Cosmetic composition for improving photoaging and thermal aging, and manufacturing method therefor

Abstract

The present invention relates to a cosmetic composition for improving photoaging and thermal aging, and a manufacturing method therefor, the cosmetic composition comprising a micelle formed by ion pairs resulting from hydrogen bonding between arginine and at least one saturated C8-12 fatty acid and / or a micelle formed by ion pairs resulting from hydrogen bonding between arginine and at least one triglyceride containing at least one saturated C8-12 fatty acid, thereby increasing the water solubility of arginine, which is a zwitterion, and skin penetration thereof through the stratus corneum and having synergistically strengthened and enhanced effects of reduced inflammation caused by photoaging and thermal aging, alleviation of aging symptoms, and skin improvement.

Description

Cosmetic composition for improving photoaging and thermal aging and method for manufacturing the same

[0001] The present invention relates to a cosmetic composition for improving photoaging and thermal aging, and a method for manufacturing the same, for improving skin inflammation or photoaging caused by a light source such as ultraviolet rays, and skin inflammation or thermal aging caused by a heat source such as infrared rays. More specifically, the invention relates to a micelle formed by an ion pair formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or a micelle formed by an ion pair formed by hydrogen bonding between arginine and one or more triglycerides containing 8 to 12 carbon atoms, wherein the composition possesses zwitterion characteristics and includes a guanidyl group to increase the penetration of arginine, which is difficult to penetrate through the stratum, through the stratum, thereby having improved effects on photoaging and thermal aging, and providing complex effects on promoting skin regeneration and alleviating inflammation. The present invention relates to a cosmetic composition for improving photoaging and thermal aging having synergistic effects and a method for manufacturing the same.

[0002] The skin is the largest tissue in the body and serves as the primary organ for protecting and contacting the body against the external environment and stimuli. It not only acts as the first line of defense against external substances and stimuli but also plays a crucial role in internal metabolism by regulating body temperature and fluid balance. Furthermore, as the first organ visible to the outside, the skin is a vital component in expressing external beauty. In modern society, this emphasis on external beauty is increasingly becoming as significant as the emphasis on health. This is because, with the rising elderly population, physical beauty has established itself as a criterion symbolizing youth and health; moreover, for the younger generation, this external beauty manifests as confidence and serves as a competitive edge that influences their professional performance. For these reasons, active efforts are being made in both academic and industrial fields to prevent skin damage and aging.

[0003] While the sun is an essential element for the survival of living organisms, excessive exposure to ultraviolet (UV) radiation, due to its high energy, can cause short-term dilation of capillaries in the epidermal layer, leading to erythema, heat sensation, pain, swelling, blistering, and epidermal peeling. It also promotes melanin production, causing tanning. Furthermore, increased skin sensitivity can induce rashes and itching. Chronic exposure causes significant degenerative changes in skin cells, fibrous tissue, and blood vessels, leading to premature skin aging and skin cancer. Specific signs of aging include decreased skin elasticity, wrinkles, and dry, rough skin resulting from a weakened skin barrier. Increased melanin production also causes freckles, moles, dark spots, and brown pigmentation. Recent studies indicate that even short-term exposure to UV radiation can induce inflammation in skin cells.

[0004] Furthermore, it has recently been reported that thermal energy from infrared rays, which had not previously been identified as a risk, can also cause aging at a level similar to that of ultraviolet rays. In addition, as it has been revealed that thermal aging occurs in high-temperature and dry environments caused by heating, as well as in environments exposed to temperatures above 43 degrees Celsius during showering, awareness of this issue is rising.

[0005] Recently, due to rapid climate change and environmental pollution, there has been increased exposure to ultraviolet (UV) and infrared (IR) rays. This increased exposure leads to photoaging and thermal aging, prompting the release of various functional cosmetics designed to prevent inflammation caused by these factors or to alleviate symptoms of aging. However, conventional functional cosmetics currently face limitations in effectively mitigating inflammatory factors that increase specifically in response to UV or IR rays, or in their application due to the low stability or poor solubility of specific ingredients designed to eliminate inflammation-causing Reactive Oxygen Species (ROS). Furthermore, conventional ingredients designed to eliminate ROS are not efficiently absorbed into the skin, limiting their ability to suppress inflammation caused by UV and IR rays. Additionally, there have been issues where skin pigmentation caused by melanin, weakened skin elasticity, and dryness resulting from a weakened skin barrier were not effectively resolved.

[0006] In other words, conventionally known functional cosmetic compositions for improving photoaging and thermal aging have problems such as limitations in usage amounts due to the stability and physical properties of the applied ingredients, or the inability to expect substantial skin improvement effects because their penetration through the stratum corneum is negligible. Therefore, there is an urgent need to develop a new cosmetic composition for improving photoaging and thermal aging, as well as a method for manufacturing the same. This composition exhibits higher stability than existing functional cosmetic compositions, enabling high-level application in functional cosmetic compositions to demonstrate effective skin improvement; facilitates easy penetration through the stratum corneum for effective delivery into the skin; suppresses inflammation caused by ultraviolet and infrared rays; and possesses a complex synergistic effect that promotes skin regeneration as a whole, rather than when individual ingredients are used alone.

[0007] Furthermore, in the case of conventional technology, there are limitations in that arginine has difficulty penetrating the skin through the stratum corneum due to its zwitterionic properties, and exhibits high alkalinity when used in functional cosmetic compositions due to the characteristics of the guanidyl group, making the use of a neutralizing agent essential. Consequently, when used in functional cosmetic compositions, stability is poor, resulting in insufficient anti-inflammatory effects; additionally, the use of neutralizing agents limits the ability to effectively improve photoaging and thermal aging.

[0008] In addition, in the case of conventional technology, when using poorly soluble ingredients such as betulin or quercetin in functional cosmetic compositions, there was a problem that there were limitations in applying them to water-soluble formulations of cosmetics (skin toners, ampoules, essences, etc.) due to their low solubility in water, despite the high antioxidant effects resulting from triterpene and flavonol components, respectively. Furthermore, there was a problem that there were limitations in applying a complex mixture of betulin or quercetin and arginine to the skin, as they are effective in reducing inflammation caused by photoaging and thermal aging and thereby alleviating aging symptoms, but have poor compatibility with each other. Therefore, there is a great need to develop a new technology in which micelles are formed by ion pairs formed by hydrogen bonding between one or more of saturated fatty acids having 8 to 12 carbon atoms and arginine, or micelles are formed by ion pairs formed by hydrogen bonding between one or more of triglycerides containing one or more of saturated fatty acids having 8 to 12 carbon atoms and arginine, and nanovesicles are formed by adding a sparingly soluble component such as betulin or quercetin inside the micelles. Through the formation of these nanovesicles, zwitterion arginine, insoluble components such as betulin or quercetin can be effectively delivered into the skin through the keratin.In addition, by encapsulating poorly soluble ingredients such as betulin or quercetin inside nanovesicles, the solubility in water is increased, and the poorly soluble ingredients such as betulin or quercetin can be easily applied to water-soluble formulations of cosmetics (skin toners, ampoules, essences, etc.). Furthermore, since it is possible to complexly mix betulin or quercetin with arginine, which have poor compatibility with each other, it can have the characteristic of expecting a greater complex synergistic effect in reducing inflammation caused by photoaging and thermal aging, thereby alleviating aging symptoms and improving the skin, compared to the single composition of betulin or quercetin or arginine alone.

[0009] Korean Patent Publication No. 10-2022-0113587 (published August 16, 2022) disclosed cosmetic and pharmaceutical compositions containing carnitine, arginine, creatine, and vitamin C as active ingredients. The invention relates to a cosmetic composition containing carnitine, arginine, creatine, and vitamin C as active ingredients. A cosmetic and pharmaceutical composition of one aspect exhibits low skin irritation and excellent skin stability, and can demonstrate anti-aging or wrinkle improvement effects by promoting collagen biosynthesis and inhibiting the production of collagen-degrading enzymes. Furthermore, it possesses the efficacy to repair damaged cells, and by promoting skin cell regeneration, it can regenerate the skin, thereby effectively improving the skin barrier. However, cosmetic and pharmaceutical compositions containing the above-mentioned carnitine, arginine, creatine, and vitamin C as active ingredients do not solve the problem of arginine being difficult to penetrate the skin through the keratin, making it difficult to substantially expect anti-aging or wrinkle-improving effects on the skin. Additionally, there is a problem in that it is difficult to expect complex synergistic effects for reducing inflammation caused by photoaging and thermal aging, alleviating aging symptoms, and improving the skin by using micelles formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or micelles formed by hydrogen bonding between arginine and one or more triglycerides containing 8 to 12 carbon atoms.

[0010] Accordingly, the inventors have invented a cosmetic composition for improving photoaging and thermal aging and a method for preparing the same, which can improve the penetration of arginine, a zwitterion, through the keratin of the skin by forming micelles by ion pairs formed by hydrogen bonding between one or more of saturated fatty acids having 8 to 12 carbon atoms and arginine, or by forming micelles by ion pairs formed by hydrogen bonding between one or more of triglycerides containing one or more of saturated fatty acids having 8 to 12 carbon atoms and arginine. Furthermore, a cosmetic composition for improving photoaging and thermal aging and a method for manufacturing the same were invented, wherein in the process of manufacturing a nanovesicle by adding a poorly soluble component to the micelle, a poorly soluble betulin or quercetin is loaded inside the nanovesicle, thereby facilitating the application of the poorly soluble component in the cosmetic composition, and maintaining stability within the water-soluble formulation of the cosmetic (skin toner, ampoule, essence, etc.) even though the nanovesicle has a diameter of several hundred nanometers. As a result, the inventors have developed a cosmetic composition for improving photoaging and thermal aging and a method for preparing the same, which enhances the effective delivery of zwitterion arginine, poorly soluble components such as betulin or quercetin into the skin through the keratin by forming micelles and nanovesicles, can be easily applied to water-soluble formulations of cosmetics (skin toners, ampoules, essences, etc.), and has a greater complex synergistic effect in reducing inflammation caused by photoaging and thermal aging within skin cells, thereby alleviating aging symptoms and improving the skin.

[0011] The present invention was devised to solve the above problems, and the objective of the present invention is to provide a cosmetic composition for improving photoaging and thermal aging and a method for manufacturing the same, which has improved effects in reducing inflammation caused by photoaging and thermal aging, including itching, and protecting the skin by increasing the water solubility of arginine, which is a zwitterion, by utilizing a micelle structure formed by an ion pair formed by hydrogen bonding between arginine and one or more of saturated fatty acids having 8 to 12 carbon atoms, or a micelle structure formed by an ion pair formed by hydrogen bonding between arginine and one or more of triglycerides containing 8 to 12 carbon atoms, even when the same amount is applied to the skin.

[0012] In addition, another objective of the present invention is to provide a cosmetic composition for improving photoaging and thermal aging and a method for preparing the same, which utilizes a micelle structure formed by ion pairs formed by hydrogen bonding between arginine and one or more of saturated fatty acids having 8 to 12 carbon atoms, or a micelle structure formed by ion pairs formed by hydrogen bonding between arginine and one or more of triglycerides containing 8 to 12 carbon atoms, thereby increasing the penetration of arginine, which is a zwitterion, through the keratin of the skin and having increased effects in reducing inflammation caused by photoaging and thermal aging, alleviating symptoms of aging, and improving the skin.

[0013] In addition, another objective of the present invention is to utilize a nanovesicle structure formed by adding a sparingly soluble component, such as betulin or quercetin, inside a micelle, wherein a micelle is formed by an ion pair formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or a micelle is formed by an ion pair formed by hydrogen bonding between arginine and one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms, thereby enhancing the water solubility of the sparingly soluble component, such as betulin or quercetin, and wherein the sparingly soluble component, betulin or quercetin, is used in a water-soluble formulation of a cosmetic (skin toner, ampoule, etc. The present invention provides a cosmetic composition for improving photoaging and thermal aging that can be easily applied to essences, etc., and a method for manufacturing the same.

[0014] In addition, another objective of the present invention is to provide a cosmetic composition for improving photoaging and thermal aging that utilizes a nanovesicle structure formed by adding a sparingly soluble component such as betulin or quercetin inside the micelle, wherein a micelle is formed by an ion pair formed by hydrogen bonding between one or more saturated fatty acids having 8 to 12 carbon atoms and arginine, or a micelle is formed by an ion pair formed by hydrogen bonding between one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms and arginine, thereby increasing the penetration of the sparingly soluble component such as betulin or quercetin through the keratin and having an increased effect on reducing inflammation caused by photoaging and thermal aging, alleviating aging symptoms, and improving the skin. It is to provide a manufacturing method.

[0015] In addition, another objective of the present invention is to provide a cosmetic composition for improving photoaging and thermal aging and a method for manufacturing the same, which allows for the combined use of arginine, a zwitterion, and a poorly soluble component such as betulin or quercetin, which has poor compatibility with arginine, a zwitterion, thereby reducing side effects compared to when arginine, a zwitterion, and a poorly soluble component such as betulin or quercetin are used individually, and having a combined enhanced and synergistic effect for reducing inflammation caused by photoaging and thermal aging, alleviating symptoms of aging, and improving the skin.

[0016] To achieve the above objectives, a cosmetic composition for improving photoaging and thermal aging according to one embodiment of the present invention may be characterized by including at least one micelle formed by hydrogen bonding between arginine and at least one of a saturated fatty acid having 8 to 12 carbon atoms, or at least one micelle formed by hydrogen bonding between arginine and at least one of a triglyceride containing at least one of a saturated fatty acid having 8 to 12 carbon atoms.

[0017] In this case, in a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention, the micelles may be characterized by having an average diameter of 1-10 nm.

[0018] In this case, a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized by additionally including a sparingly soluble component inside the micelle structure to form nanovesicles.

[0019] In this case, in a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention, the nanovesicle may be characterized by having an average diameter of 100-500 nm.

[0020] In this case, the cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized in that the insoluble component includes one or more of betulin or quercetin.

[0021] In this case, in a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention, the saturated fatty acid having 8 to 12 carbon atoms may be characterized as having a carboxyl group only at the 1st carbon and having a linear structure rather than a branched structure.

[0022] In this case, in a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention, the saturated fatty acid having a linear structure with 8 to 12 carbon atoms may be characterized as being caprylic acid, capric acid, or lauric acid.

[0023] In this case, a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized by additionally including a linear diol having 6 or more and 12 or fewer carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure.

[0024] In this case, the linear diol in the cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized as being 1,2-hexanediol.

[0025] In this case, a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized by including micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, wherein the insoluble component is betulin, and the arginine is 0.1 to 20 parts by weight, the caprylic acid is 0.098 to 20 parts by weight, the betulin is 0.002 to 0.5 parts by weight, and the water is 59.5 to 99.8 parts by weight.

[0026] In this case, a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized by including micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, wherein the insoluble component is quercetin, and the arginine is 0.1 to 20 parts by weight, the caprylic acid is 0.098 to 20 parts by weight, the quercetin is 0.002 to 2 parts by weight, and the water is 58 to 99.8 parts by weight.

[0027] In this case, a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized by including micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the caprylic acid and the arginine having a molar ratio of 1:3 to 3:1.

[0028] In this case, in a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention, the triglyceride containing one or more of the saturated fatty acids having 8 to 12 carbon atoms may be characterized as being glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride.

[0029] In this case, a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized by additionally including a linear diol having 6 or more and 12 or fewer carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure.

[0030] In this case, the linear diol in the cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may be characterized as being 1,2-hexanediol.

[0031] Meanwhile, a method for preparing a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention may comprise the steps of: adding 0.1 to 20 parts by weight of arginine to 59.5 to 99.8 parts by weight of water and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; and adding 0.098 to 20 parts by weight of caprylic acid to the first solution while heating to 50°C to 90°C and stirring with a homodisper at 2,000 rpm to 4,000 rpm for 10 to 20 minutes to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine. there is.

[0032] Meanwhile, a method for preparing a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention comprises the steps of: adding 0.1 to 20 parts by weight of arginine to 59.5 to 99.8 parts by weight of water and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; adding 0.002 to 0.5 parts by weight of betulin and 0.098 to 20 parts by weight of caprylic acid to 50℃ to 90℃ while stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a seconda solution in which betulin is dissolved in caprylic acid; and The method may be configured to include the step of adding the seconda solution to the first solution, heating it to 50°C - 90°C, stirring it at 2,000 rpm - 4,000 rpm for 10 - 30 minutes using a homodisper, thereby forming micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the step of forming nanovesicles by including the betulin inside the micelle structure.

[0033] Meanwhile, a method for preparing a cosmetic composition for improving photoaging and thermal aging according to another embodiment of the present invention comprises the steps of: adding 0.1 to 20 parts by weight of arginine to 58 to 99.8 parts by weight of water and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; adding 0.002 to 2 parts by weight of quercetin and 0.098 to 20 parts by weight of caprylic acid to 50℃ to 90℃ while stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a secondb solution in which quercetin is dissolved in caprylic acid; and The method may be configured to include the step of adding the above-mentioned 2b solution to the first solution, heating it to 50°C - 90°C, stirring it at 2,000 rpm - 4,000 rpm for 10 - 30 minutes using a homodisper to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the step of forming nanovesicles by including the quercetin inside the micelle structure.

[0034] The cosmetic composition for improving photoaging and thermal aging and the method for manufacturing the same according to the present invention have the following effects.

[0035] First, the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for preparing the same utilize a micelle structure formed by an ion pair formed by hydrogen bonding between arginine and one or more of saturated fatty acids having 8 to 12 carbon atoms, or a micelle structure formed by an ion pair formed by hydrogen bonding between arginine and one or more of triglycerides containing 8 to 12 carbon atoms, thereby increasing the solubility of arginine, which is a zwitterion, in water, so that even when the same amount is applied to the skin, it has an improved effect in reducing inflammation caused by photoaging and thermal aging, including itching, and protecting the skin.

[0036] Second, the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for preparing the same utilize a micelle structure formed by an ion pair formed by hydrogen bonding between arginine and one or more of saturated fatty acids having 8 to 12 carbon atoms, or a micelle structure formed by an ion pair formed by hydrogen bonding between arginine and one or more of triglycerides containing 8 to 12 carbon atoms, thereby increasing the penetration of arginine, which is a zwitterion, through the keratin of the skin and having an increased effect on reducing inflammation caused by photoaging and thermal aging, alleviating aging symptoms, and improving the skin.

[0037] Third, the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for preparing the same utilize a nanovesicle structure formed by adding a sparingly soluble component such as betulin or quercetin inside the micelle, wherein a micelle is formed by an ion pair formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or a micelle is formed by an ion pair formed by hydrogen bonding between arginine and one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms, thereby enhancing the water solubility of the sparingly soluble component such as betulin or quercetin, and the sparingly soluble component betulin or quercetin It has the effect of being easily applied to water-soluble formulations of cosmetics (skin toner, ampoule, essence, etc.).

[0038] Fourth, the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for preparing the same utilize a nanovesicle structure formed by adding a sparingly soluble component such as betulin or quercetin inside the micelle, wherein micelles are formed by ion pairs formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or micelles are formed by ion pairs formed by hydrogen bonding between arginine and one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms, thereby increasing the penetration of sparingly soluble components such as betulin or quercetin through the keratin and thereby reducing inflammation caused by photoaging and thermal aging, alleviating aging symptoms, and improving the skin. It has an increased effect.

[0039] Fifth, the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for manufacturing the same make it possible to use in combination a zwitterion arginine with a poorly soluble component such as betulin or quercetin, which has poor compatibility with arginine. This reduces side effects compared to when the zwitterion arginine and the poorly soluble component such as betulin or quercetin are used individually, and provides a combined, enhanced, and synergistic effect for reducing inflammation caused by photoaging and thermal aging, alleviating aging symptoms, and improving the skin.

[0040] Figure 1 is a diagram showing the formation of ion pairs by two strong hydrogen bonds between the guanidyl group of arginine and the carboxyl group of caprylic acid in the cosmetic composition for improving photoaging and thermal aging according to the present invention, and the formation of micelles by self-assembly.

[0041] FIG. 2 is a flowchart illustrating a method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention.

[0042] FIG. 3 is another flowchart illustrating a method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention.

[0043] FIG. 4 is another flowchart illustrating a method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention.

[0044] Figure 5 is a diagram showing the change in pH as the content of caprylic acid is increased from 1% to 12% while the content of arginine is fixed (10%).

[0045] Figure 6 is a photograph comparing the characteristics of a micelle formed by an ion pair formed by hydrogen bonding between caprylic acid and arginine in Example 1 and a nanovesicle formed by containing betulin inside the micelle structure in Example 2.

[0046] Figure 7 is a comparison of electron microscope (TEM) images of a micelle formed by an ion pair formed by hydrogen bonding between caprylic acid and arginine in Example 1 and a nanovesicle formed by containing betulin inside the micelle structure in Example 2.

[0047] Figure 8 is a diagram comparing the results of measuring 1H-NMR for caprylic acid, arginine, and betulin with the results of measuring 1H-NMR for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0048] Figure 9 is a diagram comparing the results of FT-IR measurements for caprylic acid, arginine, and betulin with the results of FT-IR measurements for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0049] Figure 10 is a diagram comparing the results of Differential Scanning Calorimetry (DSC) measurements for betulin with the results of Differential Scanning Calorimetry (DSC) measurements for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin being included inside the micelle structure in Example 2.

[0050] FIG. 11 is a diagram showing the antioxidant activity evaluated through DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis for a cosmetic composition for improving photoaging and thermal aging, which includes arginine, betulin, and nanovesicles formed by including betulin inside the micelle structure of Example 2.

[0051] Figure 12 is a diagram showing the evaluation of inhibition of the inflammation-inducing factor MCP-1 by ultraviolet rays (Ultraviolet B, UVB).

[0052] Figure 13 is a figure showing the evaluation of inhibition of the inflammatory factor IL4 by ultraviolet rays (Ultraviolet B, UVB).

[0053] Figure 14 is a figure showing the evaluation of inhibition of the inflammatory factor IL8 by ultraviolet rays (Ultraviolet B, UVB).

[0054] In the present invention, the attached drawings may be illustrated with exaggerated expressions to distinguish it from the prior art, ensure clarity, and facilitate the understanding of the technology. Furthermore, the terms and words described below are defined considering their functions in the present invention; since these may vary depending on the intentions or conventions of the user or operator, the definitions of such terms and words should be based on the technical content throughout this specification. Moreover, terms and words used in this specification and claims are not limited to their ordinary or dictionary meanings; rather, based on the premise that the inventor may appropriately define the concepts of terms and words to best describe their invention, they should be interpreted in a meaning and concept consistent with the technical spirit of the present invention. Meanwhile, embodiments or experimental examples are merely exemplary details of the components presented in the claims of the present invention and do not limit the scope of rights of the present invention; the scope of rights should be interpreted based on the technical spirit throughout the specification of the present invention. In addition, the embodiments or experimental examples described in the specification of the present invention are merely the most preferred embodiments or experimental examples of the present invention and do not represent all of the technical ideas of the present invention; therefore, it should be understood that various equivalents and modifications that can replace them may be possible or exist at the time of filing the application for the present invention.

[0055] Furthermore, prior to describing the present invention, it should be noted that matters not necessary to reveal the gist of the invention—namely, known configurations that a person skilled in the art with ordinary knowledge could obviously add—have not been explained or described in detail.

[0056] Hereinafter, preferred embodiments and experimental examples of the present invention will be described.

[0057] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be composed of one or more micelles formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or micelles formed by hydrogen bonding between arginine and one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms.

[0058] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, arginine is known to be effective for anti-inflammation through the scavenging of reactive oxygen species based on its antioxidant capacity; however, due to the high pH that appears when dissolved in water, it must be used in combination with a neutralizing agent, which limits its use to only specific applications. Furthermore, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, arginine is almost insoluble in oils or alcohols and hardly dissolves in the general solvents constituting the cosmetic composition. Consequently, it is difficult to apply high concentrations to the cosmetic composition, which limits the improvement of photoaging and thermal aging. Additionally, there are limitations in the alleviation of inflammation caused by photoaging or thermal aging and the resulting aging improvement effects, as well as limitations in skin improvement effects due to the difficulty of applying arginine at high concentrations to the cosmetic composition. However, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, arginine can form an ion pair by hydrogen bonding with one or more of saturated fatty acids having 8 to 12 carbon atoms, and such an ion pair can be formed by hydrogen bonding between the guanidyl group of arginine, which causes basicity in the solvent, and the carboxyl group of the saturated fatty acid having 8 to 12 carbon atoms. In addition, the ion pair can be formed by hydrogen bonding between the amine group of arginine, which causes basicity in the solvent, and the carbonyl group of a triglyceride containing one or more of the saturated fatty acids having 8 to 12 carbon atoms.Furthermore, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles may be formed by ion pairs formed by hydrogen bonding between arginine and one or more of saturated fatty acids having 8 to 12 carbon atoms. Additionally, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles may be formed by ion pairs formed by hydrogen bonding between arginine and one or more of triglycerides containing 8 to 12 carbon atoms. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the solubility of arginine in water can be improved by 30-50% through the formation of the micelles. More specifically, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, when micelles are formed by ion pairs formed by hydrogen bonding between caprylic acid, a saturated fatty acid having 8 carbon atoms, and arginine, it can be confirmed that the solubility of arginine in water is improved from 14.87 g / 100 mL (20°C) to 20 g / 100 mL (20°C).

[0059] In addition, the cosmetic composition for improving photoaging and thermal aging according to the present invention may have neutral characteristics due to a micelle structure formed by an ion pair formed by hydrogen bonding between one or more of saturated fatty acids having 8 to 12 carbon atoms and arginine. Specifically, the guanidyl group of arginine, which exhibits basicity, is masked by hydrogen bonding, and arginine, which exhibits basicity when dissolved in water alone with a pH value of 11-13, has neutral characteristics, so the cosmetic composition for improving photoaging and thermal aging according to the present invention may have a pH value of 6.5-7.5.

[0060] In addition, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, arginine can form an ion pair by hydrogen bonding with one or more triglycerides containing one or more of saturated fatty acids having 8 to 12 carbon atoms, and such ion pair can be formed by hydrogen bonding between the guanidyl group of arginine, which causes basicity in a solvent, and the carbonyl group of the triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms. In addition, the above ion pair may be formed by hydrogen bonding between the amine group of arginine, which causes basicity in a solvent, and the carbonyl group of a triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms. Furthermore, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles may be formed by ion pairs formed by hydrogen bonding between arginine and one or more of triglycerides containing one or more of saturated fatty acids having 8 to 12 carbon atoms. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the solubility of arginine in water can be improved by 30-50% through the formation of the above micelles.In addition, the cosmetic composition for improving photoaging and thermal aging according to the present invention may have neutral characteristics due to a micelle structure formed by ion pairs formed by hydrogen bonding between arginine and one or more triglycerides containing one or more of saturated fatty acids having 8 to 12 carbon atoms. Specifically, the guanidyl group of arginine, which exhibits basicity, is masked by hydrogen bonding, and arginine, which has a pH value of 11-13 and exhibits basicity when dissolved in water alone, has neutral characteristics, so the cosmetic composition for improving photoaging and thermal aging according to the present invention may have a pH value of 6.5-7.5.

[0061] Furthermore, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, in the case of saturated fatty acids with fewer than 8 carbon atoms or fatty acids with more than 12 carbon atoms, problems may occur in the formation of micelle structures formed by ion pairs through hydrogen bonding with arginine, and problems may occur in the formation of nanovesicle structures loaded with insoluble components. In addition, even when unsaturated fatty acids are used in the cosmetic composition for improving photoaging and thermal aging according to the present invention, problems may occur in the formation of micelle structures formed by ion pairs through hydrogen bonding with arginine, and problems may occur in the formation of nanovesicle structures loaded with insoluble components. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, a single type of saturated fatty acid with 8 to 12 carbon atoms may be used, or a mixture of two or more types of saturated fatty acids may be used. In addition, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, the triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms may include a single type of saturated fatty acid or may include a mixture of two or more types of saturated fatty acids. Furthermore, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, the triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms may use a single type of triglyceride or may use a mixture of two or more types of triglycerides.

[0062] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, a triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms or 8 to 12 carbon atoms has the characteristic of providing moisture and flexibility to the skin, but due to its utility characteristics, the triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms or 8 to 12 carbon atoms has limitations in application to water-based cosmetic formulations (skin toners, ampoules, essences, etc.) that provide aesthetic pleasure. Due to these limitations, problems may arise in the occurrence of the antioxidant effect, anti-inflammatory effect, photoaging reduction effect, or thermal aging reduction effect of the triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms or 8 to 12 carbon atoms. Furthermore, in the case of triglycerides containing one or more of carbon-8 to carbon-12 saturated fatty acids or carbon-8 to carbon-12 saturated fatty acids, they can be sufficiently applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.) that provide aesthetic pleasure only when a separate surfactant is used, but problems may arise due to various side effects caused by the use of surfactants.However, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles may be formed by ion pairs formed by hydrogen bonding between arginine and one or more of saturated fatty acids having C8 to C12 or triglycerides containing one or more of saturated fatty acids having C8 to C12, and through the formation of said micelles, the water-insoluble saturated fatty acids having C8 to C12 or triglycerides containing one or more of saturated fatty acids having C8 to C12 may have a solubility in water of 15-25 g / 100 mL (at 20°C), and may be sufficiently applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.) that provide aesthetic pleasure, and sufficient antioxidant effect, anti-inflammatory effect, photoaging reduction effect, or thermal aging reduction It can have an effect. More specifically, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, when micelles are formed by ion pairs formed by hydrogen bonding between caprylic acid, a saturated fatty acid having 8 carbon atoms, and arginine, caprylic acid, which is insoluble in water, can have a solubility in water of 20g / 100mL (20℃).

[0063] Figure 1 is a diagram showing the formation of ion pairs by two strong hydrogen bonds between the guanidyl group of arginine and the carboxyl group of caprylic acid in the cosmetic composition for improving photoaging and thermal aging according to the present invention, and the formation of micelles by self-assembly.

[0064] According to FIG. 1, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, an ion pair formed by two strong hydrogen bonds can be formed between two nitrogen atoms of the guanidyl group of arginine and two oxygen atoms of the carboxyl group of caprylic acid. That is, since the guanidyl group of arginine has two positively charged bonding arms (cations) and the carboxyl group of caprylic acid has two negatively charged bonding arms (anions) having lone pairs of electrons, hydrogen bonds can be formed by the two positively charged bonding arms (cations) and the two negatively charged bonding arms (anions), and an ion pair can be formed. A pair of ion pairs can be formed between arginine and caprylic acid in a molar ratio range of 1:3 to 3:1, and preferably, a pair of ion pairs can be formed between arginine and caprylic acid in a molar ratio of 1:1. Since the pair of ion pairs formed through this process has the structure of a surfactant, micelles, which are spherical particles, can be formed by self-assembly. Micelles, which are spherical particles, can be formed only within the specific concentration conditions and molar ratio range described below, and if the specific concentration conditions and molar ratio range are exceeded, a problem may arise in which a sponge structure, a liquid crystal structure, or an amorphous structure is formed instead of a micelle structure.

[0065] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the micelles may be characterized by having an average diameter of 1-10 nm.

[0066] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the micelles may be formed as spherical particles by using an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms as a hydrophobic tail. The micelles may have various diameters, preferably having an average diameter of 1 to 10 nm. More preferably, the micelles may have an average diameter of 3 to 7 nm, and most preferably, an average diameter of 4.9 nm. If the micelles have a diameter of less than 1 nm, a problem may arise in that spherical particles themselves are not formed, with the amine group or carboxyl group of arginine as the hydrophilic head and the carbon chain of a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms as the tail. When the micelles have a diameter greater than 10 nm, the spherical particle structure formed with an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms as a tail may collapse, resulting in reduced stability.

[0067] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be characterized by additionally including a sparingly soluble component inside the micelle structure to form nanovesicles.

[0068] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, a poorly soluble component is additionally included within the micelle structure to form a nanovesicle, thereby making it possible to dissolve the poorly soluble component, which is insoluble in water and compatible only with specific oils, in water. This allows for sufficient application in water-based cosmetic formulations (skin toners, ampoules, essences, etc.) that provide aesthetic pleasure. Furthermore, it has the characteristic of solving the problem of poorly soluble components compatible only with specific oils being difficult to deliver to the skin through the stratum corneum. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the poorly soluble component refers to a substance that exists unstably because it is not sufficiently dissolved in either water or oil. It can have beneficial effects on the skin, such as antioxidant action, anti-inflammatory action, wrinkle improvement, skin whitening, and skin moisturization, and can have beneficial effects for improving photoaging and thermal aging. As a result, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, the nano vesicles can simultaneously have the characteristic of increasing the solubility of insoluble components in polar water and the characteristic of being easily delivered to the skin through non-polar keratin.

[0069] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, spherical micelle particles formed by using an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms as a hydrophobic tail, or nano vesicles formed by adding and expanding a sparingly soluble component inside spherical micelle particles formed by using an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms as a hydrophobic tail, Since it can be formed within the pH range of 4 to 7, which is the general pH range of cosmetics or skin, the cosmetic composition for improving photoaging and thermal aging according to the present invention has the characteristic of being universally applicable to various cosmetics and various skin types.

[0070] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the nanovesicle may be characterized by having an average diameter of 100-500 nm.

[0071] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the nanovesicle may be formed by adding and expanding a sparingly soluble component inside a spherical micelle particle formed by using an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms as a hydrophobic tail. The nanovesicle may have various diameters, preferably having an average diameter of 100 to 500 nm. More preferably, the nanovesicle may have an average diameter of 300 to 500 nm, and most preferably, an average diameter of 471 nm. When nanovesicles have a diameter of less than 100 nm, the spherical micelle particles formed by having an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid with 8 to 12 carbon atoms or a saturated fatty acid with 8 to 12 carbon atoms as a hydrophobic tail are not sufficiently added to the inside, which may cause problems with the beneficial effects on the skin, such as antioxidant action, anti-inflammatory action, wrinkle improvement, skin whitening, and skin moisturization, as well as the beneficial effects on improving photoaging and thermal aging caused by the insoluble components.When nanovesicles have a diameter greater than 500 nm, a problem may arise in which the nanovesicle structure formed by adding and expanding an insoluble component inside a spherical micelle particle, formed with an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of a triglyceride containing one or more of a saturated fatty acid with 8 to 12 carbon atoms or a saturated fatty acid with 8 to 12 carbon atoms as a tail, collapses and stability decreases.

[0072] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be characterized in that the insoluble component includes one or more of betulin or quercetin.

[0073] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, betulin is a representative indicator component with antioxidant effects found in birch sap along with betulinic acid, and as a lupane-type pentacyclic triterpenoid saponin, it has useful effects in antioxidant and anti-inflammatory action. In particular, due to its structural similarity to steroids, betulin inhibits the activity of the cyclooxygenase-2 (COX-2) enzyme in macrophages, thereby having excellent anti-inflammatory effects. However, betulin is a poorly soluble substance that is not soluble in water and is only soluble in specific organic solvents such as methanol, ethanol, and 1-propanol, as well as selective oils and fatty acids. Consequently, it is difficult to apply it in high concentrations within cosmetics, and there was a problem in that it could not be applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.). However, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, when betulin is loaded inside micelles formed by an ion pair between arginine and a triglyceride containing one or more of saturated fatty acids having C8 to C12 or saturated fatty acids having C8 to C12, it has the characteristic of being stably applicable to water-based cosmetic formulations (skin toners, ampoules, essences, etc.) without direct contact with water. Generally, when betulin is directly applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.), it was possible to apply it at a low content level of 0.002g / 100mL or less; however, when betulin is loaded inside micelles in the cosmetic composition for improving photoaging and thermal aging according to the present invention, a maximum of 0.It can be applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.) at a content level of 5g / 100mL, and has an anti-inflammatory effect by inhibiting the activity of cyclooxygenase-2 (COX-2) enzyme, and can have an enhanced effect in improving photoaging and thermal aging. In addition, there was a problem with betulin being difficult to deliver to the skin through the stratum corneum. However, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, when betulin is loaded inside micelles formed by an ion pair between arginine and a triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms or 8 to 12 carbon atoms, it becomes easier to deliver to the skin through the stratum corneum, and an effect of excellent anti-inflammatory action can occur.

[0074] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, quercetin is a plant-derived flavonolosa and is characterized by having excellent antioxidant effects. In particular, quercetin can have a skin soothing effect based on its antioxidant efficacy and an anti-inflammatory effect through the inhibition of inflammatory factor cytokines. Quercetin has the characteristic of being insoluble in water and is compatible only with specific oils. That is, quercetin is a poorly soluble substance that does not dissolve in water and is only soluble in selective oils and fatty acids. As such, it is difficult to apply it in high concentrations in cosmetics, and there was a problem that it could not be applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.). However, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, when quercetin is loaded inside a micelle formed by an ion pair with arginine and a triglyceride containing one or more of a saturated fatty acid having 8 to 12 carbon atoms or a saturated fatty acid having 8 to 12 carbon atoms, it has the characteristic of being able to be stably applied to water-based cosmetic formulations (skin toner, ampoule, essence, etc.) without direct contact with water. Furthermore, quercetin is loaded onto micelles formed by ion pairs between arginine and triglycerides containing one or more of C8–C12 saturated fatty acids, thereby forming nano-vesicles, and there is a characteristic that quercetin, a poorly soluble substance, can stably exist within water-based cosmetic formulations (skin toners, ampoules, essences, etc.). Generally, when quercetin is directly applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.), 0.Although it was possible to apply it at a low content level of 0.02 g / 100 mL or less, when quercetin is loaded inside micelles in the cosmetic composition for improving photoaging and thermal aging according to the present invention, it can be applied to water-based cosmetic formulations (skin toners, ampoules, essences, etc.) at a maximum content level of 2 g / 100 mL. It is characterized by having a skin soothing effect based on antioxidant efficacy and an anti-inflammatory effect through the inhibition of inflammatory factor cytokines, and having an enhanced effect in improving photoaging and thermal aging. In addition, there was a problem that quercetin was difficult to deliver to the skin through the stratum corneum. However, in the cosmetic composition for improving photoaging and thermal aging according to the present invention, when quercetin is loaded into micelles formed by ion pairs with arginine and triglycerides containing one or more of saturated fatty acids having 8 to 12 carbon atoms or 8 to 12 carbon atoms, it is easily delivered to the skin through the stratum corneum, and a skin soothing effect based on excellent antioxidant efficacy and an anti-inflammatory effect through the inhibition of inflammatory factor cytokines can occur.

[0075] In other words, when betulin or quercetin is loaded into an ion pair with arginine and a triglyceride containing one or more of saturated fatty acids having 8 to 12 carbon atoms or 8 to 12 carbon atoms, the betulin or quercetin dissolves in polar water and is easily delivered to the skin through the non-polar keratin, and can have the effects of removing reactive oxygen species (ROS) induced by heat or light, skin soothing effects based on antioxidant efficacy, anti-inflammatory effects through inhibition of inflammatory factor cytokines, anti-inflammatory effects through inhibition of cyclooxygenase-2 (COX-2) enzyme activity, or alleviation of aging symptoms. Furthermore, water solubility and stability are significantly enhanced without the addition of separate surfactants, micelles with a hydrophilic head and a hydrophobic tail structure are formed by ion pairs between arginine and triglycerides containing one or more of C8-C12 saturated fatty acids or C8-C12 saturated fatty acids, and nano vesicles are formed by loading betulin or quercetin; thus, since the poorly soluble substances betulin or quercetin can dissolve in polar water and be easily delivered to the skin through the non-polar stratum corneum, the cosmetic composition for improving photoaging and thermal aging according to the present invention can provide a refreshing aesthetic pleasure without the irritation and stickiness derived from surfactants, minimize skin irritation caused by surfactants, and photo Alternatively, it has features suitable for skin sensitized by heat or naturally sensitive skin.Furthermore, by composing a cosmetic composition for improving photoaging and thermal aging without a separate oil composition, it is suitable for application to acne-prone and troubled skin that is susceptible to oil compositions, reduces skin surface shielding that may occur due to a separate oil composition, and reduces sebum secretion and inflammation caused by exposure to light or heat, thereby minimizing the proliferation of anaerobic bacteria, including acne-causing bacteria (C. acnes).

[0076] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the insoluble component may include betulin alone, quercetin alone, or a mixture of betulin and quercetin. In the case of a mixture of betulin and quercetin, there is no particular limitation on the ratio, and the ratio of the mixture of betulin and quercetin may be adjusted according to skin conditions and skin state.

[0077] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the saturated fatty acid having 8 to 12 carbon atoms may be characterized by having a carboxyl group only at the 1st carbon and having a linear structure rather than a branched structure.

[0078] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, if the saturated fatty acid having 8 to 12 carbon atoms has a branched structure, it is difficult to form an ion pair with a triglyceride containing one or more of the saturated fatty acid having 8 to 12 carbon atoms having a branched structure or the saturated fatty acid having 8 to 12 carbon atoms having a branched structure, and difficulties may arise in forming a micelle structure with a hydrophilic head and a hydrophobic tail. Furthermore, since there are difficulties in forming a micelle structure, betulin or quercetin cannot be loaded into the micelle structure, which may cause problems in the formation of nano vesicles.

[0079] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the saturated fatty acid having a linear structure with 8 to 12 carbon atoms may be characterized as being caprylic acid, capric acid, or lauric acid.

[0080] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, if the saturated fatty acid having a linear structure with 8 to 12 carbon atoms includes a saturated fatty acid other than caprylic acid, capric acid, or lauric acid, the efficiency of forming an ion pair with arginine may decrease, and the efficiency of forming a micelle structure with a hydrophilic head and a hydrophobic tail, as well as the efficiency of forming a nano vesicle structure by loading with betulin or quercetin, may decrease.

[0081] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be characterized by additionally including a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at carbon 1 and carbon 2, and not having a branched structure.

[0082] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at carbon 1 and carbon 2 and not having a branched structure is additionally included, thereby improving the structural stability of an ion pair formed with arginine and one or more of caprylic acid, capric acid, or lauric acid, and improving the structural stability of an ion pair formed with one or more of triglycerides containing arginine and one or more of caprylic acid, capric acid, or lauric acid. The cosmetic composition for improving photoaging and thermal aging according to the present invention may have the characteristic of improving the structural stability of micelles with hydrophilic heads and hydrophobic tails and nanovesicles by loading a sparingly soluble component such as betulin or quercetin through the addition of a linear diol having 6 to 12 carbons that has hydroxyl groups at carbons 1 and 2 and does not have a branched structure.If the cosmetic composition for improving photoaging and thermal aging according to the present invention does not additionally include a linear diol having 6 to 12 carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure, a problem may arise in which the ion pair structural stability, micelle structural stability, and nano vesicle structural stability with arginine and a triglyceride containing one or more of caprylic acid, capric acid, or lauric acid may be reduced.In the cosmetic composition for improving photoaging and thermal aging according to the present invention, a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms and not having a branched structure is additionally included, so that after a hydrogen bond is formed between the carboxyl group of caprylic acid, capric acid, or lauric acid, or the carbonyl group of a triglyceride containing one or more of caprylic acid, capric acid, or lauric acid, and the hydrogen of the linear diol having 6 to 12 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms and not having a branched structure, the carboxyl group of caprylic acid, capric acid, or lauric acid Micelles can be stably formed by ion pairs formed by hydrogen bonds between the carbonyl group of a triglyceride containing one or more of a carboxyl group, caprylic acid, capric acid, or lauric acid, and the guanidyl group of arginine.In the process of forming a micelle structure, which is a spherical particle having a hydrophilic head with an amine group or a carboxyl group of arginine and a carbon chain of caprylic acid, capric acid, or lauric acid, or a carbon chain of a triglyceride containing one or more of caprylic acid, capric acid, or lauric acid as a hydrophobic tail, a linear diol having 6 to 12 carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure, used as a dispersant, can perform the role of increasing the stability of the micelle structure because it has a similar structure of a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration. Furthermore, the stability of the nano vesicle structure by loading with a sparingly soluble component such as betulin or quercetin can also be improved by a linear diol having 6 to 12 carbon atoms that has a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration, has hydroxyl groups on carbons 1 and 2, and does not have a branched structure.

[0083] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the linear diol may be characterized as being 1,2-hexanediol.

[0084] The cosmetic composition for improving photoaging and thermal aging according to the present invention may have the characteristic of having improved ion pair structural stability with arginine and triglycerides containing one or more of caprylic acid, capric acid, lauric acid, or caprylic acid, capric acid, or lauric acid, improved micelle structural stability of hydrophilic head and hydrophobic tail, and improved nano vesicle structural stability through loading of insoluble components such as betulin or quercetin.By additionally including 1,2-hexanediol in the cosmetic composition for improving photoaging and thermal aging of the present invention, hydrogen bonds are formed between the carboxyl group of caprylic acid, capric acid, or lauric acid, or the carbonyl group of a triglyceride containing one or more of caprylic acid, capric acid, or lauric acid, and the hydrogen of 1,2-hexanediol. Micelles can be stably formed by ion pairs formed by hydrogen bonds between guanidyl groups of arginine.In the process of forming a micelle structure, which is a spherical particle having an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of caprylic acid, capric acid, or lauric acid, or a carbon chain of a triglyceride containing one or more of caprylic acid, capric acid, or lauric acid as a hydrophobic tail, 1,2-hexanediol used as a dispersant has a structure similar to a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration, and thus can play a role in increasing the stability of the micelle structure. Furthermore, the stability of the nano vesicle structure by loading with poorly soluble components such as betulin or quercetin can also be improved by 1,2-hexanediol, which has a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration.

[0085] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be characterized by including micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, wherein the insoluble component is betulin, and the arginine is 0.1 to 20 parts by weight, the caprylic acid is 0.098 to 20 parts by weight, the betulin is 0.002 to 0.5 parts by weight, and the water is 59.5 to 99.8 parts by weight.

[0086] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine are included, and when the insoluble component is betulin, arginine may be included in an amount of 0.1 to 20 parts by weight. If arginine is included in an amount less than 0.1 parts by weight, sufficient effects for antioxidant and anti-aging cannot be expected, and if it is included in an amount greater than 20 parts by weight, ion pairs formed by hydrogen bonding between the guanidyl group of arginine and the carboxyl group of caprylic acid are not properly formed, which may cause a problem of insufficient dissolution in water. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine are included, and when the insoluble component is betulin, the caprylic acid may be included in an amount of 0.098 to 20 parts by weight. If the caprylic acid is included in an amount less than 0.098 parts by weight, a problem may occur in the formation of nano vesicles, and thus the antioxidant and anti-inflammatory effects of betulin cannot be expected. If the caprylic acid is included in an amount greater than 20 parts by weight, a problem may occur in that the ion pair formed by hydrogen bonding between the carboxyl group of the caprylic acid and the guanidyl group of the arginine is not properly formed, and thus it may not dissolve sufficiently in water.In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine are included, and when the insoluble component is betulin, the betulin may be included in an amount of 0.002 to 0.5 parts by weight; if the betulin is included in an amount less than 0.002 parts by weight, antioxidant and anti-inflammatory effects by betulin cannot be expected, and if it is included in an amount greater than 0.5 parts by weight, betulin does not dissolve properly in water, and betulin that is not supported on nano vesicles precipitates, or the unsupported betulin forms ion pairs between arginine and caprylic acid, which have relatively higher compatibility with water than water. A problem may arise in which weak intermolecular bonds are induced in the nano vesicles, thereby impairing their stability. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine are included, and when the insoluble component is betulin, the water may be included in an amount of 59.5 to 99.8 parts by weight. If the water is included in an amount less than 59.5 parts by weight, a problem may arise regarding the stability of the nano vesicles loaded with betulin, and if it is included in an amount exceeding 99.8 parts by weight, the full antioxidant and anti-inflammatory effects of betulin cannot be expected, and economic feasibility may also be compromised.

[0087] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be characterized by including micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, wherein the insoluble component is quercetin, and the composition may include 0.1 to 20 parts by weight of the arginine, 0.098 to 20 parts by weight of the caprylic acid, 0.002 to 2 parts by weight of the quercetin, and 58 to 99.8 parts by weight of the water.

[0088] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine are included, and when the insoluble component is quercetin, arginine may be included in an amount of 0.1 to 20 parts by weight. If arginine is included in an amount less than 0.1 parts by weight, sufficient effects for antioxidant and anti-aging cannot be expected, and if it is included in an amount greater than 20 parts by weight, ion pairs formed by hydrogen bonding between the guanidyl group of arginine and the carboxyl group of caprylic acid are not properly formed, which may cause a problem of insufficient dissolution in water. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine are included, and when the insoluble component is quercetin, the caprylic acid may be included in an amount of 0.098 to 20 parts by weight. If the caprylic acid is included in an amount less than 0.098 parts by weight, problems with nano-vesicle formation occur, making it impossible to expect skin soothing and anti-inflammatory effects by quercetin; if it is included in an amount exceeding 20 parts by weight, ion pairs formed by hydrogen bonding between the carboxyl group of caprylic acid and the guanidyl group of arginine are not properly formed, which may result in a problem of insufficient dissolution in water. there is.In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs resulting from hydrogen bonding between the caprylic acid and the arginine are included, and when the insoluble component is quercetin, the quercetin may be included in an amount of 0.002 to 2 parts by weight; if the quercetin is included in an amount less than 0.002 parts by weight, the skin soothing and anti-inflammatory effects by the quercetin cannot be expected, and if it is included in an amount exceeding 2 parts by weight, the quercetin does not dissolve properly in water, and quercetin that is not supported on nano vesicles precipitates, or the unsupported quercetin interacts with arginine, which has relatively higher compatibility with water, in water. A problem may arise in which weak intermolecular bonding is induced in the ion pairs between caprylic acids, thereby impairing the stability of the nano vesicles. In the cosmetic composition for improving photoaging and thermal aging according to the present invention, micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine are included, and when the insoluble component is quercetin, the water may be included in an amount of 58 to 99.8 parts by weight. If the water is included in an amount less than 58 parts by weight, a problem may arise regarding the stability of the nano vesicles loaded with quercetin, and if it is included in an amount exceeding 99.8 parts by weight, the substantial skin soothing and anti-inflammatory effects by quercetin cannot be expected, and economic issues may also arise.

[0089] The cosmetic composition for improving photoaging and thermal aging according to the present invention may be characterized by including micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the caprylic acid and the arginine having a molar ratio of 1:3 to 3:1.

[0090] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, if the molar ratio of the caprylic acid and the arginine is exceeded by 1:3 and the arginine is excessively added, there may be a problem in which the arginine that cannot form an ion pair remains and precipitation occurs, and if the molar ratio of the caprylic acid and the arginine is exceeded by 3:1 and the caprylic acid is excessively added, there may be a problem in which the caprylic acid that cannot form an ion pair separates into phases in the upper layer of water. Furthermore, the cosmetic composition for improving photoaging and thermal aging according to the present invention includes micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the caprylic acid and the arginine may have the most stable ion pair structure, micelle structure, and nano vesicle structure when they have a 1:1 molar ratio.

[0091] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, the triglyceride containing one or more of the saturated fatty acids having 8 to 12 carbon atoms may be characterized as being glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride.

[0092] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, if the triglyceride containing one or more of the saturated fatty acids having 8 to 12 carbon atoms includes a triglyceride other than glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride, the efficiency of forming ion pairs with arginine may decrease, and the efficiency of forming micelle structures with hydrophilic heads and hydrophobic tails and the efficiency of forming nano vesicle structures by loading betulin or quercetin may decrease.

[0093] The cosmetic composition for improving photoaging and thermal aging according to the present invention, comprising glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride, may be characterized by additionally including a linear diol having 6 to 12 carbon atoms that has hydroxyl groups at carbons 1 and 2 and does not have a branched structure.

[0094] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, which comprises glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride, a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at carbons 1 and 2, and not having a branched structure is additionally included, thereby improving the structural stability of the ion pair between arginine and glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride, and the hydrophilic head and hydrophobic It may have the characteristic of improved structural stability of the micelles of the tail and structural stability of the nano vesicles through loading of insoluble components such as betulin or quercetin. If the cosmetic composition for improving photoaging and thermal aging according to the present invention does not additionally include a linear diol having 6 to 12 carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure, a problem may arise in which the ion pair structural stability, micelle structural stability, and nano vesicle structural stability between arginine and glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride are reduced.In the cosmetic composition for improving photoaging and thermal aging according to the present invention, a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms and not having a branched structure is additionally included, wherein after the carbonyl group of glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride forms a hydrogen bond with the hydrogen of the linear diol having 6 to 12 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms and not having a branched structure, the carbonyl group of glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride forms a hydrogen bond with the hydrogen of the linear diol having 6 to 12 carbon atoms and not having a branched structure. Micelles can be stably formed by ion pairs formed by hydrogen bonds between the carbonyl group of trioctanoate or caprylic / capric triglyceride and the guanidyl group of arginine.In the process of forming a micelle structure, which is a spherical particle having an amine group or a carboxyl group of arginine as a hydrophilic head and a carbon chain of glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride as a hydrophobic tail, a linear diol having 6 to 12 carbon atoms that has hydroxyl groups at carbon 1 and carbon 2 and does not have a branched structure, used as a dispersant, can perform the role of increasing the stability of the micelle structure because it has a similar structure of hydrophilic hydroxyl group composition and hydrophobic carbon skeleton composition. Furthermore, the stability of the nano vesicle structure by loading with a sparingly soluble component such as betulin or quercetin can also be improved by a linear diol having 6 to 12 carbon atoms that has a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration, has hydroxyl groups on carbons 1 and 2, and does not have a branched structure.

[0095] In the cosmetic composition for improving photoaging and thermal aging according to the present invention, which includes glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride, the linear diol may be characterized as being 1,2-hexanediol.

[0096] By additionally including 1,2-hexanediol in the cosmetic composition for improving photoaging and thermal aging according to the present invention, which comprises glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride, the ion pair structural stability between arginine and glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride is improved, and the micelle structural stability of the hydrophilic head and hydrophobic tail and betulin or It may have the characteristic of improved structural stability of nano vesicles due to loading of insoluble components such as quercetin.By additionally including 1,2-hexanediol in the cosmetic composition for improving photoaging and thermal aging according to the present invention, hydrogen bonds are formed between the carbonyl group of glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride and the hydrogen of 1,2-hexanediol, and subsequently, hydrogen bonds are formed between the carbonyl group of glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride and the guanidyl group of arginine. Micelles can be stably formed by ion pairs formed by bonds. In the process of forming a micelle structure, which is a spherical particle with an amine group or carboxyl group of arginine as a hydrophilic head and a carbon chain of glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride as a hydrophobic tail, 1,2-hexanediol, which is used as a dispersant, has a similar structure with a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration, and thus can play a role in increasing the stability of the micelle structure.Furthermore, the stability of the nano vesicle structure by loading with poorly soluble components such as betulin or quercetin can also be improved by 1,2-hexanediol, which has a hydrophilic hydroxyl group configuration and a hydrophobic carbon skeleton configuration.

[0097] FIG. 2 is a flowchart illustrating a method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention.

[0098] According to FIG. 2, the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention comprises the steps of: adding 0.1 to 20 parts by weight of arginine to 59.5 to 99.8 parts by weight of water and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; and adding 0.098 to 20 parts by weight of caprylic acid to the first solution while heating to 50°C to 90°C and stirring with a homodisper at 2,000 rpm to 4,000 rpm for 10 to 30 minutes to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine. It is possible.

[0099] In the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, 0.1 to 20 parts by weight of arginine may be added to 59.5 to 99.8 parts by weight of water. If the arginine is included in an amount less than 0.1 parts by weight, sufficient effects for antioxidant and anti-aging effects cannot be expected, and if it is included in an amount greater than 20 parts by weight, a problem may occur in which an ion pair formed by a hydrogen bond between the guanidyl group of arginine and the carboxyl group of caprylic acid is not properly formed, and thus it does not dissolve sufficiently in water. Furthermore, in the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, 0.1 to 20 parts by weight of arginine may be added to 59.5 to 99.8 parts by weight of water. If the amount of water is less than 59.5 parts by weight, a problem may arise regarding the stability of micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine. If the amount of water is greater than 99.8 parts by weight, a problem may arise where the antioxidant and anti-aging effects of arginine cannot be expected. The temperature of the step in which the first solution in which arginine is dissolved in water is produced can be carried out at room temperature, but is not limited thereto and has the characteristic of being able to be carried out under various temperature conditions.However, in the method for manufacturing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step in which the first solution in which arginine is dissolved in water is generated is too low, then in the subsequent step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, too much time is required to heat the first solution to 50°C - 90°C while adding the caprylic acid due to the low temperature of the first solution, and problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine. Furthermore, if the temperature of the step in which the first solution in which arginine is dissolved in water is generated is too high in the method for manufacturing a cosmetic composition for improving photoaging and thermal aging according to the present invention, then the caprylic acid and the arginine In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, water may evaporate due to the high temperature of the first solution, and a problem may arise where arginine is reprecipitated from the first solution. Furthermore, if the temperature of the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine is less than 50°C, a problem may arise in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if it exceeds 90°C, a problem may arise where the off-flavor of the caprylic acid becomes severe, making it unsuitable for application in cosmetic compositions, and a problem may arise in the stability of the micelles due to water evaporation.In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, stirring may be performed using a homodisper, but is not limited thereto. If the stirring time is less than 10 minutes in the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if the stirring time exceeds 30 minutes, problems may occur in energy efficiency. In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, if the stirring speed is less than 2,000 rpm, problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if the stirring speed exceeds 4,000 rpm, problems may occur in energy efficiency.

[0100] FIG. 3 is another flowchart illustrating a method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention.

[0101] According to FIG. 3, the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention comprises the steps of: adding 0.1 to 20 parts by weight of arginine to 59.5 to 99.8 parts by weight of water and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; adding 0.002 to 0.5 parts by weight of betulin and 0.098 to 20 parts by weight of caprylic acid to 50℃ to 90℃ and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a seconda solution in which betulin is dissolved in caprylic acid; and The method may be configured to include the step of adding the seconda solution to the first solution, heating it to 50°C - 90°C, stirring it at 2,000 rpm - 4,000 rpm for 10 - 30 minutes using a homodisper, thereby forming micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the step of forming nanovesicles by including the betulin inside the micelle structure.

[0102] In the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, the temperature of the step in which a first solution in which arginine is dissolved in water is generated can be carried out at room temperature, but is not limited thereto and has the characteristic that it can be carried out under various temperature conditions. However, in the method for manufacturing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step in which the first solution in which arginine is dissolved in water is generated is too low, then in the subsequent step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, too much time is required to heat the first solution to 50°C - 90°C when the seconda solution is added to the first solution due to the low temperature of the first solution, and a problem may occur in which betulin dissolved in the seconda solution reprecipitates. Furthermore, if the temperature of the step in which the first solution in which arginine is dissolved in water is generated is too high, then in the subsequent step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine In the step where micelles are formed, water may evaporate due to the high temperature of the first solution, and a problem may occur in which arginine is reprecipitated from the first solution. In the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step in which the seconda solution in which betulin is dissolved in caprylic acid is produced is less than 50°C, a problem may occur in which betulin is not sufficiently dissolved in caprylic acid, and if it exceeds 90°C, a problem may occur in which the off-flavor of caprylic acid becomes severe, making it unsuitable for application in a cosmetic composition.In the step where the second solution of 2a is produced, a homodisper may be used for stirring, but it is not limited to this, and various stirring methods may be utilized. If the stirring time is less than 15 minutes in the step where the second solution of 2a, in which betulin is dissolved in caprylic acid, is produced, a problem may occur where betulin is not sufficiently dissolved in caprylic acid and residue remains, and if the stirring time exceeds 25 minutes, a problem may occur with energy efficiency. If the stirring speed is less than 1,500 rpm in the step where the second solution of 2a, in which betulin is dissolved in caprylic acid, is produced, a problem may occur where betulin is not sufficiently dissolved in caprylic acid and residue remains, and if the stirring speed exceeds 2,500 rpm, a problem may occur with energy efficiency. Furthermore, if the temperature of the step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine is less than 50°C, problems may arise in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine; if the temperature exceeds 90°C, the off-flavor of the caprylic acid may become severe, making it unsuitable for application in cosmetic compositions, and water may evaporate, which may cause problems with the stability of the micelles. A homodisper may be used for stirring during the step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, but is not limited thereto.In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, if the stirring time is less than 10 minutes, problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if the stirring time exceeds 30 minutes, problems may occur in energy efficiency. In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, if the stirring speed is less than 2,000 rpm, problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if the stirring speed exceeds 4,000 rpm, problems may occur in energy efficiency. The step of forming nanovesicles by including betulin within the micelle structure can be carried out at room temperature and stirred using a homodisper, but is not limited thereto and is characterized by being able to be carried out under various temperature conditions and with various stirring methods. However, in the method for manufacturing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step of forming nanovesicles by including betulin within the micelle structure is too low, problems may occur in the formation of nanovesicles and problems may occur in the reprecipitation of betulin, and if the temperature is too high, water may evaporate, which may cause problems in the stability of nanovesicles.In addition, in the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the stirring speed is too low and the stirring time is too short during the step in which the betulin is included inside the micelle structure to form nanovesicles, the size of the nanovesicles may become non-uniform, problems with stability may occur, and problems with reprecipitation of betulin may occur; conversely, if the stirring speed is too high and the stirring time is too long, problems with process efficiency and stability may occur.

[0103] FIG. 4 is another flowchart illustrating a method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention.

[0104] According to FIG. 4, the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention comprises the steps of: adding 0.1 to 20 parts by weight of arginine to 58 to 99.8 parts by weight of water and stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; adding 0.002 to 2 parts by weight of quercetin and 0.098 to 20 parts by weight of caprylic acid to 50℃ to 90℃ while stirring with a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a secondb solution in which quercetin is dissolved in caprylic acid; and The method may be configured to include the step of adding the above-mentioned 2b solution to the first solution, heating it to 50°C - 90°C, stirring it at 2,000 rpm - 4,000 rpm for 10 - 30 minutes using a homodisper to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and the step of forming nanovesicles by including the quercetin inside the micelle structure.

[0105] In the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, the temperature of the step in which a first solution in which arginine is dissolved in water is generated can be carried out at room temperature, but is not limited thereto and has the characteristic that it can be carried out under various temperature conditions. However, in the method for manufacturing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step in which the first solution in which arginine is dissolved in water is generated is too low, then in the subsequent step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, due to the low temperature of the first solution, it takes too much time to heat the first solution to 50°C - 90°C when the secondb solution is added, and a problem may occur in which quercetin dissolved in the secondb solution reprecipitates. Furthermore, if the temperature of the step in which the first solution in which arginine is dissolved in water is generated is too high, then in the subsequent step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine In the step where micelles are formed, water may evaporate due to the high temperature of the first solution, and a problem may occur in which arginine reprecipitates from the first solution. In the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step in which the second solution (2b) in which quercetin is dissolved in caprylic acid is produced is less than 50°C, a problem may occur in which quercetin is not sufficiently dissolved in caprylic acid, and if it exceeds 90°C, a problem may occur in which the off-flavor of caprylic acid becomes severe, making it unsuitable for application in a cosmetic composition.In the step where the second solution of 2b is produced, a homodisper may be used for stirring, but it is not limited to this, and various stirring methods may be utilized. If the stirring time is less than 15 minutes in the step where the second solution of quercetin dissolved in caprylic acid is produced, a problem may occur where quercetin is not sufficiently dissolved in caprylic acid and residue remains, and if the stirring time exceeds 25 minutes, a problem may occur with energy efficiency. If the stirring speed is less than 1,500 rpm in the step where the second solution of quercetin dissolved in caprylic acid is produced, a problem may occur where quercetin is not sufficiently dissolved in caprylic acid and residue remains, and if the stirring speed exceeds 2,500 rpm, a problem may occur with energy efficiency. Furthermore, if the temperature of the step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine is less than 50°C, problems may arise in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine; if the temperature exceeds 90°C, the off-flavor of the caprylic acid may become severe, making it unsuitable for application in cosmetic compositions, and water may evaporate, which may cause problems with the stability of the micelles. A homodisper may be used for stirring during the step in which micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, but is not limited thereto.In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, if the stirring time is less than 10 minutes, problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if the stirring time exceeds 30 minutes, problems may occur in energy efficiency. In the step where micelles are formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, if the stirring speed is less than 2,000 rpm, problems may occur in the formation of ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and if the stirring speed exceeds 4,000 rpm, problems may occur in energy efficiency. The step of forming nanovesicles by including quercetin within the micelle structure can be carried out at room temperature and stirred using a homodisper, but is not limited thereto and is characterized by being able to be carried out under various temperature conditions and with various stirring methods. However, in the method for manufacturing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the temperature of the step of forming nanovesicles by including quercetin within the micelle structure is too low, problems may occur in the formation of nanovesicles and problems may occur in the reprecipitation of quercetin, and if the temperature is too high, water may evaporate, which may cause problems in the stability of the nanovesicles.In addition, in the method for preparing a cosmetic composition for improving photoaging and thermal aging according to the present invention, if the stirring speed is too slow and the stirring time is too short during the step in which the quercetin is included inside the micelle structure to form nanovesicles, the size of the nanovesicles may become non-uniform and problems with stability may occur, and problems with reprecipitation of quercetin may occur; and if the stirring speed is too fast and the stirring time is too long, problems with process efficiency and stability may occur.

[0106] [Example 1, Example 2]

[0107] Composition Example 1 (wt%) Example 2 (wt%) Water 64.00 64.00 Arginine 18.00 18.00 Caprylic acid 18.00 17.64 Betulin -0.36 Total 100.00 100.00

[0108] According to the method for preparing a cosmetic composition for improving photoaging and thermal aging of Fig. 2, 18.00 parts by weight of arginine were added to 64.00 parts by weight of water and stirred at 2,000 rpm for 20 minutes using a homodisper to produce a first solution in which arginine is dissolved in water. Then, 18.00 parts by weight of caprylic acid were added to the first solution while heating to 70°C and stirring at 3,000 rpm for 20 minutes using a homodisper to prepare the cosmetic composition for improving photoaging and thermal aging of Example 1, which includes micelles formed by ion pairs resulting from hydrogen bonding between the caprylic acid and the arginine. According to the method for preparing a cosmetic composition for improving photoaging and thermal aging of Fig. 3, arginine 18.00 parts by weight are added to 64.00 parts by weight of water and stirred at 2,000 rpm for 20 minutes using a homodisper to produce a first solution in which arginine is dissolved in water, and 0.36 parts by weight of betulin and 17 parts of caprylic acid.A cosmetic composition for improving photoaging and thermal aging according to Example 2 was prepared by heating 64 parts by weight to 70°C and stirring at 2,000 rpm for 20 minutes using a homodisper to produce a second solution in which betulin is dissolved in caprylic acid, adding the second solution to the first solution while heating to 70°C and stirring at 3,000 rpm for 20 minutes using a homodisper to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine, and including a nanovesicle formed by containing betulin inside the micelle structure.

[0109] In both Example 1 and Example 2, it was confirmed that an ion pair formed by two strong hydrogen bonds between the guanidyl group of arginine and the carboxyl group of caprylic acid, and in Example 1, a spherical particle called a micelle was formed by self-assembly, and in Example 2, a nanovesicle was formed by additionally including betulin inside the micelle structure. In Examples 1 and 2, it was confirmed that the compatibility between arginine and caprylic acid, which has poor compatibility, can be significantly improved due to the formation of ion pairs by two strong hydrogen bonds between the guanidyl group of arginine and the carboxyl group of caprylic acid, and that it is possible to prepare a cosmetic composition containing both arginine and caprylic acid without a separate surfactant and additional oil composition. In addition, in Example 2, it was confirmed that the compatibility between arginine and betulin, which has poor compatibility, can be improved by forming a nano vesicle that carries betulin and has an amine group or a carboxyl group of arginine as a hydrophilic head, and that the poorly soluble betulin can be sufficiently applied to water-based cosmetic formulations (skin toner, ampoule, essence, etc.) that provide aesthetic pleasure through the formation of a nano vesicle that carries betulin and has an amine group or a carboxyl group as a hydrophilic head.Furthermore, in Example 2, it was confirmed that the anti-inflammatory and anti-aging effects exhibited by the nano vesicle carrying betulin and having the amine group or carboxyl group of arginine as a hydrophilic head were significantly increased compared to the anti-inflammatory and anti-aging effects exhibited by the arginine and betulin individually. Due to this complex synergistic effect, it is possible to manufacture cosmetics suitable for skin sensitive to photoaging and thermal aging, and it is possible to apply them to various formulations including skin toner formulations, ampoule formulations, essence formulations, lotion formulations, cream formulations, mask formulations, and pad formulations, thereby maximizing aesthetic pleasure and efficacy for various parts of the human body and skin conditions.

[0110] [Experimental Example 1] pH measurement of micelles and nanovesicles

[0111] Figure 5 is a diagram showing the change in pH as the content of caprylic acid is increased from 1% to 12% while the content of arginine is fixed (10%).

[0112] In the left diagram of Fig. 5, the pH change according to the increase in caprylic acid content is shown when caprylic acid and arginine are simply mixed without forming hydrogen bonds between them. It was confirmed that as the caprylic acid content increased, the pH decreased from 9.7 to 6.0, indicating a change from basic to acidic. In contrast, in the middle diagram of Fig. 5, the pH change according to the increase in caprylic acid content is shown when micelles are formed by ion pairs formed by hydrogen bonds between caprylic acid and arginine. It was confirmed that even as the caprylic acid content increased, the pH remained around 7.0, indicating neutrality. In addition, the right-hand diagram in Fig. 5 shows the change in pH according to the increase in caprylic acid content when nanovesicles are formed by containing betulin inside the micelle structure. It was confirmed that the pH remained around 7.0 and remained neutral despite the increase in caprylic acid content. Through this change in pH shown in Fig. 5, the existence of micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine, and the existence of nanovesicles formed by containing betulin inside the micelle structure can be confirmed. Furthermore, since a change in pH occurs when betulin exceeds 0.5%, it was confirmed that betulin can be loaded inside the micelle structure up to a maximum of 0.5%.

[0113] [Experimental Example 2] Measurement of the Solubility of Micelles and Nano Vesicles in Water

[0114] Micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine can have the amine or carboxyl group of arginine forming the hydrophilic head and the 8-carbon chain of caprylic acid forming the hydrophobic tail. Through this process, the water solubility of caprylic acid and arginine can be increased. Caprylic acid is insoluble in water, and when arginine is dissolved in water alone, arginine exhibits a positive charge, allowing it to form partial intermolecular bonds with oxygen ions in water and achieve a solubility of approximately 14.87 g / 100 mL (at 20°C). It was found that when an ion pair is formed between caprylic acid and arginine through strong hydrogen bonding between opposite charges, the solubility of both caprylic acid and arginine in water increases. Although caprylic acid is insoluble in water, it exhibits a distinct hydrophilic head structure and a hydrophobic tail structure due to the ion pair formed with arginine, thereby becoming soluble in water. According to the cosmetic composition for improving photoaging and thermal aging containing micelles formed by the ion pair formed by hydrogen bonding between the caprylic acid and arginine in Example 1, it was confirmed that the solubility of caprylic acid and arginine in water increases.Although caprylic acid was found to be insoluble in water, it was confirmed that the solubility of caprylic acid in water increased to 25 g / 100 mL (20°C) by forming an ion pair with arginine. In addition, although arginine alone was found to have a solubility of 14.87 g / 100 mL (20°C), it was confirmed that the solubility of arginine in water increased by more than 68% to 25 g / 100 mL (20°C) by forming an ion pair with caprylic acid.

[0115] [Experimental Example 3] Comparison of the characteristics of micelles and nanovesicles

[0116] The appearance of the micelle formed by the ion pair formed by hydrogen bonding between caprylic acid and arginine in Example 1 and the nanovesicle formed by containing betulin inside the micelle structure in Example 2 were photographed and compared.

[0117] Figure 6 is a photograph comparing the characteristics of a micelle formed by an ion pair formed by hydrogen bonding between caprylic acid and arginine in Example 1 and a nanovesicle formed by containing betulin inside the micelle structure in Example 2.

[0118] According to Fig. 6, micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine exhibit a transparent appearance as light is transmitted, but nanovesicles formed by containing betulin inside the micelle structure exhibit a suspension appearance as light is scattered and the size increases due to the large amount of betulin inside the micelle structure. In the case of a suspension containing a nanovesicle formed by including betulin inside the micelle structure of Example 2, if it is not stabilized using a surfactant or the like, particle aggregation may occur, phase separation may occur, or precipitates may form inside. However, it was confirmed that the nanovesicle formed by including betulin inside the micelle structure of Example 2 maintained stability for more than 3 months at both high temperature (50°C) and low temperature (-20°C) without such problems. Furthermore, as the nanovesicle formed by including betulin inside the micelle structure of Example 2 maintained stability for more than 3 months, it was confirmed that betulin, which is insoluble in water, was supported inside the micelle structure, and its solubility in water increased to 0.5 g / 100 mL.

[0119] [Experimental Example 4] Electron microscopy (TEM) imaging of micelles and nanovesicles

[0120] Electron microscope (TEM) images were taken to compare the micelle formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1 and the nanovesicle formed by containing betulin inside the micelle structure in Example 2.

[0121] Figure 7 is a comparison of electron microscope (TEM) images of a micelle formed by an ion pair formed by hydrogen bonding between caprylic acid and arginine in Example 1 and a nanovesicle formed by containing betulin inside the micelle structure in Example 2.

[0122] According to Figure 7, it was confirmed that the diameter of the nanovesicle formed by containing betulin inside the micelle structure is larger than the diameter of the micelle formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine. In addition, it was confirmed that the nanovesicle formed by containing betulin inside the micelle structure has a double membrane structure with betulin loaded inside.

[0123] [Experimental Example 5] Measurement of diameter and polydispersity index of micelles and nanovesicles using dynamic light scattering (DLS), and measurement of surface charge via zeta potential

[0124] In Table 2, the diameter and polydispersity index were measured and compared using dynamic light scattering (DLS) for micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1 and nanovesicles formed by containing betulin inside the micelle structure in Example 2, and the surface charge was measured and compared through zeta potential.

[0125] For the cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and the cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2, the diameter and polydispersity index were measured using dynamic light scattering (DLS), and the surface charge was measured via zeta potential. Dynamic light scattering (DLS) and zeta potential measurements were performed using the ELSZ-Neo model from Otsuka Electronics, and the same sample was measured three times at 25°C. After sample preparation, the sample was placed in a column for zeta potential measurement, and measurements were started within 120 seconds to obtain the results. As a result, according to Table 2, it was confirmed that Example 1 contained micelles with an average size of 4.9±0.2 nm and exhibited a surface charge of -9.98 mV. Meanwhile, it was confirmed that Example 2 contained nano vesicles with an average size of 471.9±10.3 nm and exhibited a surface charge of -9.91 mV. These results were attributed to the structural characteristics of the nano vesicles, in which betulin was stably loaded inside the micelles, causing a structural change from micelles to nano vesicles and a growth in diameter of more than 96 times.In addition, since there is no externally exposed betulin in the case of nano vesicles, it was confirmed through zeta potential measurement results that nano vesicles and micelles have the same external surface and that nano vesicles have a surface charge equivalent to that of micelles.

[0126] Sample Diameter (nm) Polydispersity Index Zeta Potential (mV) Example 1 4.9±0.2 nm 0.007-9.98 Example 2 4.7 1.9±10.3 0.327-9.91

[0127] [Experimental Example 6] 1H-NMR Analysis of Micelles and Nanovesicles

[0128] 1H-NMR was measured and analyzed for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0129] Figure 8 is a diagram comparing the results of measuring 1H-NMR for caprylic acid, arginine, and betulin with the results of measuring 1H-NMR for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0130] According to Fig. 8, the following changes may occur due to hydrogen bonding between caprylic acid and arginine. Peak A of caprylic acid is extinguished due to hydrogen bonding with arginine. Peak B of caprylic acid shifts △0.515 to a higher field due to hydrogen bonding with arginine (B peak 2.508→1.993 dramatically up shift), and Peak C of caprylic acid shifts △0.039 to a higher field due to hydrogen bonding with arginine (C peak 1.507→1.468 up shift). The D peak of caprylic acid shifts △0.300 to a higher field due to hydrogen bonding with arginine (D peak 1.300→1.000 up shift), and the E peak of caprylic acid shifts △0.177 to a higher field due to hydrogen bonding with arginine (D peak 0.864→0.687 up shift). The b peak (near amine) and c peak (near guanidyl) of arginine move further apart from each other due to hydrogen bonding with caprylic acid (b peak 3.277→3.584 dramatically down shift, c peak 3.228→3.041 dramatically up shift). In addition, the d peak of arginine splits into two peaks due to hydrogen bonding with caprylic acid (d peak 1.645→1.746 / 1.538 split).According to Fig. 8, due to hydrogen bonding between the guanidyl group of arginine and the carboxyl group of caprylic acid, electrons are supplied to the guanidyl group (transfer of non-covalent electron pairs of the carboxyl group through intermolecular bonding as cations), and as a result, electrons are indirectly supplied to the c and d protons of arginine, which can cause a change in peaks. In particular, in 1H-NMR, two structurally equivalent d protons may split into two peaks because the degree of electron influence differs depending on the difference in distance from the guanidyl group. Due to the electron pair supplied to the guanidyl group through hydrogen bonding with caprylic acid, electrons are indirectly supplied to the c proton of arginine and can shift to a higher field of △0.187. The A proton of caprylic acid dissociates due to hydrogen bonding with arginine, and the peak disappears. Among the B, C, D, and E protons of caprylic acid, the B proton, which is close to the carboxyl group, can be significantly affected by hydrogen bonding between the guanidyl group of arginine and the carboxyl group of caprylic acid; consequently, the B peak of caprylic acid can shift the most significantly to a high point due to hydrogen bonding with arginine.On the other hand, in the case of Example 2, since no change occurred in the betulin peak, it was confirmed that the entire betulin was loaded inside the micelle formed by the ion pair between arginine and caprylic acid without any intermolecular bonding with other external molecules.

[0131] [Experimental Example 7] FT-IR Analysis of Micelles and Nanovesicles

[0132] FT-IR was measured and analyzed for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0133] Figure 9 is a diagram comparing the results of FT-IR measurements for caprylic acid, arginine, and betulin with the results of FT-IR measurements for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0134] FT-IR measurements were performed at 1 cm per point using the Vertex 70 and Hyperion 2000 (FTIR spectrophotometer, Bruker Instruments). -14000~500cm at the data collection speed -1 The spectral range was measured and analyzed. Through FT-IR analysis, changes due to hydrogen bonding between the guanidyl group of arginine and the carboxyl group of caprylic acid were confirmed. Meanwhile, through FT-IR measurements of Example 2 and betulin, it was confirmed that betulin was stably loaded inside the nano vesicle, and that the functional group of betulin was not present on the nano vesicle surface functional group measured by infrared (IR).

[0135] According to Fig. 9, the following changes may occur due to hydrogen bonding between caprylic acid and arginine. Due to the ion pair formed by hydrogen bonding with arginine, the peak of the carbonyl group of caprylic acid (1705 cm⁻¹) -1 Changes may occur in ), and changes may also occur in the hydroxyl group peak (check the blue shading in Fig. 9). Due to the ion pair formed by hydrogen bonding with caprylic acid, the amine group peak of arginine (1643 cm⁻¹) -1 A red shift may occur, and an increase in wavelength and a decrease in frequency may occur. On the other hand, in the case of Example 2, the peak of the betulin functional group disappeared. This is judged to be a phenomenon that occurs because the betulin is entirely loaded inside the micelle formed by the ion pair between arginine and caprylic acid, making it impossible for the betulin functional group to absorb infrared (IR).

[0136] [Experimental Example 8] Differential Scanning Calorimetry (DSC) Analysis of Micelles and Nanovesicles

[0137] Differential Scanning Calorimetry (DSC) was measured and analyzed for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin contained within the micelle structure in Example 2.

[0138] Figure 10 is a diagram comparing the results of Differential Scanning Calorimetry (DSC) measurements for betulin with the results of Differential Scanning Calorimetry (DSC) measurements for a cosmetic composition for improving photoaging and thermal aging containing micelles formed by hydrogen bonding between caprylic acid and arginine in Example 1, and a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin being included inside the micelle structure in Example 2.

[0139] Thermal properties of betulin and Examples 1 and 2 were measured using a Differential Scanning Calorimetry (DSC, Mettler-Toledo) instrument. The accuracy of heat flow and temperature was 0.0001 mW and 0.01 K, respectively. Measurements were taken at 20 cc min -1 flow rate and 10K min -1It was performed in a nitrogen atmosphere at a heating rate. After measurement, the results were graphed using analysis software (STARe, Mettler-Toledo).

[0140] According to Fig. 10, the analysis results of betulin and Examples 1 and 2, confirmed by thermal analysis using Differential Scanning Calorimetry (DSC, Mettler-Toledo), are as follows. In the case of Example 1, it was confirmed that the thermal properties individually exhibited by caprylic acid and arginine were eliminated due to the micelle structure formed by ion pairs resulting from hydrogen bonding between caprylic acid and arginine. Additionally, according to Fig. 10, when betulin was heated alone and examined in the 250-270°C range, a phase transition with a peak at 257°C was observed; however, in the case of Example 2, it was confirmed that the corresponding transition was not observed because betulin was supported internally.

[0141] [Experimental Example 9] Antioxidant activity experiment on a cosmetic composition for improving photoaging and thermal aging containing arginine and betulin, and nanovesicles formed by including betulin inside the micelle structure of Example 2

[0142] Antioxidant activity was confirmed through DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis for a cosmetic composition for improving photoaging and thermal aging containing arginine and betulin, and nanovesicles formed by including betulin inside the micelle structure of Example 2.

[0143] FIG. 11 is a diagram showing the antioxidant activity evaluated through DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis for a cosmetic composition for improving photoaging and thermal aging, which includes arginine, betulin, and nanovesicles formed by including betulin inside the micelle structure of Example 2.

[0144] DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis is a test method that measures a sample's ability to scavenge free radicals. It verifies the scavenging ability of free radicals, which is determined by the sample's reducing power, through absorbance. DPPH (2,2-diphenyl-1-picrylhydrazyl) is a stable free radical and is a purple mixture; when antioxidants donate electrons to reduce the free radicals, the color of DPPH (2,2-diphenyl-1-picrylhydrazyl) may change. In other words, DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis confirms the free radical scavenging ability of a sample by measuring the color change of DPPH (2,2-diphenyl-1-picrylhydrazyl).

[0145] A mixture was prepared by dissolving 100 μM DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals in 100% ethanol, vigorously shaking the mixture, and leaving it in the dark at 23°C for 30 minutes. The resulting dark blue DPPH (2,2-diphenyl-1-picrylhydrazyl) radical solution was adjusted to an absorbance of 0.5 ± 0.05 at 517 nm. Test substances (10 μL each) were added to 190 μL of the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical solution present in a 96-well microplate. The control group consisted of 10 μL of 99% ethanol and 190 μL of the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical solution. The decrease in absorbance resulting from the addition of the test substances was measured at 734 nm using an ELISA reader. The DPPH(2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity of the test substances was expressed as a percentage of the remaining DPPH(2,2-diphenyl-1-picrylhydrazyl) radicals at each time point. All test substances were measured and analyzed in three repetitions.

[0146] According to Figure 11, the antioxidant activity of arginine and betulin is excellent in the beginning, but as the concentration increases, there is a limit to the increase in the antioxidant activity with increasing concentration. In contrast, in the case of Example 2, the increase in antioxidant activity with increasing concentration was found to be superior to that of arginine and betulin, and it was confirmed that the antioxidant activity increased proportionally with increasing concentration. These results indicate that when a cosmetic composition for improving photoaging and thermal aging containing arginine and betulin at high concentrations, and nanovesicles formed by including betulin inside the micelle structure of Example 2, is used, the cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by including betulin inside the micelle structure of Example 2 has superior antioxidant activity.

[0147] [Experimental Example 10] Evaluation of Inhibition of Inflammation Induction by Ultraviolet (Ultraviolet B, UVB)

[0148] Human-derived keratinocytes are cultured in a 6-well plate in a 5% CO2 incubator using DMEM medium (Addexbio). Arginine, betulin, and Example 2, which have antioxidant and anti-inflammatory effects, are treated for 30 minutes, and UVB ultraviolet rays are irradiated at 25 mW / cm². 2Inflammation was induced by treating with an intensity of 15 minutes. Consequently, inflammatory cytokines that directly affect cell death, skin wrinkle formation, and deterioration of elasticity were induced by ultraviolet light, and the concentrations of arginine, betulin, and cytokines inhibited by Example 2 were analyzed. The concentrations were quantified using an Enzyme-Linked Immunosorbent Assay (ELISA), and the results are shown in Figures 12, 13, and 14. The experiment was evaluated by repeating it three times, and the results confirmed that all items were significant within a confidence interval of at least 95% (with a sample error of ±5%).

[0149] The types of inflammatory cytokines evaluated in this experiment and their roles in the skin's inflammatory response are as follows.

[0150] MCP-1: A chemokine involved in the recruitment of macrophages and monocytes, influencing various processes of the inflammatory response. It is an inflammatory response initiator secreted by skin cells.

[0151] IL4: A cytokine that regulates immune and inflammatory responses, playing a role in converting B lymphocyte products into IgE. As an inflammatory factor that causes skin barrier deterioration, it leads to chronic itching, an increased risk of external infections, and chronic inflammation. It is a key factor in the development of skin inflammation as a disease.

[0152] IL8: An inflammatory cytokine that amplifies inflammatory responses. It plays a role in activating and recruiting neutrophils, and its expression is promoted by UV stimulation, leading to increased inflammation. It is an inflammatory response mediator primarily secreted by macrophages and epithelial cells.

[0153] In FIGS. 12, 13, and 14, the anti-inflammatory efficacy of arginine, betulin, and Example 2 against three inflammatory cytokines was confirmed. According to FIGS. 12, 13, and 14, the anti-inflammatory efficacy against inflammatory cytokines was individually confirmed for the antioxidant components arginine and betulin, but Example 2, which contains both arginine and betulin, was found to exhibit superior anti-inflammatory efficacy compared to the individual anti-inflammatory efficacy of arginine or betulin against inflammatory cytokines. That is, in Example 2, which contains both arginine and betulin, a significant increase in anti-inflammatory efficacy due to the synergy of arginine and betulin was confirmed.

[0154] According to Figure 12, in the case of the cytokine MCP-1, which initiates an inflammatory response, an anti-inflammatory efficacy of up to 35.2% (100 ppm) was confirmed when treated with arginine alone and up to 35.8% (100 ppm) when treated with betulin alone, and an anti-inflammatory efficacy of up to 67.6% (100 ppm) was confirmed when Example 2 was applied. Consequently, in Example 2, an increase in anti-inflammatory efficacy of 92% compared to arginine and 88% compared to betulin was confirmed.

[0155] According to Figure 13, in the case of IL4, which causes diseases due to deterioration of the skin barrier, an anti-inflammatory efficacy of up to 68.5% (100 ppm) was confirmed when treated with arginine alone and up to 72.5% (100 ppm) when treated with betulin alone, and an anti-inflammatory efficacy of up to 98.0% (100 ppm) was confirmed when Example 2 was applied. Consequently, in Example 2, an increase in anti-inflammatory efficacy of 43.1% compared to arginine and 35.2% compared to betulin was confirmed.

[0156] According to Figure 14, the cytokine IL8, which mediates inflammatory responses, showed an anti-inflammatory efficacy of up to 51.8% (100 ppm) when treated with arginine alone and up to 66.0% (100 ppm) when treated with betulin alone, and up to 90.9% (100 ppm) when Example 2 was applied. Consequently, in Example 2, an increase in anti-inflammatory efficacy of 75.4% compared to arginine and 37.7% compared to betulin was confirmed.

[0157] [Experimental Example 11] Comparison of a lotion formulation using a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin inside micelle structures formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine, and a lotion formulation using a cosmetic composition for improving photoaging and thermal aging containing nanovesicles formed by betulin inside micelle structures formed by ion pairs formed by hydrogen bonding between caprylic / capric triglyceride and arginine

[0158] Composition Ratio (%) Cosmetic composition for improving photoaging and thermal aging comprising nanovesicles formed by containing betulin inside a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine 3.00 Purified water 67.12 Carbomer 0.10 PEG / PPG / Polybutylene Glycol-8 / 5 / 3 Glycerin 1.50 Trisodium EDTA 0.04 Urea 1.85 Hydroxyethylpiperazine Ethanesulfonic Acid 1.85 Trehalose 2.50 Panthenol 1.35 2,3-Butanediol 5.00 1,2-Hexanediol 1.50 PEG / PPG-17 / 6 Copolymer 1.0 0 Glycereth-26 1.22 Tromethamine 0.10 Polyacrylate Crosspolymer-60 14 Caprylic Methicone 1.23 Squalane 2.50 Caprylic / Capric Triglyceride 1.50 Dimethicone 2.20 PCA Dimethicone 2.50 Dimethiconol 0.60 Silica 0.10 Sorbitan Oliveate 0.30 Cetyl Malmitate 0.30 Glyceryl Stearate 0.50

[0159] Composition Ratio (%) Cosmetic composition for improving photoaging and thermal aging comprising nanovesicles formed by betulin contained within a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic / capric triglycerides and arginine 3.00 Purified water 67.12 Carbomer 0.10 PEG / PPG / Polybutylene Glycol-8 / 5 / 3 Glycerin 1.50 Trisodium EDTA 0.04 Urea 1.85 Hydroxyethylpiperazine Ethanesulfonic Acid 1.85 Trehalose 2.50 Panthenol 1.35 2,3-Butanediol 5.00 1,2-Hexanediol 1.50 PEG / PPG-17 / 6 Copolymer 1.0 0 Glycereth-26 1.22 Tromethamine 0.10 Polyacrylate Crosspolymer-60 14 Caprylic Methicone 1.23 Squalane 2.50 Caprylic / Capric Triglyceride 1.50 Dimethicone 2.20 PCA Dimethicone 2.50 Dimethiconol 0.60 Silica 0.10 Sorbitan Oliveate 0.30 Cetyl Malmitate 0.30 Glyceryl Stearate 0.50

[0160] A lotion formulation was prepared using a cosmetic composition for improving photoaging and thermal aging, comprising nanovesicles formed by betulin contained within a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine according to Table 3; and a lotion formulation was prepared using a cosmetic composition for improving photoaging and thermal aging, comprising nanovesicles formed by betulin contained within a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic / capric triglyceride and arginine according to Table 4, and then aesthetic differences were compared. The above lotion formulations [contain] ion pairs formed by hydrogen bonding between caprylic acid and arginine A cosmetic composition for improving photoaging and thermal aging can be prepared by including 3% of a nanovesicle formed by containing betulin inside a micelle structure formed by ion pairs), and a cosmetic composition for improving photoaging and thermal aging can be prepared by including 3% of a nanovesicle formed by containing betulin inside a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic / capric triglyceride and arginine. The water-soluble components and polymer components (14 components of purified water - polyacrylate crosspolymer-6) of Tables 3 and 4 are stirred at 2000 rpm at 40°C for 20 minutes.Meanwhile, the oil-soluble components of Tables 3 and 4 (10 components of caprylic methicone - glyceryl stearate) are stirred separately at 2000 rpm for 20 minutes at 40°C, and then the stirred oil-soluble components of Tables 3 and 4 (10 components of caprylic methicone - glyceryl stearate) are added to the stirred water-soluble components and polymer components of Tables 3 and 4 (14 components of purified water - polyacrylate crosspolymer-6), and emulsified using a homogenizer at 5000 rpm for 20 minutes at 40°C. Afterward, a lotion formulation may be prepared by adding a cosmetic composition for improving photoaging and thermal aging that contains a nanovesicle formed by betulin inside a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine, and a cosmetic composition for improving photoaging and thermal aging that contains a nanovesicle formed by betulin inside a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic / capric triglyceride and arginine, and the method of preparing the lotion formulation is not limited to the described method. The lotion formulations shown in Tables 3 and 4 above can aesthetically improve skin texture and provide a sense of softness, and can also provide a sense of soothing relief from skin irritation caused by light and heat after showering and outdoor activities.

[0161] Table 5 shows the aesthetic difference between a lotion formulation using a cosmetic composition for improving photoaging and thermal aging that contains a nanovesicle formed by betulin inside a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine according to Table 3, and a lotion formulation using a cosmetic composition for improving photoaging and thermal aging that contains a nanovesicle formed by betulin inside a micelle structure formed by ion pairs formed by hydrogen bonding between caprylic / capric triglyceride and arginine according to Table 4. According to Table 5, a lotion formulation using a cosmetic composition for improving photoaging and thermal aging, containing nanovesicles formed by betulin inside micelle structures formed by ion pairs formed by hydrogen bonds between caprylic acid and arginine, showed excellent soothing effects, and a lotion formulation using a cosmetic composition for improving photoaging and thermal aging, containing nanovesicles formed by betulin inside micelle structures formed by ion pairs formed by hydrogen bonds between caprylic / capric triglyceride and arginine, showed excellent effects in terms of moisturizing sensation and fragrance.

[0162] Lotion formulation in Table 3 Lotion formulation in Table 4 Skin softening after application 4 points 5 points Moisture felt after application 4 points 5 points Skin soothing feeling when applied after showering 5 points 4 points Skin soothing feeling when applied after outdoor activities 5 points 4 points Satisfactory scent on the skin after application 4 points 5 points

[0163] (5 points: Very satisfied, feeling significant improvement compared to before application; 4 points: Relatively satisfied, feeling improvement compared to before application; 3 points: No difference felt compared to before application; 2 points: Dissatisfied, feeling worsening compared to before application; 1 point: Feeling significant skin deterioration compared to before application, accompanied by irritation)

[0164] [Experimental Example 12] Evaluation of Antibacterial Efficacy

[0165] To evaluate the antibacterial efficacy of a cosmetic composition for improving photoaging and thermal aging containing micelles formed by hydrogen bonding between caprylic acid and arginine according to the present invention, 9 mL of a diluent was added to 1 g of the cosmetic composition for improving photoaging and thermal aging to prepare a 10-fold diluted solution. Then, 1 mL was spread onto at least two agar plates according to the agar plate plating method on an agar medium according to the total viable cell count test method. The antibacterial efficacy was evaluated by incubating bacteria at 30-35°C for 48 hours and fungi at 20-25°C for 5 days. The results of this evaluation were compared with the results of an arginine aqueous solution.

[0166] Aqueous solution of arginine with antibacterial and antifungal inhibitory properties -- Aqueous solution of a cosmetic composition for improving photoaging and thermal aging containing micelles formed by ion pairs formed by hydrogen bonding between caprylic acid and arginine according to the present invention++

[0167] (- : detection of bacteria or fungi, + : non-detection of bacteria or fungi) According to Table 6, when the above arginine aqueous solution was treated with bacteria and fungi, both bacteria and fungi were detected, indicating that the above arginine aqueous solution does not have antibacterial efficacy. Conversely, according to Table 6, when the above cosmetic composition aqueous solution for improving photoaging and thermal aging was treated with bacteria and fungi, neither bacteria nor fungi were detected, indicating that the above cosmetic composition aqueous solution for improving photoaging and thermal aging has antibacterial efficacy.

[0168] As described above, the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for preparing the same include one or more micelles formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or micelles formed by hydrogen bonding between arginine and one or more triglycerides containing 8 to 12 carbon atoms, thereby enhancing the penetration of saturated fatty acids and arginine through the keratin and their solubility in water. Furthermore, in the cosmetic composition for improving photoaging and thermal aging according to the present invention and the method for preparing the same, a nanovesicle can be formed by additionally including a poorly soluble component such as betulin or quercetin in one or more micelles formed by ion pairs formed by hydrogen bonding between arginine and one or more saturated fatty acids having 8 to 12 carbon atoms, or in one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms, and in one or more micelles formed by ion pairs formed by hydrogen bonding between arginine and one or more triglycerides containing one or more saturated fatty acids having 8 to 12 carbon atoms, thereby enhancing the penetration of the poorly soluble component such as betulin or quercetin through the keratin and its solubility in water.As a result, by means of a nanovesicle structure containing a sparingly soluble component such as betulin or quercetin, a triglyceride containing one or more of the above-mentioned arginine, a saturated fatty acid having 8 to 12 carbon atoms, or a saturated fatty acid having 8 to 12 carbon atoms, the side effects can be reduced compared to when arginine and sparingly soluble components such as betulin or quercetin are used alone, and the combined and enhanced effects of reducing inflammation caused by photoaging and thermal aging, alleviating symptoms of aging, and improving the skin can be achieved.

[0169] As described above, the present invention has been explained with reference to various embodiments and experimental examples, but this is merely illustrative, and it should be understood that various modifications and equivalent alternative embodiments are possible based on the ordinary knowledge in the field to which the technology belongs. Accordingly, the true technical scope of protection of the present invention is determined by the claims described below and should be determined based on the specific details of the invention described above.

[0170] The present invention relates to a cosmetic composition for improving photoaging and thermal aging and a method for manufacturing the same, and is applicable in industrial fields related to cosmetic manufacturing.

Claims

1. A cosmetic composition for improving photoaging and thermal aging, comprising one or more micelles formed by ion pairs formed by hydrogen bonding between arginine and one or more of saturated fatty acids having 8 to 12 carbon atoms, or micelles formed by ion pairs formed by hydrogen bonding between arginine and one or more of triglycerides containing 8 to 12 carbon atoms.

2. In Paragraph 1, A cosmetic composition for improving photoaging and thermal aging, characterized in that the micelles have an average diameter of 1-10 nm.

3. In Paragraph 1, A cosmetic composition for improving photoaging and thermal aging, characterized by the additional inclusion of a sparingly soluble component within the micelle structure to form a nanovesicle.

4. In Paragraph 3, A cosmetic composition for improving photoaging and thermal aging, characterized in that the above nanovesicles have an average diameter of 100-500 nm.

5. In Paragraph 3, A cosmetic composition for improving photoaging and thermal aging, characterized in that the above-mentioned insoluble component includes one or more of betulin or quercetin.

6. In paragraphs 1 through 5, A cosmetic composition for improving photoaging and thermal aging, characterized in that the above-mentioned saturated fatty acid having 8 to 12 carbon atoms has a carboxyl group only at the 1st carbon and has a linear structure rather than a branched structure.

7. In Paragraph 6, A cosmetic composition for improving photoaging and thermal aging, characterized in that the saturated fatty acid having a linear structure with 8 to 12 carbon atoms is caprylic acid, capric acid, or lauric acid.

8. In Paragraph 7, A cosmetic composition for improving photoaging and thermal aging, characterized by additionally including a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms, and not having a branched structure.

9. In Paragraph 8, A cosmetic composition for improving photoaging and thermal aging, characterized in that the linear diol is 1,2-hexanediol.

10. In Paragraph 7, It includes micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine. The above-mentioned insoluble component is betulin, and A cosmetic composition for improving photoaging and thermal aging, characterized by comprising 0.1 to 20 parts by weight of arginine, 0.098 to 20 parts by weight of caprylic acid, 0.002 to 0.5 parts by weight of betulin, and 59.5 to 99.8 parts by weight of water.

11. In Paragraph 7, It includes micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine. The above-mentioned insoluble component is quercetin, and A cosmetic composition for improving photoaging and thermal aging, characterized by comprising 0.1 to 20 parts by weight of arginine, 0.098 to 20 parts by weight of caprylic acid, 0.002 to 2 parts by weight of quercetin, and 58 to 99.8 parts by weight of water.

12. In Paragraph 7, It includes micelles formed by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine. A cosmetic composition for improving photoaging and thermal aging, characterized in that the caprylic acid and the arginine have a molar ratio of 1:3 to 3:

1.

13. In paragraphs 1 through 5, A cosmetic composition for improving photoaging and thermal aging, characterized in that the triglyceride containing one or more of the above-mentioned saturated fatty acids having 8 to 12 carbon atoms is glyceryl tridecanoate, glyceryl trioctanoate, or caprylic / capric triglyceride.

14. In Paragraph 13, A cosmetic composition for improving photoaging and thermal aging, characterized by additionally including a linear diol having 6 to 12 carbon atoms, having hydroxyl groups at the 1st and 2nd carbon atoms, and not having a branched structure.

15. In Paragraph 14, A cosmetic composition for improving photoaging and thermal aging, characterized in that the linear diol is 1,2-hexanediol.

16. A step in which 0.1 to 20 parts by weight of arginine are added to 59.5 to 99.8 parts by weight of water, and stirred using a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; and A step in which 0.098 to 20 parts by weight of caprylic acid are added to the first solution, heated to 50°C to 90°C, and stirred at 2,000 rpm to 4,000 rpm for 10 to 30 minutes using a homodisper to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine; A method for preparing a cosmetic composition for improving photoaging and thermal aging, comprising 17. A step in which 0.1 to 20 parts by weight of arginine are added to 59.5 to 99.8 parts by weight of water, and stirred using a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; A step in which 0.002 - 0.5 parts by weight of betulin and 0.098 - 20 parts by weight of caprylic acid are heated to 50℃ - 90℃ and stirred using a homodisper at 1,500 rpm - 2,500 rpm for 15 - 25 minutes to produce a solution 2a in which betulin is dissolved in caprylic acid; A step in which the 2a solution is added to the 1st solution and heated to 50°C - 90°C, and stirred at 2,000 rpm - 4,000 rpm for 10 - 30 minutes using a homodisper, thereby forming micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine; and A step in which the betulin is included inside the micelle structure to form a nanovesicle; A method for preparing a cosmetic composition for improving photoaging and thermal aging, comprising 18. A step in which 0.1 to 20 parts by weight of arginine are added to 58 to 99.8 parts by weight of water, and stirred using a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a first solution in which arginine is dissolved in water; A step in which 0.002 to 2 parts by weight of quercetin and 0.098 to 20 parts by weight of caprylic acid are heated to 50℃ to 90℃ and stirred using a homodisper at 1,500 rpm to 2,500 rpm for 15 to 25 minutes to produce a solution 2b in which quercetin is dissolved in caprylic acid; A step in which the 2b solution is added to the 1st solution, heated to 50°C - 90°C, and stirred at 2,000 rpm - 4,000 rpm for 10 - 30 minutes using a homodisper to form micelles by ion pairs formed by hydrogen bonding between the caprylic acid and the arginine; and A step in which the quercetin is included inside the micelle structure to form a nanovesicle; A method for preparing a cosmetic composition for improving photoaging and thermal aging, comprising

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