Fully automatic multifunctional pilot platform system and chromatographic system thereof

Abstract

Provided in the present invention are a fully automatic multifunctional pilot platform system and a chromatographic system thereof. The platform system comprises a first input main pipe and a second input main pipe; the first input main pipe is connected to a plurality of input pipes, and is controlled to be turned on and off by means of corresponding valves. The second input main pipe is also connected to said plurality of input pipes, and is controlled to be turned on or off by corresponding valves. Outlet ends of the plurality of input pipes are respectively connected to corresponding liquid inlet ports of a movable mounting module. The movable mounting module is further provided with a plurality of return ports and a plurality of liquid outlet ports. The liquid outlet ports are respectively connected to a plurality of collection pipes by means of output pipes. In the chromatographic system, an RTF column is installed in the movable mounting module. When in use, two tangential flow chromatography columns are used and operate alternately: one is in a loading mode, and the other is in an elution mode. The present invention has the advantages of modularity, achieving a plurality of functions for different process requirements, and satisfying the needs of biopharmaceutical downstream processing workflows.

Description

Fully automated multifunctional pilot platform system and its chromatographic system Technical Field

[0001] This utility model relates to downstream platforms in biomedicine, and in particular to a fully automated multifunctional pilot platform system and its chromatographic system. Background Technology

[0002] Downstream processes in the biopharmaceutical industry include filtration, primarily used to remove impurities such as cell debris, bacteria, and viruses. Filtration includes NFF and TFF. Purification, mainly used for the separation, purification, and refining of target products, involves the use of chromatography columns.

[0003] Existing technologies, such as patent number 202120067489.X, entitled "A Hydraulically Driven Dynamic Chromatography Device," involve injecting liquid into the column through the inlet and collecting it through the outlet when liquid needs to be added for separation. Alternatively, patent number 201820028656.8, entitled "A Moving Beam Type Fully Automatic Chromatography Column," uses a bottom structure consisting of a sieve plate, a flow divider, and a base. A lower nozzle is positioned at the center of the sieve plate. When liquid needs to be added for separation, it enters through the chromatography port of the upper nozzle and exits through the chromatography port of the lower nozzle, or vice versa. The disadvantages are: 1. It cannot achieve upper column flushing or elution; 2. It does not support reverse flushing of the filter plate (sieve plate), easily leading to clogging; 3. It cannot simultaneously achieve high linear flow rate and overload loading, resulting in low dynamic loading capacity. The applicant has proposed a tangential flow chromatography column, patent number 202220697921.8, entitled "A Chromatographic System for Radial and Tangential Flow Bottom of a Chromatographic Column and its Combined Use." Employing a tangential flow structure, it achieves tangential flow filtration while simultaneously loading chromatographic samples, thereby realizing high linear flow rates and overload loading, achieving extremely high dynamic loading capacity.

[0004] Therefore, downstream technologies in biomedicine require different platforms to implement different processes, necessitating the combination of various systems. The drawbacks are: the platform types are numerous and limited; a single platform can only meet the needs of a specific process and cannot be shared. This invention modularizes the platform to achieve different processes, thereby reducing costs.

[0005] Utility Model Content

[0006] To overcome the shortcomings of the prior art, this utility model provides a fully automated multifunctional pilot platform system and its chromatographic system, which can be modularized and multifunctional to meet different process requirements, satisfy the production processes of downstream biopharmaceuticals, and achieve the effect of cost reduction and efficiency improvement.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0008] To address the aforementioned technical problems, the first aspect of this utility model discloses a fully automatic multifunctional pilot platform system. This system includes a first input main pipe with a first input pump and a second input main pipe with a second input pump. The first input main pipe connects to multiple input pipes, and their on / off states are controlled by corresponding valves. The second input main pipe also connects to the aforementioned multiple input pipes, and their on / off states are controlled by corresponding valves. The outlet ends of the multiple input pipes are connected to the inlet ports of their respective movable mounting modules. The movable mounting modules also have multiple return ports and multiple outlet ports. The outlet ports are connected to multiple collection pipes via output pipes.

[0009] In some embodiments, the active mounting module can be used to mount a chromatography column.

[0010] In some embodiments, there are two input pipes; two corresponding liquid inlet ports; two reflux ports; two liquid outlet ports; and two output pipes.

[0011] In some embodiments, the first input main pipe and the second input main pipe are connected to the two input pipes through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding first input main pipe and the second input main pipe respectively; the other pair of opposite sides of the rectangular valve array is connected to the corresponding two input pipes respectively.

[0012] In some embodiments, two collection pipes are provided.

[0013] In some embodiments, the two output pipes are connected to two collection pipes via a second valve array; the second valve array includes four valves connected end to end to form a rectangular valve array; one set of opposite sides of the rectangular valve array is connected to the corresponding output pipes; the other set of opposite sides of the rectangular valve array is connected to the corresponding two collection pipes.

[0014] In some embodiments, the second input main pipe is connected to two collection pipes via two valves.

[0015] In some embodiments, one side of the output end of the second input pump in the second input main pipeline is connected to the first input main pipeline via a valve.

[0016] The second aspect of this utility model discloses a fully automated multifunctional chromatography system. This system includes multiple RTF chromatography columns. The inlet end of the bottom of each RTF chromatography column is connected to a corresponding inlet port, and the reflux end of the bottom of each RTF chromatography column is connected to a corresponding reflux port. The outlet end of the top of each RTF chromatography column is connected to an outlet port. Each inlet port is connected to the outlet end of a corresponding input pipe. The inlet end of each input pipe is connected to a first main input pipe equipped with a first input pump and a second main input pipe equipped with a second input pump, with the on / off state controlled by corresponding valves. Each outlet port is connected to the inlet end of a corresponding output pipe, and the outlet end of each output pipe is connected to multiple collection pipes.

[0017] In some embodiments, an air trap is provided on the first input main pipe.

[0018] In some embodiments, a mixer is provided on the first input main pipe.

[0019] In some embodiments, two RTF chromatography columns are provided.

[0020] In some embodiments, the first input main pipe and the second input main pipe are connected to the two input pipes through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding first input main pipe and the second input main pipe respectively; the other pair of opposite sides of the rectangular valve array is connected to the corresponding two input pipes respectively.

[0021] In some embodiments, the input and output pipes in each RTF chromatography column are connected by a valve.

[0022] In some embodiments, two collection pipes are provided.

[0023] In some embodiments, the two output pipes are connected to two collection pipes via a second valve array; the second valve array includes four valves connected end to end to form a rectangular valve array; one set of opposite sides of the rectangular valve array is connected to the corresponding output pipes; the other set of opposite sides of the rectangular valve array is connected to the corresponding two collection pipes.

[0024] In some embodiments, the second input main pipe is connected to two collection pipes via two valves.

[0025] In some embodiments, the inlet end of the second input main pipe is connected to the first input main pipe via a valve.

[0026] In some embodiments, the two return ports are respectively connected to corresponding return pipes, and the return pipes are respectively connected to the filter modules.

[0027] In some embodiments, the two return pipes are connected to the filter module through a third valve array group, which includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding return pipe; one side of the other pair of opposite sides of the rectangular valve array is connected to the filter module, and the other side is connected to the waste discharge pipe.

[0028] In some embodiments, the filtration module includes a main filtration input pipe, a third input pump mounted on the main filtration input pipe, one end of the main filtration input pipe connected to a third valve array, and the other end of the main filtration input pipe connected to a fourth valve array. The fourth valve array includes four valves connected end-to-end to form a rectangular valve array. The main filtration input pipe is connected to one side of a pair of opposite sides of the rectangular valve array, the other side of which is connected to a waste discharge pipe. The other pair of opposite sides of the array is connected to one end of the corresponding filter. The other ends of the two filters are connected to a fifth valve array. The fifth valve array includes four valves connected end-to-end to form a rectangular valve array. The other ends of the two filters are connected to a pair of opposite sides of the rectangular valve array. One side of the other pair of opposite sides of the rectangular valve array is connected to a liquid input pipe, and the other side of the other pair of opposite sides of the rectangular valve array is connected to the first main filtration input pipe.

[0029] In some embodiments, one side of a set of opposite sides of the fifth valve array is connected to a liquid input pipe, and the other side of a set of opposite sides of the rectangular valve array is connected to a first input main pipe and a waste discharge pipe.

[0030] The beneficial effects of this utility model are:

[0031] 1. This utility model adopts an active installation module, which can expand the platform, realize modularity, and achieve multi-functionality to meet different process requirements and meet the production processes of downstream biopharmaceuticals.

[0032] 2. This invention employs two tangential flow chromatography columns that operate alternately: one in loading mode and the other in elution mode. This allows for continuous feeding into the RTF column with minimal residue in the flow path and circulation loop, eliminating the need for repeated stops and restarts of the circulation loop.

[0033] 3. The reflux end of the tangential flow adopts a circulation loop, which drives circulation while loading the tangential flow chromatography column. Attached Figure Description

[0034] Figure 1 is a schematic diagram of the structure of this utility model.

[0035] Figure 2 is a structural diagram of the fully automated multifunctional chromatography system of this utility model. Detailed Implementation

[0036] The present invention will be further described below with reference to the accompanying drawings.

[0037] The technical content of this utility model is illustrated below through specific embodiments. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of this utility model.

[0038] Before detailing the specific embodiments of this disclosure, some terms used in this disclosure will be explained first.

[0039] Unless otherwise defined below, all technical and scientific terms used herein are intended to have the same meaning as commonly understood by one of ordinary skill in the art. References to technical terms herein refer to techniques commonly understood in the art, including variations or equivalent substitutions of techniques obvious to one of ordinary skill in the art. While it is believed that the following terms will be well understood by one of ordinary skill in the art, the following definitions are set forth to better explain this utility model. When trade names appear herein, they are intended to refer to the corresponding goods or services. All patents, published patent applications, and publications cited herein are incorporated herein by reference.

[0040] Unless otherwise stated in the text, multiple references such as "a kind of" or "the" include plural references. The expressions "a kind of or more kinds of" or "at least one kind of" can mean 1, 2, 3, 4, 5, 6, 7, 8, 9 kinds of or more kinds of.

[0041] The terms “connection,” “link,” “coupled,” or “coupled” used in this article are not limited to direct connections; they also include indirect connections.

[0042] As used in this article, “sample” refers to biomolecules, including proteins, nucleic acids, lipids, carbohydrates, small nucleotides, amino acids and their derivatives.

[0043] As used herein, "online monitoring" or "real-time monitoring" refers to the real-time detection of certain parameters or properties of the buffer solution, reaction fluid, or fluid exiting the flow reactor during the use of the chromatography system, such as pH, pressure, flow rate, and conductivity. Unlike offline detection or analysis, online or real-time monitoring provides immediate feedback on the detection results.

[0044] The A2 Buffer Storage Tank, A3 Buffer Storage Tank, A4 Buffer Storage Tank, A5 Buffer Storage Tank, P1 Storage Tank, P1-W Storage Tank, P2 Storage Tank, P2-W Storage Tank, Hot WFI Storage Tank, and Cold WFI Storage Tank mentioned in this article refer to tanks used for storing different solutions, not tanks with specific limitations. Any container that can perform the storage function is acceptable.

[0045] The positional terms "up," "down," "left," "right," "front," and "back" used in this article are determined based on the layout direction of the accompanying drawings in the specification. They are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0046] The RTF chromatography column mentioned in this article is a patented chromatography column independently developed by our company, with patent number 202220697921.8. It has a radial and tangential flow structure, which can load samples through tangential flow at the bottom of the column, while the upper part of the column head can be simultaneously flushed and eluted. It can also be circulated and cleaned with equilibration solution, thereby achieving high linear flow rate and overload loading, and achieving extremely high dynamic loading capacity.

[0047] This patent is applicable to laboratory and pilot-scale applications, with a maximum flow rate of 1000 ml / min for a single pump.

[0048] As shown in Figure 1, the multifunctional fully automatic pilot platform system includes a first input main pipe 1. The inlet end of the first input main pipe 1 is connected to the outlets of multiple liquid storage tanks, including an A2 Buffer liquid storage tank and a sample liquid storage tank. The outlets of the two liquid storage tanks are respectively equipped with a first valve 106 and a second valve 104. From left to right, the first input main pipe 1 is equipped with a first input pump 2, a first pressure sensor 3, a first flow meter 4, a third valve 5, a mixer 6, and an air trap 7. The inlet end of the air trap 7 is connected to the first input main pipe 1 through a fourth valve 8, and the outlet end of the air trap 7 is connected to the first input main pipe 1 through a fifth valve 9. A sixth valve 10 is installed on the pipes at the inlet and outlet ends of the air trap 7. The second input main pipe 11 has its inlet connected to the outlets of multiple storage tanks, including A3 Buffer Storage Tank, A4 Buffer Storage Tank, and A5 Buffer Storage Tank. The outlets of the three storage tanks are respectively equipped with a seventh valve 102, an eighth valve 101, and a ninth valve 103. The outlet of the A3 Buffer Storage Tank is connected to the inlet of the first input main pipe 1 via a tenth valve 105. From left to right, the second input main pipe 11 is equipped with a second input pump 12, a second pressure sensor 13, a second flow meter 14, an eleventh valve 15, and a twelfth valve 16. The first input main pipe 1 and the second input main pipe 11 are connected via a thirteenth valve 17, one end of which is connected between the third valve 5 and the mixer 6, and the other end is connected between the second flow meter 14 and the eleventh valve 15.

[0049] The first valve array 17 is a rectangular valve array formed by four valves connected end to end, as shown in the figure, in a clockwise direction: fourteenth valve 18, fifteenth valve 19, sixteenth valve 20, and seventeenth valve 21. The outlet end of the first main input pipe 1 is connected between fourteenth valve 18 and seventeenth valve 21. The outlet end of the twelfth valve 16 of the second main input pipe 11 is connected between fifteenth valve 19 and sixteenth valve 20. One end of the first input pipe 22 is connected between fourteenth valve 18 and fifteenth valve 19, and the other end of the first input pipe 22 is connected to the first inlet port 24 of the movable mounting module 23. An eighteenth valve 25 is provided on the first input pipe 22. One end of the second input pipe 26 is connected between sixteenth valve 20 and seventeenth valve 21, and the other end of the second input pipe 26 is connected to the second inlet port 27 of the movable mounting module 23. A nineteenth valve 29 is provided on the second input pipe 26.

[0050] The movable installation module 23 further includes a first return port 30, a second return port 31, a first outlet port 32, and a second outlet port 33. The first outlet port 32 is connected to one end of the first output pipe 34, and the second outlet port 33 is connected to one end of the second output pipe 35. The other ends of the first output pipe 34 and the second output pipe 35 are respectively connected to the second valve array group 36. The second valve array group 36 is a rectangular valve array formed by four valves connected end to end, as shown in the figure, in a clockwise direction: the twentieth valve 37, the twenty-first valve 38, the twenty-second valve 39, and the twenty-third valve 40. The other end of the first output pipe 34 is connected between the twenty-first valve 38 and the twenty-second valve 39, and the other end of the second output pipe 35 is connected between the twentieth valve 37 and the twenty-third valve 40. The 20th valve 37 and the 21st valve 38 are connected to the inlet end of the first collection pipe 41; the 22nd valve 39 and the 23rd valve 40 are connected to the inlet end of the second collection pipe 42; the first collection pipe 41 is connected to the P1 storage tank and the P1-W storage tank via the 24th valve 43 and the 25th valve 44 respectively. The second collection pipe 42 is connected to the P2 storage tank and the P2-W storage tank via the 26th valve 45 and the 27th valve 46 respectively. The outlet of the 12th valve 16 of the second input main pipe 11 is connected to the first collection pipe 41 and the second collection pipe 42 via the first intermediate pipe connection 47 and the second intermediate pipe 48 respectively. The two ends of the first intermediate pipe 48 are respectively equipped with the 28th valve 49 and the 29th valve 50; the two ends of the second intermediate pipe 48 are respectively equipped with the 30th valve 51 and the 31st valve 52.

[0051] The first output pipe 34 is equipped with the thirty-second valve 53. The second output pipe 35 is equipped with the thirty-third valve 54.

[0052] The first return port 30 of the active installation module 23 is connected to the inlet of the filter module 56 through the thirty-fourth valve 55; the second return port 31 of the active installation module 23 is connected to the inlet of the filter module 56 through the thirty-fifth valve 57; the outlet of the filter module 56 is connected between the third valve 5 of the first input main pipe 1 and the mixer 6 through the thirty-sixth valve 58.

[0053] Referring to Figure 2, a fully automated multifunctional chromatography system is provided, in which two RTF chromatography columns are installed on the aforementioned pilot platform system.

[0054] The system includes a first input main pipe 1, the inlet of which is connected to the outlets of multiple storage tanks, including an A2 Buffer storage tank and a sample storage tank; a first valve 106 and a second valve 104 are respectively installed at the outlets of the two storage tanks; from left to right, the first input main pipe 1 is equipped with a first input pump 2, a first pressure sensor 3, a first flow meter 4, a third valve 5, a mixer 6, and an air trap 7; the inlet of the air trap 7 is connected to the first input main pipe 1 through a fourth valve 8, and the outlet of the air trap 7 is connected to the first input main pipe 1 through a fifth valve 9; a sixth valve 10 is installed on the pipes at the inlet and outlet of the air trap 7. The second input main pipe 11 has its inlet connected to the outlets of multiple storage tanks, including A3 Buffer Storage Tank, A4 Buffer Storage Tank, and A5 Buffer Storage Tank. The outlets of the three storage tanks are respectively equipped with a seventh valve 102, an eighth valve 101, and a ninth valve 103. The outlet of the A3 Buffer Storage Tank is connected to the inlet of the first input main pipe 1 via a tenth valve 105. From left to right, the second input main pipe 11 is equipped with a second input pump 12, a second pressure sensor 13, a second flow meter 14, an eleventh valve 15, and a twelfth valve 16. The first input main pipe 1 and the second input main pipe 11 are connected via a thirteenth valve 17, one end of which is connected between the third valve 5 and the mixer 6, and the other end is connected between the second flow meter 14 and the eleventh valve 15.

[0055] The first valve array group 17 is a rectangular valve array formed by four valves connected end to end, as shown in the figure, in a clockwise direction: fourteenth valve 18, fifteenth valve 19, sixteenth valve 20, and seventeenth valve 21. The outlet end of the first input main pipe 1 is connected between fourteenth valve 18 and seventeenth valve 21. The outlet end of the twelfth valve 16 of the second input main pipe 11 is connected between fifteenth valve 19 and sixteenth valve 20. One end of the first input pipe 22 is connected between fourteenth valve 18 and fifteenth valve 19. The other end of the first input pipe 22 is connected to the bottom inlet of the first RTF chromatography column 65 through the first inlet port 24 of the movable mounting module 23. An eighteenth valve 25 is provided on the first input pipe 22. One end of the second input pipe 26 is connected between sixteenth valve 20 and seventeenth valve 21. The other end of the second input pipe 26 is connected to the inlet end of the second RTF chromatography column 66 through the second inlet port 27 of the movable mounting module 23. A nineteenth valve 29 is provided on the second input pipe 26.

[0056] The movable installation module 23 further includes a first reflux port 30, a second reflux port 31, a first liquid outlet port 32, and a second liquid outlet port 33. The first RTF chromatography column 65 is connected to one end of the first output pipe 34 through the first liquid outlet port 32, and the second RTF chromatography column 66 is connected to one end of the second output pipe 35 through the second liquid outlet port 33. The other ends of the first output pipe 34 and the second output pipe 35 are respectively connected to the second valve array group 36. The second valve array group 36 is a rectangular valve array formed by four valves connected end to end, as shown in the figure, in a clockwise direction: the twentieth valve 37, the twenty-first valve 38, the twenty-second valve 39, and the twenty-third valve 40. The other end of the first output pipe 34 is connected between the twenty-first valve 38 and the twenty-second valve 39, and the other end of the second output pipe 35 is connected between the twentieth valve 37 and the twenty-third valve 40. The 20th valve 37 and the 21st valve 38 are connected to the inlet end of the first collection pipe 41; the 22nd valve 39 and the 23rd valve 40 are connected to the inlet end of the second collection pipe 42; the first collection pipe 41 is connected to the P1 storage tank and the P1-W storage tank via the 24th valve 43 and the 25th valve 44 respectively. The second collection pipe 42 is connected to the P2 storage tank and the P2-W storage tank via the 26th valve 45 and the 27th valve 46 respectively. The outlet of the 12th valve 16 of the second input main pipe 11 is connected to the first collection pipe 41 and the second collection pipe 42 via the first intermediate pipe connection 47 and the second intermediate pipe 48 respectively. The two ends of the first intermediate pipe 48 are respectively equipped with the 28th valve 49 and the 29th valve 50; the two ends of the second intermediate pipe 48 are respectively equipped with the 30th valve 51 and the 31st valve 52.

[0057] The first output pipe 34 is equipped with a thirty-second valve 53 and a thirty-seventh valve 59. The second output pipe 35 is equipped with a thirty-third valve 54 and a thirty-eighth valve 60. A first connecting pipe 61 is provided between the first input pipe 22 and the first output pipe 34. One end of the first connecting pipe 61 connects between the eighteenth valve 25 and the first valve array group 17, and the other end connects between the thirty-second valve 53 and the thirty-seventh valve 59. A second connecting pipe 62 is provided between the second input pipe 26 and the second output pipe 35. One end of the second connecting pipe 62 connects between the nineteenth valve 29 and the first valve array group 17, and the other end connects between the thirty-third valve 54 and the thirty-eighth valve 60. The first connecting pipe 61 and the second connecting pipe 62 are respectively equipped with a thirty-ninth valve 63 and a fortieth valve 64.

[0058] The bottom reflux port of the first RTF chromatography column 65 is connected to the third valve array group 67 via the first reflux port 30, and the bottom reflux port of the second RTF chromatography column 66 is connected to the third valve array group 68 via the second reflux port 31. The third valve array group 67 is a rectangular valve array formed by four valves connected end-to-end, as shown in the figure, in a clockwise direction: valve 69 (41), valve 70 (42), valve 71 (43), and valve 72 (44). The bottom reflux port of the first RTF chromatography column 65 connects between valve 69 (41) and valve 72 (44); the bottom reflux port of the second RTF chromatography column 66 connects between valve 70 (42) and valve 71 (43). One end of the filter input main pipe 73 is connected between valve 71 (43) and valve 72 (44); a waste discharge pipe is connected between valve 69 (41) and valve 70 (42). The other end of the filter input main pipe 73 is connected to the fourth valve array group 74. From right to left, the filter input main pipe 73 is equipped with a third input pump 75, a third flow meter 76, a third pressure sensor 77, and a forty-fifth valve 78. The fourth valve array group 74 is a rectangular valve array formed by four valves connected end-to-end, as shown in the figure, in a clockwise direction: forty-sixth valve 79, forty-seventh valve 80, forty-eighth valve 81, and forty-nineth valve 82. The other end of the filter input main pipe 73 is connected between forty-seventh valve 80 and forty-eighth valve 81; a waste discharge pipe is connected between forty-sixth valve 79 and forty-nineth valve 82. The bottom end of the first filter 83 is connected between forty-sixth valve 79 and forty-seventh valve 80, and the end of the second filter 84 is connected between forty-eighth valve 81 and forty-nineth valve 82. The tops of the first filter 83 and the second filter 84 are respectively connected to the fifth valve array group 85. The fifth valve array group 85 is a rectangular valve array formed by four valves connected end to end, as shown in the figure, in a clockwise direction: 50th valve 86, 51st valve 87, 52nd valve 88, and 53rd valve 89. The top of the first filter 83 is connected between the 50th valve 86 and the 51st valve 87, and the top of the second filter 84 is connected between the 52nd valve 88 and the 53rd valve 89. The outlet of the liquid inlet pipe 90 is connected between the 50th valve 86 and the 53rd valve 89. The inlet of the inlet pipe 90 is connected to the hot WFI storage tank and the cold WFI storage tank, respectively. The waste discharge pipe and the first main input pipe 1 are connected by pipes between the third valve 5 and the mixer 6. The 54th valve 91 and the 55th valve 92 are respectively installed on the pipes.

[0059] A UV sensor 93, a first conductivity sensor 94, and a first pH sensor 95 are respectively installed on the first collection pipe 41. A second conductivity sensor 96 and a second pH sensor 97 are respectively installed on the second collection pipe 42.

Claims

1. A fully automatic multi-functional pilot platform system, characterized in that: The platform system includes a first input main pipe with a first input pump and a second input main pipe with a second input pump. The first input main pipe connects to multiple input pipes, and their on / off states are controlled by corresponding valves. The second input main pipe also connects to the aforementioned multiple input pipes, and their on / off states are controlled by corresponding valves. The outlet ends of the multiple input pipes are connected to the inlet ports of their respective movable mounting modules. The movable mounting modules are also equipped with multiple reflux ports and multiple outlet ports. The outlet ports are connected to multiple collection pipes via output pipes.

2. The fully automated multi-functional pilot platform system according to claim 1, characterized in that: The aforementioned active mounting module can be used to mount chromatography columns.

3. The fully automated multi-functional pilot platform system according to claim 1, characterized in that: The system has two input pipes; two corresponding inlet ports; two reflux ports; two outlet ports; and two output pipes.

4. The fully automated multi-functional pilot platform system according to claim 3, characterized in that: The first input main pipe and the second input main pipe are connected to the two input pipes through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding first input main pipe and the second input main pipe respectively; the other pair of opposite sides of the rectangular valve array is connected to the corresponding two input pipes respectively.

5. The fully automated multi-functional pilot platform system according to claim 1 or 4, characterized in that: There are two collection pipes.

6. The fully automated multi-functional pilot platform system according to claim 5, characterized in that: The two output pipes are connected to two collection pipes through a second valve array; the second valve array includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding output pipes; the other pair of opposite sides of the rectangular valve array is connected to the corresponding two collection pipes.

7. The fully automated multi-functional pilot platform system according to claim 5, characterized in that: The outlet end of the second input main pipe is connected to two collection pipes via two valves.

8. The fully automated multi-functional pilot platform system according to claim 5, characterized in that: The output end of the second input pump in the second input main pipeline is connected to the first input main pipeline via a valve.

9. A fully automated multifunctional chromatography system based on any one of the fully automated multifunctional pilot platform systems of claims 1-8, characterized in that: The chromatographic system includes multiple RTF chromatography columns. The inlet end of the bottom of each RTF chromatography column is connected to a corresponding inlet port, and the reflux end of the bottom of each RTF chromatography column is connected to a corresponding reflux port. The outlet end of the top of each RTF chromatography column is connected to an outlet port. Each inlet port is connected to the outlet end of a corresponding input pipe. The inlet end of each input pipe is connected to a first main input pipe equipped with a first input pump and a second main input pipe equipped with a second input pump, and the on / off state is controlled by corresponding valves. Each outlet port is connected to the inlet end of a corresponding output pipe, and the outlet end of each output pipe is connected to multiple collection pipes.

10. The fully automated multifunctional chromatography system according to claim 9, characterized in that: An air trap is provided on the first input main pipe.

11. The fully automated multifunctional chromatography system according to claim 9, characterized in that: A mixer is installed on the first input main pipe.

12. The fully automated multifunctional chromatography system according to claim 9, characterized in that: Two RTF chromatography columns are provided.

13. The fully automated multifunctional chromatography system according to claim 12, characterized in that: The first input main pipe and the second input main pipe are connected to the two input pipes through a first valve array group; the first valve array group includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding first input main pipe and the second input main pipe respectively; the other pair of opposite sides of the rectangular valve array is connected to the corresponding two input pipes respectively.

14. The fully automated multifunctional chromatography system according to claim 12, characterized in that: The input and output pipes in each RTF chromatography column are connected by a valve.

15. The fully automated multifunctional chromatography system according to claim 9, characterized in that: There are two collection pipes.

16. The fully automated multifunctional chromatography system according to claim 15, characterized in that: The two output pipes are connected to two collection pipes through a second valve array; the second valve array includes four valves connected end to end to form a rectangular valve array; one pair of opposite sides of the rectangular valve array is connected to the corresponding output pipes; the other pair of opposite sides of the rectangular valve array is connected to the corresponding two collection pipes.

17. The fully automated multifunctional chromatography system according to claim 16, characterized in that: The second input main pipe is connected to two collection pipes through two valves.

18. The fully automated multifunctional chromatography system according to claim 9, characterized in that: The inlet end of the second input main pipe is connected to the first input main pipe via a valve.

19. The fully automated multifunctional chromatography system according to claim 9, characterized in that: The two reflux ports of the RTF chromatography column are respectively connected to corresponding reflux pipes, and the reflux pipes are respectively connected to the filter module.

20. The fully automated multifunctional chromatography system according to claim 19, characterized in that: The two return pipes are connected to the filter module through a third valve array. The third valve array includes four valves connected end to end to form a rectangular valve array. One set of opposite sides of the rectangular valve array is connected to the corresponding return pipe. One side of the other set of opposite sides of the rectangular valve array is connected to the filter module, and the other side is connected to the waste discharge pipe.

21. The fully automated multifunctional chromatography system according to claim 20, characterized in that: The filtration module includes a main filtration input pipe with a third input pump. One end of the main filtration input pipe is connected to a third valve array, and the other end is connected to a fourth valve array. The fourth valve array consists of four valves connected end-to-end to form a rectangular valve array. The main filtration input pipe is connected to one side of a pair of opposite sides of the rectangular valve array, with the other side connected to a waste discharge pipe. The other pair of opposite sides of the rectangular valve array is connected to one end of the corresponding filter. The other ends of the two filters are connected to a fifth valve array. The fifth valve array consists of four valves connected end-to-end to form a rectangular valve array. The other ends of the two filters are connected to a pair of opposite sides of the rectangular valve array. One side of the other pair of opposite sides of the rectangular valve array is connected to a liquid input pipe, and the other side of the other pair of opposite sides of the rectangular valve array is connected to the first main input pipe.

22. The fully automated multifunctional chromatography system according to claim 21, characterized in that: One side of a pair of opposite sides of the fifth valve array is connected to the liquid input pipe, and the other side of a pair of opposite sides of the fifth valve array is connected to the first input main pipe and the waste discharge pipe.

Images