Method for manufacturing leather sheet using mushroom mycelium

Abstract

The present invention relates to a method for manufacturing a mushroom mycelium leather sheet using shiitake mycelium and relates to a method for manufacturing a mushroom mycelium leather sheet that exhibits color and texture similar to animal leather by inducing the curing phenomenon of a shiitake mushroom.

Description

Method for manufacturing a leather seat using mushroom mycelium

[0001] The present invention relates to a method for manufacturing a leather sheet using mushroom mycelium, and more specifically, to a method for manufacturing a mushroom mycelium leather sheet that exhibits a color and texture similar to animal leather by utilizing the curing phenomenon of shiitake mushrooms.

[0002] While the global preference for and demand for leather are increasing, the demand for alternative leather (synthetic leather) is rising as consumers' standards for ethical consumption also increase, as the leather-producing process does not involve extensive slaughter or environmental destruction.

[0003] However, due to the significant difference in texture between artificial leather and natural leather, the molding process to meet consumer expectations has become more complex, leading to an increase in processing molding steps involving dyeing and bonding. Consequently, manufacturing costs are rising rapidly, and the industry is facing various problems, including serious environmental pollution throughout the entire process, from the use of petroleum-based polymers in manufacturing to their disposal as industrial waste due to their poor biodegradability and inability to be recycled.

[0004] Under the circumstances described above, research is actively underway on the manufacture of leather using materials such as plant fibers, mushrooms, and silicone that can replace natural leather, such as animal hides.

[0005] Leather sheets made using mushroom mycelium are formed into final products after undergoing a dyeing process through immersion or spraying by dissolving pigments or dyes in water or a solvent to produce a color and texture similar to animal leather.

[0006] However, when pigments or dyes are used as described above to achieve colors similar to animal hides, there are concerns regarding environmental pollution and human safety due to the use of various chemicals, and it is also undesirable in terms of cost as it requires additional processes.

[0007] Therefore, there is a need to develop a new method to achieve the natural color of animal leather without using pigments or dyes in leather sheets made from mushroom mycelium.

[0008] Accordingly, the inventors of the present invention have developed a method for manufacturing a mycelial leather sheet with improved physical properties such as flexibility and strength, while imparting a color and texture similar to real animal leather without the addition of a separate dye by appropriately adjusting the culture conditions of shiitake mushroom mycelium.

[0009] The present invention aims to provide a method for manufacturing a mycelial leather sheet that can stimulate purchasing desire among consumers by having vivid leather color and texture through the curing phenomenon of shiitake mushroom mycelium.

[0010] In addition, the present invention aims to provide a method for manufacturing an eco-friendly mycelial leather sheet that has appropriate flexibility and excellent durability by laminating two types of mushroom mycelial leather sheets.

[0011] The technical problems of the present invention are not limited to those mentioned above, and other unmentioned technical problems will be clearly understood by those skilled in the art from the description below.

[0012] According to an embodiment of the present invention, a method for manufacturing a leather sheet using shiitake mushroom mycelium is provided, comprising: (a) a step of dark culturing shiitake mushroom mycelium using a solid medium; (b) a step of aging shiitake mushroom mycelium by culturing the shiitake mushroom mycelium dark-cultured in step (a) in light; and (c) a step of manufacturing a shiitake mushroom mycelium leather sheet by compressing the shiitake mushroom mycelium sheet aged in step (b).

[0013] In addition, the solid medium of step (a) above may comprise 65 to 85 weight% of sawdust, 15 to 21 weight% of rice bran, 3 to 7 weight% of pectin, and 1 to 3 weight% of calcium carbonate based on the total weight of the solid medium.

[0014] In addition, the solid medium of step (a) above may additionally include a gum thickener.

[0015] In addition, the light culture of step (b) above can be performed under conditions of irradiating light of 100 to 32,000 lux.

[0016] In addition, the light culture of step (b) above can be performed at a temperature of 20 to 30°C.

[0017] In addition, the method for manufacturing the mushroom mycelium leather sheet may further include the step of (d) laminating the reishi mushroom mycelium leather sheet onto one side of the shiitake mushroom mycelium leather sheet.

[0018] In addition, the thickness of the shiitake mushroom mycelium leather sheet may be 0.1 to 5 mm, and the thickness of the reishi mushroom mycelium leather sheet may be 0.1 to 10 mm.

[0019] In addition, the shiitake mushroom mycelium leather sheet and the reishi mushroom mycelium leather sheet can be laminated at a thickness ratio of 1:1.5 to 5.

[0020] In addition, according to another embodiment of the present invention, a mushroom mycelium leather sheet produced by the above manufacturing method is provided.

[0021] The mushroom mycelium leather sheet produced according to the present invention has excellent durability and achieves the vivid color and texture of leather, providing a satisfying appearance similar to animal leather, which can stimulate consumers' desire to purchase.

[0022] In addition, the above mushroom mycelium leather sheet does not correspond to leather derived from animals, so no ethical issues arise regarding leather acquisition.

[0023] In addition, the above mushroom mycelium leather sheet can achieve natural colors and textures without using separate pigments or dyes, allowing for the manufacture of leather sheets in an eco-friendly manner.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by a skilled expert in the art to which the present invention pertains. In general, the nomenclature used herein is well known and commonly used in the art. Furthermore, in describing embodiments of the present invention, detailed descriptions of related known components or functions are omitted if it is determined that such detailed descriptions would hinder understanding of the embodiments of the present invention. Additionally, while embodiments of the present invention will be described below, the technical concept of the present invention is not limited or restricted thereto and can be modified and implemented in various ways by those skilled in the art.

[0025] In this specification, when a part is described as including a certain component, it means that, unless specifically stated otherwise, it does not exclude other components but may include additional components. In this specification, the term "and / or" includes a combination of a plurality of related items or any one of a plurality of related items.

[0026] According to an embodiment of the present invention, a method for manufacturing a mushroom mycelium leather sheet comprises: (a) a step of dark culturing shiitake mushroom mycelium using a solid medium; (b) a step of aging the shiitake mushroom mycelium by culturing the shiitake mushroom mycelium dark-cultured in step (a) in light; and (c) a step of manufacturing a shiitake mushroom mycelium leather sheet by compressing the aged shiitake mushroom mycelium sheet from step (b); thereby providing a method for manufacturing a mushroom mycelium leather sheet that is externally similar to animal leather by realizing vivid color and texture and has improved durability.

[0027] In the present invention, the term “mushroom mycelium” refers to aerial hyphae, biofilm hyphae, and penetrative hyphae; in a narrow sense, it refers to biofilm hyphae, and in a broad sense, it is a concept that includes aerial hyphae and biofilm hyphae.

[0028] The cell wall of mushroom mycelium has a structure in which polysaccharides (α-1,3-glucan) and glycoproteins (mainly mannan or galactomannoproteins) are connected in an amorphous gel-like matrix on a membrane woven with microfibers of chitin and β-1,3-glucan. Chitin and β-1,3-glucan are key components that maintain the structure of the cell wall, and in particular, chitin is a very important component for the mechanical strength of the mycelial fibers.

[0029] Growing mushroom mycelia are nutrient-dependent and grow by absorbing nutrients while expanding their tips. After the expansion of the mycelia, tips may form at the cell walls, leading to branching, which extends in a continuous and characteristic radial pattern. These branches elongate in various directions, resulting in numerous fusions between the mycelia. From a material perspective, the fusion of mycelia exhibits uniformity, while from a mechanical perspective, the formation of a fine mesh of mycelia increases mechanical strength. The mushroom mycelium consists of intertwined hyphae forming a network layer and possesses a microstructure similar to the dermis of animal hide; however, due to the low density of the mycelia, there are limitations to its durability and strength.

[0030] First, prior to the cancer culture of step (a) above, a step of filling a culture vessel with solid medium and sterilizing it in a sterilization chamber may be performed.

[0031] Specifically, the sterilization can be performed by placing the solid medium in a sterilization chamber and sterilizing it at 100 to 135°C for 2 to 3 hours to maximize the sterilization effect, and the sterilized solid medium can be cooled to room temperature in the sterilization chamber.

[0032] Next, the sterilized solid medium is cooled to 18°C ​​or lower, then shiitake mushroom spawn is inoculated in a sterile inoculation room, and the spawn is cultured in the dark in a culture room to cultivate shiitake mushroom mycelium [(a) step].

[0033] In one embodiment, the solid medium may be used as a culture substrate that serves as a food source for the mycelium, and may be a mixed medium comprising 65 to 85 weight% of sawdust, 15 to 21 weight% of rice bran, 3 to 7 weight% of pectin, and 1 to 3 weight% of calcium carbonate based on the total weight of the solid medium.

[0034] In one embodiment, the solid medium comprises sawdust as a main component and may contain 65 to 85 weight percent of sawdust, specifically 70 to 80 weight percent of sawdust.

[0035] In one embodiment, the solid medium may contain 15 to 21 weight% of rice bran as a carbon source and 3 to 7 weight% of pectin as a nitrogen source, specifically 16 to 20 weight% of rice bran and 4 to 6 weight% of pectin.

[0036] When rice bran and pectin are included in the above content range in the solid medium, the mycelium not only grows actively on the surface of the medium but also shows a high-density growth pattern by intertwining tightly with each other.

[0037] In one embodiment, the solid medium may additionally include any one of a nitrogen source selected from dextrose, yeast extract, malt extract, and ammonium nitrate in addition to pectin.

[0038] In one embodiment, the solid medium may contain 1 to 3 weight percent of calcium carbonate, specifically 1.5 to 2.5 weight percent of calcium carbonate. The calcium carbonate acts as a buffer to maintain the pH of the medium neutrally in the mixed medium, thereby promoting mycelial growth. Accordingly, the calcium carbonate promotes the growth of mushroom mycelia, thereby forming a dense fine mesh of mycelia, which can improve the elongation and strength of the mycelial leather sheet.

[0039] In one embodiment, the solid medium may additionally include a gum thickener. By further including the gum thickener, the appearance of the mycelial sheet can be grown more uniformly.

[0040] In one embodiment, the gum thickener may include one or more of glucomannan, agar, gelatin, guar gum, carrageenan, and xanthan gum, and preferably may include agar, but is not limited thereto.

[0041] When the above solid medium contains agar as a gum thickener, it not only helps the mycelial sheet grow uniformly, but also plays a role in controlling the mycelial sheet so that excessive browning does not occur and the physical properties of the mycelial sheet do not deteriorate.

[0042] In one embodiment, the gum thickener may comprise 2 to 30 weight percent based on the total weight of the solid medium, specifically 5 to 20 weight percent, and more specifically 5 to 15 weight percent. The amount of the gum thickener can be appropriately set by a person skilled in the art within the above range according to the range of flexibility and strength that the final mycelial leather sheet is to optimally achieve.

[0043] In one embodiment, the spawn inoculated into the solid medium may be any one of liquid spawn, sawdust spawn, grain spawn, etc.

[0044] In one embodiment, the dark culture of step (a) of the manufacturing method of the present invention may be performed under conditions of irradiating light of 0 to 50 lux. If light of 50 lux or more is irradiated, the dark culture may not proceed properly, and the surface of the mycelium may become rough or difficult to press, which may reduce the processability of manufacturing a mycelium leather sheet.

[0045] In one embodiment, the dark culture of step (a) of the manufacturing method of the present invention can be performed at a temperature of 20 to 30°C. Specifically, shiitake mushroom mycelium can be cultured by dark culturing the spawn for 10 to 30 days in a culture room where the carbon dioxide concentration is less than 2000 ppm and the temperature is 20 to 30°C under conditions of irradiating light of 0 to 50 lux. During dark culture, the mycelium spreads downward from the top of the solid medium where it was inoculated, forming white shiitake mushroom mycelium. The inventors have confirmed that when dark culture is performed under the above conditions, it is possible to produce a mycelium sheet that has excellent processability for manufacturing a mycelium leather sheet, while also achieving a texture and durability similar to animal leather.

[0046] When the dark culture of the shiitake mushroom mycelium is completed through the above step (a), the shiitake mushroom mycelium is matured by light culture under conditions of higher light intensity than the dark culture [step (b)].

[0047] When conducting light culture of the above shiitake mushroom mycelium, a curing phenomenon can be induced starting from the surface of the mycelium irradiated with light, thereby enabling the realization of a natural color similar to that of animal skin.

[0048] In this specification, the term “curing phenomenon” refers to a phenomenon in which mycelium changes color as it undergoes an oxidation reaction with oxygen in the air. By appropriately controlling the curing phenomenon, a color similar to animal leather can be achieved and excellent durability can be maintained, so the color of the mycelium leather sheet can be naturally achieved without using separate dyes or pigments, making it an eco-friendly method.

[0049] When mycelium is exposed to conditions where appropriate temperature and light intensity are set, color is imparted to the surface of the mycelium, inducing a curing (browning) phenomenon that changes to a color similar to that of animal hide, thereby allowing the natural color of leather to be expressed.

[0050] In one embodiment, the light culture of the dark-cultured mycelium in step (b) of the manufacturing method of the present invention can be performed under conditions of irradiating light of 100 to 32,000 lux, specifically at 100 to 25,000 lux, more specifically at 100 to 10,000 lux, even more specifically at 100 to 5,000 lux, and most specifically at 100 to 2,500 lux.

[0051] If the light intensity of the light reaction is less than 100 lux, the curing phenomenon may not occur uniformly or may proceed at a significantly slow speed, resulting in poor processability; if it exceeds 32,000 lux, the curing phenomenon may proceed excessively, causing water to be generated due to side reactions, which may degrade the product quality as leather or result in unnatural colors; and if the mycelial tissue is damaged, the mechanical properties of the leather sheet may be significantly degraded, making it vulnerable to physical impact, or the mycelial tissue may not grow enough to be manufactured into a leather sheet.

[0052] In one embodiment, the light culture of step (b) of the manufacturing method of the present invention can be performed at a temperature of 20 to 30°C, and specifically, the shiitake mushroom mycelium can be light cultured for 30 to 50 days by irradiating light of 100 to 32,000 lux, 100 to 25,000 lux, 100 to 10,000 lux, 100 to 5,000 lux, or 100 to 2,500 lux at a temperature of 20 to 30°C.

[0053] The inventors have confirmed that when light culture is carried out under the above conditions, the mycelium and / or mycelium sheet can achieve a color similar to animal leather without becoming excessively flexible, thereby enabling the production of a mycelium sheet with excellent durability.

[0054] A “shiitake mushroom mycelium sheet” can be obtained by separating the entire or a portion of the top of a shiitake mushroom mycelium bed in which cultivation is complete.

[0055] In one embodiment, once mycelial culture is completed, a plasticizer treatment may be performed to improve the physical properties of the leather prior to the compression process of the shiitake mushroom mycelial sheet.

[0056] In one embodiment, the plasticizer treatment can be performed while supplying air bubbles to the mushroom mycelium sheet. That is, the mushroom mycelium sheet is immersed in the plasticizer, and air bubbles are supplied within the plasticizer, so that the air bubbles move toward the mushroom mycelium sheet and strike the mushroom mycelium sheet. Accordingly, the entanglement structure between the mycelium is loosened, and the action of the plasticizer can be facilitated.

[0057] In one embodiment, the plasticizer may include at least one of propylene carbonate and ethyl alcohol, but is not limited thereto.

[0058] Next, a shiitake mushroom mycelium sheet separated from the shiitake mushroom mycelium bed, where cultivation is completed through step (b) above, is compressed to produce a shiitake mushroom mycelium leather sheet [step (c)].

[0059] In one embodiment, the compression process of the shiitake mushroom mycelium sheet may be performed as a room temperature compression process or a heat compression process, and during the compression process, the moisture content of the shiitake mushroom mycelium sheet may be reduced and the thickness of the shiitake mushroom mycelium sheet may be reduced. In addition, the density of the shiitake mushroom mycelium sheet may be increased and the strength of the shiitake mushroom mycelium sheet may be increased.

[0060] In one embodiment, the compression process may be such that a shiitake mushroom mycelium sheet is placed between a pair of compression plates, and as force is applied to at least one of the pair of compression plates, the mycelium sheet is compressed to form a shiitake mushroom mycelium leather sheet having higher density and strength.

[0061] Specifically, the above room temperature pressing process may be carried out at 18 to 30°C at 1000 to 3000 psi for 10 to 60 minutes, but is not limited thereto.

[0062] Specifically, the above heat pressing process may be carried out at 100 to 120°C at 500 to 1000 psi for 5 to 15 minutes, but is not limited thereto.

[0063] In one embodiment, the method for manufacturing a mushroom mycelium leather sheet of the present invention may further include the step of laminating a Ganoderma mycelium leather sheet onto one side of a shiitake mushroom mycelium leather sheet manufactured by the compression process of step (c) [step (d)].

[0064] In one embodiment, when the Reishi mushroom mycelium leather sheet is laminated to the shiitake mushroom mycelium leather sheet manufactured according to the manufacturing method of the present invention, it can be manufactured into a mushroom mycelium leather sheet having appropriate flexibility and durability and a color similar to animal leather.

[0065] The above-mentioned Reishi mushroom mycelium grows relatively quickly, which can lower the production cost of leather, and also has high durability. Therefore, the Reishi mushroom mycelium leather sheet is suitable as a laminated material to reinforce the physical properties of the Shiitake mushroom mycelium leather sheet.

[0066] In one embodiment, the reishi mushroom mycelium leather sheet can be manufactured by a known method, specifically by the same method as the manufacturing method of the shiitake mushroom mycelium leather sheet.

[0067] In one embodiment, a Ganoderma lucidum mycelium leather sheet can be laminated to one side of the outer surface of a shiitake mushroom mycelium leather sheet that has undergone an aging step and has relatively less discoloration, and the lamination process can be performed using a roller and an eco-friendly water-soluble adhesive.

[0068] In one embodiment, the thickness of the shiitake mushroom mycelium leather sheet in step (d) may be 0.1 to 5 mm, and the thickness of the reishi mushroom mycelium leather sheet may be 0.1 to 10 mm.

[0069] In one embodiment, the shiitake mushroom mycelium leather sheet of step (d) and the reishi mushroom mycelium leather sheet may be laminated with a thickness ratio of 1:1.5 to 5. By having the thickness ratio, the flexibility, strength, and durability of the shiitake mushroom mycelium leather sheet of the present invention may be further enhanced.

[0070] Finally, a coating layer is formed on the other side of the shiitake mushroom mycelium leather sheet to improve moisture resistance and durability of the mycelium leather sheet and to make the leather-like color appear more vivid [(e) step].

[0071] In one embodiment, the step (e) may be performed after step (c) or after step (d) to complete the shiitake mushroom mycelium leather sheet of the present invention.

[0072] Any pigment may be used as a coating layer material on one side of the above shiitake mushroom mycelium leather sheet, and the coating may be performed by a spray coating method in which a certain amount of pigment is sprayed, or by a roller coating method, but is not limited thereto.

[0073] According to another embodiment of the present invention, a mycelium leather sheet manufactured by the method for manufacturing a shiitake mushroom mycelium leather sheet of the present invention is provided. The mycelium leather sheet has excellent durability and can stimulate consumers' desire to purchase by realizing vivid colors and textures similar to animal leather.

[0074] In the following, examples and experimental examples are presented to further explain the present invention in more detail, but the present invention is not limited thereto.

[0075] Experimental Example 1. Comparative experiment according to solid medium

[0076] (1) Preparation of Examples and Comparative Examples

[0077] 1) Preparation of a shiitake mushroom mycelium bed

[0078] A solid medium containing 75% by weight of sawdust, 18% by weight of rice bran, 5% by weight of pectin, and 2% by weight of calcium carbonate based on the total weight of the solid medium was placed in a medium container and sterilized in a sterilization room, and then the shiitake mushroom mycelium bed of Example 1 was grown under the dark culture and light culture conditions listed in Table 1.

[0079] Temperature (°C) Light Intensity (Lux) Duration (Days) Dark Culture 20 ~ 300 ~ 50 20 People Culture 20 ~ 30 100 ~ 200 40

[0080] In Example 2 and Comparative Examples 1 to 3, a bed of shiitake mushroom mycelium was grown using a mixed medium containing the ingredients listed in Table 2, and dark culture and light culture were carried out under the same conditions as in Example 1.

[0081] Example Comparative Example 1 2123 Sawdust 7570757575 Rice bran 1815-1818 Pectin 53.55-5 Calcium carbonate 21.522- Agar-101852

[0082] 2) Manufacture of shiitake mushroom mycelium leather sheets

[0083] A shiitake mushroom mycelium sheet isolated from a cultured mycelium bed was dried at room temperature and compressed at 25°C at a pressure of 2000 psi for 30 minutes to produce a 1 mm thick shiitake mushroom mycelium leather sheet.

[0084] 3) Color development evaluation

[0085] Samples of shiitake mushroom mycelium leather sheets prepared according to Examples 1 and 2 and Comparative Examples 1 to 3 were measured for lightness (L) and color (a,b) using a colorimeter (Minolta CM-2500d), and the results are shown in Table 3.

[0086] Example Comparative Example 1 21 23L (Lightness) 70.16 9.28 0.18 2.78 3.2a (Color Coordinates) 2.2 2.3 2.12 2.0 2.2b (Color Coordinates) 20.11 9.5 19.8 20.2 19.5

[0087] 4) Evaluation of elongation

[0088] Shiitake mushroom mycelium leather sheets prepared according to Examples 1 and 2 and Comparative Examples 1 to 3 were cut to a size of 100 mm in length and 10 mm in width to measure the thickness of the sample, and the sample was fixed to a Universal Testing Machine (UTM) and stretched at a speed of 100 mm / min until the sample broke to measure the elongation rate, and the results are shown in Table 4.

[0089] Example Comparative Example 1 2 1 2 3 Elongation (%) 6 5 6 8 5 3 5 6 5 5

[0090] According to Tables 3 and 4 above, the shiitake mushroom mycelium leather sheets of Examples 1 and 2, prepared using mycelium grown in a solid medium containing sawdust, rice bran, pectin, and calcium carbonate, were found to have developed a dark brown color and possessed excellent elongation, confirming that they possessed mechanical properties suitable for use as leather. In particular, in the case of Example 2, which included agar as a gum thickener in the solid medium, it was confirmed that the surface of the leather sheet was smoother and achieved a softer texture compared to Example 1. On the other hand, the shiitake mushroom mycelium leather sheets of Comparative Examples 1 to 3, prepared using mycelium grown in a solid medium that did not contain one of the components among rice bran, pectin, and calcium carbonate, showed uneven discoloration overall; furthermore, the degree of discoloration was insufficient, resulting in high brightness and reduced elongation. Through the measurement results of the above examples and comparative examples, it was confirmed that the mushroom mycelium leather sheet of the present invention achieves a color and texture similar to animal leather without using separate pigments or dyes, and that its mechanical properties are also suitable for use as a leather sheet.

[0091] Therefore, it was confirmed that including sawdust, rice bran, pectin, and calcium carbonate in appropriate amounts in a solid medium is an important component for producing leather sheets with vivid color and excellent mechanical properties by promoting the growth of shiitake mushroom mycelium.

[0092] Experimental Example 2. Comparative experiment according to light culture conditions

[0093] (1) Preparation of comparative example

[0094] 1) Preparation of a shiitake mushroom mycelium bed

[0095] Comparative Examples 4 and 5 used the same solid medium as Example 1, and grew shiitake mushroom mycelium beds by changing only the light culture conditions from Example 1 as described in Table 5.

[0096] Temperature (°C) Light Intensity (Lux) Period (Days) Example 1 20 ~ 30 100 ~ 200 40 Comparative Example 4 20 ~ 30 50 ~ 90 40 Comparative Example 5 20 ~ 30 35,000 ~ 40,000 40

[0097] 2) Manufacture of shiitake mushroom mycelium leather sheets

[0098] A mycelium sheet isolated from a cultured mycelium bed was dried at room temperature and compressed at 25°C at a pressure of 2000 psi for 30 minutes to produce a 1 mm thick shiitake mushroom mycelium leather sheet.

[0099] 3) Color development evaluation

[0100] The lightness and color of the shiitake mushroom mycelium leather sheets prepared according to Comparative Examples 4 and 5 were measured in the same manner as in Experimental Example 1, and the results are shown in Table 6.

[0101] Example Comparative Example 145L (Lightness) 70.18 0.15 0.1a (Color Coordinates) 2.2 1.10 2.2b (Color Coordinates) 20.14 80 20.1

[0102] 4) Evaluation of elongation

[0103] The elongation of the shiitake mushroom mycelium leather sheets prepared according to Comparative Examples 4 and 5 was measured in the same manner as in Experimental Example 1, and the results are shown in Table 7.

[0104] Example Comparative Example 145 Elongation 655529

[0105] According to Tables 6 and 7 above, in Comparative Example 4, where shiitake mushroom mycelium was cultured in bright light by irradiating it with 50 to 90 lux light, the elongation rate showed a value similar to Example 1, but discoloration did not occur sufficiently, so a color similar to animal leather did not appear. In addition, in Comparative Example 5, where shiitake mushroom mycelium was cultured in bright light by irradiating it with 35,000 to 40,000 lux light, it was confirmed that the elongation rate became excessively low and discoloration progressed excessively, resulting in a decrease in brightness.

[0106] Therefore, it was confirmed that controlling the light intensity during the light culture of shiitake mushroom mycelium is an important component in producing an eco-friendly leather sheet with an appearance similar to animal leather by allowing the curing phenomenon of the shiitake mushroom mycelium to proceed to an appropriate degree.

[0107] Experimental Example 3. Comparative experiment according to Ganoderma mycelium sheet thickness

[0108] (1) Preparation of Examples and Comparative Examples

[0109] 1) Preparation of a shiitake mushroom mycelium bed

[0110] Examples 3 and Comparative Examples 6 and 7 used the same solid medium as Example 1, and shiitake mushroom mycelium beds were grown under the dark culture and light culture conditions described in Experimental Example 1.

[0111] 2) Manufacture of composite mycelium leather sheets

[0112] A shiitake mushroom mycelium bed separated from a cultured mycelium bed was post-dried at room temperature and compressed at 25°C at a pressure of 2000 psi for 30 minutes to produce a shiitake mushroom mycelium leather sheet with a thickness of 0.5 mm. A reishi mushroom mycelium leather sheet having the thickness listed in Table 8 was laminated onto one side of the shiitake mushroom mycelium leather sheet with a thickness of 0.5 mm to produce the composite mycelium leather sheets of Example 3, Comparative Example 6, and Comparative Example 7.

[0113] Example Comparative Example 367 (A) Shiitake mushroom mycelium leather sheet thickness (μm) 0.5 0.5 0.5 (B) Reishi mushroom mycelium leather sheet thickness (μm) 1.5 0.5 3 (A) : (B) 1 : 3 1 : 11 : 6

[0114] 3) Color development evaluation

[0115] The lightness and color coordinates of the composite mycelial leather sheets prepared according to Example 3 and Comparative Examples 6 and 7 were measured in the same manner as in Experimental Example 1, and the results are shown in Table 9.

[0116] Example Comparative Example 367L (Lightness) 70.170.270.5a (Color Coordinates) 2.22.32.0b (Color Coordinates) 20.220.119.4

[0117] 4) Evaluation of elongation

[0118] The elongation of the composite mycelial leather sheets prepared according to Example 3 and Comparative Examples 6 and 7 was measured in the same manner as in Experimental Example 1, and the results are shown in Table 10.

[0119] Example Comparative Example 367 Elongation 846568

[0120] According to Tables 9 and 10 above, Example 3, in which the thickness ratio of the shiitake mushroom mycelium leather sheet to the reishi mushroom mycelium leather sheet is within an appropriate range, was confirmed to have developed a color similar to leather and excellent elongation. On the other hand, Comparative Example 6, in which the thickness ratio of the shiitake mushroom mycelium leather sheet to the reishi mushroom mycelium leather sheet is 1:1, was confirmed to have developed a leather-like color but a lower elongation compared to Example 3. In addition, Comparative Example 7, in which the thickness ratio of the shiitake mushroom mycelium leather sheet to the reishi mushroom mycelium leather sheet is 1:6, was also confirmed to have a similar degree of color development to the examples, but with a lower elongation compared to Example 3, resulting in weaker physical properties.

[0121] Therefore, it was confirmed that including shiitake mushroom mycelium leather sheet and reishi mushroom mycelium leather sheet in an appropriate thickness ratio is an important component for improving the durability of the leather sheet.

[0122] Foregoing, specific parts of the content of the present invention have been described in detail. It will be apparent to those skilled in the art that such specific descriptions are merely preferred embodiments and do not limit the scope of the present invention. Accordingly, the actual scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for manufacturing a leather seat using mushroom mycelium, (a) A step of dark culturing shiitake mushroom mycelium using a solid medium; (b) a step of maturing the shiitake mushroom mycelium by culturing the dark-cultured shiitake mushroom mycelium from step (a) in the light; and (c) a step of manufacturing a shiitake mushroom mycelium leather sheet by compressing the aged shiitake mushroom mycelium sheet of step (b) above; comprising a method for manufacturing a mushroom mycelium leather sheet.

2. In Paragraph 1, A method for manufacturing a mushroom mycelium leather sheet, wherein the solid medium of step (a) comprises 65 to 85 weight% sawdust, 15 to 21 weight% rice bran, 3 to 7 weight% pectin, and 1 to 3 weight% calcium carbonate based on the total weight of the solid medium.

3. In Paragraph 2, A method for manufacturing a mushroom mycelium leather sheet, wherein the solid culture medium of step (a) above further comprises a gum thickening agent.

4. In Paragraph 1, A method for manufacturing a mushroom mycelium leather sheet, wherein the light culture of step (b) above is performed under conditions of irradiating light of 100 to 32,000 lux.

5. In Paragraph 1, A method for manufacturing a mushroom mycelium leather sheet, wherein the light culture of step (b) above is performed at a temperature of 20 to 30°C.

6. In Paragraph 1, (d) a step of laminating a Ganoderma mycelium leather sheet onto one side of the shiitake mushroom mycelium leather sheet; further comprising a method for manufacturing a mushroom mycelium leather sheet.

7. In Paragraph 6, The above shiitake mushroom mycelium leather sheet has a thickness of 0.1 to 5 mm, and A method for manufacturing a mushroom mycelium leather sheet, characterized in that the above-mentioned reishi mycelium leather sheet has a thickness of 0.1 to 10 mm.

8. In Paragraph 6, A method for manufacturing a mushroom mycelium leather sheet, wherein the shiitake mushroom mycelium leather sheet and the reishi mushroom mycelium leather sheet have a thickness ratio of 1:1.5 to 5.

9. A mushroom mycelium leather sheet manufactured by the manufacturing method of any one of claims 1 to 8.