Nutraceutical formulation containing blatta orientalis for fattening animals

Abstract

The present invention relates to an anabolic nutraceutical formulation that offers, among other improvements : - feed conversion, favoring among others the following points : - weight gain with lower food consumption; - the animals reach the target weight set by the producer early; - a natural product, dosed in tiny quantities of active substance; - the anabolic nutraceutical contains Blatta orientalis; - non-toxic for the animal; - non-toxic for the consumer of this meat; - the meat remains adequately hydrated at the core; - the meat remains palatable, tender, succulent and soft; - administration is easy and occasional; - the consumption of the anabolic formulation optimizes the absorption of the nutrients in the food, as well as tissue oxygenation and, at the same time, increases blood flow; - the distribution of lean tissue is greater; - it improves animal health, which means less morbidity and mortality, resulting in savings for livestock producers; - the breeding mothers remain in optimal body condition during the lactation stage.

Description

[0001] NUTRACEUTICAL FORMULATION CONTAINING Blatta orientalis FOR

[0002] FATTENING ANIMALS

[0003] TECHNICAL FIELD OF THE INVENTION

[0004] The present invention relates to a novel nutraceutical formulation, hereinafter referred to as an anabolic nutraceutical, for fattening animals intended for human consumption, which aims to improve meat production efficiently while avoiding the use of synthetic chemical substances. The object of this invention is to offer the livestock industry an anabolic product in powder form with a formula different from those currently available on the market.

[0005] BACKGROUND OF THE INVENTION

[0006] Although a food additive is defined as a product or substance that is included in the formulation of the diet, at a low level of inclusion increasing the nutritional quality of the food and the vitality of the animal; it is not used for its nutritional value since it is assimilated in small quantities, having a high cost (Ravindran, 2010; Rebollar et al., 2015).

[0007] It is important to note that there is a regulation that evaluates and authorizes the marketing and use of feed additives, which guarantees protection of human health, animal health, and welfare (EC No. 1831 / 2003). These additives must favorably influence the production and consumption of sheep meat in Mexico by improving the characteristics of the feed and animal product, have no negative environmental impacts, meet the animal's nutritional needs, not disrupt the rumen microbiota, and have a coccidiostatic or histomonostatic effect (Carro et al., 2006). In Mexico, the Federal Commission for Protection against Sanitary Risks (COFEPRIS) is responsible for the regulations governing the use of feed additives.

[0008] Feed additives are classified according to their function as antioxidants, colorants, emulsifiers and flavor stabilizers, solvents, glazing agents, sweeteners, preservatives, thickeners, and bulking agents (Velázquez et al., 2019). Furthermore, the EC regulation categorizes additives as: a) technological, which is any substance added to feed, such as preservatives, antioxidants, emulsifiers, stabilizers, thickeners, and gelling agents; b) organoleptic, which is a substance added to feed to improve its properties, whether visual, aromatic, or in terms of consistency; c) nutritional, such as vitamins, chemical substances with similar effects, amino acids, salts, and urea; d) zootechnical, which are used to obtain a positive effect on animal productivity, such as intestinal flora stabilizers; and e) coccidiostatic and histomonostatic.

[0009] In this sense, a growth promoter is a product that increases an animal's weight gain. Not all growth promoters have the same effect, as this depends on their characteristics, chemical structure, and function at the cellular level. Some authors state that they improve productivity, product quality, reduce the release of nitrogenous waste into the environment, improve feed efficiency in livestock, and decrease pressure on fragile ecosystems (Sillence, 2004; Strydom et al., 2009).

[0010] The use of anabolic agents with non-hormonal activity is one of the non-genetic methods to modify the growth potential of animals.

[0011] An anabolic steroid is defined as any compound or mixture of compounds that affects an animal's metabolic function to increase the amount of body protein. Considering the above, some prior art documents within this field are as follows:

[0012] International patent application W02023086502 A9 describes compositions comprising an amount of algal protein hydrolysate, an amount of plant protein hydrolysate, an amount of fungal protein hydrolysate, an amount of insect protein hydrolysate, an amount of protein hydrolysate from a multicellular marine organism, or any combination thereof, wherein the composition is optionally a cell culture medium supplement (e.g., a whey substitute), a partially complete cell culture medium, or a complete cell culture medium. The insect hydrolysate is specifically mentioned as being derived from the Oriental cockroach, Blatta orientalis.

[0013] The main difference between the present invention and the previous document lies in the fact that the nutraceutical food product that is intended to be protected has as its main ingredient the insect Blatta orientalis, and it is not related to the chemical process of hydrolysis.

[0014] Another patent-type document is Chinese application CN113631047 A, which relates to a stable protein solution comprising (i) a protein; (ii) a stabilizer; and (iii) a protein deamidase, wherein the solution has a pH of approximately 3.5 to approximately 7.0 and is stable and resistant to protein precipitation. In some embodiments, the solution comprises (i) approximately 0.1% to approximately 30% w / v of protein based on the solution volume; (ii) approximately 0.001% to approximately 5% w / v of stabilizer based on the solution volume; and (iii) approximately 0.5 U to approximately 50 U of protein deamidase activity, or approximately 0.1% to approximately 10% w / w of protein deamidase based on the weight of the protein in the solution.In some embodiments, the solution comprises (i) approximately 0.1% to approximately 30% w / v of protein depending on the volume of the solution; (ii) approximately 0.001% to approximately 1% based on the volume of the solution, weight / volume of stabilizer; and (iii) approximately 5 U to approximately 50 U of protein deamidase activity, or approximately 1% to approximately 10% w / v of protein deamidase activity based on the weight of the protein in the solution.

[0015] Taking into consideration the lessons of Chinese application CN113631047 A, it is important to indicate that although this document refers to a stable mixture of proteins, the present invention is totally different because it obtains an anabolic nutraceutical based on obtaining a tincture from a single insect, Blatta orientala s, whose dynamized dilutions are used to impregnate the vehicle and are applied directly to the food in animal feed.

[0016] OBJECT OF THE INVENTION

[0017] As can be observed, none of the prior art documents manage to establish the objective of the present invention, which refers to the design of an anabolic nutraceutical that in animals for human consumption acts effectively, surpassing the results of others already known in weight gain, mainly lean meat; better health, and better economy.

[0018] A specific objective of this invention is to offer the livestock industry an anabolic nutraceutical product in powder form with a formula different from those currently available on the market. We designed this formula for the finishing stage of beef cattle, seeking, among other anabolic benefits: high efficacy, practicality, safety, cost-effectiveness, and innovation. While it is true that the formula of this application is less complicated and more practical to manage in housed animals, as well as in feeders protected from the elements or in open fields, where the formula is administered by sprinkling it onto the pasture after it has been grazed to become part of the diet, another need is to provide feeders even in free-range grazing areas.

[0019] BRIEF DESCRIPTION OF THE FIGURES

[0020] Figure 1 shows the bar graphs of the control group and experimental group (control blue bars and experimental green bars), reflecting the average gain in Kg / day per animal over a period of 91 days, the experimental group being the 6X anabolic nutraceutical of the present invention.

[0021] Figure 2 shows the graph of weight gain in Kg / day of control groups (blue bars) vs. experimental groups (green bars) during three continuous periods totaling 91 days with the 6X anabolic of the present invention.

[0022] Figure 3a shows the behavior curve of the difference in weight gain of the control group vs. the experimental group, in the fattening period from August 20, 2010 to November 19, 2010, from the beginning to the end of the test.

[0023] Figure 3b shows the average total weight in kg per bovine between the experimental and control groups. It is important to note that the first weighing was at 25 days, the second at 38 days, and the final weighing 28 days after the second, for a total of 91 days. Figure 4 shows a graph of the average daily weight gain in kg for pigs during the 19-day fattening period, in which a 5.4% extra weight gain was achieved.

[0024] Figure 5 shows a graph of feed consumption kg / day in the fattening period of 19 days, where feed consumption savings of between 3.6% and 5.2% are achieved.

[0025] Figure 6 shows the average feed conversion graph, in the fattening period of 19 days, in which with the anabolic 9X, savings in feed conversion were between 8.4% and 12.7%.

[0026] DETAILED DESCRIPTION OF THE INVENTION

[0027] According to the background section, it is possible to indicate that the current state of additives used in animal diets, taking into account that a food additive is defined as a product or substance that is included in the formulation of the diet at a low inclusion level, increasing the nutritional quality of the food and the vitality of the animal; it is not used for its nutritional value since it is assimilated in small quantities, having a high cost (Ravindran, 2010; Rebollar et al., 2015).

[0028] It is important to note that Regulation (EC) No. 1831 / 2003 evaluates and authorizes the marketing and use of feed additives, guaranteeing protection of human health, animal health, and welfare. These additives must favorably influence the characteristics of the feed and animal product in Mexico, have no negative environmental impacts, meet the animal's nutritional requirements, not impair the rumen microbiota, and have a coccidiostatic or histomonostatic effect (Carro et al., 2006). In Mexico, the Federal Commission for Protection against Sanitary Risks (COFEPRIS) is responsible for the regulations governing the use of feed additives (Wilson and Bahna, 2005).

[0029] Furthermore, it is extremely important to handle the ingredients properly and respect the withdrawal times and doses used in the animal diet, in order to obtain a safe and wholesome product for the consumer (Dominguez et al., 2009).

[0030] Before beginning with the detailed description of the invention, it is important to indicate that additives are classified according to their function as antioxidants, colorants, emulsifiers and flavor stabilizers, solvents, glazing agents, sweeteners, preservatives, thickeners and bulking agents (Velázquez et al., 2019).Furthermore, the regulation (EC) categorizes additives into: a) technological, which is any substance added to feed, such as preservatives, antioxidants, emulsifiers, stabilizers, thickeners, gelling agents; b) organoleptic, which is a substance added to feed to improve its properties, whether visual, aroma and consistency; c) nutritional, such as vitamins, chemical substances with analogous effects, amino acids, salts and urea; d) zootechnical, which are used to obtain a positive effect on animal productivity, which can be stabilizers of the intestinal flora; e) coccidiostatic and histomonostatic (Carro et al., 2006).

[0031] In this sense, a growth promoter is a product that increases an animal's weight gain. Not all growth promoters have the same effect, as this depends on their characteristics, chemical structure, and function at the cellular level. Some authors claim that they improve productivity, product quality, reduce the release of nitrogenous waste into the environment, improve feed efficiency in livestock, and decrease pressure on fragile ecosystems (Sillence, 2004; Strydom et al., 2009). The use of anabolic agents with non-hormonal activity is one of the non-genetic methods for modifying the growth potential of animals.

[0032] An anabolic spheroid is defined as any compound or mixture of compounds that affects the metabolic function of the animal to increase the amount of body protein.

[0033] USE OF BETA-ADRENERGIC AGONISTS

[0034] The use of p-adrenergic agonists (pAAs) has been underway for over 50 years. Although some compounds cause health risks, most have minimal impact on animal welfare and consumer health. Their use in sheep has been accepted and researched, although inconsistencies still exist regarding dosage and withdrawal periods (Mersmann, 2002; Sillence et al., 2004).

[0035] The inventors of Clenbuterol sought a medication for asthmatics and those with hypertension; with it, the livestock industry discovered its application in the efficient fattening of animals, which became the most popular anabolic steroid. Due to the negative consequences of Clenbuterol on consumer health, Mexico issued standard NOM-061-ZOO-1999, published in the Official Gazette of the Federation on October 11, 2000, which prohibits its use. Guillén (2022) mentions, among other symptoms, stomach pain, vomiting, tachycardia, and diarrhea; see Marco Antonio Torres-Castro and René Alejandro Torres-Castro, "Bioagrociencias," vol. 16, no. 1. Clenbuterol was banned for the purpose of fattening animals for human consumption due to the negative effects of the agent's accumulation in the liver, muscles, eyes, and hair, and because it causes tachycardia and asphyxiation, which also led to its prohibition in other countries.The inclusion of clenbuterol can cause effects such as increased meat toughness, and this effect is considered particularly unfavorable for meat marketing (Avendaño et al., 2006; Hilton et al., 2009). Naturally, steroid hormones are produced by endocrine glands. Cortisone, aldosterone, androgens, and estrogens, for example, are steroid hormones. Cholesterol and vitamin D are also steroid hormones.

[0036] Senate Gazette. Thursday, March 25, 2004 / GAZETTE: LIX / 1SPO- 40 / 857; letter from the Congress of the State of Jalisco, with which it forwards an agreement to request that controls be established and the serious health problem represented by the use of beta-agonists, especially clenbuterol and zilpaterol, be eradicated.

[0037] REFERRED TO THE AGRICULTURE AND LIVESTOCK COMMITTEE. Source: https: / / www.senado.gob.mx / 64 / gaceta_del_senado / documento / 857A.

[0038] Clenbuterol is registered with the Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA) for use in animals, but only to treat respiratory problems in horses. Therefore, its use as an additive in animal feed constitutes illicit use. (Lie. Humberto Aguilar Coronado, Undersecretary of Legislative Liaison, Secretariat of the Interior, Mexico, May 25, 2004)

[0039] For their part, Kuri et al. (2007) observed that cases of poisoning at the national level increased considerably with a record of more than 133 cases, where alterations in the functionality of the hypothalamic-pituitary-adrenal-gonadal-hepatic axis can be observed, so the need to discontinue its use, until leading to its prohibition by Mexican laws, under federal health regulations since 2002 (Caicedo et al., 2011).

[0040] On June 12, 2022, a food poisoning outbreak was reported affecting 500 people in the municipality of Seyé. The Yucatán Health Secretariat reported that samples taken from affected individuals who presented similar symptoms of probable food poisoning tested positive for Clenbuterol (Hau 2022).

[0041] There are other, safer alternatives to Clenbuterol, such as Zilpaterol or Ractopamine. Zilpaterol was already authorized in Mexico (Official Mexican Standard NOM-061-1999; NOM-EM-015-ZOO-2002), the United States, and South Africa in 2002, where it is marketed as Zilmax. It does not produce adverse health effects in animals or the end consumer (Castellanos et al., 2006). However, according to various sources, this anabolic method is already prohibited in other countries. As of 2013, Zilmax was banned in China, Taiwan, Russia, and many countries of the European Union. On August 6, 2013, Tyson Foods prohibited the import of cattle fed with Zilmax (Merck Animal Health, Merck & Co. Inc.). in their processing plants after hoofless cattle began arriving in large numbers during the warm weather ("BEEFPOINT") 12.08.2013.

[0042] BETA ADRENERGIC AGONISTS

[0043] Beta-adrenergic agonists (pAAs) are a group of drugs (chemical agents) that are applied in animal production to improve nitrogen retention (Dominguez et al., 2009) and belong to the group of elements known as growth promoters. They have hormonal activity with the purpose of increasing muscle growth, feed conversion and feed efficiency, reducing subcutaneous and abdominal fat, thus decreasing costs and achieving superior meat quality (Sillence, 2004; Johnson and Chung, 2007).

[0044] pAAs cause changes in the muscle-to-fat ratio, which occur in the channel (Robles, 2009). They act particularly by stimulating cellular adrenergic receptors, which are located in the cell membranes of target tissues, triggering optimal nutrient uptake and efficient energy and lipolysis, leading to protein synthesis and the formation of muscle mass (Dominguez et al., 2009). (Autonomous University of Baja California, Master's Thesis Lucia De Guadalupe Escobedo Gallegos, Mexicali, Baja California, Mexico, September 2022.)

[0045] 17-13 Estradiol. Anabolic implants are small pellets or rubber pieces made of silicone and / or plastic containing natural hormones (Estradiol, Progesterone, or Testosterone) and / or synthetic hormones (Zeranol, Trenbolone Acetate). They are used to improve weight gain and feed conversion in feedlot cattle. The estrogenic agents they contain increase daily weight gain in cattle by 5% to 15% and improve feed conversion by 5% to 10%. Most contain 17-13 Estradiol, which raises plasma concentrations of insulin-like growth factor and growth hormone (somatotropin), generating a positive nitrogen balance that favors amino acid uptake by cells, increasing protein synthesis and deposition for muscle production.Trenbolone acetate has an additive effect with estrogenic agents, improving daily weight gain by 3% to 5% and feed conversion ratio by 2% to 3%. It increases circulating levels of insulin-like growth factor, promoting nitrogen retention and protein synthesis, as well as the formation and development of skeletal muscle fibers through the activation of the enzyme phosphatidylinositol 3-kinase. Trenbolone acetate has an affinity for the receptor that activates muscle growth and decreases fat tissue by acting on the ribonucleic acid of these cells, increasing lipolysis. It also has the ability to bind to muscle receptors that utilize glucocorticoids, and by occupying these receptors, it inhibits catabolism (muscle breakdown).It improves mineral absorption, promotes red blood cell production and glycogen replenishment, thus aiding the recovery of weak or sick animals. Anabolic implants containing 17-13 Estradiol and Trenbolone Acetate improve growth rate by an average of 10-20%, feed efficiency by 5-15%, and carcass leanness by 5-8%. The Food and Drug Administration (FDA) has determined that it is not necessary to set a maximum residue limit (MRL) for 17-13 Estradiol and Trenbolone Acetate because the amount administered through implants is very low compared to that produced naturally by humans. Therefore, it is unlikely that residues in products from cattle treated with these products pose a risk to human health. Furthermore, it has been proven that 17-13 Estradiol and Trenbolone Acetate are not carcinogenic, genotoxic, or mutagenic.When a production-promoting implant is placed, it gradually releases its content of 17—13 Estradiol and Trenbolone Acetate into the bloodstream, providing a growth-stimulating effect for a long period (Source: https: / / www.ganaderia.com / detacted / Que-son,-como-funcionan-y-que-detalles-debes-cuidar-cuando-utilizas-implantes-anabólicos-esteridos-en-bovinos-en-engorda).

[0046] Anabolic implants with 17-13 Estradiol and Trenbolone Acetate can be combined with our 6X Nutraceutical Anabolic.

[0047] The third beta-agonist is Ractopamine, which is registered with SAGARPA for use as a feed efficiency enhancer in animals, specifically pigs. The following active ingredients are considered beta-agonists, but are not exhaustive: Bromobuterol, Carbuterol, Cimaterol, Cimbuterol, Clenbuterol, Fenoterol, Isoproterenol, Mabuterol, Mapenterol, Orciprenaline, Pirbuterol, Ractopamine, Salbutamol, Terbutaline, Zilpaterol (Source: http: / / sil.gobernacion.gob.mx / Archivos / Documentos / 2004 / 06 / asun_l097863_20040609_1106505.pdf)

[0048] Cockroaches, as part of the diet, constitute an unconventional source of animal protein that is entirely underutilized and could provide significant indirect nutritional value. The protein content was determined to be 58%, and the essential amino acid profile of this protein is very similar to the amino acid profile of the protein recommended by the FAO in 1973. It was found that cockroach meal can replace up to 20% of soybean meal in a chicken diet. Based on the statistical analysis of the results of this chick feeding experiment, it can be suggested that up to 20% of the soybean meal in a chicken diet can be replaced by cockroach meal, without showing differences in weight gain, feed intake, or feed conversion ratio. (Lacandonia, Rev. Ciencias UNICACH 3 (1): 59-68. (2009)).

[0049] Blatta orientalis (Indian Domestic Cockroach)

[0050] Its field of action is limited to asthma, an effect discovered by chance by a very observant patient who drank tea from a teapot in which a cockroach had been boiled. He obtained great relief from his asthma after drinking the infusion, in which he later found the cockroach. It is one of the most interesting remedies for this problem, and Anschutz advises administering, in acute episodes, potencies of the order of 1. a or 2 a X crush every 15 minutes to two hours, and discontinue when the seizure subsides. In chronic treatment, give it at 3 aX, 2 to 4 times a day, 15 to 30 days; in many cases it acted almost specifically; 1 - (+++) Asthma, especially in stout people, particularly associated with bronchitis. Severe attacks of cough with dyspnea. The dyspnea is worse at night and when lying down and better with expectoration. Attacks worse in rainy weather. Imminent suffocation due to a large accumulation of mucus. Tuberculosis with much mucopurulent expectoration; 2 It has cured cases of anasarca, after Apis Apocynum and Digitalis failed (Source: "Homeopathic Materia Medica of Vijnovsky Argentina 1981).

[0051] Unlike the foregoing, the present invention proposes a lactose-based nutraceutical additive with 1 mm diameter sugar microglobules to induce anabolism in fattening cattle. Its main active ingredient is Blatta orientalis 6X (among other dilutions / potentizations), obtained from the mother tincture according to Hahnemannian Rule 4 of preparation. For the invention of our formula, we decided to experiment with this insect, a cockroach of the Blattidae family native to Europe, due to its extraordinary resistance to surviving even with reduced oxygenation. Thus, we were able to establish as our main objective the implementation of the proposed nutraceutical anabolic agent function using the principles and foundations of the homeopathic system, improving tissue oxygenation in fattening cattle.And thus, to avoid adverse health effects on consumers (and animals) of this meat, the use of our anabolic agent is proposed.

[0052] Due to the methodology under which we designed the composition of the anabolic agent of the present invention using this insect, we achieved very favorable weight gain results in cattle at very accessible investment costs for livestock farmers, and through the dilution scale, we aimed to achieve zero toxicity. To date, we have not found anything similar in efficacy for this purpose of weight gain in fattening cattle—primarily beef and swine—and on lean meat.

[0053] PRODUCT OBTAINING

[0054] PREPARATION OF THE ANABOLIC OF THE PRESENT INVENTION

[0055] The invention relates to a purely natural nutraceutical made from an insect (Blatta orientalis), which is the source of the active ingredients. The insect is crushed in 87° cane alcohol, which is added progressively until the alcohol used reaches five times the weight of the insect. The mixture is placed in a new, sterile, amber glass bottle, sealed with an inert plastic or cork stopper, ensuring that the bottle is filled to a maximum of 75% capacity to allow for movement of the contents. The bottle is tapped 100 times, twice a day, for a period of 14 continuous days to obtain a product classified as a base or tincture. This tincture is then progressively diluted / potentized to various degrees, including ultra-dilution / potentization. We can choose between decimal or centesimal dilutions (for further understanding, see APPENDIX 1).

[0056] Decimal scale dilutions consist of one part of the previous dilution plus nine parts of 87° cane alcohol; furthermore, by inducing at least one hundred percussions, we obtain dilution IX, and so on from IX to 10X or more. To progressively increase the degree of a new dilution, the dilution immediately preceding the new scale is always used.

[0057] In the case of choosing the centesimal dilution, one begins with one part of the tincture plus 99 parts of 87° cane alcohol, subjecting the flask of this new combination to at least 100 percussions. This yields the 1cH scale; and so on, progressing from 1cH to 30cH or higher. Similarly, always escalating progressively with the immediately preceding dilution to the new degree of dilution, in addition to the inherent and indispensable induction of 100 percussions prior to each step up in dilution. This process continues until the chosen scale is established. To achieve the proposed and experimental anabolism in cattle, the most frequently used dilution by our team was 6X; for each dilution scale, a single new amber glass flask is required.

[0058] Once the desired concentration in liquid (87° alcohol) is achieved, it is used to impregnate (in the case of the present invention for anabolic purposes) the vehicle, which consists of a mixture composed of 60% lactose powder and 40% cane sugar microglobules, 1 millimeter in diameter. Sufficient impregnation is achieved by spraying—10 ml of this anabolic formula per 1 kg of vehicle—without clumping or forming lumps, so that it remains a slightly moist powder with a sufficient alcohol aroma.

[0059] Through the potentiation of the active principles contained in Blatta orientala, as the main source of active substance, it is converted into a dynamized preparation through percussion and diluted between each scale. This promotes its anabolic nutraceutical action. The concentration doses of the active principles contained in this insect are minuscule amounts—for example, in 6X for cattle the average is approximately 13 nanograms, and in 9X dilution the concentration dose is approximately 13 picograms of active substance per bovine per day of dosing—achieving a highly efficient effect; furthermore, it combines the advantages of several anabolic agents available on the market.

[0060] It should be noted that the above dilutions have been used not only in cattle, but also in other animal species such as sheep, the Blatta orientalis dilution is more effective, comparing the dynamizations 6X, 9X, 6c, 30c, vs. control, so even though these are not mentioned in the examples due to lack of data, it can be observed that the 9X dynamization gave better results in weight gain in pigs (example 2).

[0061] INSTRUCTIONS FOR USE

[0062] The anabolic is sprinkled onto the freshly served pasture in the feeder before the animals begin to taste it.

[0063] Regardless of the age or weight of the bovine (in other animal species the proportions of formula and the amount per animal per day of dosage are adjusted) generally the recommended dose is 1 gram per head once a day, for three continuous days and without dosing for seven continuous days, thus completing cycles of 10 days until the weight gain required by the market is reached.

[0064] The formula, dosage, administration, system, and preparation method for this product allow for enhanced anabolic performance in cattle during the finishing phase prior to slaughter for consumption. This phase typically lasts from 90 to 120 days, and sometimes up to 150 days, depending on the farmer's objectives, as well as factors such as diet, climate, and breed characteristics. In species other than cattle—such as swine, sheep, and / or chickens—the fattening period is necessarily shorter. Using the same active ingredients, this formula is suitable for smaller species according to their specific needs.

[0065] APPLICATION EXAMPLES

[0066] Once the detailed description of the invention has been indicated, we will specify the following examples, which are not limiting.

[0067] Test on cattle (example 1)

[0068] RESULTS

[0069] Table 1 below shows results obtained in cattle from the control group, exempt from the 6X anabolic nutraceutical formula of the present invention, in pen 12. These results are compared to the results in Table 2 from pen 13, which is the experimental group for our 6X anabolic nutraceutical. Table 1

[0070] Table 2 below shows results obtained in cattle with the magistral formula of Anabolic nutraceutical 6X, of the present invention, in pen 13.

[0071] Table 2 As can be seen in Tables 1 and 2, the most productive period for weight gain (kg / day) during the fattening phase was the first period, which lasted 25 days. With the Anabolic 6X supplement from this application versus the control group, the average difference in weight gain for the experimental group in each period was: 1st period 0.228 kg / day; 2nd period 0.579 kg / day; 3rd period 0.501 kg / day. Throughout the entire 91-day period, the Anabolic 6X supplement maintained an average additional weight gain of 0.459 kg / day.

[0072] Figure 2 shows the average daily weight gain in kg per animal over a 91-day period. It should be noted that the experimental group refers to the 6X anabolic group, which is the anabolic agent of the present invention. It is important to observe that the bars for the experimental group are consistently more productive with the 6X anabolic formula, especially in the first period. This equates to 2.543 kg / day of weight gain, representing a 9.85% increase compared to the control group.

[0073] Figure 2 shows the control group versus the experimental group, reflecting the utilization of the most productive period (weight gain kg / day) with the formula of our 6X Anabolic of this invention. The last two bars represent the overall average for the 91-day period. Also shown are the averages typically achieved by others with clenbuterol, and also without any anabolic additive.

[0074] Figure 3a shows the graph illustrating the average difference in kilograms between the control group (weighing less at the start of the test, 6.9 kg) and the experimental group with the 6X anabolic nutraceutical of the present invention. It is important to note that the test ended with an average weight difference of 48.7 kg greater than the control group.

[0075] Figure 3b shows the average weight difference per bovine in kg between the control group and the experimental group. The trial period lasted 91 days, and the experimental group (6X anabolic nutraceutical) showed a significant advantage, with a weight gain of 48.7 kg compared to the control group. Based on this experiment, it is reasonable to highlight the following advantages offered by this invention, specifically the anabolic nutraceutical formulation:

[0076] The amount of active substance from Blatta orientalis—the source of the active ingredients—induced in a bovine at a 6X scale is minimal, at only about 13 nanograms per dose. At a 9X scale, it is approximately 13 picograms per dose of the active ingredient source.

[0077] - It allows you to maintain regular feed diets.

[0078] - Easy to apply.

[0079] - It helps maintain the palatable flavor of the meat.

[0080] - The protein synthesis it induces mainly produces lean meat.

[0081] - Improves the animal's ventilation and maintains its overall health in better condition.

[0082] - Less passive animal behavior.

[0083] - Zero toxicity for the animal, and equally for the consumer of this meat.

[0084] Test on pigs (example 2)

[0085] In another test, the effect of the present invention's anabolic nutraceutical product at a 9X dilution scale was determined on weight gain and feed conversion when used as a feed nutraceutical during the fattening stage of swine. In this example, the 9X Blatta dilution scale was chosen, which, for swine, contains a metrically measured active substance concentration of approximately 4 picograms per swine per day of dosing.

[0086] Research sites

[0087] Animal phase

[0088] Location: "Timo's Farm"

[0089] Type of operation: semi-technified

[0090] Location: 20°33'27.4"N 103°21'06.7"W Climate: min 19°C and max 34°C

[0091] Test execution

[0092] Materials and methods

[0093] Sixteen York / Pietran / Landrace breed pigs were used, within the weight range of 55 to 85 Kg and 5 months of age, healthy and born within the same facilities.

[0094] Start of testing

[0095] The application of our 9X Anabolic Nutraceutical product begins on May 13, 2024; concluding on May 29, 2024.

[0096] Pure experimental design

[0097] Pure: Where the effect of the independent variable on a dependent variable will be divided into two groups.

[0098] Experimental: supplementation of 9X anabolic nutraceutical in food.

[0099] Control: Food without supplement

[0100] Inclusion criteria

[0101] 1. Species: Swine

[0102] 2. Race: Not specified

[0103] 3. Age: 5 months

[0104] 4. Weight: between 65 and 85 kilos

[0105] 5. Sex: not specified

[0106] 6. Physiological state: healthy animals that meet physiological constants within normal ranges.

[0107] Exclusion criteria

[0108] Age: over 5 months

[0109] Weight: outside the range of 55 to 85 kilos

[0110] With a history of applying the 9X anabolic nutraceutical product.

[0111] Animals in stressful situations.

[0112] Physiological state: animals that, during the physical examination, do not meet normal ranges. Animal handling

[0113] The animals used in this study were born under the conditions of the research site, previously dewormed with Invermex 1% (ivermectin), and given 10% iron supplementation on the third day of life prior to the experiment. They were also supplemented with the product TROPTIMS for 4 months, which stimulates growth.

[0114] ID

[0115] The animals were identified by tattooing numbers consecutively to the animals at random, the groups were arranged sequentially and divided into the groups in Table 3:

[0116] Table 3

[0117] Facilities

[0118] The piglets were housed in pens measuring 2.40 x 3.00 meters.

[0119] Feeding

[0120] The pigs' feed is based on 30% soy and 70% cold tortilla (approximately 48 hours old), supplemented with a mineral premix with vitamins; and for control pigs, with medicated additives containing Ractopamine hydrochloride, where each 1000 g contains at least 14.5% and phosphorus 0.4% for finishing pigs. Water: Ad libitum

[0121] General clinical examination

[0122] An examination was performed to verify that the animals are within the normal physiological constants of the species, as well as the weighing of the animals using a high precision digital hanging scale with a capacity of 100kg, Mini Crane brand, model WH-7C100.

[0123] Temperature: 38 - 40.5 °C

[0124] Heart rate: 60-90 BPM

[0125] Respiratory rate: 10-20 RPM

[0126] Capillary fill time: 2.0 to 3s

[0127] Treatment :

[0128] Product information: Anabolic 9X nutraceutical.

[0129] Presentation: 50g sachet

[0130] Lot: Unique 10 / 05 / 2024

[0131] Date of preparation: 10 / 05 / 2024

[0132] Expiration date: 09 / 05 / 2025

[0133] Formulation: Oriental larvae 9X; Lactose 60%; sugar microglobules 40%:

[0134] Application type

[0135] The application is oral through food.

[0136] Application schedule, shown in Table 4

[0137] Table 4

[0138] The Anabolic 9X will be administered orally with the first feeding of the day, sprinkled onto the cattle's regular feed. Each feeding will be weighed before being placed in the manger, followed by adding Anabolic 9X (0.33 g per head per day for pigs) to the pasture. Once all the animals have finished eating, the remaining feed will be weighed again to estimate feed and medication consumption. The initial and final weights, as well as the difference in feed intake, will be recorded daily in a logbook.

[0139] Dosage and route of application

[0140] Two groups will be formed as follows:

[0141] Group 1:

[0142] Treatment with the 9X Anabolic nutraceutical product at a dose of 0.3 g per dose, once a day for 3 consecutive days, followed by an 11-day break, for 2-week cycles.

[0143] Group 2:

[0144] Negative control, only food is administered without any kind of additive other than the one mentioned above.

[0145] Adverse effects

[0146] Not detected

[0147] Results shown in Table 5

[0148] Table 5 Table 5 shows the age and sex of the pigs, and their performance at the beginning and end of the fattening stage. Group L1, treated with the anabolic nutraceutical 9X, is the experimental group; group L2 is the control group, which received Ractopamine. In all aspects, the average difference in the results is significantly favorable: the extra weight gained per animal in 19 days was 1.019 kg higher in group L1, that is, 0.054 kg / day; feed consumption was higher in group L2 by 0.143 kg / day per animal; and a reduction in feed conversion was also observed in group L1.

[0149] 10.5% (±2.2) .

[0150] Figure 4 shows a graph of the average weight gain in Kg / day in pigs during the 19-day fattening period, in which a 5.4% extra weight gain is achieved, and this is a significant percentage when comparing this test group of the 9X Anabolic vs. the control group using ractopamine.

[0151] Figure 5 shows a graph of feed consumption (kg / day) during the 19-day fattening period, where feed consumption savings of between 3.6% and 5.2% were achieved. This is a seemingly low percentage during this fattening period, and it is significant because the test group using the anabolic steroid 9X was compared to the control group using ractopamine.

[0152] Figure 6 shows the average feed conversion ratio graph for the 19-day fattening period, in which the anabolic agent 9X resulted in feed conversion savings of between 8.4% and 12.7% during that period. This means that with only approximately 89% (±2) of the feed, the same performance was achieved compared to the control group that included ractopamine.

[0153] Tolerance to oral administration:

[0154] No intolerance or rejection reactions to the food were observed following oral administration. Nor did any animal experience regurgitation or diarrhea during the trial.

[0155] Adverse effects attributable to the product:

[0156] The Anabolic 9X nutraceutical product demonstrated a wide range of safety for use, as it did not present any adverse events after oral administration.

[0157] In view of what has been set forth in this descriptive chapter, it is reiterated that the scope of the present invention shall not be limited by the specific embodiments described, and it shall be understood that variations may be made in several respects. Such variations shall not be considered a departure from the spirit and scope of the invention, and all such modifications may be obvious to a person skilled in the art and shall be included within the scope of the following claims.

[0158] Annex 1

[0159] For a better understanding of the invention, the method of homeopathy used is indicated in this Annex 1, as well as explaining what is related to the magistral formula.

[0160] The homeopathic method involves dilution and potentization. For the preparation of this homeopathic dilution with the insect Blatta orientalis, the Rules of Preparation of the Homeopathic Pharmacy were taken into account, and from these, rule 4 was used, which was partially modified by extending the period from 8 days to 14 days of duration; the number of percussions was also doubled to 100, inducing 200 blows twice a day for this preparation.

[0161] In another part of the method for obtaining homeopathic products, and for this case, it is necessary that in a porcelain mortar -preferably new to ensure the purity of the result- you add in fractions the corresponding pure 87° cane alcohol when the Blatta orientalis insect is crushed.The proportions of the ingredients for this grinding are, for example: if four or five specimens of this insect weigh 4 grams, progressively add 20 grams of 87° pure cane alcohol, which corresponds to approximately 25 ml. This is five times the insect's mass. After this grinding (which takes about 40 to 60 minutes), a homogeneous consistency is obtained. Then, 18 grams of this paste—approximately 22 ml—are placed into a new 30 ml amber glass jar, sealed tightly, and the jar is tapped 200 times on a slightly cushioned surface—such as a leather-bound book—twice daily for two weeks. At the end of this process, we achieve the concentration known as a tincture.

[0162] To continue with the dilution process in this case on a decimal scale; in another new 30 ml amber glass bottle we introduce 2 ml of "Blatta orientalis tincture", we add 18 ml of 87° pure cane alcohol; we seal it tightly; and we induce the bottle to percussion by making 200 blows on a slightly cushioned surface - such as a leather-bound book -; with this we obtain the Blatta orientalis IX dilution.

[0163] Continuing this process to create a new, gradually progressive dilution, in another new 30 ml amber glass bottle, we introduce 2 ml of "Blatta orientalis IX," add 18 ml of 87° pure cane alcohol, seal tightly, and tap the bottle 200 times on a slightly cushioned surface—such as a leather-bound book—to obtain the Blatta orientalis 2X dilution. We continue this gradual and progressive process for each new dilution. For each subsequent dilution, it is necessary to always introduce 2 ml of the previous dilution into a new bottle, add the corresponding 18 ml of 87° pure cane alcohol, seal tightly, and tap the bottle 200 times on a slightly cushioned surface—such as a leather-bound book. And here at this point the new scale is achieved.And so on until the desired scale is reached, for example: 6X, 9X, 12X, or more; the one chosen according to the purpose.

[0164] Gradually progressive dilution, with its corresponding dynamization by inducing percussions on the bottle through at least 100 blows at each new dilution scale, generally turns the most dangerous substances in their natural state into some of the best homeopathic medicines.

[0165] Therefore, this characteristic of Blatta orientalis, the resistance of some to live without air for up to 45 minutes, is transformed through this homeopathic system into its opposite action, and with this we can induce an increase in oxygenation in the tissues of animals. This generalized increase in oxygenation throughout the organism also accompanies an increase in blood supply to the muscle fibers, increases their nutrition, increases their size, and increases the number of muscle cells; favoring greater muscle mass—something similar to when doing physical exercise.

[0166] Here I will mention a few of the principles of the homeopathic system, which in part justify the design of the formula of this nutraceutical anabolic invention:

[0167] Rule 4 (2nd edition of Materia Medica Pura) Tinctures prepared with five parts by weight of concentrated alcohol... and the live animals very well crushed, shall be weighed and placed in five parts of concentrated alcohol in a well-sealed flask for 8 days. It shall be kept at a moderate temperature in a dark place, shaken twice a day. After this time, it shall be decanted, squeezed, and filtered. Source: https: / / docplayer.es / 27374506-Farmacia-homeopatica-reglas-de-preparacion.html

[0168] Paragraph 269. The homeopathic method, by its own procedure, develops the dynamic medicinal virtues of crude substances, giving them all a profoundly effective and therapeutic action, even those which in their raw state showed no sign of the slightest medicinal influence on the human body. This remarkable change in the qualities of natural substances develops the latent dynamic power... as if it had remained hidden or dormant, a power that influences the vital principle and modifies the mode of being of animal life. This is accomplished by mechanical action on their smallest particles by rubbing and shaking... This process is called dynamization, "potentization" (development of medicinal power), and from it result dynamizations or potencies in different degrees. Source: Organon of Medicine "Dr. Samuel Hahnemann" translation of the 6 a edition.

[0169] Footnote No. 200.

[0170] ...whereas there exists a law of nature according to which physiological and pathogenic changes occur in the body by means of forces capable of altering the raw material state of drugs, even those that have never before exhibited any medicinal virtue. This is accomplished by trituration and succussion... Source: Organon of Medicine "Dr. Samuel Hahnemann" translation of the 6 a edition.

Claims

CLAIMS 1.- An anabolic nutraceutical formulation characterized in that it comprises a diluted / dynamized tincture of Blatta oriéntalas; lactose 60%; sugar microglobules 40%. 2.- The anabolic nutraceutical formulation of claim 1, characterized in that the diluted / dynamized tincture of Blatta orientalis can be 6X or 9X. 3.- The anabolic nutraceutical formulation of claim 2, characterized in that the diluted / dynamized tincture of Blatta orientalis 6X is equivalent to 13 nanograms of Blatta orientalis per dose per animal head. 4.- The anabolic nutraceutical formulation of claim 2, characterized in that the diluted / dynamized tincture of Blatta orientalis 9X is equivalent to each dose per animal head in a range of 4 to 13 picograms of Blatta orientalis. 5.- The anabolic nutraceutical formulation of claims 3 and 4, characterized in that the animals can be bovine, porcine or ovine. 6.- A procedure for preparing the anabolic nutraceutical formulation characterized in that it comprises the steps of: a) crushing the insect Blatta orientalis, in 87° cane alcohol, which is added progressively until the alcohol reaches five times more than the weight of the insect; b) depositing the mixture from step a into a sterile amber glass bottle, sealed with a lid; c) striking the bottle with 100 blows, twice a day, for a period of 14 continuous days until a tincture is obtained; d) perform a dilution / dynamization, which is obtained with 9 parts of 87° cane alcohol if the dilution is decimal and 99 parts of 87° cane alcohol if the dilution is centesimal; e) tap the dilution / dynamization of step d) 100 times, until a dilution IX is obtained; f) repeat steps d) and e) until 10X or more dilutions are obtained; g) impregnate the dilution in the vehicle which consists of a mixture of 60% lactose powder and 40% cane sugar microglobules of 1 mm in diameter. 7.- The procedure for preparing the anabolic nutraceutical formulation, according to claim 6, characterized in that in step g) the impregnation of the mixture is by atomization or spraying. 8.- The procedure for preparing the anabolic nutraceutical formulation, according to claim 7, characterized in that the impregnation of the mixture is 10 ml of the formula per 1 kg of vehicle. 9.- Use of the anabolic nutraceutical formula of claim 1, characterized in that this formula is sprinkled on freshly served animal pasture. 10.- The use of the anabolic nutraceutical formula according to claim 9, characterized in that the recommended dose is 1 gram per head once a day for three continuous days and without dosing for seven days until completing cycles of 10 days and reaching the end of the required weight gain. 11.- The use of the anabolic nutraceutical formula according to claim 9, characterized in that the animals can be selected from among cattle, pigs, sheep and / or chickens.

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