Improved protocol for random mutagenesis
The use of a free radical scavenger in conjunction with mutagens like EMS or ENU in plant cells addresses the issue of seed germination inhibition and mutation frequency compromise, enhancing plant development and yield in mutagenesis protocols.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- KEYGENE NV
- Filing Date
- 2025-11-28
- Publication Date
- 2026-06-04
AI Technical Summary
Existing chemical mutagenesis protocols for plant cells result in high inhibition of seed germination, compromising the germination quality and mutation frequency, necessitating an improved protocol that maintains high mutation frequency while minimizing detrimental effects on plant development.
A method involving exposure of plant cells to a mutagen, such as alkylating agents like EMS or ENU, in conjunction with a free radical scavenger like resveratrol, to reduce mutagen-induced detrimental effects on plant development and enhance germination and mutation frequency.
The method enhances plant survival rates, germination rates, and mutation frequency, resulting in a higher yield of mature and seed-bearing mutagenized plants, while maintaining similar mutation frequencies compared to protocols without scavengers.
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