Anti-hair loss composition, preparation method and use

Abstract

The present invention belongs to the field of hair care technology and discloses an anti-hair loss composition, preparation method, and use. The anti-hair loss composition comprises, by weight, a mixture of 70-99.9% of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, and 0.1-30% of Olea europaea leaf extract. The Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract are extracted from Platycladus orientalis leaf, Ganoderma lucidum, and Panax ginseng root, either individually or in combination, with the weight ratio of Platycladus orientalis leaf, Ganoderma lucidum, and Panax ginseng root being 1-9:0.1-3:0.1-3. The present invention synergistically enhances the anti-hair loss efficacy of the composition in terms of antibacterial properties, cell proliferation promotion, and antioxidant properties by combining the Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract, and Olea europaea leaf extract.

Description

Technical Field

[0001] The present invention relates to the technical field of hair care, and in particular to an anti-hair loss composition, a preparation method and application thereof. Background Art

[0002] Current research has found that postpartum hair loss is a common phenomenon in women, and it is a major problem faced by many new mothers. Postpartum hair loss is mainly caused by the following factors:

[0003] 1. Hair loss caused by changes in hormone levels: Women have higher estrogen levels during pregnancy and drop sharply after giving birth, causing hair follicles to enter the resting phase and causing hair loss;

[0004] 2. Care and washing of hair during the postpartum recovery period requires careful handling, resulting in a significant decrease in the frequency of hair care and washing. Furthermore, during the postpartum recovery period, the oil secretion of the scalp of women is unbalanced, leading to a decline in scalp health and hair loss.

[0005] 3. During the delivery process, mothers endure tremendous physical and psychological pressure, which may have a negative impact on the nervous system and further affect the blood circulation of the scalp and the hair growth environment.

[0006] Prior art 1: Chinese patent application 201911426717.1 discloses a medicine for hair or scalp and a preparation method thereof. The medicine for hair or scalp is prepared from the following components in the following weight ratios: 30-60 parts of Eclipta prostrata, 15-30 parts of Polygonum multiflorum, 25-50 parts of mulberry leaves, 10-35 parts of Platycladus orientalis leaves, 25-50 parts of Salvia miltiorrhiza, 10-55 parts of cedarwood alcohol, 2.5-5 parts of pumpkin seed oil, 1-5 parts of Artemisia annua, 3-8 parts of Sophora flavescens, 1-4 parts of cloves, 1.5-4 parts of Daphne genkwa, and 2-8 parts of Schizonepeta tenuifolia. The preparation method of the medicine for hair or scalp comprises the following steps: S1: preparing medicinal liquid a for standby use, S2: preparing medicinal liquid b for standby use, S3: preparing mixed powder c, and S4: preparing the medicine.

[0007] Existing technology 1 uses Chinese medicine such as Platycladus orientalis leaves to prepare medicine, which can kill head mites in depth and protect hair follicles, thereby achieving the effect of removing mites, controlling oil and promoting hair regeneration. It has the effects of growing and nourishing hair, strengthening hair and preventing hair loss, repairing scalp cells and hair follicles, replenishing pigments and controlling oil and removing dandruff simultaneously; and the Chinese medicine used in existing technology 1 is not very irritating, which provides certain ideas for women's postpartum anti-hair loss and anti-hair loss care.

[0008] However, the prior art 1 directly uses traditional Chinese medicine to make hair care products, which has the following defects:

[0009] (1) Chinese herbal medicine raw materials contain other impurities, some of which may even have side effects on hair or scalp;

[0010] (2) Since the cell walls of Chinese herbal medicines have not been completely destroyed, the efficiency of the hair care ingredients in them in directly acting on the hair or scalp is low, resulting in insufficient anti-hair loss effect.

[0011] Based on the above-mentioned defects, the problem of the prior art is that: no effective ingredients that directly act on the hair or scalp are effectively extracted to form an anti-hair loss composition, and the anti-hair loss effect is low. Summary of the Invention

[0012] One of the purposes of the present invention is to provide an anti-hair loss composition that is compounded with multiple plant extracts and can effectively act on the hair and scalp while synergistically improving the anti-hair loss efficacy.

[0013] Another object of the present invention is to provide a method for preparing an anti-hair loss composition, wherein the anti-hair loss composition prepared by this method can maximize the anti-hair loss efficacy of the composition.

[0014] Another object of the present invention is to provide a use of an anti-hair loss composition, which can be used to prepare daily chemical products such as hair care products, especially daily chemical products such as conditioners and shampoos, and can exert excellent anti-hair loss effects.

[0015] To achieve the above object, the present invention provides an anti-hair loss composition, comprising, by weight percentage, a mixture of 70-99.9% of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, and 0.1-30% of Olea europaea leaf extract;

[0016] The Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract are obtained by extracting Platycladus orientalis leaf, Ganoderma lucidum and ginseng root separately or in combination. The weight ratio of Platycladus orientalis leaf, Ganoderma lucidum and ginseng root is 1-9:0.1-3:0.1-3.

[0017] The volatile oil contained in Platycladus orientalis leaf extract can prevent the spread of Staphylococcus aureus, remove dandruff and promote hair growth, and has a certain protective effect on hair follicle damage caused by cyclophosphamide, slowing down the hair from entering the regression phase, thereby playing a role in preventing and treating hair loss; and the total flavonoids in Platycladus orientalis leaf extract can activate the stem cell characteristics of hair matrix cells to divide, proliferate, and differentiate, thereby restoring vitality to some hair follicles.

[0018] Ganoderma lucidum, also known as Lingzhi, is a nourishing and strengthening herb that strengthens the body and strengthens the body. It has the effects of tonifying Qi, calming the mind, relieving coughs and asthma, and has a certain auxiliary therapeutic effect on postpartum hair loss. It has the effects of nourishing Qi and blood, promoting blood circulation and removing blood stasis, and strengthening the stomach and calming the mind. In his "Golden Chamber", Zhang Zhongjing believed that hair is related to the heart, lungs, liver, kidneys, essence, Qi, and blood. The heart governs blood vessels and consciousness; the lungs govern hair growth and are responsible for the skin and fur; the liver stores blood and regulates qi and blood flow; and the kidneys store essence, the essence of which is manifested in hair. From a Traditional Chinese Medicine perspective, postpartum hair loss may be caused by insufficient kidney essence and qi and blood deficiency. Lingzhi, with its kidney-tonifying, qi-invigorating, and blood-nourishing properties, has a certain effect on postpartum hair loss caused by insufficient kidney essence and qi and blood deficiency. Modern pharmacological research shows that Lingzhi can regulate the body's immune function, enhance physical fitness, dilate blood vessels, promote blood circulation, lower blood lipids, and provide a calming effect.

[0019] Ginseng is a top-grade tonic and health-care product, known as the "King of Herbs". The main active ingredient of ginseng is ginsenoside, and its main medicinal value is to promote the differentiation of hair follicles, activate blood circulation, and have anti-inflammatory and antibacterial properties. The Compendium of Materia Medica records that consuming ginseng after childbirth can replenish deficiency and activate blood circulation. Ginseng can expand the capillaries in the head and improve the nutritional status of the hair; the effective ingredients of ginseng are absorbed by the hair, which can increase the tensile strength and extensibility of the hair, making the hair smoother. Ginseng has the function of preventing and reducing hair loss, promoting hair growth and development, and the effect is significant; the mechanism of ginseng in hair growth and preventing hair loss is:

[0020] 1) Ginseng contains plant hormones related to estrogen. The presence of these substances can improve blood circulation in the head skin and regulate immunity, thereby promoting hair growth.

[0021] 2) Reduce the apoptosis and degeneration of keratinocytes in the growth phase of hair follicles.

[0022] Ginseng root extract can dilate the capillaries in the head, increase the nutritional supply of the hair, and improve the toughness of the hair, thereby reducing hair loss and hair breakage, and also has a protective effect on damaged hair; Ginseng root extract contains ginsenosides and polysaccharides, ginsenosides being its main component, which is relatively easy to penetrate the surface of the skin and be absorbed by the dermis, and has a protective function for the skin and hair; Ginseng extract can also inhibit DKK-1-induced hair follicle degeneration by regulating the expression of Bcl-2 and Bax, thereby stimulating the proliferation of outer root sheath keratinocytes and reducing hair loss.

[0023] Olive leaf extract contains a variety of anti-inflammatory and antioxidant ingredients, such as oleuropein, hydroxytyrosol, etc., which can effectively avoid the oxidative stress response of the scalp and effectively slow down hair loss.

[0024] Preferably, the anti-hair loss composition further comprises 0.001-2% 10-hydroxydecanoic acid; 10-hydroxydecanoic acid can effectively inhibit 5α-reductase.

[0025] Preferably, the anti-hair loss composition further comprises 0.1-10% capryloyl glycine; capryloyl glycine can effectively regulate the pH environment of sebum and effectively avoid the problem of hair damage caused by untimely hair care.

[0026] Preferably, the anti-hair loss composition further comprises 0.1 to 40% polyglyceryl-10 laurate; polyglyceryl-10 laurate has a strong cleansing effect and can effectively clean impurities such as debris on the hair and scalp.

[0027] The present invention also provides a method for preparing the above-mentioned anti-hair loss composition, which comprises mixing Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract to obtain the anti-hair loss composition.

[0028] In the technical solution of the present invention, the Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract, and Olea europaea leaf extract can all be obtained through commercially available products.

[0029] Further preferably, the mixture of the Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract is obtained by composite extraction of Platycladus orientalis leaf, Ganoderma lucidum and ginseng root.

[0030] Furthermore, the specific steps of the composite extraction include:

[0031] Step 1: adding Platycladus orientalis leaves to water and performing ultrasonic extraction to obtain a mixture;

[0032] Step 2: Adding red ganoderma lucidum, ginseng root and water to the mixture for composite extraction, and then separating to obtain a filtrate;

[0033] Step 3: adding the filtrate to water for ultrasonic treatment to obtain a treated solution;

[0034] Step 4: subjecting the treated liquid to ethanol precipitation for 12 to 14 hours to obtain an alcohol precipitation solution, which is then concentrated to obtain an alcohol-free concentrate;

[0035] Step 5: adding an extractant to the alcohol-free concentrate to extract to obtain an extract;

[0036] Step 6: ultrafiltration of the extract to obtain the permeate to obtain Platycladus orientalis leaf extract, Ganoderma lucidum extract and Panax ginseng root extract;

[0037] In the step 1, the ultrasonic extraction temperature is 55-65° C., the ultrasonic power is 180 W, the ultrasonic time is 40-45 min, the weight ratio of Platycladus orientalis leaves to water is 1:1-3; the weight ratio of Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots is 1-9:0.1-3:0.1-3.

[0038] Preferably, the specific operation of step 2 is: adding red ganoderma lucidum, ginseng root and water to the mixture, extracting at 45-55° C. for 2 hours and separating to obtain a first filtrate and a filter residue, then adding water to the filter residue, extracting at 45-55° C. for 2 hours and separating to obtain a second filtrate, and mixing the first filtrate and the second filtrate to obtain a mixed filtrate;

[0039] In the step 2, the amount of water added in each extraction is 4 to 8 times the total weight of the Platycladus orientalis leaves, Ganoderma lucidum and ginseng roots.

[0040] In step 3, the weight ratio of the filtrate to water is 10:1, the ultrasonic power of the ultrasonic treatment is 180 W, and the ultrasonic time is 20 to 25 minutes.

[0041] Preferably, the specific operation of step 4 is: first using a single-effect concentrator to concentrate the treated liquid to 10%-20% of the original mass, then adding an equal mass of 95% ethanol solution and standing for 12-14 hours, centrifuging, taking the supernatant to obtain an alcohol precipitate, transferring the alcohol precipitate to a falling film concentrator, and vacuum concentrating the filtrate to zero ethanol content at a concentration temperature of 50-60° C. and a concentration pressure of -0.07 MPa to -0.09 MPa to obtain an alcohol-free concentrate.

[0042] Preferably, the extractant in step 5 is at least one of water, butylene glycol, glycerin, and methylpropylene glycol, the amount of the extractant added is 1 to 5 times the total weight of Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots, and the extraction time is 30 to 50 minutes.

[0043] Preferably, the specific operation of step 6 is: adding the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 150kDa to 500kDa for ultrafiltration to obtain a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

[0044] The present invention also discloses a use of the anti-hair loss composition for preparing daily-use cleaning and care products.

[0045] Beneficial effects

[0046] Compared with the prior art, the present invention has at least the following advantages:

[0047] (1) The present invention improves the anti-hair loss efficacy of the composition by compounding Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract in terms of antibacterial, cell proliferation promotion and anti-oxidation.

[0048] (2) The present invention effectively improves the synergistic anti-hair loss efficacy of the composition by using the technical means of composite extraction of Platycladus orientalis leaves and olive leaves;

[0049] (3) The anti-hair loss composition of the present invention can effectively prevent hair loss and the shrinkage and closure of scalp hair follicles when used in daily chemical products. In particular, it can provide efficient hair care for women during the postpartum recovery period and avoid problems such as hair damage and hair loss caused by not washing hair for a long time during the recovery period. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0051] Figure 1 This is a diagram of a chicken embryo before using Application Example 1 of the present invention;

[0052] Figure 2 This is a picture of a chicken embryo after using Application Example 1 of the present invention. DETAILED DESCRIPTION

[0053] The present invention will be further described below in conjunction with the embodiments, but this does not constitute any limitation to the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0054] In order to explain the technical content of the present invention in detail, further description will be given below in conjunction with the embodiments.

[0055] In the following examples and comparative examples, the olive leaf extract was purchased from Shanghai Boshuo Industrial Co., Ltd., and the ginger root extract, Polygonum multiflorum extract, Angelica sinensis root extract, and Centella asiatica extract were purchased from Guangzhou Hemiao Biotechnology Co., Ltd.

[0056] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0057] In the following examples and comparative examples, unless otherwise specified, % and parts are percentages by weight and parts by weight, respectively.

[0058] Example 1

[0059] An anti-hair loss composition is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract with 1.79% of Olea europaea leaf extract;

[0060] The Platycladus orientalis leaf extract, Ganoderma lucidum extract and Panax ginseng root extract are respectively extracted from 20 parts of Platycladus orientalis leaves, 1 part of Ganoderma lucidum and 1 part of Panax ginseng root according to the following steps:

[0061] Step 1: crush the raw materials and sieve to obtain 100-mesh particles;

[0062] Step 2: adding 3 times the weight of water to the particles obtained in step 1, extracting at 50° C. for 2 hours, and separating to obtain a first filtrate and a filter residue; then adding water to the filter residue, extracting at 50° C. for 2 hours, and separating to obtain a second filtrate; and mixing the first filtrate and the second filtrate to obtain a water-soluble extract of Platycladus orientalis leaves, Ganoderma lucidum, or Panax ginseng roots;

[0063] Step 3: The water-soluble extract was subjected to ethanol precipitation for 12 hours to obtain an alcohol extract, and the alcohol extract was transferred to a falling film concentrator. The filtrate was vacuum concentrated at a concentration temperature of 55° C. and a concentration pressure of -0.08 MPa to zero ethanol content to obtain an alcohol-free concentrate;

[0064] Step 4: adding an extractant to the alcohol-free concentrate and performing extraction for 30 minutes to obtain an extract;

[0065] Step 5: adding the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 300 kDa to perform ultrafiltration to extract the permeate to obtain Platycladus orientalis leaf extract, Ganoderma lucidum extract or Panax ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

[0066] Example 2

[0067] An anti-hair loss composition is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract with 1.79% of Olea europaea leaf extract;

[0068] The mixture of the Platycladus orientalis leaf extract, Ganoderma lucidum extract and ginseng root extract is obtained by composite extraction of Platycladus orientalis leaf, Ganoderma lucidum and ginseng root. The weight ratio of Platycladus orientalis leaf, Ganoderma lucidum and ginseng root is 20:1:1. The specific steps of the composite extraction include:

[0069] Step 1: Crush the Platycladus orientalis leaves, Ganoderma lucidum and Ginseng root separately and sieve them into 100 mesh Platycladus orientalis leaves, Ganoderma lucidum and Ginseng root particles.

[0070] Step 2: adding the Platycladus orientalis leaf particles to water 3 times the weight of the Platycladus orientalis leaf particles, and performing ultrasonic extraction at an ultrasonic extraction temperature of 60° C. and an ultrasonic power of 180 W for 40 minutes to obtain a mixture;

[0071] Step 3: adding Ganoderma lucidum granules, Ganoderma lucidum root granules and 5 times the total weight of Platycladus orientalis leaf granules, Ganoderma lucidum granules, and Ginseng root granules to the mixture, extracting at 45-55° C. for 2 hours and separating to obtain a first filtrate and a filter residue, then adding 5 times the total weight of Platycladus orientalis leaf granules, Ganoderma lucidum granules, and Ginseng root granules to the filter residue, extracting at 45-55° C. for 2 hours and separating to obtain a second filtrate, and mixing the first filtrate and the second filtrate to obtain a mixed filtrate;

[0072] Step 4: adding the filtrate to water for ultrasonic treatment at room temperature to obtain a treated liquid, wherein the weight ratio of the filtrate to water is 10:1, the ultrasonic power of the ultrasonic treatment is 180 W, and the ultrasonic time is 20 min;

[0073] Step 5: First, the treated liquid is concentrated to 15% of the original mass using a single-effect concentrator, and then an equal mass of 95% ethanol solution is added and allowed to stand for 12 hours. The mixture is centrifuged and the supernatant is collected to obtain an alcohol precipitate. The alcohol precipitate is transferred to a falling film concentrator, and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 60°C and a concentration pressure of -0.08 MPa to obtain an alcohol-free concentrate;

[0074] Step 6: adding water to the alcohol-free concentrate for extraction to obtain an extract, wherein the amount of water is 3 times the total weight of the Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots, and the extraction time is 30 minutes;

[0075] Step 7: adding the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 300 kDa for ultrafiltration to obtain the composite extracted Platycladus orientalis leaf extract, Ganoderma lucidum extract and Panax ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

[0076] Example 3

[0077] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 96.5% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract with 3.5% of Olea europaea leaf extract.

[0078] Example 4

[0079] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 97.2% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 1.8% of Olea europaea leaf extract, and 1% of 10-hydroxydecanoic acid.

[0080] Example 5

[0081] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 88.4% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 1.6% of Olea europaea leaf extract, and 10% of capryloylglycine.

[0082] Example 6

[0083] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 88.4% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 1.6% of Olea europaea leaf extract, and 10% of polyglycerol-10 laurate.

[0084] Example 7

[0085] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 62% of a mixture of arborvitae leaf extract, red ganoderma extract, and ginseng root extract, 3% of olive leaf extract, 0.08% of 10-hydroxydecanoic acid, 6% capryloyl glycine, and 28.92% of polyglycerol-10 laurate.

[0086] Comparative Example 1

[0087] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 83.4% of a mixture of Ganoderma lucidum extract and ginseng root extract and 16.6% of olive leaf extract; wherein the mixture of Ganoderma lucidum extract and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of Ganoderma lucidum to ginseng root is 1:1.

[0088] Comparative Example 2

[0089] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing a mixture of 98.1% of Platycladus orientalis leaf extract and ginseng root extract and 1.9% of olive leaf extract; wherein the mixture of Platycladus orientalis leaf extract and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of the Platycladus orientalis leaf and ginseng root is 20:1.

[0090] Comparative Example 3

[0091] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.1% of a mixture of arborvitae leaf extract and ganoderma lucidum extract and 1.9% of olive leaf extract; wherein the mixture of arborvitae leaf extract and ganoderma lucidum root extract is extracted by the composite extraction method of Example 2, and the weight ratio of the arborvitae leaf and ganoderma lucidum is 20:1.

[0092] Comparative Example 4

[0093] The method is substantially the same as Example 2, except that the anti-hair loss composition is 100% Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract.

[0094] Comparative Example 5

[0095] It is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.21% of a mixture of ginger root extract, red ganoderma lucidum extract, and ginseng root extract and 1.79% of olive leaf extract; wherein the mixture of ginger root extract, red ganoderma lucidum root extract, and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of the ginger root, red ganoderma lucidum, and ginseng root is 20:1:1.

[0096] Comparative Example 6

[0097] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.21% of a mixture of arborvitae leaf extract, polygonum multiflorum extract, and ginseng root extract and 1.79% of olive leaf extract; wherein the mixture of arborvitae leaf extract, polygonum multiflorum extract, and ginseng root extract is extracted by the composite extraction method of Example 2, and the weight ratio of the arborvitae leaf, polygonum multiflorum, and ginseng root is 20:1:1.

[0098] Comparative Example 7

[0099] It is generally the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.21% of a mixture of arborvitae leaf extract, red ganoderma extract, and angelica extract and 1.79% of olive leaf extract; wherein the mixture of arborvitae leaf extract, red ganoderma extract, and angelica extract is extracted by the composite extraction method of Example 2, and the weight ratio of the arborvitae leaf, red ganoderma, and angelica is 20:1:1.

[0100] Comparative Example 8

[0101] The method is substantially the same as Example 2, except that the anti-hair loss composition is obtained by mixing 98.21% of a mixture of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract and 1.79% of Centella asiatica extract.

[0102] Performance Testing

[0103] 5α-reductase inhibition rate

[0104] 1. Test purpose and principle

[0105] Oily skin has a highly active sebaceous gland secretion function, resulting in a greasy feeling on the face, difficulty in cleaning, and an unsightly sheen. It is also prone to skin diseases such as acne vulgaris and seborrheic dermatitis. Given the relationship between 5α-reductase and sebaceous glands, the inhibitory effect of test samples on 5α-reductase is often used to reflect the sebum regulation effect and thus characterize the oil control efficacy of the test sample.

[0106] This test refers to the laboratory method (HMC-WI-031 5α-reductase inhibition rate) and compares the 5α-reductase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and the difference is significant (P<0.05), it can be considered that the test sample has a certain oil control effect.

[0107] This test method is an in vitro method and is suitable for evaluating cosmetic products that claim to achieve oil control by inhibiting 5α-reductase.

[0108] 2 Test indicators

[0109] Table 1 Test indicators

[0110]

[0111] 3 Experimental materials and methods

[0112] 3.1 Instruments and Equipment

[0113] BSA224S Analytical Balance

[0114] Agilent 1220 HPLC

[0115] 3.2 Reagents

[0116] Testosterone, 98%

[0117] NADPH, 90%

[0118] Anhydrous ethanol

[0119] 3.3 Test methods

[0120] (1) Treatment of control and test samples

[0121] Sample group: Examples 1-7 and Comparative Examples 1-8 were diluted with pure water to a sample concentration of 10%;

[0122] Positive control (finasteride, purity ≥98%): Dilute with anhydrous ethanol to a positive control concentration of 0.1%;

[0123] Negative control: PBS solution.

[0124] (2) Test operation steps

[0125] Set up a sample group, a positive control group, a negative control group and a blank group. Each group needs to set up 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and perform HPLC analysis on each group to determine the testosterone content in each tube.

[0126] (3) Calculation formula

[0127]

[0128] (4) Data Analysis

[0129] SPSS was used for statistical analysis, and the independent sample t-test was used to compare the 5α-reductase inhibition rates among the test samples, positive controls, and negative controls.

[0130] The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 indicates no significant difference between the two groups; P<0.05 indicates a significant difference between the two groups.

[0131] The anti-shedding compositions prepared in Examples 1-7 and Comparative Examples 1-8 were tested according to the above test method. The results are shown in Table 2.

[0132] Table 2 5α-reductase inhibition rate of the anti-hair loss compositions prepared in Examples 1-7 and Comparative Examples 1-8

[0133]

[0134]

[0135] According to the results in Table 2, we can see that:

[0136] According to the data of Examples 1-7, the present invention uses a combination of Platycladus orientalis leaf extract, Ganoderma lucidum extract, Panax ginseng root extract and Olea europaea leaf extract to exert a significant 5α-reductase inhibitory effect;

[0137] A comparison of the data in Example 1 and Example 2 shows that there is a significant difference in the 5α-reductase inhibition rate that can be exerted by the separate extraction and the combined extraction of Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots. It is preliminarily speculated that this is because the effective ingredients in the combined extraction of Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots have a synergistic solubilization effect, thereby increasing the content of the effective ingredients in the entire combined extract and effectively improving the 5α-reductase inhibition efficacy of the anti-hair loss composition.

[0138] According to the data comparison of Examples 4-6 and Example 2, since 10-hydroxydecanoic acid, capryloyl glycine, and polyglycerol-10 laurate have different effects, the 5α-reductase inhibition rates of Examples 4-6 are also different under the same total dosage. The addition of 10-hydroxydecanoic acid in Example 4 improves the 5α-reductase inhibition rate of the composition to a certain extent. In Examples 5 and 6, the inhibition effect of the composition on 5α-reductase is reduced because the dosage of other components is reduced and capryloyl glycine and polyglycerol-10 laurate do not have a high inhibitory effect on 5α-reductase. However, in Example 7, 10-hydroxydecanoic acid and capryloyl glycine are used to inhibit the 5α-reductase of the composition. The technical solution of adding a combination of 10-hydroxydecanoic acid, capryloyl glycine and polyglyceryl-10 laurate to Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract has a significantly improved 5α-reductase inhibitory effect compared to Example 2. It is speculated that the combination of 10-hydroxydecanoic acid, capryloyl glycine and polyglyceryl-10 laurate can synergistically enhance the 5α-reductase inhibitory effect of the combined composition of Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract. It may be that the combination of 10-hydroxydecanoic acid, capryloyl glycine and polyglyceryl-10 laurate comprehensively protects and cleanses the scalp, thereby exerting a stronger 5α-reductase inhibitory effect of the composition.

[0139] According to the data comparison of Example 2 and Comparative Examples 1-4, it can be seen that in the absence of any one of the Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract, the 5α-reductase inhibition rate of the composition is significantly reduced, indicating that the synergistic effect of the composition of the present invention in enhancing the 5α-reductase inhibition efficacy is based on the combination of Platycladus orientalis leaf extract, Ganoderma lucidum extract, ginseng root extract and olive leaf extract, and none of them can be missing.

[0140] According to the data comparison of Example 2 and Comparative Examples 5-8, although the technical solution of using ginger root extract to replace Platycladus orientalis leaf extract, Polygonum multiflorum extract to replace Ganoderma lucidum extract, Angelica sinensis root extract to replace Ginseng root extract, and Centella asiatica extract to replace Olive leaf extract can also produce good 5α-reductase inhibition effect, it is significantly lower than that of Example 2, indicating that the composition of the present invention is a synergistic combination of Platycladus orientalis leaf extract, Ganoderma lucidum extract, Ginseng root extract, and Olive leaf extract.

[0141] Application Example 1

[0142] An anti-hair loss essence, the formula ratio is shown in Table 3.

[0143] Table 3 Formula of anti-hair loss essence

[0144] Raw material name Addition amount wt / % The anti-shedding composition of Example 2 2.24 10-Hydroxydecanoic acid 0.045 Capryloylglycine 0.11 Polyglyceryl-10 Laurate 0.101 Disodium EDTA 0.05 Dipotassium glycyrrhizate 0.15 Butanediol 5 Betaine 3 Sodium polyacryloyldimethyl taurate 0.5 p-Hydroxyacetophenone 0.4 1,2-Hexanediol 0.4 water margin

[0145] Application Example 2

[0146] An anti-hair loss shampoo, the formula ratio is shown in Table 4.

[0147] Table 4: Formula of Anti-Hair Loss Shampoo

[0148]

[0149]

[0150] Application Example 3

[0151] An anti-hair loss shampoo, the formula ratio is shown in Table 5.

[0152] Table 5: Formula of Anti-Hair Loss Shampoo

[0153]

[0154]

[0155] Security Testing

[0156] Test method: Chicken embryo chorioallantoic membrane test

[0157] 1. Test purpose and principle

[0158] The chick chorioallantoic membrane test is an early in vitro method for evaluating eye irritation. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chick embryo. This test utilizes the intact, clear, and transparent vascular system of the chorioallantoic membrane in mid-stage incubation. A certain amount of the test substance is directly exposed to the chorioallantoic membrane. After a period of exposure, changes in toxic effect indicators (such as bleeding, coagulation, and vascular melting) are observed. These indicators reflect changes in the morphology, structure, color, and permeability of the blood vessels and vascular network, as well as phenomena such as chorioallantoic membrane protein denaturation and the degree of damage. These indicators are then combined to generate a score used to assess the eye irritation potential of the test substance.

[0159] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the factors and processes of the potential eye irritation of the evaluated substance.

[0160] 2. Experimental Group Treatment and Reagents

[0161] Sample group: Application Examples 1-3 were diluted into 2% aqueous solution respectively.

[0162] Negative control: 0.9% saline.

[0163] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0164] 3. Experimental steps

[0165] Six chick embryos were used in each group for this test. The chorioallantoic membrane (CAM) was recorded using a camera. A polytetrafluoroethylene (PTFE) ring was placed on the CAM and photographed. The sample to be tested was added to the ring, the time of sample addition was recorded, and the air chamber was covered with moistened plastic wrap. The embryos were then transferred to a constant temperature and humidity chamber for incubation, and the extent of each toxic effect was observed.

[0166] 4. Calculation of results

[0167] The endpoint evaluation method was used to calculate the endpoint evaluation (ES) with the result rounded to two decimal places. Each chicken embryo was scored as the sum of the bleeding, coagulation and vascular lysis observed in each chicken embryo. The average value of the mathematical sum obtained for ES-6 chicken embryos was obtained.

[0168] The average ES score was calculated using the following formula.

[0169]

[0170] 5. Result judgment criteria

[0171] ES≤4, non-irritating;

[0172] 4<ES≤12, mild irritation;

[0173] 12<ES<16, moderate irritation;

[0174] ES ≥ 16, strong irritant / corrosive.

[0175] 2. Test Results

[0176] According to the above test method, application examples 1-3 were tested and the ES values ​​were all less than 4, indicating that they were non-irritating.

[0177] Among them, the results of chicken embryos before and after use in Application Example 1 are as follows Figure 1-Figure 2 As shown, the sample test result of Application Example 1 is ES=2.33;

[0178] This proves that the technical solution of the present invention is mild and non-irritating.

[0179] The embodiments presented herein are merely embodiments selected from a combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include subranges therein, and variations in these ranges should also be covered by the appended claims.

Claims

1. An anti-shedding composition, characterized in that By weight percentage, it is composed of a mixture of 62-99.9% of Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract, 0.1-30% of Olea europaea leaf extract, 0.001-2% of 10-hydroxydecanoic acid, 0.1-10% of capryloyl glycine, and 0.1-40% of polyglyceryl-10 laurate; The Platycladus orientalis leaf extract, Ganoderma lucidum extract, and Panax ginseng root extract are obtained by composite extraction of Platycladus orientalis leaf, Ganoderma lucidum, and Panax ginseng root, wherein the weight ratio of Platycladus orientalis leaf, Ganoderma lucidum, and Panax ginseng root is 1-9:0.1-3:0.1-3; The composite extraction method of the Platycladus orientalis leaf extract, Ganoderma lucidum extract and Ginseng root extract is as follows: Step 1: Crush and sieve the Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots into 100-mesh granules; Step 2: adding the Platycladus orientalis leaf particles to water 3 times the weight of the Platycladus orientalis leaf particles, and performing ultrasonic extraction at an ultrasonic extraction temperature of 60° C. and an ultrasonic power of 180 W for 40 minutes to obtain a mixture; Step 3: adding Ganoderma lucidum granules, Ginseng root granules, and 5 times the total weight of Platycladus orientalis leaf granules, Ganoderma lucidum granules, and Ginseng root granules to the mixture, extracting at 45-55° C. for 2 hours and separating to obtain a first filtrate and a filter residue, then adding 5 times the total weight of Platycladus orientalis leaf granules, Ganoderma lucidum granules, and Ginseng root granules to the filter residue, extracting at 45-55° C. for 2 hours and separating to obtain a second filtrate, and mixing the first filtrate and the second filtrate to obtain a mixed filtrate; Step 4: adding the filtrate to water for ultrasonic treatment at room temperature to obtain a treated liquid, wherein the weight ratio of the filtrate to water is 10:1, the ultrasonic power of the ultrasonic treatment is 180 W, and the ultrasonic time is 20 min; Step 5: First, the treated liquid is concentrated to 15% of the original mass using a single-effect concentrator, and then an equal mass of 95% ethanol solution is added and allowed to stand for 12 hours. The mixture is centrifuged and the supernatant is collected to obtain an alcohol precipitate. The alcohol precipitate is transferred to a falling film concentrator and the filtrate is vacuum concentrated to zero ethanol content at a concentration temperature of 60°C and a concentration pressure of -0.08 MPa to obtain an alcohol-free concentrate. Step 6: adding water to the alcohol-free concentrate for extraction to obtain an extract, wherein the amount of water is 3 times the total weight of the Platycladus orientalis leaves, Ganoderma lucidum, and ginseng roots, and the extraction time is 30 minutes; Step 7: adding the extract to an ultrafiltration device loaded with an ultrafiltration membrane with a molecular weight of 300 kDa for ultrafiltration to obtain the composite extracted Platycladus orientalis leaf extract, Ganoderma lucidum extract and Panax ginseng root extract, wherein the temperature during ultrafiltration is controlled within 35°C.

2. A use of the anti-hair loss composition according to claim 1 in preparing daily-use cleaning products.

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