A pesticide composition containing resveratrol

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing resveratrol. A pesticide composition containing resveratrol, the active ingredient of which is composed of osthole or allicin and resveratrol compounded. The present invention compounds osthole or allicin with resveratrol in a certain mass, showing a synergistic effect on various plant fungal disease pathogens, which can improve the control effect on plant diseases, providing data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and is of great significance for the high-quality and high-yield of crops such as wheat, peanut and maize.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing resveratrol. Background Art

[0002] Plant-derived pesticides refer to new pesticides directly or indirectly processed and synthesized from active substances with insecticidal or antibacterial effects extracted from plants, which cover active ingredients purified from plants, the plants themselves, and compounds synthesized after modifying the active structure. Plant-derived pesticides have attracted much attention and favor due to their environmental friendliness, diverse biological activities, low probability of pests developing resistance, and safety for non-target organisms. Currently, many plant-derived pesticide products have been registered for the prevention and control of pests in production, such as matrine, tea cicada net, veratrine, etc.

[0003] Resveratrol, also known as piceatannol, has the molecular formula C 14 H 12 O3. It is a non-flavonoid natural polyphenol compound naturally present in various plants such as grapes, peanuts, blueberries, and polygonum cuspidatum, and has various biological activities such as antioxidant, antibacterial and anti-inflammatory, anti-cardiovascular disease, and anti-tumor. Resveratrol has two isomers, cis and trans, and usually exists mainly in the trans configuration in nature. As a phytoalexin, resveratrol plays a protective role in plants, with functions such as resisting ultraviolet radiation and preventing fungal infections.

[0004] Existing studies have shown that resveratrol has been widely used in the fields of food, medicine, and daily chemicals due to its excellent functional activities such as antioxidant, antibacterial, anti-inflammatory, anti-tumor, and anti-cardiovascular in promoting human nutrition and health. Existing studies have shown that resveratrol can inhibit the proliferation of mammalian hepatocytes by inhibiting glycolysis, and hepatocytes are also relatively sensitive to resveratrol. At the same time, Parekh et al. found that resveratrol inhibits the growth of human HepG2 liver cancer cells by promoting apoptosis. Liu et al. found that the combination of resveratrol and cisplatin can reduce the absorption of glutamine by decreasing the expression of ASCT2 and enhance the anti-tumor activity of cisplatin. In addition, Dai et al. found that resveratrol inhibits the malignant progression of hepatocellular carcinoma through the regulation of the March1-induced PTEN / Akt signal transduction. The research results further indicate that resveratrol can inhibit the expansion of mammalian cancer cells by inducing apoptosis of cancer cells, inhibiting the growth of cancer cells, and enhancing the activity of chemotherapeutic drugs.

[0005] Currently, there is little research on resveratrol in the aspect of plant diseases. The research of our research group found that resveratrol has excellent antibacterial activity against Fusarium graminearum and Fusarium pseudograminearum, and has strong antibacterial activity against Sclerotium rolfsii, Rhizoctonia solani, Fusarium incarnatum, Fusarium equiseti, Fusarium proliferatum and Fusarium oxysporum, and has strong potential in preventing and controlling wheat scab, wheat basal rot, maize scab and peanut root rot.

[0006] In order to further expand the control spectrum and improve the control effect on plant diseases, the present invention further carried out the research on the binary compounding of resveratrol and candidate plant-derived fungicides magnolol, carvacrol, oregano oil, osthole or allicin. The results showed that when resveratrol was compounded with the candidate fungicides, it showed strong synergistic effects on Fusarium graminearum, Fusarium pseudograminearum, Sclerotium rolfsii, Rhizoctonia solani, Fusarium incarnatum, Fusarium equiseti, Fusarium proliferatum and Fusarium oxysporum, further indicating that resveratrol and its compound combinations have strong potential for preventing and controlling wheat scab, wheat basal rot, maize scab and peanut root rot. The present invention has important significance for effectively preventing and controlling wheat scab, wheat basal rot, maize scab and peanut root rot and further ensuring the safe production of wheat, maize and peanuts.

[0007] The information disclosed in this background art section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0008] The purpose of the present invention is to provide a pesticide composition containing resveratrol, which can effectively inhibit the growth of pathogenic bacteria such as wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, effectively prevent and control various plant fungal diseases, and has important significance for the high-quality and high-yield production of crops such as wheat, peanuts and maize.

[0009] To achieve the above purpose, the present invention provides the following technical solutions:

[0010] A pesticide composition containing resveratrol, the active ingredient of which is compounded by osthole or allicin and resveratrol.

[0011] Preferably, the mass ratio of osthole to resveratrol is 1-9:9-1.

[0012] Preferably, the mass ratio of allicin to resveratrol is 1-9:9-1.

[0013] The present invention also provides the use of the pesticide composition containing resveratrol in preventing and controlling fungal diseases of crops.

[0014] Preferably, the fungal diseases of crops include wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention combines osthole or allicin with resveratrol in a certain mass ratio, showing a synergistic effect on the pathogenic bacteria of various plant fungal diseases, which can improve the control effect on plant diseases, provide data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab, and is of great significance for the high-quality and high-yield of crops such as wheat, peanut, and maize. Specific Embodiments

[0017] The technical solution of the present invention will be clearly and completely described below in conjunction with...

[0018] Example

[0019] 1. Test Strains

[0020] Table 1 Test Strains

[0021]

[0022] 2. Test Agents

[0023] 99% osthole technical (Shanghai Aladdin Biochemical Technology Co., Ltd.), 98% allicin technical (Shanghai Yuanye Bio-Technology Co., Ltd.), 98% resveratrol technical (Shanghai Yuanye Bio-Technology Co., Ltd.).

[0024] After the test agents are dissolved, they are diluted into single-agent mother liquors with 0.1% Tween-80 aqueous solution. Multiple groups of ratios are set, and 5 mass concentration gradients are set for each single-agent mother liquor and the mixed agents of the ratios according to the equal ratio method.

[0025] 3. Test Methods

[0026] 9 mL of pre-melted PDA medium is added to a sterile conical flask. 1 mL of the liquid medicine is quantitatively sucked in sequence from low concentration to high concentration and added to the above conical flask respectively. After shaking well, it is poured into a petri dish with a diameter of 9 cm to make a drug-containing plate with the corresponding concentration. At the same time, a treatment without the agent is set as a blank control, and 10 plates are treated with the liquid medicine at each mass concentration. A mycelial cake is cut from the edge of the test strain colony with a punch with a diameter of 5 mm and inoculated in the center of the drug-containing plate and the blank control plate. After covering the petri dish lid, it is placed in an incubator at 25 °C for cultivation. When the colony diameter of the blank control grows to about 2 / 3 of the diameter of the petri dish, the colony diameter is measured by the cross method, and the inhibition rate of mycelial growth of different treatments is calculated.

[0027]

[0028] 4. Data analysis

[0029] The DPS software was used for data statistical analysis to obtain the virulence regression equation and the virulence EC 50 value of the agent against the target pathogen, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0030] 5. Determination results

[0031] The synergistic effect of the agent was evaluated based on the calculated co-toxicity coefficient (CTC). When CTC ≤ 80, it was an antagonistic effect; when 80 < CTC < 120, it was an additive effect; when CTC ≥ 120, it was a synergistic effect. The results are shown in Table 2-7.

[0032] Table 2 Indoor bioactivity determination of osthole and resveratrol complex against Sclerotium rolfsii

[0033] Name and ratio of medicaments EC50 (mg / L) ATI TTI CTC Osthole 24.8923 100.0000 -- -- Resveratrol 139.9665 17.7845 -- -- Osthole 9: Resveratrol 1 24.5208 101.5150 91.7784 110.6088 Osthole 8: Resveratrol 2 36.5088 68.1816 83.5569 81.5991 Osthole 7: Resveratrol 3 39.1165 63.6363 75.3353 84.4707 Osthole 6: Resveratrol 4 41.0724 60.6059 67.1138 90.3032 Osthole 5: Resveratrol 5 49.7847 49.9999 58.8922 84.9007 Osthole 4: Resveratrol 6 60.8479 40.9091 50.6707 80.7352 Osthole 3: Resveratrol 7 46.9398 53.0303 42.4491 124.9266 Osthole 2: Resveratrol 8 65.7158 37.8787 34.2276 110.6672 Osthole 1: Resveratrol 9 80.3031 30.9979 26.0060 119.1952

[0034] As can be seen from Table 2, when osthole and resveratrol were compounded at a mass ratio of 3:7, the co-toxicity coefficient of the compound combination against Sclerotium rolfsii was greater than 120, showing a synergistic effect.

[0035] Table 3 Indoor bioactivity determination of allicin and resveratrol complex against Sclerotium rolfsii

[0036]

[0037]

[0038] As can be seen from Table 3, when allicin and resveratrol were compounded, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations against Sclerotium rolfsii were greater than 120, showing a synergistic effect; especially when the mass ratio was 5:5, the co-toxicity coefficient of the compound combination against Sclerotium rolfsii reached 2001.2197, and the synergistic effect was the most significant.

[0039] Table 4 Indoor bioactivity determination of osthole and resveratrol complex against Fusarium graminearum

[0040] Name and ratio of medicaments EC50 (mg / L) ATI TTI CTC Osthole 15.1544 100.0000 -- -- Resveratrol 29.1159 52.0485 -- -- Osthole 9: Resveratrol 1 12.8512 117.9221 95.2049 123.8614 Osthole 8: Resveratrol 2 14.4173 105.1126 90.4097 116.2625 Osthole 7: Resveratrol 3 12.2241 123.9715 85.6146 144.8019 Osthole 6: Resveratrol 4 18.5829 81.5502 80.8194 100.9043 Osthole 5: Resveratrol 5 9.0853 166.8013 76.0243 219.4054 Osthole 4: Resveratrol 6 11.2162 135.1117 71.2291 189.6861 Osthole 3: Resveratrol 7 28.7302 52.7473 66.4340 79.3981 Osthole 2: Resveratrol 8 23.6259 64.1432 61.6388 104.0629 Osthole 1: Resveratrol 9 21.3738 70.9018 56.8437 124.7311

[0041] As can be seen from Table 4, when osthole and resveratrol were compounded, when the mass ratio was 3:7, the co-toxicity coefficient of the compound combination against Fusarium graminearum was less than 80, showing an antagonistic effect; when the mass ratio was 8:2, 6:4 or 2:8, the co-toxicity coefficient of the compound combination against Fusarium graminearum was between 80 and 120, showing an additive effect; the co-toxicity coefficients of the other compound combinations against Fusarium graminearum were all greater than 120, showing a synergistic effect.

[0042] Indoor Bioactivity Determination of the Compound of Allitridum and Resveratrol against Fusarium graminearum

[0043]

[0044]

[0045] As can be seen from Table 5, when allitridum and resveratrol are compounded, when the mass ratio is 9 - 1:1 - 9, the co-toxicity coefficient of the compound combination against Fusarium graminearum is greater than 120, showing a synergistic effect.

[0046] Indoor Bioactivity Determination of the Compound of Osthole and Resveratrol against Fusarium proliferatum

[0047] Name and ratio of medicaments EC50 (mg / L) ATI TTI CTC Osthole 25.4976 100.0000 -- -- Resveratrol 105.6220 24.1404 -- -- Osthole 9: Resveratrol 1 25.0580 101.7543 92.4140 110.1070 Osthole 8: Resveratrol 2 26.9142 94.7366 84.8281 111.6807 Osthole 7: Resveratrol 3 30.9227 82.4559 77.2421 106.7499 Osthole 6: Resveratrol 4 28.4974 89.4734 69.6562 128.4501 Osthole 5: Resveratrol 5 29.0673 87.7192 62.0702 141.3225 Osthole 4: Resveratrol 6 27.4974 92.7273 54.4843 170.1910 Osthole 3: Resveratrol 7 38.2465 66.6665 46.8983 142.1512 Osthole 2: Resveratrol 8 35.4479 71.9298 39.3123 182.9700 Osthole 1: Resveratrol 9 33.7992 75.4385 31.7264 237.7784

[0048] As can be seen from Table 6, when osthole and resveratrol are compounded, when the mass ratio is in the range of 6 - 1:4 - 9, the co-toxicity coefficient of the compound combination against Fusarium proliferatum is greater than 120, showing a synergistic effect; the co-toxicity coefficients of the other compound combinations against Fusarium proliferatum are between 80 and 120, showing an additive effect.

[0049] Indoor Bioactivity Determination of the Compound of Allitridum and Resveratrol against Fusarium proliferatum

[0050]

[0051]

[0052] As can be seen from Table 7, when allitridum and resveratrol are compounded, when the mass ratio is 8:2 or 7:3 or 6:4, the co-toxicity coefficient of the compound combination against Fusarium proliferatum is between 80 and 120, showing an additive effect; the co-toxicity coefficients of the other compound combinations against Fusarium proliferatum are greater than 120, showing a synergistic effect.

[0053] In summary, in the present invention, osthole or allitridum and resveratrol are compounded at a certain mass ratio, showing a synergistic effect against the pathogens of various plant fungal diseases, which can improve the control effect on plant diseases, providing data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and having important significance for the high quality and high yield of crops such as wheat, peanut and maize.

[0054] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many modifications and variations are possible in light of the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention, as well as various different selections and modifications. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. Use of a pesticide composition containing resveratrol in controlling crop diseases caused by Sclerotium rolfsii, Fusarium graminearum, and Fusarium proliferatum, characterized in that, The active ingredient of the pesticide composition containing resveratrol is composed of osthole or allicin and resveratrol in combination; When controlling crop diseases caused by Sclerotium rolfsii, the mass ratio of osthole to resveratrol is 3:7; the mass ratio of allicin to resveratrol is 1-9:9-1; When controlling crop diseases caused by Fusarium graminearum, the mass ratio of osthole to resveratrol is 9:1, 7:3, 5:5, 4:6 or 1:9; the mass ratio of allicin to resveratrol is 1-9:9-1; When controlling crop diseases caused by Fusarium proliferatum, the mass ratio of osthole to resveratrol is 6-1:4-9; the mass ratio of allicin to resveratrol is 9:1 or 5-1:5-9.

Images