Organic agricultural input product and system and method of making same

Abstract

Disclosed is an all-natural organic agricultural input composition and a system and methos for making it. More specifically; disclosed is a product having plant biostimulant, biopesticide, soil conditioning, and bioadjuvant properties for improving crop yield and nutrient density, and the bioreactor system and fermentation process for making the product.

Description

Atty Docket LZ0003PCTORGANIC AGRICULTURAL INPUT PRODUCT AND SYSTEM AND METHOD OF MAKING SAMESUMMARY

[0001] In a first aspect, a liquid agricultural input product is provided. In one embodiment, the liquid agricultural input product contains plant hormones, secondary metabolites from fermentation and respiration, plant micronutrients and macronutrients, biogenic amines, enzymes to facilitate nutrient uptake, sugars and sugar alcohols, and amino acids

[0002] In one embodiment, the liquid agricultural input product does not contain particles of a size greater than 100 microns.

[0003] In one embodiment, the liquid agricultural input product has a pH that is less than 6, total solids less than 20% by weight, and more than 10 grams per liter of total organic carbon.

[0004] In one embodiment, the liquid agricultural input product has biostimulant, biopesticide, soil conditioning, and / or adjuvant activity or function

[0005] In a second aspect, a method for making the liquid agricultural input product of the first aspect is provided. In one embodiment, the method includes the steps of combining in a vessel molasses and fish meal in water, pasteurizing the combination, adding gram positive bacteria after pasteurizing, allowing the mixture to incubate with mixing in the presence of air for a time at a permissive temperature for the bacteria, and then separating the solid material from the liquid, wherein the liquid portion is the liquid agricultural input product.

[0006] In a third aspect, a system for making the liquid agricultural input product of the first aspect is provided.

[0007] In a fourth aspect, a method of using the liquid agricultural input product of the first aspect is provided. In one embodiment, the product is diluted and applied to soil before planting, at plant emergence, continuously during the life cycle, and / or once per week.

[0008] In one embodiment, the product is diluted and sprayed onto the plant stem and leaves.

[0009] In one embodiment, the product-treated soil on which the plants are grown or the product-treated plants show improved growth rate, improved size, improved yield, improved nutrient uptake, improved nutrient accumulation, and / or improved protein levels compared to untreated soil or plants.Atty Docket LZ0003PCT

[0010] In one embodiment, the product is used to coat seeds, such that germination rate, germination timing, and / or radical length is improved relative to seeds that are not coated with product.DRAWINGS

[0011] Figure 1 is a flow chart depicting the steps for making a multi-effect plant growth input composition (the product),

[0012] Figure 2 is a line graph depicting the effect of the product over time on the pH of soil planted with basil. The X-axis represents time in days. The Y-axis represents pH. The solid line represents no-product water control (Group 1 ); the deashed line represents group with product applied to soil pre-planting only (Group 2), the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0013] Figure 3 is a line graph depicting the effect of the product over time on the pH of soil planted with marigolds. The X-axis represents time in days. The Y-axis represents pH. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3),

[0014] Figure 4 is a line graph depicting the effect of the product over time on the pH of soil planted with alfalfa. The X-axis represents time in days. The Y-axis represents pH. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0015] Figure 5 is a line graph depicting the effect of the product over time on the pH of soil planted with wheat. The X-axis represents time in days. The Y-axis represents pH. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).Atty Docket LZ0003PCT

[0016] Figure 6 is a line graph depicting the effect of the product over time on the humidity or water-retention of soil planted with basil. The X-axis represents time in days. The Y-axis represents percent humidity of the soil. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0017] Figure 7 is a line graph depicting the effect of the product over time on the humidity or water-retention of soil planted with marigolds. The X-axis represents time in days. The Y- axis represents percent humidity’ of the soil. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3),

[0018] Figure 8 is a line graph depicting the effect of the product over time on the humidity or water-retention of soil planted with alfalfa. The X-axis represents time in days. The Y-axis represents percent humidity of the soil. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0019] Figure 9 is a line graph depicting the effect of the product over time on the humidity or water-retention of soil planted with wheat. The X-axis represents time in days. The Y-axis represents percent humidity of the soil. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0020] Figure 10 is a line graph depicting the effect of the product over time on the growth rate of wheat as measured by leaf length. The X-axis represents time in days. The Y-axis represents leaf height in centimeters. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0021] Figure 11 is a line graph depicting the effect of the product over time on the growth rate of marigold as measured by plant height. The X-axis represents time in days. The Y-axisAtty Docket LZ0003PCTrepresents plant height in centimeters. The solid line represents no-product water control (Group 1); the deashed line represents group with product applied to soil pre-planting only (Group 2); the dotted line represents group with product applied to soil pre-planting and subsequent weekly application of product to soil (Group 3).

[0022] Figure 12 is a box and whiskers graph depicting wheat leaf length in centimeters (plant height) at day 15 post-seeding. From left to right, the black box represents the no-product control group 1 (1Wi, 1Wii, 1Wiii, 1Wiv, and 1Wv), the dark gray box represents group 2 with soil soaked with product prior to seeding (2Wi, 2Wii, 2Wiii, 2Wiv, and 2Wv), the light gray box represents group 3 with soil soaked with product prior to seeding and weekly application of product to the soil (3Wi, 3 Wii, 3Wiii, 3Wiv, and 3 Wv).

[0023] Figure 13 is a box and whiskers graph depicting wheat shoot wet mass in grams at day 15 post-seeding. From left to right, the black box represents the no-product control group 1 (1Wi, 1Wii, 1Wiii, 1Wiv, and 1Wv). the dark gray box represents group 2 with soil soaked with product prior to seeding (2Wi, 2Wii, 2Wiii, 2Wiv, and 2Wv), the light gray box represents group 3 with soil soaked with product prior to seeding and weekly application of product to the soil (3Wi, 3Wii, 3Wiii, 3Wiv, and 3Wv).DETAILED EMBODIMENTS

[0024] Several embodiments are herein described.

[0025] Embodiment 1 provides a liquid agricultural input composition (the product) comprising a phytohormone, a secondary metabolite, an effective microbe, a plant macronutrient, a plant micronutrient, a biogenic amine, an enzyme, a sugar, a sugar alcohol, an amino acid, and water.

[0026] Embodiment 2 provides a composition of embodiment 1 comprising at least two phytohormones, at least two secondary metabolites, at least two effective microbes, at least two plant macronutrients, at least two biogenic amines, at least two enzymes, at least two sugars, at least two sugar alcohols, and at least two amino acids.

[0027] Embodiment 3 provides a composition of embodiment 1 or 2 that does not comprise a suspended solid greater than 100 microns in diameter.

[0028] Embodiment 4 provides a composition of any one of embodiments 1 -3 comprising 6% - 10% solids.Atty Docket LZ0003PCT

[0029] Embodiment 5 provides a composition of any one of embodiments 1 -4 comprising 2% - 6% total carbon.

[0030] Embodiment 6 provides a composition of any one of embodiments 1-3 having a pH of 3 — 6.

[0031] Embodiment 7 provides a composition of any one of embodiments 1-6, wherein the phytohormone is selected from the group consisting of a gibberellin or derivative thereof, an auxin or derivative thereof, a cytokinin or derivative thereof, and an abscisic acid or derivative thereof.

[0032] Embodiment 8 provides a composition of any one of embodiments 1-7. wherein the secondary metabolite is selected from the group consisting of lactic acid, succinic acid, shikimic acid, 2,3-butanediol, tricarballylic acid, and 3-deoxyhexonic acid.

[0033] Embodiment 9 provides a composition of any one of embodiments 1-8, wherein the effective microbe is selected from the group consisting of Lactobacillus sp., Oceanobacillus sp.. Bacillus sp., Myroides sp.. Terribacillus sp.. Aneurinibacillus sp.. Paenibacillus sp., and Lysinibacillus sp.

[0034] Embodiment 10 provides a composition of any one of embodiments 1-9, wherein the plant macronutrient is selected from the group consisting of nitrogen, phosphorous, potassium, calcium, magnesium, and sulfur.

[0035] Embodiment 11 provides a composition of any one of embodiments 1-10. wherein the plant micronutrient is selected from the group consisting of zinc, chlorine, boron, molybdenum, iron, and manganese.

[0036] Embodiment 12 provides a composition of any one of embodiments 1-11 wherein the biogenic amine is selected from the group consisting of cadaverine, putrescine, tyramine. and ethanolamine.

[0037] Embodiment 13 provides a composition of any one of embodiments 1-12. wherein the enzyme is selected from the group consisting of phosphatase, cellulase, and nitrogenase.

[0038] Embodiment 14 provides a composition of any one of embodiments 1-13. wherein the sugar is selected from the group consisting of trehalose, tagatose, fructose, sucrose, glucose, glucopyranose, mannose, maltose, xylose, lactose, galactose, and kestose.Atty Docket LZ0003PCT

[0039] Embodiment 15 provides a composition of any one of embodiments 1-14, wherein the sugar is selected from the group consisting of erythritol, inositol, glycerol, mannitol, ribitol, sorbitol, and adonitol.

[0040] Embodiment 16 provides a composition of any one of embodiments 1-15, wherein the amino acid is selected from the group consisting of pyroglutamic acid, gamma amino butyric acid, valine, aspartic acid, 5-aminovaleric acid, and serine.

[0041] Embodiment 17 provides a composition of any one of embodiments 1-16 comprising 1 ng / g - 400 ng / g of abscisic acid or metabolites thereof.

[0042] Embodiment 18 provides a composition of any one of embodiments 1-17 comprising 1 ng / g - 20 ng / g of abscisic acid

[0043] Embodiment 19 provides a composition of any one of embodiments 1-18 comprising 0.5 ng / g – 40 ng / g of cytokinin.

[0044] Embodiment 20 provides a composition of any one of embodiments 1-19 comprising 20 ng / g - 80 ng / g of indole acetic acid (IAA).

[0045] Embodiment 21 provides a composition of any one of embodiments 1-20 comprising 0.5 ng / g – 25 ng / g of gibberellin.

[0046] Embodiment 22 provides a composition of any one of embodiments 1-21 comprising lactic acid and 2,3-butanediol.

[0047] Embodiment 23 provides a composition of any one of embodiments 1-22 comprising 3-deoxyhexonic acid and succinic acid.

[0048] Embodiment 24 provides a composition of any one of embodiments 1-23 comprising tricarballylic acid and shikimic acid.

[0049] Embodiment 25 provides a composition of any one of embodiments 1-24 comprising Lactobacillus gallinarum and Lactobacillus johnsonii.

[0050] Embodiment 26 provides a composition of any one of embodiments 1-25 comprising Bacillus subtilis and Paenibacillus polymyxa.

[0051] Embodiment 27 provides a composition of any one of embodiments 1-26 comprising no more than 2% total Kjeldahl nitrogen.Atty Docket LZ0003PCT

[0052] Embodiment 28 provides a composition of any one of embodiments 1-27 comprising 0.05% - 0.5% total Kjeldahl nitrogen.

[0053] Embodiment 29 provides a composition of any one of embodiments 1-28 comprising no more than 2% phosphate.

[0054] Embodiment 30 provides a composition of any one of embodiments 1-29 comprising 0.1% - 0.5% phosphate.

[0055] Embodiment 31 provides a composition of any one of embodiments 1-30 comprising no more than 2% potash.

[0056] Embodiment 32 provides a composition of any one of embodiments 1-31 comprising 0.1% - 1% potash.

[0057] Embodiment 33 provides a composition of any one of embodiments 1-32 comprising no more than 1 % sulfur.

[0058] Embodiment 34 provides a composition of any one of embodiments 1-33 comprising 0.05% - 0.2% sulfur.

[0059] Embodiment 35 provides a composition of any one of embodiments 1-34 comprising no more than 1 % calcium.

[0060] Embodiment 36 provides a composition of any one of embodiments 1-35 comprising 0.1% - 0.6% calcium.

[0061] Embodiment 37 provides a composition of any one of embodiments 1-36 comprising no more than 1% magnesium.

[0062] Embodiment 38 provides a composition of any one of embodiments 1-37 comprising 0.03% - 0.09% magnesium.

[0063] Embodiment 39 provides a composition of any one of embodiments 1-38 comprising no more than 500 ppm iron.

[0064] Embodiment 40 provides a composition of any one of embodiments 1-39 comprising 50 ppm - 300 ppm iron.

[0065] Embodimen t 41 provides a composition of any one of embodiments 1-40 comprising no more than 100 mg / kg zinc.Atty Docket LZ0003PCT

[0066] Embodiment 42 provides a composition of any one of embodiments 1-41 comprising 1 mg / kg - 10 mg / kg zinc.

[0067] Embodiment 43 provides a composition of any one of embodiments 1-42 comprising at least 10,000 mg / L of total organic carbon

[0068] Embodiment 44 provides a composition of any one of embodiments 1-43 comprising 15,000 mg / L - 40,000 mg / L of total organic carbon.

[0069] Embodiment 45 provides a composition of any one of embodiments 1-44 having a carbon to nitrogen ratio (C:N) of at least 2:1.

[0070] Embodiment 46 provides a composition of any one of embodiments 1-45 having a C:N of 5:1 – 25:1.

[0071] Embodiment 47 provides a composition of any one of embodiments 1-46 comprising cadaverine and putrescine.

[0072] Embodiment 48 provides a composition of any one of embodiments 1-47 comprising phosphatase and nitrogenase.

[0073] Embodiment 49 provides a composition of any one of embodiments 1-48 comprising fructose, trehalose, and sucrose.

[0074] Embodiment 50 provides a composition of any one of embodiments 1-49 comprising mannitol, inositol, and glycerol.

[0075] Embodiment 51 provides a composition of any one of embodiments 1-50 comprising pyroglutamic acid and gamma aminobutyric acid (GABA).

[0076] Embodiment 52 provides a composition of any one of embodiments 1-51. wherein said composition is a plant biostimulant.

[0077] Embodiment 53 provides a composition of any one of embodiments 1-52, wherein said composition is a soil conditioner or amendment.

[0078] Embodiment 54 provides a composition of any one of embodiments 1-53, wherein said composition is a biopesticide.

[0079] Embodiment 55 provides a composition of any one of embodiments 1-54. wherein said composition is a bioadjuvant.Atty Docket LZ0003PCT

[0080] Embodiment 56 provides a method of making a liquid agricultural input of any one of embodiments 1-55, the method comprising (a) in a vessel, combining an animal byproduct with a plant byproduct in water to form a first suspension, (b) pasteurizing the first suspension to form a pasteurized suspension, (c) adding gram-positive bacteria to the pasteurized suspension to form a bioreaction suspension, (d) incubating the bioreaction suspension to form a fermentation product suspension, and (e) removing solid material from the fermentation product suspension to form a liquid agricultural input.

[0081] Embodiment 57 provides a method of embodiment 56 further comprising sparging air into the vessel after the pasteurizing and during the incubating.

[0082] Embodiment 58 provides a method of embodiment 56 or 57 further comprising mixing the first suspension, the pasteurized suspension, the bioreaction suspension, and the fermentation product suspension.

[0083] Embodiment 59 provides a method of embodiment 58, wherein said mixing is continuous

[0084] Embodiment 60 provides a method of embodiment 58. wherein said mixing is discontinuous.

[0085] Embodiment 61 provides a method of any one of embodiments 56-60, wherein the pasteurizing is at > 55°C for > 24 hours.

[0086] Embodiment 62 provides a method of any one of embodiments 56-61, wherein the gram-positive bacteria having an OD600 of 0.5 - 1.8 or having a titer of 1E6 cfu / 'mL - 3E8 cfu / mL is added to the pasteurized suspension at a dilution of 1:10 - 1:500.

[0087] Embodiment 63 provides a method of any one of embodiments 56-62. wherein the incubating is at 30°C -,50c’C for 24 hours - 96 hours,

[0088] Embodiment 64 provides a method of any one of embodiments 56-63, wherein the solid material is removed by passing the suspension over a 100 mesh - 300 mesh screen and retaining the liquid filtrate.

[0089] Embodiment 65 provides a method of any one of embodiments 56-64, wherein the solid material is removed by centrifuging the suspension and retaining the liquid supernatant.

[0090] Embodiment 66 provides a method of any one of embodiments 56-65, wherein the plant byproduct is molasses.Atty Docket LZ0003PCT

[0091] Embodiment 67 provides a method of any one of embodiments 56-66, wherein the plant byproduct is at 5%-15% by weight in the first suspension.

[0092] Embodiment 68 provides a method of any one of embodiments 56-67. wherein the plant byproduct is at 10% by weight in the first suspension.

[0093] Embodiment 69 provides a method of any one of embodiments 56-68, wherein the animal byproduct is fish meal.

[0094] Embodiment 70 provides a method of any one of embodiments 56-69, wherein the animal byproduct is at 1 %-10% by weight in the first suspension.

[0095] Embodiment 71 provides a method of any one of embodiments 56-70, wherein the animal byproduct is at 5% by weight in the first suspension

[0096] The method of any one of embodiments 56-71, wherein the gram-positive bacteria is a facultative anerobic firmicutes.

[0097] The method of any one of embodiments 56-72, wherein the gram-positive bacteria is Paenibacillus polymyxa.

[0098] Embodiment 74 provides a system for producing a liquid agricultural input of any one of embodiments 1-55 (the product) or of performing the method of any one of embodiments 56-73, the system comprising a vessel having a top, a bottom, and sides enclosing an inner cavity from an outside environment, a mixer, an air pump, and a temperature regulator, configured to hold a suspension comprising solid material suspended in liquid material within the inner cavity.

[0099] Embodiment 75 provides a system of embodiment 74. wherein the vessel is a tank or drum.

[0100] Embodiment 76 provides a system of embodiment 74 or 75, wherein the vessel is a drum.

[0101] Embodiment 77 provides a system of embodiment 74 or 75, wherein the vessel is a polymer tank having a capacity' of at least 450 gallons.

[0102] Embodiment 78 provides a system of any one of embodiments 74-77, wherein the mixer is a submersible pump situated in the inner cavity and at the bottom of the vessel andAtty Docket LZ0003PCTconfigured to move the suspension from the bottom to the top of the vessel within the cavity of the vessel.

[0103] Embodiment 79 provides a system of any one of embodiments 74-78. wherein the air pump is a regenerative blower situated outside of the vessel and configured to inject air through a pipe into the inner cavity proximate the bottom of the vessel.

[0104] Embodiment 80 provides a system of any one of embodiments 74-79, wherein the temperature regulator is a heater.

[0105] Embodiment 81 provides a system of any one of embodiments 74-80, wherein the temperature regulator is a thermostatically controlled electric blanket configured to wrap around the outside of the vessel.

[0106] Embodiment 82 provides a system of any one of embodiments 74-81, wherein the temperature regulator is an IBC tote warming blanket wrapped around the outside of the vessel.

[0107] Embodiment 83 provides a system of any one of embodiments 74-82 further comprising a liquid separator configured to receive the suspension from the vessel and to separate the solid material from the liquid material.

[0108] Embodiment 84 provides a system of embodiment 83, wherein the separator is a vibration separator.

[0109] Embodiment 85 provides a system of embodiment 83 or 84, wherein the separator is a vibration separator comprising a 180 mesh - 240 mesh sieve,

[0110] Embodiment 86 provides a method for conditioning soil for use in agriculture, horticulture, or potted plants comprising applying to the soil a liquid agricultural input composition ( the product) of any one of embodiments 1-55.

[0111] Embodiment 87 provides method of embodiment 86, wherein the product is diluted 1 part product to from 3 parts diluent to 100 parts diluent (1:3 – 1:100, product:diluent).

[0112] Embodiment 88 provides a method of embodiment 86 or 87, wherein the product is diluted 1:3 in water or an aqueous solution and applied to the soil of a potted plant.Atty Docket LZ0003PCT

[0113] Embodiment 89 provides a method of embodiment 86 or 87, wherein the product in diluted form is applied to a garden plot at a rate of about 1 gallon per acre to about 1 quart per 1,000 square feet.

[0114] Embodiment 90 provides a method of embodiment 86, 87, or 89, wherein the product in diluted form is applied to a field at a rate of about 1 gallon to 3 gallons per acre.

[0115] Embodiment 91 provides a method of embodiment 86, 87, 89, or 90, wherein the product in diluted form is applied to a field at a rate of about 2 gallons per acre.

[0116] Embodiment 92 provides a method of any one of embodiments 86-91, wherein the humidity or water holding capacity of the soil is improved.

[0117] Embodiment 93 provides a method of any one of embodiments 86-92, wherein the soil moisture is increased by at least 40% relative to soil without product.

[0118] Embodiment 94 provides a method of any one of embodiments 86-93, wherein the pH of the soil is less than 7.5.

[0119] Embodiment 95 provides a method of any one of embodiments 86-94, wherein the soil pH is decreased by at least 5% relative to soil without product.

[0120] Embodiment 96 provides a method of any one of embodiments 86-95, wherein plants grown in the conditioned soil grow’ by height at a rate that is at least 8% greater than the growth rate of similar plants grow n w ithout application of the product to the soil.

[0121] Embodiment 97 provides a method of any one of embodiments 86-96, wherein plants grown in the conditioned soil grow by wet shoot mass at a rate that is at least 20% greater than the grow th rate of similar plants grown without application of the product to the soil,

[0122] Embodiment 98 provides a method of any one of embodiments 86-97, w herein plants grown m the conditioned soil produce at least 40% more protein than plants grown without application of the product to the soil.

[0123] Embodiment 99 provides a method of any one of embodiments 86-98, w’herein plants grown in the conditioned soil accumulate at least 150% more phosphorous than plants grown without application of the product to the soil.Atty Docket LZ0003PCT

[0124] Embodiment 100 provides a method of any one of embodiments 86-99, wherein plants grown in the conditioned soil accumulate at least 40% more nitrogen than plants grown without application of the product to the soil.

[0125] Embodiment 101 provides a method of any one of embodiments 86-100, wherein plants grown in the conditioned soil accumulate at least 10% more iron than plants grown without application of the product to the soil.

[0126] Embodiment 102 provides a method of any one of embodiments 86-101, wherein plants grown in the conditioned soil accumulate at least 50% more zinc than plants grown without application of the product to the soil.EXAMPLESEXAMPLE 1: PROCESSING

[0127] A 500-gallon cone-bottom tank was filled with 250 gallons of filtered water In some process embodiments, the tank was charged with filtered water heated to 120-140°F 564 pounds of molasses and 282 pounds of fishmeal were added to the tank with stirring. The tank was topped off with filtered water to a final volume of 450 gallons.

[0128] The tank was heated to 55°C with continuous stirring and held at 55°C for 24 hours to pasteurize the contents.. After pasteurization, the tank contents were cooled to at least 46°C and about 1.5L-20L of 1E8-1E9 cfu / mL of Paenibacillus polymyxa was added to the tank. The tank contents were allowed to ferment for 72 hours - 120 hours without additional heat and then harvested

[0129] The contents of the tank w ere harvested by gravity after 72-120 hours. The pH of the contents at harvesting was about 4.5 - 6. The temperature of the contents at harvest was about 30°C - 35°C. The TDS of the contents at harvest w as about 6000 - 12000.

[0130] The remaining fishmeal and other solids were removed from the harvested contents using a 200 or 230-mesh vibration screen to remove particles having an average diameter of 60-75 microns. The remaining liquid (product of the composition or a.k.a. Verdante) w as (a) analyzed for plant hormones, fermentation products, nutrient salts, and microbial load, (b) stored at 12-17°C, and packaged for shipping.Atty Docket LZ0003PCT

[0131] The contents of the tank were continuously stirred throughout the process using a submersible sewage pump positioned at the bottom of the tank within the cone. The tank was continuously monitored throughout the process for pH, temperature, and total dissolved solids (TDS) using a WiFi enabled submersible probe.EXAMPLE 2: PRODUCT ANALYSIS

[0132] The product (a.k.a. Verdante) was subjected to anonymous mass spectroscopy metabolomics and the major products reported were 2.3-butanediol, succinic acid, 1,2,3-butanetriol, tricarballylic acid, cadaverine, myo-inositol, 1 -octadecane, pyroglutamic acid, vanillylmandelic acid, glutaric acid, tyramine, 1-methyl-hydantoin, putrescine, sucrose, L-valine, trimethoxy mandelic acid, raffinose, lactic acid, hydroxymandelic acid, viburtinol, glycolic acid, hexanoic acid, hydrocinnamic acid, L-leucine, ribonic acid, phosphoric acid, uracil, pentanoic acid, hydroxy benzoic acid, gamma-aminobutyric acid, vanillic acid, glycine, glycerol, fructose, L-isoleucine, pentane-l,5-diamine, and others. 2,3-butanediol was reported as a major component.

[0133] The product was subjected to plant hormone analysis and observed to contain about 150-320 ng / g of abscisic acid, about 0.5-40 ng / g cytokinins, about 20-80 ng / g auxins, and about 0.2-25 ng / g gibberellins.

[0134] Liquid product samples were quantitatively analyzed for the measurement of ABA and ABA metabolites, cytokinins, auxins, and gibberellins by using UPLC ESI-MS / MS. Deuterated forms of the hormones were used as internal standards: d4-ABA, d5-ABA-GE, d3-DPA, d3-PA, d4-7'-OH-ABA, d3-neoPA, d4-trans-ABA; d5-IAA, d3-IAA-Asp, d3-IAA-Glu, d3-IAA-Ala, d3-IAA-Leu, 13C4-IBA; d5-Z-O-Glu, d3-dhZ, d.3-dhZR, d6-iP, d6-iPR, 15N4-kinetin; d2-GAs 1, 3, 4, 7, 8, 9, 19, 20, 24, 29, 34, 44, 51 and 53. Calibration curves were created for all compounds of interest. Quality control samples (QCs) were run along with the samples. Analysis was performed on a UPLC / ESI-MS / MS utilizing a Waters ACQUITY UPLC system, equipped with a binary' solvent delivery' manager and a sample manager coupled to a Waters MICROMASS QUATTRO PREMIER XE quadrupole tandem mass spectrometer via a Z-spray interface (Waters, Millford, MA). MASSLYNX™ and QUANLYNX™ (Micromass, Manchester. UK) were used for data acquisition and data analysis.Atty Docket LZ0003PCT

[0135] The quantitative analysis utilized the Multiple Reaction Monitoring (MRM) function of the MASSLYNX v4.1 control software. The resulting chromatographic traces for each analyte (endogenous phytohormone) and their respective deuterium labeled internal standard were quantified off-line using the QUANLYNX v4.1 software, wherein each trace was integrated and the resulting ratio of signals (analyte / intemal standard) were compared with a previously constructed calibration curve to yield the amount of analyte present (ng per sample). Calibration curves were generated from the MRM signals obtained from standard solutions based on the ratio of the chromatographic peak area for each analyte to that of the corresponding internal standard. The QC samples, internal standard blanks and solvent blanks were also prepared and analyzed along each batch of samples.

[0136] To determine the identity and relative amounts of microbes present in the product, next generation sequencing (NGS)was performed on product samples. The samples were processed and analyzed with the Microbiome Sequencing Service 16S / ITS Amplicon Sequencing (Zymo Research, Irvine, CA).

[0137] The DNA samples were prepared for targeted sequencing with the Quick-16S™ Plus NGS Library Prep Kit (Zymo Research, Irvine, CA). These primers were custom designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity. Primers used were QUICK-16S™ PRIMER SET V3-V4 (Zymo Research. Irvine, CA)10138 ] The sequencing library was prepared using PCR reactions performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity. The final pooled library was cleaned up with the SELECT-A-SIZE DNA CLEAN & CONCENTRATOR™ (Zymo Research, Irvine, CA), then quantified with TapeStation® (Agilent Technologies, Santa Clara, CA) and Invitrogen QUBIT 1X DSDNA HIGH-SENSITIVITY ASSAY KITS ® (Thermo Fisher Scientific. Waltham, WA).

[0139] The ZymoBIOMICS® Microbial Community Standard (Zymo Research, Irvine, CA) was used as a positive control for each DNA extraction and for the targeted library preparation Negative controls (i.e blank extraction control, blank library' preparation control) were included to assess the level of bioburden carried by' the wet-lab process. The final library was sequenced on Illumina® NextSeq 2000™ with a pl (cat 20075294) reagent kit (600 cycles). The sequencing was performed with 30% PhiX spike-inAtty Docket LZ0003PCT

[0140] Unique amplicon sequences were inferred from raw reads using the Dada2 pipeline (Callahan et al., 2016). Chimeric sequences were also removed with the Dada2 pipeline. Taxonomy assignment was performed using Uclust from Qiime v.1.9.1 Taxonomy was assigned with the Zymo Research Database, a 16S database that is internally designed and curated, as reference. Composition visualization, alpha-diversity, and beta-diversity analyses were performed with Qiime v.1.9.1 (Caporaso et al.. 2010). If applicable, taxonomy that have significant abundance among different groups were identified by LEfSe (Segata et al., 2011) using default settings. Other analyses such as heatmaps, Taxa2SV_deomposer, and PCoA plots were performed with internal scripts.

[0141] A quantitative real-time PCR was set up with a standard curve. I’he standard curve was made with plasmid DNA containing one copy of the 16S gene and one copy of the fungal ITS2 region prepared in 10-fold serial dilutions. The primers used 'ere the same as those used in Targeted Library. The equation generated by the plasmid DNA standard curve was used to calculate the number of gene copies in the reaction for each sample. The PCR input volume (2 µl) was used to calculate the number of gene copies per microliter in each DNA sample.

[0142] The number of genome copies per microliter DNA sample was calculated by dividing the gene copy number by an assumed number of gene copies per genome. The value used for 16S copies per genome is 4. The value used for ITS copies per genome is 200.

[0143] The amount of DNA per microliter DNA sample was calculated using an assumed genome size of 4.64 x 106bp, the genome size of Escherichia coli, for 16S samples, or an assumed genome size of 1.20E7 bp, the genome size of Saccharomyces cerevisiae. for ITS samples.

[0144] The predominant genera found in the product include lactobacillus, bacillus, acetobacter, paenibacillus, The predominant species found in the product include Lactobacillus acetotolerans, Acetobacter fabarum, Pseudomonas psychrophile, Lactobacillus parafarraginis, Lactobacillus salivarus, Lactobacillus buchneri, Lactobacillus parafaringinis, Lactobacillus rhamnosis, Lactobacillus gallinarum, and Paenibacillus polymyxa

[0145] Liquid product samples were quantitatively analyzed for the presence of various nutrients, solids, and total carbon. Hie results are presented in Table 1.

[0146] Table 1Atty Docket LZ0003PCTNutrient / Attribute Amount Range Amount Average Total nitrogen 0.1-0.4% 0.28%Total phosphorous 0.05-0.4% 0.22%Total potassium 0.3-2% 0.96%Iron 0-2% 1%Sulphur 0.05-0.3% 0.1%Calcium 0.05-0.3% 0.19%Magnesium 0.01-0.08% 0.04%Sodium 0.01-0.08% 0.05%Total organic carbon (TOC) 5000 - 25000 mg / L 19000 mg / LTotal solids 3-10% 4.5%Carbon to nitrogen ratio (C: N) 0.2-0.5 0.4pH 4-6 5.2EXAMPLE 3: SOIL CONDITIONING

[0147] The effects of product on soil were tested. Test soil was prepared by combining potting soil, perlite and peat to make the test soil, placing equal amounts of the test soil in 6-inch diameter pots, and then treating the soil with distilled water or 25% product diluted in distilled water. Pots (6-in) were watered daily with 25-100 mL distilled water. Pots / plants were kept at 14-hour lighting, 40-60% humidity. and 70-75°F. Pots were seeded with basil, alfalfa, marigolds, or wheat. Table 2 presents the experimental design. 5 pots were used in each group (N=5).

[0148] Table 2Experimental Treatment Regimen Plant Exp.Group1 Initial soak with water, watered daily Wheat IWi to IWv Alfalfa lAi-lAv Marigold IMi-lMv Basil IBi-lBv2 Initial soak with 25% product, Wheat 2Wi-2Wv watered daily. Alfalfa 2Ai-2Av Marigold 2Mi-2Mv Basil 2Bi-2Bv3 Wheat 3Wi-3WvAtty Docket LZ0003PCTInitial soak with 25% product, Alfalfa 3Ai-3Av watered daily, 25% product applied Marigold 3Mi-3Mvweekly Basil 3Bi-3Bv

[0149] Treatment of soil with product showed a significant reduction in soil pH (see Tables 3-6 and Figures 2-5) and significant increase in water retention (see Tables 7-10 and Figures 6-9). Groups treated with product showed a significant reduction (~5%-10% reduction in pH) of pH to within the ideal range for healthy soil for optimal plant health relative to the control groups, which showed moderately alkaline and unideal pH conditions. Groups treated with product showed a significant increase in water retention as measured by soil humidity (40%-60% increase soil moisture) to within the ideal range for healthy soil for optimal plant health relative to the control groups

[0150] Table 3: Soil pH with basil plants (experimental groups per Table 2)Day pH Group 1 (no pH Group 2 pH Group 3product)8 7.53 7.16 7.139 7.42 6.99 7.2010 7.43 7.00 7.1913 7.53 7.14 7.1915 7.62 7.10 7.0920 7.00 6.88 6.9522 7.07 6.89 6.9729 7.05 7.06 7.0331 7.25 7.11 7.0034 7.53 7.32 6.96

[0151] Table 4: Soil pH with marigold plants (experimental groups per Table 2)Day pH Group 1 pH Group 2 pH Group 38 7.75 7.46 7.129 7.74 7.22 7.0910 7.82 7.53 6.9813 7.95 7.58 7.0615 7.88 7.32 7.0720 7.74 7.18 6.9122 7.60 6.98 6.8829 8.07 7.56 7.0231 8.19 7.68 7.0134 8.09 7.65 7.13

[0152] Table 5: Soil pH with alfalfa plants (experimental groups per Table 2)Atty Docket LZ0003PCTDay pH Group 1 pH Group 2 pH Group 38 7.90 7.39 7.239 7.94 7.21 7.1210 7.84 7.05 6.8913 7.82 7.25 7.0615 7.67 6.99 6.9420 7.63 6.98 6.9022 7.72 7.05 6.9029 7.85 7.37 7.1031 7.94 7.87 7.2134 8.00 8.03 7.38

[0153] Table 6: Soil pH with wheat plants (experimental groups per Table 2)Day pH Group 1 pH Group 2 pH Group 38 7.75 7.40 7.169 7.73 7.27 7.1210 7.73 7.47 7.0313 7.87 7.56 7.1315 7.94 7.71 7.33

[0154] Table 7: Average Soil Moisture (%) of Basil-Planted GroupsDay % Moisture - Group 1 % Moisture - Group 2 % Moisture - Group 3 8 33.83 42.80 43.509 36.67 47.30 43.2010 35.50 49.00 47.9013 34.00 42.90 40.8015 33.50 44.50 43.7020 46.50 56.80 50.3022 44.50 54.00 51.9029 44.17 46.20 48.7031 40.33 44.70 48.6034 34.67 41.40 48.30

[0155] Table 8: Average Soil Moisture (%) of Marigold-Planted GroupsDay % Moisture - Group 1 % Moisture - Group 2 % Moisture - Group 3 8 29.20 36.9 48.39 29.60 41.1 46.710 28.70 34.5 52.613 25.30 32.8 45.315 27.20 37.9 4620 30.40 42.6 54.822 30.30 47.3 56.5Atty Docket LZ0003PCT29 22.20 34.7 47.231 20.20 30.8 46.934 21.60 31.7 44.3

[0156] Table 9: Average Soil Moisture (%) of Alfalfa-Planted GroupsDay % Moisture - Group 1 % Moisture - Group 2 % Moisture - Group 3 8 25.3 37.2 40.19 25.2 40.5 42.910 29 44.9 54.413 27.9 40 44.215 31.3 46.4 50.820 31.6 49.3 57.222 29.9 45.8 56.529 27.7 36.8 42.331 25.6 27.5 39.734 24.1 23.7 37.2

[0157] Table 10: Average Soil Moisture (%) of Wheat-Planted GroupsDay % Moisture - Group 1 % Moisture - Group 2 % Moisture - Group 3 8 29.7 36.4 43.19 30.5 40.3 43.410 30.9 35.1 48.313 27.5 33.8 45.215 26.2 28.4 37.5EXAMPLE 4: PLANT GROWTH ENHANCEMENT

[0158] Soil and potted plants were treated as described m Table 2. The growth of wheat and marigolds were assessed for each group (1 W, 2W, 3W, IM, 2M, 3M) by measuring plant height over time in each group, and wet weight and height of wheat at day 15-16 post¬ seeding.

[0159] The height of wheat leaves was measured each day of the course of 9 days. Wheat treated with product showed greater than 20% greater height / rate of growth compared to untreated wheat (Figure 10, Table 11).

[0160] Table 11: Average wheat leaf length in centimeters over timeDay Height (cm) - Height (cm) - Height (cm) -Group 1 Group 2 Group 3Atty Docket LZ0003PCT7 8.78 7.98 7.348 12.52 11 98 12.009 14.20 13.98 14.3410 15.72 16.34 16.7013 18.34 20.20 22.6015 22.34 26.84 27.94

[0161] The height of marigold plants was measured each day of the course of 28 days. Marigolds treated with product showed greater than 9% greater height / rate of growth compared to untreated marigolds (Figure 11, Table 12).

[0162] Table 12: Average marigold plant height in centimeters over timeDay Height (cm) - Height (cm) - Height (cm) - Group 1 Group 2 Group 37 1.78 1.86 1.808 2.08 2.04 2.129 2.34 2.30 2.4010 2.48 2.34 2.6813 2.94 2.72 3.4815 3.28 3.62 4.1029 4.40 5.16 7.0431 5.00 5.98 7.1034 5.70 6.26 7.86EXAMPLE 5: ENHANCED NUTRIENT UPTAKE AND PROTEIN PRODUCTION IN TREATED PLANTS

[0163] Wheat plants were harvested at day 15 or 16 post-seed planting. The harvested wheat from each experimental group was measured for leaf length / pl ant height, wet weight, and nutrient uptake and protein accumulation / uptake.

[0164] Figure 12 and Table 13 depict the observed enhanced plant height in the two experimental groups (groups 2 and 3) relative to the control group.

[0165] Table 13: Pre-harvested Day 15 Wheat Leaf Length (N=5)Plant Number Leaf Height (cm)- Leaf Height (cm)- Leaf Height (cm)- Group 1 Group 2 Group 31 (Wi) 20 25.50 29.002 (Wii) 24.50 26.00 29.403 (Wiii) 24.30 29.10 22.704 (Wiv) 19.90 25.50 28.00Atty Docket LZ0003PCT| 5 (Wv) | 23.00| 28.10| 30.60

[0166] Figure 13 and Table 14 depict the observed enhanced plant wet weight m the two experimental groups (groups 2 and 3) relative to the control group. Wheat treated with product showed at least about a 25% to 55% increase in wet mass relative to untreated wheat.

[0167] Table 14: Pre-harvested Day 15 Wheat Wet Mass in Grams (N=5)Plant Number Mass (g) - Group 1 Mass (g) - Group 2 Mass (g) - Group 3 1 (Wi) 1.4 2.4 2.92 (Wii) 2.2 2.1 2.83 (Wiii) 1.6 2.2 2.34 (Wiv) 1.5 2.2 2.55 (Wv) 2.0 2.3 2.7Average 1.74 2.24 2.64

[0168] Wheat shoots / leaves were collected / harvested at day 15 or 16 post-seeding. All five samples from each experimental group were pooled and were tested for nitrogen (N), phosphorus (P), potassium (K), sulfur (S), zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), calcium (Ca), magnesium (Mg), and total protein content. The results are presented in Table 15, which shows a greater than 40% increase in leaf protein content in wheat treated with product (groups 2 and 3) relative to the untreated wheat group (group 1), and an increase in nitrogen of greater than 40%, an increase in phosphorous of greater than 150%, an increase in iron or greater than 10%, and an increase in zinc of greater than 50% relative to no-product control. Similar results were obtained for bean and zucchini (data not shown).

[0169] Table 15; Wheat leaf nutrient contentSample Group 1W (control) 2W (soil prepped 3W (soil prepped with product) with product +weekly product application)N % 3.81 5.61 5.65Protein % 23.80 35.04 35.32P % 0.730 2.107 2.216K% 5.68 5.87 5.74Ca% 0.37 0.36 0.37Mg % 0.331 0.304 0.304S04-S % 0.676 0.604 0.576Cu ppm 8.2 7.4 6.6Atty Docket LZ0003PCTFe ppm 88.2 97.7 105.1Mn ppm 125.2 89.9 87Zn ppm 25.2 39.9 41.8

Claims

Atty Docket LZ0003PCTCLAIMS1. A liquid agricultural input composition comprising a phytohormone, a secondary metabolite, an effective microbe, a plant macronutrient, a plant micronutrient, a biogenic amine, an enzyme, a sugar, a sugar alcohol, an amino acid, and water.2 The composition of claim 1 comprising at least two phytohormones, at least two secondary metabolites, at least two effective microbes, at least two plant macronutrients, at least two biogenic amines, at least two enzymes, at least two sugars, at least two sugar alcohols, and at least two amino acids.

3. The composition of claim 1 that does not comprise a suspended solid greater than 100 microns in diameter.

4. The composition of claim 1 comprising 6% - 10% solids.

5. The composition of claim 1 comprising 2% - 6% total carbon.

6. The composition of claim 1 having a pH of 3 – 6.

7. The composition of claim 1, wherein the phytohormone is selected from the group consisting of a gibberellin or derivative thereof, an auxin or derivative thereof, a cytokinin or derivative thereof, and an abscisic acid or derivative thereof.

8. The composition of claim 1, wherein the secondary metabolite is selected from the group consisting of lactic acid, succinic acid, shikimic acid, 2,3-butanediol, tricarballylic acid, and 3-deoxyhexonic acid.

9. The composition of claim 1, wherein the effective microbe is selected from the group consisting of Lactobacillus sp., Oceanobacillus sp., Bacillus sp., Myroides sp., Terribacillus sp.. Aneurinibacillus sp.. Paenibacillus sp., and Lysinibacillus sp.

10. The composition of claim 1, wherein the plant macronutrient is selected from the group consisting of nitrogen, phosphorous, potassium, calcium, magnesium, and sulfur. The composition of claim 1, wherein the plant micronutrient is selected from the group consisting of zinc, chlorine, boron, molybdenum, iron, and manganese.

12. The composition of claim 1, wherein die biogenic amine is selected from the group consisting of cadaverine, putrescine, tyramine, and ethanolamine.Atty Docket LZ0003PCT13. The composition of claim 1, wherein the enzyme is selected from the group consisting of phosphatase, cellulase, and nitrogenase.

14. The composition of claim 1, wherein the sugar is selected from the group consisting of trehalose, tagatose, fructose, sucrose, glucose, glucopyranose, mannose, maltose, xylose, lactose, galactose, and kestose.

15. The composition of claim 1, wherein the sugar is selected from the group consisting of erythritol, inositol, glycerol, mannitol, ribitol, sorbitol, and adonitol.

16. The composition of claim 1, wherein the amino acid is selected from the group consisting of pyroglutamic acid, gamma amino butyric acid, valine, aspartic acid, 5-aminovaleric acid, and serine.

17. The composition of claim 1 comprising 1 ng / g - 400 ng / g of abscisic acid or metabolites thereof.

18. The composition of claim 1 comprising 1 ng / g - 20 ng / g of abscisic acid.

19. The composition of claim 1 comprising 0.5 ng / g - 40 ng / g of cytokinin.

20. The composition of claim 1 comprising 20 ng / g - 80 ng / g of indole acetic acid (IAA).

21. The composition of claim 1 comprising 0.5 ng / g - 25 ng / g of gibberellin22. The composition of claim 1 comprising lactic acid and 2.3-butanediol.

23. The composition of claim 1 comprising 3-deoxyhexonic acid and succinic acid.

24. The composition of claim 1 comprising tricarballylic acid and shikimic acid.

25. The composition of claim 1 comprising Lactobacillus gallinarum and Lactobacillus johnsonii.

26. The composition of claim 1 comprising Bacillus subtilis and Paenibacillus polymyxa.

27. The composition of claim 1 comprising no more than 2% total Kjeldahl nitrogen.

28. The composition of claim 1 comprising 0.05% - 0.5% total Kjeldahl nitrogen.

29. The composition of claim 1 comprising no more than 2% phosphate.

30. The composition of claim 1 comprising 0.1% - 0.5% phosphate,31. The composition of claim 1 comprising no more than 2% potash.Atty Docket LZ0003PCT32. The composition of claim 1 comprising 0.1% - 1% potash.

33. The composition of claim 1 comprising no more than 1% sulfur.

34. The composition of claim 1 comprising 0.05% - 0.2% sulfur.

35. The composition of claim 1 comprising no more than 1% calcium.

36. The composition of claim 1 comprising 0.1% - 0.6% calcium.

37. The composition of claim 1 comprising no more than 1% magnesium.

38. The composition of claim 1 comprising 0.03% - 0.09% magnesium.

39. The composition of claim 1 comprising no more than 500 ppm iron.

40. The composition of claim 1 comprising 50 ppm - 300 ppm iron.

41. The composition of claim 1 comprising no more than 100 mg / kg zinc.

42. The composition of claim 1 comprising 1 mg / kg – 10 mg / kg zinc.

43. The composition of claim 1 comprising at least 10,000 mg / L of total organic carbon.

44. The composition of claim 1 comprising 15,000 mg / L - 40,000 mg / L of total organic carbon.

45. The composition of claim 1 having a carbon to nitrogen ratio (C: N) of at least 2:

1.

46. The composition of claim 1 having a C: N of 5:1 - 25: 1.

47. The composition of claim 1 comprising cadaverine and putrescine.

48. The composition of claim 1 comprising phosphatase and nitrogenase.

49. The composition of claim 1 comprising fructose, trehalose, and sucrose.

50. The composition of claim 1 comprising mannitol, inositol, and glycerol.

51. The composition of claim 1 comprising pyroglutamic acid and gamma aminobutyric acid (GABA).

52. The composition of claim 1, wherein said composition is a plant biostimulant.

53. The composition of claim 1, wherein said composition is a soil conditioner or amendment.Atty Docket LZ0003PCT54. The composition of claim 1, wherein said composition is a biopesticide.

55. The composition of claim 1, wherein said composition is a bioadj uvant.

56. A method of making a liquid agricultural input of any one of claims 1-55, the method comprising (a) in a vessel, combining an animal byproduct with a plant byproduct in water to form a first suspension, (b) pasteurizing the first suspension to form a pasteurized suspension, (c) adding gram-positive bacteria to the pasteurized suspension to form a bioreaction suspension, (d) incubating the bioreaction suspension to form a fermentation product suspension, and (e) removing solid material from the fermentation product suspension to form a liquid agricultural input.

57. The method of claim 56 further comprising sparging air into the vessel after the pasteurizing and during the incubating.

58. The method of claim 56 further comprising mixing the first suspension, the pasteurized suspension, the bioreaction suspension, and the fermentation product suspension.

59. The method of claim 56, wherein said mixing is continuous.

60. The method of claim 56. wherein said mixing is discontinuous.

61. The method of claim 56. wherein the pasteurizing is at > 55°C for > 24 hours.

62. The method of claim 56, wherein the gram-positive bacteria having an OD600 of 0.5 – 1.8 or having a titer of 1E6 cfu / mL – 3E8 cfu / mL is added to the pasteurized suspension at a dilution of 1: 10 - 1;500.

63. The method of claim 56, wherein the incubating is at 30°C - 50°C for 24 hours - 96 hours.

64. The method of claim 56, wherein the solid material is removed by passing the suspension over a 100 mesh – 300 mesh screen and retaining the liquid filtrate.

65. The method of claim 56, wherein the solid material is removed by centrifuging the suspension and retaining the liquid supernatant.

66. The method of claim 56, wherein the plant byproduct is molasses.

67. The method of claim 56, wherein the plant byproduct is at 5%-15% by weight in the first suspension.Atty Docket LZ0003PCT68. The method of claim 56, wherein the plant byproduct is at 10% by weight in the first suspension.

69. The method of claim 56. wherein the animal byproduct is fish meal.

70. The method of claim 56. wherein the animal byproduct is at 1 %- 10% by weight m the first suspension.

71. The method of claim 56. wherein the animal byproduct is at 5% by weight in the first suspension.

72. The method of claim 56, wherein the gram-positive bacteria is a facultative anerobic firmi cutes.

73. The method of claim 56, wherein the gram-positive bacteria is Paenibacillus polymyxa.

74. A system for producing a liquid agricultural input of any one of claims 1-55, the system comprising a vessel having a top, a bottom, and sides enclosing an inner cavity' from an outside environment, a mixer, an air pump, and a temperature regulator, configured to hold a suspension comprising solid material suspended in liquid material within the inner cavity'.

75. The system of claim 74. wherein the vessel is a tank or drum.

76. The system of claim 74, w'herein the vessel is a drum.

77. The system of claim 74, w'herein the vessel is a polymer tank having a capacity' of at least 450 gallons.

78. The system of claim 74, wherein the mixer is a submersible pump situated in the inner cavity and at the bottom of the vessel and configured to move the suspension from the bottom to the top of the vessel within the cavity' of the vessel.

79. The system of claim 74, wherein the air pump is a regenerative blow'er situated outside of the vessel and configured to inject air through a pipe into the inner cavity proximate the bottom of the vessel.

80. The system of claim 74, wherein the temperature regulator is a heater.

81. The system of claim 74, wherein the temperature regulator is a thermostatically controlled electric blanket configured to wrap around the outside of the vessel.Atty Docket LZ0003PCT82. The system of claim 74, wherein the temperature regulator is an IBC tote warming blanket wrapped around the outside of the vessel.

83. The system of claim 74 further comprising a liquid separator configured to receive the suspension from the vessel and to separate the solid material from the liquid material.

84. The system of claim 74, wherein the separator is a vibration separator.

85. The system of claim 74, wherein the separator is a vibration separator comprising a 180 mesh ~ 240 mesh sieve.

86. A method for conditioning soil for use in agriculture, horticulture, or potted plants comprising applying to the soil a liquid agricultural input composition of any one of claims 1-55.

87. The method of claim 86, wherein the product is diluted 1 part product to from 3 parts diluent to 100 parts diluent (1:3 - 1:100, product: diluent).

88. The method of claim 86, wherein the product is diluted 1:3 in water or an aqueous solution and applied to the soil of a potted plant.

89. The method of claim 86, wherein the product in diluted form is applied to a garden plot at a rate of about 1 gallon per acre to about 1 quart per 1,000 square feet.

90. The method of claim 86, wherein the product m diluted form is applied to a field at a rate of about 1 gallon to 3 gallons per acre.

91. The method of claim 86, wherein the product in diluted form is applied to a field at a rate of about 2 gallons per acre.

92. The method of claim 86, wherein the humidity or water holding capacity of the soil is improved.

93. The method of claim 86, wherein the soil moisture is increased by at least 40% relative to soil without product.

94. The method of claim 86, wherein the pH of the soil is less than 7.5.

95. The method of claim 86, wherein the soil pH is decreased by at least 5% relative to soil without product.Atty Docket LZ0003PCT96. The method of claim 86, wherein plants grown in the conditioned soil grow by height at a rate that is at least 8% greater than the growth rate of similar plants grown without application of the product to the soil.

97. The method of claim 86, wherein plants grown in the conditioned soil grow by wet shoot mass at a rate that is at least 20% greater than the growth rate of similar plants grown without application of the product to the soil.

98. The method of claim 86, wherein plants grown in the conditioned soil produce at least 40% more protein than plants grown without application of the product to the soil.

99. The method of claim 86, wherein plants grown in the conditioned soil accumulate at least 150% more phosphorous than plants grown without application of the product to the soil.

100. The method of claim 86, wherein plants grown in the conditioned soil accumulate at least 40% more nitrogen than plants grown without application of the product to the soil.

101. The method of claim 86, wherein plants grown in the conditioned soil accumulate at least 10% more iron than plants grown without application of the product to the soil.102 The method of claim 86. wherein plants grown in the conditioned soil accumulate at least 50% more zinc than plants grown without application of the product to the soil.

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