartridge container to agitate the homogenizing particles.

[0217] The method may comprise stirring the sample mixture to agitate the homogenizing particles.

[0218] The method may comprise magnetically agitating at least some of the homogenizing particles.

[0219] The method may comprise using sonication to agitate at least some of the homogenizing particles.

[0220] The method may comprise pressing with a mortar tool the skin sample within the receiving medium to homogenize the sample mixture.

[0221] The receiving medium may comprise reagents for inhibiting skin sample degradation, preferably for inhibiting DNA or RIMA degradation.

[0222] The receiving medium may comprise chaotropic agents.

[0223] The receiving medium may comprise release agents.

[0224] The receiving medium may be a liquid or a gel.

[0225] The method may comprise the cartridge receiving the skin sample from the collection device by dislodging the skin sample from the collection device onto a surface of the cartridge lid facing the cartridge inlet and transitioning the cartridge lid from the open position to the closed position.

[0226] The method may comprise accommodating a metered volume of the sample mixture in a sample compartment of the cartridge container.

[0227] The sample compartment may comprise an absorbent material for absorbing the sample mixture.

[0228] The metered volume may be at least 1 microliter and at most 3 milliliters. The method may comprise the sample mixture exiting the sample compartment via an outlet of the cartridge container.

[0229] The method may comprise accommodating excess sample mixture in an overflow compartment of the cartridge container.

[0230] The method may comprise overflowing of an overflow volume of the sample mixture from the sample compartment and being accommodated thereafter in the overflow compartment.

[0231] The sample compartment and the overflow compartment may be provided adjacent to each other. Moreover, the cartridge container may comprise a metering wall, provided between the sample compartment and the overflow compartment. Further still, a dimension of the metering wall determines the metered volume of the sample mixture.

[0232] The method may comprise flowing of an overflow volume of the sample mixture, over the metering wall, from the sample compartment to the overflow compartment.

[0233] The cartridge may comprise an inlet barrier.

[0234] The inlet barrier may be formed along a perimeter of the cartridge inlet.

[0235] The inlet barrier may prevent a liquid or gel positioned next to the cartridge inlet from entering the cartridge.

[0236] The test subject may be a patient.

[0237] The method may comprise lysing and / or fixating the skin sample with the transfer kit.

[0238] The method may comprise lysing and / or fixating the skin sample with the cartridge.

[0239] The method may comprise lysing and / or fixating the skin sample with the receiving medium.

[0240] The method may comprise, providing with the cartridge, a metered volume of the skin sample and / or of a sample mixture comprising the skin sample to a downstream analysis device for analyzing the skin sample. The method may comprise receiving the cartridge in a downstream analysis device for analyzing the skin sample.

[0241] The method may comprise utilizing the analysis device.

[0242] The method may comprise determining, with the analysis device, a cycle threshold value.

[0243] The method may comprise determining, with the analysis device, an amount of a target gene in the skin sample.

[0244] The method may comprise, determining, with the analysis device, a probability scope for a skin disease.

[0245] The engaging segment can comprise a spoon-like shape.

[0246] The engaging segment can further comprise a sharp edge configured to dislodge the skin sample from the test subject.

[0247] The sharp edge can be circular or elliptical.

[0248] The engaging segment can further comprise a concave section defining a collection space, wherein the concave section can end at the sharp edge and can be configured to collect the skin sample within the collection space.

[0249] The collection space can be accessible through the sharp edge.

[0250] The sharp edge and the concave section can be configured to perform both dislodging and collection of the skin sample in a single scraping action.

[0251] The collection space can be dimensioned to accommodate a predetermined volume of the skin sample.

[0252] The engaging segment can be formed as an integral component of the collection device or can be detachably mounted to a handle.

[0253] In embodiments wherein a cartridge lid is provided, the cartridge lid can comprise an adhesive element and the method can comprise the cartridge lid receiving the skin sample adhered to the adhesive element from the collection device. The cartridge lid can comprise an underside surface that faces towards the cartridge body when the cartridge lid is in the closed position. The adhesive element can be provided on the underside surface, preferably on a peripheral portion of the underside surface.

[0254] The cartridge body can comprise a liquid or a gel. The method can comprise the liquid or the gel detaching the skin sample from the adhesive element upon contact with the skin sample and the adhesive element.

[0255] The method can comprise rotating the cartridge, while the cartridge lid is in the closed position. Rotations of the cartridge can cause the liquid or the gel to contact the underside of the cartridge lid.

[0256] The liquid or gel can be the receiving medium.

[0257] Receiving the skin sample in the cartridge after collection can comprise providing the skin sample from the collection device to the adhesive element, while the cartridge lid is in the open position and transitioning the cartridge lid from the open position to the closed position.

[0258] In a preferred embodiment, the cartridge lid can be configured with the adhesive element and the collection device can comprise a spoon-like shape and / or can be configure with the concave section.

[0259] In general, the cartridge can comprise an adhesive element. The cartridge can be configured to receive the skin sample, adhered to the adhesive element, from the collection device. The method may thus comprise the cartridge receiving the skin sample, adhered to the adhesive element, from the collection device.

[0260] As discussed above, the adhesive element may be provided on the cartridge lid, preferably on an underside surface of the cartridge lid.

[0261] Alternatively or additionally, the adhesive element can be provided on an inner wall of the cartridge body accessible through the cartridge inlet. The method may thus comprise directly providing the skin sample from the collection device to the cartridge body, adhered to the adhesive element. The adhesive element can, for example, be provided on a surrounding wall of the cartridge container. For example, the adhesive element may be provided on a bottom surface of the cartridge container. The method may thus comprise directly providing the skin sample from the collection device in the cartridge container, adhered to the adhesive element.

[0262] In such embodiments, the cartridge container can be provided inside the cartridge body.

[0263] The present invention is also defined by the following numbered embodiments.

[0264] Below, system embodiments will be discussed. These embodiments are abbreviated by the letter "S" followed by a number. When reference is herein made to system embodiments, these embodiments are meant.

[0265] 51. A system for collecting and storing a skin sample (1) of a test subject, the system comprising: a collection device (3) comprising an engaging segment (31), said engaging segment (31) configured to collect the skin sample (1) from the test subject; and a cartridge (5) configured to receive the skin sample (1) after collection.

[0266] 52. The system according to any of the preceding embodiments, wherein the collection device (3) is a curette.

[0267] 53. The system according to any of the preceding embodiments, wherein the engaging segment (31) is configured to collect the skin sample (1) by scraping the skin sample (1) from the test subject.

[0268] 54. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises grooves (312) and wherein the collection device (3) is configured such that the skin sample (1) is collected in the grooves (312).

[0269] 55. The system according to the preceding embodiment, wherein the grooves (312) comprise a depth of at least 1 pm and at most 1000 pm.

[0270] 56. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises an abrasive surface and wherein the abrasive surface is configured to collect the skin sample (1) from the test subject via abrasion. 57. The system according to embodiment SI, wherein the collection device (3) is a swab or a brush.

[0271] 58. The system according to the preceding embodiment, wherein the engaging segment (31) comprises a tip which is configured to absorb, collect, or transfer substances from a surface.

[0272] 59. The system according to any of the preceding embodiments, wherein the tip is made of cotton, foam and / or synthetic fibers, such as, nylon.

[0273] 510. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises an adhesive surface.

[0274] 511. The system according to any of the preceding embodiments, wherein the collection device (3) comprises a handle (33) allowing a user to handle (33) the collection device (3).

[0275] 512. The system according to any of the preceding embodiments, wherein the engaging segment (31) is configured, in use, to contact the test subject.

[0276] 513. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises an abrasive and / or cutting edge.

[0277] 514. The system according to any of the preceding embodiments, wherein the engaging segment (31) is configured to loosen, such as, separate, the skin sample (1) from the test subject.

[0278] 515. The system according to any of the preceding embodiments, wherein the collection device (3) is configured to retain the skin sample (1) after collection.

[0279] 516. The system according to any of the preceding embodiments, wherein the collection device (3) is configured to retain the skin sample (1) via friction.

[0280] 517. The system according to any of the preceding embodiments, wherein the collection device (3) is configured to retain the skin sample (1) adhered to the engaging segment (31). 518. The system according to any of the preceding embodiments, wherein the collection device (3) is configured to allow the skin sample (1) to be dislodged from the collection device (3), e.g., via at last one of: shaking the collection device (3), wiping the collection device (3) on a surface and washing the collection device (3) in a liquid or gel.

[0281] 519. The system according to any of the preceding embodiments, wherein the cartridge (5) comprises a cartridge container (50) configured to contain the skin sample (1).

[0282] 520. The system according to any of the preceding embodiments, wherein the cartridge (5) comprises a cartridge inlet (54) configured to allow the skin sample (1) enter the cartridge (5).

[0283] 521. The system according to the preceding embodiment, wherein the cartridge (5) comprises a cartridge lid (52) and wherein the cartridge lid (52) is configured to assume an open position, in which the cartridge lid (52) is configured to allow access to the cartridge inlet (54) and a closed position, in which the cartridge lid (52) is configured to block access to the cartridge inlet (54).

[0284] 522. The system according to any of the preceding embodiments, wherein the cartridge (5) comprises a cartridge body (53) and wherein the cartridge inlet (54) allows access inside the cartridge body (53).

[0285] 523. The system according to the preceding embodiment, wherein the cartridge (5) is configured to perform at least one sample processing step to the skin sample (1) while the skin sample (1) is inside the cartridge body (53).

[0286] 524. The system according to any of the preceding embodiments, wherein the system further comprises a transfer kit (7) and wherein the transfer kit (7) is configured to receive the skin sample (1) from the collection device (3) and to provide the skin sample (1) to the cartridge (5).

[0287] 525. The system according to the preceding embodiment, wherein the transfer kit (7) comprises a transfer container (70) configured to contain the skin sample (1).

[0288] 526. The system according to the preceding embodiment, wherein the transfer container (70) is a test tube. 527. The system according to any of the 2 preceding embodiments, wherein the transfer kit (7) is configured to receive the skin sample (1) from the collection device (3) into the transfer container (70).

[0289] 528. The system according to any of the 3 preceding embodiments, wherein the transfer container (70) comprises a transfer container opening (72) and wherein the transfer kit (7) is configured to receive the skin sample (1) in the transfer container (70) through the transfer container opening (72).

[0290] 529. The system according to any of the 4 preceding embodiments, wherein the transfer container (70) contains a receiving medium (10) and wherein the transfer kit (7) is configured to receive the skin sample (1) within the receiving medium (10) to form a sample mixture (12), said sample mixture (12) comprising the skin sample (1) and the receiving medium (10).

[0291] 530. The system according to the preceding embodiment, wherein the transfer kit (7) is configured to receive a received portion of the collection device (3) in the transfer container (70) such that relative motion between the collection device (3) and the transfer container (70) is allowed while the received portion is received in the transfer container (70).

[0292] 531. The system according to the preceding embodiment, wherein relative motion between the transfer container (70) and the collection device (3), while the received portion is received in the transfer container (70), causes the skin sample (1) to be dislodged from the collection device (3).

[0293] 532. The system according to any of the 2 preceding embodiments, wherein the received portion comprises the engaging segment (31).

[0294] 533. The system according to any of the 3 preceding embodiments, wherein the skin sample (1) is retained in or on the received portion after collection.

[0295] 534. The system according to any of the 4 preceding embodiments, wherein the received portion is submerged at least in part in the receiving medium (10) during the relative motion.

[0296] 535. The system according to any of the preceding embodiments and with the features of embodiment S25, wherein the transfer kit (7) comprises a transfer cap (74) configured to attach, preferably to releasably attach, to the transfer container (70). 536. The system according to the preceding embodiment and with the features of embodiment S28, wherein the transfer cap (74) is configured to cover the transfer container opening (72).

[0297] 537. The system according to any of the 2 preceding embodiments, wherein the transfer cap (74) comprises a dispensing tip (742), wherein the dispending tip comprises a dispensing orifice (744).

[0298] 538. The system according to the preceding embodiment, wherein the dispensing tip (742) comprises a tip protruding section (746), wherein the tip protruding section (746) tapers to a tip end (748) such that the cross-sectional area of the dispensing tip (742) decreases towards the tip end (748).

[0299] 539. The system according to the 2 preceding embodiments, wherein the dispensing orifice (744) is located on the tip end (748).

[0300] 540. The system according to any of the preceding embodiments and with the features of embodiment S29, wherein the cartridge (5) is configured to receive at least a portion of the sample mixture (12).

[0301] 541. The system according to the preceding embodiment and with the features of embodiment S19, wherein the cartridge (5) and the transfer kit (7) are configured such that the skin sample (1) is transferable from the transfer kit (7) to the cartridge (5) by providing, e.g., by dripping, at least a portion of the sample mixture (12) from transfer kit (7) into the cartridge container (50).

[0302] 542. The system according to the preceding embodiment, wherein the cartridge container (50) comprises a hydrophilic coating or an absorbent material configured to absorb the sample mixture (12).

[0303] 543. The system according to any of the 2 preceding embodiments and with the features of embodiment S20, wherein the cartridge (5) is configured to receive the sample mixture (12) into the cartridge container (50) via the cartridge inlet (54).

[0304] 544. The system according to any of the preceding embodiments and with the features of embodiment S35, wherein the transfer kit (7) is configured to receive the skin sample (1) from the collection device (3) into the transfer cap (74) and from the transfer cap (74) into the transfer container (70).

[0305] 545. The system according to the preceding embodiment, wherein the transfer kit (7) is configured to receive the skin sample (1) from the transfer cap (74) into the transfer container (70) during and / or after attaching the transfer cap (74) to the transfer container (70).

[0306] 546. The system according to any of the preceding embodiments and with the features of embodiment S19 and S21, wherein the cartridge lid (52) comprises the cartridge container (50).

[0307] 547. The system according to the preceding embodiment, wherein the cartridge container (50) is integrated in the cartridge lid (52).

[0308] 548. The system according to any of the 2 preceding embodiments, wherein the cartridge (5) is configured to receive the skin sample (1) from the collection device (3) into the cartridge container (50).

[0309] 549. The system according to any of the 3 preceding embodiments, wherein the cartridge container (50) comprises a cartridge container opening (58) and wherein the cartridge (5) is configured to receive the skin sample (1) from the collection device (3) into the cartridge container (50) through the cartridge container opening (58).

[0310] 550. The system according to the preceding embodiment, wherein the cartridge lid (52) is configured such that in the open position the cartridge container opening (58) faces away from the cartridge inlet (54) and in the closed position the cartridge container opening (58) faces towards the cartridge inlet (54).

[0311] 551. The system according to any of the 5 preceding embodiments, wherein the cartridge container (50) is dry while containing the skin sample (1).

[0312] 552. The system according to any of the preceding embodiments and with the features of embodiment S46, wherein the cartridge lid (52) comprises a lid cover (53) and wherein the lid cover (53) is configured to assume an open position, in which the lid cover (53) is configured to allow access to the cartridge container (50) and a closed position, in which the lid cover (53) is configured to block access to the cartridge container (50).

[0313] 553. The system according to the preceding embodiment, wherein the lid cover (53) is configured to seal the cartridge container (50) in the closed position.

[0314] 554. The system according to any of the 2 preceding embodiments, wherein the lid cover (53) is configured to allow transitions from the closed position to the open position and from the open position to the closed position.

[0315] 555. The system according to any of the 3 preceding embodiments, wherein the lid cover (53) is elastic.

[0316] 556. The system according to the 2 preceding embodiments, wherein the lid cover (53) is configured to be transitioned from the closed position to the open position and from the open position to the closed position via deformation.

[0317] 557. The system according to any of the 5 preceding embodiments, wherein the lid cover (53) comprises a foil, such as, an aluminum foil.

[0318] 558. The system according to any of the 6 preceding embodiments, wherein the lid cover (53) comprises a peripheral section (532) and a central section (534), wherein the peripheral section (532) surrounds the central section (534) and wherein the peripheral section (532) is configured to contact a peripheral wall (502) of the cartridge container (50).

[0319] 559. The system according to the preceding embodiment, wherein the peripheral section (532) of the lid cover (53) is configured to attach to the peripheral wall (502) of the cartridge container (50) upon contact.

[0320] 560. The system according to any of the 2 preceding embodiments, wherein a surface of the peripheral section (532) contacting the peripheral wall (502) comprises an adhesive coating.

[0321] 561. The system according to any of the 3 preceding embodiments, wherein the peripheral wall (502) of the cartridge container (50) comprises a flat surface (503) configured to coincide with the peripheral section (532) of the lid cover (53) when the lid cover (53) is in the closed position. 562. The system according to the preceding embodiment, wherein said flat surface (503) of the peripheral wall (502) comprises an adhesive coating.

[0322] 563. The system according to any of the preceding embodiments and with the features of embodiment S52, wherein the cartridge (5) is configured to receive the skin sample (1) from the collection device (3) by: optionally, transitioning the lid cover (53) from the closed position to the open position; dislodging the skin sample (1) from the collection device (3) into the cartridge container (50); transitioning the lid cover (53) from the open position to the closed position.

[0323] 564. The system according to any of the preceding embodiments, with the features of embodiment S19, and without the features of embodiment S49 wherein the cartridge container (50) contains a receiving medium (10) and wherein the cartridge (5) is configured to receive the skin sample (1) within the receiving medium (10) to form a sample mixture (12), said sample mixture (12) comprising the skin sample (1) and the receiving medium (10).

[0324] 565. The system according to the preceding embodiment, wherein the cartridge (5) is configured to receive a received portion of the collection device (3) in the cartridge container (50) such that relative motion between the collection device (3) and the cartridge container (50) is allowed while the received portion is received in the cartridge container (50).

[0325] 566. The system according to the preceding embodiment, wherein relative motion between the cartridge container (50) and the collection device (3), while the received portion is received in the cartridge container (50), causes the skin sample (1) to be dislodged from the collection device (3).

[0326] 567. The system according to any of the 2 preceding embodiments, wherein the received portion comprises the engaging segment (31).

[0327] 568. The system according to any of the 3 preceding embodiments, wherein the skin sample (1) is retained in or on the received portion after collection. 569. The system according to any of the 4 preceding embodiments, wherein the received portion is submerged at least in part in the receiving medium (10) during the relative motion.

[0328] 570. The system according to any of the preceding embodiments, further comprising a transfer surface (6) configured to receive the skin sample (1) from the collection device (3) and to support the skin sample (1) thereon.

[0329] 571. The system according to the preceding embodiment, further comprising a surface cover (62) configured to cover the transfer surface (6).

[0330] 572. The system according to the preceding embodiment, wherein the surface cover (62) comprises a sample collecting side (64) configured to facilitate adhesion of the skin sample (1) thereon.

[0331] 573. The system according to the preceding embodiment and with the features of embodiment S21, wherein the surface cover (62) and the cartridge lid (52) are configured to attach to each other such that when the cartridge lid (52), with the surface cover (62) attached thereon, is in the closed position the surface collecting side faces the cartridge inlet (54).

[0332] 574. The system according to any of the preceding embodiments and with the features of embodiment S25, wherein the transfer kit (7) comprises a gathering tool (76) configured to collect the skin sample (1) contained in the transfer container (70).

[0333] 575. The system according to the preceding embodiment, wherein the transfer kit (7) is configured such that the gathering tool (76) is receivable, at least in part, in the transfer container (70) such that relative motion between the gathering tool (76) and the transfer container (70) is allowed while the gathering tool (76) is received, at least in part, in the transfer container (70).

[0334] 576. The system according to the preceding embodiment, wherein the transfer kit (7) is configured such that relative motion between the gathering tool (76) and the transfer container (70), while the gathering tool (76) is received, at least in part, in the transfer container (70), allows the gathering tool (76) to collect the skin sample (1) contained in the transfer container (70). 577. The system according to any of the 3 preceding embodiments, wherein the cartridge (5) is configured to receive the skin sample (1) from the gathering tool (76).

[0335] 578. The system according to any of the 4 preceding embodiments, wherein the gathering tool (76) comprises a gathering section (78) configured to facilitate adhesion of the skin sample (1) to the gathering section (78).

[0336] 579. The system according to the preceding embodiment, wherein the gathering section (78) is removable from the gathering tool (76).

[0337] 580. The system according to any of the 2 preceding embodiments, wherein the gathering section (78) is at an end of the gathering tool (76).

[0338] 581. The system according to any of the 3 preceding embodiments, wherein the gathering section (78) is configured to be dissolvable in a liquid or gel, such as, the receiving medium (10).

[0339] 582. The system according to any of the 4 preceding embodiments, wherein the cartridge (5) is configured to receive the gathering section (78).

[0340] 583. The system according to any of the 5 preceding embodiments, wherein the cartridge (5) is configured to dissolve the gathering section (78) with the skin sample (1) attached thereto.

[0341] 584. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, further comprising homogenizing particles (14) within the receiving medium (10) configured to dissect and / or lyse the skin sample (1) received within the receiving medium (10) upon agitation of the homogenizing particles (14) to thereby homogenize the sample mixture (12).

[0342] 585. The system according to the preceding embodiment, wherein the homogenizing particles (14) are agitated via mechanical agitation of the transfer container (70) and / or of the cartridge container (50).

[0343] 586. The system according to any of the 2 preceding embodiments, wherein the homogenizing particles (14) are agitated via stirring of the sample mixture (12). 587. The system according to any of the 3 preceding embodiments, wherein at least some of the homogenizing particles (14) are configured to be magnetically agitated.

[0344] 588. The system according to any of the 4 preceding embodiments, wherein at least some of the homogenizing particles (14) are configured to be agitated via sonication.

[0345] 589. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, wherein the transfer kit (7) comprises a mortar tool (8) configured to press the skin sample (1) within the receiving medium (10) to thereby homogenize the sample mixture (12).

[0346] 590. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, wherein the receiving medium (10) comprises reagents configured to inhibit skin sample (1) degradation, preferably configured to inhibit DNA or RIMA degradation.

[0347] 591. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, wherein the receiving medium (10) comprises chaotropic agents.

[0348] 592. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, wherein the receiving medium (10) comprises release agents.

[0349] 593. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, wherein the receiving medium (10) is a liquid or a gel.

[0350] 594. The system according to any of the preceding embodiments and with the features of embodiment S21, wherein the cartridge (5) is configured to receive the skin sample (1) from the collection device (3) by: dislodging the skin sample (1) from the collection device (3) onto a surface of the cartridge lid (52) facing the cartridge inlet (54); transitioning the cartridge lid (52) from the open position to the closed position.

[0351] 595. The system according to any of the preceding embodiments and with the features of embodiment S19, wherein the cartridge container (50) comprises a sample compartment (504) configured to accommodate a metered volume of a sample mixture (12). 596. The system according to the preceding embodiment, wherein the sample compartment (504) comprises an absorbent material configured to absorb the sample mixture (12).

[0352] 597. The system according to any of the 2 preceding embodiments, wherein the metered volume is at least 1 microliter and at most 3 milliliters.

[0353] 598. The system according to any of the 3 preceding embodiments, wherein the cartridge container (50) comprises an outlet (56) configured to allow the sample mixture (12) exit the sample compartment (504).

[0354] 599. The system according to any of the 4 preceding embodiments, wherein the cartridge container (50) comprises an overflow compartment (506) configured to accommodate excess sample mixture (12).

[0355] 5100. The system according to the preceding embodiment, wherein the overflow compartment (506) is configured to accommodate an overflow volume of the sample mixture (12) that overflows from the sample compartment (504).

[0356] 5101. The system according to any of the 2 preceding embodiments, wherein the sample compartment (504) and the overflow compartment (506) are provided adjacent to each other; wherein the cartridge container (50) comprises a metering wall (508), provided between the sample compartment (504) and the overflow compartment (506); and wherein a dimension of the metering wall (508) determines the metered volume of the sample mixture (12).

[0357] 5102. The system according to any of the preceding embodiments, wherein the cartridge container (50) is configured such that an overflow volume of the sample mixture (12) is allowed to flow over the metering wall (508) from the sample compartment (504) to the overflow compartment (506).

[0358] 5103. The system according to any of the preceding embodiments, wherein the cartridge (5) comprises an inlet barrier (55). 5104. The system according to the preceding embodiment and with the features of embodiment S20, wherein the inlet barrier (55) is formed along a perimeter of the cartridge inlet (54).

[0359] 5105. The system according to any of the 2 preceding embodiments and with the features of embodiment S20, wherein the inlet barrier (55) is configured to prevent liquid or gel positioned next to the cartridge inlet (54) from entering the cartridge (5).

[0360] 5106. The system according to any of the preceding embodiments, wherein the test subject is a patient.

[0361] 5107. The system according to any of the preceding embodiments and with the features of embodiment S24, wherein the transfer kit (7) is configured to lyse and / or fixate the skin sample (1).

[0362] 5108. The system according to any of the preceding embodiments, wherein the cartridge (5) is configured to lyse and / or fixate the skin sample (1).

[0363] 5109. The system according to any of the preceding embodiments and with the features of embodiment S29 and / or S64, wherein the receiving medium (10) is configured to lyse and / or fixate the skin sample (1).

[0364] 5110. The system according to any of the preceding embodiments, wherein the cartridge (5) is configured to provide a metered volume of the skin sample (1) and / or of a sample mixture (12) comprising the skin sample (1) to a downstream analysis device configured to analyze the skin sample (1).

[0365] 5111. The system according to any of the preceding embodiments, wherein the cartridge (5) is configured to be received by a downstream analysis device configured to analyze the skin sample (1).

[0366] SI 12. The system according to any of the 2 preceding embodiments, wherein the system further comprises the analysis device.

[0367] S113. The system according to any of the 3 preceding embodiments, wherein the analysis device is configured to determine a cycle threshold value. 5114. The system according to any of the 4 preceding embodiments, wherein the analysis device is configured to determine an amount of a target gene in the skin sample (1).

[0368] 5115. The system according to any of the 5 preceding embodiments, wherein the analysis device is configured to determine a probability scope for a skin disease.

[0369] 5116. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises a spoon-like shape.

[0370] 5117. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises a sharp edge (314) configured to dislodge the skin sample (1) from the test subject.

[0371] 5118. The system according to the preceding embodiment, wherein the sharp edge (314) is circular or elliptical.

[0372] 5119. The system according to any of the 2 preceding embodiments, wherein the engaging segment (31) further comprises a concave section (318) defining a collection space (316), wherein the concave section (318) ends at the sharp edge (314) and is configured to collect the skin sample (1) in the collection space (316).

[0373] 5120. The system according to the preceding embodiment, wherein the collection space (316) is accessible through the sharp edge (314).

[0374] 5121. The system according to any of the 2 preceding embodiments, wherein the sharp edge (314) and the concave section (318) are configured to perform dislodging and collecting of the skin sample (1) in a single scraping action.

[0375] 5122. The system according to any of the 3 preceding embodiments, wherein the collection space (316) is dimensioned to accommodate a predetermined volume of skin sample (1)

[0376] 5123. The system according to any of the preceding embodiments, wherein the engaging segment (31) is formed as an integral component of the collection device (3) or is detachably mounted to a handle (33) of the collection device (3).

[0377] 5124. The system according to any of the preceding embodiments and with the features of embodiment S21, wherein the cartridge lid (52) comprises an adhesive element (51) and wherein the cartridge lid (52) is configured to receive the skin sample adhered to the adhesive element (51) from the collection device (3).

[0378] 5125. The system according to the preceding embodiment and with the features of embodiment S22, wherein the cartridge lid (52) comprises an underside surface (57) that faces towards the cartridge body (53) when the cartridge lid (52) is in the closed position, wherein the adhesive element (51) is provided on the underside surface (57), preferably on a peripheral portion of the underside surface (57).

[0379] 5126. The system according to any of the 2 preceding embodiments, wherein the cartridge body (53) comprises a liquid or a gel for detaching the skin sample from the adhesive element (51) upon contact with the skin sample and the adhesive element (51).

[0380] 5127. The system according to the preceding embodiment, wherein the cartridge (5) is configured such that rotations of the cartridge, while the cartridge lid (52) is in the closed position, cause the liquid or the gel to contact the underside of the cartridge lid (52).

[0381] 5128. The system according to any of the 2 preceding embodiments, wherein the liquid or gel is the receiving medium.

[0382] 5129. The system according to any of the 5 preceding embodiments, wherein the cartridge (5) is configured to receive the skin sample (1) by providing the skin sample from the collection device (3) to the adhesive element (51), while the cartridge lid (52) is in the open position and by transitioning the cartridge lid (52) from the open position to the closed position.

[0383] 5130. The system according to the preceding embodiment, wherein the collection device (3) comprises the features of any of the embodiments S116 to S122.

[0384] 5131. The system according to any of the preceding embodiments, wherein the cartridge (5) comprises an adhesive element (21) and wherein the cartridge (5) is configured to receive the skin sample (1) adhered to the adhesive element (51) from the collection device (3).

[0385] 5132. The system according to the preceding embodiment and with the features of embodiment S22, wherein the adhesive element (21) is provided on an inner wall of the cartridge body (53) accessible through the cartridge inlet (54). 5133. The system according to any of the 2 preceding embodiments and with the features of embodiment S19, wherein the adhesive element (21) is provided on a surrounding wall of the cartridge container (50).

[0386] 5134. The system according to the preceding embodiment and with the features of embodiment S22, wherein the cartridge container (50) is provided inside the cartridge body (53).

[0387] 5135. The system according to any of the preceding embodiments, wherein the engaging segment (31) comprises a diameter of at least 0.1 mm and at most 2 mm, preferably of at least 0.5 mm and at most 1.5 mm, such as, 1 mm.

[0388] Below, method embodiments will be discussed. These embodiments are abbreviated by the letter "M" followed by a number. When reference is herein made to method embodiments, these embodiments are meant.

[0389] Ml. A method for collecting and storing a skin sample (1) of a test subject, the method comprising: collecting the skin sample with an engaging segment (31) of a collection device (3)

[0390] (1) from the test subject; and receiving the skin sample in a cartridge (5) after collection.

[0391] M2. The method according to any of the preceding method embodiments, wherein the collection device (3) is a curette.

[0392] M3. The method according to any of the preceding method embodiments, wherein collecting the skin sample comprises scraping the skin sample (1) from the test subject with the engaging segment (31).

[0393] M4. The method according to any of the preceding method embodiments, wherein the engaging segment (31) comprises grooves (312) and wherein the method comprises collecting the skin sample (1) in the grooves (312).

[0394] M5. The method according to the preceding embodiment, wherein the grooves (312) comprise a depth of at least 1 pm and at most 1000 pm. M6. The method according to any of the preceding method embodiments, wherein the method comprises collecting the skin sample (1) from the test subject with an abrasive surface of the engaging segment (31) via abrasion.

[0395] M7. The method according to embodiment Ml, wherein the collection device (3) is a swab or a brush.

[0396] M8. The method according to the preceding embodiment, wherein the engaging segment (31) comprises a tip which absorbs, collects, or transfers substances from a surface.

[0397] M9. The method according to any of the preceding method embodiments, wherein the tip is made of cotton, foam and / or synthetic fibers, such as, nylon.

[0398] MIO. The method according to any of the preceding method embodiments, wherein the engaging segment (31) comprises an adhesive surface.

[0399] Mil. The method according to any of the preceding method embodiments, wherein the collection device (3) comprises a handle (33) allowing a user to handle (33) the collection device (3).

[0400] M12. The method according to any of the preceding method embodiments, wherein the method comprises the engaging segment (31) contacting, in use, the test subject.

[0401] M13. The method according to any of the preceding method embodiments, wherein the engaging segment (31) comprises an abrasive and / or cutting edge.

[0402] M14. The method according to any of the preceding method embodiments, wherein collecting the skin sample comprises the engaging segment (31) loosening, such as, separating, the skin sample (1) from the test subject.

[0403] M15. The method according to any of the preceding method embodiments, wherein the method comprises retaining the skin sample (1) on the collection device (3) after collection.

[0404] M16. The method according to any of the preceding method embodiments, wherein the skin sample (1) is retained on the collection device (3) via friction. M17. The method according to any of the preceding method embodiments, wherein the skin sample (1) is retained on the collection device (3) adhered to the engaging segment (31).

[0405] M18. The method according to any of the preceding method embodiments, wherein the method comprises, after collecting the skin sample, dislodging the skin sample (1) from the collection device (3), e.g., via at last one of: shaking the collection device (3), wiping the collection device (3) on a surface and washing the collection device (3) in a liquid or gel.

[0406] M19. The method according to any of the preceding method embodiments, wherein the method comprises containing the skin sample (1) in a cartridge container (50) of the cartridge (5).

[0407] M20. The method according to any of the preceding method embodiments, wherein the method comprises allowing the skin sample (1) enter the cartridge (5) through a cartridge inlet (54) of the cartridge (5).

[0408] M21. The method according to the preceding embodiment, wherein the cartridge (5) comprises a cartridge lid (52) and wherein the cartridge lid (52) assumes an open position, in which the cartridge lid (52) allows access to the cartridge inlet (54) and a closed position, in which the cartridge lid (52) blocks access to the cartridge inlet (54).

[0409] M22. The method according to any of the preceding method embodiments, wherein the method comprises the cartridge inlet (54) allowing access inside a cartridge body (53) of the cartridge (5).

[0410] M23. The method according to the preceding embodiment, wherein the method comprises performing at least one sample processing step to the skin sample (1) while the skin sample (1) is inside the cartridge body (53).

[0411] M24. The method according to any of the preceding method embodiments, wherein the method comprises receiving the skin sample (1) into a transfer kit (7) from the collection device (3) and providing the skin sample (1) from the transfer kit (7) to the cartridge (5). M25. The method according to the preceding embodiment, wherein the method comprises containing the skin sample (1) in a transfer container (70) of the transfer kit (7).

[0412] M26. The method according to the preceding embodiment, wherein the transfer container (70) is a test tube.

[0413] M27. The method according to any of the 2 preceding embodiments, wherein receiving the skin sample (1) into the transfer kit (7) comprises receiving the skin sample (1) from the collection device (3) into the transfer container (70).

[0414] M28. The method according to any of the 3 preceding embodiments, wherein the method comprises receiving the skin sample (1) in the transfer container (70) through a transfer container opening (72) of the transfer container (70).

[0415] M29. The method according to any of the 4 preceding embodiments, wherein the transfer container (70) contains a receiving medium (10) and wherein the method comprises receiving the skin sample (1) within the receiving medium (10) contained in the transfer container (70) to form a sample mixture (12), said sample mixture (12) comprising the skin sample (1) and the receiving medium (10).

[0416] M30. The method according to the preceding embodiment, wherein the method comprises receiving a received portion of the collection device (3) in the transfer container (70) such that relative motion between the collection device (3) and the transfer container (70) is allowed while the received portion is received in the transfer container (70).

[0417] M31. The method according to the preceding embodiment, wherein the method comprises effecting a relative motion between the transfer container (70) and the collection device (3), while the received portion is received in the transfer container (70), to dislodge the skin sample (1) from the collection device (3).

[0418] M32. The method according to any of the 2 preceding embodiments, wherein the received portion comprises the engaging segment (31).

[0419] M33. The method according to any of the 3 preceding embodiments, wherein the method comprises retaining the skin sample (1) in or on the received portion after collection. M34. The method according to any of the 4 preceding embodiments, wherein the received portion is submerged at least in part in the receiving medium (10) during the relative motion.

[0420] M35. The method according to any of the preceding method embodiments and with the features of embodiment M25, wherein the transfer kit (7) comprises a transfer cap (74) configured to attach, preferably to releasably attach, to the transfer container (70).

[0421] M36. The method according to the preceding embodiment and with the features of embodiment M28, wherein the method comprises covering the transfer container opening (72) with the transfer cap (74).

[0422] M37. The method according to any of the 2 preceding embodiments, wherein the transfer cap (74) comprises a dispensing tip (742), wherein the dispending tip comprises a dispensing orifice (744).

[0423] M38. The method according to the preceding embodiment, wherein the dispensing tip (742) comprises a tip protruding section (746), wherein the tip protruding section (746) tapers to a tip end (748) such that the cross-sectional area of the dispensing tip (742) decreases towards the tip end (748).

[0424] M39. The method according to the 2 preceding embodiments, wherein the dispensing orifice (744) is located on the tip end (748).

[0425] M40. The method according to any of the preceding method embodiments and with the features of embodiment M29, wherein the method comprises receiving in the cartridge (5) at least a portion of the sample mixture (12).

[0426] M41. The method according to the preceding embodiment and with the features of embodiment M19, wherein the method comprises transferring the skin sample (1) from the transfer kit (7) to the cartridge (5) and wherein said transferring comprises providing, e.g., dripping, at least a portion of the sample mixture (12) from transfer kit (7) into the cartridge container (50).

[0427] M42. The method according to the preceding embodiment, wherein the cartridge container (50) comprises a hydrophilic coating or an absorbent material that absorbs the sample mixture (12). M43. The method according to any of the 2 preceding embodiments and with the features of embodiment M20, wherein the method comprises receiving the sample mixture (12) into the cartridge container (50) via the cartridge inlet (54).

[0428] M44. The method according to any of the preceding method embodiments and with the features of embodiment M35, wherein receiving the skin sample (1) into the transfer kit (7) comprises receiving the skin sample (1) from the collection device (3) into the transfer cap (74) and from the transfer cap (74) into the transfer container (70).

[0429] M45. The method according to the preceding embodiment, wherein the skin sample (1) is received from the transfer cap (74) into the transfer container (70) during and / or after attaching the transfer cap (74) to the transfer container (70).

[0430] M46. The method according to any of the preceding method embodiments and with the features of embodiment M19 and M21, wherein the cartridge lid (52) comprises the cartridge container (50).

[0431] M47. The method according to the preceding embodiment, wherein the cartridge container (50) is integrated in the cartridge lid (52).

[0432] M48. The method according to any of the 2 preceding embodiments, wherein the method comprises receiving the skin sample (1) from the collection device (3) into the cartridge container (50).

[0433] M49. The method according to any of the 3 preceding embodiments, wherein the skin sample (1) is received from the collection device (3) into the cartridge container (50) through a cartridge container opening (58) of the cartridge container (50).

[0434] M50. The method according to the preceding embodiment, wherein the cartridge lid (52) is configured such that in the open position the cartridge container opening (58) faces away from the cartridge inlet (54) and in the closed position the cartridge container opening (58) faces towards the cartridge inlet (54).

[0435] M51. The method according to any of the 5 preceding embodiments, wherein the cartridge container (50) is dry while containing the skin sample (1). M52. The method according to any of the preceding method embodiments and with the features of embodiment M46, wherein the cartridge lid (52) comprises a lid cover (53) and wherein the lid cover (53) assumes an open position, in which the lid cover (53) allows access to the cartridge container (50) and a closed position, in which the lid cover (53) blocks access to the cartridge container (50).

[0436] M53. The method according to the preceding embodiment, wherein the lid cover (53) seals the cartridge container (50) in the closed position.

[0437] M54. The method according to any of the 2 preceding embodiments, wherein the lid cover (53) allows transitions from the closed position to the open position and from the open position to the closed position.

[0438] M55. The method according to any of the 3 preceding embodiments, wherein the lid cover (53) is elastic.

[0439] M56. The method according to the 2 preceding embodiments, wherein the method comprises transitioning the lid cover (53) from the closed position to the open position and from the open position to the closed position via deformation.

[0440] M57. The method according to any of the 5 preceding embodiments, wherein the lid cover (53) comprises a foil, such as, an aluminum foil.

[0441] M58. The method according to any of the 6 preceding embodiments, wherein the lid cover (53) comprises a peripheral section (532) and a central section (534), wherein the peripheral section (532) surrounds the central section (534) and wherein the peripheral section (532) is configured to contact a peripheral wall (502) of the cartridge container (50).

[0442] M59. The method according to the preceding embodiment, wherein the peripheral section (532) of the lid cover (53) is configured to attach to the peripheral wall (502) of the cartridge container (50) upon contact.

[0443] M60. The method according to any of the 2 preceding embodiments, wherein a surface of the peripheral section (532) contacting the peripheral wall (502) comprises an adhesive coating. M61. The method according to any of the 3 preceding embodiments, wherein the peripheral wall (502) of the cartridge container (50) comprises a flat surface (503) that coincides with the peripheral section (532) of the lid cover (53) when the lid cover (53) is in the closed position.

[0444] M62. The method according to the preceding embodiment, wherein said flat surface (503) of the peripheral wall (502) comprises an adhesive coating.

[0445] M63. The method according to any of the preceding method embodiments and with the features of embodiment M52, wherein the method comprises receiving the skin sample (1) in the cartridge (5) from the collection device (3) by: optionally, transitioning the lid cover (53) from the closed position to the open position; dislodging the skin sample (1) from the collection device (3) into the cartridge container (50); transitioning the lid cover (53) from the open position to the closed position.

[0446] M64. The method according to any of the preceding method embodiments, with the features of embodiment M19, and without the features of embodiment M49, wherein the cartridge container (50) contains a receiving medium (10) and wherein the method comprises receiving the skin sample (1) within the receiving medium (10) contained in the cartridge container (50) to form a sample mixture (12), said sample mixture (12) comprising the skin sample (1) and the receiving medium (10).

[0447] M65. The method according to the preceding embodiment, wherein the method comprises receiving a received portion of the collection device (3) in the cartridge container (50) such that relative motion between the collection device (3) and the cartridge container (50) is allowed while the received portion is received in the cartridge container (50).

[0448] M66. The method according to the preceding embodiment, wherein the method comprises effecting a relative motion between the cartridge container (50) and the collection device (3), while the received portion is received in the cartridge container (50), to dislodge the skin sample (1) from the collection device (3).

[0449] M67. The method according to any of the 2 preceding embodiments, wherein the received portion comprises the engaging segment (31). M68. The method according to any of the 3 preceding embodiments, wherein the method comprises retaining the skin sample (1) in or on the received portion after collection.

[0450] M69. The method according to any of the 4 preceding embodiments, wherein the received portion is submerged at least in part in the receiving medium (10) during the relative motion.

[0451] M70. The method according to any of the preceding method embodiments, wherein the method comprises receiving the skin sample (1) on a transfer surface (6) from the collection device (3) and the transfer surface (6) supporting the skin sample (1) thereon.

[0452] M71. The method according to the preceding embodiment, wherein the method comprises covering the transfer surface (6) with a surface cover (62).

[0453] M72. The method according to the preceding embodiment, wherein the surface cover (62) comprises a sample collecting side (64) configured to facilitate adhesion of the skin sample (1) thereon and wherein the method comprises the skin sample (1) adhering on the sample collecting side (64).

[0454] M73. The method according to the preceding embodiment and with the features of embodiment M21, wherein the method comprises attaching the surface cover (62) and the cartridge lid (52) to each other such that when the cartridge lid (52), with the surface cover (62) attached thereon, is in the closed position the surface collecting side faces the cartridge inlet (54).

[0455] M74. The method according to any of the preceding method embodiments and with the features of embodiment M25, wherein the method comprises collecting, with a gathering tool (76) of the transfer kit (7), the skin sample (1) contained in the transfer container (70).

[0456] M75. The method according to the preceding embodiment, wherein the gathering tool (76) is received, at least in part, in the transfer container (70) such that relative motion between the gathering tool (76) and the transfer container (70) is allowed while the gathering tool (76) is received, at least in part, in the transfer container (70).

[0457] M76. The method according to the preceding embodiment, wherein the method comprises effecting relative motion between the gathering tool (76) and the transfer container (70), while the gathering tool (76) is received, at least in part, in the transfer container (70), to collect, with the gathering tool (76), the skin sample (1) contained in the transfer container (70).

[0458] M77. The method according to any of the 3 preceding embodiments, wherein the method comprises receiving the skin sample (1) in the cartridge (5) from the gathering tool (76).

[0459] M78. The method according to any of the 4 preceding embodiments, wherein the gathering tool (76) comprises a gathering section (78) configured to facilitate adhesion of the skin sample (1) to the gathering section (78).

[0460] M79. The method according to the preceding embodiment, wherein the method comprises removing the gathering section (78) from the gathering tool (76).

[0461] M80. The method according to any of the 2 preceding embodiments, wherein the gathering section (78) is at an end of the gathering tool (76).

[0462] M81. The method according to any of the 3 preceding embodiments, wherein the method comprises the gathering section (78) dissolving in a liquid or gel, such as, the receiving medium (10).

[0463] M82. The method according to any of the 4 preceding embodiments, wherein the method comprises receiving the gathering section (78) in the cartridge (5).

[0464] M83. The method according to any of the 5 preceding embodiments, wherein the method comprises dissolving, using the cartridge, the gathering section (78) with the skin sample (1) attached thereto.

[0465] M84. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the method comprises agitating homogenizing particles (14) within the receiving medium to dissect and / or lyse the skin sample (1) received within the receiving medium (10) and homogenize the sample mixture (12).

[0466] M85. The method according to the preceding embodiment, wherein the method comprises mechanically agitating the transfer container (70) and / or the cartridge container (50) to agitate the homogenizing particles (14). M86. The method according to any of the 2 preceding embodiments, wherein the method comprises stirring the sample mixture (12) to agitate the homogenizing particles (14).

[0467] M87. The method according to any of the 3 preceding embodiments, wherein the method comprises magnetically agitating at least some of the homogenizing particles (14).

[0468] M88. The method according to any of the 4 preceding embodiments, wherein the method comprises using sonication to agitate at least some of the homogenizing particles (14).

[0469] M89. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the method comprises pressing with a mortar tool (8) the skin sample (1) within the receiving medium (10) to homogenize the sample mixture (12).

[0470] M90. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the receiving medium (10) comprises reagents for inhibiting skin sample (1) degradation, preferably for inhibiting DNA or RIMA degradation.

[0471] M91. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the receiving medium (10) comprises chaotropic agents.

[0472] M92. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the receiving medium (10) comprises release agents.

[0473] M93. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the receiving medium (10) is a liquid or a gel.

[0474] M94. The method according to any of the preceding method embodiments and with the features of embodiment M21, wherein the method comprises the cartridge (5) receiving the skin sample (1) from the collection device (3) by: dislodging the skin sample (1) from the collection device (3) onto a surface of the cartridge lid (52) facing the cartridge inlet (54); transitioning the cartridge lid (52) from the open position to the closed position. M95. The method according to any of the preceding embodiments and with the features of embodiment M19, wherein the method comprises accommodating a metered volume of a sample mixture (12) in a sample compartment (504) of the cartridge container (50).

[0475] M96. The method according to the preceding embodiment, wherein the sample compartment (504) comprises an absorbent material for absorbing the sample mixture (12).

[0476] M97. The method according to any of the 2 preceding embodiments, wherein the metered volume is at least 1 microliter and at most 3 milliliters.

[0477] M98. The method according to any of the 3 preceding embodiments, wherein the method comprises the sample mixture (12) exiting the sample compartment (504) via an outlet (56) of the cartridge container (50).

[0478] M99. The method according to any of the 4 preceding embodiments, wherein the method comprises accommodating excess sample mixture (12) in an overflow compartment (506) of the cartridge container (50).

[0479] M100. The method according to the preceding embodiment, wherein the method comprises overflowing of an overflow volume of the sample mixture (12) from the sample compartment (504) and being accommodated thereafter in the overflow compartment (506).

[0480] M101. The method according to any of the 2 preceding embodiments, wherein the sample compartment (504) and the overflow compartment (506) are provided adjacent to each other; wherein the cartridge container (50) comprises a metering wall (508), provided between the sample compartment (504) and the overflow compartment (506); and wherein a dimension of the metering wall (508) determines the metered volume of the sample mixture (12).

[0481] M102. The method according to any of the preceding method embodiments, wherein the method comprises flowing of an overflow volume of the sample mixture (12), over the metering wall (508), from the sample compartment (504) to the overflow compartment (506). M103. The method according to any of the preceding method embodiments, wherein the cartridge (5) comprises an inlet barrier (55).

[0482] M104. The method according to the preceding embodiment and with the features of embodiment M20, wherein the inlet barrier (55) is formed along a perimeter of the cartridge inlet (54).

[0483] M105. The method according to any of the 2 preceding embodiments and with the features of embodiment M20, wherein the inlet barrier (55) prevents liquid or gel positioned next to the cartridge inlet (54) from entering the cartridge (5).

[0484] M106. The method according to any of the preceding method embodiments, wherein the test subject is a patient.

[0485] M107. The method according to any of the preceding method embodiments and with the features of embodiment M24, wherein the method comprises lysing and / or fixating the skin sample (1) with the transfer kit (7).

[0486] M108. The method according to any of the preceding method embodiments, wherein the method comprises lysing and / or fixating the skin sample (1) with the cartridge (5).

[0487] M109. The method according to any of the preceding method embodiments and with the features of embodiment M29 and / or M64, wherein the method comprises lysing and / or fixating the skin sample (1) with the receiving medium (10).

[0488] MHO. The method according to any of the preceding method embodiments, wherein the method comprises, providing with the cartridge (5), a metered volume of the skin sample (1) and / or of a sample mixture (12) comprising the skin sample (1) to a downstream analysis device for analyzing the skin sample (1).

[0489] Mill. The method according to any of the preceding method embodiments, wherein the method comprises receiving the cartridge (5) in a downstream analysis device for analyzing the skin sample (1).

[0490] M112. The method according to any of the 2 preceding embodiments, wherein the method comprises utilizing the analysis device. M113. The method according to any of the 3 preceding embodiments, wherein the method comprises determining, with the analysis device, a cycle threshold value.

[0491] M114. The method according to any of the 4 preceding embodiments, wherein the method comprises determining, with the analysis device, an amount of a target gene in the skin sample (1).

[0492] M115. The method according to any of the 5 preceding embodiments, wherein the method comprises, determining, with the analysis device, a probability score for a skin disease.

[0493] M116. The method according to any of the preceding method embodiments, wherein the engaging segment (31) comprises a spoon-like shape.

[0494] M117. The method according to any of the preceding method embodiments, wherein the engaging segment (31) comprises a sharp edge (314) configured to dislodge the skin sample (1) from the test subject.

[0495] M118. The method according to the preceding embodiment, wherein the sharp edge (314) is circular or elliptical.

[0496] M119. The method according to any of the 2 preceding embodiments, wherein the engaging segment (31) further comprises a concave section (318) defining a collection space (316), wherein the concave section (318) ends at the sharp edge (314) and is configured to collect the skin sample (1) in the collection space (316).

[0497] M120. The method according to the preceding embodiment, wherein the collection space (316) is accessible through the sharp edge (314).

[0498] M121. The method according to any of the 2 preceding embodiments, wherein the sharp edge (314) and the concave section (318) are configured to perform dislodging and collecting of the skin sample (1) in a single scraping action.

[0499] M122. The method according to any of the 3 preceding embodiments, wherein the collection space (316) is dimensioned to accommodate a predetermined volume of skin sample (1) M123. The method according to any of the preceding method embodiments, wherein the engaging segment (31) is formed as an integral component of the collection device (3) or is detachably mounted to a handle (33) of the collection device (3).

[0500] M124. The method according to any of the preceding method embodiments and with the features of embodiment M21, wherein the cartridge lid (52) comprises an adhesive element (51) and wherein the method comprises the cartridge lid (52) receiving the skin sample adhered to the adhesive element (51) from the collection device (3).

[0501] M125. The method according to the preceding embodiment and with the features of embodiment M22, wherein the cartridge lid (52) comprises an underside surface (57) that faces towards the cartridge body (53) when the cartridge lid (52) is in the closed position, wherein the adhesive element (51) is provided on the underside surface (57), preferably on a peripheral portion of the underside surface (57).

[0502] M126. The method according to any of the 2 preceding embodiments, wherein the cartridge body (53) comprises a liquid or a gel and wherein the method comprises the liquid or the gel detaching the skin sample from the adhesive element (51) upon contact with the skin sample and the adhesive element (51).

[0503] M127. The method according to the preceding embodiment, wherein the method comprises rotating the cartridge (5), while the cartridge lid (52) is in the closed position, wherein rotations of the cartridge (5) cause the liquid or the gel to contact the underside of the cartridge lid (52).

[0504] M128. The method according to any of the 2 preceding embodiments, wherein the liquid or gel is the receiving medium.

[0505] M129. The method according to any of the 5 preceding embodiments, wherein receiving the skin sample in the cartridge (5) after collection comprises: providing the skin sample from the collection device (3) to the adhesive element (51), while the cartridge lid (52) is in the open position and transitioning the cartridge lid (52) from the open position to the closed position.

[0506] M130. The method according to the preceding embodiment, wherein the collection device (3) comprises the features of any of the embodiments M116 to M122. M131. The method according to any of the preceding method embodiments, wherein the cartridge (5) comprises an adhesive element (21) and wherein the cartridge (5) is configured to receive the skin sample (1) adhered to the adhesive element (51) from the collection device (3).

[0507] M132. The method according to the preceding embodiment and with the features of embodiment M22, wherein the adhesive element (21) is provided on an inner wall of the cartridge body (53) accessible through the cartridge inlet (54).

[0508] M133. The method according to any of the 2 preceding embodiments and with the features of embodiment M19, wherein the adhesive element (21) is provided on a surrounding wall of the cartridge container (50).

[0509] M134. The method according to the preceding embodiment and with the features of embodiment M22, wherein the cartridge container (50) is provided inside the cartridge body (53).

[0510] Below, use embodiments will be discussed. These embodiments are abbreviated by the letter "U" followed by a number. When reference is herein made to use embodiments, these embodiments are meant.

[0511] Ul. Use of the system according to any of the preceding system embodiments, for collecting, storing and / or analyzing a skin sample (1) of a test subject.

[0512] U2. Use of the system according to any of the preceding system embodiments and with the features of embodiment S112, for analyzing a skin sample (1) of a test subject.

[0513] U3. Use of the system according to any of the preceding system embodiments for generating a probability score, for the skin sample, said probability score indicating a probability that the test subject has a disease, such as, a skin disease.

[0514] Brief description of the drawings

[0515] Figs, la and lb illustrate examples of a collection device;

[0516] Fig. 2 illustrates an example of a transfer kit comprising a transfer container and a transfer cap; Fig. 3 illustrates an example of transferring a skin sample from the collection device to a cartridge using the transfer kit; illustrates a detailed view of the cartridge; illustrates an example of transferring a skin sample from the collection device to the cartridge;

[0517] Fig. 6 depicts a cartridge lid comprising a cartridge container;

[0518] Fig. 7 illustrates a cross-sectional view of the cartridge lid;

[0519] Figs. 8-11 illustrate an example of transferring a skin sample from the collection device to the cartridge container;

[0520] Fig. 12 illustrates a transfer surface;

[0521] Figs. 13-17 illustrate an example of transferring a skin sample from the collection device to the cartridge using the transfer surface;

[0522] Fig. 18 illustrates a transfer container comprising skin samples;

[0523] Fig. 19 illustrates a gathering tool comprising a gathering section;

[0524] Fig. 20 illustrates the gathering tool collecting skin samples from the transfer container;

[0525] Figs. 21(a)-(g) illustrate an example of homogenizing and transferring a sample mixture from the transfer container to the cartridge;

[0526] Figs. 22(a)-(d) illustrate an example of transferring a skin sample from the collection device to the cartridge container via the cartridge lid;

[0527] Fig. 23 illustrates another example of the collection device;

[0528] Figs. 24-25 illustrate a cartridge lid with an adhesive element.

[0529] Detailed description of the drawings

[0530] In the following, exemplary embodiments of the invention will be described, referring to the figures. These examples are provided to give further understanding of the invention, without limiting its scope.

[0531] In the following description, a series of features and / or steps are described. The skilled person will appreciate that unless explicitly required and / or unless required by context, the order of features and steps is not critical for the resulting configuration and its effect. Further, it will be apparent to the skilled person that irrespective of the order of features and steps, the presence or absence of time delay between steps can be present between some or all of the described steps.

[0532] Throughout the present disclosure, "skin sample" and "skin samples" can be used interchangeably. That is, the term "skin sample" can be in the singular or in the plural. In other words, "a skin sample" or "the skin sample" may encompass one or multiple "pieces" of skin.

[0533] Moreover, the terms "scrape sample" and "skin sample" are used interchangeably. For the sake of brevity, "scrape sample" and "skin sample" may sometimes be referred to as "sample".

[0534] The present invention generally relates to a process of sampling a scrape sample and inserting it into a cartridge. Said process can be described in the following discrete steps:

[0535] 1. Sampling: A skin sample can be collected from a patient via scraping with a collection device, e.g., a curette.

[0536] 2. Sample transfer: The sample can be transferred into a transport liquid or gel. However, it will be understood that this step is optional.

[0537] 3. Sample fixation: Sample can be lysed and / or fixated, to ensure a homogenous, stable skin sample. This may, e.g., inhibit RIMA degradation.

[0538] 4. Sample insertion: Sample can be inserted into a cartridge.

[0539] 5. Cartridge can be inserted into a processing instrument, which may also be referred to as an analysis device.

[0540] 6. Cartridge side sample handling: Inside the cartridge additional steps may be performed to the sample. The interface for inserting the sample may have a metering, that ensures a correct volume enters a downstream analysis.

[0541] 7. Analysis: Inside the cartridge, the sample can be processed and / or analyzed. This can include:

[0542] • The skin sample may be lysed, if not already lysed externally.

[0543] • The skin sample may optionally be purified.

[0544] • Target molecules in the skin sample may be amplified, e.g., via PCR (Polymerase Chain Reaction), LAMP (Loop-mediated Isothermal Amplification), and RPA (Recombinase Polymerase Amplification).

[0545] • A signal (typically fluorescent light) can be read out via a reader (typically fluorescence sensor) in the instrument.

[0546] • In case of real-time PCR, the instrument analyses the signal to calculate a Ct (cycle threshold) value. The Ct value can be correlated to an amount of a target gene.

[0547] • An analysis based on machine learning algorithms (e.g., neural networks, linear regression and support vector machine) can be used to calculate a probability score for a skin disease, based on these Ct values or raw fluorescence measurements. The described methods can be implemented in different implementations. Exemplary ones will be discussed below, with reference to the drawings.

[0548] Figs, la and lb depict examples of a collection device 3. The collection device 3 comprises an engaging segment 31 configured to collect a skin sample 1 from a test subject, preferably, by scraping it from the test subject.

[0549] The collection device 3 may comprise handle 33 which may be configured to allow a user to handle, preferably ergonomically, the collection device 3. The collection device 3 may optionally comprise a segment cap 35 which may be configured to cover the engaging segment 31. This may protect the engaging segment 31 to thereby allow it to remain sharp.

[0550] As depicted in Fig. lb, the collection device 3 and in particular the engaging segment 3 may comprise grooves 312. The grooves 312 may comprise a depth of 1 - 1000 pm. The skin can be collected at the grooves 312.

[0551] Other implementations of the collection device 3 include the engaging segment 31 comprising an abrasive surface (not depicted) and / or an adhesive surface (not depicted).

[0552] The collection device 3 may also be a swab (not depicted).

[0553] Fig. 2 depicts a transfer kit 7 comprising a transfer container 70 and a transfer cap 74. The transfer kit 7 can be configured to receive the skin sample from the collection device 3 and to provide the skin sample to a cartridge 5 (see Fig. 3). That is, the transfer kit may be configured to facilitate transferring the skin sample from the collection device 3 to the cartridge 5.

[0554] The transfer container 70 may contain a transfer liquid or gel 10 (which may also be referred to as a receiving medium 10). The transfer container 70 may comprise a transfer container opening 72. The latter may be the only opening of the transfer container 70.

[0555] The transfer cap 74 can be configured to close the transfer container 70. In particular, the transfer cap 74 may be configured to attach, preferably in a releasable manner to the transfer container 70 to thereby cover the transfer container opening 72. The transfer cap 74 and the transfer container 70 may comprise interlocking parts. The transfer cap 74 may comprise a dispensing tip 742, wherein the dispensing tip may comprise a dispensing orifice 748. The dispensing tip 742 may thus allow the contents of the transfer container 70 to exit the transfer container 70 via the dispensing orifice 748. The dispensing tip 742 may comprise a tip protruding section 746 which may taper to a tip end 748 such that the cross-sectional area of the dispensing tip 742 can decrease towards the tip end 748. The dispensing orifice may be located on the tip end 748.

[0556] Fig. 3 depicts a system comprising the collection device 3, a cartridge 5 and the transfer kit 7. In addition, Fig. 3 illustrates a process of transferring a skin sample from the collection device 3 to the transfer kit 7 and from the transfer kit 7 to the cartridge 5.

[0557] As depicted, a skin sample 1 may be collected using the collection device 3. The skin sample 1 may adhere to the collection device 3, e.g., via friction. The collection device 3 may be received in the transfer container 70. In particular, a received portion 37 of the collection device 3 may be received in the transfer container 70 through the transfer container opening 72, said received portion comprising the engaging segment 31. Relative motion between the transfer container 70 and the collection device 70 may facilitate dislodging the skin sample 1 from the collection device and into the transfer container 70. Inside the transfer container a sample mixture 12 may thereby be formed containing the skin sample 1 and the receiving medium 10.

[0558] The transfer cap 74 may be attached to the transfer container 70 to thereby cover the transfer container opening 72. The transfer cap 74 may be locked to the transfer container 70, e.g., via a relative rotation therebetween.

[0559] The transfer container 70 with the transfer cap 74 on, may be used to drip the sample mixture 12 onto the cartridge 5.

[0560] The cartridge 5 may comprises a cartridge container 50 for containing the sample mixture received from the transfer container 70. In this example, the cartridge 5 comprises a cartridge body 53 and the cartridge container 50 is provided inside the cartridge body 53.

[0561] However, the cartridge container 50 may be alternatively provide in a cartridge lid 52 (see, e.g., Fig. 6).

[0562] Furthermore, the skin sample 1 may instead be transferred from the collection device 3 onto the transfer cap 74, such that upon attaching the transfer cap 74 to the transfer container 70, the skin sample may be received in the transfer container 70. That is, in such embodiments, inserting the collection device 3 in the transfer container 70 may be optional.

[0563] Fig. 4 depicts a more detailed view of the cartridge 5, and in particular of the cartridge container 50.

[0564] The cartridge 5 may comprise a cartridge inlet 54, which may be configured to allow access to the cartridge container 50. The cartridge 5 may further comprise a cartridge lid 52 configured to open and close to thereby allow and block access to the cartridge inlet 54.

[0565] The cartridge 5 may comprise an inlet barrier 55 which may be formed along a perimeter of the cartridge inlet 52 to prevent liquid or gel positioned next to the cartridge inlet 54 from entering the cartridge 5.

[0566] The cartridge container 50 may further comprise a cartridge outlet 56 for emptying the cartridge container 50.

[0567] The cartridge container 50 may comprise a sample compartment 504 and an overflow compartment 506, which can be positioned adjacent to each other. The cartridge container 50 can comprise a metering wall 508 between the sample compartment 504 and the overflow compartment 506. A dimension of the metering wall 508 can determine the metered volume of the sample mixture 12. An overflow volume of the sample mixture 12 can flow over the metering wall 508 from the sample compartment 504 to the overflow compartment 508.

[0568] Thus, with reference to Figs. 1-4, a process of sampling a scrape sample and inserting it into a cartridge may comprise:

[0569] 1. Sampling: Scraping with a collection device 3, e.g., a curette 3.

[0570] 2. Sample transfer: Wash the skin sample from the collection device 3 in the transfer container 70, which can be a liquid tube 70. The transfer cap 74 can then be put on the liquid tube 70.

[0571] 3. Sample fixation : The liquid tube 70 may include liquid or gel 10 and preferably grinding particles (which may also be referred to as homogenizing particles 14, see Fig. 21). The liquid tube 70 can be shaken, e.g. on vortex mixer to lyse and / or homogenize the skin sample. Liquid or gel 10 can include reagents to inhibit sample degradation, especially DNA or RIMA degradation. Liquid or gel 10 may include chaotropic agents, e.g. Guanidinium thiocyanate. 4. Sample insertion: Sample can be dripped into an inlet hole of the cartridge 5. The inlet can include a void that has a sufficient volume to receive the skin sample 1. Inlet can include a hydrophilic coating or an absorbent material to "suck" sample into cartridge 5, via capillary forces. Excess liquid or gel in tube 70 can be stored for later experiments. This can be advantageous, in the performance evaluation, where the same sample 1 can be analyzed with the cartridge 5 and a reference method.

[0572] 5. Cartridge 5 can be inserted into a processing instrument, also referred to as an analysis device. Cartridge inlet can be closed with a lid. Cartridge 5 can be inserted into instrument.

[0573] 6. Cartridge side sample handling: Cartridge can include an insert. During step 4, above, the sample can be dripped onto the inlet. The inlet can be fabricated as an insert that is included in the cartridge during manufacturing. The inlet can include an absorbent material and / or structures for metering of a defined sample volume. During rotation of the cartridge 5, liquid or gel 10 can be centrifuged out of the absorbent material. Liquid or gel 10 can slowly flow out of an outlet with a small diameter and respective high fluidic resistance. Any excess liquid or gel 10, over a predefined volume of e.g. between 1 pl to 3 ml, can flow over a metering wall 508 into an overflow compartment 506. The position of the metering wall 508, as defined during manufacturing, can define the metered volume. An additional structure 55 on the inlet can prevent liquid or gel that is dripped next to the inlet from entering the analysis.

[0574] 7. Analysis: Inside the cartridge, the sample can be lysed (only if not already lysed externally), purified (optional), targets amplified (almost always the case, e.g. via PCR, LAMP, RPA, etc.), signal (typically fluorescent) is read out via a (fluorescence) reader in the instrument.

[0575] Fig. 5 depicts a system comprising a collection device 3 and cartridge 5, wherein the skin sample 1 can be transferred from the collection device 3 to the cartridge 5 by providing it directly in the cartridge container 50.

[0576] In particular, the cartridge lid 52 may comprise the cartridge container 50. For example, the cartridge container 50 may be integrated in the cartridge lid 52. The skin sample 1 may be dislodged from the collection device 3 and into the cartridge container 50.

[0577] The cartridge lid 52 may be flipped onto the cartridge inlet 54 to thereby provide the skin sample inside the cartridge body 53. Thus, a process of sampling a scrape sample and inserting it into a cartridge may comprise:

[0578] 1. Sampling: Scraping with a collection device 3, e.g., a curette 3.

[0579] 2. Sample transfer: Provide scrapings in a lid.

[0580] 3. Sample fixation: Lid may contain fixation solution, but may preferably be empty.

[0581] 4. Sample insertion : Lid can be flipped onto cartridge inlet and can be used to close the cartridge inlet.

[0582] 5. Cartridge can be inserted into a processing instrument.

[0583] 6. Cartridge side sample handling: Cartridge can include beads (i.e., homogenizing particles) for homogenization via bead beating. The beads can be actuated by a magnet in the cartridge, that is actuated by a changing magnetic field within the device.

[0584] 7. Analysis: Inside the cartridge, the sample is lysed (only if not already lysed externally), purified (optional), targets amplified (almost always the case, e.g. via PCR, LAMP, RPA, etc.), signal (typically fluorescent) is read out via a (fluorescence) reader in the instrument.

[0585] Fig. 6 depicts an exemplary cartridge lid 52 and Fig. 7 depicts a simplified cross-sectional view of the cartridge lid 52. It will be understood that in the cross-sectional view of Fig. 7 only some structures of the cartridge lid 52 are depicted.

[0586] The cartridge lid 52 can comprise the cartridge container 50 and a lid cover 53 for covering the cartridge container 50. The lid cover 53 can comprise a peripheral section 532 and a central section 534. The peripheral section 532 may surround the central section 534. The cartridge container 50 may comprise a peripheral wall 502 that may laterally surround an inner space of the cartridge container 50.

[0587] The peripheral section 532 and the peripheral wall 502 may be configured such that they may be attached to each other, to thereby seal the cartridge container 50. In Figs. 6 and 7, the lid cover is depicted in a closed position. The peripheral wall 502 may comprise a flat surface 503 that may facilitate attachment, e.g., adhesion, between the peripheral section 532 and the peripheral wall 502.

[0588] The cartridge container 50 may contain receiving medium 10 (similarly as discussed above with respect to the transfer container 70).

[0589] Figs. 8 to 9 illustrate a transfer of the skin sample from the collection device 3 into the cartridge container 50 comprised by the cartridge lid 52. As depicted in Fig. 8, the lid cover 53 may be transitioned from the closed positioned to an open position, e.g., via deformation of the lid cover. Said transition may require a detachment of the peripheral section 532 of the lid cover 53 from the peripheral wall 502 and, e.g., in particular from the flat surface 503 of the peripheral wall.

[0590] As illustrated in Fig. 9, the cartridge container opening 58 may be uncovered by transitioning the lid cover 53 to the open position. This may allow the collection device 3 to be received at least in part in the cartridge container 50.

[0591] As illustrated in Fig. 10, the skin sample 1 may be dislodged from the collection device 3 and into the cartridge container 50. Moreover, the collection device 3 may be removed from the cartridge container 50.

[0592] As illustrated in Fig. 11, the lid cover 53 may be transitioned from the open position to the closed position, e.g., via deformation of the lid cover 53. The lid cover 53 in the closed position may preferably seal the cartridge container 50. Thus, the skin sample 1 may be sealed inside the cartridge container 50.

[0593] Fig. 12 illustrates a transfer surface 6 and a surface cover 62 that can facilitate transferring the skin sample 1 from the collection device 3 to the cartridge 5. The surface cover 62 can be configured to collect the skin sample 1 positioned on the transfer surface 6. In particular, the surface cover 62 can comprise a sample collecting side 64 that can be configured to facilitate attachment of the skin sample 1 thereon.

[0594] The transfer surface 6 and the surface cover 64 may be provided such that the surface cover 64 initially covers at least a portion of the transfer surface 6 and such that the sample collecting side 64 faces towards the transfer surface 6.

[0595] As illustrated in Fig. 13 the surface cover 62 may be separated at least in part from the transfer surface 6. For example, the surface cover 62 may be deformed to thereby uncover the transfer surface 6. The skin sample 1 may be provided from the collection device 3 and onto the transfer surface 6.

[0596] As illustrated in Fig. 14, the collection device 3 may be moved away from the transfer surface 6 with the skin sample remaining on the transfer surface 6.

[0597] As illustrated in Fig. 15, the surface cover 62 may be (re-)attached to the transfer surface 6. Thus, the sample collecting side 64 may contact the skin sample. It will be understood that at this step, different surface cover 62 to the one initially provided (see Fig. 13) may be used. That is, the system may comprise a replacement surface cover 62.

[0598] As illustrated in Fig. 16, the cartridge lid 52 may be aligned with the surface cover 62.

[0599] As illustrated in Fig. 17, the cartridge lid 52 and the lid cover 62 may attach to each other and the lid cover 62 with the skin sample 1 may be separated from the transfer surface 6.

[0600] The cartridge lid 52, with the surface cover 62 attached thereon and with the skin sample 1 attached on the surface cover 62 may be transported away from the transfer surface 6 and towards the cartridge 5. The cartridge lid 52 may provide the skin sample 1 onto the cartridge inlet 54.

[0601] Thus, with reference to Figs. 12-17, a process of sampling a scrape sample and inserting it into a cartridge may comprise:

[0602] 1. Sampling: Scraping with a collection device 3, e.g., a curette 3.

[0603] 2. Sample transfer: Collect scrapings in surface.

[0604] 3. Sample fixation: Tape can contain fixation agent.

[0605] 4. Sample insertion: Sample can be collected with a film from surface and transferred onto lid / into cartridge.

[0606] 5. Cartridge can be inserted into a processing instrument.

[0607] 6. Cartridge side sample handling: Cartridge can include beads (i.e., homogenizing particles) for homogenization via bead beating. The beads can be actuated by a magnet in the cartridge, that is actuated by a changing magnetic field within the device.

[0608] 7. Analysis: Inside the cartridge, the sample is lysed (only if not already lysed externally), purified (optional), targets amplified (almost always the case, e.g. via PCR, LAMP, RPA, etc.), signal (typically fluorescent) is read out via a (fluorescence) reader in the instrument.

[0609] Figs. 18 to 20 illustrate the use of a gathering tool 76 with a gathering section 78 for collecting skin samples from the transfer container. That is, the skin sample 1 can be provided from the collection device 3 to the cartridge 5 via the transfer container 70 and the gathering tool 76.

[0610] With reference to Figs. 18 to 20, a process of sampling a scrape sample and inserting it into a cartridge may comprise: 1. Sampling: Scraping with a collection device 3, e.g., a curette 3. Then, the skin samples can be transferred from the curette 3 into the transfer container 70 by tapping / stripping the curette 3 on the edge of the container and scraping the skin sample into it.

[0611] 2. Sample transfer: Using the gathering tool 76 (e.g., tweezers), the gathering section 78 (e.g., an adhesive ball 78) can be rolled back and forth in the transfer container 70. The skin samples 1 may thereby stick to the gathering section 78.

[0612] 3. Sample insertion: The gathering section 78 (e.g., the ball 78) can be transferred into the cartridge inlet 54.

[0613] 4. Cartridge can be inserted into a processing instrument.

[0614] 5. Cartridge side sample handling: The adhesive ball 78 can then dissolved in the cartridge. The skin sample attached to the adhesive ball 78 may be purified.

[0615] Figs. 21(a)-(g) illustrate an example of homogenizing and transferring a sample mixture from the transfer container 70 to the cartridge 5. As illustrated in Fig. 21(a), the transfer container 70 may contain receiving medium 10 and homogenizing particles 14. The homogenizing particles 14 may be configured to dissect the skin sample 1. Further the transfer cap 74 may be provided (in this example hinged to the transfer container 70).

[0616] As illustrated in Fig. 21(b), the collection device 3 may be received in the transfer container 70 to provide the skin sample 1 therein (as explained above). As illustrated in Fig. 21(c) the transfer cap 74 may be closed. This may allow safely shaking the transfer container 70 as illustrated in Fig. 21(d). Shaking the transfer container 70 may mechanically agitate the homogenizing particles 14 which may dissect the skin sample 1, as illustrated in Fig. 21(d).

[0617] Additionally or alternatively, a mortar tool 8 may be used to dissect the skin sample 1. In this case, the homogenizing particles 14 may be optional. That is, either the homogenizing particles 14 or the mortar tool 8 can be used. It will be understood, that both the homogenizing particles 14 and the mortar tool may also be used.

[0618] As illustrated in Fig. 21(f), a sample mixture 12 may be formed which is homogenized. That is, the skin sample can be dissected into small particles which facilitate further processing.

[0619] As illustrated in Fig. 21(g) the sample mixture 12 may be provided to the cartridge 5, e.g., via dripping. Figs. 22(a)-(d) illustrate an example of transferring a skin sample 1 from the collection device 3 to the cartridge container 50 via the cartridge lid 52.

[0620] In particular, as illustrated in Figs. 22(a) and (b) the cartridge lid 52 may be transitioned from the closed position (Fig. 22(a)), to the open position (Fig. 22(b)). The skin sample 1 may be provided from the collection device 3 on a surface of the cartridge lid 52. Said surface of the cartridge lid 52 can be a surface of the cartridge lid 52 that faces the cartridge container 50 when the cartridge lid 52 is in the closed position.

[0621] As illustrated in Fig. 22(c), the collection device 3 may be moved away from the cartridge 5 and the skin sample 1 may remain on the cartridge lid 52.

[0622] As illustrated in Fig. 22(d), the cartridge lid 52 with the skin sample 1 thereon, may be transitioned to the closed position, to thereby provide the skin sample 1 inside the cartridge container 50.

[0623] Fig. 23 illustrates another example of the collection device 3. As depicted the collection device comprises an engaging segment 31. The engaging segment 31 can comprise a spoon-like shape.

[0624] In particular, the engaging segment 31 can comprise a sharp edge 314. It can further comprise a concave section 318, ending at the sharp edge 314. The sharp edge 314 can comprise a round shape, such as, circular or elliptical, with a diameter of at least 0.1 mm and at most 2 mm, preferably of at least 0.5 mm and at most 1.5 mm, such as, 1 mm. The concave section 318 can define a collection space 316 which can be accessed through the sharp edge 314. The concave section 318 can thus be configured to contain skin samples in the collection space 316. The concave section can comprise a depth of at least 0.5 mm and at most 1.5 mm, such as, 1 mm.

[0625] The sharp edge 314 and the concave section 318 can thus allow dislodging and collecting of the skin sample 1 in a single scraping action. To increase the amount of skin sample, multiple scraping actions may be performed, such as, 10 scaping actions.

[0626] The sampling procedure may comprise applying a moderate pressure of approximately 0.5 kg while moving the collection device 3 along a distance of 0.5 to 1 cm. The engaging segment 31 should be held at an angle of approximately 55° to 75° relative to the skin surface and drawn on the skin surface. The sampling motion, i.e., the scraping action, can repeated 10 times to increase the amount of the skin sample collection.

[0627] A successful sampling procedure may result in a visible groove on the skin. The process can be perceptible but remains tolerable for the test subject. A slight burning sensation may occur, along with temporary redness in a radius of approximately 1 cm around the sampled area, which typically subsides within 30 minutes.

[0628] Fig. 24 depicts a cartridge lid 52 comprising an adhesive element 51. The cartridge lid 52 can be configured to receive a skin sample adhered to the adhesive element 51 from the collection device 3. The adhesive element 51 may be provided on an underside surface 57 of the cartridge lid 52.

[0629] Fig. 25 depicts a system comprising a collection device 3, a cartridge lid 52 and a cartridge

[0630] 5.

[0631] In particular, the cartridge lid 52 may comprise the adhesive element 51, as illustrated in Fig. 24. The skin sample 1 may be dislodged from the collection device 3 and into the adhesive element 51.

[0632] The cartridge lid 52 may be flipped onto the cartridge inlet 54, i.e., transitioned to a closed position, to thereby provide the skin sample inside the cartridge body 53.

[0633] Thus, a process of sampling a skin sample and inserting it into a cartridge may comprise:

[0634] 1. Sampling: Scraping with a collection device 3, e.g., the collection device illustrated in Fig. 23.

[0635] 2. Sample transfer: Provide the skin sample 1 on the adhesive element 51, adhered thereto.

[0636] 3. Sample insertion: Cartridge lid 52 can be flipped onto the cartridge inlet 54 and can be used to close the cartridge inlet 54.

[0637] 4. Cartridge 5 can be inserted into a processing instrument.

[0638] 5. Optionally, the cartridge can include a receiving medium for detaching the skin sample from the adhesive surface.

[0639] 6. Cartridge side sample handling (optional): Cartridge can optionally include beads (i.e., homogenizing particles) for homogenization via bead beating. The beads can be actuated by a magnet in the cartridge, that is actuated by a changing magnetic field within the device. 7. Analysis: Inside the cartridge, the sample is lysed (only if not already lysed externally), purified (optional), targets amplified (almost always the case, e.g. via PCR, LAMP, RPA, etc.), signal (typically fluorescent) is read out via a (fluorescence) reader in the instrument.

[0640] It will be understood that the cartridge lid 52 may also be connected to cartridge body 53, e.g., via a hinge mechanism.

[0641] Moreover, it will be understood that the adhesive element may, alternatively or additionally, be provided elsewhere on the cartridge 5. For example, the adhesive element may be provided on an inner surface of the cartridge body 53 accessible via cartridge inlet 54. For example, referring to the cartridge 5 illustrated in Fig. 3, the adhesive element 21 may be provided on a surrounding wall of the cartridge container 50, such as, on a bottom surface of the cartridge container 50.

[0642] While the present invention has been described with reference to particular embodiments, it is to be understood that these embodiments do not limit the scope of the invention, but merely serve to illustrate the invention.

[0643] Whenever a relative term, such as "about", "substantially" or "approximately" is used in this specification, such a term should also be construed to also include the exact term. That is, e.g., "substantially straight" should be construed to also include "(exactly) straight".

[0644] Whenever steps were recited in the above or also in the appended claims, it should be noted that the order in which the steps are recited in this text may be accidental. That is, unless otherwise specified or unless clear to the skilled person, the order in which steps are recited may be accidental. That is, when the present document states, e.g., that a method comprises steps (A) and (B), this does not necessarily mean that step (A) precedes step (B), but it is also possible that step (A) is performed (at least partly) simultaneously with step (B) or that step (B) precedes step (A). Furthermore, when a step (X) is said to precede another step (Z), this does not imply that there is no step between steps (X) and (Z). That is, step (X) preceding step (Z) encompasses the situation that step (X) is performed directly before step (Z), but also the situation that (X) is performed before one or more steps (Yl), ..., followed by step (Z). Corresponding considerations apply when terms like "after" or "before" are used. While in the above, preferred embodiments have been described with reference to the accompanying drawings, the skilled person will understand that these embodiments were provided for illustrative purpose only and should by no means be construed to limit the scope of the present invention, which is defined by the claims.

Claims

Claims1. A system for collecting and storing a skin sample (1) of a test subject, the system comprising: a collection device (3) comprising an engaging segment (31), said engaging segment (31) configured to collect the skin sample (1) from the test subject; and a cartridge (5) configured to receive the skin sample (1) after collection; wherein the engaging segment (31) is configured to collect the skin sample (1) by scraping the skin sample (1) from the test subject; wherein the cartridge (5) comprises a cartridge container (50) configured to contain the skin sample (1).

2. The system according to the preceding claim, wherein the engaging segment (31) comprises grooves (312) and wherein the collection device (3) is configured such that the skin sample (1) is collected in the grooves (312).

3. The system according to any of the preceding claims, wherein the engaging segment (31) comprises a sharp edge (314) configured to dislodge the skin sample (1) from the test subject; wherein the engaging segment (31) further comprises a concave section (318) defining a collection space (316), wherein the concave section (318) ends at the sharp edge (314) and is configured to collect the skin sample (1) in the collection space (316).

4. The system according to any of the preceding claims, wherein the cartridge (5) comprises a cartridge inlet (54) configured to allow the skin sample (1) enter the cartridge (5); wherein the cartridge (5) comprises a cartridge lid (52) and wherein the cartridge lid (52) is configured to assume an open position, in which the cartridge lid (52) is configured to allow access to the cartridge inlet (54) and a closed position, in which the cartridge lid (52) is configured to block access to the cartridge inlet (54).

5. The system according to the preceding claim, wherein the cartridge lid (52) comprises the cartridge container (50); wherein the cartridge lid (52) comprises a lid cover (53) and wherein the lid cover (53) is configured to assume an open position, in which the lid cover (53) is configured to allow access to the cartridge container (50) and a closed position, in which the lid cover (53) is configured to block access to the cartridge container (50);wherein the cartridge (5) is configured to receive the skin sample (1) from the collection device (3) by: optionally, transitioning the lid cover (53) from the closed position to the open position; dislodging the skin sample (1) from the collection device (3) into the cartridge container (50); transitioning the lid cover (53) from the open position to the closed position.

6. The system according to claim 4, wherein the cartridge lid (52) comprises an adhesive element (51) and wherein the cartridge lid (52) is configured to receive the skin sample adhered to the adhesive element (51) from the collection device (3).

7. The system according to any of the preceding claims, wherein the system further comprises a transfer kit (7) and wherein the transfer kit (7) is configured to receive the skin sample (1) from the collection device (3) and to provide the skin sample (1) to the cartridge (5); and wherein the transfer kit (7) comprises a transfer container (70) configured to contain the skin sample (1).

8. The system according to the preceding claim, wherein the transfer kit (7) is configured to receive the skin sample (1) from the collection device (3) into the transfer container (70); wherein the transfer container (70) contains a receiving medium (10) and wherein the transfer kit (7) is configured to receive the skin sample (1) within the receiving medium (10) to form a sample mixture (12), said sample mixture (12) comprising the skin sample (1) and the receiving medium (10); wherein the transfer kit (7) is configured to receive a received portion of the collection device (3) in the transfer container (70) such that relative motion between the collection device (3) and the transfer container (70) is allowed while the received portion is received in the transfer container (70); wherein relative motion between the transfer container (70) and the collection device (3), while the received portion is received in the transfer container (70), causes the skin sample (1) to be dislodged from the collection device (3); wherein the cartridge (5) and the transfer kit (7) are configured such that the skin sample (1) is transferable from the transfer kit (7) to the cartridge (5) by providing, e.g., by dripping, at least a portion of the sample mixture (12) from transfer kit (7) into the cartridge container (50).

9. The system according to any of the 2 preceding claims, wherein the transfer kit (7) comprises a gathering tool (76) configured to collect the skin sample (1) contained in the transfer container (70); wherein the transfer kit (7) is configured such that the gathering tool (76) is receivable, at least in part, in the transfer container (70) such that relative motion between the gathering tool (76) and the transfer container (70) is allowed while the gathering tool (76) is received, at least in part, in the transfer container (70); wherein the transfer kit (7) is configured such that relative motion between the gathering tool (76) and the transfer container (70), while the gathering tool (76) is received, at least in part, in the transfer container (70), allows the gathering tool (76) to collect the skin sample (1) contained in the transfer container (70); wherein the cartridge (5) is configured to receive the skin sample (1) from the gathering tool (76).

10. The system according to any of the preceding claims, wherein the cartridge container (50) comprises a sample compartment (504) configured to accommodate a metered volume of a sample mixture (12); wherein the cartridge container (50) comprises an overflow compartment (506) configured to accommodate excess sample mixture (12); wherein the overflow compartment (506) is configured to accommodate an overflow volume of the sample mixture (12) that overflows from the sample compartment (504).

11. A method for collecting and storing a skin sample (1) of a test subject, the method comprising: collecting the skin sample with an engaging segment (31) of a collection device (3) (1) from the test subject; and receiving the skin sample in a cartridge (5) after collection; wherein collecting the skin sample comprises scraping the skin sample (1) from the test subject with the engaging segment (31); wherein the method comprises containing the skin sample (1) in a cartridge container (50) of the cartridge (5).

12. The method according to the preceding claim, wherein the engaging segment (31) comprises grooves (312) and wherein the method comprises collecting the skin sample (1) in the grooves (312).

13. The method according to any of the 2 preceding claims,wherein the engaging segment (31) comprises a sharp edge (314) configured to dislodge the skin sample (1) from the test subject; and wherein the engaging segment (31) further comprises a concave section (318) defining a collection space (316), wherein the concave section (318) ends at the sharp edge (314) and is configured to collect the skin sample (1) in the collection space (316).

14. The method according to any of the 2 preceding claims, wherein the method comprises allowing the skin sample (1) enter the cartridge (5) through a cartridge inlet (54) of the cartridge (5); wherein the cartridge (5) comprises a cartridge lid (52) and wherein the cartridge lid (52) assumes an open position, in which the cartridge lid (52) allows access to the cartridge inlet (54) and a closed position, in which the cartridge lid (52) blocks access to the cartridge inlet (54).

15. The method according to the preceding claim, wherein the cartridge lid (52) comprises the cartridge container (50); wherein the cartridge lid (52) comprises a lid cover (53) and wherein the lid cover (53) assumes an open position, in which the lid cover (53) allows access to the cartridge container (50) and a closed position, in which the lid cover (53) blocks access to the cartridge container (50); wherein the method comprises receiving the skin sample (1) in the cartridge (5) from the collection device (3) by: optionally, transitioning the lid cover (53) from the closed position to the open position; dislodging the skin sample (1) from the collection device (3) into the cartridge container (50); transitioning the lid cover (53) from the open position to the closed position.

16. The method according to claim 14, wherein the cartridge lid (52) comprises an adhesive element (51) and wherein the method comprises the cartridge lid (52) receiving the skin sample adhered to the adhesive element (51) from the collection device (3).

17. The method according to any of the 3 preceding claims, wherein the method comprises receiving the skin sample (1) into a transfer kit (7) from the collection device (3) and providing the skin sample (1) from the transfer kit (7) to the cartridge (5); andwherein the method comprises containing the skin sample (1) in a transfer container (70) of the transfer kit (7).

18. The method according to the preceding claim, wherein receiving the skin sample (1) into the transfer kit (7) comprises receiving the skin sample (1) from the collection device (3) into the transfer container (70); wherein the transfer container (70) contains a receiving medium (10) and wherein the method comprises receiving the skin sample (1) within the receiving medium (10) contained in the transfer container (70) to form a sample mixture (12), said sample mixture (12) comprising the skin sample (1) and the receiving medium (10); wherein the method comprises receiving a received portion of the collection device (3) in the transfer container (70) such that relative motion between the collection device (3) and the transfer container (70) is allowed while the received portion is received in the transfer container (70); wherein the method comprises effecting a relative motion between the transfer container (70) and the collection device (3), while the received portion is received in the transfer container (70), to dislodge the skin sample (1) from the collection device (3); wherein the method comprises transferring the skin sample (1) from the transfer kit (7) to the cartridge (5) and wherein said transferring comprises providing, e.g., dripping, at least a portion of the sample mixture (12) from transfer kit (7) into the cartridge container (50).

19. The method according to any of the 2 preceding claims, wherein the method comprises collecting, with a gathering tool (76) of the transfer kit (7), the skin sample (1) contained in the transfer container (70); wherein the gathering tool (76) is received, at least in part, in the transfer container (70) such that relative motion between the gathering tool (76) and the transfer container (70) is allowed while the gathering tool (76) is received, at least in part, in the transfer container (70); wherein the method comprises effecting relative motion between the gathering tool (76) and the transfer container (70), while the gathering tool (76) is received, at least in part, in the transfer container (70), to collect, with the gathering tool (76), the skin sample (1) contained in the transfer container (70); wherein the method comprises receiving the skin sample (1) in the cartridge (5) from the gathering tool (76).

20. The method according to any of the 6 preceding claims,wherein the method comprises accommodating a metered volume of a sample mixture (12) in a sample compartment (504) of the cartridge container (50); wherein the method comprises accommodating excess sample mixture (12) in an overflow compartment (506) of the cartridge container (50); wherein the method comprises overflowing of an overflow volume of the sample mixture (12) from the sample compartment (504) and being accommodated thereafter in the overflow compartment (506).

21. Use of the system according to any of the claims 1 to 10, for collecting, storing and / or analyzing a skin sample (1) of a test subject.

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