A real-time molecular BIO scanner for simulating and investigating intracellular reactions with artificial intelligence

Abstract

This invention is an advanced bioimaging device allowing for the observation and analysis of living cells and intracellular reactions in real-time without the need for staining. It uses a combination of nanophotonic microscopy, nanobiological sensors, artificial intelligence processing, and ultra-sensitive spectroscopy technology to record and analyze intracellular changes without destroying the biological structure. The key feature of this technology is non-invasive and live imaging of cells and tissues without the need to add dyes or external chemicals that can affect the normal behavior of the cell. It offers higher resolution, no chemical interference, and the ability to automatically analyze data compared to traditional imaging methods such as fluorescence and phase contrast. It also includes cell research, drug development, personalized medicine, biotechnology, nanotechnology, and medical diagnostics. This invention is designed to study molecular, cellular, and biological processes in living cells and can simultaneously perform imaging and data analysis.

Description

A REAL-TIME MOLECULAR BIO SCANNER FOR SIMULATING AND INVESTIGATING INTRACELLULAR REACTIONS WITH ARTIFICIAL INTELLIGENCE

[0001] Studying intracellular reactions and molecular processes is one of the boundless challenges in biology and medicine. Currently available devices require chemical dyes and chemical changes in cells to observe and analyze these processes, which can harm the normal functioning of cells. This invention uses nanosensors, nanomicroscopes and artificial intelligence processing to analyze molecular reactions inside cells without needing dyes or chemical changes. This device instantly monitors cellular reactions and allows for the observation of molecular processes in real time.

[0002] This invention specifically addresses the problems inherent in imaging and simulation methods of biological processes that usually require dyes. The main goal of the invention is to develop a device that allows researchers to more accurately observe intracellular processes and obtain molecular data in real time without damaging cells or altering their natural structure.

[0003] A61 - G16H - G01N 33 / 50 - G06F 19 / 00 - G05D 101 / 10 - C12N 15 / 00

[0004] US20210172944

[0005] Apparatus for analyzing and detecting interactions and reactions of molecules

[0006] An apparatus for label-free analysis of molecules, including interactions and reactions of the molecules, is disclosed. The apparatus is based on detecting molecule movement under the influence of an external electric field. The apparatus is able to achieve sensitive detection of molecular binding to proteins or other molecules, and conformational changes of proteins or other molecules and biochemical reactions of the proteins or other molecules. Applications of the apparatus include screening of drug molecules, kinetic analysis of posttranslational modification of proteins, and small molecule-protein interactions.

[0007] The device mentioned is based on detecting the movement of molecules under the influence of an external electric field using electrodes on a controlled surface in a solution. On the other hand, the claimed invention with using new nanoscale technologies, advanced nanoscale microscopes, and real-time processing by artificial intelligence, allows for live imaging of cells, recording, and analyzing cellular reactions.

[0008] US20020127591

[0009] Methods and systems for monitoring intracellular binding reactions

[0010] Intracellular binding reactions, and particularly DNA / DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby the second component is internalized within the biological cell. At least one of the first and second components has a fluorescent label. The amount of binding between the first and second components within the cell is determined by measuring a level of polarized and / or depolarized fluorescence emitted from within the biological cell.

[0011] The mentioned patent is a method for detecting intracellular binding reactions, especially DNA-DNA binding reactions and DNA-bound proteins, by the intracellular fluorescence polarization method. This method requires fluorescent staining to detect target molecules and is determined based on the measurement of fluorescence light. In contrast, the claimed invention does not require the addition of fluorescent labels, and the cells are preserved naturally, and the results are non-invasive. Also, the claimed device uses nanotechnology and artificial intelligence processing to image and analyze live molecular and intracellular reactions. The proposed patent examines a wide range of molecular reactions in living cells and, unlike the claimed invention, is not limited to a specific reaction.

[0012] CN101504370

[0013] Apparatus for simultaneous lossless detection of cell and extracellular matrix component

[0014] The invention relates to a device for performing synchronous nondestructive detection on intercellular and extracellular matrix components, which is characterized in that light emitted by a laser is incident onto a scanning mirror group of a scanner through a dichroic beam splitter, and light beams which pass through the scanning mirror group are focused on a detected sample by an objective lens; two-photon excited fluorescence and a secondary harmonic signal produced by the laser and the detected sample reversely passes through the same objective lens to be collected, then are incident onto a half transparent and half reflecting mirror by the scanning mirror group and the dichroic beam splitter; and emission signal light consisting of the two-photon excited fluorescence and the secondary harmonic signal is divided into two paths, the two paths are converted into electric signals through a filter plate and a photomuitplier and are connected to an input end of a computer, and finally the computer synchronously displays micro-structures of cells, elastic fibers and collagenous fibers. The device can perform high-resolution, high-contrast, nondestructive, quick and real-time detection on the microstructures of the cells, the elastic fibers and the collagenous fibers at molecular level.

[0015] The said invention is based on fluorescence methods based on two-photon excitation and second harmonic generation, and relies on fluorescence labeling, and uses high-intensity laser light, which in some cases may affect cells, and this invention is more for detecting the microstructure of cells, elastic fibers and collagen fibers at the molecular level. However, the claimed invention emphasizes dye-free imaging and uses nanotechnology and artificial intelligence processing to image, record and analyze intracellular and molecular reactions in real time, and provides a non-invasive function that examines cells in their natural state.

[0016] This invention is a device that includes a system which provides the ability to image live cells and instantly analyze intracellular reactions without the need for staining or chemical changes in the cells. This device uses an advanced combination of nanosensor technologies, nanomicroscopes, and artificial intelligence processors and can simultaneously simulate, analyze, and image molecular reactions inside cells. This device helps researchers observe chemical reactions, biological processes, and molecular changes inside cells without making chemical changes or staining. In addition to the ability to analyze data, the device displays the results in real-time, visual and analytical form. It is also portable and does not require the involvement of animals for testing.

[0017] - Limitations of live and unstained imaging

[0018] Many existing devices require staining and sample preparation to observe intracellular reactions, which may affect the normal functioning of cells and distort the results. Many existing devices cannot image and analyze all intracellular reactions in real time without the need for chemical or fluorescence changes.

[0019] - Lack of immediate and simultaneous data analysis

[0020] In most existing technologies, such as fluorescence microscopes or electron microscopes, data analysis is time-consuming. These devices are not capable of immediate data processing or immediate analysis, and these delays may prevent rapid and accurate understanding of cellular reactions and prediction of drug effects.

[0021] - Inability to simulate cell environmental conditions

[0022] Most of the current devices cannot adjust and change environmental conditions (such as temperature, pH, oxygen, and nutrients) to examine different effects on cells. This restriction means that the results of the experiments cannot accurately simulate the reactions under different conditions.

[0023] - The need for predicted data instead of live data in other devices

[0024] In some molecular simulation and bioinformatics systems, the data and results are pre-estimated and may not be accurate enough. These types of simulations do not reflect real-world results and are dependent on historical data.Solution of Problem

[0025] Current devices in the field of cellular imaging usually require staining or fluorescence or are limited to simulating some molecular reactions. Unlike traditional methods that require long-term processing, this device can simulate and analyze intracellular reactions in real time, and it is simultaneously able to:

[0026] • Observe molecular reactions in cells without the need for staining.

[0027] • Analyze chemical and biological reactions in real time.

[0028] • Uses a combination of advanced technologies such as nanosensors, nanomicroscopes, and artificial intelligence to provide high accuracy and performance.

[0029] The main features of the device:

[0030] • Live imaging without the need for staining:

[0031] Nano sensors and nano microscopes simultaneously image the molecular reactions and changes of cells, without the need for staining or chemical changes.

[0032] • Real-time analysis and data processing with artificial intelligence:

[0033] Real-time data is processed by artificial intelligence and the analysis results are displayed immediately in the form of graphic models and analytical charts.

[0034] • Simulation and adjustment of cell environmental conditions:

[0035] The device has a cell biochamber that allows researchers to change the environmental conditions of the cells (such as temperature, pH, pressure, and nutrients) and observe the cell reactions under these different conditions.

[0036] • Using live data instead of theoretical predictions:

[0037] The device uses real and live data to simulate and analyze cell reactions. This feature allows for more accurate results.

[0038] Main parts of the device:

[0039] • Nanosensors:

[0040] Nanosensors are the core of the device. These sensors are designed to detect molecules and chemical changes at the nanoscale. Nanosensors can accurately simulate changes in the molecular composition of cells in real time and send information to the device. These sensors can accurately simulate chemical and biochemical reactions inside cells without physical or chemical changes.

[0041] • Nano-microscopes:

[0042] These microscopes are designed for precise, nanoscale imaging of intracellular structures. Nanomicroscopes are specifically capable of 3D imaging of various components of the cell, including the nucleus, mitochondria, cell membrane, and other organelles. These microscopes can provide high-resolution, live images of cells and intracellular processes without the need for dyes or fluorescence.

[0043] •Touchscreen:

[0044] On the front of the device, there is a touch screen that allows the user to view data and control various settings. This screen should be colorful and high-resolution to display the visual results.

[0045] • AI processor:

[0046] The AI processor, which acts as the central unit of the device, automatically analyzes the data and images received from nano sensors and microscopes. Artificial intelligence algorithms are used to simulate molecular patterns and accurately analyze cellular reactions. The results obtained are displayed visually and analytically in the form of graphs, charts, and 3D images for viewing on the device screen. This part of the device plays a fundamental role in analyzing data and providing accurate and conceptual results.

[0047] • Bioreactor Chamber:

[0048] The bioreactor chamber is the part of the device that provides suitable environmental conditions for cells. This part allows the control of temperature, pH, oxygen, and nutrients in the cellular environment. In this way, the device can simulate cellular reactions under different conditions, allowing researchers to study the effects of different environmental conditions on cells. This chamber must be made of resistant and transparent materials to allow for detailed observation of cells and intracellular processes through a nano-microscope. This chamber can also be used as a cell culture system. The bioreactor also prevents cells from being hurt by environmental changes and provides conditions similar to those in the body for their growth.

[0049] • Wireless Charging and High-Capacity Battery System:

[0050] The device is charged wirelessly and has a high-capacity battery that can work for a long time without the need to be connected to a power source. This feature allows the device to be used in field research environments and hospitals. The wireless charging system makes it convenient to use the device in different places and does not restrict the device's movement. (If necessary, the device can also be used with electricity.)

[0051] • Subsidiary equipment:

[0052] Wireless connection: To transfer data to databases or research applications, Wi-Fi or Bluetooth technologies must be used.

[0053] • Transparent laboratory tape:

[0054] Laboratory tapes can be placed on the front of the device to hold cells.Advantage Effects of the Invention

[0055] 1- No need for dyes or external chemicals

[0056] 2- Real-time and ultra-sensitive imaging

[0057] 3- Combination of nanophotonic technologies, biosensors, and artificial intelligence processing

[0058] 4- Reduce the need for animal and human experiments

[0059] 5- Instant analysis of live and real data

[0060] 6- Possibility of identifying early signs of cancer, neurological and metabolic diseases by examining the molecular reactions of cells.

[0061] 7- Investigating patients' cellular response to drugs to select the most effective possible treatment.

[0062] 8- Possibility of monitoring patients' cells during treatment and observing the body's reactions to drugs.

[0063] 9- Use in cell engineering and biotherapeutics

[0064] 10- Helping scientists and researchers discover new drugs and new treatment methods faster.

[0065] 11- Reducing testing time

[0066] Shows the device from a view in which all the main parts are shown.

[0067] Displays the inner pieces of the system.

[0068] Presents the device from a side view.

[0069] Depicts the processor of the system.

[0070] ] Presents the device from a view in which all the main parts are shown:

[0071] 1- The body of the device, which is designed argonomically and small in size for easy transportation.

[0072] 2- Nanosensor and microscope for precise imaging. Nanosensor and microscope are directly connected to the chamber to observe and analyze reactions under different environmental conditions simultaneously.

[0073] 3- Bio reactor chamber that is accessible for placing samples and adjusting environmental conditions such as temperature, pH, and oxygen. This section is designed to have ventilation and temperature control systems to maintain cells in optimal conditions. The chamber has a transparent cover to allow for easy imaging of cells through nanomicroscopes, and its cover is durable and sterilizable to prevent contamination.

[0074] 4- Hand placing sites above the transparent cover of the chamber for the times when more sterilization is needed to work with cells and tissues.

[0075] 5- The handle to open and close the chamber.

[0076] 6- A transparent tape for cell retention

[0077] 7- The wireless connection and input ports for external data are located on the side of the device.

[0078] 8- The touch screen on the front of the device displays the results of data analysis and images.

[0079] 9- Cable plug so the device could work with electrical power too.

[0080] Displays the inner pieces of the system which are:

[0081] 10- The circuit board and AI processor

[0082] 11- Batteries for wireless mode of the device

[0083] Shows the device from the side view in which the plug and the inlet ports are visible to see.

[0084] Displays the circuit board of the device that connects electronic components:

[0085] 7- The circuit board to balance the nano sensors and the nano microscope, with AI processor and other digital fragments of the system. It manages the signals of the sensors and their transformation, the light source, image processing, and transferring data to be displayed on the screen, and also the cloud server. The circuit functions beside the Artificial intelligence processor in this device.

[0086] 10- The portsExamples

[0087] - Application in the development of anticancer drugs:

[0088] One of the major challenges in the development of anticancer drugs is to study the effect of these drugs on cancer cells without harming healthy cells. Traditional methods, such as fluorescence staining and imaging with conventional microscopes, may affect the structure and function of the cells, making the results unreliable. Also, these methods require a lot of time and cost.

[0089] - Using this device, researchers can:

[0090] 1- Study the process of the effect of new drugs on living cells in real time, without the need to stain or change the structure of the cells.

[0091] 2- Analyze the behavior of cancer cells under the influence of drugs and see whether the drug kills cancer cells.

[0092] 3- Distinguish healthy and cancerous cells, because the device can non-invasively examine subtle changes in intracellular reactions.

[0093] This invention can be of great use in the following fields:

[0094] 1- Pharmaceutical industry and drug development

[0095] Investigating the effect of drugs on living cells:

[0096] • Possibility of observing cellular reactions to drug compounds in real time.

[0097] • Reducing the need for long-term animal and clinical trials by simulating cellular reactions.

[0098]

[0099] 2- Increasing the speed of drug screening:

[0100] • Helping to develop new drugs faster by analyzing biological reactions to active ingredients.

[0101] • Reducing research and development costs in the pharmaceutical industry.

[0102]

[0103] 3- Biotechnology and bioengineering

[0104] Cell and tissue engineering:

[0105] • Analyzing the growth and differentiation of stem cells without the need for external intervention.

[0106] • Quality control in cell cultures and engineered tissues for advanced medical treatments.

[0107]

[0108] 4- Monitoring biological processes in the biotechnology industry:

[0109] • Monitoring biochemical changes in industrial culture media to optimize the production of biological products.

[0110] • Increasing efficiency in the production of recombinant proteins, antibodies and vaccines.

[0111]

[0112] 5- Medical diagnostics and advanced therapies

[0113] Prediction and diagnosis of cellular diseases:

[0114] • Early diagnosis of cancers, neurological diseases and metabolic disorders by examining cellular changes.

[0115] • Providing non-invasive methods for examining biological samples in medical diagnostics.

[0116] 6- Personalized medicine:

[0117] • Examining individual patient responses to drugs to select the most effective possible treatment.

[0118] • Cellular monitoring of patients to track the recovery process in advanced treatments.

Claims

1.A real-time bioimaging system capable of observing and analyzing intracellular reactions in living cells with nanometer precision without the need for staining.2.According to claim 1, a nanophotonic microscope is embedded in this device that utilizes plasma nanoparticle technology and quantum imaging to enable the observation of molecular structures without the need for fluorescence agents.3.According to claim 1, there are ultra-sensitive nanobiological sensors on the system that detect biological, chemical, and electrophysiological changes in cells without direct contact and in a non-invasive manner.4.According to claims 2 and 3, an artificial intelligence-based image processing system is available to automatically analyze the data collected from sensors and the microscope and identify biological patterns for disease diagnosis.5.According to claim 1, an ultra-sensitive spectroscopy technology with nanophotonic imaging is combined, which allows the observation of molecular interactions and signaling pathways inside the cell without the need for invasive methods.6.According to claim 1, this device is portable that permits for use in clinical, hospital, laboratory, and outside of research centers.7.According to claim 1, the system connects to databases and cloud computing systems to let users store, update, and compare imaging and cellular analysis data.8.According to claim 1, the system examines intracellular responses to drugs and biological compounds in real time, which leads to an increase in the speed of pharmaceutical research and the development of new therapeutic methods.9.According to claim 1, there is no need to have laboratory animals in biological studies by operating this system to investigate environmental, chemical and drug effects on living cells.10.According to claim 1, this system provides accurate information about structural, mechanical and biochemical changes in cells without the need for complex sample preparation, which results in reduced research time and costs.

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