A biochemical reaction device and a biochemical testing system

Abstract

A biochemical reaction device and a biochemical test system relate to the field of biochemical test technology, and include a first shell, a second shell and a clamping assembly. The first shell has a accommodating chamber, and a partition assembly is arranged in the accommodating chamber to divide the accommodating chamber into a first sub-chamber and a second sub-chamber. The first sub-chamber is used to store samples. The second shell is accommodated in the second sub-chamber. The second shell has a accommodating chamber, and the accommodating chamber is used to store reagents. A transport assembly is arranged on the second shell, one end of the transport assembly extends into the second shell and communicates with the accommodating chamber, and the other end of the transport assembly extends toward a direction close to the partition assembly; one end of the clamping assembly extends into the second sub-chamber and abuts against the second shell, and pressing the other end of the clamping assembly can push the second shell to move relative to the first shell, so as to drive the other end of the transport assembly to pass through the partition assembly and communicate with the first sub-chamber, so that the sample in the first sub-chamber enters the accommodating chamber through the transport assembly and reacts with the reagent in the accommodating chamber.

Description

Technical Field

[0001] The present invention relates to the technical field of biochemical testing, and in particular to a biochemical reaction device and a biochemical testing system. Background Art

[0002] Current biochemical test equipment, such as blood coagulation test equipment and platelet function test equipment, generally uses separate sample cups, reagent cups and reaction cups. Among them, the sample and the reagent are stored in the sample cup and the reagent cup respectively. When performing a biochemical test, the sample and the reagent are respectively sucked by a sampling device and moved to the reaction cup, where they react to perform the biochemical test.

[0003] The above process requires a complex sampling system, motion control and sampling accuracy control, and has problems such as cumbersome operation, high equipment cost, and long test process time. In addition, for different samples sharing a sampling system, even if the sampling needle is cleaned by flushing the system, cross contamination may occur due to incomplete flushing, affecting the accuracy, reliability and consistency of the test. Summary of the invention

[0004] The object of the present invention is to provide a biochemical reaction device and a biochemical testing system, which have the advantages of simple structure and easy operation, can avoid cross contamination, and improve the accuracy, reliability and consistency of the test.

[0005] The embodiment of the present invention is achieved as follows:

[0006] In one aspect of an embodiment of the present invention, a biochemical reaction device is provided, comprising a first shell, a second shell and a pressing assembly, wherein the first shell has a receiving chamber, the receiving chamber is provided with a partition assembly to divide the receiving chamber into a first sub-chamber and a second sub-chamber, the first sub-chamber is used to store samples, the second shell is contained in the second sub-chamber, the second shell has a receiving chamber, the receiving chamber is used to store reagents, a transport assembly is provided on the second shell, one end of the transport assembly extends into the second shell and communicates with the receiving chamber, and the other end of the transport assembly extends toward the direction close to the partition assembly; one end of the pressing assembly extends into the second sub-chamber and abuts against the second shell, and pressing the other end of the pressing assembly can push the second shell to move relative to the first shell in the second sub-chamber, so as to drive the other end of the transport assembly to pass through the partition assembly and communicate with the first sub-chamber, so that the sample in the first sub-chamber enters the receiving chamber through the transport assembly and reacts with the reagent in the receiving chamber. The biochemical reaction device has the advantages of simple structure and easy operation, can avoid cross contamination, and improve the accuracy, reliability and consistency of the test.

[0007] Optionally, the clamping assembly includes a pressure head, an opening is provided on the second shell, the pressure head abuts against the second shell so that the pressure head seals the opening, an air intake channel is provided in the pressure head, one end of the air intake channel is used to communicate with the air valve, and the other end of the air intake channel is used to communicate with the accommodating chamber.

[0008] Optionally, the clamping assembly further includes a sealing ring, a protrusion extends from one end of the pressure head close to the second shell, the sealing ring is sleeved on the protrusion, the protrusion extends into the second shell through the opening, and the sealing ring abuts against the second shell.

[0009] Optionally, the partition assembly includes a partition, a guide plate and a diaphragm connected in sequence, the side wall surface of the first shell is arranged parallel to the partition, the bottom surface of the first shell and the guide plate are arranged inclined respectively, and the other end of the transport assembly is against the diaphragm.

[0010] Optionally, the transport component includes a capillary channel and a puncture needle, the capillary channel is arranged on the second shell, one end of the capillary channel extends into the second shell and communicates with the accommodating cavity, the other end of the capillary channel is connected to the needle tail of the puncture needle, and the needle tip of the puncture needle is against the diaphragm.

[0011] Optionally, a carrier frame is provided in the accommodating cavity, a carrier film is provided on the carrier frame, the carrier film is used to carry reagents, and one end of the capillary channel passes through the carrier frame and is connected to the carrier film.

[0012] Optionally, the diaphragm is a porous biological membrane, and the supporting membrane is a nitrocellulose membrane.

[0013] Optionally, at least one side rib is provided on the inner wall surface of the second sub-chamber, and the side wall surface of the side rib facing the center of the second sub-chamber is in contact with the outer wall surface of the second shell.

[0014] Optionally, a cover body is further included, and the cover body is used to cover the first shell.

[0015] Another aspect of the present invention provides a biochemical test system, comprising the above-mentioned biochemical reaction device. The biochemical reaction device has the advantages of simple structure and easy operation, can avoid cross contamination, and improve the accuracy, reliability and consistency of the test.

[0016] The beneficial effects of the embodiments of the present invention include:

[0017] The biochemical reaction device includes a first shell, a second shell and a pressing assembly. The first shell has a accommodating chamber, and a partition assembly is arranged in the accommodating chamber to divide the accommodating chamber into a first sub-chamber and a second sub-chamber. The first sub-chamber is used to store samples. The second shell is accommodated in the second sub-chamber. The second shell has an accommodating chamber, and the accommodating chamber is used to store reagents. Since the accommodating chamber and the first sub-chamber can be relatively independent of each other, the biochemical reaction device can avoid external contamination of samples and reagents during the storage stage before the biochemical reaction test is started. A transport assembly is arranged on the second shell, one end of the transport assembly extends into the second shell and communicates with the accommodating chamber, and the other end of the transport assembly extends in a direction close to the partition assembly, so that the second shell can drive the transport assembly to move in a direction close to or away from the partition assembly. One end of the clamping assembly extends into the second sub-chamber and abuts against the second shell. Pressing the other end of the clamping assembly can push the second shell to move relative to the first shell in the second sub-chamber, so as to drive the other end of the transport assembly to pass through the partition assembly and communicate with the first sub-chamber, so that the sample in the first sub-chamber enters the accommodating chamber through the transport assembly and reacts with the reagent in the accommodating chamber. In the above process, the accommodating chamber of the second shell also serves to provide a reaction place for the biochemical reaction test between the sample and the reagent, so as to avoid external contamination of the sample and the actual during the biochemical reaction test. In summary, the biochemical reaction device has the advantages of simple structure and easy operation. At the same time, for the test of different samples, different biochemical reaction devices can be used for testing, so that the biochemical reaction device can avoid cross contamination and improve the accuracy, reliability and consistency of the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 One of the structural schematic diagrams of the biochemical reaction device provided in an embodiment of the present invention;

[0020] Figure 2 A second structural schematic diagram of a biochemical reaction device provided in an embodiment of the present invention;

[0021] Figure 3 A third structural schematic diagram of a biochemical reaction device provided in an embodiment of the present invention;

[0022] Figure 4 A fourth structural schematic diagram of a biochemical reaction device provided in an embodiment of the present invention;

[0023] Figure 5 The fifth structural schematic diagram of the biochemical reaction device provided in the embodiment of the present invention.

[0024] Icons: 100-biochemical reaction device; 10-first shell; 11-first sub-chamber; 12-second sub-chamber; 121-side ribs; 20-second shell; 21-carrying frame; 22-carrying film; 30-pressing assembly; 31-pressing head; 311-air inlet channel; 312-protrusion; 32-sealing ring; 40-partitioning assembly; 41-partition plate; 42-guide plate; 43-diaphragm; 50-transportation assembly; 51-capillary tube; 52-puncture needle; 60-cover body. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0028] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0029] In addition, the terms "horizontal", "vertical" and the like do not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0030] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be a connection between two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] Please refer to Figures 1 to 3 The present embodiment provides a biochemical reaction device 100, comprising a first shell 10, a second shell 20 and a pressing assembly 30. The first shell 10 has a receiving chamber, and a partition assembly 40 is arranged in the receiving chamber to divide the receiving chamber into a first sub-chamber 11 and a second sub-chamber 12. The first sub-chamber 11 is used to store samples. The second shell 20 is accommodated in the second sub-chamber 12. The second shell 20 has a receiving chamber, and the receiving chamber is used to store reagents. The second shell 20 is provided with a transport assembly 50, and one end of the transport assembly 50 extends into the second sub-chamber 12. The housing 20 is connected to the accommodating chamber, and the other end of the transport component 50 extends toward the direction close to the partition component 40; one end of the pressing component 30 extends into the second sub-chamber 12 and abuts against the second housing 20, and pressing the other end of the pressing component 30 can push the second housing 20 to move relative to the first housing 10 in the second sub-chamber 12, so as to drive the other end of the transport component 50 to pass through the partition component 40 and communicate with the first sub-chamber 11, so that the sample in the first sub-chamber 11 enters the accommodating chamber through the transport component 50 and reacts with the reagent in the accommodating chamber. The biochemical reaction device 100 has the advantages of simple structure and easy operation, can avoid cross contamination, and improve the accuracy, reliability and consistency of the test.

[0032] It should be noted that the biochemical reaction device 100 includes a first shell 10, the first shell 10 has a accommodating chamber, and a partition component 40 is provided in the accommodating chamber to divide the accommodating chamber into a first sub-chamber 11 and a second sub-chamber 12. Since the partition component 40 is provided in the accommodating chamber, the first sub-chamber 11 and the second sub-chamber 12 formed after the accommodating chamber is divided can be relatively independent of each other. In this way, the biochemical reaction device 100 can store samples in the first sub-chamber 11 to ensure that the samples are not contaminated during the storage stage before the biochemical reaction test begins.

[0033] The biochemical reaction device 100 further includes a second housing 20, which is accommodated in the second sub-chamber 12, and has a receiving cavity in the second housing 20. The receiving cavity and the first sub-chamber 11 can be relatively independent of each other, so that the biochemical reaction device 100 can store reagents in the receiving cavity to ensure that the reagents are not contaminated during the storage stage before the biochemical reaction test begins.

[0034] In addition, a transport component 50 is also provided on the second shell 20, wherein one end of the transport component 50 extends into the second shell 20 and communicates with the accommodating cavity, and the other end of the transport component 50 extends toward the direction close to the partition component 40. In this way, when the second shell 20 moves in the second sub-chamber 12 relative to the first shell 10 toward the direction close to the partition component 40, the other end of the transport component 50 can move toward the direction close to the partition component 40; when the second shell 20 moves in the second sub-chamber 12 relative to the first shell 10 toward the direction away from the partition component 40, the other end of the transport component 50 can move toward the direction away from the partition component 40.

[0035] In order to facilitate driving the second shell 20 to move relative to the first shell 10 in the second sub-chamber 12, the biochemical reaction device 100 also includes a clamping component 30, one end of which extends into the second sub-chamber 12 and abuts against the second shell 20. In this way, the operator can press the other end of the clamping component 30 to force one end of the clamping component 30 to push the second shell 20 to move relative to the first shell 10, thereby driving the other end of the transport component 50 to move toward the direction close to the partition component 40 until it passes through the partition component 40 and is connected to the first sub-chamber 11, so that the sample in the first sub-chamber 11 can be transported to one end of the transport component 50 through the other end of the transport component 50, until it enters the accommodating chamber through the transport component 50, and finally reacts with the reagent in the accommodating chamber.

[0036] The biochemical reaction device 100 provided by the present application stores samples separately through the first sub-chamber 11 of the first shell 10, and stores reagents separately through the accommodating chamber of the second shell 20, so as to prevent the samples and reagents from being contaminated by the outside during the storage stage before the biochemical reaction test is started. The second shell 20 is pushed to move relative to the first shell 10 in the second sub-chamber 12 by pressing the clamping component 30, so that the sample stored in the first sub-chamber 11 can enter the accommodating chamber through the transport component 50, and perform a biochemical reaction test with the reagent stored in the accommodating chamber. In the above process, the accommodating chamber of the second shell 20 also plays the role of providing a reaction place for the biochemical reaction test between the sample and the reagent, so as to prevent the sample and the reagent from being contaminated by the outside during the biochemical reaction test.

[0037] As described above, the biochemical reaction device 100 includes a first shell 10, a second shell 20 and a pressing assembly 30. The first shell 10 has a accommodating chamber, and a partition assembly 40 is arranged in the accommodating chamber to divide the accommodating chamber into a first sub-chamber 11 and a second sub-chamber 12. The first sub-chamber 11 is used to store samples, and the second shell 20 is accommodated in the second sub-chamber 12. The second shell 20 has an accommodating chamber, and the accommodating chamber is used to store reagents. Since the accommodating chamber and the first sub-chamber 11 can be relatively independent of each other, the biochemical reaction device 100 can avoid external contamination of samples and reagents during the storage stage before the biochemical reaction test is started. A transport assembly 50 is arranged on the second shell 20, one end of the transport assembly 50 extends into the second shell 20 and communicates with the accommodating chamber, and the other end of the transport assembly 50 extends in a direction close to the partition assembly 40, so that the second shell 20 can drive the transport assembly 50 to move in a direction close to or away from the partition assembly 40. One end of the clamping assembly 30 extends into the second sub-chamber 12 and abuts against the second shell 20. Pressing the other end of the clamping assembly 30 can push the second shell 20 to move relative to the first shell 10 in the second sub-chamber 12, so as to drive the other end of the transport assembly 50 to pass through the partition assembly 40 and communicate with the first sub-chamber 11, so that the sample in the first sub-chamber 11 enters the accommodating chamber through the transport assembly 50 and reacts with the reagent in the accommodating chamber. In the above process, the accommodating chamber of the second shell 20 also plays a role in providing a reaction place for the biochemical reaction test between the sample and the reagent, so as to avoid the sample and the actual being contaminated by the outside during the biochemical reaction test. In summary, the biochemical reaction device 100 has the advantages of simple structure and easy operation. At the same time, for the test of different samples, different biochemical reaction devices 100 can be used for testing, so that the biochemical reaction device 100 can avoid cross contamination and improve the accuracy, reliability and consistency of the test.

[0038] like Figures 1 to 3 As shown, in order to allow the sample in the first sub-chamber 11 to smoothly pass through the transport assembly 50 and enter the accommodating chamber, in this embodiment, the pressing assembly 30 includes a pressure head 31, an opening is provided on the second shell 20, the pressure head 31 abuts against the second shell 20 to seal the opening, and an air inlet channel 311 is provided in the pressure head 31, one end of the air inlet channel 311 is used to communicate with the air valve, and the other end of the air inlet channel 311 is used to communicate with the accommodating chamber. In this way, when the operator connects one end of the air inlet channel 311 to the air valve of the external environment, the gas in the air inlet channel 311 can be firstly extracted through the suction effect of the air valve, and then the sample in the first sub-chamber 11 can be transported to the accommodating chamber through the transport assembly 50.

[0039] like Figures 1 to 3As shown, in order to better seal the opening on the second housing 20, in this embodiment, the pressing assembly 30 further includes a sealing ring 32, and a protrusion 312 extends from one end of the pressure head 31 close to the second housing 20. The sealing ring 32 is sleeved on the protrusion 312, and the protrusion 312 extends into the second housing 20 through the opening, and the sealing ring 32 abuts against the second housing 20. The material of the sealing ring 32 can be rubber, silicone, etc., which is not specifically limited here, and those skilled in the art should be able to make reasonable selections and designs according to actual conditions.

[0040] like Figure 2 and Figure 3 As shown, in this embodiment, the partition component 40 includes a partition plate 41, a guide plate 42 and a diaphragm 43 connected in sequence, the side wall surface of the first housing 10 is arranged parallel to the partition plate 41, and the bottom surface of the first housing 10 and the guide plate 42 are arranged obliquely, so that the sample can be stored as much as possible on the side close to the diaphragm 43, so that it is convenient for the transport component 50 to transport the sample to the accommodating cavity, and the other end of the transport component 50 abuts against the diaphragm 43, so that the other end of the transport component 50 can easily pass through the diaphragm 43 and communicate with the first sub-chamber 11. Furthermore, the diaphragm 43 is provided with a hole for the transport component 50 to pass through.

[0041] like Figure 2 and Figure 3 As shown, in this embodiment, the transport assembly 50 includes a capillary tube 51 and a puncture needle 52. The capillary tube 51 is disposed on the second housing 20. One end of the capillary tube 51 extends into the second housing 20 and communicates with the accommodating cavity. The other end of the capillary tube 51 is connected to the needle tail of the puncture needle 52. The needle tip of the puncture needle 52 abuts against the diaphragm 43. The capillary tube 51 can facilitate the transport of the sample in the first sub-chamber 11 to the accommodating cavity, and the puncture needle 52 can facilitate the penetration of the diaphragm 43 and communicate with the first sub-chamber 11.

[0042] like Figures 1 to 3 As shown, in this embodiment, a carrier 21 is provided in the accommodating cavity, and a carrier film 22 is provided on the carrier 21. The carrier film 22 is used to carry the reagent, and one end of the capillary tube 51 passes through the carrier 21 and is connected to the carrier film 22. Optionally, the diaphragm 43 is a porous biological membrane, and the carrier film 22 is a nitrocellulose membrane. Regarding the specific structure of the carrier 21, those skilled in the art should be able to make reasonable selections and designs according to actual conditions, and no specific restrictions are made here. In addition, when there are multiple reagents, a partition 41 can also be extended from the carrier 21 toward the center of the carrier film 22 so that multiple reagents can be separated and stored separately.

[0043] like Figure 2 and Figure 3As shown, in this embodiment, at least one side rib 121 is provided on the inner wall surface of the second sub-chamber 12, and the side wall surface of the side rib 121 facing the center of the second sub-chamber 12 is in contact with the outer wall surface of the second shell 20. Preferably, the side wall surface of the side rib 121 facing the center of the second sub-chamber 12 can be inclined.

[0044] In this way, when the second shell 20 is accommodated in the second sub-chamber 12, the side wall surface of the side rib 121 facing the center of the second sub-chamber 12 fits with the outer wall surface of the second shell 20, so that the second shell 20 and the first shell 10 can remain relatively stable in the absence of external force, so that before the biochemical reaction test begins, the transport component 50 can not pass through the partition component 40, thereby ensuring that the state of relative independence between the sample and the reagent is broken. Under the pushing action of the clamping component 30, the second shell 20 and the first shell 10 can move relative to each other, so that during the biochemical reaction test, the transport component 50 can pass through the partition component 40, thereby breaking the state of relative independence between the sample and the reagent.

[0045] In addition, regarding the number of side ribs 121, those skilled in the art should be able to make reasonable selections and designs according to actual conditions, and no specific restrictions are imposed here. For example, the number of side ribs 121 is one, two, three, or four. When the number of side ribs 121 is multiple, the multiple side ribs 121 can be evenly distributed along the circumference of the first sub-chamber 11, so that the side wall surfaces of the multiple side ribs 121 facing the center of the second sub-chamber 12 can provide uniform friction for the outer wall surface of the second shell 20.

[0046] Please refer to Figure 4 and Figure 5 In this embodiment, the biochemical reaction device 100 further includes a cover 60, which is used to cover the first shell 10, so that the biochemical reaction device 100 can ensure that the first sub-chamber 11, the second sub-chamber 12 and the accommodating chamber are clean and free of pollution before use. Preferably, the opening of the second shell 20 can also be sealed by adding a separate sealing film.

[0047] The present application also provides a biochemical test system. The biochemical test system provided in this embodiment includes the above-mentioned biochemical reaction device 100. Since the structure and beneficial effects of the biochemical reaction device 100 have been described in detail in the above-mentioned embodiments, they will not be repeated here.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A biochemical reaction device, characterized in that: The invention comprises a first shell, a second shell and a pressing assembly, wherein the first shell has a receiving chamber, the receiving chamber is provided with a partition assembly to divide the receiving chamber into a first sub-chamber and a second sub-chamber, the first sub-chamber is used to store samples, the second shell is accommodated in the second sub-chamber, the second shell has a receiving chamber, the receiving chamber is used to store reagents, the second shell is provided with a transport assembly, one end of the transport assembly extends into the second shell and communicates with the receiving chamber, and the other end of the transport assembly extends toward the direction close to the partition assembly; One end of the pressing component extends into the second sub-chamber and abuts against the second shell, and pressing the other end of the pressing component can push the second shell to move relative to the first shell in the second sub-chamber, so as to drive the other end of the transport component to pass through the partition component and communicate with the first sub-chamber, so that the sample in the first sub-chamber enters the accommodating chamber through the transport component and reacts with the reagent in the accommodating chamber; The clamping assembly includes a pressure head, an opening is provided on the second shell, the pressure head abuts against the second shell so that the pressure head closes the opening, an air intake channel is provided in the pressure head, one end of the air intake channel is used to communicate with the air valve, and the other end of the air intake channel is used to communicate with the accommodating chamber.

2. The biochemical reaction device according to claim 1, characterized in that: The clamping assembly also includes a sealing ring. A protrusion extends from one end of the pressure head close to the second shell. The sealing ring is sleeved on the protrusion. The protrusion extends into the second shell through the opening, and the sealing ring abuts against the second shell.

3. The biochemical reaction device according to claim 1, characterized in that: The partition assembly includes a partition, a guide plate and a diaphragm connected in sequence, the side wall surface of the first shell is arranged in parallel with the partition, the bottom surface of the first shell and the guide plate are arranged in an inclined manner, and the other end of the transport assembly is against the diaphragm.

4. The biochemical reaction device according to claim 3, characterized in that: The transport component includes a capillary channel and a puncture needle. The capillary channel is arranged on the second shell. One end of the capillary channel extends into the second shell and communicates with the accommodating cavity. The other end of the capillary channel is connected to the needle tail of the puncture needle. The needle tip of the puncture needle is against the diaphragm.

5. The biochemical reaction device according to claim 4, characterized in that: A carrier is arranged in the accommodating cavity, a carrier film is arranged on the carrier, the carrier film is used to carry reagents, and one end of the capillary channel passes through the carrier and is communicated with the carrier film.

6. The biochemical reaction device according to claim 5, characterized in that: The diaphragm is a porous biological membrane, and the supporting membrane is a nitrocellulose membrane.

7. The biochemical reaction device according to claim 1, characterized in that: At least one side rib is disposed on the inner wall surface of the second sub-chamber, and the side wall surface of the side rib facing the center of the second sub-chamber is in contact with the outer wall surface of the second shell.

8. The biochemical reaction device according to claim 1, characterized in that: It also includes a cover body, which is used to cover the first shell.

9. A biochemical testing system, characterized in that: A biochemical reaction device comprising any one of claims 1-8.

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