Use of paclobutrazol as a synergist for insecticides

Abstract

The application discloses application of paclobutrazol as a synergist of insecticides, and belongs to the technical field of agricultural production. The paclobutrazol is mixed with chemical pesticides, so that plant growth can be regulated, the toxicity of the insecticides can be significantly improved, the pesticide effect duration of the insecticides can be prolonged, the control effect of the insecticides is improved, and the paclobutrazol has important values in delaying development of drug resistance and prolonging the life period of pesticide varieties. In addition, the paclobutrazol is suitable for being mixed with various insecticides.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of agricultural production, more particularly to the application of paclobutrazol as a synergist of insecticide. BACKGROUND

[0002] In agricultural production, the use of pesticides is one of the main measures for preventing and controlling pests of crops. However, long-term use of a single pesticide leads to the development of pesticide resistance in pests, which seriously affects the control effect of the pesticide and increases the loss of agricultural production. In order to maintain the control level, the amount of pesticide used or the number of times of pesticide use is increased, which in turn increases the cost of agricultural production. It is required in agricultural production to prevent or delay the development of pesticide resistance by using pesticides scientifically and reasonably, such as rotating different pesticides with different mechanisms of action, and scientifically mixing pesticides, so as to reduce the field use amount and the number of times of use of the pesticide. At the same time, it is also an effective means to enhance the insect resistance of plants or to reduce the ability of plants to degrade pesticides. The use of plant growth regulators can delay the development of pesticide resistance and improve the control effect of pesticides on pesticide-resistant pests.

[0003] Plant growth regulators are artificially synthesized (or extracted from natural microorganisms) organic compounds with similar growth and development regulation effects to natural plant hormones. The mechanism of action is to regulate the physiological activities of plants, adjust the morphological development of plants, change the enzyme activity of the antioxidant system of plants, and regulate the synthesis of insect-resistant substances, so as to change the insect resistance of plants and delay the metabolic speed of pesticides in plants, increase the duration of the existence of pesticides in plants, and thus improve the control effect. Plant growth regulators themselves have no physiological toxicity to pests, but when mixed with pesticides, they can achieve the purpose of prolonging the effective period of pesticide effect, improving the biological activity, reducing the amount of use, reducing the production cost and increasing the yield.

[0004] At present, plant growth regulators mainly include the following types: crop growth promoters (auxin analogs, gibberellins, cytokinins, brassinosteroids, etc.), plant growth retardants (paclobutrazol, uniconazole, etc.), and plant growth inhibitors (abscisic acid, green fresh, etc.).

[0005] The present application reports a plant growth regulator, paclobutrazol, which is used for the synergism of chemical pesticides. It has synergistic effect on various insecticides, and its chemical name is ((2RS, 3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1, 2, 4-triazole-1-yl) pentan-3-ol, and its English name is paclobutrazol. The chemical structure is as follows:

[0006]

[0007] The applicant finds through experimental research that the good synergistic effect on insect resistance is achieved by mixing paclobutrazol with nitenpyram, dinotefuran, triflumintrazole, pymetrozine and chlorantraniliprole in certain proportions, and the insecticidal rate is significantly improved. The mechanism of the synergistic effect of paclobutrazol on insect resistance is not clear at present, which may be that the enzyme activity of the rice antioxidant system is inhibited, the metabolism speed of the pesticide in the rice plant body is delayed, or the thickness of the rice stem sheath is increased to increase the difficulty of the pests to feed. SUMMARY

[0008] Therefore, the application aims to provide the application of paclobutrazol as a synergist of insecticides, and the main content is that paclobutrazol has a synergistic effect on nitenpyram, dinotefuran, triflumintrazole, pymetrozine and chlorantraniliprole, and the toxicity of the insecticides can be improved by mixing paclobutrazol with the insecticides, and paclobutrazol can be used in combination with nitenpyram, dinotefuran, triflumintrazole, pymetrozine and chlorantraniliprole.

[0009] To achieve the above-mentioned purpose, the application adopts the following technical solutions.

[0010] The application claims the application of paclobutrazol as a synergist of insecticides.

[0011] The application has the advantages that paclobutrazol can be used in combination with chemical pesticides to regulate plant growth, significantly improve the toxicity of insecticides, prolong the duration of the efficacy of insecticides, improve the control effect of insecticides, delay the development of insect resistance, and prolong the life of pesticide varieties, which has important value.

[0012] Further, the insecticides are nitenpyram, dinotefuran, triflumintrazole, pymetrozine or chlorantraniliprole.

[0013] Further, the synergistic weight ratio of paclobutrazol to nitenpyram is (1:10)-(3:1).

[0014] The application has the advantages that nitenpyram is an organic compound with the molecular formula of C 11 H 15 ClN4O2, the molecular weight is 270.715, the relative density is 1.255, the melting point / solidification point is 82℃, and the chemical structure is as follows:

[0015] It has excellent systemicity, penetration, broad insecticidal spectrum, safety and no drug harm, and can be widely applied to cucumber, eggplant, radish, tomato, grape, tea and rice to control various aphids, thrips, whiteflies and leafhoppers.

[0016] Further, the synergistic weight ratio of the above paclobutrazol and dinotefuran is (1:10)-(3:2).

[0017] The beneficial effect of the above further technical solution is that dinotefuran is an organic substance with a molecular formula of C7H 14 N4O3, a molecular weight of 202.211, a melting point of 107.℃, and a chemical structure of: It has a very wide insecticidal spectrum and is very safe to crops, livestock and the environment.

[0018] Further, the synergistic weight ratio of the above paclobutrazol and triflumezopyrim is (1:2)-(10:1).

[0019] The beneficial effect of the above further technical solution is that triflumezopyrim is an organic substance with a molecular formula of C 20 H 13 F3N4O2, a molecular weight of 398.338, and a chemical structure of:

[0020] It is mainly used for rice crop to control rice planthoppers.

[0021] Further, the synergistic weight ratio of the above paclobutrazol and pymetrozine is (3:20)-(2:1).

[0022] The beneficial effect of the above further technical solution is that pymetrozine has a molecular formula of C 10 H 11 N5O, a molecular weight of 217.227, colorless crystals, and a chemical structure of: It belongs to pyridine (pyridine imine) or triazinone insecticides, and is a non-killing insecticide. It was first developed by Ciba-Geigy in 1988. The product shows excellent control effect on various crop piercing-sucking pests. Pymetrozine has contact killing effect on pests, and also has systemic activity. It can be transported in xylem and phloem in plant body; therefore, it can be used as foliar spray and soil treatment. Due to its good transport characteristics, newly grown branches and leaves can also be effectively protected after stem and leaf spraying.

[0023] Further, the synergistic weight ratio of the above paclobutrazol and pymetrozine is (3:20)-(2:1).

[0024] The beneficial effects of the above further technical solutions are that the chlorantraniliprole has a molecular formula of C 18 H 14 BrCl2N5O2, a molecular weight of 483.15, and a pure product appearance of white crystal, and a chemical structure of:

[0025] The insecticide has high efficiency and wide spectrum, and has good control effect on noctuidae, tortricidae, pyralidae, tortricidae, gelechiidae, plutellidae, and other insects, and can also control various non- lepidopteran pests such as coleoptera curculionidae, chrysomelidae, diptera agromyzidae, and tobacco whitefly. The lepidopteran, main beetle and whitefly insecticide has reliable and stable control effect at a low dose, stops feeding immediately, has a longer drug effect period, is resistant to rain washing, and provides immediate and long-term protection at any stage of crop growth. The mechanism of action is different from other types of insecticides, can bind to the ryanodine receptor in the body of insects, inhibit insect feeding, cause insect contraction, and ultimately lead to insect death.

[0026] Further, the dosage form of the above-mentioned insecticide synergist is any one of water dispersible granules, wettable powder, suspension concentrate, oil suspension, emulsifiable concentrate, water emulsion, soluble liquid and seed coating agent.

[0027] According to the above technical solutions, compared with the prior art, the beneficial effects of the present application are as follows:

[0028] 1. The multi-effect azole has a synergistic effect on nitenpyram, dinotefuran, triflumezopyrim, pymetrozine and chlorantraniliprole, and mixing with nitenpyram, dinotefuran, triflumezopyrim, pymetrozine and chlorantraniliprole can improve the virulence, improve the control effect of nitenpyram, dinotefuran, triflumezopyrim, pymetrozine and chlorantraniliprole on resistant insects, and prolong the duration of nitenpyram, dinotefuran, triflumezopyrim, pymetrozine and chlorantraniliprole.

[0029] 2. The multi-effect azole is a kind of crop growth regulator, and the present application provides a new use of this kind of chemical agent, which can be applied to the field of pest control, and also provides a new idea for the innovation of pesticide combination. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the present application.

[0031] Embodiment 1

[0032] 1. Test agent

[0033] 15.0% paclobutrazol WP (Sichuan Runer Technology Co., Ltd., Guoguang brand); 30% nitenpyram WDG (Shaanxi Huarong Kaiwei Biological Co., Ltd.); 20% dinotefuran SC (Guangxi Guilin Hongtian Biochemical Co., Ltd.); 10% triflumuron SC (E. I. du Pont de Nemours and Company); 50% pymetrozine WDG (Jiangsu Anbang Electrical Co., Ltd.)

[0034] 2. Test insect source

[0035] The Nilaparvata lugens population collected in Fuyang, Hangzhou, Zhejiang in 2012 was artificially fed with TN1 rice plants in the laboratory under the conditions of temperature 27±1℃, relative humidity 80±10%, and photoperiod 16L:8D.

[0036] 3. Determination method

[0037] Nitenpyram, dinotefuran, triflumuron, and pymetrozine were respectively prepared into aqueous solutions according to the recommended field dosage (30 kg of water per mu of land), and different concentrations of paclobutrazol were respectively mixed with nitenpyram (weight ratio 3:1), dinotefuran (weight ratio 1:10), triflumuron (weight ratio 1:2), and pymetrozine (weight ratio 3:20) to prepare mixed solutions with the same insecticide concentration.

[0038] Pot-grown rice seedlings with the same growth vigor were selected, inverted and immersed for 30 s, and then placed upright in a transfer box (the rice seedlings were washed with clean water before immersion, and the number of tillers was trimmed to 7, with gaps between the tillers as much as possible, and then used after the surface was free of water droplets); the seedlings in the transfer box were placed in the laboratory for 1 h, with the leaves of the seedlings not touching each other, and then transferred to an artificial climate chamber after the leaf surface was free of liquid. The rice seedlings were randomly divided into 4 groups, and 3rd instar N. lugens nymphs with the same growth vigor were selected and introduced into the treated rice seedlings 14 days, 21 days, 28 days, and 35 days after treatment, with 15 nymphs per replicate; the number of dead N. lugens nymphs in each treatment was recorded 4 days later.

[0039] 4. Data processing

[0040] The significance of differences between treatments was calculated using SPSS 26.0 software, with a confidence interval of 95%.

[0041] Synergistic effect = (mortality rate of N. lugens nymphs in the complex treatment) / (mortality rate of N. lugens nymphs in the pesticide control).

[0042] 5. Experimental results

[0043] As shown in Tables 1-5.

[0044] Table 1 Synergistic effect of paclobutrazol on nitenpyram (weight ratio 3:1)

[0045]

[0046] From Table 1, it can be seen that the combination of paclobutrazol and nitenpyram can significantly improve the toxicity of nitenpyram to brown planthopper and prolong the efficacy of nitenpyram. The synergistic effect is 1.7 times at 14 days after treatment, 8.7 times at 21 days after treatment, 17 times at 28 days after treatment, and 14.9 times at 35 days after treatment.

[0047] Table 2 Synergistic effect of paclobutrazol on dinotefuran (weight ratio 1:10)

[0048]

[0049]

[0050] From Table 2, it can be seen that the combination of paclobutrazol and dinotefuran can significantly prolong the efficacy of dinotefuran. The synergistic effect is not significant at 14 days after treatment, 1.1 times at 21 days after treatment, 1.3 times at 28 days after treatment, and 1.8 times at 35 days after treatment.

[0051] Table 3 Synergistic effect of paclobutrazol on triflumezopyrim (weight ratio 1:2)

[0052]

[0053] From Table 3, it can be seen that the combination of paclobutrazol and triflumezopyrim can significantly prolong the efficacy of triflumezopyrim. The synergistic effect is not significant at 14 days after treatment, 1.8 times at 21 days after treatment, 2.5 times at 28 days after treatment, and 1.7 times at 35 days after treatment.

[0054] Table 4 Synergistic effect of paclobutrazol on pymetrozine (weight ratio 3:20)

[0055]

[0056] From Table 4, it can be seen that the combination of paclobutrazol and pymetrozine can significantly improve the toxicity of pymetrozine to brown planthopper and prolong the efficacy of pymetrozine. The synergistic effect is not significant at 14 days and 21 days after treatment, 1.1 times at 28 days after treatment, and 1.2 times at 35 days after treatment.

[0057] Table 5 Toxicity of different concentrations of paclobutrazol to third instar brown planthopper nymphs in the laboratory

[0058]

[0059] From Table 5, it can be seen that within the range of test concentrations, paclobutrazol has no lethal effect on third instar brown planthopper nymphs.

[0060] Example 2

[0061] 1. Test agents

[0062] 96% paclobutrazol TC (Beijing Solabio Technology Co., Ltd.), 2.63 g of the original drug was weighed, dissolved with acetone and made up to 100 mL to prepare a mother liquor of 25000 ppm for later use; 200 g / L chlorantraniliprole SC (U.S. FMC Corporation, trade name "Bonide").

[0063] 2. Field efficacy test and investigation method

[0064] Test site: rice field in Fuyang District, Hangzhou City, Zhejiang Province; rice variety: Zhongjiazhao 17, leaf spraying test was carried out on March 28, 2021 and April 23, a total of eight treatments were set up: ① 50 mg / L paclobutrazol; ② 150 mg / L paclobutrazol; ③ 300 mg / L paclobutrazol; ④ 50 mg / L paclobutrazol + 60 mg / L Bonide; ⑤ 150 mg / L paclobutrazol + 60 mg / L Bonide; ⑥ 300 mg / L paclobutrazol + 60 mg / L Bonide; ⑦ 60 mg / L Bonide. Each plot was 50 m 2 ; ⑧ blank treatment (without drug). Each treatment was repeated three times, and the Chilo suppressalis control effect investigation was carried out 2 weeks and 5 weeks after application, using diagonal 5-point sampling method, 5 points were investigated in each plot, 10 clusters of rice were investigated in each point, a total of 50 clusters of rice were investigated, the number of dead sheaths and dead hearts was investigated, and the dead sheath rate, dead heart rate and control effect were calculated.

[0065] 3. Control effect calculation

[0066] Chilo suppressalis dead sheath (heart) rate (%) = [dead sheath (heart) plant number / total plant number investigated] x 100;

[0067] Corrected control effect = (1 - damage rate of pesticide treatment area / damage rate of blank control area) x 100

[0068] 4. Data processing

[0069] The test data was analyzed by single factor variance analysis using DPS data processing system, and the difference analysis between different treatment groups was carried out by Duncan's new multiple range method

[0070] 5. Experimental results

[0071] Table 6 Synergistic effect of different concentrations of paclobutrazol and chlorantraniliprole on Chilo suppressalis damage

[0072]

[0073] From Table 6, it can be seen that 50 mg / L, 150 mg / L, 300 mg / L paclobutrazol and kuan-kuan (chlorantraniliprole) can significantly improve the control effect of insecticide on the damage of Chilo suppressalis (Walker) (the average increase of withered stem control effect is 53.2 percentage points, and the average increase of withered heart control effect is 13.8 percentage points), and 50 mg / L, 150 mg / L, 300 mg / L paclobutrazol alone cannot achieve the control effect.

[0074] The above experiments show that paclobutrazol has a synergistic effect on nitenpyram, dinotefuran, sulfoxaflor, pymetrozine and chlorantraniliprole, and mixing with nitenpyram, dinotefuran, sulfoxaflor, pymetrozine and chlorantraniliprole can improve the toxicity, improve the control effect of nitenpyram, dinotefuran, sulfoxaflor, pymetrozine and chlorantraniliprole on resistant insects, and prolong the duration of nitenpyram, dinotefuran, sulfoxaflor, pymetrozine and chlorantraniliprole.

[0075] The above description of disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The application of paclobutrazol as an insecticide synergist, characterized in that: The insecticide is nitenpyram, trifluanid or chlorfenapyr; The synergistic weight ratio of paclobutrazol and nitenpyram is 3:1; The synergistic weight ratio of paclobutrazol and trifluridine is 1:2; The synergistic weight ratio of paclobutrazol and chlorfenapyr is (5:6)-(5:2); The formulation of the insecticide synergist is any one of water-dispersible granules, wettable powders, suspensions, oil suspensions, emulsifiable concentrates, soluble liquids and seed coating agents.

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