Methods and composition for producing a heterologous protein

Abstract

Described herein are methods and compositions for producing heterologous proteins. Heterologous proteins can include sweet proteins and sweet polypeptides. For examples, described herein are expression cassettes, including, for example, promoters and signal peptides, for recombinantly expressing a recombinant sweet protein in a host cell.

Description

METHODS AND COMPOSITION FOR PRODUCING A HETEROLOGOUS PROTEINCLAIM OF PRIORITY

[0001] This application claims the benefit of U.S. Provisional Application Serial No. 63 / 725,874, filed on November 27, 2024. The entire contents of the foregoing are incorporated herein by reference.SEQUENCE LISTING

[0002] This application contains a Sequence Listing that has been submitted electronically as an XML file named "54282-0016W01_ST26.xml". The XML file, created on November 24, 2025, is 296,837 bytes in size. The material in the XML file is hereby incorporated by reference in its entirety.TECHNICAL FIELD

[0003] This disclosure relates to methods and compositions for producing heterologous proteins. In particular, provided herein are expression cassettes and components thereof (e.g., promoters and signal peptides) that are useful in the production of heterologous proteins, including the production of recombinant sweet proteins.BACKGROUND

[0004] Recombinant expression provides an alternative production means for proteins, for example, proteins used for food and nutrition. In some cases, recombinant proteins can provide healthy diet alternatives. In one such example, various sweeteners are available, including naturally occurring carbohydrates, such as those obtainable from plants, and artificial sweeteners. However, high levels of sugar consumption have been linked to obesity and associated health problems, including an increased risk of high blood pressure, high cholesterol, Type 2 diabetes, and cardiovascular disease. Yet, sweet food products are favored by a large proportion of the population. Therefore, there is a need to provide and produce adequate amounts of healthy sweetener alternatives that deliver similar or improved qualities to currently available sugar and sugar alternatives, such as by expressing sweet proteins.SUMMARY

[0005] Producing proteins through recombinant expression can provide alternative sources for food, flavorings, and nutrition. However, there remains a challenge to produce proteins in sufficient quantitiesin a cost-efficient manner to make the recombinant protein production economically viable and to provide such proteins in sufficient quantity and quality to be compatible with the existing food supply chain. Such challenges can include both the amount of protein expressed in the selected host organism for recombinant expression, as well as the amount that can be extracted and purified away from unwanted host cell components. There remains a need for tools for recombinant expression, particularly for proteins that appear to be poorly expressed and / or secreted when placed in a heterologous host cell. Accordingly, provided herein are promoters comprising a first nucleotide sequence comprising a first nucleotide sequence set forth in any one of SEQ ID NOs: 3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, or 235-239.

[0006] Also disclosed herein are expression cassettes including a promoter operably linked to a coding sequence of a heterologous protein, wherein the promoter comprises a first nucleotide sequence selected from any one of SEQ ID NOs: 232-238, and wherein the heterologous protein is operably linked to a signal peptide. In some cases, the signal peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 66-71, 79, 81, 82, 85, 97, 100, 106, 107, 109, 113-116, 119, 120, 122, 125, 127, 134, 144, 145, 149, 150, 155, 158, 159, 166, 174, and 203. In some cases, the heterologous protein is selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

[0007] Also disclosed herein are systems including any of the expression cassettes described herein, and a second protein comprising the amino acid sequence of SEQ ID NO: 240 or 241. In some cases, the coding sequence of the second protein is comprised within a second expression cassette and operably linked to a second promoter. In some cases, the second promoter comprises a second nucleotide sequence selected from any one of SEQ ID NOs: 7, 33, and 65.

[0008] Also disclosed herein are eukaryotic cells including any of the expression cassettes described herein or any of the systems described herein. In some cases, the cell a yeast cell or a filamentous fungal cell. In some cases, the cell is a Komagataella sp., optionally Komagataella phaffii.

[0009] Also disclosed herein are expression cassettes including a promoter operably linked to a coding sequence of a heterologous protein, where the promoter includes a first nucleotide sequence selected from any one of SEQ ID NOs: 3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, or 235-239. In some cases, the expression cassette also includes a second nucleotide sequence encoding a signal peptide linked to the coding sequence of the heterologous protein. In some cases, the heterologous protein includes a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

[0010] Also disclosed herein are expression cassettes including a promoter operably linked to a coding sequence of a heterologous protein that is a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, mycodulcein or a functional fragment thereof, wherein the promoter comprises a first nucleotide sequence selected from any one of SEQ ID NOs: 1-34, 65, or 232-238. In some cases, the expression cassette also includes a second nucleotide sequence encoding a signal peptide linked to the coding sequence of the sweet protein. In some cases, the first nucleotide sequence is selected from the group consisting of SEQ ID NOs: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, 65, 232, 233, 234, 235, 236, 237, and 238. In some cases, the second nucleotide sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 66, 81, 82, 97, 144, 145, 149, 150, 155, 159, 166, 174, and 189-200. In some cases, the amino acid sequence is selected from the group consisting of SEQ ID NOs: 81, 82, 97, 144, 155, 190, and 195. In some cases, the heterologous protein comprises brazzein, brazzein 53, brazzein-54 or a functional fragment thereof. In some cases, the second nucleotide sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 100, 106, 107, 109, 113-116, 119, 120, 122, 125, 127, 149, 155, 158, and 203. In some cases, the amino acid sequence is selected from the group consisting of SEQ ID NOs: 100, 109, 115, 119, 120, 149, and 158. In some cases, the heterologous protein comprises monellin or a functional fragment thereof. In some cases, the second nucleotide sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 67-71, 79, 81, 85, 116, 134, and 150. In some cases, the amino acid sequence is selected from the group consisting of SEQ ID NOs: 69, 79, 81, and 85. In some cases, the heterologous protein comprises thaumatin, thaumatin I, thaumatin II or a functional fragment thereof.

[0011] Also disclosed herein are signal peptides including the amino acid sequence set forth in any one of SEQ ID NOs: 99-107, 109-158, or 203-206.

[0012] Also disclosed herein are protein coding sequences including a nucleic acid encoding any of the signal peptides described herein or a signal peptide with an amino acid sequence set forth in any one of SEQ ID NOS: 67, 75, 77, 81, or 88 fused in frame to a coding sequence for a heterologous protein. In some cases, the signal peptide is 5' to the heterologous protein. In some cases, the signal peptide is 3' to the heterologous protein. In some cases, the heterologous protein is a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, mycodulcein or a functional fragment thereof.

[0013] Also disclosed herein are protein coding sequences including a nucleic acid encoding a signal peptide comprising the amino acid sequence set forth in any one of SEQ ID NOS: 66-206 fused in frame toa coding sequence for a heterologous protein that is a sweet protein selected from the group consisting of a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

[0014] Also disclosed herein are expression cassettes including any of the protein coding sequences described herein. In some cases, the expression cassette also includes a promoter, where the promoter comprises a first nucleotide sequence set forth in any one of SEQ ID NOS: 1-34, 65, or 232-238.

[0015] Also disclosed herein are vectors including any of the promoters disclosed herein, any of the expression cassettes disclosed herein, any of the signal peptides disclosed herein, and / or any of the protein coding sequences disclosed herein, optionally operably linked to any of the promoters disclosed herein and / or any of the signal peptides disclosed herein.

[0016] Also disclosed herein are expression vectors including any one of the promoter-signal sequence combinations set forth in Table 11, optionally, wherein the expression vectors include a heterologous protein. In some cases, the heterologous protein is selected from the group consisting of a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

[0017] Also disclosed herein are cells including any of the promoters disclosed herein, any of the expression cassettes disclosed herein, any of the signal peptides disclosed herein, and / or any of the protein coding sequences disclosed herein, optionally operably linked to any of the promoters disclosed herein and / or any of the signal peptides disclosed herein. In some cases, the cell is a eukaryotic cell. In some cases, the cell a yeast cell or a filamentous fungal cell. In some cases, the cell is Komagataella sp., optionally Komagataella phaffii.

[0018] Also disclosed herein are methods of producing a heterologous protein, the method including inserting any of the expression cassettes disclosed herein into an expression system whereby the heterologous protein is produced. In some cases, the disclosed methods further include the co-expression or overexpression of a transcription factor. In some cases, the disclosed methods further include the coexpression or overexpression of a transcription factor whose amino acid sequence is set forth in SEQ ID NO: 240 or SEQ ID NO: 241. In some cases, the expression system is a cell. In some cases, the expression system is a eukaryotic cell. In some cases, the cell a yeast cell or a filamentous fungal cell. In some cases, the cell is Komagataella sp., optionally Komagataella phaffii. In some cases, the heterologous protein is secreted into a culture medium. In some cases, the method also includes collecting the culture medium. In some cases, the method also includes isolating the heterologous protein from the culture medium. Insome cases, the heterologous protein is a sweet protein selected from the group consisting of a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

[0019] The details of one or more embodiments of the invention are set forth in the accompanying description below. Other features, objects, and advantages of the invention will be apparent from the description, and from the claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Methods and materials are described herein for use in the present invention; other, suitable methods and materials known in the art can also be used. The materials, methods, and examples are illustrative only and not intended to be limiting. All publications, patent applications, patents, sequences, database entries, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control.DETAILED DESCRIPTION

[0020] Described herein are promoter sequences that drive gene expression of heterologous proteins. Also described herein are signal peptide sequences for secretion of heterologous proteins and nucleotide sequences encoding such signal peptide sequences. Also described herein are cells, expression cassettes, and vectors that include the promoters, the signal peptides, and combinations thereof, for recombinantly expressing a heterologous protein in a host cell. In some particular cases, the expressed heterologous protein is a sweet protein.I. Compositions for Producing Heterologous Proteins or Heterologous Peptides

[0021] Provided herein are compositions for producing and secreting heterologous proteins, such as sweet proteins. Compositions for producing heterologous proteins (e.g., sweet proteins; also called sweet polypeptides) include expression elements, such as promoters, signal peptides, and terminators. These elements can be incorporated into expression cassettes. Such expression cassettes are described and provided herein. Expression cassettes can be incorporated into vectors (also called expression vectors). Expression cassettes and / or vectors can also be incorporated into host cells to facilitate production of a heterologous peptide (e.g., a sweet polypeptide). Expression systems are also provided for producing the heterologous protein. Such expression systems can be a cell, such as a host cell that includes one or more expression cassettes or vectors. Host cells can include eukaryotic cells, such as yeast cells or filamentousfungal cells. Exemplary yeast cells include cells of Komagataella sp. (also known as Pichia sp.), such as Komagataella phaffii.Engineered Promoters

[0022] A promoter is a nucleic acid sequence that, when operably linked to another nucleic acid sequence such as a nucleic acid sequence encoding a polypeptide (e.g., a recombinant peptide, such as a recombinant sweet polypeptide), is sufficient to direct transcription of the linked nucleic acid sequence. In some cases, the activity of a promoter can be measured indirectly, such as by assessing the amount of protein produced, where such protein is encoded by the linked nucleic acid (e.g., a gene of interest encoding the heterologous protein).

[0023] Promoters are generally located upstream (5') to the nucleic acid sequence to be transcribed. In some cases, a promoter can be in its native configuration, e.g., operatively linked to a nucleic acid sequence as such linkage is found in nature, e.g., in a native organism. In other cases, such as described herein, a promoter is operatively linked to a heterologous nucleic acid sequence. In some embodiments, a promoter is linked to a heterologous nucleic acid sequence derived from a different organism. For example, a promoter derived from a yeast species is operatively linked to a gene sequence derived from a plant species. In some cases, a promoter derived from one species, such as a promoter derived from one species of filamentous fungi can be used for expression in a different species of filamentous fungi.

[0024] In any of the embodiments provided herein, the promoter can be a constitutive promoter, an inducible promoter, or a hybrid promoter. A hybrid promoter combines a portion of each of at least two different promoters of any kind. In some cases, a promoter is constitutive. In other cases, a promoter is inducible. In some cases, a promoter is a repressible promoter. Exemplary inducible promoters include promoters that are responsive to ethanol, methanol, copper, lactose, or other induction elements. Exemplary repressible promoters include promoters that are responsive to phosphate, glucose, or other repression elements. Promoters are well known in the art and are contemplated by the present application. See, for example, Veeresh, and Wu. ChemBioChem 19.1 (2018), pp. 7-21. Non-limiting examples of promoters are described herein.

[0025] Provided herein are engineered promoters for driving expression of a heterologous protein (e.g., a sweet protein) in a host cell. An engineered promoter (also referred to herein as synthetic promoter) is a non-naturally occurring nucleotide sequence that serves as a promoter for expression of a heterologous gene such as a gene encoding a polypeptide (e.g., a sweet protein). Engineered promoters can includesome or all of the nucleotide sequence of one or more naturally occurring promoters. Engineered promoters can also include one or more mutations (e.g., one, two, three, four, five, six, seven, eight, nine, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, or more mutations) compared to a non-engineered promoter. Engineered promoters also can include combinations of natural promoters or portions thereof and / or engineered promoters or portions thereof to drive expression of a heterologous protein. A mutation can include a base change, a deletion, and / or an insertion of one or more nucleotides compared to any of the promoters described herein. For example, a base change of one or more nucleotides compared to any of the promoters described herein can include a base change of one, two, three, four, five, six, seven, eight, nine, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, or more bases compared to any of the promoters described herein. A deletion of one or more nucleotides compared to any of the promoters described herein can include a deletion of one, two, three, four, five, six, seven, eight, nine, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred or more bases compared to any of the promoters described herein. An insertion of one or more nucleotides compared to any of the promoters described herein can include an insertion of one, two, three, four, five, six, seven, eight, nine, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, or more bases compared to any of the promoters described herein. An engineered promoter can also include the repetition, such as a duplication, of all or a part of a promoter or addition of all or a part of a sequences from a second (different) promoter.

[0026] In some embodiments, an engineered promoter is any promoter set forth in any one of SEQ ID NOs: 3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, or 235-239 (Table 2 or Table 9). In some embodiments, an engineered promoter is a promoter with at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% sequence identity to any one of SEQ ID NOs: 3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, or 235-239. An engineered promoter set forth in any one of SEQ ID NOs: 3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, or 235-239 can include one or more mutations (e.g., one, two, three, four, five, six, seven, eight, nine, ten, twenty, thirty, forty, fifty, sixty, seventy, eighty, ninety, one hundred, or more mutations).

[0027] The provided engineered promoters are recognized by transcription factors and / or RNA polymerase of a host cell and are compatible with expression (e.g., transcription) pathways of a host cell to drive RNA expression that in turn provides production of a heterologous protein, such as a sweet protein. In some cases, the provided engineered promoters are compatible with expression in a fungal cell. In some cases, the provided engineered promoters are compatible with expression in a yeast cell. In somecases, the provided engineered promoters are compatible with a transcription pathway of Komagataella sp. (also known as Pichia sp.), optionally a transcription pathway of Komagataella phaffii cells. In some embodiments, the engineered promoters described herein increase the production of a heterologous protein to which the engineered promoter is operably linked, such as a heterologous sweet protein (e.g., a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof) in a host cell, such as in a yeast cell such as Komagataella sp. (also known as Pichia sp.), optionally Komagataella phaffii. In some cases, the provided engineered promoters increase the production of a heterologous peptide to which it is operably linked compared to a reference promoter or a natural promoter. Such improved production of the heterologous protein increases protein yield upon purification or manufacture.Engineered Signal Peptide

[0028] A signal peptide (also called a secretion signal peptide, a signal sequence, a targeting signal, a localization signal, a localization sequence, a transit peptide, a leader sequence, or a leader peptide) is a peptide sequence present on a newly synthesized polypeptide that is recognized by the secretion pathway of the host cell, ultimately directing the polypeptide across or into a cell membrane of the cell (the plasma membrane in prokaryotes or the endoplasmic reticulum membrane in eukaryotes). Use of signal peptides can help obtain high yields of heterologous proteins, especially secreted heterologous proteins.

[0029] A signal peptide may be present at the N-terminal end of the newly synthesized polypeptide, but in some cases is found elsewhere such as at the C-terminal end or within a polypeptide sequence. In some cases, a signal peptide may contain at least a positively charged N-terminus of 1-5 residues (n-region), a central hydrophobic core of 6-16 amino acids (h-region), and a polar region of 3-7 amino acids that is a recognition site for a signal peptidase (c-region). A signal peptide is typically 15 to 100 amino acids in length. In some cases, a signal peptide is 60 to 80 amino acids in length (e.g., 65 to 85 amino acids). In some cases, the signal peptide is specific to the type of host cell, i.e., promotes secretion only in a subset of host cells.

[0030] All or part of the signal peptide may be cleaved off from the rest of the protein by a signal peptidase, for example, during translocation or shortly after completion of translocation. A signal peptide can be removed (e.g., cleaved by a protease) from the protein such that the mature form of the protein lacks all or a part of the signal peptide. The signal peptide can direct the polypeptide encoding a heterologous protein into a cell's secretory pathway and result in extracellular secretion of theheterologous protein, such as secretion of a heterologous sweet protein. In some cases, a signal peptide can direct a polypeptide into a cell's secretory pathway and result in incorporation of the heterologous protein into the cell's membrane. See, for example, Damasceno and Batt. (2012). Appl Microbiol Biotechnol 93, pp. 31-39, which is incorporated in its entirety herein.

[0031] Extracellular secretion of a recombinantly expressed protein from a host cell can facilitate protein purification. For example, recovery of a recombinant protein from a cell culture supernatant can be preferable to lysing host cells to release a complex mixture of proteins and other substances, including intracellular proteins, carbohydrates and metabolites of the host cell. Secretion, in some cases, can reduce deleterious effects that intracellular overexpression of a heterologous protein can have on a host cell such as toxicity or decreased growth rate. Secretion, in some cases, can allow increased protein production compared to intracellular expression in a host cell of limited volume to store the synthesized proteins. Further, secretory production of a protein, in some cases, can facilitate post-translational modification or processing (e.g., protein folding, formation of disulfide bonds, and glycosylation).

[0032] A signal peptide may be derived from a precursor (e.g., prepropeptide, preprotein) of a known secreted protein. In some cases, the signal peptide is derived from a prepropeptide or preprotein of the host cell. In some cases, the signal peptide is derived from a prepropeptide or preprotein of another type of cell (i.e., not from the host cell). In some cases, the signal peptide is an engineered or synthetic (not naturally occurring) sequence.

[0033] Provided herein are engineered signal peptides and nucleotide sequences encoding the same. In some embodiments, provided herein is a signal peptide set forth in any one of SEQ ID NOs: 66-206. In some embodiments, provided herein is a signal peptide having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% sequence identity with a signal peptide set forth in any one of SEQ ID NOs: 66-206.

[0034] Engineered signal peptides are non-naturally occurring amino acid sequences that are recognized by the secretory pathway of the host cell and facilitate translocation of a heterologous protein into the secretory pathway and ultimately to extracellular secretion of the heterologous protein. In some cases, the provided engineered signal peptides are compatible with the secretory pathway of a filamentous fungal cell. In some cases, the provided engineered signal peptides are compatible with the secretory pathway of Komagataella sp. cells, optionally Komagataella phaffii cells. In some cases, the provided engineered signal peptides facilitate secretion of the heterologous protein (e.g., sweet protein) into culture medium in which the host cell is grown, fermented, or propagated.

[0035] Engineered signal peptides can include one or more mutations (e.g., one, two, three, four, five, six, seven, eight, nine, ten, fifteen, twenty, twenty-five, thirty, thirty-five, forty, forty-five, fifty or more mutations) compared to any of the signal peptides that are naturally occurring or otherwise known. A mutation can include an amino acid substitution, a deletion, and / or an insertion of one or more amino acids. Engineered signal peptides also can include combinations of one or more natural signal peptide(s) or fragment(s) thereof and / or one or more engineered signal peptide(s) or fragment(s) thereof.

[0036] In some cases, a signal peptide can include a truncated signal peptide or a fragment of a known signal peptide. In some cases, truncated signal peptides have maintained their secretory function. For example, a truncated signal peptide can be 15 to 25 amino acids long and includes a functional portion of the non-truncated signal peptide. An insertion of one or more amino acids compared to any of the signal peptides described herein can include an insertion of one, two, three, four, five, six, seven, eight, nine, ten, fifteen, twenty, twenty-five, thirty, thirty-five, forty, forty-five, fifty or more amino acids compared to any of the signal peptides described herein. An engineered signal peptide can also include insertion of all or a part of a duplicate signal peptide or insertion of all or a part of one or more additional signal peptides (e.g., a second signal peptide) before, within, or after a signal peptide described.

[0037] For example, a substitution of one or more amino acids compared to any of the signal peptides described herein can include an amino acid change of one, two, three, four, five, six, seven, eight, nine, ten, fifteen, twenty, twenty-five, thirty, thirty-five, forty, forty-five, fifty or more amino acids compared to any of the signal peptides described herein. A deletion of one or more amino acids compared to any of the signal peptides described herein can include a deletion of one, two, three, four, five, six, seven, eight, nine, ten, fifteen, twenty, twenty-five, thirty, thirty-five, forty, forty-five, fifty or more amino acids compared to any of the signal peptides described herein. In some embodiments, provided herein is an engineered signal peptide as set forth in any one of SEQ ID NOs: 67, 75, 77, 81, 88, 99-107, 109-200, or 203-206. In some embodiments, provided herein is an engineered signal peptide having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% sequence identity with a signal peptide set forth in any one of SEQ ID NOs: 67, 75, 77, 81, 88, 99-107, 109-200, or 203-206.

[0038] The signal peptides described herein, including the engineered signal peptides described herein, can be fused to a heterologous protein, such as a sweet protein to promote extracellular secretion of the mature heterologous protein, such as a sweet protein. In some embodiments, a nucleotide sequence encoding a signal peptide is fused in frame to the 5' end of a heterologous protein, such as a sweet protein(e.g., a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof).

[0039] In some embodiments, the engineered signal peptides described herein increase the extracellular secretion of a heterologous protein, such as a sweet protein (e.g., a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof). In some cases, the engineered signal peptides increase the efficiency of the extracellular secretion of the heterologous protein, such as a sweet protein. In some cases, the provided signal peptides increase the secretion or efficiency of the secretion of a heterologous peptide compared to a reference signal peptide. In some cases, the provided signal peptide facilitates the entry of the heterologous protein into the secretory pathway of the host cell. Such improved secretion increases protein yield upon production of the heterologous protein.Expression Cassettes

[0040] Expression cassettes can include expression elements (e.g., promoters, signal peptides, and transcription terminators) that are combined with the coding sequence of a heterologous protein to express, and optionally secrete, a heterologous protein, such as a sweet protein. For example, an expression cassette can include a first nucleotide sequence encoding any promoter described herein operably linked to a coding sequence of any heterologous protein (e.g., a sweet polypeptide), or any combination of promoters described herein operably linked to a coding sequence of any heterologous protein (e.g., a sweet polypeptide). Exemplary promoters that can be included in the expression cassettes described herein include, but are not limited to, an ACO1, acu-5, adhl+, alcohol dehydrogenase (ADH1, ADH2, ADH3, ADH4), AHSB4m, AINV, alcA, a-amylase, ALD4, alternative oxidase (AOD), alcohol oxidase 1 (AOX1), alcohol oxidase 2 (AOX2), ATO, ATP fragment (e.g., ATP1 or ATP2), AXDH, B2, CaMV, cellobiohydrolase 1 (cbhl), CBOI, ccg-1, cDNAl, cellular filament polypeptide (cfp), CIT, cpc-2, CTA1, ctr4+, CUP1, dihydroxyacetone synthase (DAS), enolase (ENO, ENO1), EPX1, EXG1-1000, FDH1, formaldehyde dehydrogenase (FLD1), FMD, formate dehydrogenase (FMDH), Gl, G6, GAA, GALI, GAL2, GAL3, GAL4, GAL5, GAL6, GAL7, GAL8, GALS, GAL10, GAP, GCW14, gdhA, gla-1, a-glucoamylase (glaA), glyceraldehydes’ phosphate dehydrogenase (gpdA, GAP, GAPDH), phosphoglycerate mutase (GPM1), GTH1, glycerol kinase (GUT1), heat shock protein (e.g., HSP10, HSP12, HSP82), invl, isocitrate lyase (ICL1), acetohydroxy acid isomeroreductase (ILV5), KAR2, KCAP, KEX2, KKUR, KP1113, KPAS, b-galactosidase (Iac4), LEET2, melO, MET3, MLS1, methanol oxidase (MOX), nmtl, NSP, 0LE1, OPOL, PCK1, PDC1, PDI1, pcbC, PET9,peroxin 8 (PEX8), phosphoglycerate kinase (PGK, PGK1), phol, PH05, PHO89, phosphatidylinositol synthase (PIS1), PIR1, PKUD, PYK1, pyruvate kinase (pkil), PMEM, PORI, QCR7, RGI2, RPS7, SCW10, sorbitol dehydrogenase (SDH), SD001, 3-phospho serine aminotransferase (SERI), SINS, SSA3, SSA4, SV40, TDH3, TEF, translation elongation factor 1 alpha (TEF1), THI11, homoserine kinase (THR1), TMA10, tpi, TPS1, triose phosphate isomerase (TPI1), XRP2, or YPT1 promoter, or functional fragments thereof, or any combination of the full-length promoters and / or fragments thereof.

[0041] In some cases, the promoter in the expression cassette is any one of SEQ ID NOs: 1-65, or 230-239. In some cases, the promoter is any one of the promoters set forth in Table 2. In some cases, a promoter is any promoter set forth in Table 3. In some cases, a promoter is any promoter set forth in Table 4. In some cases, the promoter is any promoter set forth in Table 9. In some cases, the promoter in the expression cassette is any promoter set forth in any one of SEQ ID NOs: 1-34, 65, or 230-239.

[0042] In some cases, the promoter in the expression cassette is an engineered promoter described herein. In some cases, the promoter in the expression cassette is any promoter set forth in any one of SEQ ID NOs: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, 65, 232, 233, 234, 235, 236, 237, and 238. In some cases, the promoter in the expression cassette is an engineered promoter with at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% sequence identity to any one of SEQ ID NOs: 3-8, 10- 13, 18, 26-32, 34, 56, 58, 65, 230-233, or 235-239.

[0043] In some cases, an expression cassette can include a first nucleotide sequence encoding any promoter set forth in any one of SEQ ID NOs: 1-65 operably linked to a coding sequence of a heterologous protein (e.g., a sweet protein). In some cases, an expression cassette can include a first nucleotide sequence encoding any promoter set forth in Tables 2, 3, or 4 operably linked to a coding sequence of a heterologous protein (e.g., a sweet protein). In some cases, an expression cassette can include a first nucleotide sequence encoding any promoter set forth in any one of SEQ ID NOs: 1-34, or 65 operably linked to a coding sequence of a heterologous protein (e.g., a sweet protein). In some cases, an expression cassette can include a first nucleotide sequence that is a promoter (e.g., an engineered promoter) set forth in anyone of SEQ ID NOS: 3-8, 10-13, 18, 26-32, 34, 56, 58, or 65 operably linked to a coding sequence of a heterologous protein (e.g., a sweet protein).

[0044] Expression cassettes can further include a second nucleotide sequence encoding any signal peptide described herein or combination of signal peptides described herein. In some cases, a second nucleotide sequence encoding a signal peptide can be linked in-frame to a coding sequence of a heterologous protein (e.g., a coding sequence of a sweet protein or a coding sequence of a sweet proteinfragment or peptide). A signal peptide can be in any appropriate location such that the associated polypeptide sequence is secreted from a host cell. In some cases, a second nucleotide sequence encoding a signal peptide can be upstream of a coding sequence of a heterologous protein (e.g., a coding sequence of a sweet protein). In some cases, a second nucleotide sequence encoding a signal peptide can be downstream of a promoter sequence and upstream of a coding sequence of a heterologous protein (e.g., a coding sequence of a sweet protein). In some cases, a second nucleotide sequence encoding a signal peptide can be 5' to a heterologous protein coding sequence (e.g., a coding sequence of a sweet polypeptide). In some cases, a second nucleotide sequence encoding a signal peptide can be 3' to the coding sequence of the heterologous protein (e.g., a coding sequence of a sweet protein). In some cases, a second nucleotide sequence encoding a signal peptide can be 5' and / or 3' to the coding sequence of the heterologous protein described herein (e.g., a coding sequence of a sweet protein or fragment).

[0045] In some cases, an expression vector includes a combination of a promoter and a signal peptide, particularly any of the combination of promoter and signal peptides listed in Table 11. In such cases, the promoter is operably linked to the coding sequence of a heterologous protein, such as a sweet protein. The signal peptide is encoded by a nucleic acid in-frame with the coding sequence of the heterologous protein (e.g., the sweet protein), such that when expressed, the signal peptide is operably linked to the heterologous protein, and then may be cleaved after expression. In some aspects, the sweet protein is any one of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof. In some aspects, the sweet protein has an amino acid sequence of any one of SEQ ID Nos: 208, 211-229.

[0046] Expression cassettes including a second nucleotide sequence encoding any signal peptide described herein can be linked to a coding sequence of a heterologous protein, such as the coding sequence of a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide upstream of (and in-frame with) a coding sequence of a heterologous protein (e.g., a coding sequence of a sweet protein, such as a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof). In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide downstream (and in-frame with) of a coding sequence of a heterologous protein (e.g., a coding sequence of a sweet protein, such as brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof). In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide downstreamof any promoter sequence described herein and upstream of a coding sequence encoding a heterologous protein (e.g., a coding sequence of a sweet protein). In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide downstream of any promoter sequence described herein and downstream of a coding sequence encoding a heterologous protein (e.g., a coding sequence of a sweet protein). In some cases, an expression cassette can include a second nucleic acid sequence encoding any signal peptide described herein fused in frame to a coding sequence for a heterologous protein (e.g., a coding sequence of a sweet protein).

[0047] In some cases, an expression cassette can include a second nucleic acid sequence encoding any signal peptide set forth in any one of SEQ ID NOS: 66-206 or set forth in Tables 2, or 5-8 can be fused in frame to a coding sequence for a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOS: 66-206 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 66, 81, 82, 97, 144, 145, 149, 150, 155, 159, 166, 174, and 189-200 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 81, 82, 97, 144, 155, 190, and 195 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 100, 106, 107, 109, 113-116, 119, 120, 122, 125, 127, 149, 155, 158, and 203 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 100, 109, 115, 119, 120, 149, and 158 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 67-71, 79, 81, 85, 116, 134, and 150 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 69, 79, 81, and 85 can be upstream of a heterologous protein, such as a sweet protein. In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 67, 75, 77, 81, 88, 99-107, 109-200, or 203-206 can be upstream of a heterologous protein, such as a sweet protein.

[0048] In some cases, an expression cassete can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 66-206 can be downstream of a first nucleotide sequence encoding any promoter set forth in any one of SEQ ID NOs: 1-65 and upstream of a coding sequence encoding a heterologous protein, such as a sweet protein (e.g., a coding sequence of a sweet polypeptide). In some cases, an expression cassette can include a second nucleotide sequence encoding any signal peptide set forth in any one of SEQ ID NOs: 66-206 can be downstream of a first nucleotide sequence encoding any promoter described herein (e.g., an engineered promoter, such as a promoter set forth in any one of SEQ ID NOS: 3-8, 10-13, 18, 26-32, 34, 56, 58, or 65) and upstream of a coding sequence encoding a heterologous protein (e.g., a coding sequence of a sweet protein or functional fragment thereof).

[0049] In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide can be downstream of any promoter (e.g., an engineered promoter) set forth in any one of SEQ ID NOS: 3-8, 10-13, 18, 26-32, 34, 56, 58, or 65 and upstream of a coding sequence encoding a heterologous protein (e.g., a coding sequence of a sweet protein or functional fragment thereof). In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide can be downstream of any promoter (e.g., an engineered promoter) set forth in any one of SEQ ID NOS: 1-34 or 65 and upstream of a coding sequence encoding a heterologous protein (e.g., a coding sequence of a sweet protein or functional fragment thereof).

[0050] In some cases, an expression cassette can include a second nucleotide sequence encoding a signal peptide can be downstream of any promoter (e.g., an engineered promoter) set forth in any one of SEQ ID NOS: 3-8, 10-13, 18, 26-32, 34, 56, 58, or 65 and upstream of a coding sequence encoding a heterologous protein, such as a sweet protein (e.g., a coding sequence of a sweet protein such as brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof). In some cases, an expression cassete can include a second nucleotide sequence encoding a signal peptide can be downstream of any promoter (e.g., an engineered promoter) set forth in any one of SEQ ID NOS: 1-34 or 65 and upstream of a coding sequence encoding a heterologous protein (e.g., a coding sequence of a sweet protein such as brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof).

[0051] Expression cassetes can further include one or more transcription terminators (also called terminators). A transcription terminator indicates the end of a gene or operon in genomic DNA during transcription. The transcription terminator sequence provides a signal in the newly synthesized transcriptRNA that causes the transcriptional complex to release the RNA transcript. These processes include the direct interaction of the mRNA secondary structure with the complex and / or the indirect activities of recruited termination factors. Exemplary transcription terminators include the DHAS1 transcription terminator and the A0X1 transcription terminator. In some cases, the expression cassette includes a transcription terminator having the sequence set forth in SEQ ID NO: 209 or 210.

[0052] Expression cassettes can be incorporated into vectors (also called expression vectors). Expression cassettes and / or vectors can also be incorporated into host cells to facilitate production of a heterologous peptide (e.g., a sweet polypeptide).Vectors

[0053] Described herein are vectors including a nucleic acid sequence encoding a heterologous protein that can be used to produce the heterologous protein. A vector is a DNA molecule (often plasmid or virus) that is used as a vehicle to carry a particular DNA segment into a host cell as part of a cloning or recombinant DNA technique. The vector typically assists in replicating and / or expressing the inserted DNA sequence inside the host cell. Vectors can include origins of replication and partitioning systems to facilitate replication in a host cell. In some cases, the vector provides for low copy number of the vector in the host cell. In some cases, the vector provides for high copy number of the vector in the host cell. In some cases, the vector does not replicate in the host cell and is used to introduce the nucleic acid sequences of interest (e.g., one or more expression cassettes) into the host cell for integration in the genome. In some cases, integration into the genome is by homologous recombination. In some cases, integration into the genome is by non-homologous recombination (e.g., random integration). Integration into the genome of a host cell can include single copy integration or can include integration of multiple copies of one or more vectors or expression cassettes. Included among the provided vectors are multicistronic vectors, such a bicistronic vectors.

[0054] In some embodiments, provided herein are vectors that include a nucleic acid sequence encoding a recombinant sweet protein (e.g., a nucleic acid encoding a recombinant brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof) operably linked to one or more of the promoters, signal peptides, or a combination thereof. An appropriate vector can be chosen for the preferred host cell. An appropriate vector would be maintained in the host cell long enough for production and / or secretion of a heterologous protein (e.g., a sweet protein), or for integration of the nucleic acid sequence encoding a recombinant sweet protein.

[0055] Described herein are vectors for production of a heterologous protein, such as a sweet protein. A vector containing a polynucleotide encoding a heterologous protein (e.g., a sweet protein) can also include any expression cassette described herein. A vector containing a polynucleotide encoding a heterologous protein (e.g., a sweet protein) can also include a first nucleotide sequence encoding any of the promoters described herein. A vector containing a gene encoding any heterologous protein described herein (e.g., a sweet polypeptide) can include a polynucleotide sequence encoding any signal peptide disclosed herein.

[0056] In some cases, a vector includes an expression cassette including a first nucleotide sequence encoding a promoter operably linked to a protein coding sequence encoding any of the heterologous proteins disclosed here. In some cases, a vector includes an expression cassette that also includes a second nucleotide sequence encoding any of the signal peptides disclosed herein. In some cases, a vector includes an expression cassette including a first nucleotide sequence encoding a promoter operably linked to a protein coding sequence encoding any of the heterologous proteins disclosed here and a second nucleotide sequence encoding any of the signal peptides disclosed herein. In some cases, a second nucleotide sequence encoding any of the signal peptides disclosed herein can be located 5' of the protein coding sequence or 3' of the protein coding sequence. In some cases, a second nucleotide sequence encoding any of the signal peptides disclosed herein can be located 3' of the first nucleotide sequence encoding any of the promoters disclosed herein and 5' of the protein coding sequence.

[0057] In some cases, a vector contains more than one expression cassette, such as two or more (e.g., two, three, four, five, or six or more) of any of the expression cassettes described herein.Host Cells

[0058] Described herein are cells that contain one or more of the promoters described herein, one or more of the expression cassettes described herein, one or more of the signal peptides described herein, one or more of the signal peptides herein fused to a heterologous protein (e.g., a heterologous sweet protein), and / or one or more of the vectors described herein that can be used to produce and / or secrete a heterologous protein, such as a sweet protein. The expression of the heterologous protein, such as a sweet protein, can be transient or stable. In some examples the expression of the heterologous protein is stable (i.e., maintained over subsequent generations or period of host cell growth). In some preferred cases, the provided cells contain one or more of the described expression cassettes stably integrated into the genome of the cell. Such stable integration is routine, such as by homologous recombination or non-homologous recombination (e.g., random integration). In some examples, two or more expression cassettes (e.g., two, three, four, five, or six or more expression cassettes) are integrated into the host cell genome. In some examples the two or more expression cassettes are integrated into the same locus in the host cell genome. In some examples, the two or more expression cassettes are integrated into different loci in the host cell genome. Integration into the genome of a host cell can include single copy integration or can include integration of multiple copies of one or more vectors or expression cassettes.

[0059] As different host cells can have characteristics and specific mechanisms for the posttranslational processing and modification of protein products, appropriate cell lines or host systems can be chosen to ensure the desired modification and processing of a heterologous proteins. In some cases, a host cell can be a eukaryotic cell (e.g., fungal cell). A host cell can be grown, fermented, propagated, or cultured in any appropriate medium that allows for host cell growth and expression of any sweet polypeptide.

[0060] In some cases, a host cell selected for expression of a heterologous protein (e.g., a sweet polypeptide) can be a fungal cell. The fungal cell can be a yeast cell or a filamentous fungus cell. In some cases, the host cell is a yeast cell. In some cases, the host cell is a filamentous fungus. Yeast cells can include, but are not limited to, Arxula spp., Arxula adeninivorans, Kluyveromyces spp., Kluyveromyces lactis, Pichia spp., Pichia angusta, Pichia pastoris (also known as Komagataella phaffii), Saccharomyces spp., Saccharomyces cerevisiae, Schizosaccharomyces spp., Schizosaccharomyces pombe, Hansenula sp., Blastobotrys sp., Candida sp., Zygosaccharomyces sp., Debaryomyces sp. Yarrowia spp., and Yarrowia lipolytica cells. Fungal cells can include, but are not limited to, Agaricus spp., Agaricus bisporus, Aspergillus spp., Aspergillus awamori, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Blastobotrys sp., Colletotrichum spp., Colletotrichum gloeosporiodes, Endothia spp., Endothia parasitica, Fusarium spp., Fusarium graminearum, Fusarium solani, Mucor spp., Mucor miehei, Mucor pusillus, Myceliophthora spp., Myceliophthora thermophila, Neurospora spp., Neurospora crassa, Penicillium spp., Penicillium camemberti, Penicillium canescens, Penicillium chrysogenum, Penicillium (Talaromyces) emersonii, Penicillium funiculo sum, Penicillium purpurogenum, Penicillium roqueforti, Pleurotus spp., Pleurotus ostreatus, Rhizomucor spp., Rhizomucor miehei, Rhizomucor pusillus, Rhizopus spp., Rhizopus arrhizus, Rhizopus oligosporus, Rhizopus oryzae, Trichoderma spp., Trichoderma atroviride, Trichoderma reesei, and Trichoderma vireus cells.

[0061] In some cases, the host cell for expression of any sweet polypeptide disclosed herein is a Pichia spp., such as Pichia pastoris. In some cases, the host cell for expression of any recombinant sweetpolypeptide is Pichia pastoris. In some cases, the host cell for expression of recombinant brazzein-53 is a Pichia spp., such as Pichia pastoris.

[0062] The methylotrophic yeast Pichia pastoris (also called Komagataella phaffii) is known to be safe and is widely used for recombinant protein expression suitable for industrial production. This system allows high-level expression, proper folding, and post-translational modifications such as phosphorylation and glycosylation under the control of a promoter. In addition, proteins can be secreted by the yeast P. pastoris at high levels in minimal medium, facilitating purification from the culture supernatant and reducing downstream costs. The natural secretion of proteins is also advantageous in that the naturally- secreted protein possesses an N-terminus identical to the natural protein if the signal peptide is properly cleaved.ii. Sweet Proteins as Heterologous Proteins

[0063] The promoters, signal peptides, expression cassettes, vectors, and cells provided herein can be used to produce or manufacture a heterologous protein. In some preferred embodiments, the heterologous protein is a sweet protein (also referred to herein as a "sweet polypeptide"). A sweet protein is a polypeptide capable of interacting with the human sweet taste receptor. The expression components and methods provided herein can be employed to produce and / or secrete any identified sweet protein. Several naturally occurring sweet proteins have been identified and isolated from a variety of plants, fruits, and fungi. Exemplary natural and mutant sweet proteins are described in Kant Nutr J. 2005 Feb 9;4:5; Ming D et al., FEBS Lett. (1994) 355:106-108; WO2019 / 173541; WO2021 / 263158; and WO2023 / 129938, all of which are incorporated by reference in their entirety and are included among the sweet proteins described herein. Non-limiting examples of sweet proteins include a recombinant brazzein polypeptide or a functional fragment thereof (e.g., recombinant brazzein-53), a recombinant monellin polypeptide or a functional fragment thereof, a recombinant miraculin polypeptide or a functional fragment thereof, a recombinant mabinlin polypeptide or a functional fragment thereof, a recombinant curculin polypeptide or a functional fragment thereof, a recombinant thaumatin polypeptide or a functional fragment thereof, a recombinant mycodulcein polypeptide or a functional fragment thereof. In some cases, the heterologous protein is a brazzein or a functional fragment thereof, a brazzein-54 or a functional fragment thereof, a brazzein-53 or a functional fragment thereof. In some cases, the heterologous protein is a monellin or a functional fragment thereof. In some cases, the heterologousprotein is a thaumatin or a functional fragment thereof, a thaumatin I or a functional fragment thereof, or a thaumatin II or a functional fragment thereof. Exemplary sweet protein amino acid sequences are provided in Table 1. Sweet proteins also include variants of any of the described sweet proteins that retain the ability to interact with the human taste receptor and impart a sweet taste upon consumption.

[0064] The terms "polypeptide," "peptide," and "protein" are used interchangeably herein to refer to polymers of amino acids of at least 5 amino acids long. The polymer can be linear or branched, it can include modified amino acids (e.g., pyroglutamate (pGlu)), and / or it can be interrupted by non-amino acids. The terms also encompass an amino acid polymer that has been modified, for example, disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation, such as conjugation with a labeling component or a tag.

[0065] In some embodiments, the sweet protein is a brazzein protein or a functional fragment thereof. In some embodiments, the sweet protein is a Brazzein-54 protein. In some embodiments, the sweet protein is a Brazzein-53 protein. Amino acid sequences of exemplary brazzein proteins are set forth in SEQ ID NOS: 214 and 215. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 1. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 2. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 6. In someembodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 12. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 18. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the codingsequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 34. In some embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the coding sequence of the Brazzein protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0066] In any of the provided embodiments, the sweet protein is a Brazzein protein, such as Brazzein-53 or Brazzein-54, and the expressed Brazzein protein is fused to a signal peptide. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Brazzein protein, such asBrazzein-53 or Brazzein-54, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66, 81, 82, 97, 144, 145, 149, 150, 155, 159, 166, 174, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, or 200. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 81, 82, 97, 144, 145, 155, 189, 190, or 195. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 66. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 81. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein- 54, is fused to a signal peptide set forth in SEQ ID NO: 97. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 144. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 145. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 149. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 150. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein- 54, is fused to a signal peptide set forth in SEQ ID NO: 155. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 159. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 166. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 174. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 189. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein- 54, is fused to a signal peptide set forth in SEQ ID NO: 190. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 191. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 192. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 193. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 194. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 195. In some embodiments, the expressed Brazzeinprotein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 196. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 197. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 198. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused to a signal peptide set forth in SEQ ID NO: 199. In some embodiments, the expressed Brazzein protein, such as Brazzein-53 or Brazzein- 54, is fused to a signal peptide set forth in SEQ ID NO: 200.

[0067] In some embodiments, the coding sequence of a Brazzein protein, such as Brazzein-53 or Brazzein- 54, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66, 81, 82, 97, 144, 145, 149, 150, 155, 159, 166, 174, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, or 200 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Brazzein protein, such as Brazzein-53 or Brazzein- 54, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66, 81, 82, 97, 144, 145, 149, 150, 155, 159, 166, 174, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, or 200 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the coding sequence of a Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 81, 82, 97, 144, 145, 155, 189, 190, or 195 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Brazzein protein, such as Brazzein-53 or Brazzein-54, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 81, 82, 97, 144, 145, 155, 189, 190, or 195 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.

[0068] In some embodiments, the sweet protein is a monellin protein or a functional fragment thereof. Amino acid sequences of an exemplary monellin protein are set forth in SEQ ID NOS: 216, 217, and 229. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin proteinis operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 1. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 2. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 6. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 12. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 18. In someembodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operabiy linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operabiy linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operabiy linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operabiy linked to a promoter set forth SEQ ID NO: 34. In some embodiments, the sweet protein is a Monellin protein, and the coding sequence of the Monellin protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0069] In any of the provided embodiments, the sweet protein is a Monellin protein, and the expressed Monellin protein is fused to a signal peptide. In some embodiments, the expressed Monellin protein, isfused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 100, 106, 107, 109, 113, 114, 115, 116, 119, 120, 122, 125, 127, 149, 155, 158, or 203. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 100, 109, 115, 119, 120, 149, or 158. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 113. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 114. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 115. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 116. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 119. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 120. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 122. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 125. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 127. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 149. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 155. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 158. In some embodiments, the expressed Monellin protein, is fused to a signal peptide set forth in SEQ ID NO: 203.

[0070] In some embodiments, the coding sequence of a Monellin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 100, 106, 107, 109, 113, 114, 115, 116, 119, 120, 122, 125, 127, 149, 155, 158, or 203 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Monellin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 100, 106, 107, 109, 113, 114, 115, 116, 119, 120, 122, 125, 127, 149, 155, 158, or 203 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25,33, 34, or 65. In some embodiments, the coding sequence of a Monellin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 100, 109, 115, 119, 120, 149, or 158 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Monellin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 100, 109, 115, 119, 120, 149, or 158 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.

[0071] In some embodiments, the sweet protein is a miraculin protein or a functional fragment thereof. An amino acid sequence of an exemplary miraculin protein is set forth in SEQ ID NO: 218. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 1. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 2. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 6. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin proteinis operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 12. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 18. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is aMiraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 34. In some embodiments, the sweet protein is a Miraculin protein, and the coding sequence of the Miraculin protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0072] In any of the provided embodiments, the sweet protein is a Miraculin protein, and the expressed Miraculin protein is fused to a signal peptide. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 66. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 67. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 68. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 69. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 70. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 71. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 72. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 73 In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 74. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 75. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 76. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 77. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 78. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 79. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 80. In someembodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 81. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 83. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 84. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 85. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 86. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 87. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 90. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 91. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 92. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 93. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 94. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 95. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 96 In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 97. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 98. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 99. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide setforth in SEQ ID NO: 107. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 110. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 111. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 112. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 113. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 114. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 115. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 116. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 117. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 118. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 119. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 120. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 121. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 122. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 123. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 124. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ IDNO: 125. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 126. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 127. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 128. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 129. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 130. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 131. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 132. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 133. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 134. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 135. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 136. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 137. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 138. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 139. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 140. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 141. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 142. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 143. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 144. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 145. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 146. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 147. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 148. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 149. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 150. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 151. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 152. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 153. In someembodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 154. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 155. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 156. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 157. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 158. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 159. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 160. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 161. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 162. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 163. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 164. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 165. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 166. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 167. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 168. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 169. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 170. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 171. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 172. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 173. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 174. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 175. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 176. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 177. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 178. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 179. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 180. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 181. In some embodiments, the expressedMiraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 182. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 183. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 184. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 185. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 186. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 187. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 188. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 189. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 190. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 191. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 192. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 193. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 194. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 195. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 196. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 197. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 198. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 199. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 200. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 201. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 202. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 203. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 204. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 205. In some embodiments, the expressed Miraculin protein, is fused to a signal peptide set forth in SEQ ID NO: 206.

[0073] In some embodiments, the coding sequence of a Miraculin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Miraculin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.

[0074] In some embodiments, the sweet protein is a mabinlin protein or a functional fragment thereof. Exemplary amino acid sequences for mabinlin proteins are set forth in SEQ ID NOS: 219-225. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 1. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 2 In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 6. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 12. In someembodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 18. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin proteinis operably linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 34. In some embodiments, the sweet protein is a Mabinlin protein, and the coding sequence of the Mabinlin protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0075] In any of the provided embodiments, the sweet protein is a Mabinlin protein, and the expressed Mabinlin protein is fused to a signal peptide. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 66. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 67. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 68. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 69. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 70. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 71. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 72. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 73 In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 74. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 75. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 76. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 77. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 78. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 79. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 80. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 81. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ IDNO: 82. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 83. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 84. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 85. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 86. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 87. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 90. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 91. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 92. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 93. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 94. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 95. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 96 In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 97. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 98. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 99. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth inSEQ ID NO: 101. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 110. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 111. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 112. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 113. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 114. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 115. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 116. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 117. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 118. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 119. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 120. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 121. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 122. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 123. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 124. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 125. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 126. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 127. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 128. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 129. In some embodiments, the expressed Mabinlin protein, isfused to a signal peptide set forth in SEQ ID NO: 130. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 131. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 132. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 133. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 134. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 135. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 136. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 137. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 138. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 139. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 140. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 141. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 142. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 143. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 144. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 145. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 146. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 147. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 148. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 149. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 150. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 151. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 152. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 153. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 154. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 155. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 156. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 157. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 158. In someembodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 159. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 160. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 161. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 162. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 163. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 164. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 165. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 166. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 167. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 168. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 169. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 170. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 171. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 172. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 173. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 174. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 175. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 176. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 177. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 178. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 179. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 180. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 181. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 182. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 183. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 184. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 185. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 186. In some embodiments, the expressed Mabinlin protein, is fused to a signalpeptide set forth in SEQ ID NO: 187. in some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 188. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 189. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 190. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 191. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 192. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 193. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 194. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 195. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 196. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 197. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 198. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 199. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 200. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 201. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 202. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 203. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 204. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 205. In some embodiments, the expressed Mabinlin protein, is fused to a signal peptide set forth in SEQ ID NO: 206.

[0076] In some embodiments, the coding sequence of a Mabinlin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Mabinlin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.

[0077] In some embodiments, the sweet protein is a curculin protein or a functional fragment thereof. Exemplary amino acid sequences for curculin proteins are set forth in SEQ ID NOS: 226 and 227. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein isoperably linked to a promoter set forth in any of SEQ ID NOS:: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 1. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 2. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 6. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 12. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is aCurculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 18. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Curculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 34. In some embodiments, the sweet protein is aCurculin protein, and the coding sequence of the Curculin protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0078] In any of the provided embodiments, the sweet protein is a Curculin protein, and the expressed Curculin protein is fused to a signal peptide. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 66. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 67. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 68. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 69. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 70. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 71. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 72. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 73 In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 74. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 75. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 76. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 77. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 78. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 79. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 80. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 81. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 83. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 84. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 85. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 86. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 87. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forthin SEQ ID NO: 88. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 90. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 91. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 92. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 93. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 94. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 95. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 96 In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 97. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 98. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 99. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Curculin protein, isfused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Curculin protein, isfused to a signal peptideset forth in SEQ ID NO: 107. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 110. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 111. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 112. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 113. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 114. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 115. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 116. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 117. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 118. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 119. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 120. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 121. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 122. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 123. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 124. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 125. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 126. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 127. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 128. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 129. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 130. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 131. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 132. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 133. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 134. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 135. In some embodiments, the expressed Curculin protein, is fusedto a signal peptide set forth in SEQ ID NO: 136. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 137. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 138. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 139. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 140. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 141. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 142. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 143. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 144. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 145. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 146. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 147. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 148. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 149. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 150. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 151. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 152. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 153. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 154. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 155. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 156. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 157. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 158. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 159. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 160. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 161. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 162. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 163. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 164. In some embodiments, the expressedCurculin protein, is fused to a signal peptide set forth in SEQ ID NO: 165. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 166. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 167. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 168. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 169. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 170. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 171. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 172. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 173. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 174. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 175. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 176. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 177. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 178. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 179. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 180. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 181. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 182. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 183. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 184. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 185. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 186. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 187. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 188. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 189. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 190. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 191. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 192. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 193. In someembodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 194. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 195. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 196. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 197. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 198. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 199. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 200. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 201. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 202. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 203. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 204. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 205. In some embodiments, the expressed Curculin protein, is fused to a signal peptide set forth in SEQ ID NO: 206.

[0079] In some embodiments, the coding sequence of a Curculin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Curculin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.

[0080] In some embodiments, the sweet protein is a thaumatin protein or a functional fragment thereof. In some embodiments, the sweet protein is a Thaumatin I protein. In some embodiments, the sweet protein is a Thaumatin II protein. Amino acid sequences of exemplary Thaumatin proteins are set forth in SEQ ID NOS: 212 and 213. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 1. Insome embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 2. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 6. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 12. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 18. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence ofthe Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 34. In some embodiments, the sweet protein is a Thaumatin protein, and the coding sequence of the Thaumatin protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0081] In any of the provided embodiments, the sweet protein is a Thaumatin protein, and the expressed Thaumatin protein is fused to a signal peptide. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 67, 68, 69, 70, 71, 79, 81, 85, 116, 134, or 150. In some embodiments, the expressed Thaumatin protein,is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 69, 79, 81, or 85. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 67. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 68. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 69. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 70. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 71. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 79. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 81. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 85. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 116. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 134. In some embodiments, the expressed Thaumatin protein, is fused to a signal peptide set forth in SEQ ID NO: 150.

[0082] In some embodiments, the coding sequence of a Thaumatin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 67, 68, 69, 70, 71, 79, 81, 85, 116, 134, or 150 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Thaumatin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 67, 68, 69, 70, 71, 79, 81, 85, 116, 134, or 150 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the coding sequence of a Thaumatin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 69, 79, 81, or 85 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Thaumatin protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 69, 79, 81, or 85 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.

[0083] In some embodiments, the sweet protein is a mycodulcein protein. Exemplary amino acid sequences for mycodulcein proteins are set forth in SEQ ID NO: 228. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linkedto a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 1. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 2 In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 3. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 4. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 5. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 6. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 7. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 8. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 9. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 10. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 11. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 12. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 13. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 14. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 15. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to apromoter set forth SEQ ID NO: 16. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 17. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 18. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 19. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 20. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 21. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 22. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 23. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 24. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 25. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 26. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 27. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 28. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 29. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 30. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 31. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 32. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 33. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forthSEQ ID NO: 34. In some embodiments, the sweet protein is a Mycodulcein protein, and the coding sequence of the Mycodulcein protein is operably linked to a promoter set forth SEQ ID NO: 65.

[0084] In any of the provided embodiments, the sweet protein is a Mycodulcein protein, and the expressed Mycodulcein protein is fused to a signal peptide. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in any one or more of SEQ ID NOS: 66-206. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 66. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 67. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 68. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 69. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 70. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 71. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 72. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 73 In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 74. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 75. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 76. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 77. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 78. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 79. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 80. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 81. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 82. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 83. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 84. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 85. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 86. In some embodiments, the expressed Mycodulcein protein, is fused to a signalpeptide set forth in SEQ ID NO: 87. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 88. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 90. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 91. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 92. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 93. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 94. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 95. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 96 In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 97. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 98. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 99. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 105. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 100. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 101. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 102. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 103. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 104. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forthin SEQ ID NO: 105. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 106. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 107. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 108. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 109. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 110. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 111. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 112. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 113. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 114. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 115. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 116. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 117. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 118. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 119. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 120. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 121. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 122. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 123. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 124. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 125. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 126. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 127. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 128. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 129. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 130. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 131. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 132. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 133.In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 134. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 135. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 136. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 137. in some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 138. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 139. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 140. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 141. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 142. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 143. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 144. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 145. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 146. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 147. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 148. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 149. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 150. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 151. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 152. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 153. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 154. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 155. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 156. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 157. in some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 158. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 159. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 160. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 161. In some embodiments, theexpressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 162. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 163. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 164. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 165. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 166. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 167. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 168. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 169. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 170. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 171. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 172. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 173. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 174. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 175. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 176. In some embodiments, the expressed Mycodulcein protein, isfused to a signal peptide set forth in SEQ ID NO: 177. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 178. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 179. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 180. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 181. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 182. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 183. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 184. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 185. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 186. In some embodiments, the expressed Mycodulcein protein, isfused to a signal peptide set forth in SEQ ID NO: 187. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 188. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 189. In some embodiments, the expressed Mycodulceinprotein, is fused to a signal peptide set forth in SEQ ID NO: 190. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 191. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 192. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 193. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 194. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 195. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 196. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 197. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 198. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 199. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 200. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 201. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 202. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 203. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 204. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 205. In some embodiments, the expressed Mycodulcein protein, is fused to a signal peptide set forth in SEQ ID NO: 206.

[0085] In some embodiments, the coding sequence of a Mycodulcein protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 65. In some embodiments, the coding sequence of a Mycodulcein protein, is fused in frame to one or more coding sequence(s) of a signal peptide set forth in any one or more of SEQ ID NOS: 66-206 and is operably linked to a promoter set forth in any of SEQ ID NOS: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, or 65.Table 1. Exemplary Sweet Polypeptide Sequences.SEQ ID Peptide SequenceNO: Description214 Brazzein-54 XDKCKKVYENYPVSKCQLANQCNYDCKLDKHARSGECFYDEKRNLQCICDYCEY215 Brazzein-53 DKCKKVYENYPVSKCQLANQCNYDCKLDKHARSGECFYDEKRNLQCICDYCEYSEQID Peptide SequenceNO: Description216 Monellin FREIKGYEYQLYVYASDKLFRADISEDYKTRGRKLLRFNGPVPPPchain A217 Monellin GEWEIIDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPCMKKTIYEEN chain B229 Single-chain GEWEIIDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPCMKKTIYEENGFREIKGY monellin EYQLYVYASDKLFRADISEDYKTRGRKLLRFNGPVPPP218 Miraculin DSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRWQTRKE VDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQY FVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKG RRRLALSDKPFAFEFNKTVYF219 Mabinlin-1 EP LCRRQFQQHQH LRACQRYI RRRAQRGG LVDchain A220 Mabinlin-1 EQRGPALRLCCNQLRQVNKPCVCPVLRQAAHQQLYQGQIEGPRQVRQLFRAARN chain B LPNICKIPAVGRCQFTRW221 Mabinlin-2 QLWRCQRQFLQHQRLRACQRFIHRRAQFGGQPDchain A222 Mabinlin-2 QPRRPALRQCCNQLRQVDRPCVCPVLRQAAQQVLQRQIIQGPQQLRRLFDAARN chain B LPNICNIPNIGACPFRAW223 Mabinlin-3 EPLCRRQFQQHQHLRACQRYLRRRAQRGGLADchain A224 Mabinlin-3 EQRGPALRLCCNQLRQVNKPCVCPVLRQAAHQQLYQGQIEGPRQVRRLFRAARN chain B / LPNICKIPAVGRCQFTRWMabinlin-4chain B225 Mabinlin-4 EP LCRRQFQQHQH LRACQRYLRRRAQRGchain A226 Curculin 1 DNVLLSGQTLHADHSLQAGAYTLTIQNKCNLVKYQNGRQIWASNTDRRGSGCRLT LLSDGNLVIYDHNNNDVWGSACWGDNGKYALVLQKDGRFVIYGPVLWSLGPNGC RRVNG227 Curculin 2 DSVLLSGQTLYAGHSLTSGSYTLTIQNNCNLVKYQHGRQIWASDTDGQGSQCRLTL RSDGNLIIYDDNNMVVWGSDCWGNNGTYALVLQQDGLFVIYGPVLWPLGLNGC RSLN212 Thaumatin 1 ATFEIVNRCSYTVWAAASKGDAALDAGGRQLNSGESWTINVEPGTNGGKIWART DCYFDDSGSGICKTGDCGGLLRCKRFGRPPTTLAEFSLNQYGKDYIDISNIKGFNVP MDFSPTTRGCRGVRCAADIVGQCPAKLKAPGGGCNDACTVFQTSEYCCTTGKCGP TEYSRFFKRLCPDAFSYVLDKPTTVTCPGSSNYRVTFCPTA213 Thaumatin II ATFEIVNRCSYTVWAAASKGDAALDAGGRQLNSGESWTINVEPGTKGGKIWART DCYFDDSGRGICRTGDCGGLLQCKRFGRPPTTLAEFSLNQYGKDYIDISNIKGFNVP MDFSPTTRGCRGVRCAADIVGQCPAKLKAPGGGCNDACTVFQTSEYCCTTGKCGP TEYSRFFKRLCPDAFSYVLDKPTTVTCPGSSNYRVTFCPTA228 Mycodulcein MPDLSSFITIKNNSNHVFTRTAIYSKYAAVQWSPEPQLSISPGKWDLFILKDILSIRGTSGYVQYRVGDGPGWVRVTFSSLVGADEVAEWSSGDLPDGFVLQKPVRTGSRPLQATFEATKQSEQ ID Peptide SequenceNO: DescriptionX can be glutamine or pyroglutamate.III. Methods of Producing Sweet PolypeptidesExpression of Recombinant Heterologous Proteins (e.g., Sweet Proteins)

[0086] Provided herein are methods of expressing heterologous proteins (e.g., any of the sweet polypeptides described herein) using engineered promoters, engineered signal peptides, expression cassettes, vectors and / or host cells described herein. In some embodiments, the provided methods include expression, such as overexpression or increased expression, of one or more endogenous or exogenous transcriptions factors (e.g., a transcription factor with an amino acid sequence set forth in SEQ ID NO: 240 or SEQ ID NO: 241). The heterologous proteins (e.g., sweet proteins) can be produced recombinantly using a variety of protein expression systems, including, but not limited, cell-based expression systems, and cell-free expression systems. Heterologous proteins (e.g., sweet polypeptides) produced recombinantly are recombinant proteins.

[0087] A recombinant nucleic acid is a sequence that is not naturally occurring or has a sequence that is made by an artificial combination of two or more otherwise separated segments of nucleic acid sequence. This artificial combination is often accomplished by chemical synthesis or by the artificial manipulation of isolated segments of nucleic acids (e.g., by genetic engineering techniques). The term recombinant nucleic acids include nucleic acids that have been altered solely by addition, substitution, or deletion of a portion of the nucleic acid. Frequently, a recombinant nucleic acid can include a nucleic acid sequence operably linked to a promoter sequence. Such a recombinant nucleic acid can be part of a vector that is used, for example, to transform a cell.

[0088] Non-limiting examples of protein expression systems useful in producing sweet polypeptides disclosed herein include eukaryotic cell-based expression systems (e.g., filamentous or non-filamentous fungal cells, yeast cells, insect cells, and mammalian cells). In particular cases, a recombinant sweet protein is produced by a yeast cell.

[0089] To express a heterologous protein (e.g., a sweet protein) in a cell-based expression system, a gene encoding the heterologous protein can be introduced into a host cell of the expression system by, for example, a vector, whereby the vector further contains a promoter heterologous to the protein (e.g.,sweet protein). The vector can also encode a signal peptide whereby the signal peptide is associated with the heterologous protein to facilitate secretion of the heterologous protein (e.g., sweet protein).

[0090] A polynucleotide gene encoding the sweet polypeptide with or without a signal peptide, such as a signal peptide described herein, can be made by various methods, including molecular cloning and synthesis. Molecular cloning methods can involve mutagenesis (e.g., site-directed mutagenesis), restriction enzyme-mediated cloning (e.g., restriction enzyme digestion and ligation), polymerase chain reaction (PCR), and overlap extension. Synthesis can include chemical synthesis (e.g., gene synthesis). The gene sequence can be codon optimized for any desired expression system.

[0091] The coding sequence can be inserted into a vector by a variety of procedures, including, but not limited to restriction enzyme digestion, ligation, and homologous recombination. The vector can be capable of replicating and expressing the polynucleotides in prokaryotic and / or eukaryotic host cells of expression systems. A vector can contain various components that can be adjusted and optimized for compatibility with the particular host cell. A cloning vector and / or expression vector can include additional nucleic acid sequences, including but not limited to, a nucleic acid encoding a signal peptide (also called a signal sequence or signal peptide sequence), an origin of replication, a marker gene (e.g., a selection marker such as an antibiotic resistance gene), an enhancer element, a promoter, such as any promoter described herein, a ribosome binding site,. and a transcription termination sequence.Recovery and Purification of Sweet Polypeptides

[0092] Any heterologous protein (e.g., any sweet polypeptide), can be expressed using an expression system that employs any one or more of the engineered promoters, engineered signal peptides, expression cassettes, vectors, or host cells provided herein, and recovered, isolated, and / or purified. In preferred cases, the heterologous protein (e.g., sweet protein) can be recovered, isolated and / or purified from the supernatant of a cell culture, for example, if the expressed protein is secreted by the host cell into the cell culture medium. In some cases, the heterologous protein (e.g., sweet polypeptide) can be recovered from the host cell, for example, if the protein is expressed intracellularly if the protein is not secreted or not sufficiently secreted. Proteins can be recovered from the intracellular space by disrupting the host cell, for example, by osmotic shock, sonication, or lysis. Once the cells are disrupted, cell debris can be removed by centrifugation or filtration, and the expressed proteins can be removed or recovered from the cell lysate.

[0093] The expressed heterologous protein (e.g., sweet polypeptide) can be recovered and / or isolated from supernatants and lysates by any of a variety of methods, including, but not limited to, chemical extraction, column chromatography, and filtration. In some cases, the culture medium is collected after culturing the host cell. The polypeptides can be purified using any of a variety of methods including liquid chromatography such as normal or reversed phase, high-performance liquid chromatography (HPLC), fast protein liquid chromatography (FPLC), and the like; affinity chromatography such as with inorganic ligands, monoclonal antibodies (e.g., immunoaffinity), and ion exchange (e.g., anion exchange, cation exchange); hydrophobic interaction chromatography; size exclusion chromatography; immobilized metal chelate chromatography; gel electrophoresis; ethanol precipitation; and any combination thereof. In some cases, the polypeptides can be purified by centrifugation and / orfiltration, including sterile filtration, depth filtration, tangential flow filtration, ultrafiltration (UF), diafiltration (DF), and ultrafiltration / diafiltration (UF / DF).

[0094] A recovered and / or isolated heterologous protein (e.g., sweet protein) can be concentrated, dehydrated, dewatered, or dried by freeze drying, vacuum tray drying, spray drying, rotary drum drying, or any combination thereof. Drying can be done in a desiccator, vacuum dryer, conical dryer, spray dryer, fluid bed or any method known in the art. Preferably, methods are chosen that yield a dried product (e.g., a powder) with preserved sweetness. The resulting concentrated liquid or solid powder can be stored, diluted, or rehydrated. In some cases, the concentrated liquid or solid powder can be stored at -20°C, 4°C, or room temperature without significant loss in sweetness. In some cases, the concentrated liquid can be diluted using water or a fat. In some cases, the solid powder can be rehydrated at a paste or a liquid using water or another liquid in which the solid powder is soluble.

[0095] Although preferred for use in some cases, there is no general requirement that the protein, polypeptide, or peptide always be provided in their most purified state. Indeed, it is contemplated that less substantially purified protein, polypeptide, or peptide, which are nonetheless enriched in the desired peptide compositions, relative to the natural state, will have utility in some cases. Methods of enrichment of a component, such as enrichment of a peptide, are known in the art and are often similar to those of purification of components or peptides.

[0096] In other cases, a preparation enriched with any of the heterologous proteins (e.g., sweet peptides) can be used instead of a purified preparation. In this document, whenever purified is used, enriched can be used also. A preparation can be enriched not only by methods of purification, but also by the overexpression or over-production of the peptide by the host cell when compared to a wild-type host cell. Thiscan be accomplished using recombinant methods, or by selecting conditions which will induce the expression of the peptide from the wild-type cells.IV. Definitions

[0097] Reference to the term "about" has its usual meaning in the context of compositions to allow for reasonable variations in amounts that can achieve the same effect and refers herein to a value of plus or minus 10% of the provided value. For example, "about 20" means or includes amounts from 18 to and including 22.

[0098] Unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. As used herein, the singular form "a," "an," and "the" include plural references unless indicated otherwise. For example, "an" excipient includes one or more excipients.

[0099] The term "operably linked" indicates that the linked nucleic acid (e.g., a gene of interest) and the regulatory sequence(s) (e.g., a promoter) are connected in such a way as to permit transcription, thus producing RNA from the linked nucleic acid sequence (e.g., transcribing the gene of interest). In some cases, the activity of a promoter can be measured directly, such as by assessing the level of RNA produced.

[0100] As used herein, the term "amino acid" refers to either natural and / or unnatural or synthetic amino acids, including pyroglutamate (pGlu) and both the D or L optical isomers, amino acid analogs, and / or peptidomimetics.

[0101] As used herein "recombinant protein" is a protein produced from a recombinant nucleic acid.

[0102] The term "purifying" means a step performed to isolate a recombinant peptide from one or more other impurities (e.g., bulk impurities) or components present in a fluid containing a recombinant peptide (e.g., liquid culture medium polypeptides or one or more other components (e.g., DNA, RNA, other polypeptides, endotoxins, viruses, etc.) present in or secreted from a cell, such as a filamentous fungal cell).

[0103] As used herein "enriched" refers to a component, such as a peptide or protein that is found at a high concentration in a solution or composition than in nature when produced the same way.

[0104] The terms "isolated", "purified", "separated", and "recovered" as used herein refer to a material (e.g., a polypeptide, nucleic acid, or cell) that is removed from at least one component with which it is naturally associated, for example, at a concentration of at least 90% by weight, or at least 95% byweight, or at least 98% by weight of the sample in which it is contained. For example, these terms can refer to a material which is substantially or essentially free from components which normally accompany it as found in its native state, such as, for example, an intact biological system, or is substantially or essentially free from other proteins in the system from which it is expressed.

[0105] Where the term "substantially purified" is used, this will refer to a composition in which the specific protein, peptide, or peptide forms the major component of the composition, such as constituting about 50% of the peptides in the composition or more. In preferred cases, a substantially purified peptide will constitute more than 60%, 70%, 80%, 90%, 95%, 99% or even more of the peptides in the composition.

[0106] A peptide, polypeptide or protein that is "purified to homogeneity," as used herein, means that the peptide, polypeptide, or protein has a level of purity where the peptide, polypeptide or protein is substantially purified or free from other proteins / peptides and biological components. For example, a purified peptide, polypeptide, or protein will often be sufficiently free of other protein / peptide components so that degradative sequencing can be performed successfully.Table 2. Exemplary Sequences.Sequence SEQDescription! DNO. SequencePl 1 AGAGATTCTCCGTCCAACTTTGACAGAGGTATTGAATAGTCATTTTCTATTCCCGATTACCCTATTGGTGTTCGC GCTTCTCTCTAAGGTGACGCAATAAGAATGTGGATCGACGATAGCATCAGGAAAGATCGACATCCACGATTCT TGAAAAATATACTGAGGGAGAAAATAAAGTTCACACGTACAATCCTAGCGCACCCCTTAAGGTCCCTCTAGCC CGTGTCCTCTCGAAACGTTACTCCGGAGTCCTTACAATACCAAGTAATTGGGGGTGTGACCTAAACGTCTCCT AGAATCCGGGGCAACACCCTAGAGGCTCATTTCAAAATGAGATCCACTAACGCGGTATCTTAGCCCGTAATCC ACAATAGCCCTGGTACCTGACTCGGTGTGCGTCTGCTATTTTACCACCCAAGTTACTACCGCCAACCACAGTCT TTCAAGTTCAAATCGGACCCGTTTTTTTGTGAGGCACTTTGACGGGGGTGTCGAGATTGTTTTCAATCGCGAA ACCGTGACCTTCCACCCTCGCGAAAGGAGCTCGCTTCCGAGAAGTTCATGACGATTTCACCATCAAAAGGCAC ACATTAAACAATAGTCCAAAAACACCGCTTATCGATCTCGGAATGGTTTCGGATCTTCAGCATCGTTTAAAAAA CTGCCAACCTACCCAATTGCGAGCTCAATGGACC 1 1 1 1 CCGTGTATTCCGGTGGTTATTTACCAACATTCCAAT ATCGATGATACTCGCCAAATTGGAATCTTTOTGTGTGGCGCACGTCTTTCACCCCAGCTTTTCCATACAAGGCA GTTCGAATATCCCTCCGGCGGTGCAACGGGCAGTCGTTGCATCCTCGTTCTCAAATATCTCCATATCCTAATTC ATGCCCTATAATTCATATATATAGGGCTCTTTCGTCATCTTTTGTCGAATTCTCTTTTTTTCCTCTCCCCCTTCTTT TAATTATCAGTAATCCTCCTCGGG 1 1 1 1 1 1 1 i AAAAGTATTGTCAAACP2 2 ATCGAAAAGAAGAAGATCCAGAAGGCTAGCCAAAGAAAAAGGACGAGACTTCGAAGACCACCACCAGATTC CGGGGAAGCAAACAGAAGAACAAAAATTAGTGAGAATGCTGTTATCAACTGAAAGAAACAACAAGAGGAGG AATGGAAGAATAGTTCTTTGTCTGGAAGACTTTACGAAGTTCATATTAGAATCGGTGAATGACTCCCATGCCA TCATACAGCGTTGTCTAGTTGTGCGGGACACCCAAGATGACGAAGAGATCAATAAATGGATCGGAAATATTT CCACGAAGATCACCATTTTGGAAACAAACAGTTGAAAAACACCATATAGGAGTAATAGTCTCTAGTAATAATT ATACCCTCAACCTTTGGCCCTCATACTTCGAGTTTTCATTTAAATTAACTGAGACAAGAAAAGACAAAAACGTG CCATCATCTCTCTCTTTTGAATTAGTTTAGCGCTGTTTCAATCAAGTTAGATGTTCTCAACTACCAATAGTCTATAACAATTTCAAAAAGAGAACCCTACTTTTCATTGCTCTTACATCATAAAGAAAAAAAATAAACATACTGAACCTSequence SEQDescription iDNO. Sequence TTTAACACTCCGATGGCAAGTTCATCAACTAAAATGCTATTATTCAATCGCTAAGCTACTATATATATATATTTT GACGCTCCATCGATGTGGTGATTGTTGTCCCATCTCTTGTAAGCACTGGATCAGGTACCTTAACAGTAGGTTCC CCATGAAAAACGATATGTACGACATCACAATAGTTGTGGGGTCGTGCl!! IACCGTAGATCGGGTGTCAGTTT CGTGTTAACCAATAAAAAGAGTCCATCCTCGACAAAAAAGGTGGCCTCGGTGTTATGTGCCCGGACACTATTT TTCACTCGATGCTCCTTACTCCCTTGCCAATTAGCGCTCGCTCTTATTTGACAACACCTTTCCTTCTCTCTTTTTCT TCTTCTTACACCAGAATAAGTGATTTAATTATTCGGATCATTACTAGCTP3 3 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTG CTTTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTTmGCATTTGCCACTTATCTAAAAAT ACI 1 1 1 GTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTAI I 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCAGGTGAACCCACCT AACTA! 1 I 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTTCGGGGAACCGTGCT CGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCGTTTTCATCGTAGCGT GGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 1 1 1 IAACCTTAAA GTCGTTCATCAATCATTAACTGACCAATCAGAI I l l i I GCAI 1 I GCCAC I 1 A 1 C 1 AAAAA 1 AC 1 I 1 I G I ATCTCGC AGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTATTTT TGGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAGCT TCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAA GATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCCTTTTCGTCGTCGAGCCTGCTTCATTCCTG CCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACA AATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCC CCCCTGGTTCTCTTrfTCTTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAAAA P4 4 CAGGTGAACCCACCTAACTATrfTTAACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAAI 1 1 1 1 I I 1 1 CCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTG CTTTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTTmGCATTTGCCACTTATCTAAAAAT ACI I 1 I GTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTAI I 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGG 1 I I G I 1 IA IAGCI 1 1 1 CGCCTGGTGCCGTACGGTACATACT CTCCTCCCCCCCCTGGTTCTCTTTTTCTTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAA AACAGGTGAACCCACCTAACTATTTTTAACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCI I 1 I G TTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAAI 1 I I I I I I 1 CCCGCGCAGCTTTAATCTTTCGGCAGAGAAGG CGTTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTT TGCTTTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTTTTTGCATTTGCCACTTATCTAAAA ATACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAG GCAGTCGGTTTTA 1 1 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGT AACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAA ACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCG AGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAAASequence SEQDescription iDNO. Sequence TACATACTCTCCTCCCCCCCCTGGTTCTCTnTTCTTrTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTC AACAACAAAAP5 5 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 1 1 I I AACC 1 1 AAAG 1 CG 1 I CA I CAA 1 GA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAAT ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTA! 1 i 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATACAGGTGAACCCACCTAACTA! 1 I 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAA AGCCAACCATCTTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATC TTTCGGCAGAGAAGGCGTTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATG GCAGCAGTCACTATTTTGC 1 1 1 1 1 AACC 1 1 AAAG 1 CG I 1 CA 1 CAA 1 CA 1 1 AAC 1 GACCAA I CAGA 1 1 1 1 1 1 GCAT TTGCCACTTATCTAAAAATACI 1 1 1 GTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAG GTATCAATGCCACTAGGCAGTCGGTTTTAI 1 1 1 I GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTT AAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGAT GCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTC CAGCCI 1 1 I CGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGG CAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTG GTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGI I CI CI 1 1 1 1 CTTTTGTTACTTACATTTTACCGTT CCATCACTCGCTTCACTCAACAACAAAA PS 6 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 1 1 I 1 AACC 1 1 AAAG 1 CG 1 I CA I CAA I CA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAAT ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTA 1 1 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTCAGGTGAACCCACCTAACTA I I 1 1 1 AACTGGGATC CAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTT1 I I 1 1 CCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCGTTTTCATCGTAGCGTGGGAACAGAATAATCAGTT CATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGCTTTTTAACCTTAAAGTCGTTCATCAATCATTAACT GACCAATCAGA I I I I 1 1 GCA 1 1 1 GCCAC I I A 1 C 1 AAAAA 1 AC 1 1 1 1 G 1 ATCTCGCAGATACGTTCAGTGGTTTCC AGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTA 1 1 1 1 1 GGTCACCCACGCAAAGAAG CACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTG TGACCGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAA TCGATCCATAGTTACCCATTCCAGCC 1 1 I 1 CGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGC ATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGT TTGTTTATAGC I l l i CGCC I GG 1 GCCG I ACGG 1 A 1 AAA I ACA I AC 1 C 1 CC 1 CCCCCCCC 1 GG I 1 C 1 C I 1 1 1 1 CTTT TGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAAAAP7 7 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCGTTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGSequence SEQDescription iDNO. Sequence CTTTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTHTreCATTTGCCACTTATCTAAAAAT ACI I I I GTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTA i 1 i 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGG 1 i I G I 1 IA IAGCI 1 1 1 CGCCTGGTGCCGTACGGTATAAATA CATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 CTTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAA CAACAAAAP8 8 CAGGTGAACCCACCTAACTATTTTTAACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAAI 1 1 1 1 1 I I 1 CCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTG CTTTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTnTTGCATTTGCCACTTATCTAAAAAT ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTATTTTTGGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCI 1 1 1 CGCCTGGTGCCGTACGGTATAAATA CATACCAGGTGAACCCACCTAACTA 1 1 I! 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTT TTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGA AGGCGTTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTA TTTTGC 1 1 1 1 1 AACC 1 1 AAAG 1 CG 1 I CA I CAA 1 CA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTA AAAATACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCAC TAGGCAGTCGGTTTTA 1 1 1 1 1 GG 1 CACCCACGCAAAGAAGCACCCACL i LI 1 I I AGGTTTTAAGTTGTGGGAAC AGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATT TAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCCTTTTCGTCG TCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTT AAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTAT AAATACATACTCTCCTCCCCCCCCTGGTTCTCTTTTTCTTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCA CTCAACAACAAAAP9 9 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 1 I 1 I AACC 1 1 AAAG 1 CG I 1 CA 1 CAA 1 CA 1 1 AAC 1 GACCAA I CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAAT ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTA 1 i 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAAATA CATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 CTTTTGTTACTTACATTTTACCGTTCCGTCACTCGCTTCACTCAA CAACAAAA PIO 10 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGCl 1 I I 1 AACC 1 1 AAAG 1 CG 1 i CA 1 CAA 1 CA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAATSequence SEQDescription iDNO. Sequence ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTAI I 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC 1 i 1 1 CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAAATA CATACTCTCCTCCCCCCCCTGGTTCCAGGTGAACCCACCTAACTAI 1 1 i 1 AACTGGGATCCAGTGAGCTCGCTG GGTGAAAGCCAACCATCTTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGC TTTAATCTTTCGGCAGAGAAGGCG I I I I CATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGG CACATGGCAGCAGTCACTATTTTGC 1 1 I 1 I AACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTTT TTGCATTTGCCACTTATCTAAAAATACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAA AAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTATTTTTGGTCACCCACGCAAAGAAGCACCCACCTCTTTTA GGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACC ATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTAC CCATTCCAGCCTTTTCGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGA TTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTT CGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 CTTTTGTTACTTACATTTT ACCGTTCCATCACTCGCTTCACTCAACAACAAAAPll 11 CAGGTGAACCCACCTAACTATHTf AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 1 I 1 1 AALL 1 1 AAAG 1 LG I 1 LA 1 CAA 1 LA 1 1 AAL 1 GALCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAAT ACI 1 1 I GTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTAI 1 I 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCC I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCI 1 1 I CGCCTGGTGCCGTACGGTATAAATA CATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 C I I 1 I G I I AC 1 1 ACAGG I GAACCCACC I AAC I A 1 1 1 1 1 AACTGG GATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAA TTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCGTTTTCATCGTAGCGTGGGAACAGAATAATCA GTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC H i l l AACCTTAAAGTCGTTCATCAATCATTA ACTGACCAATCAGA 1 1 I I 1 1 GCA 1 1 1 GLLAL I I A 1 L 1 AAAAA 1 AL I I 1 1 G 1 ATCTCGCAGATACGTTCAGTGGTT TCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTAI 1 1 1 1 GGTCACCCACGCAAAG AAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTAC CTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAAT CAATCGATCCATAGTTACCCATTCCAGCCTTTTCGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTT TGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAA GGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 C TTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAAAAP12 12 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 I I I I AACC 1 1 AAAG 1 CG I I CA I CAA I CA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAAT ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTAI 1 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCL I l l i CGTCGTCGAGSequence SEQDescription iDNO. Sequence CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCI 1 1 I CGCCTGGTGCCGTACGGTATAAATA CATACTCTCCTCCCCCCCCTGGTTCTC H i l l CTTTTGTTACTTACATTTTACCGTTCCATCACTCAGGTGAACCCA CCTAACTA I 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTTCGGGGAACCGT GCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCGTTTTCATCGTAG CGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGCTTTTTAACCTTA AAGTCGTTCATCAATCATTAACTGACCAATCAGAI I I I I I GCA I 1 1 GLLAL I I A 1 L 1 AAAAA 1 AL 1 1 1 I GTATCTC GCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTATT TTTGGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAG CTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATC AAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCCTTTTCGTCGTCGAGCCTGCTTCATTCC TGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAA CAAATATACCGCGAAAAAGGTTTGTTTATAGLl 1 I 1 CGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCC CCCCCCTGGTTCTCTTlTrcnTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAAAA P13 13 CAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCATCTTTTGTTT CGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG TTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCACTATTTTGC 1 1 i 1 i AACC 1 1 AAAG 1 CG 1 1 CA 1 CAA I CA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTATCTAAAAAT ACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGCCACTAGGC AGTCGGTTTTA I 1 I 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGGAACAGTAA CACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAAC GAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCL I l l i CGTCGTCGAG CCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAAT AGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCI 1 1 1 CGCCTGGTGCCGTACGGTATAAATA CATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 I LI I 1 I G I 1 ACTTACATTTTACCGTTCCATCACTCGCTTCACTCAA CAACAAAACAGGTGAACCCACCTAACTAI 1 1 1 1 AACTGGGATCCAGTGAGCTCGCTGGGTGAAAGCCAACCAT CTTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAATTTTTTTTTCCCGCGCAGCTTTAATCTTTCGGCAGA GAAGGCGTTTTCATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGCACATGGCAGCAGTCA CTATTTTGC 1 1 1 1 1 AACC 1 1 AAAG 1 CG 1 I CA 1 CAA 1 CA 1 1 AAC 1 GACCAA 1 CAGA 1 1 1 1 1 1 GCATTTGCCACTTAT CTAAAAATACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAAAAAAAGGTATCAATGC CACTAGGCAGTCGGTTTTA 1 1 1 1 1 GGTCACCCACGCAAAGAAGCACCCACCTCTTTTAGGTTTTAAGTTGTGGG AACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTA ATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCCI 1 I I CG TCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGA GTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACG GTATAAATACATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 CTTTTGTTACTTACATTTTACCGTTCCATCACTCGC TTCACTCAACAACAAAAP14 14 CATATTCAAATACCAAGCAAATAAACGCAAAGAGCAACATATTAGGATAAACCAACAGGTTAAGTATAGAGC TAAAGCATTGCTGAGCAATATTTCGGATCAACATCAACAGAATAGATCGTCACCACGAAGTATATCCAACTAT TCCCCAAGAATCCAGGCTTATCCCTCAAGAATGGCCTCTCCATCTCCTTCAATGAAGATGGCATTGACAAACTC CGTCAGCTTGCAGGATACAATGGATGCGGAACTAGAAGCGTTGGCAAACGAACAATATTACGTCATGCTAGA TATCCTTAAGGGATTTTGCGATCTTTCATTTGACATGGTAAACATATTTTCTATCCAGATCCCTGAGGTATTACA TCTCCTCTTCGGCTTTGGTGCAGGGTCACTGGCCTTAACGGGAGTCGTATCCAAAACGAGACAAGAACTAATA AATAAACAGATATAAGGGACAAGCACACGATTACCCAATCACTTGATATGCACCAATTTGTTCCGTTGTTTATG CCATATTTACCGAATTTTCTTCCCAGGTTTTTCCGAATGGACATCTGTAGTCCACTTTTTGGTTATCATAATCGTC CCACAAGTCGTGGATTTAACCAGAACCTAGTAATTTTAAGTTCGCTATTAATCACTCAGAATGGTCTCACCTTG CTATTGGCCAAGTCTGGAGTCGCCAGCTACCACCTCAGAGGCTACATAGACCTCCCAATGTCATCTCCTCAGTGCGCTCTTCAATCTCGTGTCTTTTCCGTTAAAACTCCGTTCGTTTCACCCTATACTGCCCCTGGTTGTGCAGCTCSequence SEQDescription iDNO. Sequence TTACCACTTCGCGCCGCTACTATCCGTAGTGGTCGAGCCGCATCAATATCACGTTGAAATAGAATAACTCCCTA CAAAAGCCGCACGCAACCATCAAATCTATATAAGGAACCTCAAATATCTAGCAACATCTTTTCAATTTACTACA ACATATTCGTTAATCATCAATCAATTAGCTAGTACACAACAP15 15 CTACATCGGAACCAATTTTGAATTCGCCGATACGAAACCGGACATCAAGTTCCATCATCATGCAAGATGATCA TCAGGAAGAGCCGTGGTCAGTCAACACTGCAAGTGTCAAAGAGACATCAATTATTGACATTAGTACCCCGGA TGAGGCTTACAATGCTCGCGGGAAAGACGATGCGATACGGGTTGATTCGGAGGGCCCTTCAAAAAGAACAA CTATTCTCAGCCGTCTGATGGAGACACAAGATTCGGACAATGACGGAACGGAGTCTGACGTAGGGGAGAGTT CTAGTCTGATAATTCTTAGTTGACCAGCCTTGCATCGGGTTGAACAAACTTTTGTGGGTTGGGCCACTTTGCGC CAACAACACAAGGTTTTCACACCACCCGGTTGTCATTACCGCCCGGAAAACTCAATGATTGACGGAGCGTAGG TTCACGGAAGTCAAGTTAGAGTAGTCGGAGAGTTTCGGGTGATAAGAATTTCCCGGGTGCTTTGGAGTGCCA AAGTGACTGAATCAGGAACTAAAAACCCGGGCTAACGGCTGAAGGCCTGTGTTCAGTGCACTATATTGTATTC TAGTCTGGGGTAGGTTGAAGTTTGGTGGATTCACGACTCCGGAGTGATGGAGGGACAATACCGAAGATGAG GTCTTGCGAGTGGAAAGATAGGTTAAGAATAATTAATAACATTGTATGAAACAAGATGGGGAGACAATTTTA GCTATCGATCCGTTATCATTGGTCGAATGATGACTTGGAACCTTATTTGCACTTTTTTTCTCAGGTGTTCGCCG AAACTTAAAGATAGTTTTATTGATGTGTGCCAAAAATGTGGGGACAAAAGGTCCTCCACCCGCCATCCCCCGG TCTCATACAAAAAAAAAGAAAAATACCTCTACTAGTCCGGGGTCAAGGCAAGTCATGATAGGCATATAAATA GGGGCAGGCTGGCCGCCATTACGAATTAGATTAACCTTCTCATAGATTATTATCCACAP16 16 GAAGGTGGAAATGGCAGAAGGATCAGCCTGGACGAAGCAACCAGTTCCAACTGCTAAGTAAAGAAGATGCT AGACGAAGGAGACTTCAGAGGTGAAAAGTTTGCAAGAAGAGAGCTGCGGGAAATAAATTTTCAATTTAAGG ACTTGAGTGCGTCCATATTCGTGTACGTGTCCAACTGTTTTCCATTACCTAAGAAAAACATAAAGATTAAAAAG ATAAACCCAATCGGGAAACTTTAGCGTGCCGTTTCGGATTCCGAAAAAC. I 1 1 1 GGAGCGCCAGATGACTATGG AAAGAGGAGTGTACCAAAATGGCAAGTCGGGGGCTACTCACCGGATAGCCAATACATTCTCTAGGAACCAGG GATGAATCCAGG 1 1 1 1 1 G 1 1 G i GAGGG 1 AGG i GAAGGA 1 1 GAG i 1 C i i AGGAATATCTCGTTGAAAGCTACTTG AAATCCCATTGGGTGCGGAACCAGCTTCTAATTAAATAGTTCGATGATGTTCTCTAAGTGGGACTCTACGGCT CAAACTTCTACACAGCATCATCTTAGTAGTCCCTTCCCAAAACACCATTCTAGGTTTCGGAACGTAACGAAACA ATGTTCCTCTCTTCACATTGGGCCGTTACTCTAGCCTTCCGAAGAACCAATAAAAGGGACCGGCTGAAACGGG TGTGGAAACTCCTGTCCAGTTTATGGCAAAGGCTACAGAAATCCCAATCTTGTCGGGATGTTGCTCCTCCCAA ACGCCATATTGTACTGCAGTTGGTGCGCATTTTAGGGAAAATTTACCCCAGATGTCCTGATTTTCGAGGGCTA CCCCCAACTCCCTGTGCTTATACTTAGTCTAATTCTATTCAGTGTGCTGACCTACACGTAATGATGTCGTAACCC AGTTAAATGGCCGAAAAACTATTTAAGTAAGTTTATTTCTCCTCCAGATGAGACTCTCCTTCTTTTCTCCGCTAG TTATCAAACTATAAACCTATTTTACCTCAAATACCTCCAACATCACCCACTTAAACAP17 17 GATAGTGTAAGTCAGGTGCATACTCACGTGAAAATCATGGAAAAGGCAGTCGATGTCGGAGGTACTGTTAAA AGGGAAGGAAACCTGCTGGAACACACTCTCATCCTCAATGGGAGCTGGAGTGAGGCGCAGCAGTCAATCGTC TGGCCATCGCCCAAAAGAAATACTCACATACCCACATAGAACCAGTCCAGAATACTGGGGTGTGTAGGTTCCA TTTCATTCTGACTCATCTTCGTCTTGTTGTCTCTTCCGAACATGCGAAGGAAATCTGTACATAAAAATCCTTACA TCAGATTAAGAACAACCTGCGATCACGGGTTGTGTCCCCGGAGTTATGGCGACTTAGGCGGTGAAATCTGGT GGATTGGAAAGAAACCTACGCACTATCGGGTACACAGGAGAATCGGGAAAATGTCACCTGCTTATCCTTACT ACAATTGCATGTGGGGTGATTCTGAAACCTTAGCATAATTTGTAGGCATTCTGGGAAAGATATTTTGCGCCGG CCTTACTAGTATTGTTGAAGTGACACCAAGCAAACCGCTCTAAAAAATCTGTGAACACTGCTTAGCCATCAACA CTAACACTTGGTGGTTATTCTATGACTCATTTAAAGTCGGGGTGTTGGGGTGATTGGAAGATTCCAGAATTAA CCAGGGCCGGGATG 1 G 1 i G 1 GGGGG 1 G 1 GAG 1 H 1 GA H G 1 1 1 G 1 G I 1 I 1 1 GGTGGGGGTGAGTTAGGACGTT CGGCAAAAAAATAAAATTCTACGCCAAATTGGGTAATTCGAGACCCTATCAGCCACCAAAACACAGCAATATG TGGGACCGATAGGGGGTGCAATCTGTTTCGCTAATGACGTACTTCCCGGGTGTCCAGATATCGAACACCCTCA CGCACATTCGGATGATGAGAAAATGAGCTAACCCCAGATTTCTTCGAATTTAGTACTTAAGAATACGATAACC CCCGCAATTGACCTTCCCTATTTTTACACATCTTATCACACCAAAATTACAAP18 18 GGGTGAAAGCCAACCATCTTTGTTTCGGGGAACCGTGCTCGCCCCGTAAAGTTAA 1 1 1 I I I I 1 i GGCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCG I I I I CATCGTAGCGTGGGAACAGAATAATCAGTTCATGTGCTATACAGGSequence SEQDescription iDNO. Sequence CACATGGCAGCAGTCACTATTTTGCTTTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACCAATCAGATTTT TTGCATTTGCCACTTATCTAAAAATACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGACAACACCCAA AAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTA 1 i! i IGGTCACCCACCCAAAGAAGCACCCACCTCTTTTA GGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGCAATTCACC ATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCCATAGTTAC CCATTCCAGCCTTTTCGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAAGTCCAGA TTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTT CGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 CTTTTGTTACTTACATTTT ACCGTTCCGTCACTCGCTTCACTCAACAACAAAAP19 19 GTAGTTGTTTTCAGCAGATCCAGGGAGGGCATCGTTGAGGTTTCCTCAAAGGGAAGAAACATGGATCCGGCG ACATCAACAGACAGAGAAGCGGGTAGTGAATCCGAAGCCACAACACAGCCCGATTTGGAAGGGAGCCCACC ATCAAAATAAGTTCCAGCCA I 1 1 1 1 I I ICI I 1 I ld’ICI 1 1 I I 1 1 1 1 1 1 1 GGTCAATTGAGTCAGACCCACCTAACT Al 1 1 1 1 AAAATGGATCCGGTAATCTCGCAAGGAAGAGACTAACCATCTTTCGTTTCGGGGAACCGTGTGCTCG CCCCATCTTGCTAATTTTTTTCCCGCGCAGCAGAAGAGGCGTTTTCATCGTATCGTGGTAACAGAATGGTCAGT TGATGTGCCGTACAGGCACATGGCAGCAGTCACTATTTATTTTTTTAACCTTAAAGTCGTCCATTAATCATTACC TGACCAATCAGATTTTCTGTATTTGCCACTTATCTAAAAATACTTTTCTATCTCGCAGATACGGTTCAATGGTTT CCAGGACAACGCCAGAAAAAAGAAGTATCAATGCCACCAGGCAAGTCGGTTTTATGTTTGGTAGTCCCACGC AAAGGAGCACCCACCTCTTTAAAGTTGTGGTTAACCACCTGAAGCTCCGGAATAAACTACTGGCTGTGACCAC AACAGTCCAATGATGCAGAATGCTAATTTAAACGAGTGCCAAATCAGGATTTTCACAGATAAATCAATCGATC CAGAGTTACCCATTCCAGCC 1 1 1 1 C 1 GGG 1 CGAGC I I GC 1 1 CA 1 I C I I I 1 C 1 CAGGTGCATACGTCTGCATGAAC AATCCAGATTAGGGCAGATTTTGAGTTTAAATTAGGAAATATAAACAAATATACCGCGAAACTGGTTTGTTTA TAGCTTTTCGCCCACTGCCGTATGGTATAAATACATACTCTTCTTCCCCCCTGGTTCTCTTCTTCTTTTGTTACTT ACATTTTACCGTTCCGTCACTCGCTTCACTCAACAACAAAAP20 20 GTTGGAGGGTGAGCTGAGCTCAGATTTGCTCCCAAGTGTTCTACAAGGTCAGCGTGGCCGTCATCGTTGTCGC TGTCACTTTCTACGACAGTCTCATCTAAGTAGAAAACCATATCTAAAGATAGTTAGACTGGAGCCAAGGCCAA TTGTGGGTTCTAGATTGATAAAAAAAACGAGACGATAAGATGAGGAAGGTACCACACATGGGCATTCTTAGT GCGCGAGAGATGATTAGCATCGAGGGAAAGCTTAAACATCTTTGGTCTACGTAAGCAGAGACCAGGCACTAG CAAGCCTAATTAGGGTTAGGGAATTGAATGTCAGCAAAAGCTGAGGCGGCTTCCGAGGGCCAATAGAATAA GAAAGAACAACTTAGGGCGCAAACCTGATTGCGATTTTGGGGCTTTCCTTGGAAAAGACTTGATCCCTACGCT GTGGAAGGCGCACTACTATCGAAGCTCCCTCTAACCTCCCAAAGGAGAAGGAAGGGAAAAAAAAATAGTGA CAAAAAGAAAACAAAGAGCCCAAGACCTCTATCGCCCCATCGCCCAGATCTCCTATCAGCAAAATTATGTAAG CTGCATCTTTTGGTGAGCTAAAGGGGACTTTCGCGCTAACAAAAAGAGCAAACTTGTTTGTTGGGTGATTGTT GGGTGTTCAAGGCACGACTTTCTAATCTACCTTGCATTGACAGATTCTTCCAACTGCGCCCGATATAACGTAGC ATTGCCAGGTAATGATGGTATACTTTACATGGTCACACTACGACGCTCAACATCAGTCCCTCTTAGTGGAACCA CAACTTGCTCGTTGAATTTTGGAGCGTAATGTGTCATGTTGGGTCCTGCAAAAAGAAAAGTTGGATCCCATAA ATTTAGACTTTGTAGGATGACAATCTACAGAGATTTCTCGAACTTCGGGCCTTCCTATAAAACAAGATAAACTC CTTCCTC 1 I I C 1 C I I 1 CL 1 1 L 1 CTTTAGTCTTCTCACTTCATCTACGCCACACAP21 21 TCTGCTACTCTGGTCCCAAGTGAACCACCTTTTGGACCCTATTGACCGGACCTTAACTTGCCAAACCTAAACGC TTAATGCCTCAGACGTTTTAATGCCTCTCAACACCTCCAAGGTTGCTTTCTTGAGCATGCCTACTAGGAACTTTA ACGAACTGTGGGGTTGCAGACAGTTTCAGGCGTGTCCCGACCAATATGGCCTACTAGACTCTCTGAAAAATCA CAGTTTTCCAGTAGTTCCGATCAAATTACCATCGAAATGGTCCCATAAACGGACATTTGACATCCGTTCCTGAA TTATAGTCTTCCACCGTGGATCATGGTGTTCCTTTTTTTCCCAAAGAATATCAGCATCCCTTAACTACGTTAGGT CAGTGATGACAATGGACCAAATTGTTGCAAGGTTTTTCTTTTTCTTTCATCGGCACATTTCAGCCTCACATGCG ACTATTATCGATCAATGAAATCCATCAAGATTGAAATCTTAAAATTGCCCCTTTCACTTGACAGGATCCTTTTTT GTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAAC GACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCSequence SEQDescription iDNO. Sequence TTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGG GATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACC AGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTAT TTGTCCCTATTTCAATCAATTGAACAACTATCAAAACACAP22 22 TCTTATTCTCAACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGA CTGCCCAACCTTAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCG GAGATGTATTGACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACG GGAGTGCCTGTTCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAAT TTCGTTGATTCCCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGT CGACTACATAAAGCGACCGGTGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGA AAACGGGGGTGAGCGCTCTCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAA CCCGCATCTGGTGCGAATATAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAA TATTCACAATTCACCTCACTATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGC 1 1 I I ATCTTATTTACTTTACGAAAP23 23 TGACATTCCTCAGTGTGGCTAATGGTACTTACACATTCACTGTATTCGAGTACCAGTCAGCTTTGATTGCCCGA TTCCTCACAGGTAAGGTAGAATTACCAGAAAGAGAGGAACAAGAGGCTTGGATTCAGAACAGAGTCAATCTG AAGTCTGACACCACC 1 1 1 1 1 CCACATGATTCCATACGAAGAGGCTGGTGAATATTTCCAGGACTTGGTCAGGC TGGCTGCTGATCCTGAATTGACTTTTGACAGCGACAAAATCTACAACGCTACCAAAGCAGGCCAAGAACTCAA GTCGAAATACTGGGCCCGTAACAAAGAGATCAGAAAATTGGGAACCAGTGCTTAATCCATACGTCGCTGGCA TTATTAAACTAGGTTTTGATGAAAACTCAGCATGTGATTGAGAACTCTCAGTTGGTTCTTTTTCGTTTTCACTTT ACGAATTGTTTTAGAAAGGCATCATCTAATGTAATCTGTATACACTTTATTGCATTATATATACTCCACCTTGCT GTTCATCTCGTCATCTTCGGATTCTTAGCTGCTCCATCTGCCCCGGGGGTGTGAAATGTCCGACTCTCCGAGCA GACGGTTGAGCCTCGCGCCGCATCAACGGATAAGGCATGGTAGTGACCCCTCTCAACAGCGGGCCATACAGT TCTCCGCCCACCGTCGCCGCAAGGATCACCATAAAACCTTGCTGCTCCCGGCGTTCTTGGGTACTTGACGGAC GGAGAACAGACAGGTTTCAGACCCCCCCGGAAGTTGACACCTAGGTCAGTTATAATTGCAAGTCACACTACCT GCACATAGTAAATTGCTCTCACCCGGTTGAGATCCGACCAGTTCTGTTGACTCTGTTTGCTTCCATGACTCTGC TCCCCTTGGCCGACTGATAAGCATGTTCATCCCATCGGCCATTGCCGTGACTCGGGAATGACACCCCAGCAAA CGTATATAAACCCACAATCCCCCCAGATTTACCGCTTTATCACAAAAP24 24 TGTTTATGCCATCGTGAACACAAATATCACAGGTGCATTTGGTGCTATTTCCTGGTGTCTATTAGACTGGCGTT TGGAGCGCCGTTTCAGTACTGTTGCTCTATGCTCCGGTGCCATTTCGGGCCTCGTGGCAGCAACTCCAGCCTC AGGTATCATTCCTCTTTGGGCCAGTGTTATTCTTGGTATTGTATCAGGAGTGGTTTGTAACTACGCAACCAAGA TTAAAGTCATTTGTCGAGTCGATGATTCCATGGATGTTCTAGCAGAGCACGGTATCGCTGGTGTTATTGGTCTCG 1 C I 1 CAACGCATTATTTGGGTCGGCTACTGTCATTGGTTATGATGGCCTTACCGAGCACGAAGGTGGTTGGA TAGACCACAACTGGAAACAGTTGTACAAACAGATTGCATTCA! 1 I 1 I GCTTGTATTGGATACTCGATGGCCATC ACCGCTCTTATCTGTTTCATCCTCAACCGTATTCCA 1 I I I I GCAACTGCGAGCTTCAGAAGAGGCTGAGGAGAA AGGTATGGATGAGGATCAGATTGGAGAGTTCGCTTATGACTACGTGGAAGTACGTCGTGATTTTTTGGCTTG GGGATCAGGCCCAAACAATGGCTTCAAGGAGCCGGAAGTTCTGGATCAGGTAGTTCCGGTTAATGATTTCAG CAGTGACCAGAATGTGACTAATGAGACCAACGAATCTGAGAAGCAGTAGAGTAAATATAGAGATGATATTTA GTGTATTCTAATGCTTATGTAATGTATTAAGCAAAAAGTTGTGTTTATGAGTTAGCATTTGTCTTAGCAAACAT AAAATTATGTCGACATTTGCAACCCGCATGTCTAGTG 1 1 1 1 1 AGATCGATCTTCGATGTGTAGAATAATGCCTC CACGTGATGCCCCGCGATTTTGTTGGGTCTCAATGCCTCCAACATAAACCCATCACGTATAAAAAGCCC 1 C 1 1 A ACCCTCCCCCCTGTTTCGTTTGCTTCATCACTTAACCTGAACTATCAAAP25 25 TTTTCAGCAGATCTAGGGAGGGCATCATTGAGGTTTCCACAAAAGGAAGAAACATGGATCCAGAGACATCAA CAGAGAGGGAAGCGGGTAGTGAAGCCGAAGCCACAACACAGCCCGATTTGGAAGGGAGATCACAATCAAG GTGAGTCCAGCCA 1 1 1 1 1 1 1 C 1 1 1 1 1 1 1 1 i 1 1 1 1 1 1 A 1 1 CAGG 1 AGGCCCACC 1 AAC 1 A 1 1 1 1 1 AACTGGGATCCA GGATCTCGCTGGGTGAAAGCCAACCATC I 1 1 1 G I 1 I CGGGGAACCG 1 GC 1 CGCCCCG 1 AA 1 G I 1 AA I 1 1 1 1 1 1 CTCCCGCGCAGCTTTAATCTTTCGGCAGAGAAGGCGTTTTCATCGTAACGTGGGAACAGAATAATCAGTTCATGSequence SEQDescription iDNO. Sequence TGCTATACAGGCACATGGCAGCAGTCACTATTTTGC’nTTTAACCTTAAAGTCGTTCATCAATCATTAACTGACC AATCAGATTTTTTGCATTTGCCACTTATCTAAAAATACTTTTGTATCTCGCAGATACGTTCAGTGGTTTCCAGGA CAACACCCAAAAAAAGGTATCAATGCCACTAGGCAGTCGGTTTTAI!!! 1 GGTCGCCCACGCAAAGAAGCACC CACCTCTTTTAGGTTTTAAGTTGTGGTAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCAGTGAC CGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCACCAGACAAATCAATCGA TCCATAGTTACCCATTCCAGCLl!! 1 CGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACGTTGCATGA AAAGTCCAGATTGGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAAGATACCGCGAAAAAGGTTTGT TTATAGCTTTTCGCCTGGTGCCGTACGGTATAAATACATTCTTCTCCCCCCCCTGCTTCTC H i l l CTTTTGTTAC TTACATTTTACCGTTCCGTCACTCGCTTCACTCAACAACAAAAP26 26 TTTTTGTAGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACAC CGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAGTCCCGGCCGTCGCTGGCAATAACTGCGGGCGGACGCATGTCTTGAGATTATTGGAAAC CACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAA TTTATTTGTCCCTATTTCAATCAATTGAACAACTATTrTAAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCA GGAAAAACCAGTACCTGTGACCGCAATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGAT TTCAACAGACAAATCAATCGATCCATAGTTACCCATTCCAGCCTTTTCGTCGTCGAGCCTGCTTCATTCCTGCCT CAGGTGCATAACTTTGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAAT ATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCC CTGGTrCTCTTTTrcTTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAAAAP27 27 1 i I 1 1 G i’AGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACAC CGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAGTCCCGGCCGTCGCTGGCATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTC GCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTCI I I 1 i CI I I I GTTACTTACATTTTA CCGTTCCATCACTCGCTTCACTCAACAACAAAAP28 28 1 I I 1 1 G I AGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACAC CGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGCATGAAAAGTCCAGAT TAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTC GCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTCI 1 I 1 I CTTTTGTTACTTACATTTTA CCGTTCCATCACTCGCTTCACTCAACAACAAAAP29 29 I I I 1 1 G FAGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACAC CGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAGTCCCGGCCGTCGCTGGCAATAACTGCGGGCGGACGCATGTCTTGAGATTATTGGAAAC CACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAA TTTATTTGTCCCTATTTCAATCAATTGAACAACTATGCATGAAAAGTCCAGATTAGGGCAGATTTTGAGTTTAA AATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTTTCGCCTGGTGCCGTACGGTATAA ATACATACTCTCCTCCCCCCCCTGGTTCTCTTTTTCTTTTGTTACTTACATTTTACCGTTCCATCACTCGCTTCACT CAACAACAAAAP30 30 TTTTTGTAGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACACCGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTCSequence SEQDescription iDNO. Sequence TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAGTCCCGGCCGTCGCTGGCAATAACTGCGGGCGGACGCATGTCTTGAGATTATTGGAAAC CACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAA TTTATTTGTCCCTATTTCAATCAATTGAACAACTATCGGTTTTA 1 1 1 1 I GGTCACCCACGCAAAGAAGCACCCAC CTCTTTTAGGTTTTAAGTTGTGGGAACAGTAACACCGCCTAGAGCTTCAGGAAAAACCAGTACCTGTGACCGC AATTCACCATGATGCAGAATGTTAATTTAAACGAGTGCCAAATCAAGATTTCAACAGACAAATCAATCGATCC ATAGTTACCCATTCCAGCC 1 1 1 1 CGTCGTCGAGCCTGCTTCATTCCTGCCTCAGGTGCATAACTTTGCATGAAAA GTCCAGATTAGGGCAGATTTTGAGTTTAAAATAGGAAATATAAACAAATATACCGCGAAAAAGGTTTGTTTAT AGCTTTTCGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTCI I 1 I 1 CTTTTGTTACTT ACATTTTACCGTTCCATCACTCGCTTCACTCAACAACAAAAP31 31 I 1! 1 1 G I'AGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACAC CGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAGTCCCGGCCGTCGCTGGCAATAACTGCGGGCGGACGCATGTCTTGAGATTATTGGAAAC CACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGG 1 I 1 C 1 CCTGACCCAAAGACTTTAAATTTAA TTTATTTGTCCCTATTTCAATCAATTGAACAACTATTAAACAAATATACCGCGAAAAAGGTTTGTTTATAGCTTT TCGCCTGGTGCCGTACGGTATAAATACATACTCTCCTCCCCCCCCTGGTTCTC 1 1 1 1 1 CTTTTGTTACTTACATTT TACCGTTCCATCACTCGCTTCACTCAACAACAAAAP32 32 I 1 I 1 1 G I'AGAAATGTCTTGGTGTCCTCGACCAATCAGGTAGCCATCCCTGAAATACCTGGCTCCGTGGCAACAC CGAACGACCTGCTGGCAACGTTAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATAGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAGTCCCGGCCGTCGCTGGCAATAACTGCGGGCGGACGCATGTCTTGAGATTATTGGAAAC CACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGG 1 i 1 C 1 CCTGACCCAAAGACTTTAAATTTAA TTTATTTGTCCCTATTTCAATCAATTGAACAACTATP33 33 1! I I 1 G I AGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTC CGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACG GCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAA ACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTT AATTTATTTGTCCCTATTTCAATCAATTGAACAACTATCAAAACACAP34 34 I 1 I 1 1 G rAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTC CGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTTAATGTGGAGTAATGGAACCAGAAACGTC TCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCGGGAGAGCI 1 Cl CCTACG GCCCCCTTGCAGCAATGCTCTCCCCAGAATTACGTCGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGC CCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGGCGCATGTCATGAGACTATTGGAA ACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCTATTTTGG 1 M C I CCTGACCCAAAGACTTTAAAATT AATTTATTTGTCCCTATTTCAATCAATTGAACAACTATP35 35 CAGGTAGAAAATTCACCACTGTCGGAAAGTTGTCTACTTCCGTCGGTTGGAAATACGAGTCTGTTGTTGAGAA GTTGGAGGAGAAGAGAAAGGCTGAGGAAGCTGAGTACCAGGAGAAGAAGAGAGCTTACACCCAGAGATTA GACGCAGCTAGTGCCGAGTTTGCCCAAACCGAGGAGGGAAAGCAGTTGGCTGCCTTTGGTTACTAAATAGTA AAGTAGGGTATCTTCAAGTAATAGTATACTAACCATCTGAAATAACCACCGTCCTGTAGTTTTTTTTCGATATC GAAGAGCCTATGCTAGTACTGTGGATTTGCGCTCCATCCAACATCTGTGCGCAAACTAAAACTTCCGAGACTGACATCTACCATCGCTAGACCCTAAGTAAAACCAATCTCGCGTCCGAACTTTTAAATTTCAGTCCTTAAAACTTCASequence SEQDescription IDNO. Sequence GAGCATTGGTTGTAGTTTCCGGATCTGAGGGGTCGTATTGGAGTCAAGAGACGGAGCTGCCTCCACAGCGCG AAACGTCAACCCCAACACCAACCTGAATTTGCAATCACCATGGGGACAAGTTTCAGCAGTCAATGGGCAATTC AGACGTTGATACGGTACCCATTTGCTAAGCTCAATGACGATCCATCCAACTTCAGAGAAAGGCCTTTCTCTGG TATGCTCTGGTATTCATTCGTCTTTTATCACTCTCGTTGCACAATGCCCGGGTACTCCCGGAACAAGGGAGTCT TCCAGCCAAGCTGTACAGAGTGAAAAATAGAAATACACCTTTGCAATCAAGACGCGCGTTGGCCAATCACAA GACTTAATCGGTGCAAAGAAGGATTACCAAATTTTTTTTTCCCAAAATCGCTATATAGAAATAATGGAGGAAA AAGGGTTAATATAAAGGAGAATTCCCCCGTTTTTCTCCCCTTGTCTTTTCTTCTTCAGGCTTTCTTACAAATCTAT AATATTCCAAAATGGCTGACAACAACAAGTCTAACTTCTTCGTCGACTTCP36 36 GACTGATGAGTCAAGAGCAGAACTTGCTGCTGAAGGAGTTGC 1! 1 G I AGTGTTCGATCTTTCGAGTTATATTA TTATACTAGTGGAAATATAATTATTTTGTGATGGATTGTGTAAGAACAATAAGTTTTCACATGGAAACGCAATA TTAAGGTTTACCCATTGAATTAGATCAGACTGTGAAACAAATCAGCCTTTTTAAACAGGAAATGTAACAATCTA TCAATTGGAAGTTTCAATGGTGG I 1! 1! GGGCGCCTGAGAATAAGCTCTTACTATACCACCACTCTATCATTTA CCAAGACTGATTTGCCTCAGGGCGTGTGGTCTAGCGGTAGGATTCTCGCTTCGGGTGTGAGAGGTCCTGGGT TCGATTCCCAGCTCGCCCCGAGTTCAA 1 I 1 I 1 1 C I AAGAAA 1 GAGA I I CGAGCA I CGAACG 1 1 I 1 I 1 CACAAGTT 1 I I I 1 CTTGATCTCAAAAAAAATTTCAAATCATCGTTTTCAGCACCTACACAGATTACAAACCTAATCTCATCAG TCTCCCGTCCAATAGAAGTTGCCCCATTAAAGCCGACCTCATCCCTCATACGAGGGCAATTGTCGTACATGATG TCTCCTTGTTCCTCCTCCTGCATGTAATTAAAGGTCGTTTCTTTTTTCTTTAACGGACTGATCCCCCTGAACCAAG TGCTTTCCGCTAATGCCCTCCTAGTGTTCACTGCTCAAATCTCATCTTTGTTTCGCGTCAGCCTTCATCCCTACTT TTGTCAGCGTCTACATTTGAAAAAAGGTAAACAAAATCGCGACGAGATTGTTTCAAATGCAGCCTCACAGGAG GAGATCGAGTCTCGATGCTACCTTGGCACTAGGTATCGGCCTTTTAGTGGGCCAAAATTCTTCCCTGTTTTCTC TTCTTCCTTTTCTTCACATTTTGACGATTTTCATACAAAAGGTCACTATATCCTTCACTCACTCTATTTTTTAAGTGH i l l CCCACACTAGTCATTCCTTTTATCAACCP37 37 AACACGAACACTGTAAATAGAATAAAAGAAAACTTGGATAGTAGAACTTCAATGTAGTG 1 I I L 1 A I I GTCTTA CGCGGCTCTTTAGATTGCAATCCCCAGAATGGAATCGTCCATC 1 1 1 C I CAACCCACTCAAAGATAATCTACCAG ACATACCTACGCCCTCCATCCCAGCACCACGTCGCGATCACCCCTAAAACTTCAATAATTGAACACGTACTGAT TTCCAAACCTTCTTCTTCTTCCTATCTATAAGAP38 38 GCCATTATCTGCTGATGGATTATGACTTGCAGTGATCATAATTCCACCAAAAGATTTATAGTAGTCGACAGCAA ACGGAACAAATGGAGTTGGGATATCACCACCTTCTCTATTACTTACATAATACACGCTAAATCCI 1 1 I AGAATG AATGCTGTGGCTGTGTATTCGGCAAACTGTTTAGAGTTGTGACGATGATCATGTCCAATAACTATTGATTTACC GCCAGCTTTTGACATATAGTTGGCCAGTCCCTGAGATGCTTGTATTATCGTGAGATCATTCATTAGACTAAAAC CAGCACCCATTCTACCTCGAAGTCCCGCTGTTCCAAAAGTAATTCTTCTTCGTAAGCGTTCTTCAAGAACTTTAA ACTTTTCATTCTCAAGAAGTTTGAGAATCTCAGATCTAGTGGATGGATTACGATCAATGCTAAGCCATTGATGC GCTAACGCCTTAATTGATGGGGACATGATGTCAGATGTAATACCTTGTTTACCGAAATTTAGCTATCTCACGTA GCCTAGAATGGCCGCTGCTAATCGGCCTATATCTAAAACGGCATATCGAGGATCGATGAAGTTTACACGTGAC GCATTCAGTGTAAGTAAATTACATGCAGTGCCGGCTACTTGACTAAAGTAAGAAAAAACTCAGAAATCAAAG AAATAACAGAAGCTGTACGACACAACGAAATAAATACAAACTTTCTTAGGATGCAATTTAACAAGAATCGTCT TATTGTCTCTCGGAAACATTCAAGAATTTCAGCCCATTATCCACATTCAGTCATGGCGCATTATTTAGATTGTGT TGTTTGATAAAAAAAGAATACAGCAAGTAACAACGAGTAGTCAGTTACTGAGATATTTTTTAGTACCGTCGAT CCGGTTACGGGGAATTTAACTGCAATATATCAAACGTTAAACTGGATCGAGTTCAAAAAAACAAGCTCTAACT CAGTCAAGCTTTATGTATAGTTTCCTAATCAGGTAA 1 I! 1 ITP39 39 CCCATACTAAATGTGTATGATCATCGTCGCACGTTTGCTTTGCCATGCAGGAAGACATAGTTACTAATAATTGG CCCACCTACCATGTCAAACTGCTAGTGGAAACAGATGCACAGAAGAATAGTTGCAATTGAAAAATGTTTGAAT TGTCATGACAACTAATAACAACTAGCGAACTACAACTAGGCCAAGAAAACTTCCTTGCTTACTCTCGTCATATT TCACTCTAATACGGCATCCGTACTACGTTGAAAGAACACTTGCAAACCCGAAACAGGTGCAGTCTAAAAAAAG GCCACTCACTCCTCTTCAACTGGGACAAGTGAGGACAAATCTAGCAAGATGGCTTGAAAACTTGAAGTCGGA AAACCTGTCAAGCCTAAATCTATTAATAACTACTCCTAC! 1 I 1 I CGGCACAAAGC 1 GGGCA 1 1 1 I UI CCTCTTACTATCATTTACCTCCTGATAAGATCGTAGCACAATTTCAACGCAGATTGCTGTCGTCTTTGAACTCTCAGTCCCTCSequence SEQDescription IDNO. Sequence TTGGCCTACAAAGGTTAAGTGAGATAGCCAATTTCTAGACCACCTGAAATTCCCATGTGACCTGGCATGTGAT TATGCACGTAATTACTGTGTAGACCTAAAAATATCTCATCTGACAAAACGGCGTCATAACCTAAAATACCTCAG ATTTATGCAACCACACATTGATTTAATTACCAGAGAGATCGCTAACGTTCTGAGAACTATTTTGTGTATACTTC CTGCATCATCTGCCAATTGTTGGATCTCCCTAAACACCCACGTTGTCACGACAACTTATTAATGGGAACTAATA ACAACAGATTATGCTACACGGGTTGCCCGTTCTTTGCATATACCGCCATGAAACTTTCATACGACGTGCCTGGC TGTAGCGTGTGGCATCAATCAGCTTTTTTTTTAATTGAAGCAGGAGCGGATCATCTTATCGTGTAACCTTTTCC AGACTTCTCTACTGCTTCCTTAGCTTTATTGCACAACGGGGP40 40 CATATCTCAAGGCATCAAAGGTTCTGGATCTCTCGTTGATACATTCCGCGATGGGTTGCTCTATTCAAAGGAAC TTGCTGACCTGAAAAGGTTTGAATATGCTGAGGATCTGGAGTTTTATGATGCTGAGGAAGAAGAACAGAACT TTAAGCAAGGGATCACTGGCAACGGAAAAACTAGCAAACACCAGAGAGTCTTATCCCAAGATATCTCCAATA ATTCCGTGAAAATGACCCCAGTAAATGGTATCAACAGTGATTCGTATGTATCGGTGAATACATAGTACCCTCCT ATTTAGGCAAATGTCATGCTTCAAATATAATACCCACGTTTGTTATTGTTACCCCTATCCTTTTGTCTAGAACAC GTTTGTCGCACACCAACTATGCCTAGAACCCTGTAGATCACTCTCATTGGCTCGAACGATGGGGATACAGAAT GAAACCTGGTGGAAAGTTGTTTACATTTGGAATTGAGTTAGCCCCTGTGATTATGACAATCTGCATGCTGATG CATTTGATGCATGTGCATTCTAATCTGAGAATTCCACTCAGTAGATCGCAACAAAGAAGACAAAAAAGTAGGA CAATTTCTCTGGTAAAGCGAGTTGAATGATCAAAGTTAAGATAAGCTAGCCTATTTACACCTGTTGTTTCTCTG TTTGAAAAGGCC I i I 1 I 1 G I GA 1 GA 1 1 1 1 I i 1 i GACTCATCACGTTATTCCAAATTTCACTCGAAACAATAGGTC GTGCAGATGCATTTTCATACACTTAAAATCAGTCTTCTTGTCTAATATTAAGAAACGCGTTATTTACGTCTCCCC CTTTTTGGAACCCTGGCACTCAGGGTTTGTTAGAGAAGTTATAAGACTCCTGTGGTTCCCTGAAATCGTGTTGT TTGTGA 1 1 C 1 1 1 1 1 GA 1 ACA 1 CAAG i Cl 1 1 1 i CAAAAAACAAAAGAAGATACTCTTGAGCCGTTTTCTTCAAATC CCAACTATCTGTCACTCCTTTAAAACACGTTCACTATTCCAP41 41 GAGGCCAGCGCTGCGGCCGTCTGCGGGCAGAAGGGCCAGAAACGGCAGCCAGTGGGAGTGAGACGAGCGC CGCCCGCCTGATTGTGATGATCTTCCCCCCCGCTGGGCCCACAGATGGCGCCAGGATCACCACCGGCGTGCGT CGTGCTACATAGACGGAAGGCGAGGGGAGGGCGAGCAAGAAAATTTTGTCGCTTTGCCGTGCGCTATTGCG GTGTGCTTACCAAGTGAGGGGAGGGAATGAGCACGCTCGAGGAGCTAAAAAAAAACTACAATAACAAAGAC AATAAGATGACGGTGACAACTGCAATGACAACAACGACGATGACAACCGGCGTGGTATGTAGCATTCGTAGG GCATACGTACGTTCATCACGCCTTGGCCTTGGCCTTGCCCACGCGCATTGGTAAAAATAGCACGCCCACGCCC CCGGCAATAACTACAACAACAACGACAACAACGACAACAACAACACCCGAGCCGTAGAAAAGCGTGGAAAA ACAGCAGGCAGTAGTCGCCGCCGTCGCTGGCGGCCCCCCCAACACACGCACGCACACACAGCATGCTGCCGG GCCATGCTCGGGACGCAGCTGCCATTGCCACGGGCTGCTAAAAATAGCAGCACTCCCGGCTGCCGGCTGCCT GGTTTCCCAAGCAAGCCGGCGCGCCTATGCCTCGCGCCGTATTTGTG 1 1 1 i 1 CCCGATGCGGTACCGGTGACC TCGATAGGGTACCGCGTTGGGTGGAAAAAGAAACAAATCGCACGGAAAATACTTCGGAAGAGGTGGTGGGT AGCACGCTGGAGCAGGCTCGAGAAGGCTGGATGGAACTGGTCACAGAGGGTCACCTTTTTTCTATAAAAGCA TGTGGTTCCCACCC I I i 1 i 1 C 1 i C 1 C 1 CA 1 L 1 GGA 1 1 CCA I H C l I CI I I I C i C M i GCCTTAATCTATTTTCTTCTC TTCTTTCTACAACAAACATCTAACTGTTCTAGTCTTTCGCTTATCAATCAATCCATTAACAP42 42 GATGAGTCCTGAGCTAGTTTCACCGTTCCGGTGGGATCCACTGGAGCAGACAAGCTCACACTTGCGGTTGTCT CGTATTAAGCAATCCGACAAGGCGATATCGA 1 1 1 1 I AGGC 1 CAGAGCGGAG 1 ACA 1 CAC I 1 CA i CA I G I 1 1 I 1 G TTATTAAACTTTCATATTAAATAGCCCTCAGTGCCTTAAACCCCACCCAGTAGTGCACCGGCTAGTGGGTATGC AGGAGGGTCCTGCAATCGACATCTTGATCTGTTCTTGCCTTGTTCCGACGGCATGATAAGACTGGCATTAAGC CAGAAATTACCCTTAATGGTTTTCTCACTAGCCATCTGAATCTCAACTTCCAATGTTTTAGGTGAGAAATGCAC CAAAAACCACCATATTCTCCCGAAAAGGAAAAAATCGCAACTTGAGGTCAAAAGCAACGATCACCAGTCTATT GTGGACCCCTTGAAAGAAAGCGGCATTGGGTAAATAGCCCTTTGTGATGGGCTGCTTCCCAAAGTGTTTCATC AACGGACCCATCTGAGTCTCCGGTACCCGTCATGTAGCAAATAACTGAAACTGGAAATCTATACACACTCTTTT CAAGCCGCAGCGTAGGTCTAGCCCCAAGTGGAGGTGGTGTGGTTAGCGCTTGCCAGCCTTACCAGTCAGCTT TTTCACTGCACCAAGTGCTCTAGCAGTGTGTTTTCACCTCTTCCCAATTCGGCTAAATTTAGTTATGCTCCACTT GCGGTTTCAAGCACTCCATGCGCTATCACCCCCAGGACCCGCCTTTAGGGGCTATTAATAGGAAAATTTCCGGGGCGCCAGTGTTTTGTGTGAACTTTGCAGGTTTCCAAATAAAATTCTTCCTGCCGGTGGAAGTCTGCCCTGTGTSequence SEQDescription IDNO. Sequence GGTATATAAAGAAGTGTAGCCCACCTTTGGAACCTTTTCTGTCAGAATCCTCITTCTCCATATTCAACCTCGAAT TAATCCTTCCCAAAAAGTCTCTCGCTTCATACCAATCACTACAP43 43 ATAAATAATTATTTGTTCATTTAAGTTGTATCTTTGATATTTCCTTTATGGAAGGGACCGCAAAAAGACTTTGAT TATTATTCCTCACCATTGCTA 1 1 1 1 i 1 i 1 i 1 G i 1 AAGA i A 1 1 AC i 1 1 AAA 1 AGC I l l i GTAAATTAATCAGATTAA AATTGCTGGTTAATACAACTTTATTGACATTTTAATGAGTCAGTGAGTCAGTTATTCTTACAGTATTGCTAAAC GTTATAAGGTATAATCAATAGTATCTCGCTTTCTGACTACCTCACTCATTACTAATAATCCAGTAAGTAAGATGT ACTTTTCAAACAAAAAATTGTTTGTTTTCATTTCATGTCCAAGCTGCATTAATTGTTAGCGCCATGCATAATGTT ACATTAAACAATTGTTTGATCATACTGATTTTCTTGATTATTTGAATAAATTCTTTGGTTTGCTTGTTTACCTTTT AATCTTTCATTGTTTTCAACATTGTTGTTTGCTCACACAAAACCAAATACAAACAAGTAAATAAGGAAACACTG ATAATAAATACACTAAAAATAAATAGCAAAAAAAAGGAGACAAATTCAAAAGAAAAAATACAGACAAAAATA ATTTAAAAAAACAAATAAAAAAAAAAAATAAATAAACAAATAAACAAATACTAAAAAAAGAAAACAAACAGG CCTACAAAAACTTTGTTTTGTTTGTTTTCTTGCTTCTCTGCGGTGCTGCCTATTAATGTCCAATTTCTTTTGTTTTC CTTCTTTCTGTCTTCCTTCCAATTCTTATGTTTTCTTGTTTTCTAACACAGATTACATCCATCACCCTACATCCACT TTCTGTTTCCTACACTCACTTCCTCTAC 1 1 1 I I I 1 1 1 ACTATATAAGGTCGGCAATCCCTCCATTATATCTTAATAA TTTTCCATACTCTTATAAATCTTCTTCTTCCTTTTCTTTCTCTAATTTAGATTCATCCAAATTCTAGTCTCTCGCTTT AGATACCAATAAATCAACAAAAP44 44 CATCCAATCTTTTCGTAGCGATCTCGTTCTCAACCGTGGTCCGACTTCCATGCCCTGAGAGGTAATAATACCAC GCCGTGTGGTGGCAAATTTCCACCTCAAGCTGGATACGAAGCTCATACGGAGACCGTACAAAAGAAGCGCAA TGGGAAAATCGACGTGGGCTGTCCCTC 1 I I l l i 1 I GCGCCC I I CCCC I I I 1 C 1 C 1 CC 1 1 1 I I I 1 I CTTGTATCCAC TCCGCCCACCCCCCCTGCTCTCTGCCCACACCATACGAGGTGCCCTCCTAATGACTGACCACCAAAAAAAAAG GGAGCAAAAAGGAGATTATGGATCACCCATTGCACGGATCTGGGTTAGTTAGCGCTGGTCAATCTCCTTTTTT TTCTATTTTTTCCTATTTTTCTTTTCTTTTTTCTTTCTTTTCCTGTCTGCACGCCCATCCGTCCCATCTTGCATCCGC TGGGGCCCCTG 1 1 1 I I 1 I 1 I LAG 1 L 1 1 G 1 L I 1 1 G 1 1 G 1 1 1 1 1 1 1 AA 1 LCA 1 1 1 C 1 1 1 A I I 1 G 1 C 1 1 1 I 1 I AGCATGG 1 I 1 1 1 1 1 1 1 1 CGGGGCCGTTCTTTCGGTTGCTTAGCTTCGTTGCTGAGCTGCTCGTTCTCCCCTTTCCGTTCCCAA TCTTTTCCCCTTCCCCTGCTACCTAATCCCAGCCCACACGCACACGAGCACACACACACCCCCCCTTCTTTTCTTC CTCTCCCCCCCAAACCAAGAGGAATGTGGGCGAAGGGGAAAACACAAGGCCAATGCGGTCTCCCATTCCTGT ATATAAGACCTCCCTTTCTTACCCTTCCAGCCCATTCCCCTTTCTCCTTCTTTTCTTCCTTCTTTCTTTTTTTTCTCTC TTTTCTCTTCACCCACACTCACTCTTACAATTAATCACCCTGACTTTTACAGTCAATTTCACTCGTTAAACTCACC TCAACGCCAAAAACCAAAGCTTTTGCTCTACTCCAATCTCCCAATTTACAACCCAGTCAACTCGACCAGGAAAA CCCAACCAGTCCCCAACCGTCAAAP45 45 ACAACAATACTGCTTTTGACTCCAAATAGTACTGTAGCATGTAGTGATATTGTTTATTATATCTTCTACTAATAT ATTAATTATGTCTTCTGCTAATATATTAATTATGTCATTACACCTAAAACATTTTAAGAATTAATCCCGTTGAGA AATAAACAAAAAGTATCCCTCAATACTACTCAATTAGGTAAATTAGCACCCGTTGCAGCCTTACCATTGCCATG GCCAGATGCACGATAATGTATATAGAAGGACAAACTTGCATTAGTACCACCAAGGCAATAAGAACACCAGCC ACTGTAGACGATCTACACTTACAAGGGGCAATTGCTAATAAGTAATGGCAAATAAGCAATAAGCGGCGAACA ACGAGATACTATTCATGGAGAACCTTCGGCAATACACGAGAGATTATAGCAATACACGAGAAACCATAAGAT ACTAAGATAAATCATGGCAAATCATATTAACTATTGACCTCTGACAAACAGTTATGGCCCTTTAAAGAAGGTA AAACGTGGGAAGCCTTGGGACAGGAAAAAAAAAAAAAAACCTTCTCTCTCAATGAGCCAACTTTTCATTACAT CATCATCATCCACGATTTAATTGGACAATAGGAAAATGCAAAACAAATAAAGCTGAGTAAAGAGCGGCAAAA ATATGCAAAAGAGACAAAGATTTGCCAAAGAGGCAAAGATCTGCAGAAATGGGAAAAAAAACTTCATAAATT GCAAAACGCGCTTCTA 1 1 1 1 I AGTACATTCGACAGCGGCCGTGCTGTTTATCTTTTGCCGCTTACGGAAGGCGC GCGCCGCCGGTGGCTGTTTTCTGGTAAAGTGACTCTTCCACGGGGGGGAAGCTATAAAAAGCGTGAAATCCC TCCCACATTTTCTAATCCCAGTGGTAACCCCAACTTCTTTTCTATATTTTnTTATTTTTTTTCTTTCTCACTTATCA AL M M ATCGTTCATAGTCTCTCGCTTACAAACTAACACAATAAAAP46 46 GAAAGGCCAGATGCGTGTGTGTCCACAA H I LI GTGCATAACTTCGGGCAATGGAGAGCGGACCTTTCAATCTGGCTGGTGGTGATACAACTTAAAATAGAATTTTCAGGAACGAAATGAGGATTACGACTAACTGATATGGCGGTGTCGGGGTGATGTTTCTGCTGGATGATGCTGCCAATAACCCATTACTTCAGGTCACAGGTGCGGGAGGCCTSequence SEQDescription iDNO. Sequence GGTACTATGTTTTTCACATGGCGGAATTTCTAGTAATTACAGTGCGTACGTACATACGATTGCTTCGTATTGCG AATTCCGCACATTGTCCATAACTGGTGTTAAAAGATTGCTTGCTCATCGGCCCTGTAACTACTATGCCCAAATT GGTGCCCGGATCGTCGTCGCATTTGCCTGTCAAGCACACCACTGGCAGAACCCGAGGCGTGTTGACCAATCAT GGTCCCTCGATGGTGCCTGATTGGGGAAACTCGTATGTTCTCCCTGAAACATAGACCTCGATTGTGTCAATTCT GGAATACGTGGGGGGATATTCCCCAAAATATCTAAAATATGTCTAAAATAAGTAATTACTCGAGCAAAACTGC CCAATGCGGTAATTGGATAAGTGGGAGAATTTTGCGACTAAGCATTCTTGTCGCCCAAAATAGCATCAAAACA TCGATGTCGACCCGGTCCTGACTCTTTGAATCGTAAAAAAATTTCTTCAAACTACTAATTATAGCAGATTGTCG TGCAGAACCCAGCAATCTGCCCTGAGAGTGCGTCTCGAACAATAGTGCGTTGTTTCTTTTCAGCCCACCAATA ATACTCCAACAATACCAAATCTCAGTTCATATCAACTGCTCCGATTATTTGTTTACCTTTCGAGGCACAGGTAAA TTCTTGC H I CCAGAAAGGGACTGAAGCCCAATGTTTTGGGAGGTATAAAACGGGAGCATCCGCTAGGTAA ATTCTCAACTAACTTTGTTAATTCAATCGTCTTTCGCTTAATTAAAAP47 47 TTCATCAGTTGCCCATTCACTCTTATCTGGCTTCAAGGCTTTACTGTTTCCCCTTTAGCTATCCTGATGCCCTACC AAATCGATTGTTGCCCTTACACCAATGTACGATCACAACAATTCCCATGTTGTTAGTCCCTACCATTTGCGAAG CTCGGAGGCCTAGGTCGTTGAACGTCACACATTACAGCTGCTCCGAAATTCGCATAAGGAACACTCGCACCAA TCACAAGGTGATGGAAATTGCTTACGGAGTAGAAACTCCCAATCCCGAAGGTAAATACTTTTCTAGTGCACCC CGACATTCGACTCAAAAGGCTTAAACTAAACTCCTAAAATGTCCGTGGTTGACCAATAGAAAGTATCACTCAG CTCCCTGATTGTTCATAGCCTAACTGTTTCTGAATCTCTCCAAGTTTATTGCTGTCGGGTGAGCCTATGATTATC CCCTTTCACAATAGGCTCATTGTGTCTTAGGAAGTACCTGCCCACTTCCCCCTGATAAACTTTCCACCATCCCCG GTCATTCGCTCATGACCTTCTTATTACCATGCCAAAACATCCCATAATGAAAGGGTATCGGCAACATGGGAGC TAAATTTCAGACCCTCGAGATGGAGTCGGTAATCGTTCGAGAATCACATGGCCACCCCACATTTCAATTGTAG ATCAGACTGTCAATCTTGACATAACCGTCGATAAATGACTTAGATTTCCTTCAGATACTCAGATTATCAGTATCT GGACTCTCCTGACC H i 1 i L 1 A 1 i 1 GL 1 LLAAAG 1 C 1 1 LGAA 1 L 1 1 1 CL 1 A 1 L i 1 G 1 i GGAGTCTGACAAACTACT TTAACGTTATTGGCTGAATTCCACCCTCGGATCCAACTTCTCCTTTTCGCTACCGAGCCAAGGCAAATGTCTGA TGCAGCTAGTTnTACTGGCATACCAGGAGATCGCATTCGGGACATATATTATGGAAGTTCCCTTCTnTTTTTC CTTC 1 1 C 1 i 1 1 1 LLC 1 1 1 1 GTTCCCGTTATACAGTP48 48 TATTTTGTCGAATGTTGCTGCTTAGTAAATTAGAGATACCGATTTGCGCGAAAATTTGACACTAGATAAAGGG TGCTGCGAACGAAACACACTCGCACATATCTTACGTAATACATTTTTTGTAAGTTCCACAAAGTCTCTATGTAG TTCCCGACGGACCTTCTTGATACAGGCTGCGACCCGCCTACCGGTTATCAGCTCATCGGGTCAGCCCATCTCTA CGCGCTTGTCCCGTACACCTAAAGTTTCAGTTCCACATAGACCGGAGTCCGCTGAAATCTTATTGCAAGAAAA GCGGGCAAGCTTTTCGCTGTTTTCTGCAAGTTAAAATTTGCCAGAAAGTAATTTATTAGAAGCAGCGAAAAAA AGAGGGGCACTCAGGCATCTCCGAGATACCGACAGAGGGCGGAGACAACTGCTGCCAAGACTTCTGAGCAA TTGTATGGTAGCACTTACTACTATAGTAGCTAAATAAGATATGGAACATGCATTAAGTATCGCCATCCACCCCC CGGACTCCACATTACGCTTTACTCCACAAAATCGGCACAGGGGAACGGGGGGCGGGTAGATGAAGTACGCCT GAAACAATTATCCTAATCCCCTCCTACATTAAAAAAACCCCTTGGATCTTTGTCACCCCCCTCGCCCTAAGCTTT GGGGTTCTAGTGATGCGTAGGGGAAAAAAACCCTTAGGGCCCCAAAGGGACTAAGGGATCACTAATAGAAC AATTTCACACCGGAGCCCGCGATAGAAATAAATAAGGTGACCGGAGGGACGGTAACAACAGTCCGAATGTT GTGCCAGAAAATGGCTTTTACCTGGGGTTCTGTCCCGCATTGTCATCCCCAAATATGACACCGAGAATGACTT CTCGGAGCAGAATACTTATCCCCATCTTGTATATAAATAGAGAATAATTTTCCTCCAGTTTACTCAATCATTCAC CAGACAAGCAGTACAACAAGAGCTTCTTTCTTACTACACACCTTCTTAATTATAAACTAAACTP49 49 CCATAAATATGGGTCGTGCACTACACACCCCCTTGGACCGCCCCCCAGGGTTTCTAACCGCTCGAAATTGTGA ACTGTGGTTGGCCAATCCAAGCGCGCCCTCGACACTCCTCAACGCTGTTTTTTATATTAACTAACCCCTTCCTTT CCTTTTCTCTCCTACACCCAGTGTTAAATCTTTATCTTTAATAATATTCAAAGATGCTTTCAGCACGCCCCGTCAT TAGAAGAGCTGCTCGTTCTGCTGTCGCCATCAATGTCGCCCGCTCTGTTCCTCGCGTGGTATGTATTTCCTGAT GATTTGAATCAC 1 1 1 1 1 GA 1 1 1 GGC 1 G 1 GAAL 1 GGL 1 LGCLLA 1 GAAAAG 1 i 1 G 1 CTTTACTCAACGTTGAAGC ATTATTGATGATTATTTTGGATTGTAGAGGATCAATTCCATAGAATGCTTTAAATATTTCACACATTAATGTGTT TTCGAACAGGACAATCGCTTCAAATGGTGTGGTGAAATCTATCAAAAGTGTACTTTTTCTTTCCATTGACAACTACTAACATATCATTAGGTCCGCCCATTAG H I L I AGAGGTTACGCTGCCGCCAAGGCTGCTCCAACCGAAGTTTSequence SEQDescription iDNO. Sequence CTTCCATTTTGGAAGAGAAGATCAGAGGTGTTTCTGCCGAGGCTGACTTGAACGAGACTGGTAGAGTCTTGTC TGTTGGGTAAG 1 1 1 I I U I GAAI 1 1 1 I I 1 i I GAATATGTCATGGGCTGATCTTAGGGAGGCATGATACAACAGTG CTTTACTCCACAATTGTGACGTATTGATTGATGTGTTGTCCTCAGATATCTTCTTGTCCAGATCCATTTTTTTTTC AAGTTTTCTCGACATTTTATTGGACAGTCGGAGATGCTCGACTTGACTCCCTAAGAACTACGCCCTTACAAACA ACCTTAAACTGAAGCTCAGTAAACTAACATTATAGTGATGGTATTGCCCGTGTCTTCGGATTGAACAACTGTCA GGCTGAAGAGTTGGTTGAATTCTCCTCAGGAGTCAAGGGTP50 50 GCTCCTATGTTGACGGAGAGTGTTGGGTCTAACATCATGGCACATGGTAGAGGAGGTGTTAAGACATCTAGA GAGCGTGATAGAGAGGAAGAGATCAGAGAATACGAGGAGACCAATTTCACTAGATTACCAACTTCGGTTACT GAGAAGTCAAAGAAACAAAAGAAAGATCATAGATTGAACACCTTTGCAGGAGAGGATTGGTCATTCTTTGGC AAGGACAGAGATGAAGACATGAAGAAAAGTGCGAGGAAGAATAAAAATACTGCTTCCTCCGCCTGGGAAAG AGCAAAAAGACGCAGAGGAAACTAAAGTGTGTAATCATATATATAATAAATGAGGAATAATAATTGAATAGA GATTTAACGAGTCGAAG 1 1 1 C 1 GAAATATACGCACAGTTTATATTTATGATTTTGATATCTAACTACAGTCTTCT CCATATATTTAACTATAAATAATAAAGTATATAACTCTTATGAAACTGTTTCACCACATTTTTTTCTACGTAATCG AACTCCGAATGCGGTTCTCCTGTAACCTTAATTGTAGCATAGATCACTTAAATAAACTCATGGCCTGACATCTG TACACGTTCTTATTGGTCTTTTAGCAATCTTGAAGTCTTTCTATTGTTCCGGTCGGCATTACCTAATAAATTCGA ATCGAGATTGCTAGTACCTGATATCATATGAAGTAATCATCACATGCAAGTTCCATGATACCCTCTACTAATGG AATTGAACAAAGTTTAAGCTTCTCGCACGAGACCGAATCCATACTATGCACCCCTCAAAGTTGGGATTAGTCA GGAAAGCTGAGCAATTAACTTCCCTCGATTGGCCTGGACI 1 I I CGCTTAGCCTGCCGCAATCGGTAAGTTTCAT TATCCCAGCGGGGTGATAGCCTCTGTTGCTCATCAGGCCAAAATCATATATAAGCTGTAGACCCAGCACTTCA ATTACTTGAAATTCACCATAACACTTGCTCTAGTCAAGACTTACAATTAAAP51 51 GATAAAGAACGACCGGAACCAATACGGGGTTGTGCAGGTGGGAATAAATATGTTGGTTTGGA H UH GACGT GAAGAAGGTATTCTAGTCGATGAAGTGGTTGATAAGGATATGGCGTCACTGAGTTGTTTTCTTTTCCTATGTT GCGGTGTTGGGTCAGGAGTTAATTGATTCACCTCCATAACTCTGGAATTTCTTGAATGTGGGGTTTTCAGATG GGCATCTTTCTTGACGGGGTTGTGAGTAACGGAGGAACCTGGTGTCTTGGGTGTGAACGGTGTTTGAGCCTG TACGCGGTTACTTCTGGGCGGAGTACTCGGAGTCATGAGAGCCATTGATTAGAAGGTGAATGAGGGAGTCAC CACTCTAAGCAAACAAAATGAGGTCGAAGCAAAAAATAAAGTAAAGTAGCAC 1 1 U I GGCAGGTTAGATCAAA GAGTGACGGGAGATTTGAAGATGGCTGG H i l l CCTTAGTCTTGGAAGAGGTTTGTGTGGGTATCAGCGAAT ATTCCCCGATTAGGCAAATTAGTTGCATTGAAATTAACACGACATGGTGATTTGTGGTAACAAATATCTATTG GTGGTTGGTGTGTGGGTGTAATAGTGGTCGTGTCATGATGATGGTGTTCAGGTGTTGTCATAGATCGGTCTTC AGTAAGAGAAGGAAGCTTGGTGACGATCACAGCTATGATGTAATAGAAATTGCTAAGCAATTGTGAGGTGTG ATGTATTTTGCAGAGCAATTGTGCGGTACAACGGGGTGTTATTGTCTTCACAAGGCATTTATTGCGAATTTCGT AGTTGAAAGAATATTTTAGCACAGGGTGCTTGACCCCTATTGTTGCTCGCTAAACCATGATTGCTAAATGATG ACATAGCAATCACTTTACTAAGATTGCTATAAGGACACCTTTCTTAGTATAAATGGACACTCTTTTCCCCTGCTA AACTTCTTTTA H i l l CACACTTAAACAGTTACAAAACACAAACACAACTAGAAP52 52 TTTATTTTATCAATCCCTGGATCTGCCTGTAAGTAATACTACCGCCGAAAAAAAGAAGGTTCGGACCGGGACC GGTCAATCCGACTTTACGTTTCTGTTGTGCACTGGATCTATTCTAGGCACATATCTGAATTGGAACGTGTCACG TTTCGTAGTTATGACTAAGGCGATTTATGAAGTCATCACCAGATAATCTCTTTACAAGGAGACGGACGCCCTTC TACATACACACCTTCATCCTCTTGAAGGGTAGGAAAAAAAAAAGATTGCAGCACCTGAGTTTCGCGTATGGTC TCCCACTACACTACTCGGTCAGGCTCTTAGCAGCTTAACTACGGTTGATCGGACGGGAAACGGTGCTTTCTGC TAGATATGGCCGCAACCGAAAGCTTTCTTTTACAGCTAGCTATTACCAGCTTACATGTATACACAACAATGTCC ATCATAAATCAAAAAAGAGTTTCTTTACAGAGTGCTTGTGTTTCCTTGCGGTTTTGTGTTTCTGATGATGGGTA ATGATTCTTTAAAGCTAAGATGATTACATGAAAAAGGCTCACCACTTGGAGAAGGGCACTCAGTTAGCTAACA TTCAAATACGGTTTTGGATCTCATACCATGAAAAGTAATGTATCAATAAATCAGCTTCAGGGTTCGCCCCCACT ACTGGTGATCTCATATCTTCTGACCTTCAACCTTGCAATAATTAGTTTACCCAAGTATGAAAGTTCTCTCTTTGA TAGTCTCTTTCAGTGCCTGCAGTGTATAAAATACCGTCTATCGCAATGGGTGTTCGACATTGTAACCACCGAAAGCAATATGCTACCAGTTGGTAGTACCGCTGATAGTAGCAACTGTACCAGCTAAATCATTGGATCAATCCACATSequence SEQDescription iDNO. Sequence ATTATTCTCGCTAACCACTAACAACATCCAAACATCATCTCAATTCCACCAATAAACGCGCGCTCATTGACAAC AGAAAATCAGTAATCTTCAGTAACCTCAGTAATAACTCCACCATAP53 53 TTATCCGATGCGCTTCAAAGCTGGAATTGTAAATATAGAGAAAAAGAAGGATGTTGTTTTA i 1 C 1 1 GAAAGAG TATAATTTTACTTCTAGCAACTCTCCCACTTCGCTTGACTTCATTTATTTCTTGGGCACATAGGCGTAGTAATCT AGACCAACAGATAATTTGCCGGAATGATATAGCGATTGGAAAATGAACTGAAATTTTTTGCTGTCTTTCAATTT GACGGGCAGTTCATCAGTGACCGACCATATAAATACGTTGAGAATGTTATTCTTCCTCGTAGTTGAAGTGGCT TCATAATTTCAGAACTCAATAGATAAACTAGGATGTTTTAAAGCAATTAATGCTCACAAGTAAGGAGCGACTC TCTTGCTTTTCGAATACTAAAAGTATCGTCCCAACCCAGAAAAAAAGACCTCTTAACTGCAAAATAAACTCTAT ATATTTCTTCTAAAACAGTTTCAGGTTGGATAGTATCGCATTCTCATCACTTCTAACTAGTAGGCCATGAGATA TATTAACGTTTACTTGAGTTCTAAGTTCTCCGAATTAGATGCACAGCACAAACAAGATTAGGTTTCACTTGGTA CAAAATACGAACAGAGTTTAAGGTCGTAATTTCATTTCGTTATTGATCCCCACAATCTATTCTTATCACAGTCAT CAGATAGTCGCGAAAAAGCATGCAGAAAAGGGGGTCGTCCCTATCTAAGTTGTAGCATTACAACAAATATGA CTACACTCAGTGTCGCAATCGGTATAGCCAACGCTGCAAAATGGATTCTACTGAGAATGGTATGATGATCCCA GGATCAATTTCCCAAAAATTAAAAAAAGTAAAATAAAAAGCATCAGATATTAGGGAGGTGGTAAGATTGCTC TGCAAGCGATCACGAGATTTTAGGTTTTCCTTTATGTACTATATAAAGCGCAGATTGGATGCCGCTTTTCCCTC CTGGGCTATGATAATATAGCGAACGAAATACACGCCAAAATAAAP54 54 CTACGGAGGAAAAGTCTCACAAGCTTTTAACCAAGTTGAATCACGACGACAACGATAAAGAAATCCTCAACCA TCTAACACATGAAGTACAAAGTAGAAATGTGATCTTATTGGACAAACTAGAGGAGCTCAACAAGGAACTGGG CTGGATTAAAGACCGAAAATGAGGAACCATGAGCACTGGGCGTTTCCAGAAAAACTGCAACCAACGATGGG AAAATGATACCACACTACTATGGTCACCCCACATTGTGAAATTTCAAACCAAAAAAGATCAACCCCATAATTCC CCAGAGGGTTTTCCCAACAATTTTCCAACGGACTTGATAATGAGTCAGATCATTTGAGCATATTCATCTTACCC CTTATTCCGTGACAATTTACCTATTCCATTCAAAGCATACGGTATCCCGTGACCTTCTCATGGAGATCATTCTCC ACCGATACAGCATATACACAGATATACCCAACTAATATCAATTGGACCTTGATATGGTCGACCTTGATGGTCCC GTCCAACCTTAAAACTTAGTTTAATGCTATACTTTCGCCTTGAACCAAATCTGTCTCCCCCTCAATCATCTCTAT GCAAGAAGGTCAACACTGATTACGTGAGCAACAGCCAGCAATCGTTCGAGTCCCCGCCAAAAAAGGCGGAGT TACTGCTCCTTGTGACCACACCCCCTGAGACCACGTCCCTAAACGATCCTTGTCGGTTCCTTCGTCCAATTGGC AATTGCCACGCATACGTGAATCGTTATTGTTTCGCCTACCTTGCGTCATTCGTTCCAGAATGTTCGACATACTCC TCTAGAACATACCGTCACACCACCATCTTAAGTTATCTTCACGTGACCATGACGTACATTGTAGTTGACTACCC CATTCTCATCATTCCGATGCGGCCAAAAATCTCTATATAAAGACCGTATCCCCTAATATTCTCTTCTTGTTAAGA CATTAACTTAGTTAATTCACCAATTACTCACTTATAAACAAACAAAP55 55 TCCAGTGTAGCACTAAAATCTAATATCTTCGGCTTTATAUI 1 1 1 1 1 GTTCATCCGAAAGCTTACGAACAATTCTT TCTCCTGTTTTATTGTGGATATAGACAATTTCGTCAGTTTCTTGGAGAGAAGAGTTATTTCCGGTTTTGGCTGG CCCTATAAACGGGTTCTTGGATTTGGATCTAGTAATAAAAATGTCACTGTCATTCTCGGAGCTGAACTTTGTGT TGTACGAAGATGGGTTGTTCCACTGTTTTGCCAGCTCTTCATTGATGATTTTCTTAGTGGGTGTTCTTGGAGGT TCACGTTGCCTATAATCTTGACGTTCTTCTTCATCACTATCGATGCCATCAAAATTAAGCGTCCTTATTGCAGGC TTTTGTGATTTCAACTGCAATCC 1 1 L i ATCTCTTCATCAGAGCTTTCGAACTGAATACTATCACTCAAAACTGGC GACATTGCACATTTCCGCAAACCATTTCGGGAATCTATGCTAGCTCTTCTAGACGATAAAGAACGACCGGAAC CAATACGGGGTTGTGCAGGTGGGAATAAATATGTTGGTTTGGATTCTTGACGTGAAGAAGGTATTCTAGTCG ATGAAGTGGTTGATAAGGATATGGCGTCACTGAGTTGTTTTCTTTTCCTATGTTGCGGTGTTGGGTCAGGAGT TAATTGATTCACCTCCATAACTCTGGAATTTCTTGAATGTGGGGTTTTCAGATGGGCATCTTTCTTGACGGGGT TGTGAGTAACGGAGGAACCTGGTGTCTTGGGTGTGAACGGTGTTTGAGCCTGTACGCGGTTACTTCTGGGCG GAGTACTCGGAGTCATGAGAGCCATTGATTAGAAGGTGAATGAGGGAGTCACCACTCTAAGCAAACAAAATG AGGTCGAAGCAAAAAATAAAGTAAAGTAGCACTTCTGGCAGGTTAGATCAAAGAGTGACGGGAGATTTGAA GATGGCTGG i 1 i 1 i LC 1 1 AG 1 L i I GGAAGAGG I 1 I G I GTGGGTATCAGCGAATATTCCCCGATTAGGCAAATT AGTTGCATTGAAATTAACACGACATGGTGATTTGTGGTAACAAATATCTATTGGTGGTTGGTGTGTGGGTGTA ATAGTGGTCGTGTCATGATGATGGTGTTCAGGTGTTGTCATAGATCGGTCTTCAGTAAGAGAAGGAAGCTTGGTGACGATCACAGCTATGATGTAATAGAAATTGCTAAGCAATTGTGAGGTGTGATGTATTTTGCAGAGCAATTSequence SEQDescription iDNO. Sequence GTGCGGTACAACGGGGTGTTATTGTCTTCACAAGGCATTTATTGCGAATTTCGTAGTTGAAAGAATATTTTAG CACAGGGTGCTTGACCCCTATTGTTGCTCGCTAAACCATGATTGCTAAATGATGACATAGCAATCACTTTACTA AGATTGCTATAAGGACACCTTTCTTAGTATAAATGGACACTCTTTTCCCCTGCTAAACTTCTTTTATTTTTCACAC TTAAACAGTTACAAAACACAAACACAACTAGAAP56 56 CAAACATTTGCTCCCCCTAGTCTCCAGGGAAATGTAAAATATACTGCTAATAGAAAACAGTAAGACGCTCAGT TGTCAGGATAATTACGTTCGACTGTAGTAAAACAGGAATCTGTATTGTTAGAAAGAACGAGAGTTTTTTACGG CGCCGCCATATTGGGCCGTGTGAAAACAGCTTGAAACCCCACTACTTTCAAAGGTTCTGTTGCTATACACGAA CCATGTTTAACCAACCTCGC I I 1 1 GACTTGACTGAAGTCATCGGTTAACAATCAAGTACCCTAGTCTGTCTGAA TGCTCCTTTCCATATTCAGTAGGTGTTTCTTGCACTTTTGCATGCACTGCGGAAGAATTAGCCAATAGCGCGTT TCATATGCGCTTTTACCCCCTCTTTTGTCAAGCGCAAAATGCCTGTAAGATTTGGTGGGGGTGTGAGCCGTTA GCTGAAGTACAACAGGCTAATTCCCTGAAAAAACTGCAGCTCAGGGATTCCCACTATTTGGTATTCTGATATGI 1 I 1 1 CC 1 GA 1 A 1 GCA I CAAAAC 1 C 1 AA I C 1 AAAACC 1 GAA 1 C 1 CCGC 1 A 1 I 1 I I I 1 1 1 1 1 1 1 1 I GATGACCCCGT TTTCGTGACAAATTAATTTCCAACGGGGTCTTGTCCGGATAAGAGAATTTTGTTTGATTATCCGTTCGGATAAA TGGACGCCTGCTCCATA! 1 I 1 I CCGGTTATTACCCCACCTGGAAGTGCCCAGAATTTTCCGGGGATTACGGATA ATACGGTGGTCTGGATTAATTAATACGAGATCTCAGGGATTCCCACTATTTGGTATTCTGATATGTTTTTCCTG ATATGCATCAAAACTCTAATCTAAAACCTGAATCTCCGCTATTTTTTTTTTTTTTTGATGACCCCGTTTTCGTGAC AAATTAATTTCCAACGGGGTCTTGTCCGGATAAGAGAATTTTGTTTGATTATCCGTTCGGATAAATGGACGCCT GCTCCATA! 1 I 1 I CCGGTTATTACCCCACCTGGAAGTGCCCAGAATTTTCCGGGGATTACGGATAATACGGTG GTCTGGATTAATTAATACGCCAAGTCTTACATTTTGTTGCAGTCTCGTGCGAGTATGTGCAATAATAAACAAGA TGAGCCAATTTATTGGATTAGTTGCAGCTTGACCCCGCCATAGCTAGGCATAGCCAAGTGCTATGGGTGTTAG ATGATGCACTTGGATGCAGTGAGTTTTGGAGTATAAAAGATCCTrAAAATTCCACCCTrP57 57 CAAACATTTGCTCCCCCTAGTCTCCAGGGAAATGTAAAATATACTGCTAATAGAAAACAGTAAGACGCTCAGT TGTCAGGATAATTACGTTCGACTGTAGTAAAACAGGAATCTGTATTGTTAGAAAGAACGAGAGTTTTTTACGG CGCCGCCATATTGGGCCGTGTGAAAACAGCTTGAAACCCCACTACTTTCAAAGGTTCTGTTGCTATACACGAA CCATGTTTAACCAACCTCGCTTTTGACTTGACTGAAGTCATCGGTTAACAATCAAGTACCCTAGTCTGTCTGAA TGCTCCTTTCCATATTCAGTAGG 1 G 1 i 1 C I I GCAL M H GCATGCACTGCGGAAGAATTAGCCAATAGCGCGTT TCATATGCGCI 1 1 I ACCCCCTCTTTTGTCAAGCGCAAAATGCCTGTAAGATTTGGTGGGGGTGTGAGCCGTTA GCTGAAGTACAACAGGCTAATTCCCTGAAAAAACTGCAGATAGACTTCAAGATCTCAGGGATTCCCACTATTT GGTATTCTGATATG 1 i 1 i 1 CC 1 GA 1 A 1 GCA I CAAAAC 1 C 1 AA I C 1 AAAACC 1 GAA 1 L 1 CCGC 1 A 1 I I I I 1 i 1 1 1 1 1 TTTGATGACCCCGTTTTCGTGACAAATTAATTTCCAACGGGGTCTTGTCCGGATAAGAGAATTTTGTTTGATTA TCCGTTCGGATAAATGGACGCCTGCTCCATAI 1 1 1 1 CCGGTTATTACCCCACCTGGAAGTGCCCAGAATTTTCC GGGGATTACGGATAATACGGTGGTCTGGATTAATTAATACGCCAAGTCTTACATTTTGTTGCAGTCTCGTGCG AGTATGTGCAATAATAAACAAGATGAGCCAATTTATTGGATTAGTTGCAGCTTGACCCCGCCATAGCTAGGCA TAGCCAAGTGCTATGGGTGTTAGATGATGCACTTGGATGCAGTGAGTTTTGGAGTATAAAAGATCCTTAAAAT TCCACCCTTP58 58 AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACC 1 1 1 1 1 GCCATCCGACATCCACAGGTCCATTCTCACAC ATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCT TCTCCTCAACACCCAC 1 1 I I GCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAA TTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTT GTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGG GTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAA AAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAAC TTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCT TAGCGCAGTCTCTCTATCGCI 1 C 1 GAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTT TTGGATGATTATGCATTGTCTCCACACTGCTGATAGCCACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCrTTTTTTrTATCATCATTATTAGCTTACTTSequence SEQDescription iDNO. Sequence TCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAA AAACAACTAATTATTCGAAACGP59 59 TGGGTTGTATCCATTCACTATTTACTCTTTGTTTCATTTCTTGAATTATTTGGATACTACTCTGCTGGCAACTCTA CCAGTCTCAAACGCAGACCAGGTTCGCAATTTGATTAGAATGTTCGTGAGCTCTTACAATGAAAAGTCCATGT ACCTTGCGGCTAGTTGTGAATTAI 1 1 1 1 AGTTCCTTCTTTGTTGCTATCCTCTTTGAAGTCGATTATATTGCTGG AATGGTATAGGGCTCCCI 1 1 1 CATTTATCAGGCAATTAATCGTGGTATTCTCCGTGATCTCGTTTCTGAGATTAA GATATCAACAGAATGTTTACATGAAACAATTAGTTGATAGTTATGATTTGAAGATCAGTCAACTCTTATACCAT CCCCAACTTCCTCAAGGATTCAGGTTGGGATATTTACGATTTAAGAGTCTATTAACAAGCACGCTAGGATACTT AGAATTGGAAAAAAAGACCAGATAATGAGATTGAACTCGAAATTTAGGATCACCCATATGACGAAGAATTCA TTTAGATTATTGAAGGTGTTTTCATGTTTACCTCCATGAGACCATTTCTGTCACAGCAAATACAGGCAACGCTT TTCACCAGAGCTTGTTGGTACAAL i 1 1 1 CAGA 1 GACGCCAAA U LI LACGCGCL 1 CAC i 1 1 G 1 GCGGCGCTAAC AATAGGCCA I I I 1 1 1 1 G 1 ACC 1 CCCGGA I GG I 1 CAGC 1 CAA 1 CAC 1 CGA 1 I GAGAGG I I I I 1 GTTCCGCGATTTT TGTTCACCCCACACTTTTCTCGAAGGTTCTAGCAATCAAGATAAACACCGCAAAGAGAGCCGCAGGAACCATA TGTGGTACCACAAGTGGTCTTAAACAACTCTGGTAGAATTCGATGGAATTCGATGGAAGCCGATCGACTCCGA TCGAATTGAAGCAATTCGTATATATAAGGAGAACCTAGTTCCACCCCTTACTCGACCATTAGTTTACAAGACTA ACTTCACAGAAGCATAGAAATTAAACAAAGTTAAACATTP60 60 ACGAGCCATlTHCGATCCGTGTCAGTrCCAGACCAGCAGTTTAACTACGCAAATCCACAGGAATTTCTACATC ACAATACCAATGGTAATACCACGACGTCAAGGAATGGAAACGACGACTTGGAGGAAAACTTCGTCAACCTCT TGCGGAGTACCCGAGGCTAAGACAATAAGAAGAAAAAAAAAGAAAAGCGGTGGGGGAGGGATTATTAAAT AAGGATTATGTAACCCCAGGGTACCGTTCTATACATATTTAAGGATTATTTAGGACAATCGATGAAATCGGCA TCAAACTGGATGGGAGTATAGTGTCCGGATAATCGGATAAATCATCTTGCGAGGAGCCGCTTGGTTGGTTGG TGAGAGGAGTGAAATATGTGTCTCCTCACCCAAGAATCGCGATATCAGCACCCTGTGGGGGACACTATTGGC CTCCCTCCCAAACCTTCGATGTGGTAGTGCTTTATTATATTGATTACATTGATTACATAGCTAAACCCTGCCTGG TTGCAAGTTGAGCTCCGAATTCCAATATTAGTAAAATGCCTGCAAGATAACCTCGGTATGGCGTCCGACCCCG CTTAATTATTTTAACTCCTTTCCAACGAGGACTTCGTAAI 1 1 I 1 GATTAGGGAGTTGAGAAACGGGGGGTCTTG ATACCTCCTCGATTTCAGATCCCACCCCCTCTCAGTCCCAAGTGGGACCCCCCTCGGCCGTGAAATGCGCGCAC TTTAGTTTTTTTCGCATGTAAACGCCGGTGTCCGTCAATTAAAAGTCGCAGACTAGGGTGAACTTTACCATTTT TGTCGCACTCCGTCTCCTCGGAATAGGGGTGTAGTAATTCTGCAGTAGTGCAA H i l l ACCCCGCCAAGGGGG GGCGAAAAGAGACGACCTCATCACGCATTCTCCAGTCGCTCTCTACGCCTACAGCACCGACGTAGTTAACTTT CTCCCATATATAAAGCAATTGCCATTCCCCTGAAAACTTTAACCTCTGCI 1 1 1 I CI I GA! 1 1 1 1 CCTTGCCCAAAG AAAAGP61 61 GAATTCGGACAAATGTGCTGTTCCGGTAGCTTGTAGGAAGCGGCATCCGTAGGGCAATATACGACTATAGCT TCTAAAGCGTAGTACAATGAAATGTTCGAAGGAACAACAAACGGATTTG 1 1 1 1 1 CGTAGGCTCAACCCGTTGA GGTGTAACTCTTTAGCGAAAGGGTAAGATTGATTGTTCGAAGTAGGGCCTCAAAGGGAAAGAGAAAAAAAA AAATACACCGAAGAGTTACGTAAGCATATA 1 1 1 1 1 TACGTAAAGCATGATTGAATTTCAGCAGTATTGTTTAAC AAGGCTGATGTCGTGTGCCAATCAAAACAAAAGAGATTCGCATAATGCCATAATTGGGGTGTGTGGGCGCCC CCTAAAACGTCTTTCTCATCATCATCTGCAACCCCCATCGAACCTCATTAAATCACATGACTTGTGCGATCCTCG GTCAACTCGTTCCGTGCACCCATTCCACCCCGGGCTGACCAACGCAAGGTTCTCCGAGAGTCCGCTACCCCAG ATTTATATCAGCAACCAGTCACCTTTTTCCGGGCACGACTCTATATGCCCTGGAAAACCGGAGACGATGAGCC TGACTATAAAAGGTGACAGAACCCCCAACTCTGGTTAATCTCTTCAACAAATACTTTATTTTCTTTCAATTCAAA GAACACAGTATCAAGTATATCAAGAP62 62 AAAGTCAGCGGAATGTTTGAACAGGGTCCATCTGAGAAAGAGGAGCCAGCACAGGAATCGGTGGTAATAAA CGAACTCATGGAAAAAGCACAGTCCAGGCCTGAAAAGCCACCCAAAAGACTGGCAGAGTTGGAGGAAATCT GGGTGGATGGTTTGCAAAAGAAATACGGTAACGACTATGAAAGAATGAAATGGGATCGCAAACTGAACCCC ATGATGCTAACGCCCCGCAAGTTGGAGAAACTGTTTGAAAGGAAATTGCAGGTCGAAGATTACGACAGAAGA CATACAGCATGATTAGGTCACACGGCTTGTCG 1 1 1 1 I C 1 1 1 C 1 1 1 1 1 1 I 1 ACCCACGTAGAAGGATTATAGGGACTGAACTGACATGACATGTGACTGTAACAGATAGGTAAGTAAGGCACATAATTTAAGTGACAGTGTCAACAASequence SEQDescription iDNO. Sequence TGCCAATTT1T1T1TFTTTACAACAATGCCCACGTTGTTACCGATGCTCGCACGTAGATTCCTTTACTGGCCAAG AACTTGGCGCGAAATGTGATATCGATTGAATCTGATAAAAGAAACAAAAAAAAAAAAAATTTATCCGGTGTA GGGATGTGACTAAACGCCTCGTGTGACAACGTGGGTGGTATCTTTCCTTCGCCACGTGCGCGTGCAACCTATT GTATGGCTAAATTAAAGGGCCATGTCAAAGACCCTTAAACCCTTGATAATAGCAAAGACTGGGGAAGCAGGC CCTGAAAAGACAGAACAATCAGAATTCTCTAATTTCCTCCACCAGAGAGCCGTCCAATGGCATAGATTACCTC GTTAAACGAAATTCGAAAGCTCAATTTCCAAATACCCACTTTCCTTTTCGATCCTTATTGCTGCTGGACCTACTC TCCAAAG 1 L i 1 GGLAAGGLCACA 1 G 1 L 1 LAA 1 i i 1 1 AATTGAAAACCGTTAGAAAGTGAAGCCGACTTTAGGT ATAAATAGTAGGAACCCCAGCAGGCAGAGCTGATCACTTGTAAACCTTTCTCTGATACACACAAGCATCTATTT GAGTACTCATCTTATAATCATTCACAP63 63 GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGGAT ATGCCGCCTATAGGATAAAAACAGGAGAGGGTGAACCTTGCTTATGGCTACTAGATTGTTCTTGTACTCTGAA TTCTCATTATGGGAAACTAAACTAATCTCATCTGTGTGTTGCAGTACTATTGAATCGTTGTAGTATCTACCTGG AGGGCATTCCATGAATTAGTGAGATAACAGAGTTGGGTAACTAGAGAGAATAATAGACGTATGCATGATTAC TACACAACGGATGTCGCACTCTTTCCTTAGTTAAAACTATCATCCAATCACAAGATGCGGGCTGGAAAGACTT GCTCCCGAAGGATAATCTTCTGCTTCTATCTCCCTTCCTCATATGGTTTCGCAGGGCTCATGCCCCTTCTTCCTT CGAACTGCCCGATGAGGAAGTCCTTAGCCTATCAAAGAATTCGGGACCATCATCGAI 1 1 1 1 AGAGCCTTACCT GATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCTCCAACTTTGCTTGTTCATACAATTCTT GATATTCACAP64 64 TCCCATTACCGACATTTGGGCGCTATACGTGCATATGTTCATGTATGTATCTGTATTTAAAACACTTTTGTATTA H I CCTCATATATGTGTATAGGTTTATACGGATGATTTAATTATTACTTCACCACCCTTTATTTCAGGCTGATA TCTTAGCCTTGTTACTAGTTAGAAAAAGACAI 1 1 1 1 GCTGTCAGTCACTGTCAAGAGATTCTTTTGCTGGCATTT CTTCTAGAAGCAAAAAGAGCGATGCGTCTTTTCCGCTGAACCGTTCCAGCAAAAAAGACTACCAACGCAATAT GGATTGTCAGAATCATATAAAAGAGAAGCAAATAACTCCTTGTCTTGTATCAATTGCATTATAATATCTTCTTG TTAGTGCAATATCATATAGAAGTCATCGAAATAGATATTAAGAAAAACAAACTGP65 65 TCCi i I 1 1 ACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGTTCCGTTCGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGTG AGCGCTCTCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGT GCGAATATAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTC ACCTCACTATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTATT TACTTTACGAAA SSI 66 MFNLKTILISTLASIAVASS2 67 MQVKSIVNLLLACSLAVASS3 68 MQFNWNIKTVASILSALTLAQASS4 69 MAATTCFFFLFPFLLLLTLSRASS5 70 MLLQAFLFLLAGFAAKISASS6 71 MVAWWSLFLYGLQVAAPALASS7 72 MSFSSNVPQLFLLLVLLTNIVSGSS8 73 MNLYLITLLFASLCSASequence SEQDescription iDNO. SequenceSS9 74 MKSLILNIISVTLAITSTAASS10 75 MRFPSIFTAVLFAASSALASS11 76 MFSPILSLEIILALATLQSVFASS12 77 MSFRSLLALSGLVCSGLASS13 78 MSFRSLLALSGLVCSGLANVISKRSS14 79 MKLAYSLLLPLAGVSASS15 80 MTKPTQVLVRSVSILFFITLLHLVVASS16 81 MKLSTNLILAIAAASAVVSASS17 82 MKILSALLLLFTLAFASS18 83 MRPVLSLLLLLASSVLASS19 84 MFKSLCMLIGSCLLSSVLASS20 85 MSTLTLLAVLLSLQNSALASS21 86 MKLLNFLLSFVTLFGLLSGSVFASS22 87 M KVSTTKF LAVF LLVR LVCASS23 88 MYRNLIIATALTCGAYSSS24 89 MFLKSLLSFASILTLCKAWDLEDVQDAPKSS25 90 MLSILSALTLLGLSCASDLTPPIEVTGNKFFFSS26 91 MINLNSFLILTVTLLSPALALPKNVLEEQQASS27 92 MRNHLNDLVVLFLLLTVAAQAHLVTFHSTESS28 93 M KLSATLLLSVFTSI QSAYASS29 94 MRRRAIPLLLLLLLLLLLGSSALASS30 95 MRQVWFSWIVGLFLCFFNVSSASS31 96 MQVKSIVNLL LACSLAVARPLEHAHHQHDK RSS32 97 MKLSTNLILAIAAASAVVSAAPVAPAEEAANHLHKRSS33 98 MYRNLIIATALTCGAYSAYVPSEPWSTLTPDASLESALKDYSQTFGIAIKSLDADKIKRSS34 99 MFNLKTILISTLASIAVAAPVNTTTEDETAQJPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS LDKR SS35 100 MQVKSIVNLLLACSLAVAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGV SLDKR SS36 101 MQFNWNIKTVASILSALTLAQAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAA KEEGVSLDKR SS37 102 MAATTCFFFLFPFLLLLTLSRAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKE EGVSLDKR SS38 103 MLLQAFLFLLAGFAAKISAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLDKR SS39 104 MVAWWSLFLYGLQVAAPALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAK EEGVSLDKR SS40 105 MSFSSNVPQLFLLLVLLTNIVSGAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAK EEGVSLDKR SS41 106 MNLYLITLLFASLCSAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLD KR SS42 107 MKSLILNIISVTLAITSTAAAPVNTTTEDETAQiPAEAViGYLDLEGDFDVAVLPFSNSTNNGLLFiNTTIASiAAKEEGV SLDKR SS43 108 MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLDKRSequence SEQDescription IDNO. SequenceSS44 109 MFSPILSLEIILALATLQSVFAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEE GVSLDKR SS45 110 MSFRSLLALSGLVCSGLAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGV SLDKR SS46 111 MSFRSLLALSGLVCSGLANVISKRAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIA AKEEGVSLDKR SS47 112 MKLAYSLLLPLAGVSAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSL DKR SS48 113 MTKPTQVLVRSVSILFFITLLHLVVAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASI AAKEEGVSLDKR SS49 114 MKLSTNLILAIAAASAVVSAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEE GVSLDKR SS50 115 MKILSALLLLFTLAFAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLD KR SS51 116 MRPVLSLLLLLASSVLAAPVNTTTEDETAQIPAEAViGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASiAAKEEGVSL DKR SS52 117 MFKSLCMLIGSCLLSSVLAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLDKR SS53 118 MSTLTLLAVLLSLQNSALAAPVNTTTEDETAQ. IPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLDKR SS54 119 MKLLNFLLSFVTLFGLLSGSVFAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAK EEGVSLDKR SS55 120 MKVSTTKFLAVFLLVRLVCAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEE GVSLDKR SS56 121 MYRNLHATALTCGAYSAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS LDKR SS57 122 MFLKSLLSFASILTLCKAWDLEDVQDAPKAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINT TIASIAAKEEGVSLDKR SS58 123 MLSILSALTLLGLSCASDLTPPIEVTGNKFFFAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFIN TTIASIAAKEEGVSLDKR SS59 124 MINLNSFLILTVTLLSPALALPKNVLEEQQAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFIN TTIASIAAKEEGVSLDKR SS60 125 MRNHLNDLVVLFLLLTVAAQAHLVTFHSTEAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFI NTTIASIAAKEEGVSLDKR SS61 126 MKLSATLLLSVFTSIQSAYAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLDKR SS62 127 MRRRAIPLLLLLLLLLLLGSSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAK EEGVSLDKR SS63 128 MRQVWFSWIVGLFLCFFNVSSAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIA AKEEGVSLDKR SS64 129 MFNLKTILISTLASIAVAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS65 130 MQVKSIVNLLLACSLAVAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS66 131 MQFNWNIKTVASILSALTLAQAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS67 132 MAATTCFFFLFPFLLLLTLSRAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS68 133 MLLQAFLFLLAGFAAKISAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS69 134 MVAWWSLFLYGLQVAAPALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS70 135 MSFSSNVPQLFLLLVLLTNIVSGAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSequence SEQDescription iDNO. SequenceSS71 136 MNLYLITLLFASLCSAAPVNTTTEDETAQ. IPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS72 137 MKSLILNIISVTLAITSTAAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS73 138 MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS74 139 MFSPILSLEHLALATLQSVFAAPVNTTTEDETAQIPAEAViGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS75 140 MSFRSLLALSGLVCSGLAAPVNTTTEDETAQiPAEAViGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS76 141 MSFRSLLALSGLVCSGLANVISKRAPVNTTTEDETAQiPAEAViGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS77 142 MKLAYSLLLPLAGVSAAPVNTTTEDETAQiPAEAViGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS78 143 MTKPTQVLVRSVSILFFITLLHLVVAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS79 144 MKLSTNLILAIAAASAVVSAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS80 145 MKILSALLLLFTLAFAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS81 146 MRPVLSLLLLLASSVLAAPVNTTTEDETAQIPAEAViGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS82 147 MFKSLCMLIGSCLLSSVLAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS83 148 MSTLTLLAVLLSLQNSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASiAAKEEGVSLDKR SS84 149 MKLLNFLLSFVTLFGLLSGSVFAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS85 150 MKVSTTKFLAVFLLVRLVCAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS86 151 MYRNLIIATALTCGAYSAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKRSS87 152 MFLKSLLSFASILTLCKAWDLEDVQDAPKAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSL DKR SS88 153 MLSILSALTLLGLSCASDLTPPIEVTGNKFFFAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVS LDKR SS89 154 MINLNSFLILTVTLLSPALALPKNVLEEQQAAPVNTTTEDETAQiPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVS LDKR SS90 155 MRNHLNDLVVLFLLLTVAAQAHLVTFHSTEAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGV SLDKR SS91 156 MKLSATLLLSVFTSIQSAYAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS92 157 MRRRAIPLLLLLLLLLLLGSSALAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS93 158 MRQVWFSWIVGLFLCFFNVSSAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS94 159 MFNLKTILISTLASIAVAAPVNTTTEDETAQ. IPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS LEKR SS95 160 MQVKSIVNLLLACSLAVAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLEKR SS96 161 MQFNWNIKTVASILSALTLAQAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAA KEEGVSLEKR SS97 162 MAATTCFFFLFPFLLLLTLSRAAPVNTTTEDETAQiPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKE EGVSLEKR SS98 163 MLLQAFLFLLAGFAAKISAAPVNTTTEDETAQ. IPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLEKR SS99 164 MVAWWSLFLYGLQVAAPALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFiNTTIASiAAK EEGVSLEKR SS100 165 MSFSSNVPQLFLLLVLLTNIVSGAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAA KEEGVSLEKR SS101 166 MNLYLITLLFASLCSAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLE KR SS102 167 MKSLILNIISVTLAITSTAAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRSequence SEQDescription iDNO. SequenceSS103 168 MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLEKR SS104 169 MFSPILSLEIILALATLQSVFAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEE GVSLEKR SS105 170 MSFRSLLALSGLVCSGLAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGV SLEKR SS106 171 MSFRSLLALSGLVCSGLANVISKRAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIA AKEEGVSLEKR SS107 172 MKLAYSLLLPLAGVSAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSL EKR SS108 173 MTKPTQVLVRSVSILFFITLLHLVVAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASI AAKEEGVSLEKR SS109 174 MKLSTNLILAIAAASAVVSAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEE GVSLEKR SS110 175 MKILSALLLLFTLAFAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLE KR SS111 176 MRPVLSLLLLLASSVLAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSL EKR SS112 177 MFKSLCMLIGSCLLSSVLAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLEKR SS113 178 MSTLTLLAVLLSLQNSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLEKR SSI 14 179 MKLLNFLLSFVTLFGLLSGSVFAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAK EEGVSLEKR SS115 180 MKVSTTKFLAVFLLVRLVCAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEE GVSLEKR SS116 181 MYRNLIIATALTCGAYSAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVS LEKR SS117 182 MFLKSLLSFASILTLCKAWDLEDVQDAPKAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINT TIASIAAKEEGVSLEKR SS118 183 MLSILSALTLLGLSCASDLTPPIEVTGNKFFFAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFIN TTIASIAAKEEGVSLEKR SS119 184 MINLNSFLILTVTLLSPALALPKNVLEEQQAAPVNTTTEDETAQiPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFIN TTIASIAAKEEGVSLEKR SS120 185 MRNHLNDLVVLFLLLTVAAQAHLVTFHSTEAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFI NTTIASIAAKEEGVSLEKR SS121 186 MKLSATLLLSVFTSIQSAYAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLEKR SS122 187 MRRRAIPLLLLLLLLLLLGSSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAK EEGVSLEKR SS123 188 MRQVWFSWIVGLFLCFFNVSSAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTiASIA AKEEGVSLEKR SS17_L8F 189 MKILSALFLLFTLAFASS17_L8N 190 MKILSALNLLFTLAFASS17_L8T 191 MKILSALTLLFTLAFASS17__L8V 192 MKILSALVLLFTLAFASS17_L9C 193 MKILSALLCLFTLAFASequence SEQDescription iDNO. SequenceSS17J.9F 194 MKILSALLFLFTLAFASS17_L9I 195 MKILSALLILFTLAFASS17„L9M 196 MKILSALLMLFTLAFASS17J.10F 197 MKILSALLLFFTLAFASS17__L10i 198 MKILSALLLIFTLAFASS17_L10M 199 MKILSALLLMFTLAFASS17_F15K 200 MKILSALLLLFTLAKASSI 24 201 M K F AISTLLI 1 LQAAAVF AASS125 202 MKSQLIFMALASLVASSS126 203 MKFAISTLLIILQAAAVFAAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEG VSLDKR SS127 204 MKSQLIFMALASLVASAPVNTTTEDETAQiPAEAVIGYLDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSL DKR SS128 205 MKFAISTLLIILQAAAVFAAAPVNTTTEDETAQIPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR SS129 206 MKSQLIFMALASLVASAPVNTTTEDETAQiPAEAVIGYLDLEGDFDVAVLPFLASIAAKEEGVSLDKR0ST1-AMF 207 ATGAGGCAGGTTTGGTTCTCTTGGATTGTGGGATTGTTCCTATGTTTTTTCAACGTCTCTTCTGCTGCTCCAGTC Coding AACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTTAGATTTAGAAGGG Sequence GATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCC AGCATTGCTGCTAAAGAAGAAGGGGTATCTTTGGATAAAAGABrazzein 208 GACAAATGCAAGAAAGTCTACGAGAACTACCCCGTATCGAAGTGTCAACTAGCCAATCAGTGTAACTACGATT Coding GTAAGCTCGATAAACATGCTCGCTCCGGAGAATGCTTCTATGACGAGAAGCGGAATCTGCAGTGTATTTGCG sequence ATTATTGCGAATATTGADHAS1 209 ACGGGAAGTCTTTACAGTTTTAGTTAGGAGCCCTTATATATGACAGTAATGCTAGTACGTTTTGTTTTGTTTAA Transcriptio TTAATAACTTAGTTTATGTTAGCCTAGTATAGACTCCATCAATTTTTTTTGTTATTACGTAAGCCGCGATGATAA n TATCTGATGAAAAATTCCTATCAGAAAATAATTTATCAAAAGTTTCATGCGATATGAGACTAAGTAGAATAGG Terminator GACTCCCAAAGTGTCAGTCACAAGGGTCATTCCCGTTCGTAATGTGGTGATAGCGAGGAGAAAACCTGTCAG Sequence AGCAAGTAACACCGACGCAAAGACATGGCTAATGAAAGAAGAGCAGAGAAGAATAAGACAGAAGGAGCAG GAGATGAAACAAAGGCTAGAGGAACTAGAAAGGTTCAAAACAAAAGTACAGAAATCATATATAAGGAAAGA GGATAGGCATTTGGCACAAGAGATAGAAAAGGATCTTGACATAATCACTGATGATTACAATTTGG AOX1 210 TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATAC i i i 1 1 1 ATTTGTAACCTA Transcriptio TATAGTATAGGA 1 1 1 i 1 i 1 1 G 1 CATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCAG n ATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATG 1 i i i i CTTGGTATTTCCCACTCCTCTTC Terminator AGAGTACAGAAGATTAAGTGAGACCTTCGTTTGTGCSequenceMoneilin 211 GGTGAATGGGAAATAATCGATATAGGGCCGTTCACCCAGAACTTGGGAAAGTTTGCGGTGGACGAAGAGAA coding TAAAATCGGGCAATACGGACGACTGACATTCAATAAAGTAATTCGCCCTTGTATGAAAAAAACGATATATGAA sequence GAGAATGGCTTCCGCGAAATTAAGGGCTACGAGTATCAACTTTACGTCTATGCTTCAGATAAGCTATTTCGTG CAGACATCAGCGAGGACTACAAAACTCGGGGAAGGAAGTTACTCAGATTTAACGGTCCGGTTCCACCCCCCT GAThaumatin I 212 ATFEiVNRCSYTVWAAASKGDAALDAGGRQLNSGESWTINVEPGTKGGKiWARTDCYFDDSGRGICRTGDCGGL sequence LQCKRFGRPPTTLAEFSLNQYGKDYIDISNIKGFNVPMDFSPTTRGCRGVRCAADIVGQCPAKLKAPGGGCNDACT VFQTSEYCCTTGKCGPTEYSRFFKRLCPDAFSYVLDKPTTVTCPGSSNYRVTFCPTAThaumatin II 213 ATFEIVNRCSYTVWAAASKGDAALDAGGRQLNSGESWTINVEPGTKGGKIWARTDCYFDDSGRGICRTGDCGGL sequence LQCKRFGRPPTTLAEFSLNQYGKDYIDiSNiKGFNVPMDFSPTTRGCRGVRCAADIVGQCPAKLKAPGGGCNDACT VFQTSEYCCTTGKCGPTEYSRFFKRLCPDAFSYVLDKPTTVTCPGSSNYRVTFCPTABrazzein-54 214 XDKCKKVYENYPVSKCQLANQCNYDCKLDKHARSGECFYDEKRNLQCiCDYCEYBrazzein-53 215 DKCKKVYENYPVSKCQLANQCNYDCKLDKHARSGECFYDEKRNLQCICDYCEYSequence SEQDescription iDNO. SequenceMoneilin 216 FREIKGYEYQLYVYASDKLFRADISEDYKTRGRKLLRFNGPVPPPchain AMoneilin 217 GEWEIIDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPCMKKTIYEENchain BSingle-chain 229 GEWEHDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPCMKKTIYEENGFREIKGYEYQLYVYASDKLFRADISEDY moneilin KTRGRKLLRFNGPVPPPMiraculin 218 DSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVV RVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFV TIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNKTVYFMabinlin-1 219 EPLCRRQFQQHQHLRACQRYIRRRAQRGGLVDchain AMabinlin-1 220 EQRGPALRLCCNQLRQVNKPCVCPVLRQAAHQQLYQGQIEGPRQVRQLFRAARNLPNICKIPAVGRCQFTRW chain BMabinlin-2 221 QLWRCQRQFLQHQRLRACQRFIHRRAQFGGQPDchain AMabinlin-2 222 QPRRPALRQCCNQLRQVDRPCVCPVLRQAAQQVLQRQ. IIQGPQ. QLRRLFDAARNLPNICNIPNIGACPFRAW chain BMabinlin-3 223 EPLCRRQFQQHQHLRACQRYLRRRAQRGGLADchain AMabiniin-3 224 EQRGPALRLCCNQLRQVNKPCVCPVLRQAAHQQLYQGQIEGPRQVRRLFRAARNLPNICKIPAVGRCQFTRW chain B orMabinlin-4chain BMabinlin-4 225 EPLCRRQFQQHQHLRACQRYLRRRAQRGchain ACurculin 1 226 DNVLLSGQTLHADHSLQAGAYTLTIQNKCNLVKYQNGRQIWASNTDRRGSGCRLTLLSDGNLVIYDHNNNDVW GSACWGDNGKYALVLQKDGRFVIYGPVLWSLGPNGCRRVNGCurculin 2 227 DSVLLSGQTLYAGHSLTSGSYTLTIQ. NNCNLVKYQHGRQIWASDTDGQGSQCRLTLRSDGNLIIYDDNNMVVWG SDCWGNNGTYALVLQQDGLFVIYGPVLWPLGLNGCRSLNMycoduScei 228 MPDLSSFITIKNNSNHVFTRTAIYSKYAAVQWSPEPQLSISPGKWDLFILKDILSIRGTSGYVQYRVGDGn PGWVRVTFSSLVGADEVAEWSSGDLPDGFVLQKPVRTGSRPLQATFEATKQSingle-chain 229 GEWEHDIGPFTQNLGKFAVDEENKIGQYGRLTFNKVIRPCMKKTIYEENGFREIKGYEYQLYVYASDKLFRADISEDY moneilin KTRGRKLLRFNGPVPPPP66 230 TCCTTTTTACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGCGACCGGTGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGT GAGCGCTCTCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAATATAGCGCACCCCCAATGATCACACCAACAATTGGTCCCCAATCTCTAATATTCACAATTCACCTCACSequence SEQDescription iDNO. Sequence TATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTATTTACTTTA CGAAAP67 231 TCCI 1 1 1 I ACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGCGACCGGTGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGT GAGCGCTTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAATA TAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCACT ATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTATTTACTTTAC GAAAP68 232 TCCI 1 1 1 1 ACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCTTTCCATTTGGGAGAATCTGCATGTCGACTACATAAAGCGACC GGTGTCCGAAAAGATCTGTGTAb 1 i I I CAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGTGAGCGCT CTCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAAT ATAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCAC TATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCl 1 i 1 ATCTTATTTACTTTA CGAAAP69 233 TCCI 1 1 1 1 ACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTCCCACCCCC CTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAAAGCGACC GGTGTCCGAAAAGATCTGTGTAb 1 i 1 1 CAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGTGAGCGCT CTCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAAT ATAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCAC TATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCI 1 i 1 ATCTTATTTACTTTACGAAASequence SEQDescription iDNO. SequenceP70 234 TCC’frfTTACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGCGACCGGTGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGT GAGCGCTCTCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGG TGCGAATATAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATT CACCTCACTATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTAT TTACTTTACGAAAP71 235 TCCTm’TACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGCGACCGGTGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGT GAGCGCTCTCCGGGGTGCGAATTCGTGCCCAATTCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAAT ATAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCAC TATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTATTTACTTTA CGAAAP72 236 TCCTm’TACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGCGACCGGTGTCCGAAAAGATCTGTGTAGTTTTCATGCTCCCCCCGCTGTTTGAAAACGGGGGTGAGCGCTC TCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAATA TAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCACT ATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTATTTACTTTAC GAAAP73 237 TCCTm’TACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGTGTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAAC 1 1 H C 1 CGCATCAATTGATCCTGTTACCAATATTGCATSequence SEQDescription iDNO. Sequence GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTCCCACCCCCCTTTCC AACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAAAGGTCCGAAAAGA TCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGTGAGCGCTCTCCGGGGTGCGAA TTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAATATAGCGCACCCCCA ATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCACTATAAATACCCCTG TCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTrATTTACTTTACGAAAP74 238 TCCTTTTTACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAATTGTCCAATCTCGTTTTCCATTTGGGAGAATCTGCATGTCGACTACATAA AGGTCCGAAAAGATCTGTGTAGTTTTCAACATTTTGTGCTCCCCCCGCTGTTTGAAAACGGGGGTGAGCGCTC TCCGGGGTGCGAATTCGTGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAATA TAGCGCACCCCCAATGATCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCACT ATAAATACCCCTGTCCTGCTCCCAAATTCTTTTTTCCTTCTTCCATCAGCTACTAGCTTTTATCTTATTTACTTTAC GAAAP75 239 TCCTTTTTACCACCCAAGTGCGAGTGAAACACCCCATGGCTGCTCTCCGATTGCCCCTCTACAGGCATAAGGGT GTGACTTTGTGGGCTTGAATTTTACACCCCCTCCAACTTTTCTCGCATCAATTGATCCTGTTACCAATATTGCAT GCCCGGAGGAGACTTGCCCCCTAATTTCGCGGCGTCGTCCCGGATCGCAGGGTGAGACTGTAGAGACCCCAC ATAGTGACAATGATTATGTAAGAAGAGGGGGGTGATTCGGCCGGCTATCGAACTCTAACAACTAGGGGGGT GAACAATGCCCAGCAGTCCTCCCCACTCTTTGACAAATCAGTATCACCGATTAACACCCCAAATCTTATTCTCA ACGGTCCCTCATCCTTGCACCCCTCTTTGGACAAATGGCAGTTAGCATTGGTGCACTGACTGACTGCCCAACCT TAAACCCAAATTTCTTAGAAGGGGCCCATCTAGTTAGCGAGGGGTGAAAAATTCCTCCATCGGAGATGTATTG ACCGTAAGTTGCTGCTTAAAAAAAATCAGTTCAGATAGCGAGACTTTTTTGATTTCGCAACGGGAGTGCCTGT TCCATTCGATTGCAATTCTCACCCCTTCTGCCCAGTCCTGCCAATTGCCCATGAATCTGCTAATTTCGTTGATTC CCACCCCCCTTTCCAACTCCACAAACCCCGCTGTTTGAAAACGGGGGTGAGCGCTCTCCGGGGTGCGAATTCG TGCCCAATTCCTTTCACCCTGCCTATTGTAGACGTCAACCCGCATCTGGTGCGAATATAGCGCACCCCCAATGA TCACACCAACAATTGGTCCACCCCTCCCCAATCTCTAATATTCACAATTCACCTCACTATAAATACCCCTGTCCT GCTCCCAAA 1 1 C 1 1 1 1 1 1 LC 1 i L 1 1 CCA 1 CAGC 1 AC 1 AGC 1 1 H ATCTTATTTACTTTACGAAATX1 240 MMPEEQVTSPQRKHQKSKAKTIRAPGSSIERVAQACDRCRSKKTRCDGKRPQCSQCAAVGFECKISDKLSRRAFP RGYTETLEERiRELEFENKKLHKLIDLKNEQVEiKNRiDQESTLTNENLTLLNKEQEVSHSGNIHHHADGEPCNCANS VSARPVSIAGSVDIDTTDLSDEDDSLYSAASYNAKYHQTGTSGPEMVRLSQ. RYSSGNFNDPLSFEQSNAPGAAAAI SIQNKMRTQTFVNLANLVAMSIPRTTEETLFIASLLAKICNVHGFQSKAPILTAKSIALLKDKYNYGNDEVFANITLKN VNFNKLTSQQSQQFFQSLNLPNQVNLDLFITrFFNTWNNFIPIINRHIFMSSYlKFNKSRETMFTDNSMFGNEKFGE ILLLiTTMVMLSQERNNNREAVPSSSYKKDSTPHPHRPDASSQSNVEILQYYDHLIHEFIKSNISDDCSLPTLESLSLQLLYCLAIGDLTTSYELRGKIITMGQQLRLHRCPSAVLGTNGSKVSQMQQGERRILFWCIYILDTFSALILGVPRLLKDYESequence SEQDescription iDNO. Sequence IECALPFSNESNNANVKGSIENTTNTVIINNIKLSLAGKVSDCALAVMRYSKVLGNILDSIFQRSSINNPSVVSKSTNIT EETCLLHEHALDLWRRELSPHINVDLDKSPGGVEYERLSDNQLTILFLYYHAKILIYLPLMANESSQSRSSASYINIQQS TTSILAIANTLATKERNFYFLPLPVNLSREKVRLAFLSAKGSLEYARGGALFQESKILLASVINELKIETSIGMLGCLSVPC MEAVDNAMEQIMALPGKVSSVNGSNSEMKRSSSKRKSSPLRQDISGDERKSHNIEVSDSRTPSVQSSLYPQPNQ MHHPNIIKSENNEQMIPENDTPGAINDIFTSHSPPGTVTSMKEEDLPIKVPILLQTQQRQIYNNNPNHSLFSQQPG TQVLSGQQMPGPSSTDQQFKRITTPDGLDSLMMQDFGVDASLGLPMLDFDFNFDFENVQNNYSQSNVSPPNSE SVPSSIQGTHSNDPKDSQVSAGSLFGL TX2 241 MKENQASNKFNLIKNPITGKPRISQACDRCRIKKIKCDGTLPSCTNCSKIGFVCKISDRLTRSSFPKGYTKNLEQKLID MELDRNRLMLELNRIKKEGFDGTNNNIAMASSVSSSENLKSDDSSECQSVTVSLSSTSGPSLSPEPKQDDFRFRVG MDGSFVLNQFLQSPLMDYIKSLNVLQ. FNGCANFDQSFNDDPLVLNKYHMNLNRFLNLIFYKLLLPLIHRNSNTLNE KFAEDNNSLDSLIWKFFTNYNKLIPILEFDSFYKDYLQFIHKYYSNNQVFVDGFRKYFEFSEFEQCFIVKLILILKFTLPVI HDTSVPSEIYRLISMDSLQRLFGNIDFLKPSTDKVSILLLVLHYMVLYESPKSLLDTQDEAQKYDEFIGNLLSTAVHHIT SLRLHIDPRKLQFPRPLPSNGNRLRIKLSWCYKLISKLFRVIYNIDNDSLYSLDDSHLPELQSISILHEELDVTIQFNNLLN LIPNNFHSLRDKQSLSKIKTQLLEWHKNFNTEFVEHFNLNDTDSDELSAEKINVLRSKLISLNRLNCYNSYFQLVIELQ LKENLDSVVSGlFGLSNEMLIDNKSSTELLNTLQQTPilHQSSILVSLCYRIQTGNLQDEICSILVNNYEKLLQCNDAGL PIKILPQLVHYFKGKISTNLSNSAAHEDLMNMFTLNDNLSI 1 1 1 DLDSFIIPPKRKQDQ. TLPIGTKRSKSASTSSVISSD DCSLFSNSLSVPTTFSGSSISVGMDNPPSSLFGSYKRPSSIVKQEPTINPRSNGTNTDSNLFDTFNDSIKGSLNNGLKK LKDIRCNSVVERSHSSQRNDFLMDQEDSITKETINFSELFTCGTPTASQSIDRSPKSLLLNDLAIAPDTLVIKPDAEDLDRLKNKIRSVKSTVHX can be glutamine or pyroglutamate.EXAMPLES

[0107] Various aspects of the disclosure are further illustrated by the following non-limiting examples.Example 1: Promoter Screen for Brazzein Expression

[0108] Several promoters were tested for their abilities to promote brazzein expression.Construct Assembly

[0109] DNA fragments were de novo s...

Claims

WHAT IS CLAIMED IS:

1. An expression cassette comprising a promoter operably linked to a coding sequence of a heterologous protein, wherein the promoter comprises a first nucleotide sequence selected from any one of SEQ ID NOs: 232-238, and wherein the heterologous protein is operably linked to a signal peptide.

2. The expression cassette of claim 1, wherein the signal peptide comprises an amino acid sequence selected from the group consisting of SEQ, ID NOs: 66-71, 79, 81, 82, 85, 97, 100, 106, 107, 109, 113-116, 119, 120, 122, 125, 127, 134, 144, 145, 149, 150, 155, 158, 159, 166, 174, and 203.

3. The expression cassette of claim 1 or claim 2, wherein the heterologous protein is selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

4. A system comprising the expression cassette of any one of claims 1-3, and a second protein comprising the amino acid sequence of SEQ ID NO: 240 or 241.

5. The system of claim 4, wherein the coding sequence of the second protein is comprised within a second expression cassette and operably linked to a second promoter.

6. The system of claim 5, wherein the second promoter comprises a second nucleotide sequence selected from any one of SEQ ID NOs: 7, 33, and 65.

7. A eukaryotic cell comprising the expression cassette of any one of claims 1-3 or the system of any one of claims 4-6.

8. The cell of claim 7, wherein the cell a yeast cell or a filamentous fungal cell.

9. The cell of claim 7, wherein the cell is a Komagataella sp., optionally Komagataella phaffii.

10. A promoter comprising a first nucleotide sequence comprising a first nucleotide sequence set forth in any one of SEQ ID NOs:3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, and 235-239.

11. An expression cassette comprising a promoter operably linked to a coding sequence of a heterologous protein, wherein the promoter comprises a first nucleotide sequence selected from any one of SEQ ID NOs: 3-8, 10-13, 18, 26-32, 34, 56, 58, 65, 230-233, and 235-239.

12. The expression cassette of claim 11, further comprising a second nucleotide sequence encoding a signal peptide linked to the coding sequence of the heterologous protein.

13. The expression cassette of claim 11 or claim 12, wherein the heterologous protein comprises a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

14. An expression cassette comprising a promoter operably linked to a coding sequence of a heterologous protein that is a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, mycodulcein or a functional fragment thereof, wherein the promoter comprises a first nucleotide sequence selected from any one of SEQ ID NOs: 1-34, 65, and 232-238.

15. The expression cassette of claim 14, further comprising a second nucleotide sequence encoding a signal peptide linked to the coding sequence of the sweet protein.

16. The expression cassette of any of claims 11-15, wherein the first nucleotide sequence is selected from the group consisting of SEQ ID NOs: 3, 4, 5, 6, 7, 8, 9, 18, 19, 21, 25, 33, 34, 65, 232, 233, 234, 235, 236, 237, and 238.

17. The expression cassette of any of claims 12-16, wherein the second nucleotide sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 66, 81, 82, 97, 144, 145, 149, 150, 155, 159, 166, 174, and 189-200.

18. The expression cassette of claim 17, wherein the amino acid sequence is selected from the group consisting of SEQ ID NOs: 81, 82, 97, 144, 155, 190, and 195.

19. The expression cassette of claim 16 or claim 17, wherein the heterologous protein comprises brazzein, brazzein 53, brazzein-54 or a functional fragment thereof.

20. The expression cassette of any of claims 12-16, wherein the second nucleotide sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 100, 106, 107, 109, 113-116, 119, 120, 122, 125, 127, 149, 155, 158, and 203.

21. The expression cassette of claim 20, wherein the amino acid sequence is selected from the group consisting of SEQ ID NOs: 100, 109, 115, 119, 120, 149, and 158.

22. The expression cassette of claim 20 or claim 21, wherein the heterologous protein comprises monellin or a functional fragment thereof.

23. The expression cassette of any of claims 12-16, wherein the second nucleotide sequence encodes an amino acid sequence selected from the group consisting of SEQ ID NOs: 67-71, 79, 81, 85, 116, 134, and 150.

24. The expression cassette of claim 23, wherein the amino acid sequence is selected from the group consisting of SEQ ID NOs: 69, 79, 81, and 85.

25. The expression cassette of claim 23 or claim 24, wherein the heterologous protein comprises thaumatin, thaumatin I, thaumatin II or a functional fragment thereof.

26. A signal peptide, comprising the amino acid sequence set forth in any one of SEQ ID NOs: 99- 107, 109-158, or 203-206.

27. A protein coding sequence comprising a nucleic acid encoding the signal peptide of claim 26 or a signal peptide with an amino acid sequence set forth in any one of SEQ ID NOS: 67, 75, 77, 81, or 88 fused in frame to a coding sequence for a heterologous protein.

28. The protein coding sequence of claim 27, wherein the signal peptide is 5' to the heterologous protein.

29. The protein coding sequence of claim 27, wherein the signal peptide is 3' to the heterologous protein.

30. The protein coding sequence of any one of claims 27-29, wherein the heterologous protein is a sweet protein selected from the group consisting of brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, mycodulcein or a functional fragment thereof.

31. A protein coding sequence comprising a nucleic acid encoding a signal peptide comprising the amino acid sequence set forth in any one of SEQ ID NOS: 66-206 fused in frame to a coding sequence for a heterologous protein that is a sweet protein selected from the group consisting of a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

32. An expression cassette comprising the protein coding sequence of any one of claims 27-31.

33. The expression cassette of claim 32, further comprising a promoter, wherein the promoter comprises a first nucleotide sequence set forth in any one of SEQ ID NOS: 1-34, 65, or 232-238.

34. A vector comprising the promoter of claim 10, the expression cassette of any one of claims 1-3, 11-25, 32, or 33, the signal peptide of claim 26, or the protein coding sequence of any one of claims 27-31.

35. A cell comprising the promoter of claim 10, the expression cassette of any one of claims 1-3, 11- 25, 32, or 33, the signal peptide of claim 26, or the protein coding sequence of any one of claims 27-31.

36. The cell of claim 35, wherein the cell is a eukaryotic cell.

37. The cell of claim 35, wherein the cell is a yeast cell or a filamentous fungal cell.

38. The cell of claim 35, wherein the cell is Komagataella sp., optionally Komagataella phaffii.

39. A method of producing a heterologous protein, the method comprising inserting the expression cassette of any one of claims 1-3, 11-25, 32, or 33 into an expression system whereby the heterologous protein is produced.

40. The method of claim 39, further comprising the co-expressing or overexpressing of a transcription factor.

41. The method of claim 40, wherein the amino acid sequence of the transcription factor is SEQ ID NO: 240 or SEQ ID NO: 241.

42. The method of any one of claims 39-41, wherein the expression system is a cell.

43. The method of claim 42, wherein the expression system is a eukaryotic cell.

44. The method of claim 42, wherein the cell is a yeast cell or a filamentous fungal cell.

45. The method of claim 42, wherein the cell is Komagataella sp., optionally Komagataella phaffii.

46. The method of any of claims 39-45, wherein the heterologous protein is secreted into a culture medium.

47. The method of claim 46, further comprising collecting the culture medium.

48. The method of claim 47, further comprising isolating the heterologous protein from the culture medium.

49. The method of any one of claims 39-48, wherein the heterologous protein is a sweet protein selected from the group consisting of a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

50. An expression vector comprising any one of the promoter-signal sequence combinations set forth in Table 11, optionally, wherein the expression vector comprises a heterologous protein, optionally wherein the heterologous protein is selected from the group consisting of a brazzein, monellin, miraculin, mabinlin, curculin, thaumatin, or mycodulcein protein or a functional fragment thereof.

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