Application of barley grass exosomes in the preparation of products with anti-aging and repair effects

Abstract

This invention discloses the use of barley grass exosomes in the preparation of products with anti-aging and restorative effects, relating to the field of biotechnology. Barley grass exosomes can increase cell survival under oxidative stress, promote cell proliferation, enhance cellular antioxidant capacity, reduce MDA levels, and increase SOD levels. They can also enhance cellular collagen synthesis and promote the secretion of type I and type III collagen. In terms of anti-aging and restorative effects, barley grass exosomes and adipose-derived mesenchymal stem cell exosomes exhibit a certain synergistic effect.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to the use of barley grass exosomes in preparing products with anti-aging and repairing effects. Background Art

[0002] Exosomes are extracellular vesicles secreted by cells. Plant exosomes have a diameter of approximately 40 to 150 nm and are morphologically similar to animal exosomes, with a phospholipid bilayer structure and a saucer or cup shape. They contain various plant chemical components and a large amount of lipids, microRNA (miRNA), DNA, and proteins. The active substances contained in exosomes (particularly miRNA) and their transmission are key to maintaining intercellular communication. Numerous studies have demonstrated that plant exosomes can facilitate the transfer of substances and information from plants to animals across species.

[0003] Skin aging is a complex physiological process, with peroxidative damage being a key contributor to both natural and photoaging. Fibroblasts are the most important cellular component of the dermis, and their decreased number, morphological changes, and weakened or declining secretory and synthetic functions are closely associated with skin aging. Studies have shown that peroxidative damage to skin fibroblast function can lead to changes in the cell cycle, apoptosis, and abnormal collagen metabolism, resulting in dry, rough skin, deeper wrinkles, decreased elasticity, and sagging skin. It is crucial to develop a drug that can effectively reduce oxidative damage and promote skin repair. Summary of the Invention

[0004] The present invention aims to address at least one of the technical problems existing in the prior art. To this end, the present invention proposes the use of barley grass exosomes in the preparation of products with anti-aging and repairing effects. Barley grass exosomes can effectively enhance the antioxidant capacity of cells, reduce oxidative damage, promote cell proliferation, and enhance the ability of skin fibroblasts to secrete collagen.

[0005] The present invention also provides an anti-aging repair composition.

[0006] The present invention also provides applications of the anti-aging repair composition.

[0007] The present invention also provides a product comprising the anti-aging repair composition.

[0008] According to the first embodiment of the present invention, the use of barley grass exosomes in the preparation of products with anti-aging and repair effects.

[0009] The anti-aging repair composition according to the embodiment of the present invention has at least the following beneficial effects:

[0010] The present invention found that barley grass exosomes can improve the survival rate of cells under oxidative stress, promote cell proliferation, improve the antioxidant capacity of cells, reduce MDA levels, and increase SOD levels; and can also improve the collagen synthesis ability of cells and promote the secretion of type I collagen and type III collagen. Moreover, barley grass exosomes do not have the problem of high effective dose, difficulty in ensuring safety, and susceptibility to blood-brain barrier obstruction. The active ingredients in barley grass exosomes are in a natural soluble form, which solves the problem that many active ingredients are insoluble in water and have low physiological availability; and barley grass exosomes have extremely strong stability. In addition to containing effective chemical ingredients, they also contain a variety of active ingredients and have a natural composite effect; the diameter is less than 150nm, and they have good penetrability to various physiological barriers (such as the blood-brain barrier and the skin barrier).

[0011] According to some embodiments of the present invention, the product is a cosmetic, a medicine, or a kit.

[0012] According to some embodiments of the present invention, the product further comprises a cosmetically, pharmaceutically, chemically or biologically acceptable carrier.

[0013] An anti-aging repair composition according to a second embodiment of the present invention includes barley seedling exosomes and adipose-derived mesenchymal stem cell exosomes.

[0014] The anti-aging repair composition according to the embodiment of the present invention has at least the following beneficial effects:

[0015] The anti-aging repair composition of the embodiment can promote cell proliferation, reduce cell oxidative damage, and improve the cell's antioxidant capacity and collagen secretion capacity.

[0016] By utilizing the synergistic effect between barley seedling exosomes and adipose-derived mesenchymal stem cell exosomes, the problem of low acquisition rate of adipose-derived mesenchymal stem cell exosomes due to insufficient stem cell sources (especially stem cell sources from human living tissues) can be effectively avoided, thereby effectively reducing the amount of adipose-derived mesenchymal stem cell exosomes used and achieving better results.

[0017] According to some embodiments of the present invention, the composition comprises, by weight, 0.5-7 parts of barley grass exosomes and 0.5-7 parts of adipose-derived mesenchymal stem cell exosomes. For example, the composition may comprise 0.5, 1, 2, 3, 4, 5, 6, or 7 parts of barley grass exosomes and 0.5, 1, 2, 3, 4, 5, 6, or 7 parts of adipose-derived mesenchymal stem cell exosomes.

[0018] According to some embodiments of the present invention, the effects of the anti-aging repair composition include at least one of improving the antioxidant capacity of cells and promoting the secretion and production of cellular collagen.

[0019] According to some embodiments of the present invention, promoting the secretion and production of cellular collagen includes promoting the secretion and production of type I collagen and / or type III collagen.

[0020] According to some embodiments of the present invention, improving the antioxidant capacity of cells includes at least one of reducing the MDA level and increasing the SOD level.

[0021] According to some embodiments of the present invention, the adipose-derived mesenchymal stem cell exosomes include human adipose-derived mesenchymal stem cell exosomes.

[0022] According to some embodiments of the present invention, the anti-aging repair composition can enhance cellular antioxidant capacity, and the anti-aging repair composition comprises 1 part barley grass exosomes and 1 part adipose-derived mesenchymal stem cell exosomes; or 1 part barley grass exosomes and 5 parts adipose-derived mesenchymal stem cell exosomes. Preferably, the effective concentration of the anti-aging repair composition is 1 μg / mL barley grass exosomes and 1 μg / mL adipose-derived mesenchymal stem cell exosomes; or 1 μg / mL barley grass exosomes and 5 μg / mL adipose-derived mesenchymal stem cell exosomes.

[0023] According to some embodiments of the present invention, the anti-aging repair composition can enhance the collagen secretion capacity of cells, and the anti-aging repair composition comprises 1 part barley grass exosomes and 5 parts adipose-derived mesenchymal stem cell exosomes; or 5 parts barley grass exosomes and 5 parts adipose-derived mesenchymal stem cell exosomes. Preferably, the effective concentration of the anti-aging repair composition is 1 μg / mL barley grass exosomes and 5 μg / mL adipose-derived mesenchymal stem cell exosomes; or 5 μg / mL barley grass exosomes and 5 μg / mL adipose-derived mesenchymal stem cell exosomes.

[0024] According to some embodiments of the present invention, the method for preparing barley grass exosomes comprises the following steps: squeezing barley grass juice and collecting exosomes from the supernatant.

[0025] According to some embodiments of the present invention, a buffer solution is further added when the barley grass juice is squeezed. The buffer solution is isotonic with the physiological environment and includes at least one of physiological saline, phosphate buffer, and Hank's buffer.

[0026] According to some embodiments of the present invention, the method for preparing exosomes from adipose-derived mesenchymal stem cells comprises the following steps: culturing the adipose-derived mesenchymal stem cells, and collecting exosomes from the cultured adipose-derived mesenchymal stem cells or the supernatant of their culture fluid.

[0027] According to some embodiments of the present invention, the method for collecting exosomes comprises at least one of density gradient centrifugation, size exclusion chromatography, differential centrifugation, polyethylene glycol precipitation, immunoseparation, and screening separation.

[0028] According to some embodiments of the present invention, a method for collecting exosomes includes differential centrifugation. The differential centrifugation method comprises the following steps: centrifugation at 200-400g for 5-20 minutes at 0-4°C to collect a first supernatant; centrifugation at 1500-2500g for 10-30 minutes at 0-4°C to collect a second supernatant; centrifugation at 10,000-14,000g for 25-45 minutes at 0-4°C to collect a third supernatant; centrifugation at 10,000-14,000g for 25-45 minutes at 0-4°C to collect a fourth supernatant; and centrifugation at 130,000-170,000g for 70-90 minutes at 0-4°C, discarding the supernatant, and obtaining exosomes.

[0029] The use of the anti-aging repair composition according to the third embodiment of the present invention in preparing a product with anti-aging repair effects. Since all the technical solutions of the anti-aging repair composition of the above embodiment are adopted, it has at least all the beneficial effects brought by the technical solutions of the above embodiment.

[0030] According to some embodiments of the present invention, the anti-aging and repairing effects include at least one of improving the antioxidant capacity of cells and promoting the secretion and production of cellular collagen.

[0031] According to some embodiments of the present invention, promoting the secretion and production of cellular collagen includes promoting the secretion and production of type I collagen and / or type III collagen.

[0032] According to some embodiments of the present invention, improving the antioxidant capacity of cells includes at least one of reducing the MDA level and increasing the SOD level.

[0033] According to a fourth embodiment of the present invention, a product having anti-aging and repairing effects includes the anti-aging and repairing combination described in the second embodiment. Because the product utilizes all the technical solutions of the anti-aging and repairing combination of the above embodiment, it has at least all the beneficial effects brought about by the technical solutions of the above embodiment.

[0034] According to some embodiments of the present invention, the product is a cosmetic, a medicine, or a kit.

[0035] According to some embodiments of the present invention, the product further comprises a cosmetically, pharmaceutically, chemically or biologically acceptable carrier.

[0036] According to some embodiments of the present invention, the dosage form of the product includes at least one of a gel, an emulsion, a solution, a jelly, an aerosol, a powder, a granule, a capsule, and a suspension.

[0037] According to some embodiments of the present invention, the anti-aging repair composition accounts for 1 to 99% of the total weight of the product.

[0038] Other features and advantages of the present invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 These are TEM images of the exosomes prepared in Examples 1 to 4 of the present invention. DETAILED DESCRIPTION

[0040] The following will clearly and completely describe the concept and technical effects of the present invention in conjunction with the embodiments to fully understand the purpose, features and effects of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0041] If the specific conditions are not specified in the examples, the experiments were carried out under conventional conditions or those recommended by the manufacturer. All reagents or instruments used, if the manufacturer is not specified, are commercially available conventional products.

[0042] In the description of the present invention, if there are descriptions of first, second, third, fourth, etc., they are only used to distinguish the technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0043] In the description of the present invention, the terms "comprises" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or apparatus that includes a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to the process, method, product or apparatus.

[0044] The complete culture medium used in the examples was DMEM / F12 medium containing 10% FBS and 1% double antibody.

[0045] Example 1

[0046] This embodiment provides a method for preparing barley grass exosomes, the steps of which are as follows:

[0047] Fresh barley grass was cut into small pieces and ground into a manual juicer to obtain barley grass juice, and the juice was collected; the juice was centrifuged at 4°C, 300g for 10 min, and the first supernatant was collected; the juice was centrifuged at 4°C, 2000g for 20 min, and the second supernatant was collected; the juice was centrifuged at 4°C, 12000g for 35 min, and the third supernatant was collected, and the process was repeated once; the juice was centrifuged at 4°C, 150000g for 80 min, the supernatant was discarded, and PBS was added to resuspend the precipitate to obtain a solution containing barley grass exosomes.

[0048] Example 2

[0049] This embodiment provides a method for preparing barley grass exosomes, and the steps are as follows:

[0050] Fresh barley grass was cut into small pieces, added with 5 times its weight of PBS, placed in a magnetic stirrer, and stirred at 600 rpm for 60 minutes to obtain barley grass juice, and the juice was collected; the juice was centrifuged at 4°C, 300g for 10 minutes, and the first supernatant was collected; the juice was centrifuged at 4°C, 2000g for 20 minutes, and the second supernatant was collected; the juice was centrifuged at 4°C, 12000g for 35 minutes, and the third supernatant was collected, and the process was repeated; the juice was centrifuged at 4°C, 150000g for 80 minutes, the supernatant was discarded, and PBS was added to resuspend the precipitate to obtain a solution containing barley grass exosomes.

[0051] Example 3

[0052] This embodiment provides a method for preparing barley grass exosomes, and the steps are as follows:

[0053] Fresh barley grass was cut into small pieces, 5 times its weight of PBS was added and juiced in a food processor to obtain barley grass juice, and the juice was collected; the juice was centrifuged at 4°C, 300g for 10 minutes, and the first supernatant was collected; the juice was centrifuged at 4°C, 2000g for 20 minutes, and the second supernatant was collected; the juice was centrifuged at 4°C, 12000g for 35 minutes, and the third supernatant was collected, and the process was repeated; the juice was centrifuged at 4°C, 150000g for 80 minutes, the supernatant was discarded, and PBS was added to resuspend the precipitate to obtain a solution containing barley grass exosomes.

[0054] Example 4

[0055] This embodiment provides a method for preparing exosomes of human adipose-derived mesenchymal stem cells (hADSCs), comprising the following steps:

[0056] Human adipose-derived mesenchymal stem cells were seeded into culture flasks. When the cells grew to 80-90%, the culture medium was removed, the cells were washed twice with PBS, and DMEM / F12 culture medium without exosomes was added. The cells were cultured for another 48 hours and the supernatant was collected. The supernatant was centrifuged at 300g at 4°C for 10 minutes to collect the first supernatant; the second supernatant was centrifuged at 2000g at 4°C for 20 minutes to collect the second supernatant; the third supernatant was centrifuged at 12000g at 4°C for 35 minutes to collect the third supernatant, twice; the supernatant was centrifuged at 150000g at 4°C for 80 minutes, the supernatant was discarded, and the precipitate was resuspended in PBS to obtain a solution containing hADSCs exosomes.

[0057] Example 5

[0058] This embodiment provides an anti-aging repair composition composed of 1 μg of barley grass exosomes and 5 μg of hADSCs exosomes.

[0059] Example 6

[0060] This embodiment provides an anti-aging repair composition consisting of 5 μg of barley grass exosomes and 5 μg of hADSCs exosomes.

[0061] Example 7

[0062] This embodiment provides an anti-aging repair composition consisting of 1 μg of barley grass exosomes and 1 μg of hADSCs exosomes.

[0063] Example 8

[0064] This embodiment provides an anti-aging repair composition composed of 5 μg of barley grass exosomes and 1 μg of hADSCs exosomes.

[0065] Example 9

[0066] This embodiment provides an anti-aging repair composition, which is 1 μg of barley grass exosomes.

[0067] Example 10

[0068] This embodiment provides an anti-aging repair composition, which is 5 μg of barley grass exosomes.

[0069] Example 11

[0070] This embodiment provides an anti-aging repair composition, which is 10 μg of barley grass exosomes.

[0071] Comparative Example 1

[0072] This comparative example provides an anti-aging repair composition, which is 1 μg of hADSCs exosomes.

[0073] Comparative Example 2

[0074] This comparative example provides an anti-aging repair composition, which is 5 μg of hADSCs exosomes.

[0075] Comparative Example 3

[0076] This comparative example provides an anti-aging repair composition, which is 10 μg of hADSCs exosomes.

[0077] Comparative Example 4

[0078] This comparative example provides an anti-aging repair composition, which is a barley grass extract, and the extraction method is as follows:

[0079] A certain amount of barley grass was weighed, crushed, added with 10 times the mass of distilled water, soaked for 60 minutes, filtered to remove impurities, and the filtrate was concentrated under vacuum to obtain the barley grass extract.

[0080] Test Example 1

[0081] This test example detected the morphology of the barley grass exosomes prepared in Examples 1 to 3 and the hADSCs exosomes prepared in Example 4. The detection method is as follows:

[0082] Use a 10μL pipette to accurately draw 10μL of exosome solution and drop it onto a 2mm sample-loading copper grid. After standing at room temperature for 3 minutes, use clean filter paper to gently absorb excess liquid from the edge of the sample-loading copper grid. After negative staining with 3% sodium phosphotungstate solution at room temperature for 5 minutes, gently wash it with double distilled water, dry it at room temperature for 2 minutes, and image it on the machine (the operating voltage of the transmission electron microscope is 80kV). Observe and photograph it under a transmission electron microscope.

[0083] Test results such as Figure 1 shown.

[0084] Cup-shaped vesicles were observed in both the barley grass exosome solutions of Examples 1 and 2, but they also contained a large amount of impurities. The barley grass exosome solution of Example 3 had fewer impurities and a better morphology, consistent with the cup-shaped exosome morphology. Cup-shaped vesicles were also observed in the hADSCs exosome solution of Example 4, but were significantly smaller than those in barley grass vesicles.

[0085] Test Example 2

[0086] 1. This test example tests the antioxidant effects of the anti-aging repair compositions of Examples 5 to 11 and Comparative Examples 1 to 4.

[0087] The detection method is as follows:

[0088] (1) Human skin fibroblasts (provided by Beina Bio) were placed in complete culture medium and cultured in a 37°C constant temperature incubator with 5% CO2. Cells in good growth condition were collected by trypsin digestion, 3 mL of complete culture medium was added to each dish, and the cells were pipetted and placed in a 5 mL sterile centrifuge tube. The cells were gently blown to mix them evenly. 20 μL of cell culture medium was pipetted and placed in a 1.5 mL centrifuge tube. An equal volume of trypan blue solution was added and mixed evenly. An appropriate amount was pipetted and placed in a cell counting plate. The cells were counted using an automatic counter to obtain the number of cells. An appropriate amount of cell fluid was pipetted and placed in a sample tank. An appropriate amount of complete culture medium was added and mixed evenly. 100 μL of cell fluid was pipetted using a pipette and placed in a 96-well cell culture plate. The cell density was 6000 cells / well. The cells were evenly distributed using the figure-eight method or the cross method. The cells were placed in an incubator overnight to allow them to adhere to the wall.

[0089] (2) After the cells adhered, the culture medium was discarded, and 600 μM H2O2 and the anti-aging repair composition (the final concentrations of each example or comparative example are shown in Table 1) were added to treat the cells for 24 hours, which were recorded as the experimental group; cells without any treatment were used as the control group; cells treated with only 600 μM H2O2 were used as the model group; and complete culture medium without cells was used as the blank group; each group had 6 replicate wells. After treatment, the culture medium was discarded, and 100 μL CCK-8 solution (CCK-8 stock solution: culture medium = 1:9) was added to the cells and incubated in an incubator for 40 minutes. The absorbance (OD value) of each well was measured using a microplate reader at a detection wavelength of 450 nm.

[0090] Cell viability = (OD value of the experimental group - OD value of the blank group) / (OD value of the control group - OD value of the blank group) × 100%.

[0091] The experimental results are shown in Table 1.

[0092] Table 1

[0093]

[0094]

[0095] Treatment with 5 μg / mL or more of barley seedling exosomes or hADSCs exosomes significantly inhibited oxidative damage caused by 600 μM H2O2 and promoted cell proliferation. Combinations of barley seedling exosomes and hADSCs exosomes at varying concentrations also significantly inhibited oxidative damage caused by 600 μM H2O2 and promoted cell proliferation. 1 μg / mL barley seedling exosomes and 1 μg / mL hADSCs exosomes, as well as 1 μg / mL barley seedling exosomes and 5 μg / mL hADSCs exosomes, exhibited a strong synergistic effect, reducing oxidative damage and promoting cell proliferation.

[0096] 2. SOD and MDA are the main markers for detecting oxidative stress damage. SOD is an important antioxidant enzyme in the body. MDA is a lipid peroxide formed by the attack of polyunsaturated fatty acids by oxygen free radicals. It can reflect the degree of lipid peroxidation in the body and indirectly reflect the degree of cell damage. This test example tested the effects of the anti-aging repair compositions of Examples 5-10 and Comparative Examples 1-2 on superoxide dismutase (SOD) and malondialdehyde (MDA) in human skin fibroblasts.

[0097] The detection method is as follows:

[0098] Cells were seeded in 24-well plates at a density of 3 × 10 4 Each well was treated with different anti-aging repair compositions (final concentrations shown in Table 1) and 600 μM H2O2. 600 μM H2O2 was added to the model group wells for 8 hours. The cell culture medium was discarded and the cells were washed twice with PBS. 200 μL of PBS was added and the cells were scraped off with a cell scraper. The cell fluid was aspirated with a pipette and placed in a 1.5 mL centrifuge tube. Grinding beads were added and then leveled and placed in a ball mill for 300 seconds. Centrifuged at 4°C and 12,000 rpm for 5 minutes, the supernatant was placed in another centrifuge tube, centrifuged again, the supernatant was taken, and mixed to obtain the sample to be tested. The MDA level was detected using the MDA assay kit (WLA048, provided by Shenyang Wanlei Biotechnology Co., Ltd.), and the protein concentration of the sample was determined using the BCA kit. The operation was carried out strictly according to the instructions.

[0099] Cells were seeded in 24-well plates at a density of 3 × 10 4 Cells were cultured overnight to allow attachment. Each well was treated with different anti-aging repair compositions (final concentrations shown in Table 2) and 600 μM H₂O₂. The model group was treated with 600 μM H₂O₂ for 8 hours. The culture medium was discarded and the cells were washed twice with PBS. SOD enzyme activity units were determined using a total SOD activity assay kit (S0101, provided by Shanghai Biyuntian Biotechnology Co., Ltd.), and protein concentration was determined using a BCA assay. All procedures were performed strictly according to the manufacturer's instructions.

[0100] The test results are shown in Table 2.

[0101] Table 2

[0102]

[0103]

[0104] Barley grass exosomes, hADSCs exosomes, or their combination can effectively reduce MDA levels and increase SOD levels. Specifically, 1 μg / mL barley grass exosomes and 5 μg / mL hADSCs exosomes exhibited a significant synergistic effect, effectively enhancing the antioxidant capacity of human skin fibroblasts.

[0105] 3. This test example tests the effects of the anti-aging repair compositions of Examples 5 to 10 and Comparative Examples 1 to 2 on the collagen synthesis ability of human skin fibroblasts.

[0106] The test method is as follows:

[0107] Cells were seeded in 24-well plates at a density of 3 × 10 4 Each well was treated with different anti-aging repair compositions (final concentrations shown in Table 3) and 600 μM H2O2. 600 μM H2O2 was added to the model group wells for 8 hours. The cell culture medium was discarded, and the cells were washed twice with PBS. 500 μL of Trizol was added, and total RNA was extracted and reverse transcribed to obtain cDNA. qPCR was performed using the SYBR Green method, and the relative value of the target gene was calculated according to the 2-ΔΔCt method, where ΔΔCt = (Ct target gene - Ct GAPDH) experimental group - (Ct target gene - Ct GAPDH) control group.

[0108] Among them, the upstream primer used to detect the expression of type I collagen was 5'-TACAGCGTCACTGTCGATGGC-3', and the downstream primer was 3'-TCAATCACTGTCTTGCCCCAG-5'; the upstream primer used to detect the expression of type III collagen was 5'-AATTTGGTGTGGACGTTGGC-3', and the downstream primer was 3'-TTGTCGGTCACTTGCACTGG-5'; the internal reference was GAPDH, and the upstream primer used for detection was 5'-CAACAGCGACACCCACTCCT-3', and the downstream primer was 3'-CACCCTGTTGCTGTAGCCAAA-5'.

[0109] The reaction conditions of qPCR were as follows: 95°C for 2 min; 95°C for 15 s, 60°C for 30 s, and 72°C for 30 s, for 40 cycles.

[0110] The experimental results are shown in Table 3.

[0111] Table 3

[0112]

[0113]

[0114] Barley grass exosomes, hADSCs exosomes, or a combination thereof can effectively promote the synthesis of type I and type III collagen in human skin fibroblasts. A significant synergistic effect was observed between 1 μg / mL barley grass exosomes and 5 μg / mL hADSCs exosomes, as well as between 5 μg / mL barley grass exosomes and 5 μg / mL hADSCs exosomes, effectively increasing the synthesis of type I and type III collagen in human skin fibroblasts and enhancing skin repair effects.

[0115] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the relevant technical field without departing from the scope of the present invention.

Claims

1. An anti-aging repair composition, characterized in that: Including barley grass exosomes and adipose-derived mesenchymal stem cell exosomes.

2. The anti-aging repair composition according to claim 1, characterized in that The composition comprises, by weight, 0.5 to 7 parts of barley grass exosomes and 0.5 to 7 parts of adipose-derived mesenchymal stem cell exosomes.

3. The anti-aging repair composition according to claim 2, characterized in that The method for preparing barley grass exosomes comprises the following steps: squeezing barley grass juice and collecting exosomes from the supernatant.

4. The anti-aging repair composition according to claim 3, characterized in that The method for collecting exosomes includes at least one of density gradient centrifugation, size exclusion chromatography, differential centrifugation, polyethylene glycol precipitation, immunoseparation, and screening separation.

5. Use of the anti-aging and repairing composition according to any one of claims 1 to 4 in the preparation of products with anti-aging and repairing effects.

6. The use according to claim 5, characterized in that The anti-aging and repairing effects include at least one of improving the antioxidant capacity of cells and promoting the secretion and generation of cell collagen.

7. The use according to claim 6, characterized in that Promoting the secretion and production of cellular collagen includes promoting the secretion and production of type I collagen and / or type III collagen.

8. The use according to claim 6, characterized in that Improving the antioxidant capacity of cells includes at least one of reducing the MDA level and increasing the SOD level.

9. A product with anti-aging and repairing effects, characterized in that: The anti-aging repair composition comprises the anti-aging repair composition according to any one of claims 1 to 4.

10. The product according to claim 9, characterized in that The product also includes a cosmetically, pharmaceutically, chemically or biologically acceptable carrier.

11. The product according to claim 9, characterized in that The anti-aging repair composition accounts for 1 to 99% of the total weight of the product.

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