A method for controlling pests in an open culture system of microalgae based on light-induced coupling

Abstract

The present application belongs to the field of bioengineering, and particularly relates to a method for controlling pests in an open culture system of microalgae based on light-induced coupling electric inhibition. The main steps are as follows: a light irradiation device and a high-voltage discharge device are arranged on one side of a raceway pond; first, light-induced treatment is performed, and the raceway pond is irradiated at the same time, the irradiated light at the left curve and the right curve is blue light with an intensity of 1500-2000 Lux; the irradiated light at the upper raceway and the lower raceway is red light with an intensity of 1000-2000 Lux; so as to attract rotifers to the left curve and the right curve to form rotifer aggregation areas; then, the rotifer aggregation areas at the left curve and the right curve are subjected to electric shock treatment; the present application can kill rotifers while protecting the survival of algae as much as possible, and can effectively remove most of the pest pollution through the combined regulation of light induction and high-voltage discharge, so as to ensure that the growth of microalgae biomass and the accumulation of bioactive substances are less affected, and the application prospect is wide.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of bioengineering, and particularly relates to a method for controlling pests in an open culture system of microalgae based on light-induced coupling electric inhibition. BACKGROUND

[0002] Microalgae are rich in protein, fat, carbohydrates, vitamins, chlorophyll and other nutrients, and have high nutritional value, and are widely used in food, medicine, cosmetics, fuel, environmental purification and other fields. Therefore, factories will use open raceway ponds for outdoor cultivation to obtain their own economic benefits. However, the outdoor raceway pond cultivation is easily affected by external factors, especially the invasion of rotifers. Rotifers can cause the microalgae cultivation system to collapse rapidly. Therefore, how to selectively remove rotifers mixed in microalgae is crucial.

[0003] The existing common rotifer removal techniques include filtration method, addition of chemical reagents, suffocation method, and method of changing culture conditions. Since the microalgae cells are smaller than the rotifers, they can be separated by filter screens, but the filtration method can only be used for small-scale cultivation. The number of microalgae in outdoor large-scale cultivation is huge, and it is easy to break the filter screen, and this method is time-consuming and labor-intensive. In addition, some researchers have proposed adding chemical reagents to kill pests, such as bleach, pesticides, and allelochemicals. These chemical reagents can kill pests, but since the rotifers and microalgae are mixed together, the addition of chemical reagents will poison the microalgae. Therefore, how to control the amount of chemical reagents to balance the pest killing and algae cultivation remains to be explored. In addition, if the chemical reagents are not handled properly, they will pollute the environment. The suffocation method is a new method proposed for environmental protection, which introduces CO2 and N2 into water to reduce the dissolved oxygen in water, thereby killing rotifers. However, this method has limitations. It is only effective for ciliates, but not for paramecia. Moreover, CO2 and N2 are expensive, and their use in outdoor cultivation is costly. Changing the culture conditions involves changing the pH, salinity, and temperature of the culture system to inhibit the reproduction of rotifers. However, in practice, the culture environment of some microalgae and rotifers is highly compatible, and it is difficult to achieve the effect of removing pests by changing the culture conditions.

[0004] Most of the existing technologies have the disadvantages of high cost, environmental pollution, small application range, and poor pest killing effect. Therefore, how to save costs and protect the environment while achieving high-efficiency pest killing is a problem that needs to be solved. SUMMARY

[0005] In order to solve one of the problems existing in the prior art, the present application provides a method for controlling pests such as rotifers based on light-induced coupling and high-voltage discharge.

[0006] In order to achieve the above purpose, the present application adopts the following solutions:

[0007] A method for controlling pests in an open culture system of microalgae based on light-induced coupling electric suppression, specifically comprising the following steps:

[0008] (1) Microalgae culture: first, prepare a microalgae culture medium and pour it into a microalgae breeding raceway pond, with a height of 2 / 5-3 / 5 of the height of the raceway pond, then inoculate microalgae in the logarithmic growth phase into the culture medium in the raceway pond, set the light conditions to 4000-5000 Lux, and the temperature to 16-30℃, and culture the microalgae, the culture period of the microalgae being 7-15 days;

[0009] (2) Light-induced treatment: a light device and a high-voltage discharge device are provided on one side of the raceway pond; before starting the light device, the microalgae breeding raceway pond is kept in a dark environment, and the propeller in the raceway pond is stopped; and the biomass of the microalgae must be maintained at 0.4 g / L or more or the microalgae are in the logarithmic growth phase;

[0010] The raceway of the raceway pond is divided into four areas, which are sequentially referred to as the upper raceway, the lower raceway, the left curve, and the right curve; then the light device is started, and the upper raceway, the lower raceway, the left curve, and the right curve of the raceway pond are illuminated, the light in the left curve and the right curve being blue light with an intensity of 1500-2000 Lux; the light in the upper raceway and the lower raceway being red light with an intensity of 1000-2000 Lux; so as to attract rotifers to the left curve and the right curve to form a rotifer aggregation area;

[0011] (3) After the light-induced treatment of step (2), the high-voltage discharge device is used to electrically shock the rotifer aggregation area in the left curve and the right curve, and after the electric shock treatment, the rotifer aggregation area is killed.

[0012] After the above steps, the rotifers in the rotifer aggregation area are directly killed by high-voltage pulse electricity, thereby greatly reducing the number of rotifers in the microalgae, and since the discharge current is small when the high-voltage pulse power source is contacted, the microalgae are not damaged.

[0013] Preferably, the microalgae culture medium in step (1) is composed of artificial seawater or seawater and f / 2 culture nutrient salt components; the f / 2 culture nutrient salt components are: NaNO3 75 mg / L, NaH2PO4·H2O 5 mg / L, Na2SiO3·9H2O 30 mg / L, FeCl3·6H2O 3.15 mg / L, Na2EDTA·2H2O 4.36 mg / L, MnCl2·4H2O 0.18 mg / L, ZnSO4·7H2O 0.022 mg / L, CoCl2·6H2O 0.01 mg / L, CuSO4·5H2O 0.0098 mg / L, NaMoO4·2H2O 0.0063 mg / L.

[0014] Preferably, the inoculation amount of the microalgae in step (1) is 0.2 g / L; the microalgae include Nannochloropsis;

[0015] Preferably, the light illumination device in step (2) is composed of a light-emitting diode, a direct current power supply box and a time relay, and the illumination time is controlled by the time relay.

[0016] Preferably, the high-voltage discharge device in step (2) is a high-voltage pulse power supply box, which comprises a voltage control panel and a frequency control panel; the electric field intensity is controlled by the voltage control panel, and the electric shock frequency is controlled by the frequency control panel.

[0017] Preferably, when the upper runway is illuminated in step (2), the illumination area is located at the middle position of the upper runway, and the illumination area of the lower runway is symmetrical to the illumination area of the upper runway; similarly, the illumination area of the left curve is symmetrical to the illumination area of the right curve; wherein the light intensity of the left curve and the right curve is 1800-2000 Lux; the light intensity of the upper runway and the lower runway is 1500-1600 Lux, and the illumination time is 10-60 min.

[0018] Preferably, the voltage density of the electric shock in step (3) is 1200-1500 V / cm, the frequency of the electric shock is 0.4-1 s, the duty cycle is 5-10%, and the discharge time is 2-60 min.

[0019] Compared with the traditional insecticidal method, the present application has at least the following beneficial effects:

[0020] The present application utilizes the phototactic behavior of rotifers, concentrates most of the rotifers in a specific area of the runway pool by using a light source, and kills the rotifers by electric shock, thereby effectively reducing the number of rotifers in the runway pool and reducing the damage to algae caused by electric shock. Compared with the traditional filtration method and the method of adding chemical reagents, the present method has high efficiency, small environmental pollution, conforms to the current environmental protection concept, and can effectively solve the problem of rotifer pest in industrial cultivation.

[0021] In addition, the application is simple in operation, wide in application range, and high in time utilization rate by being staggered with the operation time of the raceway pond, and has a broad application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 Schematic diagram of processing of light-induced coupling high-voltage discharge in a raceway pond

[0023] Figure 2 Taxis rate of rotifer under irradiation of different light colors in Example 1.

[0024] Figure 3 Taxis rate of rotifer under irradiation of different intensity blue light and red light in Example 1.

[0025] Figure 4 Biomass concentration of Nannochloropsis in the mixed solution of Nannochloropsis and rotifer under light-induced electric shock, electric shock only and blank treatment in Examples 2 and 3.

[0026] Figure 5 Number of rotifer in the mixed solution of Nannochloropsis and rotifer under light-induced electric shock, electric shock only and blank treatment in Examples 2 and 3. DETAILED DESCRIPTION

[0027] In order to more clearly illustrate the overall concept of the present application, the technical solutions of the present application will be clearly and completely described below in combination with specific embodiments.

[0028] The microalgae selected in the examples is Nannochloropsis;

[0029] Example 1:

[0030] (1) The culture medium of Nannochloropsis is composed of artificial seawater and f / 2 culture nutrient salt; wherein the f / 2 culture nutrient salt is composed of NaNO375 mg / L, NaH2PO4·H2O 5 mg / L, Na2SiO3·9H2O 30 mg / L, FeCl3·6H2O 3.15 mg / L, Na2EDTA·2H2O 4.36 mg / L, MnCl2·4H2O 0.18 mg / L, ZnSO4·7H2O 0.022 mg / L, CoCl2·6H2O 0.01 mg / L, CuSO4·5H2O 0.0098 mg / L, and NaMoO4·2H2O 0.0063 mg / L.

[0031] The runway pool is a transparent acrylic plate container with a size of 600*200*250. The runway of the runway pool is divided into four areas, which are sequentially recorded as the upper runway, the lower runway, the left side curve and the right side curve. The runway pool is sterilized and cleaned in advance using sodium hypochlorite solution, and the cleaned runway pool is placed under sunlight (5-15 min) to remove residual chlorine in the runway pool, and finally a pretreated runway pool is obtained. Then the culture medium is poured into the microalgae breeding runway pool to a height of 2 / 5 of the height of the runway pool, and the microalgae in the logarithmic growth phase is inoculated at an inoculation amount of 0.2 g / L. The light intensity is controlled at 5000 Lux, the temperature is controlled at 25°C, and the culture period is 7 days.

[0032] (2) Runway pool pest simulation experiment:

[0033] When the Nannochloropsis biomass rises to 0.4 g / L, pour the Brachionus plicatilis into the runway pool so that the number of rotifers in the runway pool is controlled at 20 tails / mL, and rotate the runway pool by the propeller for fifteen minutes to fully mix the rotifers and Nannochloropsis.

[0034] (3) Apply light source: A light device and a high-voltage discharge device are provided on one side of the runway pool. The light device is composed of a light-emitting diode, a direct current power supply box and a time relay, and the light time is controlled by the time relay. The high-voltage discharge device is a high-voltage pulse power supply box (Suzhou Juyongda Electronics Technology Co., Ltd.), which includes a voltage control panel and a frequency control panel. The electric field strength is controlled by the voltage control panel, and the electric shock frequency is controlled by the frequency control panel.

[0035] Before starting the light device, the microalgae breeding runway pool is in a dark environment, and the propeller in the runway pool is stopped rotating. Then start the light device, first use red, orange, yellow, green, blue, white and purple light to apply light treatment to the right side curve area of the runway pool, and control the light intensity at 2000 Lux and the light time at 20 min. Observe the number of rotifers before and after light and calculate the phototaxis rate of rotifers, as shown in Figure 2 The results show that blue light has positive phototaxis compared with other light, and is the most significant; red light has negative phototaxis compared with other light, and is the most significant.

[0036] (4) Select red and blue light from the above light sources, respectively, to explore the effect of 1000 Lux, 1500 Lux, 2000 Lux, 2500 Lux and 3000 Lux blue light and red light on the phototaxis of rotifers, and the light control time is 20 min. Observe the number of rotifers before and after light and calculate the phototaxis rate of rotifers; the phototaxis rate formula is as follows:

[0037] A=(N-M) / M

[0038] Wherein A is the phototaxis rate of rotifers, M is the number of rotifers before light (tails / mL), and N is the number of rotifers after light (tails / mL)

[0039] Results are shown in Figure 3 Table 1. The results show that blue light and red light have the strongest positive phototaxis and negative phototaxis at 2000 Lux and 1500 Lux, respectively. If the light intensity is too low, the effect is not obvious. If the light intensity is too high, it will cause damage to the vision of rotifer, so it will appear photophobic behavior. If the light intensity is further increased, it will disturb the phototaxis behavior of rotifer, resulting in the loss of phototaxis behavior of rotifer.

[0040] Example 2

[0041] (1) The culture medium of Nannochloropsis is composed of artificial seawater and f / 2 culture nutrient salt. The composition of f / 2 culture nutrient salt is: NaNO375mg / L, NaH2PO4·H2O 5mg / L, Na2SiO3·9H2O 30mg / L, FeCl3·6H2O 3.15mg / L, Na2EDTA·2H2O 4.36mg / L, MnCl2·4H2O 0.18mg / L, ZnSO4·7H2O 0.022mg / L, CoCl2·6H2O 0.01mg / L, CuSO4·5H2O 0.0098mg / L, NaMoO4·2H2O 0.0063mg / L.

[0042] The runway pool is a transparent acrylic plate container with a size of 600*200*250. The runway of the runway pool is divided into four areas, which are sequentially recorded as upper runway, lower runway, left curve and right curve. The runway pool is sterilized and cleaned with sodium hypochlorite solution in advance, and the cleaned runway pool is placed under sunlight (5-15min) to remove residual chlorine in the runway pool, and finally a pretreated runway pool is obtained. Then the culture medium is poured into the microalgae breeding runway pool with a height of 2 / 5 of the height of the runway pool, and the microalgae in logarithmic growth phase is inoculated at an inoculation amount of 0.2g / L. The light intensity is controlled at 5000Lux, and the temperature is controlled at 25℃. The culture period is 7d.

[0043] (2) Runway pool pest simulation experiment:

[0044] When the biomass of Nannochloropsis reaches 0.4g / L, pour the Brachionus plicatilis into the runway pool to control the number of rotifers in the runway pool at 20 tails / mL, and rotate the runway pool for fifteen minutes to fully mix the rotifers and Nannochloropsis.

[0045] (3) A light device and a high-voltage discharge device are provided on one side of the runway pool. The light device is composed of a light-emitting diode, a direct current power supply box and a time relay. The light time is controlled by the time relay. The high-voltage discharge device is a high-voltage pulse power supply box (Suzhou Juyongda Electronics Technology Co., Ltd.), which includes a voltage control panel and a frequency control panel. The electric field strength is controlled by the voltage control panel, and the electric shock frequency is controlled by the frequency control panel.

[0046] Without turning on the light device, only the high-voltage discharge device is used to perform electric shock treatment on the left and right curves, the control voltage density is 2000V / cm, the electric shock time is 1h, the electric shock frequency is 1s, and the duty cycle control is 5%.

[0047] Example 3

[0048] (1) The culture medium of Nannochloropsis oceanica is composed of artificial seawater and f / 2 culture nutrient salt; wherein the f / 2 culture nutrient salt is composed of NaNO375mg / L, NaH2PO4·H2O 5mg / L, Na2SiO3·9H2O 30mg / L, FeCl3·6H2O 3.15mg / L, Na2EDTA·2H2O 4.36mg / L, MnCl2·4H2O 0.18mg / L, ZnSO4·7H2O 0.022mg / L, CoCl2·6H2O 0.01mg / L, CuSO4·5H2O 0.0098mg / L, NaMoO4·2H2O 0.0063mg / L.

[0049] The runway pool is a transparent acrylic plate container with a size of 600*200*250. The runway of the runway pool is divided into four areas, which are sequentially recorded as the upper runway, the lower runway, the left curve and the right curve. The runway pool is sterilized and cleaned with sodium hypochlorite solution in advance, and then placed in the sunlight to remove the residual chlorine in the runway pool. Finally, a pretreated runway pool is obtained. Then the culture medium is poured into the microalgae breeding runway pool to a height of 2 / 5 of the height of the runway pool, and the logarithmic growth period of microalgae is inoculated at an inoculation amount of 0.2g / L. The light intensity is controlled at 5000Lux, the temperature is 25℃, and the culture period is 7d.

[0050] (2) Runway pool pest simulation experiment:

[0051] When the biomass of Nannochloropsis oceanica reaches 0.4g / L, pour the Brachionus plicatilis into the runway pool to control the number of rotifers in the runway pool at 20 tails / mL, and rotate the runway pool for fifteen minutes to fully mix the rotifers and Nannochloropsis oceanica.

[0052] (3) Apply light source: A light device and a high-voltage discharge device are provided on one side of the runway pool. The light device is composed of a light-emitting diode, a direct current power supply box and a time relay. The light time is controlled by the time relay. The high-voltage discharge device is a high-voltage pulse power supply box (Suzhou Juyongda Electronics Technology Co., Ltd.), which includes a voltage control panel and a frequency control panel. The electric field strength is controlled by the voltage control panel, and the electric shock frequency is controlled by the frequency control panel.

[0053] First, light induction; before starting the light device, the microalgae culture runway pool is in a dark environment, and the propeller in the runway pool is stopped; then the light device is started and the upper runway, the lower runway, the left curve and the right curve of the runway pool are illuminated; the illumination area is located in the middle position of the upper runway, and the illumination area of the lower runway is symmetrical to the illumination area of the upper runway; similarly, the illumination area of the left curve is symmetrical to the illumination area of the right curve; the illumination light in the left curve and the right curve is blue light (rotifer phototaxis), and the light intensity is controlled at 2000Lux; the illumination light in the upper runway and the lower runway is red light (rotifer photophobia), and the light intensity is controlled at 1500Lux; the illumination time is controlled at 20min; so as to attract the rotifers to the left curve and the right curve to form a rotifer gathering area;

[0054] (4) High-voltage discharge treatment: after the light induction treatment of step (3), the rotifer gathering area illuminated by blue light in the left curve and the right curve is subjected to electric shock treatment by using a high-voltage discharge device, the control voltage density is 2000V / cm, the electric shock time is 1h, the electric shock frequency is 1s, and the duty cycle control is 5%.

[0055] Blank group:

[0056] (1) The culture medium of Nannochloropsis is composed of artificial seawater and f / 2 culture nutrient salt; wherein the f / 2 culture nutrient salt is composed of NaNO375mg / L, NaH2PO4·H2O 5mg / L, Na2SiO3·9H2O 30mg / L, FeCl3·6H2O 3.15mg / L, Na2EDTA·2H2O 4.36mg / L, MnCl2·4H2O 0.18mg / L, ZnSO4·7H2O 0.022mg / L, CoCl2·6H2O 0.01mg / L, CuSO4·5H2O 0.0098mg / L, NaMoO4·2H2O 0.0063mg / L.

[0057] The runway pool is a transparent acrylic plate container with a size of 600*200*250; the runway pool is sterilized and cleaned with sodium hypochlorite solution in advance, and the cleaned runway pool is placed under sunlight to remove residual chlorine in the runway pool, and finally a pretreated runway pool is obtained; then the culture medium is poured into the microalgae culture runway pool, the height of which is 2 / 5 of the height of the runway pool, and the logarithmic growth period microalgae is inoculated at an inoculation amount of 0.2g / L, the light intensity is controlled at 5000Lux, the temperature is controlled at 25℃, and the culture period is 7d;

[0058] (2) Runway pool pest simulation experiment:

[0059] When the microalgae biomass reaches 0.4 g / L, pour the Brachionus plicatilis into the raceway pond so that the number of the rotifer in the raceway pond is controlled at 20 per mL, and rotate the raceway pond for 15 minutes so that the rotifer and the microalgae are mixed thoroughly.

[0060] Result determination:

[0061] Observe the microalgae biomass and the number of the rotifer in the raceway pond every day;

[0062] Result explanation:

[0063] According to Figure 4 It is shown that after the light-induced electric shock (Example 3), the biomass in the raceway pond generally shows an upward trend, while the blank group and the electric shock only group (Example 2) generally show a downward trend, and the biomass in the blank group approaches zero after the sixth day, at which time the biomass of the light-induced electric shock group (Example 3) is 52.5 times that of the blank group, and 3.89 times that of the electric shock only group (Example 2), at which time the biomass can reach 1.31 g / L and still shows an upward trend.

[0064] At the same time, according to Figure 5 It is shown that after the light-induced electric shock (Example 3), the number of the rotifer in the raceway pond generally shows a downward trend, while the blank group and the electric shock only group (Example 2) both show an upward trend; and the number of the rotifer is measured after 7 days of culture, the number of the rotifer in the experimental group is 1 / 10 of that of the blank group, and 1 / 7 of that of the electric shock only group, which is very significant.

[0065] In summary, the effective combination of the light induction and the high-voltage discharge can efficiently remove most of the rotifer in the raceway pond, and reduce the damage of the electric shock to the microalgae, and achieve an unexpected significant effect.

[0066] Description: The above examples are only used to illustrate the technical solutions described in the present application and do not limit the present application; therefore, although the present application has been described in detail with reference to the above examples, those skilled in the art should understand that the present application can still be modified or replaced equivalently; and all technical solutions and improvements that do not deviate from the spirit and scope of the present application should be covered in the scope of the claims of the present application.

Claims

1. A method for controlling pests in an open culture system of microalgae based on photo-induced electric suppression, characterized in that, The method comprises the following steps: (1) Microalgae culture: first, prepare a microalgae culture medium and pour it into a microalgae cultivation raceway pond, with a height of 2 / 5-3 / 5 of the height of the raceway pond, then inoculate microalgae in the logarithmic growth phase into the culture medium in the raceway pond, set the light conditions to 4000-5000 Lux, and the temperature to 16-30℃, and culture the microalgae, with a culture period of 7-15 days; (2) Light induction treatment: a light device and a high-voltage discharge device are arranged on one side of the raceway pond; before starting the light device, the microalgae cultivation raceway pond is kept in a dark environment, and the rotating paddle in the raceway pond is controlled to stop rotating; and the microalgae biomass must be maintained at more than 0.4 g / L or the microalgae are in the logarithmic growth phase; The raceway of the raceway pond is divided into four areas, which are sequentially referred to as the upper raceway, the lower raceway, the left curve, and the right curve; then the light device is started, and the upper raceway, the lower raceway, the left curve, and the right curve of the raceway pond are irradiated, the irradiation light in the left curve and the right curve is blue light with an intensity of 1500-2000 Lux, and the irradiation light in the upper raceway and the lower raceway is red light with an intensity of 1000-2000 Lux; so as to attract rotifers to the left curve and the right curve to form a rotifer gathering area; (3) After the light induction treatment of step (2), the high-voltage discharge device is used to perform electric shock treatment on the rotifer gathering area in the left curve and the right curve, the voltage density of the electric shock is 1200-1500 V / cm, the frequency of the electric shock is 0.4-1 s, the duty cycle is 5-10%, and the discharge time is 2-60 min; after the electric shock treatment, the concentrated area of the rotifers can be disinfected.

2. The method for controlling pests in open culture system of microalgae based on photo-induced electric suppression according to claim 1, characterized in that, The microalgae culture medium in step (1) is composed of artificial seawater or seawater and f / 2 culture nutrient salt components; the f / 2 culture nutrient salt components are: NaNO375 mg / L, NaH2PO4·H2O 5 mg / L, Na2SiO3·9H2O 30 mg / L, FeCl3·6H2O 3.15 mg / L, Na2EDTA·2H2O 4.36 mg / L, MnCl2·4H2O 0.18 mg / L, ZnSO4·7H2O 0.022 mg / L, CoCl2·6H2O 0.01 mg / L, CuSO4·5H2O 0.0098 mg / L, and NaMoO4·2H2O 0.0063 mg / L.

3. The method for controlling pests in open culture system of microalgae based on photo-induced electric suppression according to claim 1, characterized in that, The microalgae in step (1) include Nannochloropsis.

4. The method for controlling pests in open culture system of microalgae based on photo-induced electric suppression according to claim 1, characterized in that, The light device in step (2) is composed of a light-emitting diode, a direct-current power supply box, and a time relay, and the light time is controlled by the time relay.

5. The method for controlling pests in open culture system of microalgae based on photo-induced electric suppression according to claim 1, characterized in that, The high-voltage discharge device in step (2) is a high-voltage pulse power supply box, which includes a voltage control panel and a frequency control panel; the electric field intensity is controlled by the voltage control panel, and the electric shock frequency is controlled by the frequency control panel.

6. The method for controlling pests in open culture system of microalgae based on photo-induced electric suppression according to claim 1, characterized in that, In the step (2), the light irradiation area is located in the middle of the upper track, and the light irradiation area of the lower track is symmetrical to the light irradiation area of the upper track; similarly, the light irradiation area of the left curve is symmetrical to the light irradiation area of the right curve.

7. The method for controlling pests in open culture system of microalgae based on photo-induced electric suppression according to claim 6, characterized in that, The light intensity of the left curve and the right curve is 1800-2000 Lux; the light intensity of the upper track and the lower track is 1500-1600 Lux, and the light irradiation time is 10-60 min.

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