Pearl peptide with whitening and antioxidant efficacy, preparation method and application thereof

Abstract

The present application relates to the technical field of polypeptide, and discloses a pearl producing Pinctada martensii with whitening and antioxidant effects, a preparation method and application thereof, wherein the pearl producing Pinctada martensii is a mixture of short peptides, the pearl producing Pinctada martensii takes pearl layer powder of Pinctada martensii as raw material, water and composite ionic liquid are added in proportion, and after physical modification under high temperature and high pressure, the pearl producing Pinctada martensii is sequentially subjected to complex degradation by bromelain and central protease, enzyme inactivation, centrifugation, separation and purification, and identification. The pearl producing Pinctada martensii prepared by the present application has the effects of whitening and free radical scavenging, and the present application provides a new type of bioactive peptide for the field of cosmetics, and simultaneously improves the application value of pearl layer powder in the fields of biological medicine and food.

Description

Technical Field

[0001] The present invention belongs to the technical field of polypeptide preparation, and specifically relates to the preparation and application of pearl peptide with whitening and anti-oxidation effects. Background Art

[0002] Nacre is a layer of lustrous, off-white pearl secreted by mantle cells and found inside pearl shells. Nacre powder is produced by grinding it into a fine powder. Nacre and pearls are homogenous and share the same origin. Research has shown that the main components of nacre powder are very similar to those of pearl powder: inorganic components, organic components, and water. The inorganic component content is 90%-96%, the organic content is approximately 2.0%-7.0%, and the water content is approximately 0.2%-2.0%. Nacre powder is often used in cosmetics as a substitute for more expensive pearl powder, offering similar benefits. Pearls have long been a favorite for whitening and nourishing the skin. The Compendium of Materia Medica records that pearls have numerous benefits, including "salty, sweet, cold, and non-toxic properties. They calm the mind, enhance complexion, remove facial blemishes, stop diarrhea, detoxify acne, and enhance radiance and whitening." Modern medical research further confirms the benefits of pearls and nacre powder, including improving eyesight, calming the nerves, detoxifying, and whitening. Currently, pearls are included in the State Food and Drug Administration's catalog of cosmetic ingredients as a key ingredient in whitening and anti-freckle products.

[0003] Pearl protein and its enzymatically hydrolyzed active peptides are the primary components of pearl's whitening properties. The main component of nacreous protein is shell keratin, which binds firmly to organic and inorganic matter, forming a strong cross-linked network. Furthermore, shell keratin is a complex structure, with interactions such as hydrogen bonds, hydrophobic bonds, and disulfide bonds resulting in tightly coiled peptide chains. This makes it difficult to dissolve in water and thus impractical for direct utilization. Furthermore, it presents challenges such as difficulty in degradation and low extraction yield of small-molecule peptides. Enzymatic hydrolysis to produce pearl active peptides is one approach to achieving high-value utilization of pearl protein. Therefore, the efficient degradation of nacreous protein and the development of pearl active peptides with potent whitening properties hold broad application prospects in the field of skin-related biomedicine and will generate significant economic value.

[0004] Currently, the main methods for developing and applying functional components from nacre include traditional water extraction, acid hydrolysis, and direct enzymatic hydrolysis. Traditional water extraction cannot effectively utilize the insoluble pearl protein. While acid hydrolysis can effectively degrade shell keratin, it mostly degrades it into amino acids, destroying the functional components. Direct enzymatic hydrolysis suffers from drawbacks such as low efficiency, low yield of small-molecule peptides, and high cost. Furthermore, there are currently no reports on the extraction of tyrosinase inhibitory peptides and anti-photoaging peptides from nacre powder of the Pinctada martensii oyster.

[0005] In addition, in the prior art, small molecule peptides are generally separated by liquid chromatography, which not only has complex separation and purification conditions but also has low separation efficiency and high cost, and cannot be applied to industrial production. SUMMARY

[0006] Therefore, in order to further efficiently utilize the nacreous layer powder protein resource of seawater and tap new functional activity, the application provides a preparation method of pearl peptide of Pinctada martensii with whitening activity and antioxidant effect.

[0007] The present application provides the following technical solutions:

[0008] A pearl peptide with whitening and antioxidant effects, characterized in comprising the following polypeptides:

[0009] NPPMP1: Thr-Phe-Ser-Gly-Asn-Tyr-Pro;

[0010] NPPMP2: Gly-Gly-Phe-Gly-Asn-Trp;

[0011] NPPMP3: Ala-Thr-Phe-Asp-Ala-Ile;

[0012] NPPMP4: Leu-Lys-Gly-His-Glu-Asp-Leu;

[0013] NPPMP5: His-Ser-Ser-Ala-His-Ser;

[0014] NPPMP6: Gly-Gly-Ser-Phe-Ser-Val-Arg;

[0015] The pearl peptide is a pearl peptide of Pinctada martensii.

[0016] The application further provides a preparation method of the pearl peptide, characterized by comprising the following steps:

[0017] S1. After Pinctada martensii nacreous layer powder is added into distilled water in a proper proportion, a composite ionic liquid is added, and then high-temperature and high-pressure treatment is performed, and then a composite protease is added in a proper proportion for enzymolysis, and after enzyme inactivation, centrifugation is performed to obtain supernatant;

[0018] S2. After the supernatant of step S1 is decolorized, small molecular components are intercepted by using an ultrafiltration membrane, and then vacuum concentration and freeze-drying are performed to obtain small molecular Pinctada martensii pearl peptide.

[0019] Further, in step S1, the composite ionic liquid comprises the following components in parts by weight: soybean polysaccharide 39-57, sodium dodecyl sulfate 15-22, aspartic acid 18-24, and glutamic acid 11-19.

[0020] Further, in step S1, the addition amount of the composite ionic liquid is 23-35% of the mass of the nacreous layer powder.

[0021] In the composite ionic liquid used in the present invention:

[0022] Soybean polysaccharides are water-soluble polysaccharides produced from soybeans or soybean meal through processes such as defatting, extraction, decolorization, purification, and drying. They inhibit lipid oxidation and stabilize protein in acidic beverages, improving food quality, processing properties, and appearance. Water-soluble soy polysaccharides have low viscosity and can be formulated into high-concentration solutions, the viscosity of which is virtually unaffected by salts. Soybean polysaccharides exhibit diverse properties, including dispersibility, stabilization, emulsification, and adhesion.

[0023] Sodium lauryl sulfate is a non-toxic anionic surfactant with a biodegradability of >90%. It has good compatibility with anions and non-ions, and has excellent emulsification, foaming, penetration and dispersion properties. It can destroy non-covalent bonds such as ionic bonds and hydrogen bonds in proteins.

[0024] Aspartic acid and glutamic acid are acidic amino acids.

[0025] The composite ionic liquid of the present invention can, under physical modification conditions, synergistically compound the various components, reacting with calcium carbonate through the combination of aspartic acid and glutamic acid to form soluble calcium ions, and preventing the formation of precipitation under the action of sodium lauryl sulfate. Under the action of soybean polysaccharides, a stable reaction system is formed to prevent the occurrence of reversible reactions. Under high temperature and high pressure conditions, the composite ionic liquid reacts with the internal structure of the nacreous layer powder of the Pinctada martensii oyster, penetrates into the network structure between the organic and inorganic matter and interacts with it, loosening the bond between the organic and inorganic matter, and has a certain destructive effect on the hydrogen bonds, hydrophobic bonds, disulfide bonds, etc. within the shell keratin, so that the shell protein of the nacreous layer powder can be more fully degraded into small molecule peptides with biological activity during subsequent enzymatic hydrolysis, thereby realizing the high-value utilization of the nacreous layer powder.

[0026] Furthermore, in step S1, distilled water is added to the nacreous layer powder of Pinctada martensii in a ratio of 1:3-1:5, the treatment pressure is controlled at 0.20-0.25 MPa, the reaction temperature is controlled at 115-121° C., and the physical modification treatment is carried out for 20-30 min.

[0027] In the present invention, high temperature conditions facilitate the decomposition of reactants and the formation of free radicals, thereby increasing the chemical reaction rate. Under high pressure, the high pressure generated by the collapse of bubbles is accompanied by a strong shock wave, which has a significant impact on heterogeneous systems containing solids, leading to strong collisions and aggregation between molecules. The combination of high temperature and high pressure provides unique reaction conditions for chemical reactions that are difficult or impossible to achieve under normal conditions.

[0028] Furthermore, in step S1, the composite enzyme selected for enzymatic hydrolysis is a combination of bromelain and neutral protease bromelain, and simultaneous enzymatic hydrolysis is adopted. The amount of bromelain added is 0.10-0.30 g / 100g, and the amount of neutral protease bromelain added is 0.2-0.6 g / 100g. The pH is adjusted to 6.0-7.0 and the temperature is 45-60°C. After enzymatic hydrolysis for 4-7 hours, the enzyme is inactivated at 100°C for 20 minutes, and the supernatant is obtained by centrifugation.

[0029] Through the composite enzymatic hydrolysis reaction, the efficiency of enzymatic hydrolysis and the yield of small molecule active substances can be improved, and the extracted small molecule active peptides are highly active and targeted, giving them specific biological activity.

[0030] Furthermore, in step S2, activated carbon and diatomaceous earth are used for combined decolorization. After filtration, the filtrate is further filtered using an ultrafiltration membrane to retain components with a molecular weight less than 3000 Da.

[0031] The innovation of the present invention lies in that, by adding composite ionic liquid, under high temperature and high pressure conditions, on the one hand, the release rate of small molecule active substances can be increased; on the other hand, the proportion of small molecule active peptide components can be controlled by the amount of addition, making them more prominent in certain biologically active functions, which can meet the needs of different application scenarios.

[0032] The present invention also provides an application of the pearl peptide in preparing a product for scavenging free radicals and inhibiting tyrosinase.

[0033] In the present invention, by combining the intervention of composite ionic liquid with high temperature and high pressure pretreatment, the content of soluble small molecule peptides prepared by enzymatic hydrolysis can be effectively increased. Compared with the conventional enzymatic hydrolysis preparation in the prior art, the degree of hydrolysis is significantly improved; moreover, composite enzymatic hydrolysis can significantly improve the efficiency of hydrolyzing insoluble proteins.

[0034] Through a large number of creative experiments, the applicant discovered that a reasonable pre-treatment method and a combination of proteases play a key role in degrading insoluble pearl protein, and can solve technical problems in the existing technology such as poor water solubility and difficulty in utilization of seawater nacre protein.

[0035] Furthermore, through the intervention of the composite ionic liquid of the present invention, under the same preparation conditions, a stable composition of pearl peptides with specific sequences can be obtained, with the proportion of each sequence controlled within the desired range. The target product can be obtained by ultrafiltration membrane interception, ensuring that the product has the desired biological activity. This effectively solves the problems of the existing technology of small molecule peptide separation and purification, such as complex conditions, high costs, low separation efficiency, and the inability to achieve industrial production.

[0036] The pearl peptide prepared by the present invention has high tyrosinase inhibitory activity, has the effect of scavenging free radicals, is highly safe, and has no toxic side effects. It can solve the technical problem that traditional tyrosinase inhibitors such as kojic acid and arbutin in the prior art may have toxic side effects.

[0037] The preparation method of the present invention can effectively improve the added value of Pinctada martensii shell waste and enhance the application value of seawater nacre powder in the fields of cosmetics, biomedicine and food. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 LC-MS total ion pattern for separation and identification of pearl peptides from Pinctada martensii. DETAILED DESCRIPTION

[0039] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0040] Example 1

[0041] A pearl peptide with whitening and antioxidant effects, characterized by comprising the following polypeptides:

[0042] NPPMP1: Thr-Phe-Ser-Gly-Asn-Tyr-Pro;

[0043] NPPMP2: Gly-Gly-Phe-Gly-Asn-Trp;

[0044] NPPMP3: Ala-Thr-Phe-Asp-Ala-Ile;

[0045] NPPMP4: Leu-Lys-Gly-His-Glu-Asp-Leu;

[0046] NPPMP5: His-Ser-Ser-Ala-His-Ser;

[0047] NPPMP6: Gly-Gly-Ser-Phe-Ser-Val-Arg;

[0048] The pearl peptide is Pinctada martensii pearl peptide.

[0049] Example 2

[0050] A method for preparing pearl peptide according to embodiment 1 is characterized by comprising the following steps:

[0051] S1. Washed and dried Pinctada martensii nacre powder (200-400 mesh) was added to distilled water in appropriate proportions, followed by a composite ionic liquid. After high-temperature and high-pressure treatment, a composite protease was added in appropriate proportions for enzymatic hydrolysis. The enzyme was inactivated and the supernatant was obtained by centrifugation.

[0052] S2. After decolorization, the supernatant of step S1 is subjected to ultrafiltration membrane to intercept small molecule components, and then vacuum concentrated and freeze-dried to obtain small molecule Pinctada martensii pearl peptide.

[0053] Furthermore, in step S1, the composite ionic liquid includes the following components in parts by weight: 45 parts soybean polysaccharide, 18 parts sodium lauryl sulfate, 21 parts aspartic acid, and 15 parts glutamic acid.

[0054] Furthermore, in step S1, the amount of the composite ionic liquid added is 27% of the mass of the nacreous layer powder.

[0055] Furthermore, in step S1, the nacreous layer powder of Pinctada martensii was added to distilled water in a ratio of 1:4, the treatment pressure was controlled at 0.22 MPa, the reaction temperature was controlled at 120° C., and the physical modification treatment was performed for 25 minutes.

[0056] Furthermore, in step S1, the composite enzyme selected for enzymatic hydrolysis is a combination of bromelain and neutral protease bromelain, and simultaneous enzymatic hydrolysis is adopted. The amount of bromelain added is 0.20 g / 100 g, and the amount of neutral protease bromelain added is 0.4 g / 100 g. The pH is adjusted to 6.5 and the temperature is 50° C. After enzymatic hydrolysis for 5 h, the enzyme is inactivated at 100° C. for 20 min, and the supernatant is obtained by centrifugation.

[0057] Furthermore, in step S2, activated carbon and diatomaceous earth are used for combined decolorization. After filtration, the filtrate is further filtered using an ultrafiltration membrane to retain components with a molecular weight less than 3000 Da.

[0058] Example 3

[0059] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that in step S1, the amount of the composite ionic liquid added is 23% of the mass of the nacre powder.

[0060] Example 4

[0061] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that in step S1, the amount of the composite ionic liquid added is 30% of the mass of the pearl layer powder.

[0062] Example 5

[0063] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that in step S1, the amount of the composite ionic liquid added is 35% of the mass of the pearl layer powder.

[0064] Example 6

[0065] This embodiment provides a preparation method of pearl peptides that is the same as that of embodiment 2, except that in step S1, the composite ionic liquid includes the following components in parts by weight: 41 parts soybean polysaccharide, 17 parts sodium lauryl sulfate, 19 parts aspartic acid, and 13 parts glutamic acid.

[0066] Example 7

[0067] This embodiment provides a preparation method of pearl peptides that is the same as that of embodiment 2, except that in step S1, the composite ionic liquid includes the following components in parts by weight: 55 parts soybean polysaccharide, 20 parts sodium lauryl sulfate, 23 parts aspartic acid, and 17 parts glutamic acid.

[0068] Example 8

[0069] This embodiment provides a preparation method of pearl peptides that is the same as that of embodiment 2, except that in step S1, the composite ionic liquid includes the following components in parts by weight: 39 parts soybean polysaccharide, 15 parts sodium lauryl sulfate, 18 parts aspartic acid, and 11 parts glutamic acid.

[0070] Furthermore, in step S1, the nacreous layer powder of Pinctada martensii was added to distilled water in a ratio of 1:3, the treatment pressure was controlled at 0.20 MPa, the reaction temperature was controlled at 121° C., and the physical modification treatment was performed for 20 min.

[0071] Furthermore, in step S1, the composite enzyme selected for enzymatic hydrolysis is a combination of bromelain and neutral protease bromelain, and simultaneous enzymatic hydrolysis is adopted. The amount of bromelain added is 0.10 g / 100 g, and the amount of neutral protease bromelain added is 0.2 g / 100 g. The pH is adjusted to 7.0 and the temperature is 45° C. After enzymatic hydrolysis for 6 h, the enzyme is inactivated at 100° C. for 20 min, and the supernatant is obtained by centrifugation.

[0072] Example 9

[0073] This embodiment provides a preparation method of pearl peptides that is the same as that of embodiment 2, except that in step S1, the composite ionic liquid includes the following components in parts by weight: 57% soybean polysaccharide, 22% sodium lauryl sulfate, 24% aspartic acid, and 19% glutamic acid.

[0074] Furthermore, in step S1, the nacreous layer powder of Pinctada martensii was added to distilled water in a ratio of 1:5, the treatment pressure was controlled at 0.25 MPa, the reaction temperature was controlled at 118° C., and the physical modification treatment was performed for 30 min.

[0075] Furthermore, in step S1, the composite enzyme selected for enzymatic hydrolysis is a combination of bromelain and neutral protease bromelain, and simultaneous enzymatic hydrolysis is adopted. The amount of bromelain added is 0.30 g / 100 g, and the amount of neutral protease bromelain added is 0.6 g / 100 g. The pH is adjusted to 6.0 and the temperature is 60° C. After enzymatic hydrolysis for 4 h, the enzyme is inactivated at 100° C. for 20 min, and the supernatant is obtained by centrifugation.

[0076] Comparative Example 1

[0077] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that no composite ionic liquid is added.

[0078] Comparative Example 2

[0079] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that the composite ionic liquid does not contain soybean polysaccharides.

[0080] Comparative Example 3

[0081] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that the composite ionic liquid does not contain sodium lauryl sulfate.

[0082] Comparative Example 4

[0083] This embodiment provides a preparation method of pearl peptides that is the same as that of Example 2, except that the composite ionic liquid does not contain aspartic acid or glutamic acid.

[0084] Experimental effect test

[0085] The pearl peptides prepared in Example 2 were subjected to reductive alkylation treatment and used as samples. The raw files of mass spectrometry results were obtained by liquid chromatography-mass spectrometry (LC-MS / MS). The total ion current chromatogram ( Figure 1 Mass spectrometry data were analyzed using PEAKS Studio 8.5, identifying high-scoring peptide sequences. Further molecular docking was performed using Autodock Vina software, using tyrosinase (PDB: 2Y9X) as the receptor and a small peptide as the ligand. The resulting whitening active peptide sequences with the lowest binding energy were identified, as shown in the table below. The relative content of each active peptide was calculated using area normalization (measuring the area of ​​each peptide peak and the total chromatographic peak area on the chromatogram, excluding the solvent peak, and calculating the percentage of each peak area to the total peak area); the results are shown in the table below.

[0086]

[0087] The same method was used to calculate the relative content of each active peptide in the pearl peptides prepared in Examples 3-5; the results are shown in the following table.

[0088]

[0089] antioxidant activity;

[0090] The operation was carried out according to the instructions of the prior art kit product (). The ABTS and DPPH free radical scavenging rates were calculated as follows:

[0091]

[0092] Where:

[0093] A0——blank control absorbance value;

[0094] A1——absorbance value after the sample reacts with ABTS (DPPH);

[0095] A2——Sample absorbance value.

[0096] The pearl peptides less than 3000 Da prepared in Examples 2-5 and Comparative Examples 1-4 were formulated into 10.0 mg / mL solutions, and their ABTS and DPPH free radical scavenging abilities were tested, respectively. The results are shown in the following table.

[0097]

[0098] Whitening activity (tyrosinase inhibitory activity);

[0099] Tyrosinase inhibitory activity is one of the in vitro activities for whitening activity assessment, which can be used to reflect its ability to inhibit melanin production. Refer to the provisions of T / GDCA 006-2021. Add L-tyrosine solution, 10.0 mg / mL solution of pearl peptides less than 3000 Da prepared in Examples 2-5 and Comparative Examples 1-4, and PBS buffer to a 96-well plate, incubate in a 37°C water bath for 10 min, add 20 μL of tyrosinase solution, mix and react at 37°C for 5 min± 5 s, and then immediately place in a microplate reader for measurement. Replace L-tyrosine with L-dopa and repeat the above operation. The inhibition rate of mono- and diphenolase activity is calculated as follows:

[0100]

[0101] Where:

[0102] Y——tyrosinase activity inhibition rate, %;

[0103] Ad——absorbance of sample reaction well;

[0104] Ac——absorbance of sample background well;

[0105] Ab——average absorbance of solvent reaction wells;

[0106] Aa——average absorbance of solvent background wells.

[0107] The inhibition rate curve was fitted to obtain a regression equation and the IC50 value of the active peptide was calculated and converted into kojic acid equivalent value according to the following formula.

[0108]

[0109] Where:

[0110] D——kojic acid equivalent value, mg / mg;

[0111] C——IC50 value of the test sample, mg / mL;

[0112] C0——IC50 value of kojic acid, mg / mL.

[0113] The inhibition rates of L-tyrosine and L-dopa were tested respectively, and the results are shown in the following table.

[0114]

[0115] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations that come within the meaning and range of equivalents of the claims be embraced therein.

[0116] Furthermore, it should be understood that although this specification describes the embodiments, not every embodiment contains only one independent technical solution. This description is for clarity only. Those skilled in the art should consider the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art. It should be noted that any technical features not described in detail in this invention can be implemented by any existing technology.

Claims

1. A method for preparing pearl peptides with whitening and antioxidant effects, characterized in that: The following steps are involved: S1. After adding distilled water in appropriate proportions to the nacreous layer powder of Pinctada martensii, a composite ionic liquid was added. After high temperature and high pressure treatment, a composite protease was added in appropriate proportions for enzymatic hydrolysis. The enzyme was inactivated and the supernatant was obtained by centrifugation. S2. After decolorization, the supernatant of step S1 is subjected to ultrafiltration to trap small molecule components, and then vacuum concentrated and freeze-dried to obtain small molecule Pinctada martensii pearl peptides; The following peptides were included: NPPMP1: Thr-Phe-Ser-Gly-Asn-Tyr-Pro; NPPMP2: Gly-Gly-Phe-Gly-Asn-Trp; NPPMP3: Ala-Thr-Phe-Asp-Ala-Ile; NPPMP4: Leu-Lys-Gly-His-Glu-Asp-Leu; NPPMP5: His-Ser-Ser-Ala-His-Ser; NPPMP6: Gly-Gly-Ser-Phe-Ser-Val-Arg; The pearl peptide is Pinctada martensii pearl peptide; In step S1, the composite ionic liquid comprises the following components in parts by weight: 39-57 parts soybean polysaccharide, 15-22 parts sodium lauryl sulfate, 18-24 parts aspartic acid, and 11-19 parts glutamic acid; The addition amount of the composite ionic liquid is 23-35% of the mass of the nacre powder; Adding nacreous layer powder of Pinctada martensii to distilled water in a ratio of 1:3-1:5, controlling the treatment pressure at 0.20-0.25 MPa and the reaction temperature at 115-121°C, and performing physical modification treatment for 20-30 minutes; The compound protease selected for enzymatic hydrolysis is a combination of bromelain and neutral protease, and synchronous enzymatic hydrolysis is adopted. The amount of bromelain added is 0.10-0.30g / 100g, and the amount of neutral protease added is 0.2-0.6g / 100g. The pH is adjusted to 6.0-7.0, the temperature is 45-60°C, the enzymatic hydrolysis is carried out for 4-7h, the enzyme is inactivated at 100°C for 20min, and the supernatant is collected by centrifugation. In step S2, activated carbon and diatomaceous earth are used for combined decolorization. After filtration, the filtrate is further filtered using an ultrafiltration membrane to retain components with a molecular weight of less than 3000 Da.

2. Use of the pearl peptide prepared according to the method of claim 1 in preparing cosmetics with whitening and antioxidant effects.

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