Stable synergistic insecticidal composition and process for the preparation thereof

Abstract

The present invention relates to a stable synergistic insecticidal composition comprising A) a compound of Formula I, i. e., N- [2, 6 -bis ( 1 -methylethyl ) -4-phenoxyphenyl) -N ' - ( 1, 1- dimethylethyl) thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, isomers, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives for effective control of various insects-pests and wide range of diseases caused by them. The present invention also relates to a process for preparation of such insecticidal composition for synergistic and efficacious control of various insects such as sucking insect-pest, boring insect-pest, etc.

Description

[0001] STABLE SYNERGISTIC INSECTICIDAL COMPOSITION AND PROCESS FOR THE PREPARATION THEREOF FIELD OF THE INVENTION

[0002] The present invention relates to a stable synergistic insecticidal composition. Particularly, the present invention relates to a stable synergistic insecticidal composition comprising A) a compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-( 1, 1-dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, isomers, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives for effective control of various insects-pests and wide range of diseases caused by them. The present invention also relates to a process for preparation of such insecticidal composition for synergistic and efficacious control of various insects such as sucking insect-pest, boring insect-pest, etc.

[0003] BACKGROUND OF THE INVENTION

[0004] Insecticides are substances used to control, repel, or kill insects that are considered harmful in various agricultural settings. The development and use of insecticides have played a significant role in enhancing agricultural productivity, protecting crops from insect damage, and consequently increasing food security.

[0005] Consistent use of insecticidal formulation leads to accumulated challenges of toxicity, ecological effect and resistance to specific target insect-pest population and its biodiversity. Further, the developing framework of insecticidal formulation after application in plants can basically influence their metabolism and productivity, requiring effective adversary of developing responses for ensure ideal crop yield and quality. Insect pest resistance has become a significant concern, severely undermining the effectiveness of traditional insecticidal formulations.

[0006] Further, commonly used insecticide formulations often have detrimental effects on plants and crops, leading to slow absorption and toxicity issues. Furthermore, the existing insecticidal compositions as realized in the earlier expressions frequently adversely affect non-target life formsand the climate, raising considerably significant issues connected with phytotoxicity, staining of leaves and different harms to plants.

[0007] Thus, recent research efforts in the field of entomology and agricultural sciences have focused on developing novel insecticides with specific action mechanisms, which minimize collateral damage to non-target organisms, including beneficial insects-pests.

[0008] State of art in insecticidal composition are tailored based on the active ingredients are used along with their intended applications. References can be made to CN102885038, which discloses the microemulsion pesticidal formulation consists of the following components in percentage by weight: 2.2 to 10 percent of original drug, 2.5 to 20 percent of cyclohexanone, 2 to 10 percent of nonylphenol polyoxyethylene ether, and water for supplementing; wherein the raw pesticide is one or two of beta-cypermethrin, beta-cyhalothrin, cyfluthrin, methomyl, carbosulfan, pyriproxyfen, fluacrypyrim, diafenthiuron, hexaflumuron, phoxim, abamectin and acetamiprid.

[0009] Further CN 103004825 discloses a pesticidal microcapsule suspension comprising a first active ingredient diafenthiuron 5-40%, a second active ingredient 10-75%, an auxiliary agent and 5-20% and water.

[0010] Conventionally, insect management relies on repeated applications of single-active insecticides or binary mixtures. However, continuous and indiscriminate use of insecticides having similar modes of action has resulted in several challenges, including:

[0011] • rapid development of insect resistance,

[0012] • resurgence of secondary pests,

[0013] • insufficient control of mixed insect populations, and

[0014] • reduced residual efficacy

[0015] Modern integrated insect-pest management therefore demands insecticidal compositions capable of providing broad-spectrum control, resistance management, long residual activity, and reduced application frequency. However, existing insecticidal combinations show adverse effect on plants and crops such as discoloration of plant leaves, resistance against insect-pests complex and are ineffective against control of major stages of insect-pests complexes.Thus, there is a need to overcome these challenges mentioned herein above. In view of the same, the present objectives to develop a stable synergistic insecticidal composition having an enhanced efficacy, reduce the likelihood of resistance development, reduce toxicity, increase residual activity, reduced dosages, offers broader crop protection, improved and healthy foliage, improved crop yield, saves labour, mitigate environmental impact and economical and is yet cost effective to the end user.

[0016] The stable synergistic insecticidal composition of the present invention will thereby target multiple biological pathways in insects, thereby increasing mortality rates and reduces the probability of resistance and where actives in the composition are mutually compatible and also resulting in stable synergistic composition also.

[0017] Therefore, the aim of the present invention is to provide a stable synergistic insecticidal composition that addresses these needs by providing a novel stable synergistic insecticidal composition that combines active ingredients and that too in specific weight percentage ranges. Composition of present invention Combining active ingredients possessing distinct and complementary modes of action represents an effective strategy for achieving synergistic efficacy while delaying resistance development.

[0018] The inventor of present invention has surprisingly found that the combination of Compound of formula I, Isocycloseram, and Pyriproxyfen produces an unexpected technical effect resulting from:

[0019] 1. Metabolic inhibition and contact toxicity provided by Compound of formula I;

[0020] 2. Neurotoxicity and rapid mortality achieved by Isocycloseram; and

[0021] 3. Reproductive suppression and lifecycle interruption delivered by Pyriproxyfen.

[0022] This tri-modal action provides comprehensive control across egg, larval / nymphal, and adult stages, which is not achievable using individual components or conventional binary mixtures.

[0023] A compound of Formula I chemically known as N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-( 1 , 1 -dimethylethyljthiourea, is an insecticide and acaricide effective against Aphids; Whiteflies;Spidermites; Diamondback moth; thrips; Jassids and other sucking insect-pests, etc. and prevent crops such as Cotton; Fruit trees; Ornamentals; Soybeans, etc. It belongs to Thiourea insecticide or Thiourea acaricide and have a broad spectrum, contact and stomach action with some ovicidal activity, which acts by inhibiting oxidative phosphorylation. Thus, its mode of action is an Inhibitor of mitochondrial ATP synthase.

[0024] Further, Isocycloseram is a broad-spectrum arylisoxazoline insecticide that is active against a wide range of insects such as Thrips, Caterpillars, Beetles, Flies, Mites, Diamondback moth; Cabbage white butterfly, etc for the crops such as Corn; Coffee; Cotton; Rice; Fruit including apples and pears; Brassicas, etc. Its mode of action is to act as GABA-gated chloride channel allosteric modulator and acts as a non-competitive GABA-gated chloride channel antagonist.

[0025] Moreover, Pyriproxyfen is an insecticide used to control insects such as Flies; Midges; Ants; Cockroaches; Whiteflies, etc. Also used for flea control and is effective for protecting crops such as brinjal, cotton, chilli, okra, etc. and its mode of action is to act as juvenile hormone mimic by inhibiting insect maturation process.

[0026] In view of the drawbacks associated with the prior arts, there exists a need to provide a stable synergistic insecticidal composition for synergistic and effective control of insects. Thus, the present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art. The present invention composition is synergistic, efficacious, environmentally friendly, simple to formulate, stable and is safe to use.

[0027] Thus, an insecticidal composition of the present invention aims to overcome the problems of the prior art and provides a stable synergistic insecticidal composition comprising bioactive amounts of A) a compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l-dimethylethyljthiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives.

[0028] The present invention also relates to a method for preparing stable synergistic insecticidal composition.OBJECTIVES AND ADVANTAGES OF THE INVENTION

[0029] The principal objective of the present invention is to provide a novel and effective stable synergistic insecticidal composition demonstrating high efficacy and to provide complete protection to crops, plants against insect-pests.

[0030] It is another objective of the present invention to provide improved, stable and ready to use insecticidal composition having synergistic and superior efficacious properties compared to prior art formulations or mixtures or compositions.

[0031] It is yet another objective of the present invention to provide a novel and effective stable synergistic insecticidal composition demonstrating high efficacy and high selectivity. It is yet another objective of the present invention to provide improved combination of insecticides for broad spectrum control of insects.

[0032] It is yet another objective of the present invention to provide a method and a stable synergistic insecticidal composition for controlling insect pests and insecticidal diseases.

[0033] Yet another objective of the present invention to provide a novel and effective synergistic insecticidal composition which has both preventive and curative action and is active against major life stages of major plant pathogens and that promotes plant health, growth and increases plant, crop yield in the field.

[0034] Yet another objective of the present invention is to provide a composition and method for controlling various insect-pests including but not limited to sucking insect, boring insect-pests, etc.

[0035] Another objective of the present invention is to provide one shot application of novel and effective stable synergistic insecticidal composition for killing / controlling highly resistant insects at lower application rates.

[0036] It is another objective of the present invention to provide a novel and effective stable synergistic insecticidal composition which is environmentally safe, possesses broad spectrum bio-efficacy, isnon-phytotoxic and has improved stability during the desired shelf life at various temperature and pH conditions and has enhanced efficacy and penetration capacity and is economically more beneficial.

[0037] It is another objective of the present invention to provide a novel and effective stable synergistic insecticidal composition which further comprises additives for providing the enhanced stability, enhanced permeability, improved penetration capacity, uniform spray -ability, improved rainfastness, enriched biotic factors, better flowability, enhanced spreading or coverage effect, effective storage stability, longer residual effect, improved uptake of active ingredients, and safer to the environment attributes to the composition of present invention.

[0038] Another objective of the present invention is to provide a stable synergistic insecticidal composition comprising A) a compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l-dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives. Yet another objective of the present invention is to provide a process for the preparation of a stable synergistic insecticidal composition comprising A) a compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l-dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives.

[0039] Some or all these and other objects of the invention can be achieved by way of the invention described hereinafter.

[0040] ADVANTAGES:

[0041] 1. The stable synergistic insecticidal composition of the present invention decreases the chances of resistance against insects and associated diseases and shows to have an improved stability during the desired shelf life and promotes plant health and increases plant yield in the field.2. The stable synergistic insecticidal composition of the present invention results in significant lowering the count of insect pest such as Stinkbugs, Thrips, Caterpillars, Beetles, Flies, Mites, Diamondback moth; Cabbage white butterfly, etc.

[0042] 3. The stable synergistic insecticidal composition of the present invention shows no phytotoxicity effect and provide a stable synergistic insecticidal composition that shows technical advancement over the existing combination of insecticides and also shows an improved rain fastness.

[0043] 4. The stable synergistic insecticidal composition of the present invention resulted in significantly lower incidence of insecticidal diseases in various crops such as Corn; Coffee; Cotton; Rice; Fruit including apples and pears; Brassicas, etc, as compared to the other treatments and untreated check.

[0044] SUMMARY OF THE INVENTION

[0045] Accordingly, the main aspect of the present invention is to provide a synergistic insecticidal composition having high insect-pests control efficacy.

[0046] Accordingly, the present invention relates to a synergistic insecticidal composition comprising A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives.

[0047] In another aspect, the present invention provides a stable synergistic insecticidal composition comprising:

[0048] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers esters or its derivatives; and

[0049] D) One or more agrochemically acceptable excipients.In another aspect, the present invention provides a stable synergistic insecticidal composition comprising:

[0050] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers esters or its derivatives; and

[0051] D) At least one or more additive.

[0052] In another aspect, the present invention provides a stable synergistic insecticidal composition comprising:

[0053] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives;

[0054] D) Protein-based dispersant and / or a polysaccharide -based dispersant; and

[0055] E) One or more agrochemically acceptable excipients.

[0056] In one another aspect, the present invention provides a stable synergistic insecticidal composition comprising:

[0057] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives;

[0058] D) Protein-based dispersant and / or a polysaccharide-based dispersant;

[0059] E) At least one or more additive selected from urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof; and

[0060] F) One or more agrochemically acceptable excipients.In one another aspect, the present invention provides a stable synergistic insecticidal composition comprising:

[0061] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0062] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition; and

[0063] C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives, in the range of 0.001-90% by weight of the composition.

[0064] In yet another aspect, the present invention provides a process for preparation of the stable synergistic insecticidal composition comprising:

[0065] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives.

[0066] In an aspect of the present invention, the present invention provides a stable insecticidal composition which further optionally comprises additives for providing the following attributes to the composition of present invention:

[0067] a) enhanced stability, spreading or coverage effect, and permeability,

[0068] b) improved penetration capacity and uptake of active ingredients,

[0069] c) uniform spray-ability,

[0070] d) improved rain-fastness, and enriched biotic factors,

[0071] e) better flowability and effective storage stability,

[0072] f) longer residual effect,

[0073] g) safer to the environment.

[0074] In another aspect, the present stable synergistic insecticidal composition has enhanced shelf life and effectively control wide range of insects in various crops.In another aspect, the present invention provides a stable synergistic insecticidal composition which has enhanced efficacy and penetration capacity.

[0075] DETAILED DESCRIPTION OF THE INVENTION

[0076] In describing the embodiments of the invention, specific terminology is resorted for sake of clarity. However, it is not intended that the invention be limited to specific terms so selected and it is to be understood that each specific term includes all technical equivalents that operate in a similar manner to accomplish a similar purpose.

[0077] Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods.

[0078] All technical and scientific terms used herein have the same meanings as commonly understood by someone ordinarily skilled in the art to which the present subject matter belongs.

[0079] The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure.

[0080] As used in the present disclosure, the forms "a,” "an," and "the" may be intended to include the plural forms as well, unless the context clearly suggests otherwise. Thus, for example, reference to a composition containing “a compound” includes a mixture of two or more compounds. It should also be noted that the term “or” is generally employed in its sense including “and / or” unless the content clearly dictates otherwise.

[0081] The terms "comprise / comprises", "comprising", “including”, and “having” are open ended transitional phrases and therefore specify the presence of stated features, integers, steps, operations, elements, but do not forbid the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof. The particular order of steps disclosed in the method and process of the present disclosure is not to be construed as necessarily requiring their performance as described or illustrated. It is also to be understood that additional or alternative steps may be employed.Variations or modifications to the composition of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.

[0082] The expression of various quantities in terms of “%

[0083]

[0084] or “%” means the percentage by weight, relative to the weight of the total formulation (i.e % w / w) or volume of total composition (i.e % w / v) unless otherwise specified.

[0085] The term “active ingredient” (a.i.) or “active agent” used herein refers to that component of the composition responsible for control and killing of insect-pest.

[0086] The terms “plants” and “vegetation” include, but are not limited to, germinant seeds, emerging seedlings, plants emerging from vegetative propagules, and established vegetation.

[0087] The term “crop” shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

[0088] The term “synergistic”, as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects. “Bioactive amounts” as mentioned herein means that amount which, when applied for treatment of crops, is sufficient to give effect in such treatment.

[0089] The term “control” means to inhibit the ability of insect pests to survive, grow, feed and / or reproduce, or to limit the insect pests’ related damage or loss in crop plants. To “control” insect pests may or may not mean killing the insects although, it preferably means killing the insect pests. The term “health of a plant” or “plant health” is defined as a condition of the plant and / or its products. As a result of the improved health; yield, plant vigour, quality and tolerance to abiotic or biotic stress is increased. As a result, the health of a plant is increased even in the absence of insect pressure.

[0090] Accordingly, in an especially preferred embodiment according to the invention, the health of a plant is increased both in the presence and absence of biotic or abiotic stress factors. An increase in plant vigor may for example results in an increased yield and / or tolerance to abiotic or biotic stress.One indicator for the condition of the plant is the yield. “Yield” is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits, vegetables, nuts, grains, seeds, wood (e.g., in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals). The plant products may in addition be further utilized and / or processed after harvesting.

[0091] According to embodiments of the present invention, the yield of the target plants treated according to the method of the invention, is increased synergistically.

[0092] According to embodiments of the invention, the plant vigour of the treated plant is increased. In another preferred embodiment of the invention, the plant vigour of the plants treated according to the composition of the present invention is increased synergistically.

[0093] Another indicator for the condition of the plant is the “quality” of a plant and / or its products. According to embodiments of the invention, the quality of the treated plant is increased.

[0094] In preferred embodiment of the invention, the quality of the plants treated according to the method of the invention, is increased synergistically.

[0095] The term rain fastness refers to the ability of a substance to remain effective after being applied to a surface and then exposed to rainfall or irrigation. In other words, it measures how well the substance adheres to the target surface and resists being washed away by water. A low rain fastness indicates that it may be washed off or diluted by rainfall, reducing its efficacy.

[0096] The term "Increased uptake of active ingredient" refers to the enhanced absorption or penetration of the active component of a substance into the target organism or plant. This increased uptake can lead to greater effectiveness of the product in controlling various insect including but not limited to sucking insect-pests at the same time. It may occur through various mechanisms, such as improved formulation, adjuvants that enhance penetration, or modifications to the active ingredient itself to facilitate absorption. Overall, increased uptake of the active ingredient results in improved performance of the product in achieving its intended purpose.

[0097] The term “FS” refers to a flowable concentrate formulation designed for seed treatment applications, wherein the active ingredients are suspended in an aqueous medium and are capable of forming a stable, uniform coating on seeds.The term “benefit: cost” refers to a ratio used in a cost -benefit analysis to summarize the overall relationship between the relative costs and benefits of a proposed composition. If a treatment has a benefit-cost ratio greater than 1.0, the treatment is expected to deliver a positive and better outcome.

[0098] According to embodiments of the present invention, the yield of the treated plant is increased. According to embodiments of the present invention, there is synergistic and efficacious control of various insects affecting the plants and crops.

[0099] Another embodiment of the present invention, includes within its scope, all possible salts, esters and derivatives of the active ingredients of the composition.

[0100] It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification.

[0101] Unless indicated otherwise, the technical and scientific terms employed herein shall be construed in accordance with their conventional meaning as understood by a person skilled in the relevant art. In the event of any inconsistency, the definitions and explanations set forth in the present specification shall prevail.

[0102] The composition or formulation according to the present invention has a reduced or zero phytotoxicity with respect to agricultural crops. This results in the present invention effective and possible in the selective control of insects.

[0103] In an embodiment, the stable synergistic pesticidal composition or formulation has a reduced or zero phyto toxicity with respect to important agricultural crops. This results in the present invention effective and possible in the selective control of insect including but not limited to sucking insectpest.It has been observed by the inventor of the present invention that the composition of present invention not only provides synergistic effect but also provides anti-aging effect to the crops or plants to which it is applied. In addition, the stable synergistic insecticidal composition of the present invention is effective against major developmental stages of insect pests including egg, nymph, larva, pupa, and adult.

[0104] In an embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0105] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyljthiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocy closeram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives.

[0106] In another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0107] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyljthiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocy closeram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives; and

[0108] D) One or more agrochemically acceptable excipients.

[0109] In another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0110] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyljthiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocy closeram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives; and

[0111] D) At least one or more additive.

[0112] In another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocy closeram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives;

[0113] D) Protein-based dispersant and / or a polysaccharide -based dispersant; and

[0114] E) One or more agrochemically acceptable excipients.

[0115] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0116] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocy closeram or its agrochemically acceptable salts, esters, isomers and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives;

[0117] D) Protein-based dispersant and / or a polysaccharide -based dispersant;

[0118] E) At least an additive selected from urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof; and

[0119] F) One or more agrochemically acceptable excipients.

[0120] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0121] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0122] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition; and

[0123] C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-90% by weight of the composition.In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0124] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0125] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition;

[0126] C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-90% by weight of the composition; and

[0127] D) One or more agrochemically acceptable excipients.

[0128] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0129] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0130] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition;

[0131] C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-90% by weight of the composition; and

[0132] D) At least one or more additive.

[0133] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0134] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0135] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition; andC) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-90% by weight of the composition;

[0136] D) Protein-based dispersant and / or a polysaccharide -based dispersant, in the range of 0.001- 50% by weight of the composition; and

[0137] E) One or more agrochemically acceptable excipients.

[0138] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0139] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0140] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition; and

[0141] C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-90% by weight of the composition;

[0142] D) Protein-based dispersant and / or a polysaccharide -based dispersant, in the range of 0.001- 50% by weight of the composition; and

[0143] E) At least one or more additive.

[0144] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0145] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-90% by weight of the composition;

[0146] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-90% by weight of the composition; and

[0147] C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-90% by weight of the composition;D) Protein-based dispersant and / or a polysaccharide -based dispersant, in the range of 0.001- 50% by weight of the composition;

[0148] E) At least one or more additive selected from urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof, in the range of 0.001-20% by weight of the composition; and

[0149] F) One or more agrochemically acceptable excipients.

[0150] In one another embodiment, the present invention provides a stable synergistic insecticidal composition comprising:

[0151] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, in the range of 0.001-40% by weight of the composition;

[0152] B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives, in the range of 0.001-80% by weight of the composition; and

[0153] C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.001-50% by weight of the composition.

[0154] In yet another embodiment, the present invention provides a process for preparation of the stable synergistic insecticidal composition comprising:

[0155] A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters or its derivatives.

[0156] In accordance with an embodiment of the present invention, there is provided a synergistic insecticidal composition comprising active ingredients present in the weight % range (w / w% or w / v%) as given below:

[0157]

[0158]

[0159] According to embodiments of the present invention, the stable synergistic insecticidal composition results in an increased yield of the treated plant. Accordingly, there is synergistic and efficacious control of various insect-pests and affecting the plants and crops. The present invention includes within its scope, all possible salts, esters and derivatives of the active ingredients of the composition.

[0160] According to embodiments of the present invention, the stable synergistic insecticidal composition or formulation according to the present invention has a reduced or zero phytotoxicity with respect to important agricultural crops. This results in the present invention effective and possible in the selective control of insect-pests and associated diseases.

[0161] In an embodiment of the present invention, the stable synergistic insecticidal composition further optionally comprises additives, for providing the following attributes to the composition of present invention:

[0162] a) enhanced stability, spreading or coverage effect, and permeability,

[0163] b) improved penetration capacity and uptake of active ingredients,

[0164] c) uniform spray-ability,

[0165] d) improved rain-fastness, and enriched biotic factors,

[0166] e) better flowability and effective storage stability,

[0167] f) longer residual effect, andg) safer to the environment.

[0168] In another embodiment of the present invention, the stable synergistic insecticidal composition of the present invention further comprises one or more agrochemically acceptable excipients including but not limited to the group comprising surfactant(s), dispersing agent(s), co -dispersant, anti-freezing agent(s), emulsifier(s) (anionic and non-ionic), anti-foaming agent / defoamer(s), wetting agent(s), antimicrobial / anti-bacterial agent(s), anti-caking agent , thickening agent / thickener(s), quick coating agent(s) or sticking agents / sticker(s), spreader(s), binder(s), pH-adjusting agent(s), anti-caking agent(s), adjuvant(s), filler(s) / suspension aid(s), colorant(s), carrier(s), buffering agent(s), polymer(s)rheology modifier(s), solvent(s), co-solvent(s) , biocide or a combination thereof. Additional components may also be included, e.g., protective colloids, carriers, adhesives, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active materials can be combined with any solid or liquid carrier / additive, which complies with usual formulation techniques.

[0169] An adjuvant used in the present invention is any material that is added to an insecticidal formulation to enhance or modify the performance of the formulation. Adjuvants used in the present invention to make it a safer to ecological environmental, having low toxicity and having no phytotoxicity effects on any part of the plant.

[0170] Examples of surfactant(s) and / or dispersing agent and / or co-dispersant used herein include but not limited to the group comprising polymeric anionic dispersant, polyarylphenyl ether phosphate, tristyryl phenol ethoxylate, Poly methyl methacrylate -polyethylene glycol graft copolymer, acrylate copolymer, acrylic copolymer, Ammonium Distyrylphenyl Ether Sulphate, Alkyl polyglucoside, Sodium lauryl sulphonate, polymeric dispersant, Calcium salt of alkyl benzene sulphonate, Calcium doceylbenzene sulphonate, Non-ionic surfactant, polyalkoxylated butyl ether, acrylic graft copolymer, Sodium methyl oleoyl taurate , non-ionic proprietary surfactant, Methyl naphthalene sulfonic acid, polymer with formaldehyde, sodium salt, ethoxylated tristryl phenol Sulphate, alkyl naphthalene formaldehyde condensate, sodium alkyl naphthalene sulfonate blend, naphthalene sulfonic acid, phenol sulfonic acid-formaldehyde polycondensation as sodium salt, sodium salt condensate with formaldehyde, ethoxylated oleyl cetyl alcohol, Acrylate graft Copolymer, Anionic tristyrylphenol phosphate surfactant, Sodium Alkyl Naphthalene SulfonateCondensate, polyalkelene glycol ether, Sodium Isopropyl Naphthalene Sulfonate, Dialkyl naphthalene sulphonate sodium salt, Naphthalene Sulphonic Acid, Formaldehyde, Ethoxylated fatty glyceride , Ethoxylated Polyarylphenol Phosphate Ester, Lignosulfonic Acid, Sodium Salt, Sulfomethylated, ethoxylated fatty alcohol, methyl naphthalene sulfonic acid, polymer with formaldehyde sodium salt, Sodium polymethyl methacrylate, sodium polyacrylate, alkyl naphthalene sulfonate formaldehyde condensate or its sodium salt , Sodium Naphthalene Sulfonate formaldehyde condensate, Amine Salt of a Phosphated Tristyryl Phenol Ethoxylate, sodium lignosulphonate, calcium lignosulphonate, Sodium Alkyl Naphthalene Sulfonate, alkylphenol polyoxyethylene ether, sodium salt of naphthalene sulfonate condensate, alkyl naphthalene sulfonate condensate blend, polymeric surfactant, sodium salt of alkyl naphthalene sulfonate, sodium salt of methyl naphthalene sulfonate, lignosulphonate, polycarboxylate or its sodium salt, naphthalene sulfonic acid-branched and linear derivative sodium salts, fatty alcohol polyoxyethylene ether, alcohol polyglycol ether, non-aqueous dispersant - non-ionic polymeric surfactant, aqueous dispersant - polymeric amphoteric aqueous dispersant or mixtures thereof and present in the range of 0.01-20% by weight of the composition.

[0171] Examples of wetting agent used herein include but not limited to the group comprising tristyrylphenol ethoxylate non-ionic emulsifier, mixture of non-ionic surfactants, Blend of Anionic and Non-ionic, Sodium dioctyl sulfosuccinate, alkoxylated alcohol, block copolymer, alcohol polyglycol ether, Poly alkyleneoxide modified poly dimethylsiloxane, alkyl olefin sulphates, dioctyl sulpho succinate, alkyl naphthalene sulphate , Di-isopropyl Napthalene sulphonate, Benzenesulfonic acid, Hydroxy polymer with formaldehyde, Phenol and Urea sodium salt, Polyalkoxy ethers, alkyl phenol ethoxylates, tri-styrenated phenol ethoxylate, EO / PO block copolymers, butyl polyalkylene oxide block copolymer, sodium lauryl sulphate, sodium sulphates or mixtures thereof and present in the range of 0.001-20% by weight of the total composition.

[0172] Examples of defoamer or antifoaming agent used herein include but not limited to the group comprising silicon emulsion based anti-foam agents, Siloxane polyalkyleneoxide, Polydimethylsiloxane, trisiloxane ethoxylates, silicone oil, silicone compound, C10~C20 saturated fat acid compounds or C8~C10 aliphatic alcohols compound, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide polydimethylsiloxane emulsion and mixtures thereof and present in the range of 0.001-5.0 % by weight of the composition.Examples of anti-freezing agent used herein include but not limited to the group comprising glycol, propylene glycol, mono ethylene glycol, glycerine, diethylene glycol, sorbitol, urea, mannitol, Aluminum Magnesium Silicate, polyethylene glycol or mixtures thereof and present in the range of 0.01-20% by weight of the composition.

[0173] Examples of filler used herein include but not limited to the group comprising silicon dioxide, China-clay, kaolin, starch, bentonite, precipitated silica, fumed silica, lactose monohydrate, talcum powder, lactose, ammonium sulphate, water, sodium Sulphate, magnesium sulphate, sodium citrate, sodium chloride, potassium chloride and mixtures thereof and present in the range of 0.001-60 % by weight of the composition.

[0174] Examples of anti-caking agent used herein include but not limited to the group comprising of talc, hydrous magnesium silicate, magnesium carbonate, calcium carbonate, precipitate silica, silica powder, hydrophobic fumed silica and mixtures thereof and present in the range of 0.001-10% by weight of the composition.

[0175] Examples of carrier used herein include but not limited to the group comprising of silicon dioxide, china clay, kaolin, starch, bentonite, precipitated silica, silica powder, fumed silica, lactose monohydrate, talcum powder, lactose, ammonium sulpha or mixture thereof and present in the range of 0.1-45 % by weight of the composition.

[0176] Examples of biocide or anti-microbial / anti-bacterial agent(s), agent(s) used herein include but not limited to the group comprising of 1, 2,- Benzisothiazolin -3-one, blends of 1,2-benzisothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, blends of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, zinc 1 -hydroxy -2(1H)-pyridinethione, blends of zinc l-hydroxy-2(lH)-pyridinethione and zinc oxide, and 2-n-octyl-4-isothiazolin-3-one or mixture thereof and present in the range of 0.001 to 10 % by weight of the composition.

[0177] Examples of the rheology modifier or thickeners or thickening agents or suspending agent(s)used herein include but not limited to the group comprising of polysaccharides, carboxymethyl cellulose, bentonite clay, aluminium magnesium silicate, hydroxy propyl cellulose, Sodium methylcellulose, xanthan gum, gum arabic, Silica powder, gaur gum, carbomer, chitosan, precipitated silica or mixtures thereof and present in the range of 0.001-5.0 % by weight of the composition. Examples of emulsifier(s) used herein include but not limited to the group comprising of araliphatic or aliphatic non-ionic oil emulsifier-ethoxylated sorbitol hexaester derived from oleic acid, emulsifier such as ethoxylated mono-, di- and trialkylphenols, ethoxylates of long-chain alcohols and polyethylene oxide / polypropylene oxide homo- and copolymers, Anionic and nonionic blend of castor oil ethoxylate, Hydrophobically Modified Polyacrylate Copolymer, Polymeric emulsifier (Nonionic polyalkylene oxide block copolymer), Ethoxylates of long-chain alkanols, Castor oil ethoxylate , ethoxylated monoalkylphenol, ethoxilates of cetyl alcohol and / or stearyl alcohol; water soluble emulsifier, Emulsifier (Blend of Anionic and Non-ionic), Tristyrylphenol ethoxylate-20 mole, Alkyl diphenyl sulfonates, Ethoxylated Tristyrylphenol ,and Anionic emulsifier such as alkali metal and ammonium salts of alkyl sulfates, of hemisulfates of ethoxylated alkanols and ethoxylated alkylphenols, alkylsulfonic acids, bis(phenylsulfonic acid) ethers and their alkali metal or ammonium salts, sodiumlaurylsulfate, sodium (cetyl / stearyl) sulfate or mixture thereof and present in the range of 0.001-20 % by weight of the composition.

[0178] Examples of emulsifier(s) used herein is / are selected from group or non-ionic, ionic, or a blend or combination thereof, present in the range of 0.001-20 % by weight of the composition.

[0179] Examples of Non-ionic emulsifier(s) used herein is / are selected from group comprising of Araliphatic or aliphatic non-ionic oil emulsifier - ethoxylated sorbitol hexa-ester derived from oleic acid, Ethoxylated mono-, di- and trialkylphenols, Ethoxylates of long-chain alcohols, Polyethylene oxide / polypropylene oxide homo- and copolymers, Hydrophobically Modified Polyacrylate Copolymer (nonionic polymeric dispersant), Polymeric emulsifier (Nonionic polyalkylene oxide block copolymer), Ethoxylates of long-chain alkanols, Castor oil ethoxylate, Ethoxylated monoalkylphenol, Ethoxylates of cetyl alcohol and / or stearyl alcohol, Tristyrylphenol ethoxylate, Ethoxylated Tristyrylphenol, Non-ionic water-soluble emulsifiers, or a combination thereof.

[0180] Examples of the ionic emulsifier(s) used herein is / are selected from group comprising of alkali metal and ammonium salts of alkyl sulfates, hemisulfates of ethoxylated alkanols, hemisulfates of ethoxylated alkylphenols, alkylsulfonic acids, bis(phenylsulfonic acid) ethers and their alkalimetal or ammonium salts, sodium lauryl sulfate, sodium (cetyl / stearyl) sulfate, Ionic water-soluble emulsifiers or combination thereof.

[0181] Examples of solvent(s) and co-solvent(s) used herein include but not limited to the compounds selected from polar and non-polar solvent from the group comprising heavy aromatic hydrocarbon, diethyl phthalate, N-Methyl-2 -pyrrolidone (NMP) , Solvent Naphtha (Solvent C-9), Di methyl sulfoxide (DMSO), Dimethylformamide (DMF), Dialkylene glycol dialkyl ethers, Methyl soyate oil, N- alcohol, 2-ethyl hexanol , alkyl amide, vegetable oil, mineral oil, distilled water, aromatic solvents, water or mixtures thereof and present in the range of 0.01 to 20% by weight of the composition.

[0182] Examples of colouring pigment(s) / dye(s) used herein include but not limited to the red dye FGR03, Permanent Rubin L5-B-01, pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1 , pigment blue 80, pigment yellow 1 , pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48: 1 , pigment red 57: 1 , pigment red 53:1 , pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, violet 23, acid red 51 , acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108 or mixtures thereof and present in the range of 0.001- 10.0 % by weight of the composition.

[0183] Examples of polymer(s) used herein include but not limited to the isocyanate, polyisocyanate used is toluene -2, 4-diisocyanate (TDI), diethylenetriamine (DETA), ethylene diamine (EDA), triethylenetetramine (TETA), and / or hexamethylene diamine (HMDA), diethanolglycine sodium salt, ethanoldiglycine disodium salt, hydroxymonocarboxylic acid compounds, hydroxydicarboxylic acid compounds, tetrasodium N,N-bis(carboxylatomethyl)-L-glutamate (GLDA), methylene diisocyanate (MDI), and / or low molecular weight polydiisocynates or mixtures thereof and present in the range of 0.01- 5.0 % by weight of the composition.

[0184] Examples of binder(s) used herein include but not limited to the group of compounds selected from polyvinylpyrrolidone (PVP), polyvinyl alcohol, methyl cellulose, and carboxymethyl cellulose, sodium starch glycolate, polyisocyanate, polyterpene resin or mixtures thereof and present in the range of 0.01- 40 % by weight of the composition.Examples of the preservative(s) used herein include but not limited to the group of compounds selected from Methyl paraben (Methyl p-hydroxybenzoate), Propyl paraben (Propyl p-hydroxybenzoate), Methylisothiazolinone (MIT), Chloromethylisothiazolinone (CMIT), Benzisothiazolinone (BIT), DMDM Hydantoin, Imidazolidinyl Urea, Phenol, Cresol, Chlorocresol, Benzoic acid, Sorbic acid, Salicylic acid, Thymol (natural preservative), Sodium benzoate, Potassium sorbate, Bronopol (2-bromo-2-nitropropane-l,3-diol), Propyl para-hydroxy -benzoate or mixture thereof and present in the range of 0.01- 35 % by weight of the composition. Examples of stabilizer(s) used herein include but not limited to the compounds selected from butylated copolymer, Butylated Hydroxy Toluene (BHT), Butylated Hydroxyanisole (BHA), Ascorbic Acid (Vitamin C), Tocopherol (Vitamin E), Benzophenone-3 (a benzotriazole UV absorber), Hindered Amine Light Stabilizers (HALS), Propylene Glycol, Oxanilide derivatives, Ethoxylated Alkylphenols (e.g., nonylphenol ethoxylates), Block copolymers, Polyvinyl alcohol (PVA), Acrylic or Butylated Copolymers, Citric acid / Sodium citrate, Phosphates (e.g., Sodium or Potassium phosphate), Acetates (e.g., Sodium acetate), EDTA (Ethylenediaminetetraacetic acid), Citric acid (dual role: pH and chelation), NTA (Nitrilotriacetic acid), Xanthan gum, Attapulgite clay, Bentonite, Hydroxypropyl methylcellulose (HPMC) or mixture and present in the range of 0.01- 35 % by weight of the composition.

[0185] Examples of the pH adjusting agent(s) / buff ering agent(s) used herein include but not limited to the compounds selected from Citric Acid, Citric Acid Monohydrate, Citric Acid Dihydrate, Sodium Citrate, Potassium Citrate, Tartaric Acid, Potassium Hydrogen Tartrate, Lactic Acid, Sodium Phosphate (Monobasic or Dibasic), Potassium Phosphate (Monobasic / Dibasic), Sodium Bicarbonate, Sodium Carbonate, Ammonium Acetate / Ammonium Hydroxide, Acetic Acid, Sodium Acetate, Borax (Sodium Borate) or mixture thereof and present in the range of 0.01- 25 % by weight of the composition.

[0186] In yet another embodiment of the present invention, the present stable synergistic insecticidal composition can be formulated as one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscibleflowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo -emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) or a mixed formulation of CS and EW (ZW). In yet another embodiment of the present invention, the invention further provides the process for preparation of the said insecticidal composition, wherein the said composition can be one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oildispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) or a mixed formulation of CS and EW (ZW).

[0187] In further embodiment of the present invention, depending on the formulation type, they comprise one or more liquid or solid carriers, appropriate surfactants (such as dispersants, protective colloids, emulsifiers, wetting agents and tackifiers), and appropriate further excipients which are customary for formulating insecticidal composition. Further excipients include e.g., organic and inorganic thickeners, bactericides, antifreeze agents, antifoams, colorants and, for seed formulations, adhesives.

[0188] In one of the embodiments of the present invention, suitable carriers include liquid and solid carriers. Liquid carriers include e.g. non-aqueous solvents such as cyclic and aromatichydrocarbons, e.g. paraffins, tetrahydronaphthalene, alkylated naphthalenes and their derivatives, alkylated benzenes and their derivatives, alcohols such as methanol, ethanol, propanol, butanol and cyclohexanol, ketones such as cyclohexanone, strongly polar solvents, e.g. amines such as N-methylpyrrolidone, and water as well as mixtures thereof. Solid carriers include e.g. mineral earths such as silicas, silica gels, silicates, talc, kaolin, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide, ground synthetic materials, fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, ureas, and products of vegetable origin such as cereal meal, tree bark meal, wood meal and nutshell meal, cellulose powders, or other solid carriers.

[0189] In one embodiment, additives such as Siloxane Polyalkyleneoxide Copolymer or an organosilicone based powder adjuvant that can enhance the water absorption efficiency of hard-to-wet soils by increasing their water infiltration, drainage, water retention and hydrophilicity; tallow amine ethoxylate increases the biocompatibility in environmental applications and help in the interactions of active ingredients; ammonium sulphate help is resistant to leaching and helps the crop to utilize nitrogen efficiently; urea clathrate provides superior wetting and spray retention properties and serves as an excellent nitrogen source for crops and epoxide soybean oil provides increased active ingredient uptake and improved rain fastness to the composition; and epoxide soybean oil provides increased active ingredient uptake and improved rain fastness to the composition.

[0190] In one embodiment of the present invention, Siloxane Polyalkyleneoxide Copolymer has superspreading ability, it significantly lowers the surface tension of spray solutions, allowing them to spread uniformly across plant surfaces, including waxy or hard-to-wet leaves. This enhances the penetration and absorption of pesticides, leading to improved efficacy. Siloxane Polyalkyleneoxide Copolymer is especially effective in low-volume spray applications and helps increase rainfastness and reduce runoff. Its use supports more efficient pesticide delivery, often allowing for reduced application rates.

[0191] In one embodiment of the present invention, the inclusion of both Siloxane Polyalkyleneoxide Copolymer super-wetter and tallow amine ethoxylate in a formulation significantly enhances its surface activity and delivery efficiency. Siloxane Polyalkyleneoxide Copolymer, a trisiloxane-based super-spreader, dramatically reduces surface tension, enabling rapid and uniform spreading of the solution across plant surfaces, even on waxy or water-repellent leaves. In synergy, tallowamine ethoxylate, a non-ionic surfactant, acts as a powerful wetting and penetration agent, improving the absorption of active ingredients into plant tissues. This combination ensures maximum bioavailability and performance of insecticidal actives.

[0192] In one embodiment of the present invention, ammonium sulphate particularly as a water conditioner and adjuvant. It helps improve the efficacy of pesticides, by binding with hard water cations such as calcium and magnesium that would otherwise reduce pesticide absorption and activity. By preventing these ions from interacting with the active ingredient, ammonium sulphate enhances uptake into plant tissues. It may also act as a nitrogen source, supporting plant growth. In one embodiment of the present invention, Epoxide soybean oil, a bio-based plasticizer and solvent, improves formulation compatibility, reduces phytotoxicity, and enhances the dispersion of active compounds. Derived from renewable sources, both additives support sustainable agriculture by offering low toxicity, biodegradability, and improved formulation efficiency without compromising efficacy.

[0193] In one embodiment of the present invention, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil are additives used individually or in combination to enhance the performance of pesticidal formulations. Siloxane Polyalkyleneoxide Copolymer, a superspreading organosilicone surfactant, reduces surface tension and improves leaf coverage and penetration of active ingredients. Tallow amine ethoxylates, as non-ionic surfactants, aid in emulsification and enhance pesticide uptake. Ammonium sulphate serves as a water conditioner, preventing hard water ions from reducing pesticide efficacy, while also acting as a nitrogen source. Epoxide soybean oil, a biodegradable solvent and plasticizer, improves formulation stability, compatibility, and reduces phytotoxicity. Used alone or synergistically, these additives optimize spray performance, improve bioavailability of actives, and support sustainable and effective crop protection.

[0194] In another embodiment of the present invention, additives are incorporated to enhance the overall performance of the insecticidal composition. These additives contribute to improved stability, ensuring the formulation remains effective over time. They also enhance the flowability of the composition, making it easier to apply. Furthermore, these additives promote better uptake of active ingredients, improve soil water retention, and increase plant resilience to stress, ultimately boosting the overall effectiveness of the insecticidal composition.The said additives are stable and hygroscopic resulting in an efficient uptake and functioning. In one embodiment, additives such as urea clathrate, Siloxane Polyalkyleneoxide Copolymer or organosilicone -based powder adjuvant, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof, present in the range of 0.001-20% by weight of the composition.

[0195] In one embodiment, Protein-based dispersant is selected from collagen, casein, albumin, polyamino acids, hemoglobin, gelatin, gelatin derivatives, and combinations thereof, present in the range of 0.001 - 50% by weight of the composition.

[0196] In one embodiment, Polysaccharide -based dispersant is selected from gum Arabic, carrageenan, gellan gum, starch, microcrystalline cellulose, sodium carboxymethyl cellulose, microfibrillated cellulose, combination of microcrystalline cellulose and sodium carboxy methyl cellulose, carboxymethyl cellulose, methyl cellulose, ethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, and combinations thereof, present in the range of 0.001 - 50% by weight of the composition.

[0197] In one embodiment, the inclusion of protein-based and / or polysaccharide -based dispersants in the present insecticidal composition results in an unexpected and remarkable synergistic effect. This unique combination significantly enhances the suspensibility, stability, and dispersibility of the formulation. These properties collectively contribute to an outstanding field efficacy, ensuring the composition performs effectively under real-world agricultural conditions. The synergistic interaction between these dispersants not only improves the overall performance of the insecticidal composition but also provides enhanced stability over extended periods, thereby ensuring prolonged efficacy in the field. Further, enhanced dispersibility also facilitates uniform distribution, ensuring that the active ingredients are effectively delivered to the target pests.

[0198] In one embodiment, additives such as Siloxane Polyalkyleneoxide Copolymer or an organosilicone based powder adjuvant that can enhance the water absorption efficiency of hard-to-wet soils by increasing their water infiltration, drainage, water retention and hydrophilicity; tallow amine ethoxylate increases the biocompatibility in environmental applications and help in the interactions of active ingredients; ammonium sulphate help is resistant to leaching and helpsthe crop to utilize nitrogen efficiently; urea clathrate provides superior wetting and spray retention properties and serves as an excellent nitrogen source for crops and epoxide soybean oil provides increased active ingredient uptake and improved rain fastness to the composition; and epoxide soybean oil provides increased active ingredient uptake and improved rain fastness to the composition. The said additives are stable and hygroscopic resulting in an efficient uptake and functioning.

[0199] In one embodiment, additives such as urea clathrate, Siloxane Polyalkyleneoxide Copolymer or Siloxane Polyalkyleneoxide Copolymer super-wetter, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof, present in the range of 0.001 - 20% by weight of the composition.

[0200] In yet another embodiment of the present invention, the present stable synergistic insecticidal composition can be applied to various crops and plants including but not limited to Cotton, Potato, Soyabean, Cabbage, Chilli, Brinjal, Cardamom, Citrus, Watermelon, Okra, Tomato, Red-gram, Groundnut, Soybean, Rice, Maize, Cor, Coffee, Pears, Brassicas, etc.

[0201] In yet another embodiment of the present invention, the present insecticidal composition provides synergistic and effective control against insects including but limited to Whiteflies, Aphids, Thrips, Jassids, Mites, Diamond back moth, Capsule borer, Red spider mites, Whitefly (Bemisia tabaci), Thrips (Thrips tabaci), Jassid (Amrasca biguttida biguttula), Aphid Aphis gossypi), Whitefly Bemisia tabaci), Thrips {Thrips tabaci) and Mites {Polyphagotarsone mus latus), Shoot and Fruit Borer, Leaf Feeder, DBM, Yellow Mites, Thrips and Fruit borer, Ball worm, Gram Pod Borer and Spotted Pod borer, thrips and Jassids, Leaf worm, semi-looper, Gridle Beetle, Stem fly, Fall Armyworm, Stem Borer, etc.

[0202] In yet another embodiment, the composition according to the invention can be applied to any and major developmental stages of insect pests, such as egg, nymph, larva, pupa, and adult. The insect pests may be controlled by contacting the target insect pest, habitat, breeding ground or its locus with a bioactive amount of the novel and inventive composition of the present invention. In yet another embodiment, the present stable synergistic insecticidal composition of the present invention can be applied to a plant / crop / soil by spraying, rubbing, atomizing, irrigating,evaporating, dusting, fogging, broadcasting, pouring, mist blowing, soil mixing, foaming, painting, spreading-on, drenching, dipping or drip irrigation, etc.

[0203] In yet another embodiment, the composition of the present invention can also comprise or may be applied together and / or sequentially with further active compounds. These further compounds can be selected from fertilizers or micronutrient donors or microorganisms or other preparations that influence plant growth, such as inoculants (e.g., a strain of nitrogen-fixing bacteria), and plant inducers.

[0204] Through the insecticidal composition of present invention, the number of applications to control wide range of insects appearing at the same time is minimised. The composition of present invention is highly safe to the user and to the environment. The composition offers the user a single homogenous application eliminating the need for tank mix. The composition also is cost-effective as it provides much greater simultaneous control and can be used in a variety of crops with a broader spectrum of protection.

[0205] Plant disease control is ordinarily accomplished by applying an effective amount of a synergistic insecticidal composition of the present invention either pre -or post-infection, to the portion of the plant to be protected such as the roots, stems, foliage, fruit, seeds, tubers or bulbs, or to the media (soil or sand) in which the plants to be protected are growing. The composition can also be applied to the seed to protect the seed and seedling.

[0206] Thus, before describing the present invention in detail, it is to be understood that this invention is not limited to particularly exemplified systems or process parameters that may of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control.In one embodiment, the composition according to the present invention acts synergistically to control pests in various crops.

[0207] Representative examples of the present composition of this invention are provided below and are not to be construed as limiting in any manner:

[0208] EXAMPLES: GENERAL RECIPE OF COMPOSITION OF PRESENT INVENTION:

[0209]

[0210]

[0211]

[0212]

[0213]

[0214]

[0215] Example A: Isocycloseram 24 % + Pyriproxyfen 1.9% + Compound of Formula 115 % ZC

[0216]

[0217]

[0218] FORMULATION PROCESS:

[0219] A. Preparation of Pyriproxyfen capsule suspension

[0220] Preparation of organic slurry - The Solvent C-9 was charged to the reactor and Pyriproxyfen was added and mixed thoroughly. When it was completely dissolved, the agitator was started for stirring the mass. Isocyanate was then charged into the reactor and mixed thoroughly for 15 minutes. The whole mass was heated and the temperature was adjusted to 55-60 °C.

[0221] Preparation of aqueous slurry - Water was charged into the aqueous phase reactor and agitation was initiated, while a portion of the water was reserved for subsequent adjustment of active ingredient content (volume make-up). Acrylate copolymer and Poly dimethylsiloxane emulsion were added to the reactor and agitated to ensure complete blending, while avoiding dust formation. The whole mass was heated and the temperature was adjusted to 55-60 °C. Then, 1,2-benzisothiazolin-3-one was added.Stirring and circulation were kept on, and the whole mass was circulated for 30 minutes through a homogenizer.

[0222] Addition of organic phase into aqueous phase - The organic phase was charged into the aqueous phase and mixed well. After final mixing, Diethylenetriamine was added and the temperature was maintained at 55-60 °C for 2 hours

[0223] B. Preparation of SC Formulation

[0224] The mixture of surfactants (Lignosulfonate, acrylic graft copolymer, Polymeric dispersant), Polydimethylsiloxane emulsion, and Propylene Glycol (PG) was first diluted in the required demineralized water and solubilized by high shear mixing. Isocycloseram Technical and Compound of Formula I Technical were then added and mixed to form a homogeneous mass. The mixed mass was ground in a bead mill until a mean particle size of below 5 microns was obtained.

[0225] Preparation of 2% gum solution - 100 g demineralized water was taken, 0.2 g of 1,2-benzisothiazolin-3-one was added, and then 2 g xanthan gum was slowly added under stirring. After grinding, the 2% water solution of xanthan gum was added under low stirring. The mixture was stirred until homogeneous and quality parameters were checked.

[0226] C. Mixing of Formulation for ZC

[0227] Part A (CS formulation) was mixed into Part B (SC formulation) in another reactor and mixed well for 1 hour to form the ZC formulation.

[0228] STABILITY DATA: Isocycloseram 24 % + Pyriproxyfen 1.9% + Compound of Formula I 15 % ZC

[0229]

[0230]

[0231] EXAMPLE B: Isocycloseram 2.1 % + Pyriproxyfen 22% + Compound of Formula 13 % SE

[0232]

[0233]

[0234] MANUFACTURING PROCESS

[0235] I. Preparation of EW

[0236] Benzyl alcohol and Pyriproxyfen technical were mixed to prepare the organic phase. In another vessel, demineralized water and Calcium dodecylbenzene sulfonate were mixed to prepare the aqueous phase. The two phases were then mixed to obtain the EW part.

[0237] II. Preparation of SC

[0238] DM water, l,2-Benzisothiazolin-3-one, Polymeric dispersant (non-acrylic type), Polydimethylsiloxane, Propylene Glycol, tristyryl phenol ethoxylate, Isocycloseram technical and Compound of Formula I were mixed. This slurry was milled to achieve a particle size of D90 less than 10 microns.

[0239] III. Preparation of SE

[0240] The two parts, i.e., the EW and SC, were mixed together. Xanthan gum was then added and mixed well to obtain the SE formulation.

[0241] STABILITY DATA: Isocycloseram 2.1 % + Pyriproxyfen 22% + Compound of Formula I 3 % SE

[0242]

[0243]

[0244] Example C: Isocycloseram 7.2 % + Pyriproxyfen 8.4% + Compound of Formula 124 % SC

[0245]

[0246]

[0247] Manufacturing Process: SC Formulation

[0248] Step 1: Preparation of 2% Gum Solution: Charge Xanthan gum ,l,2-benzisothiazoline-3-one into water as per composition and homogenize. It should be made 12-18 hour prior to use.

[0249] Step 2: Charge DM water and 1 ,2-propylene glycol into designated vessel and mix thoroughly.

[0250] Step 3: Now add Dispersing agent, co-dispersing agent, wetting agent, and suspending agent into the vessel having water and homogenise the contents for 45 - 60 minutes using high shear homogeniser.

[0251] Step 4: Then add all required technical as per composition into this premix slowly and homogenised to get uniform slurry ready for grinding.

[0252] Step 5: Before grinding half the quantity of antifoam was added and then material was subjected to grinding in Dyno mill till desired particle size is achieved.

[0253] Step 6: Remaining antifoaming agent was added after completing grinding process and before sampling for in process analysis.Step 7: Finally, 2% Xanthan gum solution was added to this formulation and homogenized for 30 minutes.

[0254] Step 8: Obtained final formulation was checked for quality check.

[0255] STABILITY DATA: Isocycloseram 7.2 % + Pyriproxyfen 8.4% + Compound of Formula I 24 % SC

[0256]

[0257] Evaluation of synergistic effect of stable synergistic pesticidal composition of the present inventionA synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2).

[0258] The synergistic action expected for a given combination of two active components can be calculated as follows:

[0259] XY

[0260] E = (X + Y) - - 100

[0261] The synergistic action expected for a given combination of three active components can be calculated as follows:

[0262] (XY+YZ+XZ) (XYZ)

[0263] E = (X + Y + Z) - + - 100 10000

[0264] Where:

[0265] E represents expected percentage of pesticidal control for the combination of the two or three active ingredients at defined doses (for example equal to x, y and z, respectively).

[0266] X is the percentage of pesticidal control observed by the compound (Isocycloseram) at a defined dose (equal to x).

[0267] Y is the percentage of pesticidal control observed by the compound (Pyriproxyfen) at a defined dose (equal to y).

[0268] Z is the percentage of pesticidal control observed by the compound (Compound of Formula I) at a defined dose (equal to z).

[0269] When the percentage of pesticidal control observed for the combination is greater than the expected percentage, there is a synergism effect.

[0270] Observed control (%)

[0271] Ratio = - Expected control (%)

[0272] Ratio of O / E > 1, synergism observed

[0273] The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.Experiment for Synergistic activity of stable synergistic insecticidal composition of the present invention:

[0274] Example 1- Details of experiment on brinjal crop:

[0275] Field experiment for synergistic activity of Isocy closeram + Pyriproxyfen + Compound of Formula I for the control of whitefly and shoot and fruit borer in brinjal crop was conducted.

[0276] To evaluate the synergistic effect, brinjal [Variety: Pusa Hybrid 5] crop was sown in 5 m x 5 m plots maintaining 75 cm x 45 cm plant distance with three replications and in Randomized Block Design (RBD). The treatments were applied as foliar spray in the form of SC formulation @ 625 ml / ha. Each active component was applied twice in different ternary and binary combinations. Solo components were also taken for comparison. The treatment details are as under:

[0277]

[0278]

[0279] The treatments T1 to T28 were used as foliar application whereas no treatment was done for T29 control treatment. Two applications of each treatment were carried out at 15 -days intervals, initiated upon reaching the Economic Threshold Level (ETL) of the target insect pests.

[0280] Whitefly (Bemisia tahaci) incidence is recorded by counting the number of adult whiteflies on the top, middle, and bottom leaves of five randomly selected plants per plot, preferably during early morning hours, and the average population per leaf is calculated before and after treatment. Shoot and fruit borer {Leucinodes orhonalis) infestation is observed by counting the total number of shoots and fruits and the number of damaged shoots and fruits from the same selected plants at each observation. Based on the percent reduction at 14 days after second spray in insect population, expected control was calculated and compared with observed control. The results of the trial have been presented here under in Table 1A.

[0281] Table 1A: Synergistic effect of stable synergistic insecticidal composition of the present invention comprising Isocycloseram + Pyriproxyfen + Compound of Formula I against insect Whitefly & Shoot and fruit borer population in brinjal crop

[0282] &

[0283]

[0284]

[0285] From the above examples, it is clearly seen that data shown in above Table 1A for percent insect control in brinjal crop that the stable synergistic insecticidal composition of the present invention comprising Isocycloseram + Pyriproxyfen + Compound of Formula I i.e. T1 to T4 are synergistic for insect population with > 1 Colby’s Ratio. The binary combination of two insecticides (Isocycloseram + Pyriproxyfen, Isocycloseram + Compound of Formula I and Pyriproxyfen + Compound of Formula I) T5 to T16 is compatible but no synergistic effect is observed for the control of insect population, as compared to stable synergistic insecticidal composition of the present invention comprising Isocycloseram + Pyriproxyfen + Compound of Formula I i.e. T1 to T4. The synergy data presented herein represent the cumulative efficacy against both whitefly and shoot and fruit borer.Experiment for Bio-efficacy evaluation of stable synergistic insecticidal composition of the present invention:

[0286] For the ternary and binary combination based on Isocycloseram + Pyriproxyfen + Compound of Formula I evaluation under field conditions, the basic test composition as Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC were used. For ternary composition, Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC was used and for binary combinations, Isocycloseram 9.6% + Pyriproxyfen 11.2% SC, Isocycloseram 9.6% + Compound of Formula I 48% SC and Pyriproxyfen 11.2% + Compound of Formula I 48% SC were used. The solo formulations of Isocycloseram 9.6% SC, Pyriproxyfen 11.2% SC and Compound of Formula I 48% SC were used for comparison. The other formulations namely Isocycloseram 9.2% W / W DC (10% W / V) DC, Pyriproxyfen 10 % + BifenthrinlO % w / w EC and Compound of Formula I 50 % WP available in the market were also used for comparison.

[0287] Example 2: Evaluation of the stable synergistic insecticidal composition of the present invention on Brinjal

[0288] The stable synergistic insecticidal composition of the present invention comprising Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC was evaluated on brinjal crop variety Pusa Hybrid 5. The crop sowing was done in Randomized Block Design (RBD) with three replications maintaining a distance of 75 cm x 45 cm. The observations were recorded for the following objectives.

[0289] Objectives:

[0290] 1. Bio-efficacy evaluation against whitefly on Brinjal crop.

[0291] 2. Bio-efficacy evaluation against shoot and fruit borer on Brinjal crop.

[0292] 3. Bio-efficacy evaluation based on percent reduction in whitefly population.

[0293] 4. Bio-efficacy evaluation based on percent reduction in shoot and fruit borer infestation. 5. Bio-efficacy evaluation based on crop yield.

[0294] 6. Phytotoxicity evaluation on Brinjal crop.

[0295] Treatment details:

[0296]

[0297] Methodology:

[0298] The treatments T1 to T18 and T20 were used as foliar application whereas no treatment was done for T19 i.e., control treatment. All treatments were applied at the time of initiation of pest in the experimental plots.

[0299] Whitefly (Bemisia tahaci) incidence is recorded by counting the number of adult whiteflies on the top, middle, and bottom leaves of five randomly selected plants per plot, preferably during early morning hours, and the average population per leaf is calculated before and after treatment. Shoot and fruit borer {Leucinodes orbonalis) infestation is observed by counting the total number of shoots and fruits and the number of damaged shoots and fruits from the same selected plants ateach observation. The percent reduction in insect population was also calculated over control. The crop yield was recorded at crop harvest. The observations for phytotoxicity on Brinjal crop at 1, 3, 7, 10 and 14 days after 1stspray were also recorded. The data were subjected to statistical analysis of variance. Results are presented in Table 2A, 2B, 2C and 2D.

[0300] Results:

[0301] Table 2A: Field bio-efficacy evaluation of stable synergistic insecticidal composition of the present invention comprising Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula 148% SC against whitefly of brinjal crop.

[0302]

[0303]

[0304] Figures in parentheses are square root transformed values; NS= Non-significant

[0305] Table 2B: Field bio-efficacy evaluation of stable synergistic insecticidal composition of the present invention comprising Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC against shoot and fruit borer of brinjal crop.

[0306]

[0307]

[0308]

[0309] Figures in parentheses are arc sine transformed values; NS= Non-significant

[0310] Table 2C: Field bio-efficacy evaluation of stable synergistic insecticidal composition of the present invention comprising Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC based on fruit yield of brinjal crop.

[0311]

[0312]

[0313] t / ha- tons per hectare

[0314] Table 2D: Field bio-efficacy evaluation based on phytotoxicity of stable synergistic insecticidal composition of the present invention comprising Isocy closeram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC on brinjal crop.

[0315]

[0316]

[0317]

[0318] From the given examples, it is clearly seen that the stable synergistic insecticidal composition of the present invention is superior for controlling the whitefly {Bemisia tabaci) and shoot and fruit borer {Leucinodes orbonalis) in Brinjal at 14 days after 1st& 2ndspray (Table 2A and 2B) in Brinjal crop as compared to the other pesticides such as Isocycloseram 9.2% WAV De (10% W / V) DC, Pyriproxyfen 10 % + BifenthrinlO % w / w EC and Compound of Formula I 50 % WP available in the market.

[0319] The yield of Brinjal crop also improved in the stable synergistic insecticidal composition of the present invention comprising Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC as compared to binary and solo compositions (Table 2C).

[0320] The disclosed stable synergistic insecticidal composition of the present invention comprising Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC @ 468.75, 625, 781.25 and 1250 g / ha further showed no phytotoxicity in the Brinjal crop even after 1, 3, 7, 10 and 14 days after 1stspray (Table 2D).

[0321] It is evident from the above data that the stable synergistic insecticidal composition of the present invention i.e., Isocycloseram 9.6% + Pyriproxyfen 11.2% + Compound of Formula I 48% SC resulted in good control of whitefly {Bemisia tabaci) and shoot and fruit borer {Leucinodes orbonalis) in Brinjal with higher respective yields as compared to the reference standard products (binary compositions and solo products). Further, the composition of present invention is resulted synergistic.

[0322] In particular, it has now been found, surprisingly that, for example, the insecticidal activity of the composition of present invention, compared with the insecticidal activity of individual components, is synergistic. In fact, various advantageous properties associated with the compositions according to the invention, include but are not limited to: knock down effect on insect pests at all developmental stages of insects such as egg, nymph, larva, pupa, and adult, preventing discoloration of leaves of plants and crops, enhanced tenderness to plants and crops, preventing ageing of plants and crops and other advantages familiar to a person skilled in the art.From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.

Claims

I / WE CLAIM

1. A stable synergistic insecticidal composition comprising:A) Compound of formula I i.e. N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, isomers, esters and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters and derivatives.

2. The stable synergistic insecticidal composition as claimed in claim 1, comprising:A) Compound of formula I i.e. N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, present in the range of 0.001-90% by weight of the composition;B) Isocycloseram or its agrochemically acceptable salts, isomers, esters and derivatives, present in the range of 0.001-90% by weight of the composition; andC) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters and derivatives, present in the range of 0.001-90% by weight of the composition.

3. A stable synergistic insecticidal composition comprising:A) Compound of formula I i.e. N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, isomers, esters and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters and derivatives; and D) At least one or more additive.

4. A stable synergistic insecticidal composition comprising:A) Compound of formula I i.e. N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, isomers, esters and derivatives; C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters and derivatives;andD) At least one or more additive selected from Urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and Epoxide soybean oil either alone or in combination thereof.

5. A stable synergistic insecticidal composition comprising:A) Compound of formula I i.e. N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives, present in the range of 0.001-90% by weight of the composition;B) Isocycloseram or its agrochemically acceptable salts, esters and derivatives, present in the range of 0.001-90% by weight of the composition;C) Pyriproxyfen or its agrochemically acceptable salts, esters and derivatives, present in the range of 0.001-90% by weight of the composition; andD) At least one or more additive selected from urea clathrate, Siloxane Polyalkyleneoxide Copolymer, tallow amine ethoxylate, ammonium sulphate, and epoxide soybean oil either alone or in combination thereof, in the range of 0.001-20% by weight of the composition.

6. A stable synergistic insecticidal composition comprising:A) Compound of Formula I, i.e., N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'- (l,l-dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, esters, isomers and derivatives;C) Pyriproxyfen or its agrochemically acceptable salts, esters or its derivatives; and D) Protein-based dispersant and / or a polysaccharide -based dispersant.[Claim?] The stable synergistic insecticidal composition as claimed in claims 1-6, which further comprises one or more agrochemically acceptable excipients.

8. The stable synergistic insecticidal composition as claimed in claim 1 to 7, wherein agrochemically acceptable excipients comprises surfactant / s), dispersing agent(s), codispersant, anti-freezing agent(s), emulsifier(s)( (anionic and non-ionic), anti-foaming agent / defoamer(s), wetting agent(s), biocide / antimicrobial / anti-bacterial agent(s), thickening agent / thickener(s), quick coating agent(s) or sticking agents / sticker(s), spreader(s), binder(s),pH-adjusting agent(s), anti-caking agent(s), adjuvant(s), filler(s) / suspension aid(s), color an t(s) / colouring agent(s) / dye(s), carrier(s), stabilizer, buffering agent(s), polymer(s), rheology modifier(s), solvent(s), co-solvent(s), or mixtures thereof.

9. The stable synergistic insecticidal composition as claimed in any one of the claims 1 to 7, wherein the composition is in form of Flowable concentrate for seed treatment (FS), Emulsifiable concentrate (EC), Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Granules (GR Micro-emulsion concentrate (MEC), Oildispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed composition of CS and SC (ZC) or a mixed composition of CS and SE (ZE) and a mixed composition of CS and EW (ZW).

9. . The stable synergistic insecticidal composition as claimed in claims 1 to 8, wherein the use of said composition promotes plant health, growth and increases plant yield in the field.

10. . A process for preparing a stable synergistic insecticidal composition comprising:A) Compound of formula I i.e. N-[2,6-bis(l-methylethyl)-4-phenoxyphenyl]-N'-(l,l- dimethylethyl)thiourea or its agrochemically acceptable salts, esters and derivatives; B) Isocycloseram or its agrochemically acceptable salts, isomers, esters and derivatives; and C) Pyriproxyfen or its agrochemically acceptable salts, isomers, esters and derivatives.

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