Composition for preventing and controlling grassland locust pests

Abstract

The present invention belongs to the technical field of pesticide compounding, and relates to a composition for grassland locust pest control, wherein the effective ingredients include 155-480 parts of green anisopliae spore powder, 130-350 parts of avermectin, and 8-27 parts of root extract of white flowered caltrop; the green anisopliae spore powder is derived from green anisopliae IPPMHBHC-7, and the preservation number of the green anisopliae IPPMHBHC-7 is CGMCC No.15999. After compounding green anisopliae spore powder, avermectin, and root extract of white flowered caltrop, the present invention finds that it performs well in locust control, and shows a synergistic effect. The composition provided by the present invention has high trapping effect and good control effect, and is suitable for controlling grassland locust pests in conjunction with a pastoral locust control method.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding and relates to a composition for preventing and controlling grassland locust pests. Background Art

[0002] Grassland is the largest terrestrial ecosystem in my country. It is an important material basis for the development of animal husbandry and the basic means of production and living for farmers and herdsmen. In recent years, affected by global climate change, irrational use and other factors, insect pests have gradually become one of the most frequent and most harmful biological disasters on grasslands. Among them, the most serious locust plagues are known as the three major natural disasters along with floods and droughts. Grassland pests eat grass in large quantities, seriously damaging grassland vegetation, posing a serious threat to national ecological security, animal husbandry production and the lives of farmers and herdsmen. Specific types of grassland locusts include: white-striped locust, short-winged locust, Japanese diamondback locust, Asian small car locust, etc.

[0003] Metarhizium anisopliae is an important insect pathogenic fungus. The preparation with it as the main biological active ingredient has the advantages of no residue, easy production, long lasting effect, strong pathogenicity, good application effect, and safety to non-target organisms. Since the first use of Metarhizium anisopliae to control Austrian beetles (Anisopliae aus-triaca), Metarhizium anisopliae has been widely used in pest biological control. In grassland pest control work, Metarhizium anisopliae is mainly used for the control of grassland locusts. CN109401981A discloses a scarab beetle Metarhizium IPPMHBHC-7 and its application. The scarab beetle Metarhizium anisopliae IPPMHBHC-7 has a preservation number of CGMCC No. 15999, which has a good median lethal time and insect dead insect rate for Asian car grasshoppers and hairy-footed grasshoppers.

[0004] Avermectins (AVMs) are the most popular insecticides at home and abroad. They are 16-membered macrolide substances. They were first successfully developed by Merck in the United States. They can inhibit invertebrate neurotransmitters and paralyze insects to death. With the increase in the application of chemical pesticides and the increase in the frequency of prevention and control, locusts will inevitably develop resistance to pesticides, resulting in a decrease in the prevention and control effect. Under the premise of continuously strengthening the protection of the ecological environment, various biological pesticides are also used for grassland locust control, which plays an important role in protecting the biodiversity of grassland ecosystems and improving the natural disaster resistance of ecosystems.

[0005] Calotropisprocera (Aiton) WTAiton is a plant of the genus Calotropis in the Asclepiadaceae family. Existing literature has isolated 8 compounds from the roots of Calotropisprocera, a plant of the Asclepiadaceae family. They were identified as 1-O-methyl-guaiacylglyc-erol, ω-hydroxypropioguaiacone, C-Veratroylglycol, Ficusol, (+)-syringaresinol, 9α-hydroxypinoresinol, p-hydroxybenzaldehyde, and niacinamide by spectral analysis. All of the above compounds were isolated from this species for the first time.

[0006] At present, the main methods of locust control are chemical control and biological control. In actual application, a strategy of using multiple control methods together is often adopted. Chemical control methods mainly include: spraying with chemical agents such as kasik, malathion crude oil, and levofloxacin or using poison bait to kill. Biological control mainly includes the following measures: (1) In locust-affected areas, use high-efficiency and low-toxic pesticides and biological pesticides to protect the predatory natural enemies in the locust area. Chickens, ducks, geese and other poultry can also be raised on the hillside for control. (2) Large-scale application of microsporidia. The above methods have certain disadvantages. Chemical control has certain toxicity and will pollute the environment. Although biological control does not cause pollution, it is very difficult to find effective biological pesticides. The practice of raising poultry is not advisable in some grassland areas with harsh natural environments. Large-scale application of microsporidia may kill some beneficial natural enemies of pests and cause ecological imbalance. Therefore, it is necessary to provide a composition for grassland locust pest control, which combines the advantages of chemical control methods and biological control methods, improves the effect of grassland locust pest control, and reduces the mutual influence between chemical control and biological control. Summary of the invention

[0007] The purpose of the present invention is to provide a composition for grassland locust pest control, which combines the advantages of chemical control means and biological control means, improves the control effect of grassland locust pests, and reduces the mutual influence between chemical control and biological control.

[0008] Based on this, the present invention provides a composition for grassland locust pest control to meet this need in the art. The active ingredients include, by weight, 155-480 parts of green anisopliae spore powder, 130-350 parts of avermectin, and 8-27 parts of root extract of white flowered caltrops; the green anisopliae spore powder is derived from green anisopliae IPPMHBHC-7, and the preservation number of green anisopliae IPPMHBHC-7 is CGMCC No.15999.

[0009] It is easy for those skilled in the art to know that in order to improve the efficacy of compound insecticides, avoid the occurrence of drug resistance, and expand the insecticide spectrum, multiple pesticides are often used in combination or in combination. However, due to the unknown internal mechanism of each component, it is difficult to predict whether the insecticidal effect of multiple pesticides used in combination or in combination is synergistic. After in-depth experimental research, the inventor team of this patent has comprehensively evaluated the effects shown in the toxicity test and found that the composition provided by the present invention has excellent control effects on locusts, and is better than the use effect of a single agent.

[0010] In a preferred embodiment, the composition for grassland locust pest control provided by the present invention comprises, by weight, 270-390 parts of Metarhizium spore powder, 215-290 parts of avermectin and 16-20 parts of root extract of Melon gloeospermum.

[0011] In a more preferred embodiment, the composition for grassland locust pest control provided by the present invention comprises 370 parts of Metarhizium anisopliae spore powder, 240 parts of Avermectin and 17 parts of root extract of Melon gloeospermum officinale.

[0012] In a more preferred embodiment, the composition for grassland locust pest control provided by the present invention comprises, by weight, 367 parts of Metarhizium spore powder, 279 parts of Avermectin and 17 parts of root extract of Melon gloeospermum.

[0013] In a more preferred embodiment, the composition for grassland locust pest control provided by the present invention comprises, by weight, 280 parts of Metarhizium spore powder, 220 parts of Avermectin and 19 parts of root extract of Melon gloeospermum.

[0014] Furthermore, in the composition for grassland locust pest control provided by the present invention, the concentration of Metarhizium anisopliae spore powder is 1×10 8 -5×10 8 Pieces / g.

[0015] Furthermore, in the composition for grassland locust pest control provided by the present invention, the root extract of Melon sphaerocarpa is prepared by using supercritical CO 2 Fluid extraction is obtained; the supercritical CO 2 The pressure of fluid extraction was 28 MPa and the temperature was 53 °C.

[0016] In addition, another object of the present invention is to use the composition for grassland locust pest control in locust pest control. Specifically, the grassland locust includes Asian locust. In addition, the composition for grassland locust pest control provided by the present invention is suitable for controlling grassland locust pests in conjunction with a poultry locust control method. Exemplarily, the poultry locust control method is to release chickens and ducks to control locusts in the locust nymph stage in the grassland locust occurrence area, thereby increasing the economic income of herders while preventing and controlling grassland locusts.

[0017] Compared with the prior art, the composition of the present invention has at least the following advantages or beneficial effects:

[0018] The present invention compounded Metarhizium anisopliae spore powder, avermectin and root extract of Glechoma longituba, and found that it performed excellently in locust control, showing a synergistic effect. The composition provided by the present invention has a high trapping effect and a good control effect, and is suitable for controlling grassland locust pests in conjunction with a poultry locust control method. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is described below in conjunction with embodiments; however, the present invention is not limited to the following embodiments.

[0020] The experimental methods and detection methods in the following embodiments are all conventional methods unless otherwise specified; the agents and materials described are all commercially available unless otherwise specified; the indicator data described are all conventional measurement methods unless otherwise specified.

[0021] This example provides an indoor toxicity test of a composition for controlling grassland locust pests.

[0022] Preparation of Metarhizium anisopliae spore powder: Inoculate Metarhizium anisopliae IPPMHBHC-7 (preservation number CGMCC No. 15999) onto PDA solid medium (200 g potato juice, 20 g agar, 20 g sucrose, 5 g yeast powder, add water to 1000 mL, autoclave at 121°C for 25 min), and culture until spores are produced, colonies and spores are observed. The spore concentration is 2.5×10 8 / g of Green Anisopliae spore powder.

[0023] Avermectin stock solution (B 1a >96.5%), from Zhejiang Qianjiang Biochemical Co., Ltd.

[0024] Calotropis procera root extract is prepared from the dried root of Calotropis procera (Aiton) using supercritical CO 2 Fluid extraction, supercritical CO 2 The pressure of fluid extraction was 28 MPa and the temperature was 53 °C.

[0025] Single-dose group 1: spore concentration was 2.5×10 8 / g of Metarhizium anisopliae spore powder;

[0026] Single-dose group 2: avermectin;

[0027] Single-dose group 3: extract from the root of Melon edulis;

[0028] Test group 1: 155 parts of Metarhizium anisopliae spore powder, 130 parts of avermectin and 8 parts of root extract of Melon oleraceus;

[0029] Test group 2: 270 parts of Metarhizium anisopliae spore powder, 215 parts of avermectin and 16 parts of root extract of Melon oleraceus;

[0030] Test group 3: 370 parts of Metarhizium anisopliae spore powder, 240 parts of avermectin and 17 parts of root extract of Melon oleifera;

[0031] Test group 4: 367 parts of Metarhizium anisopliae spore powder, 279 parts of avermectin and 17 parts of Melon oleraceus root extract;

[0032] Test group 5: 280 parts of Metarhizium anisopliae spore powder, 220 parts of avermectin and 19 parts of root extract of Melon oleraceus;

[0033] Test group 6: 390 parts of Metarhizium anisopliae spore powder, 290 parts of avermectin and 20 parts of root extract of Melon oleifera;

[0034] Test group 7: 480 parts of Metarhizium anisopliae spore powder, 350 parts of avermectin and 27 parts of Melon globulus root extract.

[0035] The stomach poison method was used to determine the toxic effects of the above 10 test agents on the Asian car grasshopper. Asian car grasshopper (Oedaleus decorus asiaticus B. Bienko): 2-3 instar locust nymphs, collected from the Inner Mongolia grassland by the sweeping net method. The active ingredient in each test group was diluted to an appropriate concentration with sterile water. A total of 6 test concentrations were set up, with 20 3-instar Asian car grasshoppers in each concentration, 3 repetitions, and sterile water for the blank control. After oral treatment, the locusts were placed in a special gauze cage and inspected after 48 hours. The number of dead insects was counted and statistically analyzed. The statistical analysis software SPSS 22.0 was used to obtain the LC by the corrected mortality value method. 50 And co-toxicity coefficient. When the co-toxicity coefficient (CTC) is greater than 120, it indicates that the active substance has a synergistic effect, the co-toxicity coefficient (CTC) is between 80 and 120, indicating that the active substance has an additive effect, and the co-toxicity coefficient (CTC) is less than 80, indicating that the active substance has an antagonistic effect. The test results are shown in Table 1.

[0036] Table 1. Co-toxicity coefficients of compositions for grassland locust pest control

[0037]

[0038] As shown in Table 1, after the compounding of Metarhizium anisopliae spore powder, Avermectin and the root extract of Gastrodia elata provided by the present invention, it was found that it performed well in locust control and showed a synergistic effect. Among them, the co-toxicity coefficient was 124.91-157.88, indicating that the effective substances Metarhizium anisopliae spore powder, Avermectin and the root extract of Gastrodia elata have a synergistic effect in locust control after compounding.

[0039] As described above, the present invention can be well implemented. The above embodiments are only descriptions of the preferred implementation modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various changes and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the present invention.

Claims

1. The use of the root extract of Melon oleraceus in enhancing the spore powder of Metarhizium anisopliae and Avermectin for the prevention and control of grassland locust pests, characterized in that: The active ingredients for controlling grassland locust pests are composed of 155-480 parts of Metarhizium anisopliae spore powder, 130-350 parts of Avermectin and 8-27 parts of root extract of Melon oleraceus. The Metarhizium anisopliae spore powder is derived from Metarhizium anisopliae IPPMHBHC-7, and the preservation number of Metarhizium anisopliae IPPMHBHC-7 is CGMCC No.15999; The concentration of the green anisopliae spore powder is 1×10 8 -5×10 8 pcs / g; The root extract of the white flowered caltrop is obtained by extracting the dried root of the white flowered caltrop using supercritical CO2 fluid; the supercritical CO2 fluid extraction pressure is 28MPa and the temperature is 53°C; The LC of the root extract of Melon oleraceus to Cibotium asiatica 50 1709.36mg / L; The grassland locust is the Asian locust.

2. The use according to claim 1, characterized in that: Calculated by weight, the effective ingredients include 270-390 parts of Metarhizium anisopliae spore powder, 215-290 parts of avermectin and 16-20 parts of root extract of Melon oleifera.

3. The use according to claim 2, characterized in that: Calculated by mass, the effective ingredients include 370 parts of Metarhizium anisopliae spore powder, 240 parts of avermectin and 17 parts of root extract of Melon gloeospermum officinale.

4. The use according to claim 2, characterized in that: Calculated by mass, the effective ingredients include 367 parts of Metarhizium anisopliae spore powder, 279 parts of avermectin and 17 parts of root extract of Melon gloeospermum.

5. The use according to claim 2, characterized in that: Calculated by weight, the effective ingredients include 280 parts of Metarhizium anisopliae spore powder, 220 parts of avermectin and 19 parts of root extract of Melon gloeospermum.

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