Laminarin-sophora flavescens gum-silk microfiber medical catheter and preparation method thereof

Abstract

The present application provides a kind of laminarin-western radix astragali glue-silk microfiber medical catheter and its preparation method, and the medical catheter preparation solution contains the following components per 100ml: laminarin 1-10g, aqueous solution 10-100mL, 10% radix astragali glue solution 2-30mL, silk microfiber 1-10g.The material of the present application is low in price, and the preparation method is simple and easy to operate, which avoids the cytotoxicity side effects caused by the use of toxic crosslinking agent, has good biocompatibility, and at the same time, the prepared biomimetic gel catheter has good porosity, mechanical strength and good flexibility, is suitable for blood vessels, peripheral nerve catheter and other applications, and is easy to metabolize in vivo.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medical materials, and particularly relates to a porous 3D laminarin-silk fibroin microporous medical biomimetic gel catheter and a preparation method thereof. BACKGROUND

[0002] Medical catheters have long been a necessity for medical behavior, and usually need to be implanted in the human body for traumatic diagnosis and treatment. For example, indwelling catheters in blood vessels, central venous catheters, urinary catheters used in urinary tract treatment, and tracheal intubation are all medical behaviors that are necessary and frequently performed in general medical operations. Peripheral nerve defects caused by trauma are relatively common in clinical practice. However, when long-distance nerve defects cannot be compensated for nerve loss by end-to-end suturing, catheters are needed to bridge the repair. In addition to the above-mentioned traditional medical catheters, with the rapid development of medical technology, multi-lumen and composite catheter types have also emerged, and the time left in the body has also increased. They have become important tools for blood flow monitoring, infusion, hemodialysis, drug delivery, and intravenous nutrition support in medical behavior, showing a dramatic increase in the use and demand for medical catheters.

[0003] Laminarin is a macromolecular polysaccharide substance in laminaria. Research reports that laminarin has very wide biological activity. Laminarin can reduce urinary protein, purify blood uric acid, and improve blood lipid concentration, and has a good preventive and therapeutic effect on gout, hyperlipidemia, and early and middle stage renal failure. Another study reports that laminarin has a wide application in preparing drugs that can inhibit rejection reactions of transplanted organs and induce immune tolerance. Laminarin can inhibit rejection reactions after organ transplantation and significantly prolong the survival time of transplants.

[0004] Western Astragalus gum, also known as western Astragalus gum and Astragalus gum, is one of the most important natural biological macromolecular materials. It is inexpensive and easy to obtain, has good biocompatibility and degradability, and has been widely used in food, medicine and chemical industries. It has film-forming property and gelling effect, and can be used as a retarder for controlled-release preparations to regulate drug release.

[0005] Moreover, silk microfibers are non-toxic to the human body, safe and reliable, have good biocompatibility, are suitable for the development of functional materials, are inexpensive and easy to obtain, have good biocompatibility and degradability, and have been widely used in medicine, health, food, chemical industry and many other fields. It is one of the most important natural biological macromolecular materials.

[0006] Although laminarin, western Astragalus gum and silk microfibers have the above-mentioned advantages, there are few reports on the use of mixtures or polymers of laminarin, western Astragalus gum and silk microfibers to prepare medical catheters. SUMMARY

[0007] The technical problem solved by the present application is to provide a porous 3D laminarin-sciadopitysin-silk microfiber medical conduit and a preparation method thereof, which is low in material price, simple in preparation method, easy to operate, avoids cytotoxic side effects caused by the use of toxic cross-linking agents, has good biocompatibility, and has good porosity, mechanical strength and good flexibility.

[0008] To solve the above technical problems, an embodiment of the present application provides a laminarin-sciadopitysin-silk microfiber medical conduit, which is a hollow circular tube mesh support, and the support is loaded with a mixed solution of laminarin-sciadopitysin-silk microfiber.

[0009] The 10% sciadopitysin solution is prepared from sciadopitysin and pure water, and the mass percentage of sciadopitysin and pure water is 1:9.

[0010] The 10% sciadopitysin solution is prepared from sciadopitysin and pure water, and the mass percentage of sciadopitysin and pure water is 1:9.

[0011] Preferably, the medical conduit preparation solution contains the following components per 100 ml: 5 g of laminarin, 60 ml of water solution, 20 ml of 10% sciadopitysin solution, and 6 g of silk microfiber.

[0012] An embodiment of the present application also provides a preparation method of the laminarin-sciadopitysin-silk microfiber medical conduit, which mainly includes gel preparation, mold shaping, sample demolding, and sterilization treatment stages, and comprises the following steps:

[0013] S1, preparing a silk microfiber solution: a formula amount of silk microfiber is dissolved in 10-100 mL of water solution, and heated and stirred to completely dissolve;

[0014] S2, weighing a formula amount of 10% sciadopitysin solution, slowly pouring the 10% sciadopitysin solution into the silk microfiber solution prepared in step S1, and fully stirring and dissolving with a glass rod;

[0015] S3, weighing a formula amount of laminarin, adding the mixed solution obtained in step S2, fully stirring and dissolving, and applying intermittent pulse ultrasonic waves with an acoustic pressure of 0.05 MPa for 10 min / time, a total of 3 times, with an interval of 5 min each time;

[0016] S4, using a 10ml syringe to draw the medical catheter preparation solution of step S3, inject into the catheter mold, fixed with medical catheter preparation solution containing catheter mold, flat in the glass tray, placed in the refrigerator overnight;

[0017] S5, take out the catheter mold in step S4 refrigerator, remove the outer layer of the catheter mold, form the catheter mold wrapped core, clamp the catheter mold wrapped core into the freeze-dried bottle with tweezers, cover the bottle plug, horizontal into the refrigerator, freeze;

[0018] S6, take out the freeze-dried bottle after freezing in step S5, use the freeze-drying machine to completely freeze the freeze-dried bottle, and take out the catheter mold wrapped core, carefully remove the core in the catheter mold, get laminarin-silk fibroin-silk fibroin medical catheter;

[0019] S7, the prepared laminarin-silk fibroin-silk fibroin medical catheter is put into a sealed bag, and the name and preparation time are marked. It can be stored for a long time in a cool, dry place. Before use, take out the catheter and sterilize it with Co60 irradiation. It is ready for use.

[0020] Preferably, the preparation method of the above laminarin-silk fibroin-silk fibroin medical catheter is as follows:

[0021] S1, prepare silk fibroin solution: take 6g silk fibroin and dissolve in 60ml water solution, heat and stir until completely dissolved;

[0022] S2, weigh 20ml 10% silk fibroin solution, slowly pour into the silk fibroin solution prepared in step S1, and stir with a glass rod until dissolved;

[0023] S3, weigh 5g laminarin, add to the mixed solution prepared in step S2, ultrasonic stirring at 70℃ for 2.5h, make it fully dissolved, and use intermittent sound pressure 0.05MPa pulse ultrasonic action for 10min / time, 3 times, with 5min interval each time, which can increase the porosity of the catheter;

[0024] S4, using a 10ml syringe to draw the medical catheter preparation solution of step S3, inject into the catheter mold, fixed with medical catheter preparation solution containing catheter mold, flat in the glass tray, placed in the refrigerator overnight;

[0025] S5, take out the catheter mold in step S4 refrigerator, remove the outer layer of the catheter mold, form the catheter mold wrapped core, clamp the catheter mold wrapped core into the freeze-dried bottle with tweezers, cover the bottle plug, horizontal into the refrigerator, -20℃ freeze for 2h;

[0026] S6, open the freeze dryer, when the temperature is reduced to below -40 DEG C, the vacuum is reduced to 0.02 Pa, remove the frozen freeze-dried bottle in step S5, remove the freeze-dried bottle and remove the inner core of the pipe mold in the package, carefully remove the inner core in the pipe mold, get laminarin-silk floss medical conduit of western Chinese milk vetch glue;

[0027] S7, the prepared laminarin-silk floss medical conduit of western Chinese milk vetch glue is put into a sealed bag, and the name and preparation time are marked. It can be stored for a long time in a cool and dry place. Before use, the conduit is irradiated by Co60 and sterilized for standby.

[0028] Wherein, the conduit mold comprises an outer tube, an inner core, a lower stopper and an upper pressure head, the lower stopper is a reverse T-shaped structure, a lower stopper center hole with an open upper end and a closed lower end is arranged in the lower stopper, the lower end of the inner core is inserted into the lower stopper center hole, the upper end of the inner core is inserted into the lower end of the outer tube and extends above the upper end of the outer tube, the upper part of the lower stopper is inserted into the lumen of the outer tube, and the lower part is limited below the outer tube;

[0029] The upper pressure head is a T-shaped structure, an upper pressure head center hole with open upper and lower ends is arranged in the upper pressure head, the upper pressure head is sleeved between the outer tube and the inner core, and the lower part is inserted into the lumen of the outer tube and the upper part is limited above the outer tube, the gap between the upper pressure head and the lower stopper is a medical conduit preparation solution filling area, and the outer tube body is also provided with an opening hole for injecting the medical conduit preparation solution, and the medical conduit preparation solution is injected into the conduit preparation solution filling area along the opening hole.

[0030] The beneficial effects of the above technical solutions of the present application are as follows:

[0031] The present application adopts physical mixing and low-intensity pulse ultrasonic process, and the method for preparing the porous 3D laminarin-silk floss medical biomimetic gel conduit of western Chinese milk vetch glue by the ultrasonic cavitation effect makes the porosity greater than 65%, the prepared biomimetic gel conduit has good porosity, mechanical strength and good flexibility, is suitable for blood vessels, peripheral nerve conduits and other applications, is easy to metabolize in vivo, the material is low in price, the preparation method is simple and easy to operate, the cytotoxic side effects caused by the use of toxic crosslinking agents are avoided, the biocompatibility is good, the loaded transplanted cells can inhibit the rejection reaction after cell transplantation to a certain extent, the survival time of the transplanted material is obviously prolonged, and the conduit material adopts western Chinese milk vetch glue, which can be used as a retarder for controlled release preparation and can adjust the release of the loaded drug. BRIEF DESCRIPTION OF DRAWINGS

[0032] Figure 1 The longitudinal section scanning electron microscope image of the laminarin-silk floss medical conduit of western Chinese milk vetch glue prepared by the present application;

[0033] Figure 2 Structure diagram of the laminarin-astrogal- silk microfiber medical catheter prepared by the present application;

[0034] Figure 3 Structure diagram of the catheter mold used in the laminarin-astrogal- silk microfiber medical catheter prepared by the present application.

[0035] Legend of reference signs:

[0036] 1, outer tube; 2, inner core; 3, lower plugging; 4, upper pressing head; 5, opening. DETAILED DESCRIPTION

[0037] In order to make the technical problems, technical solutions and advantages of the present application more clear, the following will be described in detail with reference to the drawings and specific embodiments.

[0038] As shown in Figure 1 , 2 , the embodiment of the present application provides a laminarin-astrogal-silk microfiber medical catheter, wherein Figure 1 (a) is a scanning electron microscope image of the laminarin-astrogal-silk microfiber medical catheter cross section, Figure 1 (b) is Figure 1 (a) is a local enlarged view of the square in (a), Figure 1 (c) is Figure 1 (b) is a local enlarged view of the square in (b). It can be clearly seen from the figure that the laminarin-astrogal-silk microfiber medical catheter has a rich three-dimensional structure, which also provides the necessary way for the transport of nutrients required in various processes of transplanted cells, growth factors, drug molecules, etc., and provides the necessary induction and necessary growth space for the growth of various transplanted cells. Each 100ml of medical catheter preparation solution contains the following components: laminarin 1-10g, aqueous solution 10-100ml, 10% astrogal solution 2-30ml, silk microfiber 1-10g.

[0039] The 10% astrogal solution is prepared from astrogal and pure water, and the mass percentage of astrogal and pure water is 1:9.

[0040] The preparation method of the silk microfiber is the silk microfiber preparation method in “CN201710373332-silk microfiber-gelatin-polyethylene glycol medical catheter and its preparation method”, that is, it is divided into three stages:

[0041] a. preparing degummed silk fibers from cocoons;

[0042] b. hydrolyzing the degummed silk fibers into micron-sized fibers;

[0043] c. Washing / neutralization and freeze-drying of the fibers.

[0044] The embodiment of the present application also provides a preparation method of laminarin-scutellaria baicalensis georgi-silk microfiber medical catheter, comprising the following steps:

[0045] S1, preparing a silk microfiber solution: taking a formula amount of silk microfiber and dissolving it in 10-100 mL of aqueous solution, heating and stirring to completely dissolve it;

[0046] S2, weighing a formula amount of 10% scutellaria baicalensis georgi solution, slowly pouring the 10% scutellaria baicalensis georgi solution into the silk microfiber solution prepared in step S1, and fully stirring and dissolving with a glass rod;

[0047] S3, weighing a formula amount of laminarin, adding it to the mixture obtained in step S2, fully stirring and dissolving, and using intermittent sound pressure 0.05 MPa pulse ultrasound for 10 min / time, a total of 3 times, with 5 min intervals each time, which can increase the porosity of the catheter;

[0048] S4, using a 10 ml syringe to draw the medical catheter preparation solution of step S3, injecting it into the catheter mold, fixing the catheter mold containing the medical catheter preparation solution, and placing it on a glass dish in the refrigerator and freezing overnight;

[0049] S5, taking out the catheter mold in the refrigerator in step S4, removing the outer layer of the catheter mold, forming a catheter mold wrapped around the inner core, clamping the catheter mold wrapped around the inner core into a freeze-drying bottle with tweezers, covering the bottle stopper, and placing it horizontally into the refrigerator and freezing;

[0050] S6, taking out the freeze-dried bottle after freezing in step S5, completely freeze-drying the freeze-dried bottle with a freeze-drying machine, taking the freeze-dried bottle from the freeze-drying machine and taking out the catheter mold wrapped around the inner core, carefully removing the inner core in the catheter mold, and obtaining a laminarin-scutellaria baicalensis georgi-silk microfiber medical catheter;

[0051] S7, placing the prepared laminarin-scutellaria baicalensis georgi-silk microfiber medical catheter into a sealed bag, marking the name and preparation time, and storing it in a cool and dry place for a long time, taking out the catheter for Co60 irradiation and disinfection before use, and standby.

[0052] The catheter mold used in the preparation of the laminarin-scutellaria baicalensis georgi-silk microfiber medical catheter is as follows Figure 3As shown, the catheter mold comprises an outer tube 1, an inner core 2, a lower plug 3 and an upper pressure head 4. The lower plug 3 is in the shape of an inverted T, and has a lower plug central hole with an open upper end and a closed lower end. The lower end of the inner core 2 is inserted into the lower plug central hole, and the upper end of the inner core 2 penetrates into the lower end of the outer tube 1 and extends above the upper end of the outer tube 1. The upper part of the lower plug 3 is inserted into the lumen of the outer tube 1, and the lower part is located below the outer tube 1.

[0053] The upper pressure head 4 is in the shape of a T, and has an upper pressure head central hole with open upper and lower ends. The upper pressure head is sleeved between the outer tube 1 and the inner core 2, and the lower part is inserted into the lumen of the outer tube 1, and the upper part is located above the outer tube 1. The gap between the upper pressure head 4 and the lower plug 3 is a medical catheter preparation solution filling area. The outer tube 1 is also provided with an opening 5 for injecting the medical catheter preparation solution. The medical catheter preparation solution is injected into the catheter preparation solution filling area along the opening 5. After the above steps, the laminarin-western milk vetch glue-silk microfiber medical catheter is finally formed.

[0054] The technical solutions of the present application are further described below in combination with several specific embodiments.

[0055] Embodiment one,

[0056] Preparation of sample P1:

[0057] S1, prepare a silk microfiber solution: dissolve 6g of silk microfiber in 60mL of water solution, and heat and stir until completely dissolved;

[0058] S2, weigh 20ml of 10% western milk vetch glue solution, and slowly pour the solution into the silk microfiber solution prepared in step S1, and fully stir and dissolve with a glass rod;

[0059] S3, weigh 5g of laminarin, and add it to the mixed solution prepared in step s2, and ultrasonically stir at 70℃ for 2.5h to fully dissolve it, and use intermittent sound pressure pulse ultrasonic action at 0.05MPa for 10min each time, for a total of 3 times, with 5min of intermittent time each time;

[0060] S4, use a 10ml syringe to draw the medical catheter preparation solution of step S3, and inject it into the catheter mold. Fix the catheter mold containing the medical catheter preparation solution, and place it on a glass dish, and put it in a -20℃ refrigerator and freeze overnight;

[0061] S5, take out the catheter mold in the refrigerator of step S4, remove the outer layer of the catheter mold, form a catheter mold wrapping the inner core, and use tweezers to clamp the catheter mold wrapping the inner core into a freeze-drying bottle, cover the bottle stopper, and horizontally place it in the refrigerator and freeze at -20℃ for 2h;

[0062] S6, open the freeze dryer, when the temperature is reduced to below -40℃ and the vacuum is reduced to 0.02Pa, take out the freeze-dried bottle after freezing in step S5, move the freeze-dried bottle to the freeze dryer and make it communicate with the vacuum pump, the freeze-drying time is 12h, after the freeze dryer completely freeze-dries the freeze-dried bottle, take down the freeze-dried bottle and take out the catheter mold wrapped in the core, carefully remove the core in the catheter mold, and get the laminarin-western mulberry root-silk microfiber medical catheter;

[0063] S7, put the prepared laminarin-western mulberry root-silk microfiber medical catheter into a sealed bag, mark the name and preparation time, and store it in a cool and dry place for a long time. Before use, take out the catheter, irradiate it with Co60, disinfect it, and use it.

[0064] Example two:

[0065] Preparation of sample P2:

[0066] S1, prepare a silk microfiber solution: dissolve 2g of silk microfiber in 80mL of aqueous solution, heat and stir until completely dissolved;

[0067] S2, weigh 20ml of 10% western mulberry root solution, slowly pour the solution into the silk microfiber solution prepared in step S1, and stir with a glass rod until dissolved;

[0068] S3, weigh 1g of laminarin, add to the mixture prepared in step s2, and ultrasonically stir at 70℃ for 2.5h to make it fully dissolved, and use intermittent sound pressure 0.05MPa pulse ultrasonic action for 10min / time, a total of 3 times, with 5min interval each time;

[0069] S4, use a 10ml syringe to draw the medical catheter preparation solution in step S3, inject it into the catheter mold, fix the catheter mold containing the medical catheter preparation solution, and place it on a glass dish, and put it in a-20℃ refrigerator and freeze overnight;

[0070] S5, take out the catheter mold in the refrigerator in step S4, remove the outer layer of the catheter mold to form a catheter mold wrapped in the core, clamp the catheter mold wrapped in the core with tweezers into a freeze-dried bottle, cover the bottle stopper, and place it horizontally in the refrigerator and freeze at-20℃ for 2h;

[0071] S6, open the freeze dryer, when the temperature is reduced to below -40℃ and the vacuum is reduced to 0.02Pa, take out the freeze-dried bottle after freezing in step S5, move the freeze-dried bottle to the freeze dryer and make it communicate with the vacuum pump, the freeze-drying time is 12h, after the freeze dryer completely freeze-dries the freeze-dried bottle, take down the freeze-dried bottle and take out the catheter mold wrapped in the core, carefully remove the core in the catheter mold, and get the laminarin-western mulberry root-silk microfiber medical catheter;

[0072] S7, the prepared laminarin-western milkvetch gum-silk microfiber medical catheter is placed in a sealed bag, and the name and preparation time are marked. It can be stored for a long time in a cool and dry place. Before use, the catheter is taken out and irradiated with Co60 for sterilization, and is ready for use.

[0073] Example Three,

[0074] Preparation of sample P3:

[0075] S1, prepare a silk microfiber solution: dissolve 4g of silk microfiber in 40ml of water solution, heat and stir until completely dissolved;

[0076] S2, weigh 8ml of 10% western milkvetch gum solution, slowly pour the solution into the silk microfiber solution prepared in step S1, and stir with a glass rod until dissolved;

[0077] S3, weigh 3g of laminarin, add to the mixture prepared in step s2, and stir at 70°C for 2.5h to make it fully dissolved. Pulse ultrasonic treatment is used with an intermittent sound pressure of 0.05MPa, 10min / time, 3 times, with 5min intervals each time;

[0078] S4, use a 10ml syringe to draw the medical catheter preparation solution of step S3, and inject it into the catheter mold. Fix the catheter mold containing the medical catheter preparation solution, and place it on a glass dish. Place it in a-20°C refrigerator and freeze overnight;

[0079] S5, take out the catheter mold in the refrigerator of step S4, remove the outer layer of the catheter mold, form a catheter mold wrapped around the inner core, and clamp the catheter mold wrapped around the inner core into a freeze-drying bottle with tweezers. Close the bottle stopper and place it horizontally in the refrigerator at-20°C for 2h;

[0080] S6, open the freeze-drying machine, and when the temperature drops to below-40°C and the vacuum drops to 0.02Pa, take out the frozen freeze-drying bottle of step S5. Connect the freeze-drying bottle to the vacuum pump, and freeze-dry for 12h. After the freeze-drying machine completely freeze-dries the freeze-drying bottle, take it out and remove the inner core of the catheter mold. Laminarin-western milkvetch gum-silk microfiber medical catheter is obtained;

[0081] S7, the prepared laminarin-western milkvetch gum-silk microfiber medical catheter is placed in a sealed bag, and the name and preparation time are marked. It can be stored for a long time in a cool and dry place. Before use, the catheter is taken out and irradiated with Co60 for sterilization, and is ready for use.

[0082] The porosity and tensile load of samples P1, P2 and P3 are detected respectively, and the detection results are shown in Table 1.

[0083] Sample Porosity Maximum load P1 >65％ 6.234N P2 Poor molding 0.973N P3 <40％ 4.062N .

[0084] From table 1, sample P1 has good porosity, mechanical strength and good flexibility, better meet the requirements of cell transplantation and cell product, drug delivery and other research fields, the added radix astragali gum can be used as a retarder of controlled release preparation, and the release of the drug is adjusted, which is a good medical biomimetic catheter for tissue engineering.

[0085] The beneficial effects of the above technical solutions of the present application are as follows:

[0086] (1) The formula raw material of the present application has wide sources and low price, and has the functions of medical polymer material surface adsorption, interception and grafting.

[0087] (2) The medical catheter prepared by the formula of the present application is non-toxic and immunologically inert, and improves the biocompatibility of medical polymer materials in contact with blood.

[0088] (3) The catheter prepared by the formula of the present application has a rich three-dimensional structure, and adopts low-intensity pulse ultrasonic process, through ultrasonic cavitation effect, the porosity is greater than 65%, which can provide necessary way for the transport of nutrients required by various transplanted cells, growth factors, drug molecules and other processes, and provide necessary induction and growth space for the growth of various transplanted cells.

[0089] (4) The present application can load transplanted cells and inhibit the rejection reaction after cell transplantation to a certain extent, and significantly prolong the survival time of the transplanted cells.

[0090] (5) The stent material of the present application can be used as a retarder of controlled release preparation, and can adjust the release of the loaded drug.

[0091] (6) The gel medical stent obtained by the preparation method of the present application has good mechanical strength, and the maximum load is 6.234N.

[0092] The above is the preferred embodiment of the present application, it should be pointed out that for ordinary skilled in the art, without departing from the principles of the present application, can make a number of improvements and refinements, these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A Laminarin- Astragalus Gum- Silk Microfiber Medical Catheter, characterized in that, The medical catheter preparation solution contains the following components per 100ml: laminarin 3-10g, water solution 20-80ml, 10% radix astragali gum solution 10-25ml, silk microfiber 3-7g; The preparation method of the laminarin-radix astragali gum-silk microfiber medical catheter comprises the following steps: S1, preparing a silk microfiber solution: dissolve the formula amount of silk microfiber in 20-80ml of water solution, heat and stir to completely dissolve; S2, weigh the formula amount of 10% radix astragali gum solution, slowly pour the 10% radix astragali gum solution into the silk microfiber solution prepared in step S1, and fully stir and dissolve with a glass rod; S3, weigh the formula amount of laminarin, add it to the mixture obtained in step S2, fully stir and dissolve, and use intermittent pulse ultrasonic with a sound pressure of 0.05MPa for 10min / time, for a total of 3 times, with an interval of 5min each time; S4, use a 10ml syringe to draw the medical catheter preparation solution of step S3 and inject it into the catheter mold, fix the catheter mold containing the medical catheter preparation solution, and place it on a glass dish in the refrigerator overnight; S5, take out the catheter mold in the refrigerator of step S4, remove the outer layer of the catheter mold, form a catheter mold wrapped around the inner core, clamp the catheter mold wrapped around the inner core into a freeze-drying bottle with tweezers, cover the bottle stopper, and place it horizontally in the refrigerator for freezing; S6, take out the freeze-dried bottle after freezing in step S5, completely freeze-dry the freeze-dried bottle with a freeze-drying machine, take the freeze-dried bottle from the freeze-drying machine, and take out the catheter mold wrapped around the inner core, carefully remove the inner core in the catheter mold, and obtain the laminarin-radix astragali gum-silk microfiber medical catheter; S7, place the prepared laminarin-radix astragali gum-silk microfiber medical catheter in a sealed bag, mark the name and preparation time, and store it in a cool and dry place for long-term storage, irradiate and sterilize the catheter with Co60 before use, and prepare for use; The 10% radix astragali gum solution is prepared from radix astragali gum and pure water, and the mass percentage of radix astragali gum and pure water is 1:9; The medical catheter is a hollow circular tube mesh stent in the middle, and the mesh stent is loaded with a mixed solution of laminarin-radix astragali gum-silk microfiber.

2. The Laminarin-Astragalus Gum-Silkworm Silk Microfiber Medical Catheter according to claim 1, characterized in that, The medical catheter preparation solution contains the following components per 100ml: laminarin 5g, water solution 60ml, 10% radix astragali gum solution 20ml, and silk microfiber 6g.

3. The Laminarin-Astragalus Gum-Silkworm Silk Microfiber Medical Catheter according to claim 1, characterized in that, The preparation method of the laminarin-radix astragali gum-silk microfiber medical catheter comprises the following steps: S1, preparing a silk microfiber solution: dissolve 6g of silk microfiber in 60ml of water solution, heat and stir to completely dissolve; S2, weigh 20ml of 10% radix astragali gum solution, slowly pour the solution into the silk microfiber solution prepared in step S1, and fully stir and dissolve with a glass rod; S3, weigh 5g of laminarin, add it to the mixture prepared in step S2, and stir at 70℃ for 2.5h to fully dissolve, and use intermittent pulse ultrasonic with a sound pressure of 0.05MPa for 10min / time, for a total of 3 times, with an interval of 5min each time, which can increase the porosity of the catheter; S4, using 10 ml syringe to suck the medical catheter preparation solution of step S3, inject into the catheter mold, fix the catheter mold containing the medical catheter preparation solution, place it in a glass dish, and place it in a-20℃ refrigerator and freeze overnight; S5, take out the catheter mold in the refrigerator of step S4, remove the outer layer of the catheter mold, form a catheter mold wrapped around the inner core, clamp the catheter mold wrapped around the inner core into a freeze-dried bottle with tweezers, cover the bottle plug, and place it horizontally in the refrigerator and freeze at-20℃ for 2h; S6, open the freeze-drier, when the temperature drops to below-40℃ and the vacuum drops to 0.02Pa, take out the freeze-dried bottle after freezing in step S5, move the freeze-dried bottle to the freeze-drier and make it communicate with the vacuum pump, the freeze-drying time is 12h, after the freeze-drier completely freeze-dries the freeze-dried bottle, take out the freeze-dried bottle and take out the catheter mold wrapped around the inner core, carefully remove the inner core in the catheter mold, and get the laminarin-silk floss-scutellaria baicalensis georgi medical catheter; S7, put the prepared laminarin-silk floss-scutellaria baicalensis georgi medical catheter into a sealed bag, mark the name and preparation time, and store it in a cool and dry place for a long time. Before use, take out the catheter and sterilize it with Co60, and it is ready for use.

4. The Laminarin-Astragalus Gum-Silk Fibroin Microfiber Medical Catheter according to claim 1, characterized in that, The catheter mold comprises an outer tube, an inner core, a lower stopper and an upper pressure head. The lower stopper is in inverted T-shaped structure, and a lower stopper center hole with an open upper end and a closed lower end is arranged in the lower stopper. The lower end of the inner core is inserted into the lower stopper center hole, and the upper end of the inner core is inserted into the lower end of the outer tube and extends above the upper end of the outer tube. The upper part of the lower stopper is inserted into the lumen of the outer tube, and the lower part is limited below the outer tube. The upper pressure head is in T-shaped structure, and an upper pressure head center hole with open upper and lower ends is arranged in the upper pressure head. The upper pressure head is sleeved between the outer tube and the inner core, and the lower part is inserted into the lumen of the outer tube and the upper part is limited above the outer tube. The gap between the upper pressure head and the lower stopper is the medical catheter preparation solution filling area. The outer tube body is also provided with an opening hole for injecting the medical catheter preparation solution, and the medical catheter preparation solution is injected into the catheter preparation solution filling area along the opening hole.

Images