Prime editing system-based method for achieving inversion editing of large genomic fragment, and composition used

Abstract

Provided are a prime editing system-based method for achieving inversion editing of a large genomic fragment and a composition used. By means of the efficient multi-gene prime editing protein construct ePPEplus in plants, the inversion of large fragments in plants is achieved by means of designing paired pegRNAs (pegL and pegR), and then by means of processes such as reverse transcription, DNA double-stranded annealing, DNA strand synthesis, and repair. The pegL comprises RTT-L, and the pegR comprises RTT-R. The nucleotide sequence of RTT-L is identical to that of strand A at the right terminus of a double-stranded DNA to be inverted. The nucleotide sequence of RTT-R is identical to that of strand B at the left terminus of said DNA. By means of testing in the monocotyledonous plant wheat and dicotyledonous plants Nicotiana benthamiana and tomato, it is indicated that the method is an effective method for mediating precise inversion of large-fragment DNA in plants.

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