A skin care composition and use for improving skin redness, dryness issues

Abstract

The application discloses a skin care composition for improving skin redness and dryness and application. The composition of the application is compounded by hyaluronic acid lysine copper chelate, radix stephaniae tetrandrae extract and polygonatum extract. The composition can promote the expression of moisture retention related factors HAS1 and AQP3, and inhibit the expression of inflammation related factors PGE2 and TNF-alpha, so as to improve the skin redness and dryness. The composition is used in acid mud film, and the stability experiment shows that the composition can maintain the stability of the mud film product under low pH conditions, and the appearance is uniform. It is also found that the composition can greatly reduce the irritation of the acid mud film.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, in particular to a skin care composition for improving skin redness and dryness and application. BACKGROUND

[0002] In recent years, the incidence of sensitive skin (SS) has gradually increased, seriously affecting people's quality of life, and has become a global health problem that has attracted much attention. According to the International Forum for the Study of Itch (IFSI) and the Chinese Expert Consensus on the Diagnosis and Treatment of Sensitive Skin (2017 version), sensitive skin is defined as a syndrome in which the skin appears to be paroxysmal or periodic burning, paroxysmal redness, stinging, itching and tightness after being stimulated by external stimuli, with or without persistent erythema. Sensitive skin is most likely to occur on the face. Current research suggests that the occurrence of sensitive skin is a complex process involving skin barrier, neurovascular response and innate immune inflammation. The main cause is the impairment of skin barrier function, increased transepidermal water loss, decreased water content in the stratum corneum, and decreased moisturizing and occlusive ability, leading to dry skin and enhanced penetration of irritants or allergens. At the same time, in terms of immune inflammation, immune cells release pro-inflammatory cytokines or inflammatory mediators such as PEG-2 and TNF-α in response to stimuli, causing symptoms such as skin redness, itching, burning and stinging. Therefore, the care of sensitive skin must repair the damaged skin barrier while reducing the release of skin inflammatory cytokines.

[0003] The formation and development mechanism of sensitive skin is complex, making it extremely difficult to repair and improve sensitive skin. Many products on the market can only temporarily relieve skin sensitivity, and do not have a deep repair effect on sensitive skin. Moreover, most existing soothing and repairing compositions are formulated by adding a large number of soothing and repairing ingredients, which are complex in composition, have unclear mechanisms of action, are high in cost, and have no significant effect. Therefore, it is necessary to develop a product that is simple in composition, mild and non-irritating, has good soothing effect, and improves skin redness and dryness. SUMMARY

[0004] In view of the above problems, the present application provides a skin care composition for improving skin redness and dryness and application. The composition of the present application is compounded from hyaluronic acid lysine copper chelate, radix stephaniae tetrandrae extract and polygonatum extract. Through experimental verification, the composition has good soothing and moisturizing effects, and can be used in cosmetics to improve skin redness and dryness, and has good application prospects in cosmetics.

[0005] In order to achieve the above technical purposes, the technical scheme adopted by the present application is as follows: a skin care composition for improving skin redness and dryness problems, characterized in that the effective components are hyaluronic acid lysine copper chelate, Stephania tetrandra extract and polygonatum extract, and the mass fractions of the effective components are: 0.1-1.0 parts of hyaluronic acid lysine copper chelate, 1.0-15.0 parts of Stephania tetrandra extract, and 1.0-15.0 parts of polygonatum extract.

[0006] The composition also includes a solvent, butanediol and water.

[0007] Preferably, the components and mass fractions are: 0.5-1.0 parts of hyaluronic acid lysine copper chelate, 5.0-10.0 parts of Stephania tetrandra extract, 5.0-10.0 parts of polygonatum extract, 12.0-18.0 parts of butanediol, and water to make up 100 parts.

[0008] Preparation method: mix the polygonatum extract, Stephania tetrandra extract and butanediol together, then add the hyaluronic acid lysine copper chelate, and dissolve in purified water to make up 100 parts.

[0009] The hyaluronic acid lysine copper chelate used in the present application can be commercially available or obtained by a salt formation method (application number: CN202410478415.3). The hyaluronic acid lysine copper chelate used in the examples of the present application is obtained by example 1 of CN202410478415.3.

[0010] The method for obtaining the Stephania tetrandra extract is commercially available or the following method: a suitable amount of dried Stephania tetrandra powder is extracted by an ultrasonic-assisted low eutectic solvent extraction method, the extract is filtered, and the filtrate is concentrated and then butanediol is added to obtain the extract. The concentration is 0.3-0.8 g / mL of crude drug, that is, 0.3-0.8 g of dried Stephania tetrandra powder to obtain 1 mL of Stephania tetrandra extract (solution).

[0011] Further, the specific steps are as follows:

[0012] (1) The low eutectic solvent is prepared by an oil bath heating and stirring method, the hydrogen bond acceptor betaine and the hydrogen bond donor 1,2-propanediol are heated and stirred at 75-85℃ according to a molar ratio of 1:2 for 2-6h until a clear and uniform solution is formed, and then the solution is placed in a vacuum drying box with a temperature higher than the melting point value for drying. In order to reduce the viscosity, 2-5 times the mass of water is added to the solution.

[0013] (2) Take an appropriate amount of powder of Stephania tetrandra powder into the eutectic solvent prepared in step (1), the solid-liquid ratio is 1:10-1:15, ultrasonic extraction at room temperature under the ultrasonic power of 200-300W for 30-60min, after extraction, filter, the filter residue is repeatedly extracted for 1 time, the solid-liquid ratio of the second extraction is 1:8-1:10, the extraction time is 20-40min, after the two filtrates are combined, filter;

[0014] (3) The filtrate prepared in step (2) is concentrated at 65-85℃ under reduced pressure, the same mass of butanediol as the concentrated solution is added, stirred and mixed uniformly, filtered to obtain the Stephania tetrandra extract.

[0015] The method for obtaining the extract of Polygonatum sibiricum is obtained by using the following method: an appropriate amount of dried Polygonatum sibiricum powder is extracted by supercritical fluid extraction, the extract is filtered, and glycerol is added after the filtrate is concentrated to obtain the extract. The concentration is 0.4-1.0g / mL of crude drug, that is, 0.4-1.0g of dried Polygonatum sibiricum powder to obtain 1mL of Polygonatum sibiricum extract (solution).

[0016] Further, the specific steps are as follows:

[0017] (1) Take an appropriate amount of dried Polygonatum sibiricum powder into the extractor, set the temperature to 50-60℃, the pressure to 20-40MPa, the flow rate of 60%-80% ethanol entrainer to 0.3-0.5mL / min, and the flow rate of CO2 to 1.5-3.0mL / min, and then extract and collect for 2-4h after the parameters are stable, and filter the collected filtrate;

[0018] (2) The filtrate prepared in step (1) is concentrated at 55-70℃ under reduced pressure, 1-2 times the mass of glycerol is added to the concentrated solution, stirred and mixed uniformly, and then filtered to obtain the extract of Polygonatum sibiricum.

[0019] Hyaluronic acid lysine copper chelate has high moisturizing properties and better skin adhesion, has a significant inhibitory effect on 5α-reductase, can effectively control oil, prevent and treat acne vulgaris, improve the water-oil balance of the skin, prevent seborrheic alopecia and improve the quality of the hair, repair skin and mucosal damage, promote the generation of elastin and collagen, prevent and reduce skin wrinkles, make the skin smooth and elastic, have the effect of anti-wrinkle and firming, and improve facial aging.

[0020] Stephania tetrandra is a natural ingredient with multiple skin care effects, mainly including anti-inflammatory, anti-allergic, anti-aging and other properties. It is derived from the dried roots of Stephania tetrandra, and has wide medical and cosmetic applications.

[0021] Polygonatum sibiricum is a commonly used traditional Chinese medicine, which is rich in active ingredients such as polygonatin and baicalin, and can improve skin problems through antioxidant and heat-clearing and detoxifying ways.

[0022] In a second aspect of the present application, the use of the above-mentioned composition in the preparation of a skin care cosmetic for improving redness and dryness of the skin is provided. The above-mentioned composition can promote the expression of the moisture-related factors HAS1 (hyaluronic acid synthase 1 gene) and AQP3 and inhibit the expression of the inflammation-related factors PGE2 and TNF-α, thereby achieving the effects of soothing and moisturizing, and is safe and non-toxic, and thus can be applied to skin care cosmetics for improving redness and dryness of the skin.

[0023] In a third aspect of the present application, a skin care cosmetic for improving redness and dryness of the skin is provided, which comprises the above-mentioned skin care composition for improving redness and dryness of the skin.

[0024] Further, the amount of the composition included in the total mass of the skin care cosmetic for improving redness and dryness of the skin is 1.0-10.0%. More preferably, the amount of the composition included is 3.0-8.0%.

[0025] Further, the skin care cosmetic for improving redness and dryness of the skin further comprises one or a combination of two or more of an emulsifier, an emollient, a humectant, a rheology modifier, and a preservative.

[0026] Meanwhile, the present application can be used to prepare different cosmetic dosage forms such as serum, essence, mask, emulsion, and cream by reasonably adding the above-mentioned raw material components, and based on the above-mentioned basic cosmetic categories, further derived cosmetic categories are obtained, which are obviously within the protection scope of the present application.

[0027] Advantages of the present application:

[0028] (1) The present application combines hyaluronic acid lysine copper chelate, radix stephaniae tetrandrae extract, and polygonatum extract, and the three components synergistically promote the expression of the moisture-related factors HAS1 (hyaluronic acid synthase 1 gene) and AQP3 and inhibit the expression of the inflammation-related factors PGE2 and TNF-α, thereby achieving the effect of improving redness and dryness of the skin, and the effect of improving redness and dryness of the skin in vitro is good.

[0029] (2) The composition of the present application is used in an acid mud mask, and stability experiments show that the composition can maintain the stability of the mud mask product under low pH conditions, and the appearance is uniform, and it is also found that the composition can greatly reduce the irritation of the acid mud mask. DETAILED DESCRIPTION

[0030] The application is further illustrated by the following examples without limiting the application to the examples. Any variation of the application based on the examples without creative work is within the protection scope of the application. In the examples, all raw materials are commercially available unless otherwise specified.

[0031] Examples 1-4 and Comparative Examples 1-6:

[0032] The raw material ratio of Examples 1-4 and Comparative Examples 1-6 is shown in Table 1 below, calculated by mass fraction. The preparation method is as follows: mix the polygonatum extract, the stephania tetrandra extract and butanediol together, then add the hyaluronic acid lysine copper chelate, and dissolve in purified water to make up 100 parts.

[0033] Table 1: Ingredient list of specific examples and comparative examples

[0034]

[0035] The hyaluronic acid lysine copper chelate used in the examples of the application is obtained by Example 1 of CN202410478415.3. Specifically, after the sodium hyaluronate and the lysine copper hydrochloride chelate are mixed and dissolved uniformly, the catalyst EDCI and NHS are added, and after the reaction is kept warm, an appropriate amount of ethanol is added for precipitation and purification, filtration, dehydration with anhydrous ethanol, and then reduced pressure drying to obtain blue granular powder, samples 1-6.

[0036] The stephania tetrandra extract is obtained by the following method:

[0037] (1) Prepare a deep eutectic solvent: mix betaine and 1,2-propanediol at a molar ratio of 1:2, heat and stir at 80℃ for 4.5h until a clear and uniform solution is formed, then place the solution in a vacuum drying oven at a temperature higher than the melting point value for 24h, and add 3 times the mass of water to the solution to reduce the viscosity;

[0038] (2) Take an appropriate amount of stephania tetrandra powder and place it in the deep eutectic solvent prepared in step (1), with a solid-liquid ratio of 1:12, and ultrasonically extract at 250W for 45min at room temperature. After extraction, filter the filtrate, and repeat the extraction once with the filtrate. The second extraction is performed at a solid-liquid ratio of 1:8, and the extraction time is 30min. After combining the two filtrates, filter them;

[0039] (3) Concentrate the filtrate obtained in step (2) at 65-85℃ under reduced pressure, add butanediol of the same mass as the concentrated solution, stir and mix uniformly, and filter to obtain the stephania tetrandra extract. The concentration is 0.35g / mL, i.e. 0.35g of dried stephania tetrandra powder, to obtain 1mL of stephania tetrandra extract (solution).

[0040] The Huangqi extract is obtained by the following method:

[0041] (1) A suitable amount of dried Huangqi powder is placed in a supercritical fluid extractor, and the temperature is set to 55°C, the pressure is set to 25 MPa, the flow rate of 65% ethanol entrainer is set to 0.35 mL / min, and the flow rate of CO2 is set to 2.0 mL / min. After the parameters are stabilized, extraction is performed for 3 hours, and the filtrate is collected and filtered.

[0042] (2) The filtrate obtained in step (1) is concentrated at 55-70°C under reduced pressure, 2 times the mass of glycerol is added to the concentrated solution, and the mixture is stirred until uniform. After filtration, the Huangqi extract is obtained. The concentration is 0.45 g / mL based on crude drug, i.e., 0.45 g of dried Huangqi powder, to obtain 1 mL of Huangqi extract (solution).

[0043] Example 5: Preparation of a brush acid mud film

[0044] A brush acid mud film product containing a skin care composition for improving skin redness and dryness problems is prepared, and the formula is shown in Table 2.

[0045] Table 2: Formula of the brush acid mud film product

[0046]

[0047] Preparation method of the brush acid mud film:

[0048] S1: Preparation of the water phase: The raw materials of phase A are added to an emulsifying tank and stirred to dissolve; the raw materials of phase B are separately dispersed and then added to the main pot and stirred to dissolve; the raw materials of phase C are added to the emulsifying tank in sequence and dispersed until there are no particles, and then heated to 80-85°C;

[0049] S2: Preparation of the oil phase: The raw materials of phase D are added to the oil phase and heated to 75-80°C, and then stirred to dissolve;

[0050] S3: Emulsification: The dissolved raw materials of phase D are added to the emulsifying tank for emulsification and homogenization, the homogenization speed is 6000 rpm, the time is 10 min, and the temperature is lowered;

[0051] S4: The temperature is lowered to 45°C, phase E (which is heated to 70°C in a separate clean container and stirred to dissolve) and phase F are added and stirred to mix uniformly, and the brush acid mud film is prepared.

[0052] The pH value of the product is in the range of 4.4-5.5. The composition can maintain the stability of the mud film product and the appearance is uniform.

[0053] The preparation method of Comparative Example 7 is the same as that of Example 5, and Comparative Example 7 does not add the skin care composition for improving skin redness and dryness problems.

[0054] Test Example 1: Stability investigation test

[0055] Examples 1-4 and Comparative Examples 1-6 were placed at room temperature, -18°C, 4°C, 45°C, and subjected to cold-heat cycling (-18°C, 4°C, 45°C, cycled once every 3 days) for a corresponding period of time, and the stability was observed to determine whether precipitation or discoloration occurred. If no precipitation or discoloration occurred, the stability passed. The results are shown in Table 3 below.

[0056] Table 3 Stability test results

[0057]

[0058] Note: √ means that the stability passed and no precipitation occurred; × means that the solution had slight precipitation; ×× means that the solution had severe precipitation; ○ means that the solution color changed and the color slightly darkened; and ○○ means that the solution color changed and the color darkened severely.

[0059] As shown in Table 3, when the hyaluronic acid lysine copper chelate, the polygonatum extract, and the radix Stephania tetrandra extract were all present, the solution stability was normal and passed the stability test. When any one or two of the three were present, precipitation or discoloration occurred and the stability test failed, except for Comparative Example 1.

[0060] Test Example 2: Irritation test

[0061] The product was subjected to an eye irritation test, i.e., a safety test, according to SN / T 2329-2009 "Cosmetic Eye Irritation / Chicken Embryo Chorioallantoic Membrane Test".

[0062] Experimental method: 7-day-old fertilized chicken embryos were incubated in an incubator at 37.6±0.5°C and 50%-70% humidity for 9 days.

[0063] CAM preparation: The eggs were checked for candling, the air chamber position was marked on the eggshell surface, the marked eggshell portion was removed with tweezers, the white egg membrane was exposed, and the operation was performed carefully to not damage the integrity of the egg membrane. 0.5 mL of 0.9% NaCl solution was added to fully wet the egg membrane, the surface liquid was gently absorbed with a paper towel, and the inner membrane was carefully removed with tweezers to ensure that the blood vessel membrane was not damaged.

[0064] Experimental method: 0.3 mL of the liquid was directly applied to the CAM to ensure that 50% of the CAM surface was covered with the test substance. After 3 min, the test substance on the CAM was gently rinsed with physiological saline, and the results were observed 30 s after the rinsing was completed. Six chicken embryos were used for each test substance (Examples 5 and Comparative Example 7), and one chicken embryo was used for the negative control (physiological saline) and the positive control (1% SDS).

[0065] End point score (ES): The test performed by the reaction end point method should calculate the end point score (ES), and the result is retained to two decimal places. The score of each embryo = the sum of the observed degree of bleeding, coagulation and vascular resolution of each embryo, ES = the mathematical sum of the scores of 6 embryos. According to the ES value, the eye irritation of the test substance is classified according to Table 4.

[0066] Table 4 Judgment criteria for reaction end point method results

[0067] Irritation score ES < 12 12 < ES < 16 ES > 16 Irritation classification Non / mildly irritating Moderately irritating Strongly irritating / corrosive

[0068] Table 5 Test quality control standards and results

[0069]

[0070] The test conforms to the test quality control standards, and the results are acceptable.

[0071] The test samples of Example 5 and Comparative Example 7 were diluted with water to 1% solution for experimental testing. The irritation experiment result statistics are shown in Table 6. The composition of the application can reduce the irritation of the acid peel mask to the skin and reduce the allergic irritation problem of using the product.

[0072] Table 6 Irritation experiment result statistics table

[0073] Reaction endpoint method ES value Result Example 5 0.00 Non-irritating Comparative Example 7 11.00 Mildly irritating

[0074] Test Example 2: In vitro moisturizing efficacy test

[0075] AQP3 detection experiment: In this test, keratinocytes were selected as the test model. After the cells were resuscitated and plated at a rate of about 60%, the cells were inoculated into a 24-well plate and incubated in a CO2 incubator (37°C, 5% CO2) overnight. The test substance working solution was prepared according to Table 7. Each group was given medication, and each group had 3 replicate wells. The blank control group was added with 1 mL of culture medium (KcGrowth culture medium (Guangdong Boxi Biological)) per well, the positive control group was added with 1 mL of culture medium containing CaCl2 per well, and the sample group was added with 1 mL of culture medium containing the corresponding concentration of the sample to be tested per well. After the medication was completed, the 24-well plate was placed in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0076] Immunofluorescence test: fixed with 4% paraformaldehyde, after 24 h of fixation, immunofluorescence detection was performed, and the pictures were observed under a microscope, collected and analyzed.

[0077] HAS1 gene detection experiment: The keratinocytes were selected as the test model. After the cells were recovered and plated at a rate of about 60%, the cells were inoculated into a 24-well plate and incubated in a CO2 incubator (37°C, 5% CO2) overnight. The test working solution was prepared according to Table 7. Each group was administered, and each group had three replicate wells. The blank control group was added with 2 mL of culture medium per well, the PC2 group was added with 2 mL of culture medium containing TGF-β1 per well, and the sample group was added with 2 mL of culture medium containing the corresponding concentration of the sample to be tested per well. After the administration was completed, the plate was placed in a CO2 incubator (37°C, 5% CO2) for 24 h. After incubation, the old liquid was aspirated, washed twice with PBS, 1 mL of RNAiso Plus was added to each well, the cells were lysed by blowing, and the sample was collected. After the RNA was extracted and reverse transcribed into cDNA, fluorescence quantitative PCR detection was performed, and 2 -△△CT Method for calculating results.

[0078] GraphPad Prism was used for plotting, and the results were expressed as Mean ± SD. t-test statistical analysis was used for comparison between groups. The statistical analysis was two-tailed. P < 0.05 was considered to have a significant difference, and P < 0.01 was considered to have a very significant difference.

[0079] Table 7 Grouping of in vitro moisturizing efficacy test experiment

[0080]

[0081] The test results are shown in Tables 8 and 9, respectively.

[0082] Table 8 AQP3 immunofluorescence analysis result summary table

[0083]

[0084]

[0085] Note: Integrated optical density (IOD), which reflects the content of AQP3. When using t-test method for statistical analysis, compared with the BC group, the significance is represented by *, 0.01 < P-value < 0.05, and P-value < 0.01 is represented by **.

[0086] As can be seen from Table 8, hyaluronic acid lysine copper chelate, rhizoma polygonati extract, and radix stephaniae tetrandrae extract all have the effect of promoting the expression of AQP3. After the three are combined, the promotion of AQP3 has a 1+1+1>3 effect. Therefore, the skin care composition for improving redness and dryness problems can synergistically promote the expression of AQP3 content and has a repair effect.

[0087] Table 9 HAS1 gene analysis result summary table

[0088] As can be seen from Table 8, hyaluronic acid lysine copper chelate, rhizoma polygonati extract, and radix stephaniae tetrandrae extract all have the effect of promoting the expression of AQP3. After the three are combined, the promotion of AQP3 has a 1+1+1>3 effect. Therefore, the skin care composition for improving redness and dryness problems can synergistically promote the expression of AQP3 content and has a repair effect. Experimental group Mean SD P-value Boost rate BC 1.00 0.06 / / PC2 1.40 0.12 0.006** 40％ Example 1 1.23 0.09 0.018* 23％ Example 2 1.31 0.08 0.005** 31％ Example 3 1.46 0.1 0.002** 46％ Example 4 1.40 0.1 0.003** 40％ Comparative Example 1 1.13 0.04 0.0245* 13％ Comparative Example 2 1.01 0.05 0.819 / Comparative Example 3 1.09 0.04 0.072 9％ Comparative Example 4 1.14 0.03 0.014* 14％ Comparative Example 5 1.10 0.06 0.091 10％ Comparative Example 6 1.22 0.07 0.011* 22％

[0089] Note: 2 -△△CT The results were calculated by the method, and when the statistical analysis was performed by the t-test method, the mRNA amplification fold of the BC group was normalized, and compared with the BC group, the significance was indicated by *, P-value <0.05 was indicated by *, and P-value <0.01 was indicated by **.

[0090] As can be seen from Table 9, the powder of Stephania tetrandra extract does not have the effect of promoting the expression of HAS1, the hyaluronic acid lysine copper chelate and the extract of Polygonatum have the effect of promoting the expression of HAS1 gene, and the combination of the three has the effect of 1+1+1>3 on the promotion of HAS1 gene. Therefore, the skin care composition for improving the problems of redness and dryness can synergistically promote the expression of HAS1 gene and has the repair effect.

[0091] Test Example 3: In vitro activity test for inhibiting PGE2 and TNF-α factors

[0092] LPS (bacterial lipopolysaccharide) was used to induce mouse macrophage Raw264.7, and the expression amount of PGE2 and TNF-α was detected by ELISA method to evaluate the ability of the test substance to inhibit the expression of related cell pathway factors. See Table 10 for test scheme.

[0093] Table 10 Test scheme for in vitro activity test for inhibiting PGE2 and TNF-α factors

[0094]

[0095]

[0096] The test results are shown in Tables 11-12.

[0097] Table 11 Summary table of PGE2 content results

[0098] Sample name Mean concentration (pg / mL) SD P-value Inhibition rate BC 370.56 21.45 / / NC 39079.32 2073.53 0.000## / PC 26853.62 1453.89 0.001** 31.28％ Example 1 31689.42 1154.23 0.006** 18.91％ Example 2 28613.88 1425.78 0.002** 26.78％ Example 3 25233.52 1513.23 0.000** 35.43％ Example 4 27253.92 1456.65 0.001** 30.26％ Comparative Example 1 35214.38 856.11 0.041* 9.89％ Comparative Example 2 39112.65 1402.34 0.982 / Comparative Example 3 35382.42 731.12 0.044* 9.46％ Comparative Example 4 34112.34 878.91 0.019* 12.71％ Comparative Example 5 33924.76 1391.35 0.023* 13.19％ Comparative Example 6 30192.68 1389.24 0.004** 22.74％

[0099] Note: ## indicates that compared with the blank control, p<0.01; ** indicates that compared with the negative control, p<0.01; * indicates that compared with the negative control, 0.01

[0100] As can be seen from Table 11, the powder of Stephania tetrandra extract cannot inhibit the expression of PGE2, the hyaluronic acid lysine copper chelate and the extract of Polygonatum have a certain inhibitory effect on the expression of PGE2, and when the three are present, they can significantly inhibit the expression of PGE2, and the extract of Polygonatum can significantly synergistically promote the inhibitory effect of the hyaluronic acid lysine copper chelate and the powder of Stephania tetrandra extract on PGE2.

[0101] Table 12 Summary of TNF-α content results

[0102]

[0103]

[0104] Note: ## means p < 0.01 compared with blank control; ** means p < 0.01 compared with negative control; * means 0.01 < p < 0.05 compared with negative control.

[0105] As can be seen from Table 2, the hyaluronic acid lysine copper chelate compound cannot inhibit the expression of TNF-α, the Stephania tetrandra extract and the polygonatum extract have certain inhibitory effect on the expression of TNF-α, and when the three are present, the expression of TNF-α can be significantly inhibited. It can also be seen that the hyaluronic acid lysine copper chelate compound can significantly synergistically promote the inhibitory effect of the polygonatum extract and the Stephania tetrandra extract on TNF-α.

[0106] The above results show that the product containing the skin care composition for improving the redness and dryness of the skin can achieve the effect of soothing and moisturizing the skin, and can be used in skin care products to improve the redness and dryness of the skin.

[0107] It should be noted that the above examples are only used to illustrate the technical solutions of the present application but not to limit the present application. Although the present application is explained in detail with reference to the examples, the technical solutions of the present application can be modified or replaced by equivalents according to the needs of those skilled in the art without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A skin care composition for improving the problem of skin redness, dryness, characterized in that, The effective components are hyaluronic acid lysine copper chelate, Stephania tetrandra extract and polygonatum extract, and the mass fractions of the effective components are as follows: 0.1-1.0 parts of hyaluronic acid lysine copper chelate, 1.0-15.0 parts of Stephania tetrandra extract, and 1.0-15.0 parts of polygonatum extract; The preparation method of the Stephania tetrandra extract is as follows: the ultrasonic-assisted low-eutectic solvent extraction method is used for extraction, the extract is filtered, and glycerol is added after the filtrate is concentrated, wherein the concentration is 0.3-0.8 g / mL in terms of crude drug; The preparation method of the polygonatum extract is as follows: the supercritical fluid extraction method is used for extraction, the extract is filtered, and glycerol is added after the filtrate is concentrated, wherein the concentration is 0.4-1.0 g / mL in terms of crude drug.

2. The skin care composition for improving redness and dryness problems of the skin according to claim 1, wherein the composition further comprises a humectant. The composition further comprises the solvents butanediol and water.

3. The skincare composition for improving skin redness and dryness as described in claim 2, characterized in that, The components and mass fractions are as follows: 0.5-1.0 parts of hyaluronic acid lysine copper chelate, 5.0-10.0 parts of Stephania tetrandra extract, 5.0-10.0 parts of polygonatum extract, 12.0-18.0 parts of butanediol, and water to 100 parts.

4. The method of preparing a skin care composition according to claim 3, wherein the water-soluble polymer is added to the water phase. The polygonatum extract, the Stephania tetrandra extract and butanediol are mixed together, and then the hyaluronic acid lysine copper chelate is added and dissolved in purified water to 100 parts.

5. Use of the composition of claim 1 in the preparation of skin care cosmetics for improving the problems of skin redness and dryness.

6. The use according to claim 5, characterized in that, The composition promotes the expression of the moisture retention related factors HAS1 and AQP3, and inhibits the expression of the inflammation related factors PGE2 and TNF-α, so as to achieve the purpose of improving the problems of skin redness and dryness.

7. A skincare cosmetic that improves skin redness and dryness, characterized in that, The skin care composition for improving the problems of skin redness and dryness comprises the composition of claim 3.

8. The skin care cosmetic according to claim 7, wherein the skin care cosmetic is a skin care cosmetic for the treatment of skin aging. The composition is added in an amount of 1.0-10.0% based on the total mass of the skin care cosmetic. The composition is added in an amount of 1.0-10.0% based on the total mass of the skin care cosmetic.

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