Composition containing brain protein hydrolysate, preparation method, and use

Abstract

A composition containing a brain protein hydrolysate, a preparation method, and a use. The composition comprises the following raw materials: porcine brain protein hydrolysate, deer brain protein hydrolysate, bovine collagen peptide powder, Gastrodia elata powder, sea buckthorn powder, hawthorn powder, chicken gizzard membrane powder, vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6, and vitamin B12. The composition has anti-inflammatory and antioxidant effects, and can also be used for preparing a medicament for preventing Alzheimer's disease.

Description

A composition containing brain protein hydrolysate, its preparation method and application Technical Field

[0001] This invention relates to the field of composition preparation technology, and more specifically, to a composition, its preparation method, and its application, wherein the composition is preferably a food composition. Background Technology

[0002] Alzheimer's Disease (AD), first described by German physician Alois Alzheimer in 1906, is a serious neurodegenerative disease characterized primarily by memory decline and cognitive impairment. Today, AD is the fourth leading cause of death worldwide, after cardiovascular disease, cancer, and stroke. As of the end of 2021, there were 51.62 million registered patients globally, including 15 million in my country, the highest number in the world. It is projected that by 2050, the global number of patients will reach 139 million. Even more concerning is that as my country gradually enters an aging society, the number of elderly people is rising significantly. As of the end of 2017, my country had 241 million people aged 60 and above, accounting for 17.3% of the total population (according to international standards, a society is considered to be aging when the population aged 60 and above reaches 10% of the total population). It is estimated that by around 2050, the number of elderly people in my country will reach a peak of 487 million, accounting for 34.9% of the total population. This will objectively increase the potential number of people suffering from Alzheimer's disease (AD) in my country.

[0003] Alzheimer's disease (AD) patients exhibit memory loss, impairment in sensory, judgment, thinking, and motor abilities, cognitive impairment, and personality changes. The long course of AD (cases reported ranging from three to five years to twenty or thirty years) makes AD a heavy burden on society and families. According to statistics from the World Health Organization, the global cost of caring for AD patients currently exceeds the combined costs of cardiovascular disease, cancer, and stroke. However, to date, there is no cure for AD because the exact pathogenesis of AD is not fully understood.

[0004] Therefore, at present, the only way to slow the progression of Alzheimer's disease and improve patients' quality of life is through early diagnosis, early detection, and early intervention. According to calculations by relevant authorities, if the age of onset of Alzheimer's disease can be delayed by 10 years through intervention, the number of patients in the next generation will decrease by 90%.

[0005] While current health supplements and medications based on brain protein hydrolysates have some efficacy in improving memory, their limited composition leads to inconsistent effects, long onset times, short durations of action, and a tendency to develop drug tolerance. Increasing the dosage of these supplements or medications prematurely can cause significant side effects. Because brain protein hydrolysates contain stimulating active ingredients, excessive intake can lead to mental excitement or tension, skin itching and rashes, and other stimulating effects such as hyperactivity, tension, insomnia, hyperventilation, high blood pressure, low blood pressure, fatigue, tremors, depression, apathy, numbness, and cold-like symptoms (chills, cough, respiratory infection). Therefore, enhancing the memory-improving effects of brain protein hydrolysates is an urgent problem that needs to be addressed.

[0006] In view of this, a composition and a preparation method are provided to solve the above problems.

[0007] Summary of the Invention Technical issues

[0008] To overcome the aforementioned deficiencies of the prior art, this invention provides a composition and preparation method. The technical problem this invention aims to solve is that while current health products or drugs based on brain protein hydrolysates have some efficacy in improving memory, their limited composition leads to unstable effects, long onset times, short durations of action, and a tendency to develop drug resistance. Furthermore, abruptly increasing the dosage of such health products or drugs can cause significant side effects.

[0009] Technical solution

[0010] The purpose of this invention is to improve memory and avoid side effects by using a combination of deer brain protein hydrolysate, pig brain protein hydrolysate, bovine collagen peptide powder, and gastrodia elata powder. The addition of sea buckthorn reduces damage caused by oxygen free radicals and accelerates the elimination of free radicals in the body. Hawthorn and chicken gizzard improve the digestive function of the user, ensuring full absorption of the nutrients in the composition. Furthermore, the antioxidant effects of vitamin C and vitamin B5, the role of vitamins B1, B6, and B12 in maintaining nervous system health, and the effect of vitamin B3 in accelerating the hydrolysis of carbohydrates, fats, and proteins contribute to the overall formulation's potential for preventing Alzheimer's disease and its promising application prospects.

[0011] To achieve the above objectives, the present invention provides a composition in a first aspect, the composition comprising the following raw materials:

[0012] Deer brain protein hydrolysate 1-20 parts by weight, pig brain protein hydrolysate 10-80 parts by weight, bovine collagen peptide powder 5-25 parts by weight, gastrodia elata powder 40-100 parts by weight, sea buckthorn powder 1000-6000 parts by weight, hawthorn powder 2000-9000 parts by weight, chicken gizzard powder 3000-10000 parts by weight, vitamin C 15-33.75 parts by weight, vitamin B1 0.135-0.33 parts by weight, vitamin B3 1.65-4.95 parts by weight, vitamin B5 0.33-1.2 parts by weight, vitamin B6 0.105-0.33 parts by weight, vitamin B12 0.15×10 -3 -0.99×10 -3 Parts by weight.

[0013] In one embodiment of the first aspect of the present invention, the composition comprises the following raw materials: 1-20 mg / dose of deer brain protein hydrolysate, 10-80 mg / dose of porcine brain protein hydrolysate, 5-25 mg / dose of bovine collagen peptide powder, 40-100 mg / dose of gastrodia elata powder, 1-6 g / dose of sea buckthorn powder, 2-9 g / dose of hawthorn powder, 3-10 g / dose of chicken gizzard powder, 15-33.75 mg / dose of vitamin C, 0.135-0.33 mg / dose of vitamin B1, 1.65-4.95 mg / dose of vitamin B3, 0.33-1.2 mg / dose of vitamin B5, 0.105-0.33 mg / dose of vitamin B6, and 0.15-0.99 μg / dose of vitamin B12.

[0014] In one embodiment of the first aspect of the invention, the composition is a food composition, preferably a food composition for preventing Alzheimer's disease.

[0015] In a second aspect of the invention, a method for preparing the composition of the invention is provided, comprising the following steps:

[0016] S1. Carry out the preparation of animal-derived ingredients, complete the preparation of deer brain protein hydrolysate, pig brain protein hydrolysate, bovine collagen peptide powder and chicken gizzard powder, and mix them according to the proportions described in the first aspect, label them as "animal dry powder", and place them in a dry container for later use;

[0017] S2. Prepare plant-based ingredients, including Gastrodia elata powder, sea buckthorn powder, and hawthorn powder, and mix them according to the proportions described in the first aspect. Label them as "plant powder" and place them in a dry container for later use.

[0018] S3. Mix solid powders of vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12 evenly according to the proportions described in the second aspect, and pass them through an 80-mesh sieve for later use as excipients;

[0019] S4. Mix the "animal powder" prepared in S1 and the "plant powder" prepared in S2, then add the excipients prepared in S3 to the mixture, and complete the preparation of the finished composition by granulation and drying.

[0020] In one embodiment of the second aspect of the present invention, the preparation steps of the deer brain protein hydrolysate, the porcine brain protein hydrolysate, and the bovine collagen peptide powder in S1 are as follows:

[0021] S1. First, remove impurities from fresh deer brain, pig brain or beef bone manually, then crush it into tissue blocks of 2-3 cm using a shredder. After that, wash the crushed tissue blocks with water, put them into a cooking tank and add water for cooking. The volume ratio of tissue blocks to water is 1:3, the cooking temperature is 100℃, the cooking pressure is 2MPa, and the cooking time is 4 hours to obtain the cooking liquid.

[0022] S2. The cooking liquor obtained in S1 is subjected to oil-water separation by gravity separation method, and the resulting aqueous phase is cooled to 50-55℃ to obtain crude collagen solution;

[0023] S3. Add the compound enzyme preparation to the crude collagen solution obtained in S2, mix evenly, and then enzymatically hydrolyze for 1 hour to obtain a collagen peptide solution. The compound enzyme preparation is composed of trypsin, chymotrypsin and alkaline protease.

[0024] S4. The collagen peptide solution obtained in S3 is filtered to remove tissue fragments and other impurities, and then filtered using an inorganic nanofiltration ceramic membrane with a molecular weight cutoff of 800 Da to further obtain purified collagen peptides.

[0025] S5. Concentrate the purified collagen peptides obtained in S4, and spray dry them to obtain the final deer brain protein hydrolysate, pig brain protein hydrolysate, or bovine collagen peptide powder.

[0026] In S1, deer brain is used as raw material to obtain deer brain protein hydrolysate in S5; in S1, pig brain is used as raw material to obtain pig brain protein hydrolysate in S5; and in S1, bovine bone is used as raw material to obtain bovine collagen peptide powder in S5.

[0027] In one embodiment of the second aspect of the present invention, the preparation steps of the chicken gizzard powder in S1 are as follows:

[0028] S1. Prepare fresh chicken gizzard lining, clean it, and then air dry or bake it so that it can be ground into powder later;

[0029] S2. Place the dried or oven-dried chicken gizzard into an iron pot, add an appropriate amount of sea salt, and stir-fry over low heat until the chicken gizzard bubbles and emits a light smoky aroma.

[0030] S3. Place the stir-fried chicken gizzard into a grinder and grind it multiple times until you obtain powder with a particle size of less than 10 micrometers.

[0031] S4. Pack the ground chicken gizzard powder into a clean glass bottle, seal it, and store it for later use.

[0032] In one embodiment of the second aspect of the present invention, the preparation steps of the gastrodia powder, sea buckthorn powder and hawthorn powder in S2 are as follows:

[0033] S1. Select fresh, intact, and pest-free parts of Gastrodia elata root / sea buckthorn fruit / hawthorn fruit;

[0034] S2. After washing the Gastrodia elata root / sea buckthorn fruit / hawthorn fruit with clean water, dry them. The drying method is to air dry or wipe them dry with a paper towel.

[0035] S3. Cut the dried Gastrodia elata root / sea buckthorn fruit / hawthorn fruit into small pieces of 2-3 mm. The cutting is done by a knife or a cutting machine.

[0036] S4. Grind the cut Gastrodia elata root / sea buckthorn fruit / hawthorn fruit into powder;

[0037] S5. Use a fine grinding sieve to screen the ground Gastrodia elata root / sea buckthorn fruit / hawthorn fruit powder to remove larger particles and impurities, and obtain powder with a particle size of less than 10 micrometers;

[0038] S6. Place the powder of Gastrodia elata root / sea buckthorn fruit / hawthorn fruit into a dry, sealed container and store it in a cool, dry place.

[0039] In one embodiment of the second aspect of the invention, the composition is a food composition, preferably a food composition for preventing Alzheimer's disease.

[0040] The third aspect of the invention provides the use of the composition containing brain protein hydrolysate of the first aspect of the invention or the composition containing brain protein hydrolysate prepared by the method of the second aspect of the invention in the preparation of a composition for the prevention of Alzheimer's disease. Beneficial effects

[0041] By using a combination of deer brain protein hydrolysate, pig brain protein hydrolysate, bovine collagen peptide powder, and gastrodia elata powder, this formula enhances memory while avoiding side effects. The addition of sea buckthorn reduces damage caused by free radicals and accelerates the elimination of free radicals in the body. Hawthorn and chicken gizzard improve digestion, ensuring optimal absorption of nutrients. Furthermore, the antioxidant effects of vitamin C and vitamin B5, the role of vitamins B1, B6, and B12 in maintaining nervous system health, and the effect of vitamin B3 in accelerating the hydrolysis of carbohydrates, fats, and proteins contribute to the overall formula's potential for preventing Alzheimer's disease and its promising application prospects. Attached Figure Description

[0042] Figure 1 shows that none of the five formulations of this invention showed toxicity to PC12 cells; on the contrary, they showed certain nutritional effects that promoted growth.

[0043] Figure 2 shows that none of the five formulations of this invention had a significant effect on mouse weight, indicating that they are biosafe.

[0044] Figure 3 shows that the five formulations of this invention did not cause any lesions in the internal organs of mice, further demonstrating that they are biosafe.

[0045] Figure 4 shows that all five formulations of the present invention have a reducing effect on IL-6 in mouse blood, indicating that they have an anti-inflammatory effect.

[0046] Figure 5 shows that the five formulations of this invention all have the effect of reducing ROS in mouse blood, indicating that they have an antioxidant effect. Detailed Implementation

[0047] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0048] Example:

[0049] Formula 1: The ingredients include 1mg / dose of deer brain protein hydrolysate, 20mg / dose of pig brain protein hydrolysate, 5mg / dose of bovine collagen peptide powder, 40mg / dose of gastrodia elata powder, 1g / dose of sea buckthorn powder, 2g / dose of hawthorn powder, 3g / dose of chicken gizzard powder, 15mg / dose of vitamin C, 0.135mg / dose of vitamin B1, 1.65mg / dose of vitamin B3, 0.33mg / dose of vitamin B5, 0.105mg / dose of vitamin B6, and 0.15μg / dose of vitamin B12.

[0050] Formula 2: The ingredients include deer brain protein hydrolysate 20mg / dose, pig brain protein hydrolysate 40mg / dose, bovine collagen peptide powder 20mg / dose, gastrodia elata powder 60mg / dose, sea buckthorn powder 3g / dose, hawthorn powder 6g / dose, chicken gizzard powder 9g / dose, vitamin C 15mg / dose, vitamin B1 0.2mg / dose, vitamin B3 3.5mg / dose, vitamin B5 1.1mg / dose, vitamin B6 0.2mg / dose, and vitamin B12 0.2μg / dose.

[0051] Formula 3: The ingredients include 15mg / dose of deer brain protein hydrolysate, 60mg / dose of pig brain protein hydrolysate, 15mg / dose of bovine collagen peptide powder, 80mg / dose of gastrodia elata powder, 4g / dose of sea buckthorn powder, 7g / dose of hawthorn powder, 8g / dose of chicken gizzard powder, 30mg / dose of vitamin C, 0.3mg / dose of vitamin B1, 4.5mg / dose of vitamin B3, 1mg / dose of vitamin B5, 0.3mg / dose of vitamin B6, and 0.9μg / dose of vitamin B12.

[0052] Formula 4: The ingredients include 18mg / dose of deer brain protein hydrolysate, 70mg / dose of pig brain protein hydrolysate, 20mg / dose of bovine collagen peptide powder, 90mg / dose of gastrodia elata powder, 5g / dose of sea buckthorn powder, 8g / dose of hawthorn powder, 9g / dose of chicken gizzard powder, 30mg / dose of vitamin C, 0.3mg / dose of vitamin B1, 4.5mg / dose of vitamin B3, 1mg / dose of vitamin B5, 0.3mg / dose of vitamin B6, and 0.9μg / dose of vitamin B12.

[0053] Formula 5: The ingredients include deer brain protein hydrolysate 20mg / dose, pig brain protein hydrolysate 80mg / dose, bovine collagen peptide powder 25mg / dose, gastrodia elata powder 100mg / dose, sea buckthorn powder 6g / dose, hawthorn powder 9g / dose, chicken gizzard powder 10g / dose, vitamin C 33.75mg / dose, vitamin B1 0.33mg / dose, vitamin B3 4.95mg / dose, vitamin B5 1.2mg / dose, vitamin B6 0.33mg / dose, and vitamin B12 0.99μg / dose.

[0054] Preparation method:

[0055] Solid powders of vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6, and vitamin B12 are mixed evenly and passed through an 80-mesh sieve to form excipients. Subsequently, deer brain protein hydrolysate, pig brain protein hydrolysate, bovine collagen peptide powder, chicken gizzard powder, gastrodia elata powder, sea buckthorn powder, and hawthorn powder are added to the excipients. The finished product composition is then prepared by granulation and drying.

[0056] Experimental Example

[0057] The compositions prepared using the five formulations described above were tested:

[0058] Example 1: PC12 cells (rat adrenal medullary pheochromocytoma differentiated cell line) are a type of nerve cell frequently used for studying biotoxicity. In this example, the MTT assay was used to analyze the neurotoxicity of five formulated products on PC12 cells. As shown in Figure 1, compared with the group without formulated products (blank solvent), none of the five formulated products showed toxicity to nerve cells; on the contrary, they showed a certain nutritional effect that promoted growth.

[0059] Experiment Example 2: Sixty mice were randomly divided into six groups of ten each. The control group and the five formulations were fed to the mice twice daily for 14 days to monitor changes in mouse weight and determine the product's toxicity to the organism. As shown in Figure 2, compared to the group without the formulation, none of the five formulations showed a significant effect on mouse weight, indicating that the products are biosafe.

[0060] Example 3: In this example, the mice fed in Example 2 were euthanized in accordance with experimental ethics. Their hearts, livers, spleens, lungs, and kidneys were sectioned using hematoxylin and eosin (HE) and observed under an optical microscope. As shown in Figure 3, the results showed that the five formulations did not cause any lesions in the mice's internal organs, further demonstrating that the products are biosafe.

[0061] Experiment Example 4: In this experiment, mice that had finished feeding in Experiment Example 2 were euthanized in accordance with experimental ethics, and the concentration of the inflammatory cytokine interleukin-6 (IL-6) mRNA in their blood was measured. As shown in Figure 4, the results showed that compared with the control, all five formulations reduced IL-6 in the blood of mice, indicating that the above products have anti-inflammatory effects.

[0062] Example 5: In this example, the mice from Example 2 that had finished feeding were euthanized in accordance with experimental ethics, and the concentration of reactive oxygen species (ROS) in their blood was measured. As shown in Figure 5, the results showed that, compared with the control, all five formulations reduced ROS in the mouse blood, indicating that the above products have antioxidant effects.

[0063] Based on Experiments 1 to 5, it can be confirmed that the composition of the present invention is non-toxic to the test subjects and exhibits a certain growth-promoting nutritional effect on PC12 cells, which represent nerve cells; it can reduce IL-6 in the blood, indicating an anti-inflammatory effect; and it has a reducing effect on ROS in the blood, indicating an antioxidant effect.

Claims

1. A composition characterized in that, It contains the following raw materials in the following proportions: Deer brain protein hydrolysate 1-20 parts by weight, pig brain protein hydrolysate 10-80 parts by weight, bovine collagen peptide powder 5-25 parts by weight, gastrodia elata powder 40-100 parts by weight, sea buckthorn powder 1000-6000 parts by weight, hawthorn powder 2000-9000 parts by weight, chicken gizzard powder 3000-10000 parts by weight, vitamin C 15-33.75 parts by weight, vitamin B1 0.135-0.33 parts by weight, vitamin B3 1.65-4.95 parts by weight, vitamin B5 0.33-1.2 parts by weight, vitamin B6 0.105-0.33 parts by weight, vitamin B12 0.15×10 -3 -0.99×10 -3 Parts by weight.

2. The composition of claim 1, wherein, The composition is a food composition.

3. A process for preparing the composition of claim 1, characterized in that, Includes the following steps: S1. Prepare animal-derived ingredients, including deer brain protein hydrolysate, pig brain protein hydrolysate, bovine collagen peptide powder and chicken gizzard powder, mix them according to the proportions described in claim 1, label them as "animal dry powder", and place them in a dry container for later use; S2. Prepare plant-derived ingredients, complete the preparation of gastrodia elata powder, sea buckthorn powder, and hawthorn powder, mix them according to the proportions described in claim 1, label them as "plant powder", and place them in a dry container for later use; S3. Mix solid powders of vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12 evenly according to the proportions specified in claim 1, and pass them through an 80-mesh sieve for later use as excipients; S4. Mix the "animal powder" prepared in S1 and the "plant powder" prepared in S2, then add the excipients prepared in S3 to the mixture, and complete the preparation of the finished composition by granulation and drying.

4. The method of claim 3, wherein, The preparation steps of the deer brain protein hydrolysate, porcine brain protein hydrolysate, and bovine collagen peptide powder in S1 are as follows: S1. First, remove impurities from fresh deer brain, pig brain or beef bone manually, then crush it into tissue blocks of 2-3 cm using a shredder. After that, wash the crushed tissue blocks with water, put them into a cooking tank and add water for cooking. The volume ratio of tissue blocks to water is 1:3, the cooking temperature is 100℃, the cooking pressure is 2MPa, and the cooking time is 4 hours to obtain the cooking liquid. S2. The cooking liquor obtained in S1 is subjected to oil-water separation by gravity separation method, and the resulting aqueous phase is cooled to 50-55℃ to obtain crude collagen solution; S3. Add the compound enzyme preparation to the crude collagen solution obtained in S2, mix evenly, and then enzymatically hydrolyze for 1 hour to obtain a collagen peptide solution. The compound enzyme preparation is composed of trypsin, chymotrypsin and alkaline protease. S4. The collagen peptide solution obtained in S3 is filtered to remove tissue fragments and other impurities, and then filtered using an inorganic nanofiltration ceramic membrane with a molecular weight cutoff of 800 Da to further obtain purified collagen peptides. S5. Concentrate the purified collagen peptides obtained in S4, and spray dry them to obtain the final deer brain protein hydrolysate, pig brain protein hydrolysate, or bovine collagen peptide powder. In S1, deer brain is used as raw material to obtain deer brain protein hydrolysate in S5; in S1, pig brain is used as raw material to obtain pig brain protein hydrolysate in S5; and in S1, bovine bone is used as raw material to obtain bovine collagen peptide powder in S5.

5. The method of claim 3, wherein, The preparation steps of the chicken gizzard powder in S1 are as follows: S1. Prepare fresh chicken gizzard lining, clean it, and then air dry or bake it so that it can be ground into powder later; S2. Place the dried or oven-dried chicken gizzard into an iron pot, add an appropriate amount of sea salt, and stir-fry over low heat until the chicken gizzard bubbles and emits a light smoky aroma. S3. Place the stir-fried chicken gizzard into a grinder and grind it multiple times until you obtain powder with a particle size of less than 10 micrometers. S4. Pack the ground chicken gizzard powder into a clean glass bottle, seal it, and store it for later use.

6. The method according to claim 3, characterized by The preparation steps of Gastrodia elata powder, Hippophae rhamnoides powder and hawthorn powder in S2 are as follows: S1. Select fresh, intact, and pest-free parts of Gastrodia elata root / sea buckthorn fruit / hawthorn fruit; S2. After washing the Gastrodia elata root / sea buckthorn fruit / hawthorn fruit with clean water, dry them. The drying method is to air dry or wipe them dry with a paper towel. S3. Cut the dried Gastrodia elata root / sea buckthorn fruit / hawthorn fruit into small pieces of 2-3 mm. The cutting is done by a knife or a cutting machine. S4. Grind the cut Gastrodia elata root / sea buckthorn fruit / hawthorn fruit into powder; S5. Use a fine grinding sieve to screen the ground Gastrodia elata root / sea buckthorn fruit / hawthorn fruit powder to remove larger particles and impurities, and obtain powder with a particle size of less than 10 micrometers; S6. Place the powdered Gastrodia elata root / sea buckthorn fruit / hawthorn fruit into a dry, airtight container and store it in a cool, dry place. Place.

7. The method of any one of claims 3 to 6, wherein, The composition is a food composition.

8. Use of the composition of claim 1 or the composition prepared by any one of claims 3 to 6 in the preparation of a medicament for the prevention of Alzheimer's disease.

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