A method for cultivating high-yield hybrid shrimp seedlings of Litopenaeus vannamei

Abstract

The present invention discloses a high-yield method for cultivating hybrid shrimp of Penaeus vannamei. The method comprises the following steps: stocking sexually mature broodstock: using common Ecuadorian-sourced Penaeus vannamei as the male parent and "Guangtai No. 1" Penaeus vannamei as the female parent, the broodstock are stocked in net cages within a pond; from the first to the 15th day after the appearance of zoeae, the entire pond is sprayed with a mixture of soybean milk and bacterial protein daily; starting from the 16th day after the appearance of zoeae, feed A daily; when the average body length of the shrimp fry reaches 0.8 to 1.0 cm, feed B daily; and when the average weight of the shrimp fry reaches 2.0 cm or more, feed C daily. The present invention integrates propagation, seedling cultivation, and farming, achieving integrated cultivation of Penaeus vannamei, saving cultivation steps, reducing costs, and achieving high yields. Furthermore, the present invention can improve the shrimp's resistance to WSSV and significantly reduce mortality.

Description

Technical Field

[0001] The invention relates to the technical field of shrimp breeding, and in particular to a method for cultivating high-yield Litopenaeus vannamei hybrid seedlings. Background Art

[0002] Litopenaeus vannamei, belonging to the phylum Arthropoda, class Crustacea, order Decapoda, family Penaeidae, genus Litopenaeus, is one of the most economically viable shrimp species for aquaculture. The most common species of Litopenaeus vannamei available on the market is from Ecuador. Currently, large-scale Litopenaeus vannamei farming typically involves separate seed propagation, seedling rearing, and adult shrimp cultivation. This is primarily due to the varying requirements for cultivation at each stage, making integrated cultivation difficult to achieve in one step and resulting in low yields. Furthermore, common Litopenaeus vannamei from Ecuador suffers from low disease resistance, particularly against common diseases such as White Spot Syndrome Disease (WSSV). Summary of the Invention

[0003] In view of the deficiencies in the prior art, the present invention aims to provide a method for cultivating high-yield hybrid shrimp fry.

[0004] To achieve the purpose of the present invention, the present invention proposes the following technical solutions:

[0005] A method for cultivating high-yield hybrid shrimp seedlings of Litopenaeus vannamei comprises the following steps:

[0006] Stocking of sexually mature broodstock: Using common Ecuadorian-sourced Litopenaeus vannamei as the male parent and Guangtai No. 1 Litopenaeus vannamei as the female parent, the broodstock are stocked in cages within the pond;

[0007] From the 1st to the 15th day after the emergence of zoeae, a mixture of soybean milk and bacterial protein was sprayed throughout the pond every day. From the 16th day after the emergence of zoeae, feed A every day. When the average body length of the shrimp fry reached 0.8-1.0 cm, feed B every day. When the average body weight of the shrimp fry reached more than 2.0 cm, feed C every day.

[0008] Bait A, bait B and bait C all contain bacterial protein.

[0009] Preferably, the method for preparing the bacterial protein comprises the following steps:

[0010] The bacterial liquid of Bacillus amyloliquefaciens is inoculated into a fermentation medium for cultivation. The fermentation liquid is centrifuged to collect bacterial protein, which is then dried for later use.

[0011] Further explanation: The examples of the present invention use the common commercially available Bacillus amyloliquefaciens CICC 20178. When practicing the present invention, Bacillus amyloliquefaciens from other sources can also be selected to achieve the effects of the present invention.

[0012] Preferably, the fermentation medium contains 1-7 g / L of magnesium sulfate and 0.2-5 g / L of potassium chloride.

[0013] Preferably, the fermentation medium contains 5-7 g / L of magnesium sulfate and 0.2-1 g / L of potassium chloride.

[0014] Preferably, the fermentation medium contains 60-70 g / L soybean meal powder, 80-90 g / L corn starch, 5-7 g / L corn steep liquor, 2-3 g / L beef extract, 7-10 g / L peptone, 8-12 g / L glucose, 2-3 g / L trypsin, 6-12 g / L hornwort powder, 1-7 g / L magnesium sulfate, 0.2-5 g / L potassium chloride and water.

[0015] Preferably, in the mixture of soy milk and bacterial protein, the mass ratio of soy milk to bacterial protein is 1:1-2.

[0016] Preferably, based on weight, bait A, bait B and bait C contain 13 to 20 parts of bacterial protein.

[0017] Preferably, bait A contains, by weight: 13 to 20 parts of bacterial protein, 8 to 19 parts of soybean meal, 12 to 27 parts of fish meal, 12 to 20 parts of corn gluten meal, 11 parts of peanut meal, 10 to 15 parts of fish oil, 9 to 10 parts of rice bran powder, 1 to 1.5 parts of complex mineral salts, and 1 to 1.5 parts of complex vitamins.

[0018] Preferably, bait B contains, by weight: 12 to 20 parts of corn gluten meal, 13 to 20 parts of bacterial protein, 12 to 27 parts of fish meal, 8 to 19 parts of soybean meal, 7 to 10 parts of cottonseed meal, 7 to 10 parts of earthworm powder, 5 to 8 parts of high-gluten flour, 5 to 11 parts of peanut meal, 2 to 3 parts of lecithin, 1 to 1.5 parts of complex vitamins, and 1 to 1.5 parts of complex mineral salts.

[0019] Preferably, bait C contains, by weight: 12 to 27 parts of fish meal, 13 to 20 parts of bacterial protein, 12 to 20 parts of corn gluten meal, 9 to 10 parts of rice bran powder, 8 to 19 parts of soybean meal, 7 to 10 parts of cottonseed meal, 5 to 11 parts of peanut meal, 5 to 8 parts of high-gluten flour, 5 to 12 parts of corn oil, 1 to 1.5 parts of complex mineral salts, 1 to 1.5 parts of complex vitamins, and 2 to 3 parts of lecithin.

[0020] Preferably, the method for preparing bacterial protein of the present invention is as follows: Bacillus amyloliquefaciens bacterial liquid is inoculated into a fermentation medium at an inoculum rate of 4% to 6% (volume ratio), and cultured on a shaker at a temperature of 30±2°C for 36 to 48 hours. The fermented broth is centrifuged to collect bacterial protein, and the bacterial protein is dried for later use. The fermentation medium contains 60 to 70 g / L soybean meal powder, 80 to 90 g / L corn starch, 5 to 7 g / L corn steep liquor, 2 to 3 g / L beef extract, 7 to 10 g / L peptone, 8 to 12 g / L glucose, 2 to 3 g / L trypsin, 6 to 12 g / L hornwort powder, 5 to 7 g / L magnesium sulfate, 0.2 to 1 g / L potassium chloride, and water.

[0021] In an embodiment of the present invention, the composite mineral salt contains, by weight, 1200 parts of magnesium sulfate, 240 parts of calcium dihydrogen phosphate, 100 parts of sodium chloride, 60 parts of iron sulfate, 50 parts of zinc sulfate, 50 parts of manganese sulfate, 35 parts of potassium chloride, 10 parts of copper sulfate, and 3 parts of potassium iodide. In an embodiment of the present invention, the composite vitamin contains, by weight, 17 parts of vitamin B1, 20 parts of vitamin B2, 23 parts of vitamin B3, 100 parts of vitamin B5, 10 parts of vitamin B6, and 3 parts of vitamin B 12 0.2 parts, folic acid 5 parts, vitamin B7 0.5 parts, vitamin A 10 parts, vitamin D3 8 parts, vitamin E 900 parts, vitamin C 370 parts, choline chloride 600 parts. In application, the composite mineral salts and composite vitamins can be appropriately adjusted according to actual conditions, including adjustments in composition or content.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The invention integrates propagation, seedling raising and breeding into one, realizes the integrated cultivation of Penaeus vannamei, saves the cultivation process, reduces the cost and increases the shrimp yield.

[0024] The present invention uses common Ecuadorian-sourced Litopenaeus vannamei as the male parent and "Guangtai No. 1" Litopenaeus vannamei as the female parent to obtain offspring hybrid seedlings. During the cultivation process, Bacillus somatic protein obtained by culturing in a high-magnesium, low-potassium fermentation medium containing hornwort is used to improve the shrimp's resistance to WSSV and significantly reduce its mortality. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0026] Example 1

[0027] Cultivation method of hybrid shrimp fry of Penaeus vannamei:

[0028] a. Select a pond of approximately 2 mu (approximately 1.5 acres) as the breeding pond. Dry the pond and expose it to the sun. Remove silt and disinfect it. Fill it with aquaculture seawater to a depth of 1.1 to 1.2 meters. Inoculate the pond with Chlorella vulgaris to maintain an algae density of no less than 50,000 cells / mL. Apply 200 kg of decomposed manure per mu (approximately 1.5 to 2.5 acres) before stocking the prawns.

[0029] b. Stocking of broodstock: Stock sexually mature shrimps, using common Ecuadorian-sourced Litopenaeus vannamei as the male parent and "Guangtai No. 1" Litopenaeus vannamei as the female parent, in a male-to-female ratio of 5:1 in pond cages (with aquatic plants covering one-third of the cage volume). Stock 15 kg of mixed stock per mu (approximately 15 kg) and feed them with common adult shrimp feed. Once juvenile shrimp appear in the water, recapture the broodstock.

[0030] c. Water quality requirements during the cultivation period: water salinity is 28-32‰, water pH value is 7.8-8.2, dissolved oxygen in water is above 5mg / L, and transparency is 40-60cm.

[0031] d. Feeding and management during the cultivation period:

[0032] From the 1st to the 15th day after the appearance of zoeae, a mixture of soybean milk and bacterial protein (mass ratio 1:1) is sprayed throughout the pond every day, and the daily feeding amount is 2kg / mu; starting from the 16th day after the appearance of zoeae, feed A is fed every day, and the feeding amount is 3kg / mu; when the average body length of the shrimp fry reaches 0.8-1.0cm, feed B every day, and the feeding amount is 5kg / mu; when the average weight of the shrimp fry reaches more than 2.0cm, feed C every day, and the feeding amount is 6% of the shrimp body weight.

[0033] Bait A contains (by weight): 20 parts of bacterial protein, 19 parts of soybean meal, 12 parts of fish meal, 12 parts of corn gluten meal, 11 parts of peanut meal, 10 parts of fish oil, 10 parts of rice bran powder, 1 part of complex mineral salts, and 1 part of complex vitamins.

[0034] Bait B contains (by weight): 20 parts of corn gluten meal, 15 parts of bacterial protein, 12 parts of fish meal, 10 parts of soybean meal, 10 parts of cottonseed meal, 10 parts of earthworm powder, 8 parts of high-gluten flour, 6 parts of peanut meal, 3 parts of lecithin, 1.5 parts of complex vitamins, and 1 part of complex mineral salts.

[0035] Bait C contains (by weight): 17 parts of fish meal, 13 parts of bacterial protein, 13 parts of corn gluten meal, 10 parts of bacterial protein, 9 parts of rice bran powder, 8 parts of soybean meal, 7 parts of cottonseed meal, 5 parts of peanut meal, 5 parts of high-gluten flour, 5 parts of corn oil, 1 part of complex mineral salts, 1 part of complex vitamins, and 2 parts of lecithin.

[0036] The method for preparing bacterial protein in this example is as follows: Bacillus amyloliquefaciens bacterial solution (10 8 cfu / mL) was inoculated into a fermentation medium at an inoculum size of 6% (volume ratio) and cultured on a shaker at 150 rpm for 36 to 48 hours at 30±2°C. The fermentation broth was centrifuged (4000 rpm) to collect bacterial protein, which was then dried at 40°C for later use. The fermentation medium contained 70 g / L soybean meal, 85 g / L corn starch, 5 g / L corn steep liquor, 2 g / L beef extract, 10 g / L peptone, 8 g / L glucose, 3 g / L trypsin, 10 g / L hornwort powder, 7 g / L magnesium sulfate, 1 g / L potassium chloride, and water.

[0037] After three months of cultivation, the shrimp yield reached more than 140kg / mu.

[0038] Example 2

[0039] In this embodiment, the bacterial protein was replaced by corn gluten meal.

[0040] Cultivation method of hybrid shrimp fry of Penaeus vannamei:

[0041] a. Select a pond of approximately 2 mu (approximately 1.5 acres) as the breeding pond. Dry the pond and expose it to the sun. Remove silt and disinfect it. Fill it with aquaculture seawater to a depth of 1.1 to 1.2 meters. Inoculate the pond with Chlorella vulgaris to maintain an algae density of no less than 50,000 cells / mL. Apply 200 kg of decomposed manure per mu (approximately 1.5 to 2.5 acres) before stocking the prawns.

[0042] b. Stocking of broodstock: Stock sexually mature shrimps, using common Ecuadorian-sourced Litopenaeus vannamei as the male parent and "Guangtai No. 1" Litopenaeus vannamei as the female parent, in a male-to-female ratio of 5:1 in pond cages (with aquatic plants covering one-third of the cage volume). Stock 15 kg of mixed stock per mu (approximately 15 kg) and feed them with common adult shrimp feed. Once juvenile shrimp appear in the water, recapture the broodstock.

[0043] c. Water quality requirements during the cultivation period: water salinity is 28-32‰, water pH value is 7.8-8.2, dissolved oxygen in water is above 5mg / L, and transparency is 40-60cm.

[0044] d. Feeding and management during the cultivation period:

[0045] From the 1st to the 15th day after the emergence of zoeae, a mixture of soybean milk and corn gluten meal (mass ratio 1:1) is sprayed throughout the pond every day, and the daily feeding amount is 2kg / mu; starting from the 16th day after the emergence of zoeae, feed A every day, and the feeding amount is 3kg / mu; when the average body length of the shrimp fry reaches 0.8-1.0cm, feed B every day, and the feeding amount is 5kg / mu; when the average weight of the shrimp fry reaches more than 2.0cm, feed C every day, and the feeding amount is 6% of the shrimp body weight.

[0046] Bait A contains (by weight): 19 parts of soybean meal, 12 parts of fish meal, 32 parts of corn gluten meal, 11 parts of peanut meal, 10 parts of fish oil, 10 parts of rice bran powder, 1 part of complex mineral salts, and 1 part of complex vitamins.

[0047] Bait B contains (by weight): 35 parts of corn gluten meal, 12 parts of fish meal, 10 parts of soybean meal, 10 parts of cottonseed meal, 10 parts of earthworm powder, 8 parts of high-gluten flour, 6 parts of peanut meal, 3 parts of lecithin, 1.5 parts of complex vitamins, and 1 part of complex mineral salts.

[0048] Bait C contains (by weight): 27 parts of fish meal, 26 parts of corn gluten meal, 9 parts of rice bran powder, 8 parts of soybean meal, 7 parts of cottonseed meal, 5 parts of peanut meal, 5 parts of high-gluten flour, 5 parts of corn oil, 1 part of complex mineral salts, 1 part of complex vitamins, and 2 parts of lecithin.

[0049] After three months of cultivation, the shrimp yield reached more than 120kg / mu.

[0050] Example 3

[0051] In this embodiment, bacterial protein prepared in a low-magnesium, high-potassium culture medium was used.

[0052] Cultivation method of hybrid shrimp fry of Penaeus vannamei:

[0053] a. Select a pond of approximately 2 mu (approximately 1.5 acres) as the breeding pond. Dry the pond and expose it to the sun. Remove silt and disinfect it. Fill it with aquaculture seawater to a depth of 1.1 to 1.2 meters. Inoculate the pond with Chlorella vulgaris to maintain an algae density of no less than 50,000 cells / mL. Apply 200 kg of decomposed manure per mu (approximately 1.5 to 2.5 acres) before stocking the prawns.

[0054] b. Stocking of broodstock: Stock sexually mature shrimps, using common Ecuadorian-sourced Litopenaeus vannamei as the male parent and "Guangtai No. 1" Litopenaeus vannamei as the female parent, in a male-to-female ratio of 5:1 in pond cages (with aquatic plants covering one-third of the cage volume). Stock 15 kg of mixed stock per mu (approximately 15 kg) and feed them with common adult shrimp feed. Once juvenile shrimp appear in the water, recapture the broodstock.

[0055] c. Water quality requirements during the cultivation period: water salinity is 28-32‰, water pH value is 7.8-8.2, dissolved oxygen in water is above 5mg / L, and transparency is 40-60cm.

[0056] d. Feeding and management during the cultivation period:

[0057] From the 1st to the 15th day after the appearance of zoeae, a mixture of soybean milk and bacterial protein (mass ratio 1:1) is sprayed throughout the pond every day, and the daily feeding amount is 2kg / mu; starting from the 16th day after the appearance of zoeae, feed A is fed every day, and the feeding amount is 3kg / mu; when the average body length of the shrimp fry reaches 0.8-1.0cm, feed B every day, and the feeding amount is 5kg / mu; when the average weight of the shrimp fry reaches more than 2.0cm, feed C every day, and the feeding amount is 6% of the shrimp body weight.

[0058] Bait A contains (by weight): 20 parts of bacterial protein, 19 parts of soybean meal, 12 parts of fish meal, 12 parts of corn gluten meal, 11 parts of peanut meal, 10 parts of fish oil, 10 parts of rice bran powder, 1 part of complex mineral salts, and 1 part of complex vitamins.

[0059] Bait B contains (by weight): 20 parts of corn gluten meal, 15 parts of bacterial protein, 12 parts of fish meal, 10 parts of soybean meal, 10 parts of cottonseed meal, 10 parts of earthworm powder, 8 parts of high-gluten flour, 6 parts of peanut meal, 3 parts of lecithin, 1.5 parts of complex vitamins, and 1 part of complex mineral salts.

[0060] Bait C contains (by weight): 27 parts of fish meal, 13 parts of bacterial protein, 13 parts of corn gluten meal, 9 parts of rice bran powder, 8 parts of soybean meal, 7 parts of cottonseed meal, 5 parts of peanut meal, 5 parts of high-gluten flour, 5 parts of corn oil, 1 part of complex mineral salts, 1 part of complex vitamins, and 2 parts of lecithin.

[0061] The method for preparing bacterial protein in this example is as follows: Bacillus amyloliquefaciens bacterial solution (10 8 cfu / mL) was inoculated into a fermentation medium at an inoculum size of 6% (volume ratio), and cultured on a shaker at 150 rpm for 36 to 48 hours at a temperature of 30±2°C. The fermentation broth was centrifuged (4000 rpm), bacterial protein was collected, and the bacterial protein was dried at 40°C for later use. The fermentation medium contained 70 g / L soybean meal powder, 85 g / L corn starch, 5 g / L corn steep liquor, 2 g / L beef extract, 10 g / L peptone, 8 g / L glucose, 3 g / L trypsin, 10 g / L hornwort powder, 1 g / L magnesium sulfate, 5 g / L potassium chloride, and water.

[0062] After three months of cultivation, the shrimp yield reached more than 140kg / mu.

[0063] Example 4

[0064] In this example, the common Ecuadorian shrimp Litopenaeus vannamei was cultivated.

[0065] Cultivation method of Penaeus vannamei fry:

[0066] a. Select a pond of approximately 2 mu (approximately 1.5 acres) as the breeding pond. Dry the pond and expose it to the sun. Remove silt and disinfect it. Fill it with aquaculture seawater to a depth of 1.1 to 1.2 meters. Inoculate the pond with Chlorella vulgaris to maintain an algae density of no less than 50,000 cells / mL. Apply 200 kg of decomposed manure per mu (approximately 1.5 to 2.5 acres) before stocking the prawns.

[0067] b. Stocking of broodstock: Stock sexually mature shrimp, using common Ecuadorian Litopenaeus vannamei as the parent stock, in pond cages (with aquatic plants covering one-third of the cage volume) at a female-to-male ratio of 3-5:1. Stock 15 kg of mixed stock per mu (approximately 15 kg) and feed common adult shrimp feed. Once juvenile shrimp appear in the water, recapture the broodstock.

[0068] c. Water quality requirements during the cultivation period: water salinity is 28-32‰, water pH value is 7.8-8.2, dissolved oxygen in water is above 5mg / L, and transparency is 40-60cm.

[0069] d. Feeding and management during the cultivation period:

[0070] From the 1st to the 15th day after the emergence of zoeae, a mixture of soybean milk and bacterial protein (mass ratio 1:1) is sprayed throughout the pond every day, and the daily feeding amount is 1-2 kg / mu; starting from the 16th day after the emergence of zoeae, feed A every day, and the feeding amount is 3 kg / mu; when the average body length of the shrimp fry reaches 0.8-1.0 cm, feed B every day, and the feeding amount is 5 kg / mu; when the average weight of the shrimp fry reaches more than 2.0 cm, feed C every day, and the feeding amount is 6% of the shrimp body weight.

[0071] Bait A contains (by weight): 20 parts of bacterial protein, 19 parts of soybean meal, 12 parts of fish meal, 12 parts of corn gluten meal, 11 parts of peanut meal, 10 parts of fish oil, 10 parts of rice bran powder, 1 part of complex mineral salts, and 1 part of complex vitamins.

[0072] Bait B contains (by weight): 20 parts of corn gluten meal, 15 parts of bacterial protein, 12 parts of fish meal, 10 parts of soybean meal, 10 parts of cottonseed meal, 10 parts of earthworm powder, 8 parts of high-gluten flour, 6 parts of peanut meal, 3 parts of lecithin, 1.5 parts of complex vitamins, and 1 part of complex mineral salts.

[0073] Bait C contains (by weight): 17 parts of fish meal, 13 parts of bacterial protein, 13 parts of corn gluten meal, 10 parts of bacterial protein, 9 parts of rice bran powder, 8 parts of soybean meal, 7 parts of cottonseed meal, 5 parts of peanut meal, 5 parts of high-gluten flour, 5 parts of corn oil, 1 part of complex mineral salts, 1 part of complex vitamins, and 2 parts of lecithin.

[0074] After three months of cultivation, the shrimp yield reached more than 120kg / mu.

[0075] Example 5

[0076] In this embodiment, the shrimp Litopenaeus vannamei of Ecuador was cultivated, and the bacterial protein was replaced by corn gluten powder. generation.

[0077] Cultivation method of Penaeus vannamei fry:

[0078] a. Select a pond of approximately 2 mu (approximately 1.5 acres) as the breeding pond. Dry the pond and expose it to the sun. Remove silt and disinfect it. Fill it with aquaculture seawater to a depth of 1.1 to 1.2 meters. Inoculate the pond with Chlorella vulgaris to maintain an algae density of no less than 50,000 cells / mL. Apply 200 kg of decomposed manure per mu (approximately 1.5 to 2.5 acres) before stocking the prawns.

[0079] b. Stocking of broodstock: Stock sexually mature shrimp, using common Ecuadorian Litopenaeus vannamei as the parent stock, in pond cages (with aquatic plants covering one-third of the cage volume) at a female-to-male ratio of 3-5:1. Stock 15 kg of mixed stock per mu (approximately 15 kg) and feed common adult shrimp feed. Once juvenile shrimp appear in the water, recapture the broodstock.

[0080] c. Water quality requirements during the cultivation period: water salinity is 28-32‰, water pH value is 7.8-8.2, dissolved oxygen in water is above 5mg / L, and transparency is 40-60cm.

[0081] d. Feeding and management during the cultivation period:

[0082] From the 1st to the 15th day after the emergence of zoeae, a mixture of soybean milk and corn gluten meal (mass ratio 1:1) is sprayed throughout the pond every day, and the daily feeding amount is 1-2 kg / mu; starting from the 16th day after the emergence of zoeae, feed bait A every day, and the feeding amount is 3 kg / mu; when the average body length of the shrimp fry reaches 0.8-1.0 cm, feed bait B every day, and the feeding amount is 5 kg / mu; when the average weight of the shrimp fry reaches more than 2.0 cm, feed bait C every day, and the feeding amount is 6% of the shrimp body weight.

[0083] Bait A contains (by weight): 19 parts of soybean meal, 12 parts of fish meal, 32 parts of corn gluten meal, 11 parts of peanut meal, 10 parts of fish oil, 10 parts of rice bran powder, 1 part of complex mineral salts, and 1 part of complex vitamins.

[0084] Bait B contains (by weight): 35 parts of corn gluten meal, 12 parts of fish meal, 10 parts of soybean meal, 10 parts of cottonseed meal, 10 parts of earthworm powder, 8 parts of high-gluten flour, 6 parts of peanut meal, 3 parts of lecithin, 1.5 parts of complex vitamins, and 1 part of complex mineral salts.

[0085] Bait C contains (by weight): 17 parts of fish meal, 26 parts of corn gluten meal, 10 parts of bacterial protein, 9 parts of rice bran powder, 8 parts of soybean meal, 7 parts of cottonseed meal, 5 parts of peanut meal, 5 parts of high-gluten flour, 5 parts of corn oil, 1 part of complex mineral salts, 1 part of complex vitamins, and 2 parts of lecithin.

[0086] After three months of cultivation, the shrimp yield reached more than 110kg / mu.

[0087] Poison attack experiment:

[0088] WSSV poisonous feed: Take the muscles of WSSV-infected Litopenaeus vannamei and cut them into particles of similar size to feed particles under low temperature and sterile conditions.

[0089] Before the experiment, shrimp cultured for 60 days in each example or comparative example were temporarily reared in an aquarium for 7 days. During the experiment, the water was changed once daily, the shrimp were fed three times, and the aeration was continuous. The water temperature was 25-28°C, the salinity was 31-32‰, and the pH was 7.8-8.0. After the temporary rearing, uniform, healthy, and active shrimp were selected and randomly divided into groups. Each group had three replicates, with 100 shrimp in each replicate. The day before the experiment, the normal feed intake was reduced by half to facilitate shrimp ingestion of the toxic feed. On the first day of the experiment, the shrimp were fed 0.1 g of toxic feed pellets per shrimp. Normal rearing was then continued. The mortality rate was counted daily, and dead shrimp were removed from the aquarium. The following table shows the mortality rate of each group on the seventh day of the experiment.

[0090] Table 1

[0091] mortality rate(%) Example 1 7.7±0.58％ Example 2 36.0±2.65％ Example 3 23.7±2.08％ Example 4 30.0±2.65％ Example 5 54.0±5.29％

[0092] The experimental results showed that the mortality rate of hybrid shrimp was significantly reduced compared to shrimp fry with common Ecuadorian Litopenaeus vannamei as the parent (p < 0.05). Comparisons with Examples 1, 2, and 3 show that bacterial protein produced in a high-magnesium, low-potassium culture medium can help reduce the mortality rate of hybrid shrimp. Based on these results, the following conclusions were drawn: hybrid shrimp progeny obtained from common Ecuadorian Litopenaeus vannamei as the male parent and "Guangtai No. 1" Litopenaeus vannamei as the female parent, and bacterial protein produced in a high-magnesium, low-potassium culture medium during cultivation, can improve the shrimp's resistance to WSSV and significantly reduce mortality.

[0093] The above description is merely an example of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made based on the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for cultivating high-yield hybrid shrimp seedlings of Penaeus vannamei, characterized in that: The following steps are involved: Stocking of sexually mature broodstock: Using common Ecuadorian Litopenaeus vannamei as the male parent and Guangtai No. 1 Litopenaeus vannamei as the female parent, the broodstock are stocked in cages within the pond; From the 1st to the 15th day after the emergence of zoeae, a mixture of soybean milk and bacterial protein was sprayed throughout the pond daily. Starting from the 16th day after the emergence of zoeae, feed A daily. When the average body length of the shrimp fry reached 0.8-1.0 cm, feed B daily. When the average body weight of the shrimp fry reached more than 2.0 cm, feed C daily. Bait A, Bait B and Bait C all contain bacterial protein; The preparation method of the bacterial protein comprises the following steps: Inoculating a Bacillus amyloliquefaciens bacterial liquid into a fermentation medium for cultivation, centrifuging the fermentation liquid after fermentation, collecting bacterial protein, and drying the bacterial protein for later use; The fermentation medium contains 5-7 g / L of magnesium sulfate and 0.2-1 g / L of potassium chloride; In the soy milk and bacterial protein mixture, the mass ratio of soy milk to bacterial protein is 1:1-2; The bait A, bait B and bait C contain 13 to 20 parts of bacterial protein by weight; By weight, bait A contains: 13-20 parts of bacterial protein, 8-19 parts of soybean meal, 12-27 parts of fish meal, 12-20 parts of corn gluten meal, 11 parts of peanut meal, 10-15 parts of fish oil, 9-10 parts of rice bran powder, 1-1.5 parts of complex mineral salts, and 1-1.5 parts of complex vitamins; In parts by weight, bait B contains: 12-20 parts of corn gluten meal, 13-20 parts of bacterial protein, 12-27 parts of fish meal, 8-19 parts of soybean meal, 7-10 parts of cottonseed meal, 7-10 parts of earthworm meal, 5-8 parts of high-gluten flour, 5-11 parts of peanut meal, 2-3 parts of lecithin, 1-1.5 parts of complex vitamins, and 1-1.5 parts of complex mineral salts; By weight, bait C contains: 12-27 parts of fish meal, 13-20 parts of bacterial protein, 12-20 parts of corn gluten meal, 9-10 parts of rice bran powder, 8-19 parts of soybean meal, 7-10 parts of cottonseed meal, 5-11 parts of peanut meal, 5-8 parts of high-gluten flour, 5-12 parts of corn oil, 1-1.5 parts of complex mineral salts, 1-1.5 parts of complex vitamins, and 2-3 parts of lecithin.

2. The method for cultivating high-yield Litopenaeus vannamei hybrid seedlings according to claim 1, wherein: The fermentation medium contains 60-70 g / L soybean meal powder, 80-90 g / L corn starch, 5-7 g / L corn steep liquor, 2-3 g / L beef extract, 7-10 g / L peptone, 8-12 g / L glucose, 2-3 g / L trypsin, 6-12 g / L hornwort powder, 5-7 g / L magnesium sulfate, 0.2-1 g / L potassium chloride and water.