Composition for enhancing immunity

Abstract

The present invention relates to a composition for enhancing immunity prepared by mixing Angelica gigas, Cnidium officinale, and Paeonia japonica in a weight ratio of 1-10:1-10:1-10. The present invention also relates to a method for preparing the composition. The composition of the present invention is characterized by having an excellent immunity enhancing effect, being simple to prepare, and having high safety. The preparation method of the present invention has both novelty and efficiency, and has high utilization of active ingredients, thereby being able to also contribute to the efficient utilization of medicinal resources.

Description

Composition for boosting immunity

[0001] The present invention relates to a composition for enhancing immunity and a method for preparing the same.

[0002] Immunity is the human body's self-defense mechanism, referring to the body's ability to recognize and eliminate external invaders (viruses, bacteria, etc.), process aging, damaged, dead, or degenerated cells, and recognize and respond to mutated cells and virus-infected cells. In other words, immunity can be described as a physiological response and self-defense mechanism of the human body aimed at recognizing and eliminating "foreign substances." Human immune capacity is broadly divided into two types: specific immunity and non-specific immunity.

[0003] The human immune system consists of three lines of defense. The first line of defense is the skin and mucous membranes, which can block most pathogens at this stage. The second line of defense consists of antimicrobial substances in body fluids and various phagocytes, which eliminate pathogens that have invaded the body before they can spread. The second line of defense is not limited to specific pathogens but exerts a defensive effect against various pathogens and belongs to innate immunity. The third line of defense consists of immune organs (tonsils, lymph nodes, thymus, bone marrow, spleen, etc.) and immune cells (lymphocytes, phagocytes, etc.) delivered throughout the body via blood and lymphatic circulation; this corresponds to specific immunity, which acts only on specific pathogens or foreign substances. The third line of defense belongs to adaptive immunity. These three lines of defense act in a mutually complementary manner.

[0004] From a physiological perspective, the body's immunity naturally declines with age, particularly after the age of 40. When combined with irregular lifestyle and dietary habits, this can lead to a state of sub-health and an increased incidence of chronic diseases. From a social perspective, the prevalence of immune diseases is on the rise due to industrialization and environmental changes. Decreased immunity is a common clinical condition; symptoms include persistent fatigue, chronic colds with slow recovery, wounds that heal poorly or are susceptible to infection, and recurrent asthma, bronchitis, or diarrhea; in severe cases, it can lead to more serious illnesses.

[0005] In Korean traditional medicine (and Chinese medicine), the roots of the understanding of immunity can be traced back over 2,000 years. In Korean traditional medicine, this is referred to as *Jeonggi* (vital energy). The *Huangdi Neijing* (Yellow Emperor's Classic of Internal Medicine) records that "where Jeonggi is abundant, harmful energies cannot invade, and where harmful energies gather, Jeonggi is inevitably deficient." The strength or weakness of Jeonggi refers to the body's ability to prevent and combat disease. In a state of weak Jeonggi, external harmful energies can invade, or the physiological functions of internal organs weaken, leading to organ dysfunction, metabolic abnormalities of Qi, blood, and body fluids, and the accumulation of pathogenic products such as phlegm and dampness stasis, ultimately resulting in disease. The state of "weak Jeonggi" is similar to the etiology of immunodeficiency diseases in modern medicine, which involve abnormalities in the development, biochemical action, proliferation, regulation, or metabolism of immune cells. Research indicates that a deficiency and depletion of Jeonggi are major causes of various diseases. Therefore, "Jeonggi" is strengthened by nourishing Jeonggi, identifying the weakness of the constitution, and employing methods such as tonifying energy, nourishing blood, nourishing Yin, warming Yang, sequestering essence, and generating body fluids. This corrects physical imbalances and demonstrates excellent efficacy in strengthening immunity and improving the stability of the immune system. It leads to a healthy state where “vital energy is abundant, negative energy naturally disappears, and Yin and Yang are in harmony.”

[0006] Korean traditional medicine is characterized by a patient-centered approach to disease prevention and treatment, aiming for fundamental healing while alleviating symptoms tailored to individual needs. Complex herbal prescriptions enable symptom-specific diagnosis and treatment, provide complementary effects, and offer comprehensive efficacy with minimal side effects, making them applicable to the entire population with weakened immunity.

[0007]

[0008] One objective of the present invention is to provide a method for preparing a composition for boosting immunity.

[0009] Another objective of the present invention is to provide an immunity-enhancing composition prepared from medicinal materials including Angelica gigas, Cnidium officinale, and Paeonia lactiflora.

[0010] Another objective of the present invention is to provide a herbal medicine formulation comprising the above composition and a pharmaceutically acceptable excipient.

[0011] Another objective of the present invention is to provide a use for preparing a medicinal preparation intended for enhancing and / or regulating immunity of the above composition or the above herbal medicine preparation, or for treating and / or preventing deficiency of qi and blood.

[0012] Another objective of the present invention is to provide a use for preparing a drug intended to regulate bone marrow hematopoietic function or the PI3K-Akt signaling pathway of the above composition or the above herbal medicine preparation.

[0013] Another objective of the present invention is to provide an immune-enhancing use of the above composition or the above herbal medicine preparation.

[0014] Another objective of the present invention is to provide a method for enhancing immunity, comprising the step of administering the composition or the herbal medicine preparation to an individual.

[0015]

[0016] In order to solve the above problem,

[0017] An embodiment of the present invention embodying the present invention is a method for preparing a composition for enhancing immunity, comprising the step of taking medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora in a weight ratio of 1 to 10:1 to 10:1 to 10, preferably 10:4 to 8:10, more preferably 10:5:10;

[0018] 1 to 10 parts by weight of Angelica gigas are taken and decocted with water to extract, and the Angelica gigas hot water extract is concentrated. The hot water concentrate is divided into a first Angelica gigas hot water concentrate and a second Angelica gigas hot water concentrate so that the weight ratio of the first and second hot water concentrates is 1 to 10:10 to 1. 95 volume% of ethanol is added to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 50 to 90 volume%, after which an alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours. Then, the alcohol precipitate is concentrated again until the solid content reaches 30 weight% to obtain an Angelica gigas alcohol precipitate. The first Angelica gigas hot water concentrate and the Angelica gigas alcohol precipitate are mixed in a weight ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0 to obtain an Angelica gigas extract. The obtaining step;

[0019] 1 to 10 parts by weight of Cnidium officinale are taken and decocted with water to extract the extract, and the hot water extract of Cnidium officinale is concentrated. The hot water concentrate is divided into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1. Then, 95 volume% of ethanol is added to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 50 to 90 volume%, after which an alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours. Then, the alcohol precipitate is concentrated again until the solid content reaches 30 weight% to obtain an alcohol precipitate of Cnidium officinale. The first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0 to obtain a Cnidium officinale extract. The obtaining step;

[0020] A step of extracting 1 to 10 parts by weight of white peony by boiling in water, and concentrating the hot water extract of white peony to obtain a white peony extract;

[0021] The method comprises the step of obtaining the above-mentioned immune-enhancing composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 1 to 10:1 to 10:1 to 10, preferably 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, preferably in the specified weight ratio.

[0022] In one embodiment, the method comprises taking 1 to 10 parts by weight of Angelica gigas, first adding 10 times the amount of water (v / w) and decocting at 95°C for 4 hours, then adding 5 times the amount of water (v / w) and decocting at 95°C for 2 hours, combining both extracts and concentrating them to obtain a hot water concentrate of Angelica gigas, dividing the hot water concentrate of Angelica gigas into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas so that the weight ratio of the first hot water concentrate of Angelica gigas to the second hot water concentrate of Angelica gigas is 1 to 10:10 to 1, adding 95 volume% of ethanol to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 65 volume%, performing an alcohol precipitation treatment, taking the lower precipitate and leaving it at room temperature for at least 12 hours, and then repeating the process until the solid content of the alcohol precipitate reaches 30 weight%. A step of concentrating to obtain an Angelica alcohol precipitate, and mixing the first Angelica hot water concentrate and the Angelica alcohol precipitate in a weight ratio of 6.0:4.0 to obtain an Angelica extract;

[0023] Take 1 to 10 parts by weight of Cnidium officinale, first add 10 times the amount of water (v / w) and decoct at 95°C for 4 hours, then add 5 times the amount of water (v / w) and decoct at 95°C for 2 hours, combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale, divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1, add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 65% by volume, then perform an alcohol precipitation treatment, take the lower precipitate and leave it at room temperature for at least 12 hours, then concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain the Cnidium officinale alcohol precipitate A step of obtaining, and mixing the first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale in a weight ratio of 6:4 to obtain a Cnidium officinale extract;

[0024] A step of taking 1 to 10 parts by weight of white peony, first adding 10 v / w of water and boiling at 95°C for 4 hours, then adding 5 v / w of water and boiling at 95°C for 2 hours, combining both extracts and concentrating to a solid content of 30 wt% to obtain a white peony extract;

[0025] The method comprises the step of obtaining the above-mentioned immune-enhancing composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 2~8:2~5:2~9, 4~7:1~4:4~7, or 5~6:2~3:5~6.

[0026] Another aspect of the present invention embodying the present invention is a composition for boosting immunity prepared from medicinal materials including Angelica gigas, Cnidium officinale, and Paeonia lactiflora, wherein the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 1 to 10:1 to 10:1 to 10, preferably 10:4 to 8:10, and more preferably 10:5:10.

[0027] In one embodiment, the composition comprises an extract prepared from medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of the Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 1 to 10:1 to 10:1 to 10, preferably 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, more preferably 5.6:2.28:5.56, 2.8:2.28:5.56, 8.41:2.28:2.56, 5.6:2.28:2.78, 5.6:2.28:8.34, 5.6:4.56:2.78, or 2.8:4.56:5.56.

[0028] In one embodiment, the composition comprises taking 1 to 10 parts by weight of Angelica gigas, decocting it with water to extract it, concentrating the hot water extract of Angelica gigas, dividing the hot water concentrate into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas, such that the weight ratio of the first hot water concentrate of Angelica gigas and the second hot water concentrate of Angelica gigas is 1 to 10:10 to 1, adding 95 volume% of ethanol to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 50 to 90 volume%, and then performing an alcohol precipitation treatment. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours, and then concentrated again until the solid content of the alcohol precipitate reaches 30 weight% to obtain an Angelica gigas alcohol precipitate, and the first hot water concentrate of Angelica gigas and the Angelica gigas alcohol precipitate are in a ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0. A step of obtaining Angelica extract by mixing in a weight ratio;

[0029] 1 to 10 parts by weight of Cnidium officinale are taken and decocted with water to extract the extract, and the hot water extract of Cnidium officinale is concentrated. The hot water concentrate is divided into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1. Then, 95 volume% of ethanol is added to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 50 to 90 volume%, after which an alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours. Then, the alcohol precipitate is concentrated again until the solid content reaches 30 weight% to obtain an alcohol precipitate of Cnidium officinale. The first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0 to obtain a Cnidium officinale extract. The obtaining step;

[0030] A step of extracting 1 to 10 parts by weight of white peony by boiling in water, and concentrating the hot water extract of white peony to obtain a white peony extract;

[0031] The composition may be prepared by a method comprising the step of obtaining a composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 1 to 10:1 to 10:1 to 10, preferably 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, preferably in the specified weight ratio.

[0032] In one embodiment, the composition comprises taking 1 to 10 parts by weight of Angelica gigas, first adding 10 v / w times the amount of water and decocting at 95°C for 4 hours, then adding 5 v / w times the amount of water and decocting at 95°C for 2 hours, combining both extracts and concentrating them to obtain an Angelica gigas hot water concentrate with a solid content of 30 wt%, dividing the Angelica gigas hot water concentrate into an Angelica gigas first hot water concentrate and an Angelica gigas second hot water concentrate so that the weight ratio of the first and second hot water concentrates is 1 to 10:10 to 1, adding 95 volume% of ethanol to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 65 volume%, then performing an alcohol precipitation treatment, taking the lower precipitate and leaving it at room temperature for at least 12 hours, and then until the solid content of the alcohol precipitate reaches 30 wt%. A step of obtaining an Angelica alcohol precipitate by concentrating again, and obtaining an Angelica extract by mixing the first hot water concentrate of Angelica and the Angelica alcohol precipitate in a weight ratio of 6.0:4.0;

[0033] Take 1 to 10 parts by weight of Cnidium officinale, first add 10 times the amount of water (v / w) and decoct at 95°C for 4 hours, then add 5 times the amount of water (v / w) and decoct at 95°C for 2 hours, combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale, divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1, add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 65% by volume, then perform an alcohol precipitation treatment, take the lower precipitate and leave it at room temperature for at least 12 hours, then concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain the Cnidium officinale alcohol precipitate A step of obtaining, and mixing the first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract;

[0034] A step of taking 1 to 10 parts by weight of white peony, first adding 10 v / w of water and boiling at 95°C for 4 hours, then adding 5 v / w of water and boiling at 95°C for 2 hours, combining both extracts and concentrating to a solid content of 30 wt% to obtain a white peony extract;

[0035] The composition may be prepared by a method comprising the step of obtaining the composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 2~8:2~5:2~9, 4~7:1~4:4~7, or 5~6:2~3:5~6.

[0036] In one embodiment, the composition may be prepared with Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora may be 1 to 10:1 to 10:1 to 10, preferably 10:4 to 8:10, more preferably 10:5:10.

[0037] Another aspect of the present invention embodying the present invention is a herbal medicine preparation comprising the above composition and a pharmaceutically acceptable excipient, wherein the herbal medicine preparation is prepared in the form of an ointment, an oral liquid preparation, a decoction, a tea, granules, pills, powder, tablets, or capsules.

[0038] Another aspect of the present invention embodying the present invention is the use of the above composition or the above pharmaceutical preparation for preparing a medicine intended for enhancing and / or regulating immunity, or for treating and / or preventing deficiency of qi and blood.

[0039] Another aspect of the present invention embodying the present invention is the use of the above composition or the above pharmaceutical preparation for preparing a drug intended for regulating bone marrow hematopoietic function or regulating the PI3K-Akt signaling pathway.

[0040] Another aspect of the present invention embodying the present invention is the use of the above composition or the above pharmaceutical preparation for enhancing immunity.

[0041] Another aspect of the present invention embodying the present invention is a method for enhancing immunity, comprising the step of administering the composition or the pharmaceutical preparation to an individual.

[0042] The composition according to the present invention can be applied as an immune enhancer or a food composition for immune enhancement by improving immune function.

[0043] Figure 1 illustrates the experimental results of Example 9.

[0044] Figure 2 shows the experimental results of Example 11, where A is the distilled water group, B is the model group, and C is the high-capacity composition group.

[0045] Each description and embodiment disclosed herein may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the invention. Furthermore, the scope of the invention is not to be limited by the specific descriptions provided below.

[0046] In addition, a person skilled in the art can recognize or identify a number of equivalents to the specific embodiments of the invention described in this application using only ordinary experiments. In addition, such equivalents are intended to be included in the invention.

[0047] Furthermore, throughout the entire specification of this application, when a part is described as "including" a certain component, this means that, unless specifically stated otherwise, it does not exclude other components but may include additional components.

[0048]

[0049] The present invention will be described in more detail below.

[0050] One or more embodiments of the present invention provide an immune-boosting composition prepared with medicinal materials including Angelica gigas, Cnidium officinale, and Paeonia lactiflora, wherein the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is (1~10):(1~10):(1~10), for example, (1, 2, 3, 4, 5, 6, 7, 8, 9, 10):(1, 2, 3, 4, 5, 6, 7, 8, 9, 10):(1, 2, 3, 4, 5, 6, 7, 8, 9, 10).

[0051] In one or more embodiments, the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 10:(4-8):10, for example 10:5:10, 10:6:10, or 10:7:10.

[0052] In one or more embodiments, the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 10:5:10.

[0053] In one or more embodiments, the composition is composed of extracts prepared from medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of the Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 1 to 10:1 to 10:1 to 10, for example (1, 2, 3, 4, 5, 6, 7, 8, 9, 10):(1, 2, 3, 4, 5, 6, 7, 8, 9, 10):(1, 2, 3, 4, 5, 6, 7, 8, 9, 10).

[0054] In one or more embodiments, the composition is composed of extracts prepared from medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of the Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, for example, 5.6:2.28:5.56, 2.8:2.28:5.56, 8.41:2.28:2.56, 5.6:2.28:2.78, 5.6:2.28:8.34, 5.6:4.56:2.78, or 2.8:4.56:5.56.

[0055] In one or more embodiments, the composition is,

[0056] 1 to 10 parts by weight of Angelica gigas are taken and decocted with water to extract, then the Angelica gigas hot water extract is concentrated, and the hot water concentrate is divided into Angelica gigas first hot water concentrate and Angelica gigas second hot water concentrate, wherein the weight ratio of the Angelica gigas first hot water concentrate and the Angelica gigas second hot water concentrate is (1 to 10):(10 to 1) (e.g., 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, The ratios are adjusted to 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.5:1, 1:1), and 95 volume% ethanol is added to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 50–90 volume% (e.g., 55 volume%, 60 volume%, 65 volume%, 70 volume%, 75 volume%, 80 volume%, 85 volume%, 90 volume%), after which alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours (e.g., 12 hours, 15 hours, 20 hours, 24 hours), and then the solid content of the alcohol precipitate is The mixture is concentrated again until it reaches 30% by weight to obtain an Angelica alcohol precipitate, and the first hot water concentrate of Angelica and the Angelica alcohol precipitate are mixed in (1~10):(10~1)(e.g., 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.5:1, 1:1), or (6.0~7.0) : (3.0~4.0) (e.g.: 6.0:3.0, 6.0:3.5, 6.5:4.0, 6.5:3.0, 6.5:3.5, 6.5:4.0, 7.0:3.0, 7.A step of obtaining Angelica extract by mixing in a weight ratio of 0:3.5, 7.0:4.0;

[0057] 1 to 10 parts by weight of Cnidium officinale are taken and decocted with water to extract, then the hot water extract of Cnidium officinale is concentrated, and the hot water concentrate is divided into Cnidium officinale first hot water concentrate and Cnidium officinale second hot water concentrate, wherein the weight ratio of the Cnidium officinale first hot water concentrate and the Cnidium officinale second hot water concentrate is (1 to 10):(10 to 1) (e.g., 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, The ratios are adjusted to 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.5:1, 1:1), and 95 vol% ethanol is added to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 50–90 vol% (e.g., 55 vol%, 60 vol%, 65 vol%, 70 vol%, 75 vol%, 80 vol%, 85 vol%, 90 vol%), after which alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours (e.g., 12 hours, 15 hours, 20 hours, 24 hours), and then the solid content of the alcohol precipitate is The mixture is concentrated again until it reaches 30% by weight to obtain an alcohol precipitate of Angelica gigas, and the first hot water concentrate of Angelica gigas and the alcohol precipitate of Angelica gigas are mixed in a ratio of (1~10):(10~1)(e.g., 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.5:1, 1:1), or 6.0~7.0:3.0~4.0 (e.g., 6.0:3.0, 6.0:3.5, 6.5:4.0, 6.5:3.0, 6.5:3.5, 6.5:4.0, 7.0:3.0, 7.0:3.0.A step of obtaining Cnidium officinale extract by mixing in a weight ratio of 5, 7.0:4.0;

[0058] A step of extracting 1 to 10 parts by weight of white peony by boiling in water, and concentrating the hot water extract of white peony to obtain a white peony extract;

[0059] A method comprising the step of obtaining a composition by mixing the above Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of (1-10):(1-10):(1-10) is prepared.

[0060] In one or more embodiments, the composition is composed of extracts prepared from medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of the Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 1 to 10 (e.g., weight ratio 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10): 1 to 10 (e.g., weight ratio 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10): 1 to 10 (e.g., weight ratio 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10), and may be, for example, 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6.

[0061] In one or more embodiments, the composition is obtained by mixing Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 5.6:2.28:5.56.

[0062] In one or more embodiments, the composition comprises taking 1 to 10 parts by weight of Angelica gigas, first adding 10 v / w times the amount of water and decocting at 95°C for 4 hours, then adding 5 v / w times the amount of water and decocting at 95°C for 2 hours, combining both extracts and concentrating them so that the solid content is 30 wt% to obtain an Angelica gigas hot water concentrate, dividing the Angelica gigas hot water concentrate into an Angelica gigas first hot water concentrate and an Angelica gigas second hot water concentrate so that the weight ratio of the Angelica gigas first hot water concentrate to the Angelica gigas second hot water concentrate is (1 to 10): (10 to 1), adding 95 volume% of ethanol to the Angelica gigas second hot water concentrate to adjust the ethanol concentration of the extract to 65 volume%, then performing an alcohol precipitation treatment, taking the lower precipitate and leaving it at room temperature for at least 12 hours, and then the solid content of the alcohol precipitate is 30 wt% A step of obtaining an Angelica alcohol precipitate by concentrating again until sufficient, and obtaining an Angelica extract by mixing the first hot water concentrate of Angelica and the Angelica alcohol precipitate in a weight ratio of 6.0:4.0;

[0063] Take 1 to 10 parts by weight of Cnidium officinale, first add 10 times the amount of water (v / w) and decoct at 95°C for 4 hours, then add 5 times the amount of water (v / w) and decoct at 95°C for 2 hours, combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale, divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first and second hot water concentrates is (1–10):(10–1), add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 65% by volume, then perform an alcohol precipitation treatment, take the lower precipitate and leave it at room temperature for at least 12 hours, then concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain Cnidium officinale alcohol A step of obtaining a precipitate, and mixing the first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract;

[0064] A step of taking 1 to 10 parts by weight of white peony, first adding 10 v / w of water and boiling at 95°C for 4 hours, then adding 5 v / w of water and boiling at 95°C for 2 hours, combining both extracts and concentrating to a solid content of 30 wt% to obtain a white peony extract;

[0065] The composition is prepared by a method comprising the step of obtaining the composition by mixing the above Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 5.6:2.28:5.56.

[0066] In one or more embodiments, the composition is composed of extracts prepared from medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 2~8:2~5:2~9, 4~7:1~4:4~7, or 5~6:2~3:5~6.

[0067] In one or more embodiments, the composition is prepared from Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is (1 to 10) (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10):(1 to 10) (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10):(1 to 10) (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10).

[0068] In one or more embodiments, the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 10:(4~8):10.

[0069] In one or more embodiments, the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 10:5:10.

[0070] One or more embodiments of the present invention provide a herbal medicine formulation comprising the composition of the present invention and a pharmaceutically acceptable excipient.

[0071] In one or more embodiments, the herbal medicine preparation is prepared in the form of an ointment, an oral liquid preparation, a decoction, a tea, granules, pills, powder, tablets, or capsules.

[0072] One or more embodiments of the present invention provide a use for preparing a medicine for the purpose of enhancing and / or regulating immunity, of the composition of the present invention or the herbal medicine preparation of the present invention.

[0073] One or more embodiments of the present invention provide a use for the composition of the present invention or the herbal medicine preparation of the present invention for preparing a medicine intended for the treatment and / or prevention of qi and blood deficiency.

[0074] One or more embodiments of the present invention provide a use for preparing a drug intended for regulating bone marrow hematopoietic function or regulating the PI3K-Akt signaling pathway using the composition of the present invention or the herbal medicine preparation of the present invention.

[0075] One or more embodiments of the present invention provide a method for preparing a composition, wherein the method comprises:

[0076] A step of taking medicinal materials such as Angelica gigas, Cnidium officinale, and Paeonia lactiflora in proportions such as 4.0–8.0 parts of Angelica gigas, 1.0–3.0 parts of Cnidium officinale, and 4.0–8.0 parts of Paeonia lactiflora; 0.1–1.0 parts of Angelica gigas, 0.1–1.0 parts of Cnidium officinale, and 0.1–1.0 parts of Paeonia lactiflora; 1.0 parts of Angelica gigas, 0.4–0.8 parts of Cnidium officinale, and 1.0 parts of Paeonia lactiflora; or 1.0 parts of Angelica gigas, 0.5 parts of Cnidium officinale, and 1.0 parts of Paeonia lactiflora;

[0077] 1 to 10 parts by weight of Angelica gigas are taken and decocted with water to extract, then the Angelica gigas hot water extract is concentrated, and the hot water concentrate is divided into Angelica gigas first hot water concentrate and Angelica gigas second hot water concentrate, wherein the weight ratio of the Angelica gigas first hot water concentrate and the Angelica gigas second hot water concentrate is (1 to 10):(10 to 1) (e.g., 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, The ratios are adjusted to 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.5:1, 1:1), and 95 volume% ethanol is added to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 50–90 volume% (e.g., 55 volume%, 60 volume%, 65 volume%, 70 volume%, 75 volume%, 80 volume%, 85 volume%, 90 volume%), after which alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours (e.g., 12 hours, 15 hours, 20 hours, 24 hours), and then the solid content of the alcohol precipitate is A step of obtaining an Angelica alcohol precipitate by concentrating again until it reaches 30% by weight, and obtaining an Angelica extract by mixing the first hot water concentrate of Angelica and the Angelica alcohol precipitate in a weight ratio of (6.0~7.0) : (3.0~4.0) (e.g., 6.0:3.0, 6.0:3.5, 6.5:4.0, 6.5:3.0, 6.5:3.5, 6.5:4.0, 7.0:3.0, 7.0:3.5, 7.0:4.0);

[0078] 1 to 10 parts by weight of Cnidium officinale are taken and decocted with water to extract, then the hot water extract of Cnidium officinale is concentrated, and the hot water concentrate is divided into Cnidium officinale first hot water concentrate and Cnidium officinale second hot water concentrate, wherein the weight ratio of the Cnidium officinale first hot water concentrate and the Cnidium officinale second hot water concentrate is (1 to 10):(10 to 1) (e.g., 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10, 10:1, 9.5:1, 9:1, 8.5:1, 8:1, 7.5:1, The ratios are adjusted to 7:1, 6.5:1, 6:1, 5.5:1, 5:1, 4.5:1, 4:1, 3.5:1, 3:1, 2.5:1, 2:1, 1.5:1, 1:1), and 95 vol% ethanol is added to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 50–90 vol% (e.g., 55 vol%, 60 vol%, 65 vol%, 70 vol%, 75 vol%, 80 vol%, 85 vol%, 90 vol%), after which alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours (e.g., 12 hours, 15 hours, 20 hours, 24 hours), and then the solid content of the alcohol precipitate is A step of obtaining an alcohol precipitate of Angelica gigas by concentrating again until it reaches 30% by weight, and obtaining an Angelica gigas extract by mixing the first hot water concentrate of Angelica gigas and the Angelica gigas alcohol precipitate in a weight ratio of (6.0~7.0) : (3.0~4.0) (e.g., 6.0:3.0, 6.0:3.5, 6.5:4.0, 6.5:3.0, 6.5:3.5, 6.5:4.0, 7.0:3.0, 7.0:3.5, 7.0:4.0);

[0079] A step of extracting 1 to 10 parts by weight of white peony by boiling in water, and concentrating the hot water extract of white peony to obtain a white peony extract;

[0080] The method includes the step of obtaining a composition by mixing the above Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of (1-10):(1-10):(1-10).

[0081] In one or more embodiments, the weight ratio of the Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6.

[0082] The preparation method provided by one or more embodiments of the present invention comprises taking 1 to 10 parts by weight of Angelica gigas, first adding 10 times the amount of water (v / w) and decocting at 95°C for 4 hours, then adding 5 times the amount of water (v / w) and decocting at 95°C for 2 hours, combining both extracts and concentrating them so that the solid content is 30% by weight to obtain an Angelica gigas hot water concentrate, dividing the Angelica gigas hot water concentrate into an Angelica gigas first hot water concentrate and an Angelica gigas second hot water concentrate so that the weight ratio of the Angelica gigas first hot water concentrate to the Angelica gigas second hot water concentrate is (1 to 10): (10 to 1), adding 95% by volume of ethanol to the Angelica gigas second hot water concentrate to adjust the ethanol concentration of the extract to 65% by volume, performing an alcohol precipitation treatment, taking the lower precipitate and leaving it at room temperature for at least 12 hours, and then the solid content of the alcohol precipitate A step of obtaining an Angelica alcohol precipitate by concentrating again until it reaches 30% by weight, and obtaining an Angelica extract by mixing the first hot water concentrate of Angelica and the Angelica alcohol precipitate in a weight ratio of 6.0:4.0;

[0083] Take 1 to 10 parts by weight of Cnidium officinale, first add 10 times the amount of water (v / w) and decoct at 95°C for 4 hours, then add 5 times the amount of water (v / w) and decoct at 95°C for 2 hours, combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale, divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first and second hot water concentrates is (1–10):(10–1), add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 65% by volume, then perform an alcohol precipitation treatment, take the lower precipitate and leave it at room temperature for at least 12 hours, then concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain Cnidium officinale alcohol A step of obtaining a precipitate, and mixing the first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract;

[0084] A step of taking 1 to 10 parts by weight of white peony, first adding 10 v / w of water and boiling at 95°C for 4 hours, then adding 5 v / w of water and boiling at 95°C for 2 hours, combining both extracts and concentrating to a solid content of 30 wt% to obtain a white peony extract;

[0085] A composition is obtained by mixing Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in weight ratios of 2~8:2~5:2~9, 4~7:1~4:4~7, and 5~6:2~3:5~6, respectively.

[0086] The present invention relates to the prevention and treatment of immune deficiency. By combining the traditional Korean medicine theory that "when vital energy is abundant, bad energy naturally disappears" with modern pharmacological research, the invention enhances immunity and, specifically, provides a composition and a method for preparing the same applicable to the immune deficiency caused by the syndrome of Qi and Blood Yang deficiency in Korean medicine. The present invention is effective in treating not only the symptoms but also the underlying causes.

[0087] In one or more embodiments of the present invention, the composition of the present invention may be used as a pharmaceutical product for the prevention and treatment of diseases related to immune deficiency.

[0088] In one or more embodiments of the present invention, the composition of the present invention is characterized by having an excellent immune-boosting effect, being easy to prepare, and having high safety.

[0089] In one or more embodiments of the present invention, the method for preparing the composition of the present invention is highly efficient and has a high utilization rate of the active ingredient, which can also contribute to the utilization of medicinal resources.

[0090] In the present invention, the dried root of Angelica sinensis (Oliv.) Diels, a plant of the Apiaceae family, may be used as the Angelica root. This is distinct from the roots of Angelica gigas Nakai (Korean Angelica) of the Apiaceae family, Angelica acutiloba (Siebold. & Zucc.) Kitag. (Japanese Angelica) or Angelica acutiloba (Siebold. & Zucc.) Kitag. var. sugiyamae Hikino (Japanese Angelica) of the Apiaceae family in Japan. Angelica sinensis has a warm energy and a sweet yet pungent taste; generally, Angelica sinensis has a stronger sweet taste and a weaker spiciness compared to Angelica gigas Nakai. The efficacy of Angelica sinensis is a blood-replenishing effect that generates blood when there is a blood deficiency, and Angelica made from the root of Angelica sinensis has an excellent blood-replenishing effect. However, Angelica root, made from the roots of the Korean angelica, is more effective in promoting smooth blood circulation than in tonifying blood, and it has strong anticancer and blood pressure-lowering effects. Pharmacologically, Angelica is known to promote coronary artery blood flow and stimulate red blood cell production.

[0091] In this invention, the dried rhizome of *Ligusticum chuanxiong Hort.* or *Ligusticum wallichii Franch.*, a plant of the Apiaceae family, may be used as *Cheongung*. *Cheongung* is a perennial herb belonging to the Apiaceae family, order Apiaceae, class Dicotyledonous plants, and is generally cultivated as a medicinal plant. It is effective for sedation, pain relief, and tonic purposes, and is used to treat headaches, anemia, and gynecological diseases. The rhizome is dug up between September and November, the leaves and stems are removed, and after drying in the sun, it is decocted for consumption or used in the form of pills or powder.

[0092] Paeonia lactiflora Pall., a plant of the Ranunculaceae family, is a perennial herb belonging to the genus Paeonia in the family Paeoniaceae that grows in mountainous regions. It is used for horticulture due to its beautiful flowers. Additionally, its roots are used as a medicinal ingredient for pain relief, abdominal pain, menstrual cramps, amenorrhea, hemoptysis, anemia, and bruises. In China, it was already cultivated as an ornamental plant during the Qin and Ming dynasties, giving it a longer history of cultivation than the tree peony. Through the Song and Qing dynasties, dozens of varieties were recorded, and it is distributed in Korea, Mongolia, and Eastern Siberia.

[0093] In the present invention, the white peony root may be used in its dried form.

[0094] In one or more embodiments, Angelica sinensis serves as the principal herb, possessing the efficacy of nourishing blood and harmonizing yin; it replenishes deficiency syndromes and treats the underlying causes. Its acidic nature prevents blood stasis. Paeonia lactiflora serves as the auxiliary herb, possessing the efficacy of nourishing blood and relieving yin; it assists the blood-nourishing action of Angelica sinensis and harmonizes the stomach and intestines by strengthening the dispersing function through its liver-softening action. Ligusticum chuanxiong is a herb that promotes the circulation of qi within the blood; it facilitates blood circulation and has the efficacy of dissolving blood stasis, ensuring that qi and blood flow freely without obstruction. Ligusticum chuanxiong serves as a regulating herb, playing a role in harmonizing the actions of the drugs.

[0095] In the present invention, the term "extract" may be an extract containing the Angelica gigas, Cnidium officinale, or Paeonia lactiflora; or may be a mixture of an extract obtained by extracting the Angelica gigas, Cnidium officinale, or Paeonia lactiflora and a polysaccharide isolate obtained from the obtained extract.

[0096] In one embodiment of the present invention, the extract comprises the extract itself and all formulations of extracts that can be formed using the extract, such as an extract obtained by extraction treatment of Angelica gigas, Cnidium officinale, or Paeonia lactiflora, a polysaccharide isolate of the extract, a diluted or concentrated extract, a dried product obtained by drying the extract, a modified or purified product thereof, or a mixture thereof.

[0097] The above extract may be extracted with a solvent selected from the group consisting of water, organic solvents, and mixed solvents thereof, but is not limited thereto.

[0098] If the solvent of the above extract is water, it may include a cold water extract or a hot water extract.

[0099] In one embodiment, the organic solvent may be a straight-chain or branched alcohol having 1 to 6 carbon atoms, specifically one or more selected from the group consisting of methanol, ethanol, propanol, butanol, pentanol, and hexanol, and more specifically ethanol, but is not limited thereto.

[0100] In one embodiment, the organic solvent may be a hydrocarbon solvent such as glycerol, ethylene glycol, propylene glycol, methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, chloroform, or a non-polar organic solvent such as petroleum ether, methyl acetate, benzene, hexane, chloroform, methylene chloride, dimethyl ether, ethyl acetate, but is not limited thereto.

[0101] The above solvent may also include an aqueous solution of an organic solvent, and its concentration is not particularly limited but may be 1 to 99% (v / v), specifically 60 to 98% (v / v), more specifically 80 to 95% (v / v), and even more specifically 95% (v / v).

[0102] In one embodiment of the present invention, the extraction temperature may be in the range of 20°C to 100°C, and the extraction period may be an extract obtained using an extraction method such as hot water extraction, cold maceration extraction, reflux cooling extraction, or ultrasonic extraction for about 1 hour to 10 days, but is not limited to the extraction temperature, extraction period, or extraction method.

[0103] In the present invention, the term "polysaccharide isolate" comprises a polysaccharide precipitated by adding a solvent to the extracts of Angelica gigas, Cnidium officinale, or Paeonia lactiflora, wherein the polysaccharide is also referred to as a polysaccharide and may comprise one or more polysaccharides.

[0104] The method for obtaining the polysaccharide isolate in the present invention is not particularly limited and may be carried out according to methods commonly used in the art. Non-limiting examples of the above method include a polysaccharide isolate obtained from an extract of Angelica gigas, Cnidium officinale, or Paeonia lactiflora of the present invention by treating the extract with a predetermined solvent, and, as an example, a polysaccharide isolate produced by adding ethanol to the extract of Angelica gigas, Cnidium officinale, or Paeonia lactiflora of the present invention, but is not limited thereto.

[0105] In one embodiment of the present invention, a polysaccharide isolate produced by adding alcohol to an extract of Angelica gigas, Cnidium officinale, or Paeonia lactiflora may be mixed with an “alcohol precipitate.”

[0106] In the present invention, the type of solvent used to obtain the polysaccharide isolate is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the solvent may include water, organic solvents, or a mixture thereof, and these may be used alone or in combination of one or more, but are not limited thereto.

[0107] In one embodiment, the organic solvent may be a straight-chain or branched alcohol having 1 to 6 carbon atoms, specifically one or more selected from the group consisting of methanol, ethanol, propanol, butanol, pentanol, and hexanol, specifically ethanol, but is not limited thereto.

[0108] In addition, the organic solvent may be one or more selected from the group consisting of dichloromethane, diethyl ether, chloroform, and ethyl acetate, but is not limited thereto.

[0109] In one embodiment of the present invention, the polysaccharide isolate may be an ethanol polysaccharide isolate, but is not limited thereto.

[0110] In one embodiment, the ethanol polysaccharide isolate may be a 60% to 100% ethanol polysaccharide isolate, a 70% to 100% ethanol polysaccharide isolate, an 80% to 100% ethanol polysaccharide isolate, an 85% to 100% ethanol polysaccharide isolate, a 90% to 100% ethanol polysaccharide isolate, a 95% to 100% ethanol polysaccharide isolate, an 80% to 95% ethanol polysaccharide isolate, or an 85% to 95% ethanol polysaccharide isolate; specifically, it may be a 60%, 70%, 80%, 85%, 90%, 95%, or 100% ethanol polysaccharide isolate, but is not limited thereto.

[0111] In the present invention, the term "mixture" refers to a mixture obtained by mixing an extract of Angelica gigas, Cnidium officinale, or Paeonia lactiflora prepared in the present invention with a polysaccharide isolate obtained by treating each of the said extracts with a predetermined solvent, and the mixing ratio is not particularly limited.

[0112] By using the extract of each of the above-mentioned plants in combination with their polysaccharide isolates, the above-mentioned composition can achieve significantly superior effects compared to cases containing only the extract, polysaccharide isolate, or polysaccharide of a single plant. Furthermore, the above-mentioned composition can exhibit significantly superior immune-enhancing or strengthening effects compared to cases containing plant extracts, polysaccharide isolates, or polysaccharides of plants other than Angelica gigas, Cnidium officinale, and Paeonia lactiflora.

[0113] When the above mixture is a mixture of an extract and a polysaccharide isolate, the extract and the polysaccharide isolate may be included in a weight ratio of about 40 to 80:20 to 60, for example, in a weight ratio of about 45 to 75:25 to 55, 50 to 70:30 to 50, or 60 to 70:30 to 40, and specifically in a weight ratio of 60:40, but is not limited thereto.

[0114] In one or more embodiments, 95 volume% of ethanol is added to a portion of the hot water extract concentrate of Angelica gigas and Cnidium officinale.

[0115] In one or more embodiments, the composition of the present invention has an excellent taste.

[0116] In one or more embodiments, the composition of the present invention has excellent transparency.

[0117] One or more embodiments of the present invention provide a composition having an immune-boosting effect, and the composition includes Angelica gigas, Cnidium officinale, and Paeonia lactiflora.

[0118] In one or more embodiments, the above components comprise 10 to 1,000 parts of Angelica gigas, 5 to 1,000 parts of Cnidium officinale, and 10 to 1,000 parts of Paeonia lactiflora based on weight percentage.

[0119] In one or more embodiments, the above components comprise 6.0 parts of Angelica gigas, 2.0 parts of Cnidium officinale, and 6.0 parts of Paeonia lactiflora based on weight percentage.

[0120] In one or more embodiments, the method for preparing the composition comprises the following steps.

[0121] S1. Take Angelica gigas, first add 10 times the amount of water, and decoct at 95°C for 4 hours, then add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate to 30 Brix to obtain A. Divide A into A1 and A2. A2 is subjected to alcohol precipitation treatment using 95 vol% ethanol, adjusted to an ethanol concentration of 65 vol%, and left at room temperature for at least 12 hours. Concentrate treatment is performed to obtain A3, with a solid content of 30 wt%. A1 and A3 are mixed in a ratio of 6:4 to obtain Angelica gigas extract.

[0122] S2. Take Cnidium officinale, first add 10 times the amount of water and boil at 95°C for 4 hours, then add 5 times the amount of water and boil at 95°C for 2 hours. Combine both extracts and concentrate to 30 Brix to obtain B. Divide B into B1 and B2. B2 is subjected to alcohol precipitation treatment by adjusting the ethanol concentration to 65% by volume with 95% by volume ethanol, and left at room temperature for at least 12 hours. Concentrate treatment is performed to obtain B3 with a solid content of 30% by weight. B1 and B3 are mixed in a ratio of 6:4 to obtain Cnidium officinale extract.

[0123] S3. Take white peony root, first add 10 times the amount of water and boil at 95°C for 4 hours, then add 5 times the amount of water and boil at 95°C for 2 hours. Combine both extracts and concentrate to 30 Brix to obtain white peony extract.

[0124] S4. 5.60 parts of Angelica gigas extract, 2.28 parts of Cnidium officinale extract, and 5.56 parts of Paeonia lactiflora extract are taken to obtain a composition extract.

[0125] In one or more embodiments, the composition extract obtained by the above preparation method is mixed with an excipient to form an ointment, oral liquid preparation, beverage, decoction, tea, granule, pill, powder, tablet, or capsule.

[0126] In one or more embodiments, by adopting specific processes and dosage ratios, the composition exhibits a more excellent immunomodulatory effect and is suitable for applications related to regulating bodily immunity.

[0127] In one or more embodiments, the immune modulatory effect can be obtained by enhancing the body's immune function through the regulation of bone marrow hematopoietic function or the regulation of the PI3K-Akt signaling pathway.

[0128] In one or more embodiments, the composition may be used in pharmaceuticals and health foods and is suitable for a population with reduced immunity.

[0129] In one or more embodiments, the composition may be added to a food composition, and the food composition may be a health functional food composition.

[0130] The above food composition may include a food-grade acceptable carrier.

[0131] The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food, and food additives, and said types of food compositions can be prepared in various forms according to conventional methods known in the art.

[0132] When the composition of the present invention is used as a food additive, the composition may be added as is or used together with other foods or food ingredients, and may be used appropriately according to conventional methods. The amount of the active ingredient can be appropriately determined according to the purpose of use (prevention, health, or therapeutic treatment). Generally, when manufacturing food or beverages, the composition of the present invention is added in an amount of 0.0001 to 90 weight%, preferably 0.001 to 50 weight%, of the raw material composition. However, in the case of long-term consumption for the purpose of health and hygiene or health control, the amount may be used in an amount less than the above range.

[0133] There are no special restrictions on the types of food mentioned above. Examples of foods to which the above composition may be added include meat, sausage, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, and may include all health foods in the conventional sense.

[0134] The health beverage composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients, as in conventional beverages. The natural carbohydrates mentioned above are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As sweeteners, natural sweeteners such as taumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrates may generally be about 0.001 to 50 parts by weight, specifically about 0.01 to 30 parts by weight, per 100 parts by weight of the composition of the present invention.

[0135] In addition to the above, the composition of the present invention may contain various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. Although the proportion of these additives is not critical, they are generally selected in the range of 0.01 to 30 parts by weight per 100 parts by weight of the composition of the present invention. Furthermore, the composition of the present invention may contain fruit pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages. Although the proportion of such fruit pulp is not critical, it is generally selected in the range of 0.01 to 30 parts by weight per 100 parts by weight of the composition of the present invention, and these components may be used independently or in combination.

[0136] Another aspect of the present invention for achieving the above objective provides a health supplement comprising the composition of the present invention as an active ingredient.

[0137] The above term “health supplement” may include all known ranges of substances that play an auxiliary role for health.

[0138] The composition of this product is suitable for individuals with weakened immunity, particularly those exhibiting symptoms of Qi and Blood deficiency. These symptoms include fatigue, general weakness, exhaustion, a weak voice, lethargy, listlessness, lower back pain, heaviness in the legs, increased fatigue after exercise, frequent sweating, a pale complexion, and dry skin. Additionally, patients dislike cold and wind, catch colds easily, and have reduced recovery abilities. In women, menstrual flow may be light or pale in color, or amenorrhea may occur. Other accompanying symptoms may include loss of appetite, a pale tongue, a thin white coating, and a weak pulse. Traditional Korean medicine views the fundamental causes of weakened immunity as stemming from deficiency of essence and imbalances in Qi and Blood. It has long been said, "If Qi and Blood are vigorous, no disease can afflict you." The *Huangdi Neijing* states, "What a person possesses is only blood and qi," and the *Jingyue Quanshu* states, "Humans possess Yin and Yang, which are akin to blood and qi. Since Yang governs qi, the spirit is vigorous when qi is abundant; since Yin governs blood, the physical form is strong when it is abundant. Life relies solely on these." The *Yilin Kaichao* states, "The core of treating diseases lies in understanding qi and blood." Therefore, the key to preventing and treating a decline in immunity lies in tonifying qi and yang blood, and circulating qi and invigorating blood. Based on the Korean medical causes and pathophysiology of a decline in immunity, this invention improves symptoms by nourishing qi and blood and resolves the root cause, thereby bringing about the effect of strengthening vital energy and solidifying the foundation.

[0139] The composition described herein acts effectively on diseases with deficiency of both Qi and blood as the primary etiology, simultaneously nourishes Qi and blood, circulates Qi to resolve stagnation, and eliminates blood stasis through nourishing and invigorating blood. There are no contraindications for concomitant use, and since the efficacy of each component is mutually complementary, it is highly effective for boosting immunity.

[0140] One or more embodiments of the present invention provide the use of the above composition or the above herbal medicine preparation for boosting immunity.

[0141] One or more embodiments of the present invention provide a method for enhancing immunity, comprising the step of administering the above composition or the above herbal medicine preparation to an individual.

[0142] In the present invention, the term "administration" refers to introducing a composition of the present invention to a subject by any appropriate method, and the route of administration may be administered via various oral or parenteral routes as long as it can reach the target tissue.

[0143] In the present invention, the individual may include humans or non-human species, and more specifically, may include mammals including humans, and even more specifically, may include humans, dogs, rats, rabbits, cats, horses, or cattle, but may include any individual whose immunity can be enhanced by the administration of the composition of the present invention without limitation.

[0144]

[0145] To clarify the purpose, technical solution, and advantages of the present invention, the technical solution of the present invention is described in detail through the following specific embodiments; however, the present invention is not limited to the embodiments below. Any other embodiments that a person skilled in the art can obtain without creative effort based on the embodiments of the present invention are deemed to be included within the scope of the claims of the present invention.

[0146]

[0147] Sample preparation

[0148] Example 1: Preparation of Composition C1 of the present invention

[0149] Ten parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then five times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain an Angelica gigas hot water concentrate. The Angelica gigas hot water concentrate is divided into an Angelica gigas hot water concentrate No. 1 and an Angelica gigas hot water concentrate No. 2. 95% by volume of ethanol is added to the Angelica gigas hot water concentrate No. 2 to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an Angelica gigas alcohol precipitate. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0150] Take 5 parts by weight of Cnidium officinale, first add water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) and decoct at 95°C for 4 hours, then add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale. Divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. Add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After leaving at room temperature for at least 12 hours, concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0151] 10 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a hot water concentrate of white peony root.

[0152] Composition C1 is obtained by taking 5.60 parts of Angelica gigas extract, 2.28 parts of Cnidium officinale extract, and 5.56 parts of Paeonia lactiflora extract.

[0153]

[0154] Example 2: Preparation of Composition C2 of the present invention

[0155] 5 parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain a hot water concentrate of Angelica gigas. The hot water concentrate of Angelica gigas is divided into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas. 95% by volume of ethanol is added to the second hot water concentrate of Angelica gigas to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an alcohol precipitate of Angelica gigas. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0156] Take 5 parts by weight of Cnidium officinale, first add water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) and decoct at 95°C for 4 hours, then add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale. Divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. Add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After leaving at room temperature for at least 12 hours, concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0157] 10 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a hot water concentrate of white peony root.

[0158] Composition C2 is obtained by taking 2.80 parts of Angelica gigas extract, 2.28 parts of Cnidium officinale extract, and 5.56 parts of Paeonia lactiflora extract.

[0159]

[0160] Example 3: Preparation of Composition C3 of the present invention

[0161] 15 parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal weight (g)) is added and boiled at 95°C for 4 hours, then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain a hot water concentrate of Angelica gigas. The hot water concentrate of Angelica gigas is divided into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas. 95% by volume of ethanol is added to the second hot water concentrate of Angelica gigas to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an alcohol precipitate of Angelica gigas. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0162] Take 5 parts by weight of Cnidium officinale, first add water equivalent to 10 v / w times (ratio of water volume (L) to medicinal weight (g)) and decoct at 95°C for 4 hours, then add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale. Divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. Add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After leaving at room temperature for at least 12 hours, concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0163] 10 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a hot water concentrate of white peony root.

[0164] Composition C3 is obtained by taking 8.41 parts of Angelica gigas extract, 2.28 parts of Cnidium officinale extract, and 2.56 parts of Paeonia lactiflora extract.

[0165]

[0166] Example 4: Preparation of Composition C4 of the present invention

[0167] Ten parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then five times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain an Angelica gigas hot water concentrate. The Angelica gigas hot water concentrate is divided into an Angelica gigas hot water concentrate No. 1 and an Angelica gigas hot water concentrate No. 2. 95% by volume of ethanol is added to the Angelica gigas hot water concentrate No. 2 to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an Angelica gigas alcohol precipitate. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0168] Take 5 parts by weight of Cnidium officinale, first add water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) and decoct at 95°C for 4 hours, then add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale. Divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. Add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After leaving at room temperature for at least 12 hours, concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0169] 5 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a hot water concentrate of white peony root.

[0170] Composition C4 is obtained by taking 5.60 parts of Angelica gigas extract, 2.28 parts of Cnidium officinale extract, and 2.78 parts of Paeonia lactiflora extract.

[0171]

[0172] Example 5: Preparation of Composition C5 of the Present Invention

[0173] Ten parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then five times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain an Angelica gigas hot water concentrate. The Angelica gigas hot water concentrate is divided into an Angelica gigas hot water concentrate No. 1 and an Angelica gigas hot water concentrate No. 2. 95% by volume of ethanol is added to the Angelica gigas hot water concentrate No. 2 to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an Angelica gigas alcohol precipitate. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0174] Take 5 parts by weight of Cnidium officinale, first add water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) and decoct at 95°C for 4 hours, then add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale. Divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. Add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After leaving at room temperature for at least 12 hours, concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0175] 15 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a hot water concentrate of white peony root.

[0176] Composition C5 is obtained by taking 5.60 parts of Angelica gigas extract, 2.28 parts of Cnidium officinale extract, and 8.34 parts of Paeonia lactiflora extract.

[0177]

[0178] Example 6: Preparation of Composition C6 of the present invention

[0179] Ten parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then five times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain an Angelica gigas hot water concentrate. The Angelica gigas hot water concentrate is divided into an Angelica gigas hot water concentrate No. 1 and an Angelica gigas hot water concentrate No. 2. 95% by volume of ethanol is added to the Angelica gigas hot water concentrate No. 2 to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an Angelica gigas alcohol precipitate. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0180] Ten parts by weight of Cnidium officinale are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and decocted at 95°C for 4 hours, and then five times the amount of water is added and decocted at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain a hot water concentrate of Cnidium officinale. The hot water concentrate of Cnidium officinale is divided into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. 95% by volume of ethanol is added to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0181] 5 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a hot water concentrate of white peony root.

[0182] Composition C6 is obtained by taking 5.60 parts of Angelica gigas extract, 4.56 parts of Cnidium officinale extract, and 2.78 parts of Paeonia lactiflora extract.

[0183]

[0184] Example 7: Preparation of Composition C7 of the present invention

[0185] 5 parts by weight of Angelica gigas are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain a hot water concentrate of Angelica gigas. The hot water concentrate of Angelica gigas is divided into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas. 95% by volume of ethanol is added to the second hot water concentrate of Angelica gigas to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an alcohol precipitate of Angelica gigas. Angelica extract is obtained by mixing the first hot water concentrate of Angelica and the alcohol precipitate of Angelica in a weight ratio of 6.0:4.0.

[0186] Ten parts by weight of Cnidium officinale are taken, and water corresponding to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and decocted at 95°C for 4 hours, and then five times the amount of water is added and decocted at 95°C for 2 hours. The extracts from both are combined and concentrated until the solid content is 30% by weight to obtain a hot water concentrate of Cnidium officinale. The hot water concentrate of Cnidium officinale is divided into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale. 95% by volume of ethanol is added to the second hot water concentrate of Cnidium officinale to perform alcohol precipitation treatment at a concentration of 65% by volume. After being left at room temperature for at least 12 hours, the alcohol precipitate is concentrated again until the solid content is 30% by weight to obtain an alcohol precipitate of Cnidium officinale. A first hot water concentrate of Cnidium officinale and an alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract.

[0187] 5 parts by weight of white peony root are taken, and water equivalent to 10 v / w times (ratio of water volume (L) to medicinal material weight (g)) is added and boiled at 95°C for 4 hours, and then 5 times the amount of water is added and boiled at 95°C for 2 hours. The extracts from both are combined and concentrated to a solid content of 30% by weight to obtain a white peony root hot water concentrate.

[0188] Composition C7 is obtained by taking 2.80 parts of Angelica gigas extract, 4.56 parts of Cnidium officinale extract, and 5.56 parts of Paeonia lactiflora extract.

[0189]

[0190] Comparative Example 1: Preparation of Composition D1

[0191] Ten parts by weight of Angelica gigas, five parts by weight of Cnidium officinale, and ten parts by weight of Paeonia lactiflora are taken and mixed uniformly. Ten times the amount of water is added, and the mixture is extracted with hot water at 95°C for four hours to obtain an extract. The extract is concentrated under reduced pressure until the solid content is 30% by weight, and then filtered to obtain a mixed concentrate. This concentrate is divided into a first mixed concentrate and a second mixed concentrate. Four times the amount of 95% ethanol is added to the second mixed concentrate, left at room temperature for 16 hours, and then centrifuged to obtain a third mixed precipitate from the mixed extract. The first mixed concentrate and the third mixed precipitate are mixed in a weight ratio of 6.0:4.0 to obtain Comparative Composition D1.

[0192]

[0193] Comparative Example 2: Preparation of Composition D2

[0194] 5 parts by weight of Angelica gigas, 8 parts by weight of Cnidium officinale, and 6.5 parts by weight of Paeonia lactiflora are taken and uniformly mixed. Ten times the amount of water is added, and the mixture is extracted with hot water at 95°C for 4 hours to obtain an extract. The extract is concentrated under reduced pressure until the solid content is 30% by weight, and then filtered to obtain a mixed concentrate. This concentrate is divided into a first mixed concentrate and a second mixed concentrate. Four times the amount of 95% ethanol is added to the second mixed concentrate, left at room temperature for 16 hours, and then centrifuged to obtain a third mixed precipitate from the mixed extract. The first mixed concentrate and the third mixed precipitate are mixed in a weight ratio of 6.0:4.0 to obtain comparative composition D2.

[0195]

[0196] Comparative Example 3: Preparation of Composition D3

[0197] 10 parts by weight of Angelica gigas are taken, 10 times the amount of water is added first, and the mixture is boiled at 95°C for 4 hours. Then, 5 times the amount of water is added and the mixture is boiled at 95°C for 2 hours. The extracts from both methods are combined and concentrated to a solid content of 30% by weight to obtain a hot water extract of Angelica gigas.

[0198] Take 5 parts by weight of Cnidium officinale, first add 10 times the amount of water, and decoct at 95°C for 4 hours. Then, add 5 times the amount of water and decoct at 95°C for 2 hours. Combine both extracts and concentrate to obtain a hot water extract of Cnidium officinale with a solid content of 30% by weight.

[0199] 10 parts by weight of white peony root are taken, 10 times the amount of water is added first, and the mixture is boiled at 95°C for 4 hours. Then, 5 times the amount of water is added and the mixture is boiled at 95°C for 2 hours. The extracts from both sides are combined and concentrated to a solid content of 30% by weight to obtain a hot water extract of white peony root.

[0200] Comparative composition D3 is obtained by taking 10.20 parts of Angelica gigas extract, 3.00 parts of Cnidium officinale extract, and 4.31 parts of Paeonia lactiflora extract.

[0201]

[0202] Experimental research

[0203] Example 8: Study on the immune-enhancing effect of Composition C1 of the present invention

[0204] Sixty male Kunming mice weighing 18–22 g were acclimatized for 5 days in an SPF environment (room temperature 22–26°C, relative humidity 60–70%, light-dark cycle 12 hours) and then divided into six groups: distilled water group (distilled water administered via gastric tube feeding), model group, Samultang group (2.5 g / kg), low-dose composition group (1.12 g / kg), medium-dose group (2.24 g / kg), and high-dose group (6.72 g / kg). The dosage of the composition in the low-dose, medium-dose, and high-dose groups corresponded to 5, 10, and 30 times the recommended human dosage (based on 60 kg), respectively. Ten mice were assigned to each group, and a daily dose of 0.1 ml / 10 g was administered orally for 30 days. An immunodeficiency animal model was established by administering 0.35 mg / kg of dexamethasone once a day in the morning by gastric tube feeding to the groups other than the distilled water group for 30 days.

[0205] Research Results:

[0206] (1) Effect of the present composition C1 on the immune organ index of immunocompromised mice

[0207] Compared to the distilled water group, the model group showed a significant decrease in spleen weight (P<0.001) and a significant decrease in spleen index (P<0.001). When compared to the dexamethasone model group, the Samultang group showed a significant increase in spleen weight (P<0.01) and a significant decrease in spleen index (P<0.05). In the high-dose group, spleen weight significantly increased (P<0.01) and the spleen index also significantly increased (P<0.05). When compared to the Samultang group, the low-dose, medium-dose, and high-dose groups showed similar effects to the Samultang group in terms of thymus weight, thymus index, spleen weight, and spleen index. For details, refer to Table 1 (Table 1. Effects of composition on thymus index and spleen index in mice (x±s)).

[0208] Animals by Group Thymus (g) Spleen (g) Thymus Index (mg / g) Spleen Index (mg / g) Distilled Water Group 100.04±0.02 0.13±0.04 0.89±0.39 2.88±0.85 Model Group 100.03±0.01 0.05±0.01 *** 0.65±0.261.16±0.21 *** Samultanggun 100.03±0.01 0.07±0.01 △△ 0.66±0.14 1.63±0.32 △ Composition Low Dose Group 100.03±0.01 0.06±0.01 0.68±0.12 1.33±0.29 Composition Medium Dose Group 100.03±0.01 0.06±0.02 0.80±0.14 1.40±0.41 Composition High Dose Group 100.03±0.01 0.07±0.01 △△ 0.61±0.26 1.59±0.32 △

[0209] Note: *P<0.05, **P<0.01, ***P<0.001 compared to distilled water group; △P<0.05, △△P<0.01, △△△P<0.001 compared to model group

[0210]

[0211] (2) Effect of the present composition C1 on cytokine levels in immunocompromised mice

[0212] Compared to the distilled water group, the dexamethasone model group showed a significant decrease in IL-6 levels (P<0.001) and a significant increase in IL-10 and TNF-α levels (P<0.001, P<0.001). Compared to the dexamethasone model group, the Samultang group showed a significant decrease in IL-10 and TNF-α levels (P<0.05, P<0.001), and the low-dose group showed a significant decrease in IL-10 and TNF-α levels (P<0.001, P<0.001). The medium-dose group also showed a significant decrease in IL-10 and TNF-α levels (P<0.001, P<0.001). The high-dose group showed a significant increase in IL-6 levels (P<0.001) and a significant decrease in IL-10 and TNF-α levels (P<0.001, P<0.001). Compared to the Samultang group, IL-6 levels significantly increased in the high-dose group (P<0.01). The low-dose and medium-dose groups showed similar effects to the Samultang group regarding IL-6 levels. The low-dose, medium-dose, and high-dose groups showed similar effects to the Samultang group regarding IL-10 levels. TNF-α levels significantly decreased in the low-dose group (P<0.05). The medium-dose and high-dose groups showed similar effects to the Samultang group regarding TNF-α levels. For details, refer to Table 2 (Table 2. Effects of Composition C1 on IL-6, IL-10, and TNF-α in mice (x±s)).

[0213] Number of Animals by Group IL-6 (pg / mL) IL-10 (pg / mL) TNF-α (pg / mL) Distilled Water Group 10 63.66±4.12 365.85±29.23 240.66±22.64 Model Group 10 54.22±1.93 *** 420.60±49.72 *** 281.71±12.83 *** Samultang-gun 1056.30±4.04387.07±39.22 △ 239.11±20.91 △△△ Composition Low dose group 105.02±4.70365.47±18.19 △△△ 222.71±12.76 △△△#Composition Medium Dose Group 1057.45±3.02361.28±21.11 △△△ 234.18±12.19 △△△ Composition High dosage group 1061.22±3.31 △△△## 366.95±20.78 △△△ 240.85±11.92 △△△

[0214] Note: Compared to distilled water group *P<0.05, **P<0.01, ***P<0.001; compared to model group △P<0.05, △△P<0.01, △△△P<0.001; compared to Samultang group #P<0.05, ##P<0.01, ###P<0.001

[0215]

[0216] (3) Effects of the present composition C1 on NK cell activity and lymphocyte proliferation in immunocompromised mice

[0217] Compared to the distilled water group, NK cell activity and lymphocyte proliferation in the model group were significantly decreased (P<0.001, P<0.01). Compared to the model group, NK cell activity and lymphocyte proliferation in the Samultang group were significantly increased (P<0.05, P<0.05). NK cell activity significantly increased in the low-dose group (P<0.001), and also significantly increased in the medium-dose group (P<0.001); meanwhile, both NK cell activity and lymphocyte proliferation significantly increased in the high-dose group (P<0.05, P<0.01). Compared to the Samultang group, the low-dose, medium-dose, and high-dose groups showed similar effects on NK cell activity. For details, refer to Table 3 (Table 3. Effects of Composition C1 on NK cell activity and lymphocyte proliferation in mice (x±s)).

[0218] Number of Animals by Group NK Cell Activity (%) Lymphocyte Proliferation Distilled Water Group 10 4 2.19±12.76 0.04±0.01 Dexamethasone Model Group 10 16.71±3.33 *** 0.02±0.00 ** Samultang-gun 1032.66±5.52 △△△ 0.04±0.01 △Composition low dose group 1036.46±8.70 △△△ 0.03±0.01 composition medium dosage group 1038.03±8.11 △△△ 0.03±0.01 composition high dose group 1039.14±6.44 △△△ 0.05±0.01 △△

[0219] Note: *P<0.05, **P<0.01, ***P<0.001 compared to distilled water group; △P<0.05, △△P<0.01, △△△P<0.001 compared to model group

[0220]

[0221] Example 9. Effect of Composition C1 on the PI3K-Akt signaling pathway in immunocompromised mice

[0222] Thirty male Kunming mice weighing 18–22 g were acclimatized for 5 days in an SPF environment (room temperature 22–26°C, relative humidity 60–70%, light-dark cycle 12 hours) and then assigned 10 mice each to three groups: a distilled water group (distilled water administered via gastric tube feeding), a model group, and a high-dose composition group (6.72 g / kg). A daily dose of 0.1 ml / 10 g was administered orally for 30 days. An immunodeficiency animal model was established by administering 0.35 mg / kg of dexamethasone via gastric tube feeding once every morning for 30 days to the groups other than the distilled water group.

[0223] Research Results: Compared to the normal group, the P-PI3K / PI3K and P-Akt / Akt ratios were significantly decreased in the model group (P<0.01, P<0.001). Compared to the model group, the P-PI3K / PI3K and P-Akt / Akt ratios were significantly increased in the high-dose group (P<0.01, P<0.001). For details, refer to Table 4 and Figure 1 (WB band (Western blot) experiment, which detects protein expression levels in cell or tissue extracts using immunological detection technology). (Table 4. P-PI3K / PI3K and P-Akt / Akt ratios in mouse spleen tissue by group)

[0224] Number of animals by group P-PI3K / PI3KP-AKT / AKT distilled water group 30.94±0.01 1.16±0.02 Model group 30.56±0.02 *** 0.55±0.02 △△ Composition High dosage group 30.89±0.04 * 0.95±0.02 △

[0225] Note: *P<0.05, **P<0.01, ***P<0.001 compared to distilled water group; △P<0.05, △△P<0.01, △△△P<0.001 compared to model group.

[0226]

[0227] Example 10: Effect of Composition C1 on Hematopoietic Function in Immunodeficient Mice

[0228] Sixty male Kunming mice weighing 18–22 g were acclimatized for 5 days in an SPF environment (room temperature 22–26°C, relative humidity 60–70%, light-dark cycle 12 hours) and then divided into six groups: a distilled water group (distilled water administered via gastric tube feeding), a model group, a Samultang group (2.5 g / kg), a low-dose composition group (1.12 g / kg), a medium-dose group (2.24 g / kg), and a high-dose group (6.72 g / kg). The dosage of the composition in the low-dose, medium-dose, and high-dose groups corresponded to 5, 10, and 30 times the recommended human dosage (based on 60 kg), respectively. Ten mice were assigned to each group, and a daily dose of 0.1 ml / 10 g was administered orally for 30 days. An immunosuppressed animal model was established by administering 80 mg / kg of cyclophosphamide via intraperitoneal injection (injection dose 0.1 ml / 10 g) to mice in each group, excluding the distilled water group, for two consecutive days on days 12 and 13.

[0229] Research Results: Serum IL-3 levels in model group mice were significantly decreased compared to the distilled water group (P<0.01). Compared to the model group, serum IL-3 levels in mice of the Samultang group and the high-dose composition group were significantly increased (P<0.05, P<0.05). Serum TNF-α levels in model group mice were significantly increased compared to the distilled water group (P<0.001). Compared to the model group, serum TNF-α levels in mice of the Samultang group, the medium-dose composition group, and the high-dose composition group were significantly decreased (P<0.05, P<0.05, P<0.001). Compared to the distilled water group, serum G-CSF levels in model group mice were significantly decreased (P<0.001). Compared to the model group, serum granulocyte colony-stimulating factor (G-CSF) levels in mice of the Samultang, medium-dose, and high-dose groups significantly increased (P<0.05, P<0.05, P<0.01). Compared to the distilled water group, serum granulocyte-macrophage colony-stimulating factor (GM-CSF) levels in mice of the model group significantly increased (P<0.001). Compared to the model group, serum GM-CSF levels in the Samultang, medium-dose, and high-dose groups significantly decreased (P<0.01, P<0.05, P<0.05). Compared to the Samultang group, serum G-CSF levels in the medium-dose and high-dose groups were similar to those of the Samultang group. For details, refer to Table 5 (Table 5. Effects of Composition C1 on mouse serum IL-3, TNF-α, G-CSF, and GM-CSF levels (X±s, n=10))

[0230] Animals by Group IL-3 (pg / mL) TNF-α (pg / mL) G-CSF (μg / mL) GM-CSF (μg / mL) Distilled Water Group 10 80.33±4.77 36.88±4.07 480.27±20.74 6.86±0.3 Model Group 10 71.57±5.25**4 5.26±7.06***4 30.76±14.90***6.15±0.32*** Samultang Group 10 78.38±5.20 △ 39.74±5.28△ 456.41±26.07 △ 6.62±0.42 △△ Composition Low Dose Group 107 4.44±7.30 43.06±4.29 44 3.74±27.8 6.34±0.4 Composition Medium Dose Group 107 5.58±4.85 40.09±3.76 △ 454.87±31.52 △ 6.5±0.45 △ Composition High dosage group 1077.61±7.28 △ 37.85±4.23 △△△ 460.94±26.03 △△ 6.58±0.37 △

[0231] Note: *P<0.05, **P<0.01, ***P<0.001 compared to distilled water group; △P<0.05, △△P<0.01, △△△P<0.001 compared to model group

[0232]

[0233] Example 11: Effect of Composition C1 on the spleen AMPK / mTOR signaling pathway in immunocompromised mice

[0234] Thirty male Kunming mice weighing 18–22 g were acclimatized for 5 days in an SPF environment (room temperature 22–26°C, relative humidity 60–70%, light-dark cycle 12 hours) and then assigned 10 mice each to three groups: a distilled water group (distilled water administered via gastric tube feeding), a model group, and a high-dose composition group (6.72 g / kg). A daily dose of 0.1 ml / 10 g was administered orally for 30 days. An immunodeficiency animal model was established by administering 0.35 mg / kg of dexamethasone via gastric tube feeding once every morning for 30 days to the groups other than the distilled water group.

[0235] Research Results: Compared to the distilled water group, the P-AMPK / AMPK ratio in model group mice significantly decreased (P<0.001), and the P-mTOR / mTOR ratio significantly increased (P<0.05). In the high-dose group, compared to the model group, the P-AMPK / AMPK ratio significantly increased (P<0.05), and the P-mTOR / mTOR ratio significantly decreased (P<0.05). For details, refer to Table 6 and Figure 2 (Table 6. P-PI3K / PI3K and P-Akt / Akt ratios in mouse spleen tissue by group).

[0236] Number of animals by group P-AMPK / AMPKP-mTOR / mTOR Distilled water group 31.18±0.13 0.65±0.12 Model group 30.53±0.05 *** 1.12±0.25 * Composition High dosage group 30.74±0.03 △ 0.74±0.12 △△

[0237] Note: *P<0.05, **P<0.01, ***P<0.001 compared to distilled water group; △P<0.05, △△P<0.01, △△△P<0.001 compared to model group

[0238]

[0239] Example 12: Effect on lymphocyte proliferation in the spleen

[0240] Mouse spleens were excised and pulverized under sterile conditions, and splenocytes were extracted. A 96-well plate was prepared, and 1 × 10⁶ cells were placed in each well. 6Cells were inoculated at a concentration of µg / ml. Concanavalin A (Con A) was used as a positive control. Two replicate wells were assigned to each group, and each sample was diluted with cell culture medium to a final concentration of 500 μg / ml. The cells were treated with the diluted samples and incubated for 48 hours at 37°C under 5% CO₂ conditions. After incubation, the cells were treated with WST-8 (cell viability test kit) and incubated in a dark room for 4 hours at 37°C under 5% CO₂ conditions. After incubation, absorbance was measured at 450 nm using an ELISA reader.

[0241] Research Results: The composition disclosed herein exhibited a superior spleen cell proliferation rate compared to Comparative Example compositions D1 to D3. In particular, the extract obtained by mixing Angelica gigas, Cnidium officinale, and Paeonia lactiflora according to the preparation method disclosed herein showed a more superior immune-enhancing effect compared to the mixed extraction method and the conventional hot water extraction method. Furthermore, the composition disclosed herein demonstrated superior spleen cell proliferation ability compared to Samultang, and exhibited a powerful immune-enhancing effect despite not containing Rehmannia glutinosa, unlike Samultang. For details, refer to Table 7 (Table 7. Effect on Spleen Cell Proliferation).

[0242] Cell proliferation rate by group Con A Control Group 100% Present Composition C 1122% Present Composition C 5108.5% Comparative Example D 193.5% Comparative Example D 297.5% Comparative Example D 3100.5% Samultang Group 98.5%

Claims

1. A method for preparing a composition for enhancing immunity, comprising the step of taking medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora in a weight ratio of 1 to 10:1 to 10:1 to 10:1, preferably 10:4 to 8:10, more preferably 10:5:10; 1 to 10 parts by weight of Angelica gigas are taken and decocted with water to extract, and the Angelica gigas hot water extract is concentrated. The hot water concentrate is divided into a first Angelica gigas hot water concentrate and a second Angelica gigas hot water concentrate so that the weight ratio of the first and second hot water concentrates is 1 to 10:10 to 1. 95 volume% of ethanol is added to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 50 to 90 volume%, after which an alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours. Then, the alcohol precipitate is concentrated again until the solid content reaches 30 weight% to obtain an Angelica gigas alcohol precipitate. The first Angelica gigas hot water concentrate and the Angelica gigas alcohol precipitate are mixed in a weight ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0 to obtain an Angelica gigas extract. The obtaining step; 1 to 10 parts by weight of Cnidium officinale are taken and decocted with water to extract the extract, and the hot water extract of Cnidium officinale is concentrated. The hot water concentrate is divided into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1. Then, 95 volume% of ethanol is added to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 50 to 90 volume%, after which an alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours. Then, the alcohol precipitate is concentrated again until the solid content reaches 30 weight% to obtain an alcohol precipitate of Cnidium officinale. The first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0 to obtain a Cnidium officinale extract. The obtaining step; A step of extracting 1 to 10 parts by weight of white peony root by boiling it in water, and concentrating the hot water extract of white peony root to obtain a white peony root extract; A method comprising the step of obtaining the immune-enhancing composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 1 to 10:1 to 10:1 to 10, preferably 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, preferably in the specified weight ratio.

2. In claim 1, 1 to 10 parts by weight of Angelica gigas are taken, and first, 10 times the amount of water (v / w) is added and boiled at 95°C for 4 hours, then 5 times the amount of water (v / w) is added and boiled at 95°C for 2 hours, and the extracts from both are combined and concentrated to obtain a hot water concentrate of Angelica gigas by a solid content of 30% by weight, and the hot water concentrate of Angelica gigas is divided into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas so that the weight ratio of the first hot water concentrate of Angelica gigas and the second hot water concentrate of Angelica gigas is 1 to 10:10 to 1, and 95% by volume of ethanol is added to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 65% by volume, then an alcohol precipitation treatment is performed, the lower precipitate is taken and left at room temperature for at least 12 hours, and then again until the solid content of the alcohol precipitate reaches 30% by weight. A step of concentrating to obtain an Angelica alcohol precipitate, and mixing the first Angelica hot water concentrate and the Angelica alcohol precipitate in a weight ratio of 6.0:4.0 to obtain an Angelica extract; Take 1 to 10 parts by weight of Cnidium officinale, first add 10 times the amount of water (v / w) and decoct at 95°C for 4 hours, then add 5 times the amount of water (v / w) and decoct at 95°C for 2 hours, combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale, divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1, add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 65% by volume, then perform an alcohol precipitation treatment, take the lower precipitate and leave it at room temperature for at least 12 hours, then concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain the Cnidium officinale alcohol precipitate A step of obtaining, and mixing the first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale in a weight ratio of 6:4 to obtain a Cnidium officinale extract; A step of taking 1 to 10 parts by weight of white peony, first adding 10 v / w of water and boiling at 95°C for 4 hours, then adding 5 v / w of water and boiling at 95°C for 2 hours, combining both extracts and concentrating to a solid content of 30 wt% to obtain a white peony extract; A method comprising the step of obtaining the above-mentioned immune-enhancing composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 2~8:2~5:2~9, 4~7:1~4:4~7, or 5~6:2~3:5~6.

3. An immune-boosting composition prepared from medicinal materials including Angelica gigas, Cnidium officinale, and Paeonia lactiflora, wherein the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 1 to 10:1 to 10:1 to 10, preferably 10:4 to 8:10, and more preferably 10:5:

10.

4. A composition for enhancing immunity according to claim 3, wherein the composition comprises extracts prepared from medicinal materials of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, and the weight ratio of the Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract is 1 to 10:1 to 10:1 to 10, preferably 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, more preferably 5.6:2.28:5.56, 2.8:2.28:5.56, 8.41:2.28:2.56, 5.6:2.28:2.78, 5.6:2.28:8.34, 5.6:4.56:2.78, or 2.8:4.56:5.

56.

5. In claim 3 or 4, 1 to 10 parts by weight of Angelica gigas are taken and extracted by boiling with water, then the Angelica gigas hot water extract is concentrated, the hot water concentrate is divided into Angelica gigas first hot water concentrate and Angelica gigas second hot water concentrate so that the weight ratio of the first hot water concentrate to the second hot water concentrate is 1 to 10:10 to 1, 95 volume% of ethanol is added to the second hot water concentrate to adjust the ethanol concentration of the extract to 50 to 90 volume%, and then an alcohol precipitation treatment is performed, the lower precipitate after alcohol precipitation is taken and left at room temperature for at least 12 hours, then concentrated again until the solid content of the alcohol precipitate becomes 30 weight% to obtain an Angelica gigas alcohol precipitate, and the first hot water concentrate and the Angelica gigas alcohol precipitate are mixed in a ratio of 1 to 10:10 to 1, preferably A step of obtaining Angelica extract by mixing in a weight ratio of 6.0~7.0:3.0~4.0; 1 to 10 parts by weight of Cnidium officinale are taken and decocted with water to extract the extract, and the hot water extract of Cnidium officinale is concentrated. The hot water concentrate is divided into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1. Then, 95 volume% of ethanol is added to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 50 to 90 volume%, after which an alcohol precipitation treatment is performed. After alcohol precipitation, the lower precipitate is taken and left at room temperature for at least 12 hours. Then, the alcohol precipitate is concentrated again until the solid content reaches 30 weight% to obtain an alcohol precipitate of Cnidium officinale. The first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale are mixed in a weight ratio of 1 to 10:10 to 1, preferably 6.0 to 7.0:3.0 to 4.0 to obtain a Cnidium officinale extract. The obtaining step; A step of extracting 1 to 10 parts by weight of white peony root by boiling it in water, and concentrating the hot water extract of white peony root to obtain a white peony root extract; An immunity-enhancing composition prepared by a method comprising the step of obtaining a composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 1 to 10:1 to 10:1 to 10, preferably 2 to 8:2 to 5:2 to 9, 4 to 7:1 to 4:4 to 7, or 5 to 6:2 to 3:5 to 6, preferably in a specified weight ratio.

6. In claim 5, 1 to 10 parts by weight of Angelica gigas are taken, first 10 times the amount of water (v / w) is added and boiled at 95°C for 4 hours, then 5 times the amount of water (v / w) is added and boiled at 95°C for 2 hours, the extracts from both are combined and concentrated to obtain a hot water concentrate of Angelica gigas, the hot water concentrate of Angelica gigas is divided into a first hot water concentrate of Angelica gigas and a second hot water concentrate of Angelica gigas, such that the weight ratio of the first hot water concentrate of Angelica gigas to the second hot water concentrate of Angelica gigas is 1 to 10:10 to 1, 95 volume% of ethanol is added to the second hot water concentrate of Angelica gigas to adjust the ethanol concentration of the extract to 65 volume%, then an alcohol precipitation treatment is performed, the lower precipitate is taken and left at room temperature for at least 12 hours, and then again until the solid content of the alcohol precipitate reaches 30 weight% A step of concentrating to obtain an Angelica alcohol precipitate, and mixing the first Angelica hot water concentrate and the Angelica alcohol precipitate in a weight ratio of 6.0:4.0 to obtain an Angelica extract; Take 1 to 10 parts by weight of Cnidium officinale, first add 10 times the amount of water (v / w) and decoct at 95°C for 4 hours, then add 5 times the amount of water (v / w) and decoct at 95°C for 2 hours, combine both extracts and concentrate until the solid content reaches 30% by weight to obtain a hot water concentrate of Cnidium officinale, divide the hot water concentrate of Cnidium officinale into a first hot water concentrate of Cnidium officinale and a second hot water concentrate of Cnidium officinale so that the weight ratio of the first hot water concentrate to the second hot water concentrate of Cnidium officinale is 1 to 10:10 to 1, add 95% by volume of ethanol to the second hot water concentrate of Cnidium officinale to adjust the ethanol concentration of the extract to 65% by volume, then perform an alcohol precipitation treatment, take the lower precipitate and leave it at room temperature for at least 12 hours, then concentrate again until the solid content of the alcohol precipitate reaches 30% by weight to obtain the Cnidium officinale alcohol precipitate A step of obtaining, and mixing the first hot water concentrate of Cnidium officinale and the alcohol precipitate of Cnidium officinale in a weight ratio of 6.0:4.0 to obtain a Cnidium officinale extract; A step of taking 1 to 10 parts by weight of white peony, first adding 10 v / w of water and boiling at 95°C for 4 hours, then adding 5 v / w of water and boiling at 95°C for 2 hours, combining both extracts and concentrating to a solid content of 30 wt% to obtain a white peony extract; An immunity-enhancing composition prepared by a method comprising the step of obtaining the composition by mixing the above-mentioned Angelica gigas extract, Cnidium officinale extract, and Paeonia lactiflora extract in a weight ratio of 2~8:2~5:2~9, 4~7:1~4:4~7, or 5~6:2~3:5~6.

7. An immunity-boosting composition according to claim 3 or 4, prepared with Angelica gigas, Cnidium officinale, and Paeonia lactiflora, wherein the weight ratio of Angelica gigas, Cnidium officinale, and Paeonia lactiflora is 1 to 10:1 to 10:1 to 10, preferably 10:4 to 8:10, more preferably 10:5:

10.

8. A herbal medicine preparation comprising a composition according to any one of paragraphs 3 to 7 and a pharmaceutically acceptable excipient, wherein the herbal medicine preparation is prepared in the form of an ointment, an oral liquid preparation, a decoction, a tea, granules, pills, powder, tablets, or capsules.

9. Use for preparing a medicinal product of a composition according to any one of paragraphs 3 through 7 or a herbal medicine preparation according to paragraph 8, for the purpose of enhancing and / or regulating immunity, or treating and / or preventing deficiency of qi and blood.

10. Use for preparing a drug intended to regulate bone marrow hematopoietic function or the PI3K-Akt signaling pathway, of a composition according to any one of paragraphs 3 to 7 or a herbal medicine preparation according to paragraph 8.

11. Use of a composition according to any one of paragraphs 3 through 7 or a herbal medicine preparation according to paragraph 8 for enhancing immunity.

12. A method for enhancing immunity, comprising the step of administering to an individual a composition according to any one of paragraphs 3 to 7 or a herbal medicine preparation according to paragraph 8.

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