Antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use and its manufacturing method

Abstract

An antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use comprises deionized water, silver nanoparticles extracted from white fingerroot rhizomes, polyvinylpyrrolidone K-30, polyethylene glycol 200, sodium benzoate, glycerin, sodium propionate, HCO40 (Cremophor rh-40), 30% Sodium hydroxide and sodium hyaluronate. The invention applies Thai herbal innovation by chemically synthesizing silver nanoparticles from white fingerroot, producing particles with a size of 20–40 nanometers. These nanoparticles are incorporated into a formulation by mixing them into a solution containing the aforementioned ingredients, resulting in a clear liquid. The concentration is adjusted appropriately for use in cleaning medical instruments and general surfaces. The composition exhibits broad-spectrum antimicrobial activity, capable of eliminating a wide range of microorganisms, including viruses, fungi, common skin bacteria, multidrug-resistant hospital bacteria, anaerobic bacteria, and bacteria found in livestock environments.

Description

[0001] ANTIMICROBIAL SOLUTION FROM WHITE FINGERROOT-DERIVED SILVER

[0002] NANOPARTICLES FOR MEDICAL AND GENERAL USE AND ITS

[0003] MANUFACTURING METHOD

[0004] Field of the Invention

[0005] The present invention relates to the fields of nanotechnology and herbal pharmaceutical sciences, specifically to an antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use and its manufacturing method.

[0006] Background of the Invention

[0007] Nanotechnology involves materials with dimensions in the range of 1-100 nanometers, where the rearrangement of molecular structures enhances their physical and chemical properties. Various approaches exist for synthesizing nanomaterials, primarily categorized into top-down and bottom-up methods. The top-down nanofabrication method involves the creation of silver nanocrystals by reducing the size of bulk metal through physical and chemical processes. However, this approach often results in surface structure imperfections, which may limit the functional properties of the nanoparticles. Conversely, the bottom-up nanofabrication method involves assembling nanoparticles from atomic or molecular precursors. For instance, silver nanoparticles can be synthesized by reducing metal salts in suitable media using reducing agents such as borohydride, citrate, or ascorbate. This reduction process transforms silver ions (Ag+) into silver atoms (Ag°), leading to nanoparticle precipitation. When the size of these nanoparticles falls below the visible light wavelength, they exhibit a characteristic yellow hue. This method allows precise control over nanoparticle structure, yielding well-defined chemical and biological properties. In pursuit of an eco-friendly and cost-effective approach to synthesizing silver nanoparticles while maintaining their desirable properties, researchers have explored biological synthesis — also known as green synthesis. This method leverages biological agents such as bacteria, fungi, and plant-derived phytochemicals, which act as natural reducing and stabilizing agents. Specifically, plant-derived antioxidants and phytochemicals facilitate silver ion reduction, while nicotinamide adenine dinucleotide (NADH), generated during glycolysis, donates electrons to Ag+, further driving nanoparticle formation.

[0008] White fingerroot (Boesenbergia rotunda) is a perennial herbaceous plant characterized by cylindrical, tapering rhizomes measuring approximately 4-10 cm in length and 1-2 cm in width, with a light brown outer skin and a yellow, aromatic interior. Traditionally used in culinary and herbal medicine, white fingerroot exhibits various biological activities. Studies by Eng-Chong et al. have demonstrated its antimicrobial and antifungal properties, as well as its anti-inflammatory effects, making it a valuable component in traditional medicine formulations. Summary of the Invention

[0009] Polyethylene glycol 200 (PEG-200)

[0010] PEG-200 is a low-viscosity, clear, non-volatile liquid with an average molecular weight of approximately 190-210 g / mol. It exhibits excellent hygroscopic properties and is commonly used as a solvent and additive. PEG-200 is non-toxic to the respiratory and digestive systems and does not cause skin or eye irritation, making it a safe substance for various applications. Studies have demonstrated that PEG-200 serves as an effective solvent and coating agent in the synthesis of silver nanoparticles from white fingerroot. This process results in nanoparticles with a controlled size range of approximately 10-30 nanometers, stabilized by PEG-200. The use of PEG-200 in nanoparticle synthesis enhances particle stability while allowing precise control over particle size. Additionally, its hydrophilic and water-soluble nature facilitates the formation of well -dispersed silver nanoparticle solutions with improved flow properties.

[0011] Polyvinylpyrrolidone K-30 (PVP K-30)

[0012] PVP K-30, a positively charged polymer, is often used in combination with herbal extracts containing antioxidant compounds such as flavonoids. It plays a crucial role in coprecipitation processes utilizing supercritical antisolvent techniques, preventing particle agglomeration and precipitation. In the synthesis of silver nanoparticles from white fingerroot, PVP K-30 enhances particle dispersion in solution. When used alongside PEG-200, PVP K-30 provides complementary benefits, further improving nanoparticle stability, dispersion, and safety. Specifically, PVP K-30 forms a protective coating around nanoparticles, preventing aggregation. The combination of PEG-200 and PVP K-30 enables better control over nanoparticle size and distribution. Optimal stabilization is achieved when PVP K-30 is used in concentrations ranging from 0.1-1% by weight of the solution.

[0013] The dilution of a high-concentration solution containing PVP K-30 and PEG 200 with deionized water at a ratio of 1 : 10 to 1 :50 is feasible. Dilution with deionized water effectively reduces the concentration of white fingerroot-derived silver nanoparticles while maintaining particle stability through the coating effect of PVP K-30 and PEG 200, without requiring additional stirring. This process yields a silver nanoparticle solution with a lower concentration while preserving particle stability. The ratio of PEG 200 to PVP K-30 can influence the particle size, dispersion, and stability of the silver nanoparticles. An inappropriate ratio may lead to reduced particle stability. Both PVP K-30 and PEG 200 enhance the solubility of flavonoids by facilitating solid dispersion. PVP K-30, which carries a positive charge, can interact with positively charged herbal extracts that exhibit antioxidant activity, such as flavonoids, thereby improving their solubility and dispersion. Preventing the aggregation of white fingerroot-derived silver nanoparticles is critical, as aggregation increases particle size, which can negatively impact their properties. Smaller particles offer a higher surface area-to-volume ratio, enabling them to penetrate viral, fungal, and bacterial membranes more effectively. The surface coating with PVP K-30 plays a crucial role in maintaining the particle size and properties of the silver nanoparticles. PVP K-30 coats the particles by adhering its pyrrolidone groups to the particle surface, generating repulsion between particles and preventing aggregation, thereby preserving the desired size and characteristics.

[0014] For a silver nanoparticle solution with a concentration of 100 mg / L, PVP K-30 should be used in an amount of approximately 1-10 g per liter of solution to achieve a PVP K-30 concentration of 0.1-1% by weight. Similarly, for a silver nanoparticle solution derived from white fingerroot with a concentration of 100 mg / L, PEG 200 should be used in an amount of approximately 1-10 g per liter of solution to achieve a PEG 200 concentration of 0.1-1% by weight. This ensures that PEG 200 functions effectively as a dispersing agent, enhancing the stability and solubility of the silver nanoparticles.

[0015] Sodium Benzoate

[0016] Sodium benzoate plays a crucial role in enhancing the efficiency of silver nanoparticles by acting as a stabilizer, improving their stability through surface coating to prevent aggregation and precipitation. Additionally, it regulates the size and distribution of white fingerroot-derived silver nanoparticles, ensuring the formation of uniformly small particles. Sodium benzoate also exhibits antioxidant properties, reducing oxidation reactions that may occur on the surface of these nanoparticles, thereby maintaining their stability in solutions. Furthermore, it mitigates toxicity by inhibiting the release of Ag+ions, which could otherwise lead to instability in the system. The combination of sodium benzoate with plant extracts or other antioxidants further enhances control over size, dispersion, and stability of silver nanoparticles, making it particularly suitable for biological applications and medical use where high safety standards are required.

[0017] Glycerin

[0018] Glycerin significantly enhances the efficiency and stability of silver nanoparticles by functioning as both a stabilizer and a viscosity-enhancing agent, preventing aggregation and precipitation in solutions containing silver nanoparticles from white fingerroot. Due to its high moisture-retention capability, glycerin helps maintain an optimal environment for these nanoparticles, ensuring uniform size and distribution. Additionally, it is highly compatible with biological substances and can work synergistically with antioxidants or other stabilizers to further improve nanoparticle dispersion and stability. Glycerin also reduces oxidation on the nanoparticle surface, contributing to greater overall stability. Its non-toxic and biocompatible nature makes it well-suited for applications in biological systems, cosmeceuticals, and medical fields that require high safety standards.

[0019] Sodium Hyaluronate

[0020] Sodium hyaluronate is a negatively charged compound with high water-retention capacity, effectively enhancing the performance and stability of silver nanoparticles. Sodium hyaluronate functions as a surface-coating agent for white fingerroot-derived silver nanoparticles, thereby preventing aggregation and sedimentation. This leads to smaller particle size and more uniform dispersion. Sodium hyaluronate also helps reduce surface oxidation of the silver nanoparticles, resulting in improved stability of the particles in solution. Moreover, sodium hyaluronate contributes to reducing the cytotoxicity of silver nanoparticles by limiting the release of Ag+ions, which are highly toxic. Its favorable biological properties — including high biocompatibility and efficient drug delivery capabilities — make sodium hyaluronate particularly suitable for the incorporation of white fingerroot-derived silver nanoparticles in medical, cosmeceutical, and biological applications. It enhances delivery efficiency, safety, and functional performance of the nanoparticles in such systems.

[0021] The antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use comprises deionized water, silver nanoparticles extracted from white fingerroot, polyvinylpyrrolidone K-30, polyethylene glycol 200, sodium benzoate, glycerin, and sodium hyaluronate. Fragrance additives may also be incorporated as per market preferences, particularly in antimicrobial solutions for general use.

[0022] The objective of this invention is to develop an antimicrobial solution from Thai herbal extract-derived silver nanoparticles for medical and general use. The formulation is designed to effectively eliminate viruses, bacteria, and fungi. In this invention, herbal extracts are employed as natural reducing agents to synthesize silver nanoparticles, which are subsequently incorporated into a stabilizing solution to ensure nanoparticle stability and user-friendly application. The antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use offers broad- spectrum antimicrobial efficacy, reducing the risk of infections caused by various pathogens. This includes efficacy against mutated strains of coronavirus (COVID-19). The white fingerroot-derived silver nanoparticles exhibit both antioxidant and potent antimicrobial activities, effectively neutralizing common bacteria, multi drug -resistant hospital bacteria, and fungi.

[0023] Brief Description of the Drawings

[0024] Figure 1 : The efficacy of biologically synthesized white fingerroot-derived silver nanoparticles at a concentration of 10 ppm. When diluted at a 1 : 10 ratio (resulting in a final concentration of 1 ppm), the nanoparticles exhibit 100% virucidal activity against SARS-CoV-2

[0025] Omicron subvariants BA.5 and BA.2.75.

[0026] Figure 2: The efficacy of biologically synthesized white fingerroot-derived silver nanoparticles at a concentration of 10 ppm. When diluted at a 1 : 10 ratio (resulting in a final concentration of 1 ppm), the nanoparticles exhibit 100% virucidal activity against SARS-CoV-2 Omicron subvariants BA.5 and BA.2.75.

[0027] Figure 3: The virucidal activity of white fingerroot-derived silver nanoparticles (MDlight) at a concentration of 0.5 ppm against porcine epidemic diarrhea virus (PEDV).

[0028] Figure 4: Evaluation of the antibacterial activity of white fingerroot-derived silver nanoparticles against E. coli ATCC 25922, S. aureus ATCC 25923, and S. epidermidis ATCC 12228.

[0029] Figure 5: Evaluation of the antibacterial activity of white fingerroot-derived silver nanoparticles against E. coli ATCC 25922, S. aureus ATCC 25923, and S. epidermidis ATCC 12228.

[0030] Figure 6: Evaluation of the antibacterial activity of white fingerroot-derived silver nanoparticles against E. coli ATCC 25922, S. aureus ATCC 25923, and S. epidermidis ATCC 12228.

[0031] Detailed Description of the Invention

[0032] An antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use comprises:

[0033] Deionized Water 90.81 - 94.262 % by weight

[0034] Biosynthesized Silver Nanoparticles 0.001 - 0.005 % by weight

[0035] Polyvinylpyrrolidone K-30 (PVP K-30) 0.32 - 0.65 % by weight

[0036] Polyethylene Glycol 200 (PEG-200) 0.47 - 0.59 % by weight

[0037] Sodium Benzoate % by weight

[0038] Glycerin 3.84 - 5.59 % by weight

[0039] Sodium Hyarulonate 0.008 - 0.02 % by weight

[0040] Sodium propionate 0.01 - 0.12 % by weight

[0041] HCO40 (Cremophor rh-40) 0.1 - 0.89 % by weight 30% Sodium hydroxide 0.03 - 0.46 % by weight

[0042] A Manufacturing Method of An Antimicrobial Solution from White Fingerroot- Derived Silver Nanoparticles for Medical and General Use

[0043] Synthesis of Silver Nanoparticles from White Fingerroot a. Preparing a reducing agent by washing 20 grams of white fingerroot rhizomes with distilled water, drying at room temperature, cutting into small pieces, and boiling the pieces in 100 milliliters of deionized water at 60°C using a heated magnetic stirrer for 30 minutes; filtering the resulting solution through Whatman No. 1 filter paper to remove solid residues; storing the filtrate at 4°C; and analyzing the protein content of the extract by measuring absorbance at 230 nanometers using a UV-Vis spectrophotometer; b. Synthesizing silver nanoparticles by mixing 5 milliliters of a silver nitrate (AgNCh, Sigma- Aldrich, USA) solution of 0.01-0.02 M with 25 milliliters of the reducing agent prepared in step a., adjusting the total volume to 100 milliliters using deionized water in a volumetric flask; heating the mixture at 50-60°C for 6-8 hours until a color change from colorless to reddish-brown is observed, indicating the formation of silver nanoparticles; cooling the reaction mixture to room temperature and centrifuging to collect the precipitated silver nanoparticles; washing the precipitate twice with deionized water; confirming the removal of residual nitrate ions by adding 1 milliliter of saturated ferrous sulfate solution to the supernatant from the second wash and carefully layering concentrated sulfuric acid, wherein the formation of a brown ring indicates the presence of nitrate ions; and redispersing the purified silver nanoparticles in white fingerroot extract to obtain a stable silver nanoparticle solution; c. Measuring the optical absorbance of the silver nanoparticles using a UV-Vis spectrophotometer at a resolution of 1 nanometer within the wavelength range of 300-800 nanometers to analyze the formation of silver nanoparticles;

[0044] Analyzing the morphology and particle size of the silver nanoparticles (AgNPs) using transmission electron microscopy (TEM) at an accelerating voltage of 200 kilovolts, wherein prior to analysis, the silver nanoparticle solution is subjected to sonication for 5 minutes. A droplet of the solution is then deposited onto a carbon-coated copper grid and subsequently vacuum-dried to remove moisture before TEM imaging is performed;

[0045] Analyzing the crystal structure of the silver nanoparticle powder using X-ray diffraction (XRD) at a 29 angle range of 10-60 degrees to investigate the crystallinity of the silver nanoparticles;

[0046] Recording the infrared spectrum in the range of 400-4000 cm1using a Fourier Transform Infrared Spectrometer (FTIR) to identify the molecular structures of organic compounds present in the reducing agent, including proteins and phytochemicals, which contribute to the stabilization of the silver nanoparticles; Evaluating the stability of the synthesized silver nanoparticles by subjecting the nanoparticles, stored for varying durations of 1, 7, 14, 30, 60, and 120 days after synthesis, to ultraviolet-visible (UV-Vis) spectrophotometric analysis to monitor changes in light absorbance, and measuring the particle size at each storage interval using transmission electron microscopy (TEM); d. Adding deionized water into a beaker, followed by adding glycerin, sodium hyaluronate, and polyethylene glycol; stirring the mixture at a temperature of 75-80°C to combine the components; mixing the solution at a speed of 300-500 rpm for 20-25 minutes until a clear solution is formed; and cooling the mixture to room temperature to obtain a colorless, transparent liquid; e. Adding polyvinylpyrrolidone K-30 to the solution prepared in step d., followed by mixing at a speed of 300-500 rpm for 5-10 minutes; subsequently adding sodium benzoate and continuing mixing at 300-500 rpm for an additional 5-1 0 minutes; then incorporating HCO-40 (Cremophor RH-40) and sodium propionate into the mixture, continuing to stir at 300-500 rpm for 5-1 0 minutes; adjusting the pH of the solution with 30% sodium hydroxide solution as necessary, and mixing thoroughly until a clear solution is obtained. f. Incorporating the silver nanoparticles prepared in step b. into the clear liquid solution obtained in step e., mixing the resulting mixture at a speed of 300-500 rpm for 5-10 minutes, and subsequently allowing the solution to stand at room temperature not exceeding 40°C.

[0047] The concentration of polymers used in the present invention, including glycerin, sodium hyaluronate, polyvinylpyrrolidone K-30, polyethylene glycol 200, and sodium benzoate, are preferably provided in a range of 0.1 - 3.0% by weight. Such concentration in this invention result in optimal antimicrobial efficacy.

[0048] Silver nanoparticles (AgNPs) have been extensively studied for their antimicrobial properties, with substantial medical evidence demonstrating their efficacy in eliminating bacteria, viruses, parasites, and fungi. Laboratory research has shown that AgNPs can effectively kill microorganisms at a minimum inhibitory concentration of less than 0.5 mg / L (ppm). The antimicrobial mechanism of silver nanoparticles involves their adhesion to the microbial cell surface, leading to the generation of reactive oxygen species that alter cellular structures and disrupt critical cellular processes. These include membrane permeability (in the case of viruses), the electrochemical potential between the intracellular and extracellular environment, and the formation of pores in the cell wall, ultimately causing ion leakage and cell death. Additionally, AgNPs accumulate outside the cells, releasing silver ions (Ag+) that penetrate the cells and interact with phosphorus compounds, DNA, or sulfur-containing thiol groups in proteins, thereby inhibiting genetic replication and protein function, leading to microbial inactivation.

[0049] Regarding safety and potential effects on human skin, studies indicate that AgNP absorption through the skin is minimal. Research has shown that silver nanoparticles of 10 nm in size are non-toxic to mouse skin and do not cause irritation in rabbits' skin or eyes. Similarly, AgNPs ranging from 10 to 20 nm in size do not cause irritation in guinea pig skin, confirming their safety for use in topical cosmetics, wound care, and sensitive skin applications. Further studies on human skin demonstrated that AgNPs at a concentration of 50 ppm do not penetrate intact skin. However, in inflamed skin, 0.2-2% of the applied AgNPs (equivalent to 0.002 - 0.02 ppm) can permeate the skin, but no cytotoxic effects were observed. Additionally, a study involving 30 bum patients, where AgNPs were applied to 12% of their total body surface area for over 28 days, found no abnormalities in blood parameters or biochemical markers indicating toxicity. For commercial applications, safety evaluations of AgNP-containing products at concentrations of 10 ppm and 32 ppm in healthy volunteers confirmed their safety. Oral exposure to silver nanoparticles in solution form was also found to be non-toxic. These findings collectively affirm the safety of AgNPs for dermatological applications.

[0050] Testing conducted by Institute of Biological Products, Department of Medical Sciences demonstrated that white fingerroot-derived silver nanoparticles at a concentration of 1 ppm achieved 100% inactivation of SARS-CoV-2 subvariants BA.5 and BA.2.75 (Figures 1-2). Additionally, these nanoparticles exhibit antimicrobial properties suitable for disinfecting medical equipment surfaces, floors, walls, furniture, handrails, and other surfaces. The application of nanotechnology to white fingerroot, a traditional Thai herbal remedy, provides an alternative to imported disinfectants, particularly those with potential toxicity upon skin or mucosal exposure.

[0051] Furthermore, white fingerroot-derived silver nanoparticles at a concentration of 0.5 ppm have demonstrated antiviral activity against the Porcine Epidemic Diarrhea Virus (PEDV), achieving 335-fold efficacy while remaining non-toxic to normal cells (Figure 3).

[0052] Additional evaluations conducted by the Faculty of Medical Technology at Mahidol University confirmed the bactericidal effectiveness of AgNPs against both common and drugresistant bacterial strains (Table 1). Similarly, the Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, validated the antifungal activity of biologically synthesized AgNPs from white fingerroot against various fungal species (Table 2). Further studies have assessed their effectiveness against anaerobic bacteria (Table 3) and other bacterial strains relevant to livestock industries (Table 4). Table 1 : The antibacterial efficacy of silver nanoparticles against common and multidrug-resistant hospital bacteria.

[0053] Source: Assoc. Prof. Sakda Yainoi, Faculty of Medical Technology, Mahidol University.

[0054] The research team submitted samples of the biosynthesized white fingerroot-derived silver nanoparticles (Bio-AgNP) to the National Institute of Health, Department of Medical Sciences, for evaluation of their antibacterial efficacy against A. coli ATCC 25922, S. aureus ATCC 25923, and S. epidermidis ATCC 12228. The results (Figures 4-6) indicated the following:

[0055] 1. The inhibition zone assay was conducted at a solution concentration of 200 mg / L.

[0056] 2. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were performed at a bacterial concentration of 1.5 x 106CFU / mL. The results demonstrated that the tested sample could reduce the bacterial count by approximately one million-fold (6 log reduction), achieving a 99.99% bactericidal effect.

[0057] 3. The lowest dilution titer for MIC and MBC determination was 1 :256, corresponding to a concentration of 0.78 mg / L. Table 2: The Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) values of the biosynthesized white fingerroot-derived silver nanoparticles (Bio-AgNP) against fungal pathogens.

[0058] Source: Kornvalee Meesilpavikkai, M.D., Department of Microbiology, Faculty of

[0059] Medicine, Chulalongkorn University.

[0060] Table 3: Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the biosynthesized white fingerroot-derived silver nanoparticles (Bio-AgNP) for the Elimination of Anaerobic Bacteria.

[0061] Table 4: The results of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the biosynthesized white fingerroot-derived silver nanoparticles on the antimicrobial activity against various bacteria, including those in livestock. The optimal concentration for medical instrument disinfectants ranges from 15-100 ppm (mg / L), and for surface disinfectants ranges from 1-20 ppm (mg / L).

[0062] MD Products and Services Co., Ltd. has developed a multi-purpose surface spray product utilizing the biosynthesized white fingerroot-derived silver nanoparticles. This formulation is free of alcohol yet exhibits potent antimicrobial properties, effectively eliminating Coronavirus-19, skin bacteria, and multidrug-resistant hospital bacteria. Additionally, it demonstrates antifungal activity. The white fingerroot-derived silver nanoparticles serve as an antimicrobial agent, preventing infections through contact by neutralizing viruses, bacteria, and fungi, including both drug-sensitive and drug-resistant strains. Unlike alcohol-based disinfectants, this formulation is non-flammable and does not cause any adverse skin reactions upon contact.

[0063] Best Mode of the Invention

[0064] As disclosed in the Detailed Description of the Invention.

Claims

Claims1. An antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use, comprising:Deionized Water 90.81 - 94.262 % by weightBiosynthesized Silver Nanoparticles 0.001 - 0.005 % by weight Polyvinylpyrrolidone K-30 (PVP K-30) 0.32 - 0.65 % by weight Polyethylene Glycol 200 (PEG-200) 0.47 - 0.59 % by weight Sodium Benzoate 1.75 - 2.11 % by weightGlycerin 3.84 - 5.59 % by weightSodium Hyarulonate 0.008 - 0.02 % by weightSodium propionate 0.02 - 0.12 % by weightHCO40 (Cremophor rh-40) 0.1 - 0.89 % by weight30% Sodium hydroxide 0.03 - 0.46 % by weight2. The manufacturing method of an antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use according to Claim 1, comprising the following steps: a. Preparing a white fingerroot extract by washing 20 grams of rhizome, boiling in 100 milliliters of water at 60°C for 30 minutes with heated magnetic stirring, and filtering the solution to remove solids using Whatman No. 1 filter paper, wherein the extract acts as a reducing and stabilizing agent for silver nanoparticle synthesis; b. Synthesizing silver nanoparticles by mixing a silver nitrate (AgNOs) solution having a concentration of 0.01-0.02 M with the white fingerroot extract at an appropriate ratio, and maintaining the mixture at a temperature of 50-60 °C for 6- 8 hours to facilitate a reduction reaction, thereby converting silver ions into silver nanoparticles, wherein the formation of the nanoparticles is indicated by a color change from clear to reddish-brown; c. Separating the precipitate using high-speed centrifugation, washing the precipitate with deionized water to remove residual substances, and characterizing the physical and chemical properties using analytical techniques, including UV-Vis spectroscopy for analyzing light absorption, transmission electron microscopy (TEM) for examining particle size and morphology, X-ray diffraction (XRD) for determining crystal structure, and Fourier-transform infrared spectroscopy (FTIR) for identifying chemical functional groups, wherein said characterization ensures quality control and stability of the synthesized silver nanoparticles;d. Adding deionized water into a beaker, adding 3.84-5.59% by weight of glycerin, 0.008-0.02% by weight of sodium hyaluronate, and 0.47-0.59% by weight of polyethylene glycol, stirring the mixture at 75-80°C, and mixing at a speed of SOO- SOO rpm for 20-25 minutes until a clear solution is obtained, followed by cooling the solution to room temperature to yield a colorless, clear solution; e. Adding polyvinylpyrrolidone K-30 in an amount of 0.32-0.65% by weight to the solution obtained from step d., and mixing at 300-500 rpm for 5-10 minutes; then adding sodium benzoate in an amount of 1.75-2.11% by weight and mixing at 300- 500 rpm for 5-10 minutes; subsequently adding HCO-40 (Cremophor RH-40) in an amount of 0.10-0.89% by weight and mixing at 300-500 rpm for 5-10 minutes; followed by the addition of sodium propionate in an amount of 0.01-0.12% by weight, mixing again at 300-500 rpm for 5-10 minutes; then adding 30% sodium hydroxide solution in an amount of 0.03-0.46% by weight and adjusting the pH as needed, mixing thoroughly; thereafter adding white fingerroot-derived silver nanoparticles in an amount of 0.001-0.005% by weight and mixing at 300-500 rpm for 5-10 minutes. The resulting solution is then allowed to stand at room temperature not exceeding 40°C until a clear liquid is obtained. Finally, adjust the concentration with deionized water to 1-100 ppm (mg / L) for use in surface cleaning applications.

3. The manufacturing method of an antimicrobial solution from white fingerroot-derived silver nanoparticles for medical and general use according to Claim 1, wherein the concentration of polymers used in the present invention, including glycerin, sodium hyaluronate, polyvinylpyrrolidone K-30, polyethylene glycol 200, and sodium benzoate, are preferably provided in a range of 0.1 - 3.0% by weight.

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