Nano decomposition agent and method for accelerating decomposition of chicken manure

Abstract

The invention discloses a nano decomposition agent and a method for accelerating decomposition of chicken manure, and relates to the technical field of breeding industry. The nano decomposition agent disclosed by the invention is prepared from the following raw material components in parts by mass: 3 to 4 parts of compound bacteria, 6 to 8 parts of folium lycii extract, 0.3 to 0.5 part of nano iron oxide and 0.5 to 0.8 part of 2-hydroxyethyl picric acid, the compound bacteria are a mixture of bacillus amyloliquefaciens and thiamine bacillus. The nano decomposition agent disclosed by the invention can be used for quickly fermenting chicken manure materials, remarkably reducing the nitrogen content in chicken manure in a short time, increasing the carbon-nitrogen ratio and efficiently removing volatile organic compounds in the chicken manure. The preparation method of the nano-decomposition agent is simple, and the nano-decomposition agent has a wide application prospect in the aspect of chicken manure decomposition treatment.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a nano-composting agent and a method for accelerating the composting of chicken manure. Background Art

[0002] Poultry and livestock manure is an important organic waste. With the rapid development of the breeding industry, the output of poultry and livestock manure has shown an increasing trend. However, most of the poultry and livestock manure cannot be fully utilized and is piled up for a long time. The volatile odor components it contains or the volatile odor components released during the stacking process, such as organic amines, sulfur-containing substances, ketones, etc., have serious environmental hazards and may also cause poultry diseases. There are important safety hazards to human health. It is extremely necessary to properly handle them.

[0003] Chicken manure contains a large amount of nutrients, with high nitrogen content, low carbon-nitrogen ratio, and high VOC content. In addition, the composting process takes 30-60 days, which is a significant problem of long processing cycle. How to significantly shorten the composting cycle, improve the carbon-nitrogen ratio and reduce the VOC content on the basis of rapid composting of chicken manure is a technical problem that needs to be solved for chicken manure composting. Summary of the invention

[0004] The purpose of the present invention is to provide a nano composting agent and a method for accelerating the composting of chicken manure to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] One of the purposes of the present invention is to provide a nano-composting agent, the raw materials of which include the following components in parts by weight:

[0007] 3-4 parts of compound bacteria, 6-8 parts of wolfberry leaf extract, 0.3-0.5 parts of nano iron oxide and 0.5-0.8 parts of 2-hydroxyethyl picric acid.

[0008] The composite bacteria is a mixture of Bacillus amyloliquefaciens and Bacillus thiamine-lyticus.

[0009] Furthermore, the mass ratio of the Bacillus amyloliquefaciens to the Bacillus thiaminesolyticus is 1:1-2.

[0010] Furthermore, the deposit number of the Bacillus amyloliquefaciens is CGMCC No.17841; the deposit number of the Bacillus thiaminesolyticus is CCTCCM2020604.

[0011] Furthermore, the preparation method of the wolfberry leaf extract comprises the following steps:

[0012] The wolfberry leaves are added to ethanol for reflux extraction to obtain an extract, the extract is extracted with petroleum ether, the petroleum ether phase is collected, concentrated and dried to obtain the wolfberry leaf extract.

[0013] Furthermore, the volume concentration of the ethanol is 80%-90%.

[0014] The second object of the present invention is to provide a method for preparing the above-mentioned nano-composting agent, comprising the following steps:

[0015] The wolfberry leaf extract, the composite bacteria, the nano iron oxide and the 2-hydroxyethyl picric acid are mixed according to a mass ratio to obtain a nano composting agent.

[0016] The third object of the present invention is to provide the application of the above-mentioned nano-composting agent in the composting of chicken manure.

[0017] The fourth object of the present invention is to provide a method for accelerating the decomposition of chicken manure, comprising the following steps:

[0018] The above-mentioned nano-composting agent is mixed with fresh chicken manure and composted for 8-10 days to achieve rapid decomposition of the chicken manure.

[0019] Furthermore, the amount of the nano-composting agent added to fresh chicken manure is 0.8%-1.2%.

[0020] The present invention adopts a composite fermentation bacteria composed of Bacillus amyloliquefaciens and Bacillus thiamine-solubilizing, and compounded with wolfberry leaf extract, nano iron oxide and 2-hydroxyethyl picric acid components as a decomposition agent for rapid decomposition of chicken manure, and can maintain a long high-temperature fermentation time during the decomposition process. The strain compounding of the present invention is reasonable, the added nano iron oxide and 2-hydroxyethyl picric acid can promote microbial mutualism and ensure the activity of microorganisms, and the wolfberry leaf extract can also provide the composite bacteria with nutrients required for the fermentation process, so as to achieve efficient and rapid decomposition of chicken manure and significantly shorten the fermentation and decomposition cycle.

[0021] The present invention discloses the following technical effects:

[0022] The nanometer composting agent of the present invention can quickly ferment chicken manure materials, significantly reduce the nitrogen content in the chicken manure in a short time, improve the carbon-nitrogen ratio, and efficiently remove volatile organic matter in the chicken manure.

[0023] The nanometer composting agent of the invention has a simple preparation method and has broad application prospects in chicken manure composting treatment. DETAILED DESCRIPTION

[0024] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0025] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0026] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0027] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0028] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0029] In the following examples of the present invention, the Bacillus amyloliquefaciens used was purchased from the General Microbiological Center of China Microorganism Culture Collection Administration, with a collection number of CGMCC No.17841; the Bacillus thiamine-solubilizing used was purchased from the China Center for Type Culture Collection, with a collection number of CCTCCM2020604.

[0030] Example 1

[0031] A nano composting agent, the raw material mass proportion is as follows:

[0032] 4 parts of composite bacteria, 7 parts of wolfberry leaf extract, 0.5 parts of nano iron oxide and 0.6 parts of 2-hydroxyethyl picric acid.

[0033] The composite bacteria is a mixture of Bacillus amyloliquefaciens and Bacillus thiaminesolyticus in a mass ratio of 1:1.5.

[0034] The preparation steps of wolfberry leaf extract are as follows:

[0035] Wash the wolfberry leaves, add them to 85% v / v ethanol solution, reflux and extract for 3 times, each time for 0.5 hour, combine the extracts, filter, cool, then add petroleum ether and extract for 3 times, collect the petroleum ether phase, concentrate and dry to obtain the wolfberry leaf extract.

[0036] Preparation of nano composting agent:

[0037] The wolfberry leaf extract, compound bacteria, nano iron oxide and 2-hydroxyethyl picric acid are mixed according to a certain proportion to obtain a nano composting agent.

[0038] Example 2

[0039] A nano composting agent, the raw material mass proportion is as follows:

[0040] 3 parts of composite bacteria, 8 parts of wolfberry leaf extract, 0.4 parts of nano iron oxide and 0.8 parts of 2-hydroxyethyl picric acid.

[0041] The composite bacteria is a mixture of Bacillus amyloliquefaciens and Bacillus thiaminesolyticus in a mass ratio of 1:2.

[0042] The preparation steps of wolfberry leaf extract are as follows:

[0043] Take wolfberry leaves, add them to 85% v / v ethanol solution, reflux extract for 3 times, each time for 0.5 hour, combine the extracts, filter, cool, then add petroleum ether for extraction 3 times, collect the petroleum ether phase, concentrate and dry to obtain wolfberry leaf extract.

[0044] Preparation of nano composting agent:

[0045] The wolfberry leaf extract, compound bacteria, nano iron oxide and 2-hydroxyethyl picric acid are mixed according to a certain proportion to obtain a nano composting agent.

[0046] Example 3

[0047] A nano composting agent, the raw material mass proportion is as follows:

[0048] 4 parts of composite bacteria, 6 parts of wolfberry leaf extract, 0.3 parts of nano iron oxide and 0.5 parts of 2-hydroxyethyl picric acid.

[0049] The composite bacteria is a mixture of Bacillus amyloliquefaciens and Bacillus thiaminesolyticus in a mass ratio of 1:1.

[0050] The preparation steps of wolfberry leaf extract are as follows:

[0051] Take wolfberry leaves, add them to 85% v / v ethanol solution, reflux extract for 3 times, each time for 0.5 hour, combine the extracts, filter, cool, then add petroleum ether for extraction 3 times, collect the petroleum ether phase, concentrate and dry to obtain wolfberry leaf extract.

[0052] Preparation of nano composting agent:

[0053] The wolfberry leaf extract, compound bacteria, nano iron oxide and 2-hydroxyethyl picric acid are mixed according to a certain proportion to obtain a nano composting agent.

[0054] Comparative Example 1

[0055] The only difference from Example 1 is that the thiamine-lytic Bacillus is replaced by an equal mass of Bacillus amyloliquefaciens.

[0056] Comparative Example 2

[0057] The only difference from Example 1 is that the preparation steps of wolfberry leaf extract are as follows:

[0058] Add 10 times the weight of water to the washed wolfberry leaves and boil them for 3 times, each time for 1 hour. Combine the decoctions, concentrate and dry to obtain the wolfberry leaf extract.

[0059] Comparative Example 3

[0060] The only difference from Example 1 is that the nano iron oxide is replaced by an equal mass of nano silicon dioxide.

[0061] Comparative Example 4

[0062] The only difference from Example 1 is that 2-hydroxyethylpicramic acid is not added.

[0063] Effect verification example:

[0064] 1. Test group: Take fresh, undehydrated chicken manure (C / N=7.2, VOC content of 172.8 mg / m 3 ) were divided into 8 groups of equal mass (50 kg in each group), namely experimental group 1 to experimental group 3, control group 1 to control group 4 and blank control group.

[0065] 2. Experimental treatment: The experimental groups 1-3 were respectively added with the nano-composting agent prepared in Examples 1-3 (the added amount was 1.2% of the mass of chicken manure); the control groups 1-4 were respectively added with the nano-composting agent prepared in Comparative Examples 1-4 (the added amount was 1.2% of the mass of chicken manure); the blank control group was not added with the nano-composting agent.

[0066] Each group was fermented under film, and the film was lifted for ventilation every 5 hours. The plastic film was removed after 3 days of fermentation. The pile was turned over every 3 days, and the fermentation and composting lasted for 8 days in total.

[0067] After fermentation and composting, the nitrogen content, VOC content and sterilization rate of fecal coliform group in each group were measured. The results are shown in Table 1.

[0068] Table 1

[0069] nitrogen(%) C / N <![CDATA[VOC(mg / m 3 )]]> Fecal coliform bacteria killing rate (%) Experimental Group 1 1.54 18.7 70.21 99.99 Experimental Group 2 1.52 18.5 71.47 99.99 Experimental Group 3 1.52 18.7 72.35 99.99 Control group 1 1.87 10.2 121.26 98.58 Control group 2 1.62 13.5 110.21 99.85 Control group 3 1.75 11.5 143.57 99.35 Control group 4 1.64 14.9 109.73 99.95 Blank control group 2.41 8.2 166.27 42.33

[0070] The material temperature and odor sensory conditions of each group at different fermentation and maturity days are shown in Tables 2 and 3.

[0071] Table 2

[0072] Fermentation for 3 days Fermentation for 6 days Fermentation 8 days Experimental Group 1 20.5℃ 55.8℃ 37.5℃ Experimental Group 2 20.3℃ 55.6℃ 36.8℃ Experimental Group 3 20.2℃ 55.5℃ 37.2℃ Control group 1 19.5℃ 42.1℃ 34.7℃ Control group 2 20.3℃ 45.2℃ 35.3℃ Control group 3 19.8℃ 46.8℃ 34.2℃ Control group 4 20.1℃ 42.5℃ 33.9℃ Blank control group 18.2℃ 25.8℃ 22.1℃

[0073] Table 3

[0074] Fermentation for 3 days Fermentation for 6 days Fermentation 8 days Experimental Group 1 Strong odor Reduced odor No obvious odor Experimental Group 2 Strong odor Reduced odor No obvious odor Experimental Group 3 Strong odor Reduced odor No obvious odor Control group 1 Strong odor Reduced odor Strong odor Control group 2 Strong odor Reduced odor Strong odor Control group 3 Strong odor Reduced odor Strong odor Control group 4 Strong odor Reduced odor Strong odor Blank control group Strong odor Strong odor Strong odor

[0075] The odor of chicken manure treated with the nano-composting agent of the present invention basically disappears, and the composting agent of the present invention can maintain high-temperature composting for a long time, with the highest temperature reaching about 55°C. The composting is fast and the effect is excellent. The 30-60 days of traditional composting can be shortened to 8-10 days, and the technical effect is remarkable.

[0076] The nanometer composting agent of the present invention significantly reduces the nitrogen content in chicken manure, improves the carbon-nitrogen ratio, and significantly removes volatile organic matter (VOC).

[0077] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A nano composting agent, characterized in that: The raw materials include the following components in parts by weight: 3-4 parts of compound bacteria, 6-8 parts of wolfberry leaf extract, 0.3-0.5 parts of nano iron oxide and 0.5-0.8 parts of 2-hydroxyethyl picric acid; The composite bacteria is a mixture of Bacillus amyloliquefaciens and Bacillus thiamine-lyticus.

2. The nano composting agent according to claim 1, characterized in that The mass ratio of the Bacillus amyloliquefaciens to the Bacillus thiaminesolyticus is 1:1-2.

3. The nano composting agent according to claim 1, characterized in that The deposit number of the Bacillus amyloliquefaciens is CGMCC No.17841; the deposit number of the Bacillus thiaminesolyticus is CCTCCM2020604.

4. The nano composting agent according to claim 1, characterized in that: The preparation method of the wolfberry leaf extract comprises the following steps: The wolfberry leaves are added to ethanol for reflux extraction to obtain an extract, the extract is extracted with petroleum ether, the petroleum ether phase is collected, concentrated and dried to obtain the wolfberry leaf extract.

5. The nano composting agent according to claim 4, characterized in that: The volume concentration of the ethanol is 80%-90%.

6. The method for preparing the nano composting agent according to any one of claims 1 to 5, characterized in that: The following steps are involved: The wolfberry leaf extract, the composite bacteria, the nano iron oxide and the 2-hydroxyethyl picric acid are mixed according to a mass ratio to obtain a nano composting agent.

7. Use of the nano-composting agent as described in any one of claims 1 to 5 in the composting of chicken manure.

8. A method for accelerating the decomposition of chicken manure, characterized in that: The following steps are involved: The nano-composting agent according to any one of claims 1 to 5 is mixed with fresh chicken manure, and the composting is carried out for 8 to 10 days to achieve rapid decomposition of the chicken manure.

9. The method according to claim 8, characterized in that The addition amount of the nano composting agent in fresh chicken manure is 0.8%-1.2%.