Bone degreasing method

Abstract

The present invention discloses a bone degreasing method, which relates to the field of oil and fat processing technology. Specifically, it comprises the following steps: S1: placing a human bone in a mixed solution of a degreasing agent and lipase; S2: outputting ultrasonic waves of a preset frequency to the mixed solution while simultaneously controlling the temperature of the mixed solution within a preset temperature range for a predetermined time period, and counting the number of times the predetermined time period has been completed; S3: determining whether the number of times the predetermined time period has been completed is greater than three; if not, repeating steps S1 and S2. The method aims to degrease human bone specimens without polluting the environment or harming the operator.

Description

Technical Field

[0001] The present invention relates to the technical field of grease treatment, in particular to a bone degreasing method. Background Art

[0002] When preparing human skeletal specimens, the most important step is to remove fat from the bones. Traditional methods require the use of organic solvents such as acetone, ether, xylene, and gasoline during this process. These solvents are toxic, harmful, flammable, explosive, and easily used to make drugs. They are state-controlled chemicals and are difficult to procure. Their use not only pollutes the environment but also poses significant health risks to the operator.

[0003] Therefore, how to achieve degreasing of human bone specimens without polluting the environment and damaging the operator's body has become a technical problem that needs to be solved urgently. Summary of the Invention

[0004] The main purpose of the present invention is to provide a bone degreasing method, which aims to achieve degreasing of human bone specimens without polluting the environment or damaging the operator's body.

[0005] In order to achieve the above object, the present invention provides a degreasing method, comprising the following steps:

[0006] S1: placing human bones in a mixed solution of degreasing agent and lipase;

[0007] S2: Outputting ultrasonic waves of a preset frequency to the mixed solution, while controlling the temperature of the mixed solution within a preset temperature range for a predetermined time and counting the number of times the predetermined time is completed;

[0008] S3: Determine whether the number of times the predetermined time is completed is greater than 3 times. If not, repeat steps S1 and S2.

[0009] In one embodiment of the present application, in the mixed solution: the degreasing agent is a neutral degreasing agent with a concentration of 5% to 10%; and the weight ratio of the lipase to the volume of the solution is 1 g / L to 2 g / L.

[0010] In one embodiment of the present application, the concentration of the degreasing agent is a 10% neutral degreasing agent, and the weight ratio of the lipase to the volume of the solution is 2 g / L; or

[0011] The concentration of the degreasing agent is 5% neutral degreasing agent, and the weight ratio of the lipase to the volume of the solution is 1 g / L.

[0012] In one embodiment of the present application, the preset frequency is 40KHZ.

[0013] In one embodiment of the present application, the preset temperature of the mixed solution is 40° or 60°.

[0014] In one embodiment of the present application, the method further includes before step S2:

[0015] Obtain the maximum height of the human skeleton after placement and the maximum height of the mixed solution;

[0016] Determine whether the difference between the maximum height of the mixed solution and the maximum height of the human skeleton after placement meets the preset height; if not, continue to add the mixed solution.

[0017] In one embodiment of the present application, the preset height is 10 cm.

[0018] In one embodiment of the present application, the predetermined time is 240 hours.

[0019] In one embodiment of the present application, it also includes judging whether the degreasing time reaches half of the predetermined time. If so, the human bones are taken out and placed in a dryer for drying, and then placed in a mixed solution of the original degreasing agent and lipase for further degreasing.

[0020] In one embodiment of the present application, a plurality of 0.2 mm openings are also opened on the human skeleton.

[0021] By adopting the above technical solution, the fat on the human bones is removed by placing them in a mixed solution of a degreasing agent and lipase, which will not affect the health of the operator and will not pollute the environment. It has the advantage of easy availability of degreasing materials, and at the same time outputs ultrasonic waves of a preset frequency to the mixed solution, and controls the temperature of the mixed solution within a preset range, thereby accelerating the degreasing work of the human bones to ensure the rapid degreasing of the human bones, effectively improving the degreasing efficiency of the degreasing agent and lipase, and the process is simple and easy to implement. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention will be described in detail below with reference to specific embodiments and accompanying drawings, wherein:

[0023] Figure 1 This is a schematic diagram of the process structure of the first embodiment of the present invention. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the following specific embodiments are only used to explain the present invention and do not constitute a limitation of the present invention.

[0025] like Figure 1 As shown, in order to achieve the above purpose, the present invention provides a degreasing method, comprising the following steps:

[0026] S1: placing human bones in a mixed solution of degreasing agent and lipase;

[0027] S2: Outputting ultrasonic waves of a preset frequency to the mixed solution, while controlling the temperature of the mixed solution within a preset temperature range for a predetermined time and counting the number of times the predetermined time is completed;

[0028] S3: Determine whether the number of times the predetermined time is completed is greater than 3 times. If not, repeat steps S1 and S2.

[0029] Specifically, a degreasing method comprises the following steps:

[0030] Step S1: placing a human skeleton in a mixed solution of a degreasing agent and lipase, and removing fat from the human skeleton through the interaction of the degreasing agent and lipase. Degreasing agents include alkaline degreasing agents, emulsion degreasing agents, and solvent degreasing agents, all of which are commonly used degreasing agents in the prior art. The degreasing agent in this application is preferably an emulsion degreasing agent, also known as a neutral degreasing agent.

[0031] Lipase belongs to the carboxyl ester hydrolase class and can gradually hydrolyze triglycerides into glycerol and fatty acids. Lipase is present in fat-containing tissues of animals, plants, and microorganisms (such as molds and bacteria).

[0032] The interaction between the degreasing agent and lipase can accelerate the removal of fat on human bones. It is a non-toxic, harmless, non-flammable and non-explosive drug and will not cause any harm to the operator's body.

[0033] Step S2: Output ultrasonic waves of a preset frequency to the mixed solution. By outputting ultrasonic waves to the mixed solution, the fat on the human bones that has preferentially come into contact with the degreasing agent and lipase in the mixed solution can be quickly detached, and the fat on the human bones that has not come into contact with the degreasing agent and lipase in the mixed solution can be quickly contacted with the mixed solution, thereby improving the degreasing efficiency of the human bones.

[0034] At the same time, the temperature of the mixed solution is controlled within a preset range, for example, at 40°C, 30°C, 35°C, and so on. The temperature of the mixed solution is controlled within a preset range, which is related to the temperature tolerance of the lipase, to ensure that the lipase is at its maximum activity. In this application, the temperature of the mixed solution is controlled at 40°C. This is maintained for a predetermined time, and the number of times the predetermined time is completed is counted.

[0035] The following are examples:

[0036] First, place the human skeleton in a cleaning machine, add 10% degreasing agent and 2g / L lipase, adjust the sonic frequency to 40kHz, and the temperature to 60°C. Cover the machine and observe the state of the oil in the mixed solution after 48 hours. The number of times the 48-hour period has been completed is also counted.

[0037] Determine whether the number of times the 48-hour cycle has been completed is greater than three. If so, the human skeletal fat has been completely defatted and can be washed with clean water. If the number of times the 48-hour cycle has been completed is less than three, the human skeletal fat has not been completely defatted, and steps s1 and s2 need to be repeated to completely defatt the human skeletal fat.

[0038] First, place the human skeleton in a cleaning machine, add 5% degreasing agent and 1g / L lipase, adjust the sonic frequency to 40kHz, and the temperature to 40°C. Cover the machine and observe the state of the oil in the mixed solution after 48 hours. Count the number of times the 48-hour period has been completed.

[0039] Determine whether the number of times the 48-hour cycle has been completed is greater than three. If so, the human skeletal fat has been completely defatted and can be washed with clean water. If the number of times the 48-hour cycle has been completed is less than three, the human skeletal fat has not been completely defatted, and steps s1 and s2 need to be repeated to completely defatt the human skeletal fat.

[0040] By adopting the above technical solution, the fat on the human bones is removed by placing them in a mixed solution of a degreasing agent and lipase, which will not affect the health of the operator and will not pollute the environment. It has the advantage of easy availability of degreasing materials, and at the same time outputs ultrasonic waves of a preset frequency to the mixed solution, and controls the temperature of the mixed solution within a preset range, thereby accelerating the degreasing work of the human bones to ensure the rapid degreasing of the human bones, effectively improving the degreasing efficiency of the degreasing agent and lipase, and the process is simple and easy to implement.

[0041] In one embodiment of the present application, in the mixed solution: the degreasing agent is a neutral degreasing agent with a concentration of 5% to 10%; and the weight ratio of the lipase to the volume of the solution is 1 g / L to 2 g / L.

[0042] Specifically, the concentration of the degreaser in the mixed solution is 5% to 10%, and the degreaser is a neutral degreaser. Using a neutral degreaser can effectively prevent the degreaser from harming the operator's body. It is well known that when the concentration of the degreaser is low, the degreasing speed of human bones will be reduced; when the concentration of the degreaser is high, the viscosity of the corresponding degreaser will increase. At this time, the human bones placed in the mixed solution and the high-concentration degreaser will adhere more tightly to the human bones. At this time, the fat on the human bones is not easy to separate from the human bones, which reduces the efficiency of fat removal from the human bones. Setting the concentration of the degreaser to 5% to 10% can effectively avoid the defect of difficulty in removing fat from human bones due to excessively high degreaser concentrations, while also achieving efficient fat removal from human bones.

[0043] The weight ratio of lipase to the volume of the solution is: 1g / L to 2g / L.

[0044] When the weight ratio of lipase to solution volume is 1g / L, the corresponding degreasing agent concentration is 5%. Using this combination, while completing human bone degreasing, the amount of lipase and degreasing agent used can be reduced, reducing production costs and also achieving an optimal ratio of degreasing agent to lipase. When the weight ratio of lipase to solution volume is 2g / L, the corresponding degreasing agent concentration is 10%. Using this combination, while completing human bone degreasing, the degreasing efficiency of human bone degreasing can be accelerated and the degreasing time can be reduced.

[0045] In one embodiment of the present application, the concentration of the degreasing agent is a 10% neutral degreasing agent, and the weight ratio of the lipase to the volume of the solution is 2 g / L; or

[0046] The concentration of the degreasing agent is 5% neutral degreasing agent, and the weight ratio of the lipase to the volume of the solution is 1 g / L.

[0047] In one embodiment of the present application, the preset frequency is 40KHZ.

[0048] Specifically, the preset frequency is 40KHZ, and setting the output frequency of the ultrasound to 400KHZ can effectively improve the reaction efficiency of the degreasing agent and lipase with human bones.

[0049] Under the premise that the number of human bones is 1, the degreasing agent concentration is 5%, the temperature is 40, and the lipase is 1g / L, the effects of different ultrasonic lengths on the reaction are shown in the following table. The specific performance is as follows:

[0050]

[0051] Under the same conditions as the number of human bones, the concentration of degreasing agent is 10%, the temperature is 600℃, and the lipase is 2g / L, the effects of different ultrasonic lengths on the reaction are shown in the following table. The specific performance is as follows:

[0052]

[0053]

[0054] In one embodiment of the present application, the preset temperature of the mixed solution is 40° or 60°.

[0055] In one embodiment of the present application, the method further includes before step S2:

[0056] Obtain the maximum height of the human skeleton after placement and the maximum height of the mixed solution;

[0057] Determine whether the difference between the maximum height of the mixed solution and the maximum height of the human skeleton after placement meets the preset height; if not, continue to add the mixed solution.

[0058] Specifically, before step S2, the following steps are also included:

[0059] Obtain the maximum height of the human skeleton after placement. For example, when the human skeleton is placed vertically, the maximum height of the human skeleton after placement is equal to the length of the human skeleton; when the human skeleton is placed horizontally, the maximum height of the skeleton after placement is equal to the vertical height of the human skeleton. Obtain the maximum height of the mixed solution, subtract the maximum height of the human skeleton after placement from the maximum height of the mixed solution, and obtain the difference between the maximum height of the mixed solution and the maximum height of the human skeleton after placement. Determine whether the difference meets the preset height. If the difference meets the preset height, it indicates that the total amount of the current mixed solution meets the condition. If the difference does not meet the preset height, it indicates that the total amount of the current mixed solution is insufficient and more mixed solution needs to be added to the container.

[0060] By ensuring that the mixed solution always meets the conditions, it is avoided that the mixed solution gradually decreases during the reaction process, resulting in partial exposure of human bones and uneven degreasing.

[0061] The process is simple and easy to implement.

[0062] In one embodiment of the present application, the preset height is 10 cm.

[0063] In one embodiment of the present application, the predetermined time is 240 hours.

[0064] In one embodiment of the present application, it also includes judging whether the degreasing time reaches half of the predetermined time. If so, the human bones are taken out and placed in a dryer for drying, and then placed in a mixed solution of the original degreasing agent and lipase for further degreasing.

[0065] Specifically, the time the human skeleton has been placed in the mixed solution is obtained, and it is determined whether the time the human skeleton has been placed in the mixed solution is equal to half of the predetermined time. If it is equal to half of the predetermined time, the skeleton is removed from the mixed solution and placed in a dryer for drying. After the dryer dries the human skeleton, the skeleton is placed back into the original mixed solution to continue degreasing the skeleton. During the degreasing process, the skeleton is removed from the mixed solution and dried, so that the mixed solution adhered to the skeleton is separated from the surface of the skeleton. This avoids the situation in which the contact area between the mixed solution and the skeleton is reduced due to dissolved fat adhering to the skeleton during the subsequent degreasing process, thereby further improving the degreasing efficiency.

[0066] In one embodiment of the present application, a plurality of 0.2 mm openings are also opened on the human skeleton.

[0067] Specifically, it also includes opening multiple 0.2mm openings on the human skeleton. The multiple in this application refers to two or more. The number of openings in this application is preferably 5 to 10 holes. Setting 5 to 10 holes facilitates the mixed solution to enter the human skeleton, thereby completing the separation of fat inside the human skeleton. The diameter of the opening is set to 0.2mm to avoid the destruction of the shape of the human skeleton due to the opening being too large.

[0068] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention description and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A degreasing method, characterized in that: The following steps are involved: S1: Multiple 0.2 mm openings are made on the human bone, and the human bone is placed in a mixed solution of a degreasing agent and lipase; S2: Outputting ultrasonic waves of a preset frequency to the mixed solution while controlling the temperature of the mixed solution within a preset temperature range for a predetermined time, determining whether the degreasing time has reached half of the predetermined time, and if so, removing the human bones, placing them in a dryer for drying, and then placing them again in the mixed solution of the original degreasing agent and lipase to continue degreasing; and counting the number of times the predetermined time has been completed; S3: Determine whether the number of times the predetermined time is completed is greater than 3 times. If not, repeat steps S1 and S2; In the mixed solution, the degreasing agent is a neutral degreasing agent, and the concentration of the degreasing agent in the mixed solution is 5% to 10%; the weight ratio of the lipase to the volume of the solution is 1g / L to 2g / L.

2. The degreasing method according to claim 1, wherein The degreasing agent is a neutral degreasing agent, the concentration of the degreasing agent in the mixed solution is 10%, and the weight ratio of the lipase to the volume of the solution is 2 g / L; or The degreasing agent is a neutral degreasing agent, the concentration of the degreasing agent in the mixed solution is 5%, and the weight ratio of the lipase to the volume of the solution is 1 g / L.

3. The degreasing method according to claim 1, wherein The preset frequency is 40 kHz.

4. The degreasing method according to claim 1, wherein The preset temperature of the mixed solution is 40°C or 60°C.

5. The degreasing method according to claim 1, wherein Before step S2, the method further includes: Obtain the maximum height of the human skeleton after placement and the maximum height of the mixed solution; Determine whether the difference between the maximum height of the mixed solution and the maximum height of the human skeleton after placement meets the preset height; if not, continue to add the mixed solution.

6. The degreasing method according to claim 5, wherein The preset height is 10 cm.

7. The degreasing method according to claim 1, wherein The predetermined time is 240 hours.

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