Separation of virus particles with ligating filter elements

Ligating polymers with hydrophobic quaternary ammonium groups enhance the separation of full and empty AAVs by increasing salt tolerance and conductivity windows, addressing inefficiencies in current methods and ensuring safer gene therapy treatments.

WO2026112096A2PCT designated stage Publication Date: 2026-05-28THERMO FISHER BIOPROCESSING INC
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Patent Information

Application Number
PCT/US2025/056033
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-11-19
Filing Date
2025-11-19
Publication Date
2026-05-28

AI Technical Summary

Technical Problem

Current methods for separating full adeno-associated viruses (AAVs) from empty AAVs are inefficient due to their identical shape and size, leading to low salt tolerance and unclear separation windows, which increases the risk of adverse side effects in gene therapy treatments.

Method used

Development of ligating polymers with hydrophobic quaternary ammonium groups that bind to the internal hydrophobic amino acids of AAVs, allowing for higher salt tolerance and a wider conductivity window for separation, enabling clear distinction between full and empty AAVs.

Benefits of technology

The new filter elements provide improved salt tolerance and separation efficiency, reducing the need for additional salt removal steps and ensuring safer gene therapy treatments by minimizing impurities.

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Abstract

This present disclosure is directed to ligating monomers and polymers prepared therefrom are described. Filter elements including the ligating polymers are further described as are the uses thereof for separation of adeno-associated viruses containing a DNA payload ("full AAV") from adeno-associated viruses without a DNA payload ( "empty AAV).
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