Treatment process and application of polygonatum sibiricum rice grains

Abstract

The invention provides a treatment process and application of polygonatum sibiricum rice grains. The polygonatum sibiricum rice grains which are appropriate in mouth feel, strong in taste, rich in nutrition and high in satiety after being eaten are obtained through the processes of nine times of steaming and nine times of processing, extraction, filling and the like.

Description

Technical Field

[0001] The invention belongs to polygonatum rice grains for meal replacement, and particularly relates to a processing technology of the polygonatum rice grains and application thereof. Background Art

[0002] As a medicinal and edible plant, Polygonatum sibiricum has multiple pharmacological effects such as enhancing immunity, lowering blood lipids and blood sugar, and delaying aging. Research on Polygonatum sibiricum mainly focuses on its medicinal value, but as a plant with both medicinal and edible properties, there are few reports on the development of Polygonatum sibiricum in food. Therefore, in order to enhance the market value of Polygonatum sibiricum, this study developed a deep-processed product of Polygonatum sibiricum. It is expected to provide a theoretical basis for the deep processing and development and production of fresh Polygonatum sibiricum. At present, research on Polygonatum sibiricum mainly focuses on its medicinal value: as a specified species of both medicinal and edible properties, the main nutritional components of Polygonatum sibiricum and the changes in its components during the production process are rarely reported.

[0003] Taking raw Polygonatum sibiricum will cause certain irritation, and it will cause certain allergic side effects for sensitive people, thus greatly weakening its own utilization value. Therefore, people will process Polygonatum sibiricum to reduce its irritation on the one hand, and improve its medicinal effect on the other hand. Nowadays, people have improved and perfected the processing methods of Polygonatum sibiricum based on traditional processing experience, mainly including wine processing, steaming, fermentation processing and other methods. The main processing method is still steaming, including single steaming, double steaming, nine steaming and nine processing, steaming with auxiliary materials, etc. There are great differences in processing time, number of times, auxiliary materials, etc. of different processing methods.

[0004] Making Polygonatum odoratum into rice grain shape is not only convenient to carry but also makes full use of the active ingredients in Polygonatum odoratum, taking into account the development of health care and functional food, and improving the comprehensive utilization value of Polygonatum odoratum. The benefit of Polygonatum odoratum as a meal replacement rice lies in its rich nutritional value and lasting satiety, which can provide comprehensive nutritional support for the body and help control calorie intake, achieving the effect of healthy weight loss.

[0005] The carbohydrates in polygonatum include polysaccharides and monosaccharides, which can provide energy and nutrition for the human body. However, after high-temperature steaming, these carbohydrates may degrade or react with free amino acids to produce a variety of heterocyclic substances. These heterocyclic substances have characteristic flavors such as burnt taste, mushy taste, and baked taste, and are the main flavor sources of steamed polygonatum products. The generation of these heterocyclic substances is related to the thermal degradation of carbohydrates and the Maillard reaction. Although these heterocyclic substances have certain nutritional and physiological activities, excessive intake may have an adverse effect on human health. Therefore, controlling the steaming process of the meal-replacing polygonatum rice grains and retaining the effective ingredients to the highest degree is the research focus of the present invention.

[0006] During the industrial steaming process, Polygonatum sibiricum undergoes sensory changes such as blackening, emitting a burnt aroma, and sweetening. This is because it is in a high temperature state for a long time during the processing process, and is affected by the temperature, resulting in non-enzymatic browning reaction and Maillard reaction. During this process, the physical properties and chemical components such as polysaccharides, amino acids, proteins, moisture, and pH value will change. Among them, the rich and high-quality related flavor amino acids may have an impact on the improvement of flavor. The polarity of ethanol may not be sufficient to effectively dissolve 5-HMF and its precursor compounds, resulting in low extraction efficiency. The present invention adds surfactant during the extraction process to improve the extraction effect to obtain a higher content of 5-HMF.

[0007] Sugar alcohols have good antiseptic properties and can effectively extend the shelf life of food. This is especially important for products such as Polygonatum odoratum rice, which is easily affected by moisture and deteriorates, as it can ensure that the product remains fresh and of good quality for a longer period of time. Polygonatum odoratum itself contains certain mucus and starch, which give Polygonatum odoratum rice a unique taste. By adding sugar alcohols, its taste can be further improved, making it more delicate and sweet, thereby increasing consumer acceptance. Summary of the invention

[0008] The present invention discloses a processing technology and application of polygonatum rice grains to solve the above and any potential problems in the prior art. In order to solve the above technical problems, the preparation method of the present invention is:

[0009] The steamed product of nine-steamed and nine-processed polygonatum odoratum, soybeans, and oats that had been washed and removed of impurities were mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve. The mixture was preheated in a wok for 3 minutes, stir-fried until a distinct grain aroma was smelled, poured out, cooled, and crushed. 100 parts of the mixed powder were accurately weighed, 500 parts of the extract were added, vortexed for 30 seconds, homogenized at 45 Hz for 4 minutes, and fully shaken and mixed. The mixture was then placed in an ice-water bath for 1 hour of ultrasound, and placed in a -4°C refrigerator for 1 hour. Place the mixture at 4°C and centrifuge at 12000rpm for 15min, draw the supernatant and filter it through a 0.22μm microporous filter membrane, then apply it to a treated macroporous weakly alkaline anion D392 exchange column, rinse it with 1-3% NaOH solution of 2 times the volume of the resin bed, discard the rinse solution; then rinse it with 3 times the volume of pure water, discard the water rinse solution, elute it with 70-95% ethanol of 7 times the volume of the resin bed, collect the eluate, and dry it to a mixed polygonatum powder with a water content of 6-9%;

[0010] Spray 1-3% of filler into the mixed polygonatum powder obtained by extraction, disperse at 500 rpm for 10 minutes, then punch or extrude in a rice-shaped die to form rice grains, and then dry to obtain polygonatum rice grains;

[0011] The extract is obtained by dissolving a lauryl sulfate triethanolamine solution with a mass fraction of 10-20% in an ethanol solution with a mass fraction of 5-8 times the mass fraction of 70%.

[0012] The nine-steamed and nine-processed polygonatum steamed product is prepared by selecting raw polygonatum without mildew, washing it, removing roots, placing it in a steamer and steaming it for 3 hours using a water-insulating process, steaming it at 100° C. under normal pressure for 2 hours, taking it out and drying it in a 60° C. heat pump dryer to keep its moisture content at 20-30%, thereby obtaining the one-steamed and one-processed polygonatum. This process is repeated nine times to obtain the nine-steamed and nine-processed polygonatum steamed product.

[0013] Wherein, 2-5% ethyl p-methoxycinnamate is added to the water in the steamer.

[0014] The filler is adonit with a mass fraction of 6-12%.

[0015] An application of polygonatum rice grains in meal replacement food is characterized by:

[0016] The application of the above-mentioned Polygonatum rice grains in meal replacement foods is as follows: the Polygonatum rice grains and rice are configured in a ratio of 3:1-2 to obtain the meal replacement Polygonatum rice grains, the meal replacement Polygonatum rice grains are washed 2-3 times, 1.13-2 times of water is added, and steamed for 20-30 minutes.

[0017] The advantages and beneficial effects of the present invention are:

[0018] 1. The present invention provides a processing technology for polygonatum rice grains and an application thereof. Polygonatum rice grains with suitable taste, strong flavor, rich nutrition, good taste, good appearance and strong satiety after eating are obtained through nine-steaming, nine-processing, extraction, filling and other processes.

[0019] 2. The present invention adopts steaming in water, and ethyl p-methoxycinnamate is added to the water as a penetrant, which can help to significantly change the internal structure of Polygonatum odoratum during the process of nine steaming and nine processing, so that the internal tissue structure of Polygonatum odoratum changes from dense to loose and porous. In the process of nine steaming and nine processing, this compound can help break the dense structure of Polygonatum odoratum and make it loose and porous. By improving the structure of Polygonatum odoratum, it is helpful to control the undesirable flavors such as burnt smell and paste smell caused by excessive degradation of sugar substances and Maillard reaction. The loose and porous structure can increase the solubility and dissolvability of food, make the taste of Polygonatum odoratum sweeter, reduce bitterness, improve the overall flavor, and make food easier to be absorbed and used by the human body. This means that Polygonatum odoratum after nine steaming and nine processing may more effectively exert its food effect, and the loose and porous structure is conducive to solvent penetration and extraction of food ingredients. In the process of processing, the time for food to contact with hot water increases, and the solvent can better penetrate into the interior of Polygonatum odoratum, which helps to extract the active ingredients of Polygonatum odoratum later.

[0020] 3. And as the number of steaming increases, the smell becomes stronger and the 5-HMF content increases greatly. At the same time, the overall taste is changed. The initial bitter tongue feeling gradually disappears, and the sweet taste of Polygonatum is improved. It not only removes its bad taste and irritation, but also improves its medicinal effect and shelf life.

[0021] 4. The present invention selects to add lauryl sulfate triethanolamine to the ethanol solution to extract the active substance. Lauryl sulfate triethanolamine, as a derivative of triethanolamine, has an emulsifying property and can stabilize the oil-water mixture. And lauryl sulfate triethanolamine has weak alkalinity, which can destroy the cell wall of polygonatum and change its permeability, so that the polyphenol active ingredients in polygonatum are released, such as polysaccharides, steroidal saponins, triterpenes, alkaloids, flavonoids, phytosterol compounds and volatile oils, etc. Compared with a single ethanol solution, lauryl sulfate triethanolamine can more effectively dissolve and extract the active ingredients in polygonatum, and improve the extraction rate of substances such as 5-hydroxymethylfurfural. The application of lauryl sulfate triethanolamine in ethanol solution not only increases the extraction amount of active substances, but also improves the quality of the extract, and increases the stability and effectiveness of active ingredients in the extraction process. Lauryl sulfate triethanolamine enhances the extraction efficiency by improving the chemical environment during the extraction process, and the extraction effect of active substances is significantly better than traditional ethanol extraction.

[0022] 5. The present invention adds adonit as a filler during the process of forming the meal replacement polygonatum rice grains. Based on the polygonatum extract, adonit as a filler can fill the gaps in the food ingredients, thereby improving the structural stability of the product. In the process of forming the meal replacement polygonatum rice grains, the addition of adonit can help form a uniform structure, so that the polygonatum rice grains are more stable during processing and are not easy to disintegrate or deform. Adonit has good hygroscopicity and can increase the water holding capacity of the product. By absorbing and retaining moisture, adonit can prevent the polygonatum rice grains from being too dry during processing, thereby maintaining the appropriate moisture of the rice grains. This water holding capacity helps to improve the texture and taste of the product. Adonit helps to form a gluten network through interaction with other ingredients. The gluten network is a structure formed by gluten protein, which can enhance the cohesiveness and stability of food. In the polygonatum rice grains, the combination of adonit and other ingredients forms a stable network structure, which helps to improve the crack resistance of the rice grains, making it not easy to break or break during processing and use. The obtained polygonatum rice grains are rich in bioactive ingredients such as polysaccharides, saponins, flavonoids and the like, and provide a lasting feeling of fullness. DETAILED DESCRIPTION

[0023] The present invention will be further described in detail below in conjunction with the embodiments.

[0024] Example 1

[0025] Select raw polygonatum sibiricum without mildew, wash it, remove the roots, put it in a steamer and steam it for 3 hours using a water-bath process, add 3.5% ethyl p-methoxycinnamate into the water in the steamer, steam it at 100°C under normal pressure for 2 hours, take it out and dry it in a heat pump dryer at 60°C so that its moisture content is maintained at 25%, thus obtaining one-steamed and one-processed polygonatum sibiricum, and repeat this process nine times to obtain a nine-steamed and nine-processed polygonatum steamed product.

[0026] Example 2

[0027] Select raw Polygonatum sibiricum without mildew, wash it, remove the roots, put it in a steamer and steam it for 3 hours using a water-bath process, add 2% ethyl p-methoxycinnamate into the water in the steamer, steam it at 100°C under normal pressure for 2 hours, take it out and dry it in a heat pump dryer at 60°C so that its moisture content is maintained at 20%, thus obtaining one-steamed and one-processed Polygonatum sibiricum, and repeat this process nine times to obtain a nine-steamed and nine-processed Polygonatum sibiricum steamed product.

[0028] Example 3

[0029] Select raw Polygonatum sibiricum without mildew, wash it, remove the roots, put it in a steamer and steam it for 3 hours using a water-bath process, add 5% ethyl p-methoxycinnamate to the water in the steamer, steam it at 100°C under normal pressure for 2 hours, take it out and dry it in a heat pump dryer at 60°C so that its moisture content is maintained at 30%, thus obtaining one-steamed and one-processed Polygonatum sibiricum, and repeat this process nine times to obtain a nine-steamed and nine-processed Polygonatum sibiricum steamed product.

[0030] Comparative Example 1

[0031] The difference between this comparative example and Example 1 is that the steaming in this comparative example is distilled water, and the specific process is: select raw polygonatum without mildew, wash and remove roots, put it in a steamer and steam it for 3 hours using a water-insulating process, steam it at 100°C under normal pressure for 2 hours, take it out and dry it in a 60°C heat pump dryer to keep its moisture content at 25%, so as to obtain one-steamed and one-processed polygonatum, and repeat this process nine times to obtain a nine-steamed and nine-processed polygonatum steamed product.

[0032] Comparative Example 2

[0033] The difference between this comparative example and Example 1 is that the ethyl p-methoxycinnamate in this comparative example is methyl gallate, and the rest is the same as Example 1.

[0034] Comparative Example 3

[0035] The difference between this comparative example and Example 1 is that the polygonatum in this comparative example is obtained by steaming and processing: raw polygonatum without mildew is selected, washed, roots are removed, placed in a steamer and steamed for 3 hours using a water-insulating process, 3.5% ethyl p-methoxycinnamate is added to the water in the steamer, steamed at 100°C under normal pressure for 2 hours, and taken out and dried in a heat pump dryer at 60°C to keep its moisture content at 25%, thereby obtaining the steamed and processed polygonatum.

[0036] Comparative Example 4

[0037] The difference between this comparative example and Example 1 is that the addition ratio of ethyl p-methoxycinnamate in distilled water is different, specifically: select raw Polygonatum sibiricum without mildew, wash and remove roots, put it in a steamer and steam it for 3 hours using a water-insulating process, and add 8% ethyl p-methoxycinnamate to the water in the steamer, steam it at 100°C under normal pressure for 2 hours, take it out and dry it in a heat pump dryer at 60°C to keep its moisture content at 25%, so as to obtain one-steamed and one-processed Polygonatum sibiricum, and repeat this process nine times to obtain a nine-steamed and nine-processed Polygonatum sibiricum steamed product.

[0038] Comparative Example 5

[0039] The difference between this comparative example and Example 1 is that the addition ratio of ethyl p-methoxycinnamate in distilled water is different, specifically: select raw Polygonatum sibiricum without mildew, wash and remove roots, put it in a steamer and steam it for 3 hours using a water-insulating process, and add 1% ethyl p-methoxycinnamate to the water in the steamer, steam it at 100°C under normal pressure for 2 hours, take it out and dry it in a heat pump dryer at 60°C to keep its moisture content at 25%, so as to obtain one-steamed and one-processed Polygonatum sibiricum, and repeat this process nine times to obtain a nine-steamed and nine-processed Polygonatum sibiricum steamed product.

[0040] Experiment 1: Determination of the content of Polygonatum odoratum in nine-steamed and nine-processed

[0041] The above-mentioned Polygonatum sibiricum product was crushed, 0.1 g of Polygonatum sibiricum powder was accurately weighed, 2 mL of ultrapure water was added, vortexed and mixed, and then crushed with a cell crusher at 8000 rmin for 1 min. The supernatant was taken and filtered through a 0.45 μm microporous aqueous phase filter membrane as the Polygonatum sibiricum test solution.

[0042] Reducing sugar determination: Prepare glucose standard solution (1.00 mg / mL), draw 0.00, 0.10, 0.20, 0.30, 0.40, 0.50, 0.60 mL into a test tube, add deionized water to 1 mL, add 2 mL DNS reagent, boil accurately in a boiling water bath for 5 min, take out, cool the tap water to room temperature, add 9 mL distilled water, and measure the absorbance of each tube at 540 nm. Take 1 mL of the test solution of Polygonatum odoratum and determine the reducing sugar content according to the following formula.

[0043] Chromatographic column: Shodex NH2P-50-4E (4.6mmX250mm, 5μm), guard column: NH2P-50G4A (4.6mmX10mm), mobile phase is water (phase A) and acetonitrile (phase B), A:B=25:75, flow rate: 0.8mL / min, column temperature: 30℃, injection volume 5μL, ELSD parameters: tube temperature=40℃, carrier gas flow rate=2.5L / min.

[0044]

[0045] Where: C is the amount of sugar found from the standard curve, mg; VT is the volume of the extract, mL; m is the mass of the plant sample, mg; VS is the sample volume used in the determination, mL.

[0046] Determination of total phenol content: Accurately weigh 1g of Polygonatum sibiricum powder, add 80% ethanol at a ratio of 1:15 (g / mL), ultrasonicate for 40 minutes, centrifuge and take the supernatant, repeat twice, combine the supernatants and wait for measurement. The total phenol content is determined by the Folin-Ciocalten method, with gallic acid as the standard, and a 96-well microplate is selected for determination. 25μL of gallic acid standard solution or sample solution is added to a 96-well plate, and then 125μL of Folin-phenol reagent (0.2molL) is added to react at room temperature for 10 minutes, and then 125μL of saturated Na2CO3 solution (10g / 100mL) is added, and the mixture is slowly shaken on an oscillator and placed at room temperature for reaction for 30 minutes, and then the absorbance is measured at 765nm. The concentrations of gallic acid standard solutions were 10, 20, 40, 80, 120, 160, and 200 mg / L. The total phenol content of the samples was expressed as mg gallic acid / g dry weight (mg GAE / g DW).

[0047] Determination of total ketone content: Weigh a certain amount of polygonatum powder, add 20 times the volume of 80% ethanol solution, ultrasonicate at 70°C for 1 hour, let it stand for 1.5 hours (shake once every 10 minutes), and extract twice. Determine the flavonoid content.

[0048] Table 1 Determination of Polygonatum sibiricum Content

[0049] Group Reducing sugar content (%) Total phenol content (%) Total ketone content (%) Example 1 35.8 14.9 1.3 Example 2 34.9 14.7 1.2 Example 3 34.5 14.5 1.2 Comparative Example 1 27.5 10.8 0.8 Comparative Example 2 30.4 11.7 1.0 Comparative Example 3 4.8 0.8 0.1 Comparative Example 4 33.7 13.6 1.1 Comparative Example 5 31.7 12.4 1.0

[0050] The following examples are all carried out based on the following steps in Example 1.

[0051] Example 4

[0052] The steamed product of nine-steamed and nine-processed polygonatum odoratum that had been washed and impurities removed, soybeans, and oats were mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve, put into a wok and preheated for 3 minutes, stir-fried until a distinct cereal aroma was smelled, poured out, cooled, and crushed, 100 parts of the mixed powder were accurately weighed, and 500 parts of the extract were added, the extract was obtained by dissolving a 15% by mass fraction of triethanolamine lauryl sulfate solution in 6 times the mass fraction of 70% ethanol solution, vortexed for 30 seconds, homogenized at 45Hz for 4 minutes, fully shaken and mixed, and then Place in an ice-water bath for ultrasonic treatment for 1 hour, and place in a -4°C refrigerator for 1 hour. Centrifuge the sample at 4°C and 12,000 rpm for 15 minutes. Pipette the supernatant and filter it through a 0.22 μm microporous filter membrane. Apply it to a treated macroporous weakly alkaline anion D392 exchange column and rinse it with 2% NaOH solution (2 times the volume of the resin bed), and discard the rinse solution; rinse it with 3 times pure water, and discard the water rinse solution. Elute it with 82% ethanol (7 times the volume of the resin bed), collect the eluate, and dry it to a mixed Polygonatum odoratum powder with a moisture content of 7%.

[0053] 2% of 8% adonit by mass was sprayed into the mixed polygonatum powder obtained by extraction, and the mixture was dispersed at 500 rpm for 10 minutes, and then punched or extruded into rice grains in a knife die in the shape of rice grains, and then dried to obtain polygonatum rice grains.

[0054] Example 5

[0055] The steamed nine-steamed and nine-processed polygonatum odoratum product that had been washed and impurities removed, soybeans, and oats were mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve, put into a wok and preheated for 3 minutes, stir-fried until a distinct cereal aroma was smelled, poured out, cooled, and crushed, 100 parts of the mixed powder were accurately weighed, and 500 parts of the extract were added, the extract being obtained by dissolving a 10% by mass fraction of triethanolamine lauryl sulfate solution in 8 times the mass fraction of 70% ethanol solution, vortexed for 30 seconds, homogenized at 45Hz for 4 minutes, fully shaken and mixed, and then Place in an ice-water bath for ultrasonic treatment for 1 hour, and place in a -4°C refrigerator for 1 hour. Centrifuge the sample at 4°C and 12000rpm for 15 minutes. Pipette the supernatant and filter it through a 0.22μm microporous filter membrane. Apply it to a treated macroporous weakly alkaline anion D392 exchange column and rinse it with 2 times the volume of the resin bed and 3% NaOH solution. Discard the rinse solution. Rinse it with 3 times the volume of pure water and discard the rinse solution. Elute it with 70% ethanol with 7 times the volume of the resin bed. Collect the eluate and dry it to a mixed Polygonatum odoratum powder with a water content of 9%.

[0056] 3% of 6% adonit by mass is sprayed into the mixed polygonatum powder obtained by extraction, and the mixture is dispersed at 500 rpm for 10 minutes, and then stamped or extruded in a rice-shaped die to form rice grains, and then dried to obtain polygonatum rice grains.

[0057] Example 6

[0058] The steamed product of nine-steamed and nine-processed polygonatum odoratum that had been washed and impurities removed, soybeans, and oats were mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve, put into a wok and preheated for 3 minutes, stir-fried until a distinct cereal aroma was smelled, poured out, cooled, and crushed, 100 parts of the mixed powder were accurately weighed, and 500 parts of the extract were added, the extract was obtained by dissolving 20% ​​by mass of triethanolamine lauryl sulfate solution in 5 times the mass of 70% ethanol solution, vortexed for 30 seconds, homogenized at 45Hz for 4 minutes, fully shaken and mixed, and then Place in an ice-water bath for ultrasonic treatment for 1 hour, and place in a -4°C refrigerator for 1 hour. Centrifuge the sample at 4°C and 12000rpm for 15 minutes. Pipette the supernatant and filter it through a 0.22μm microporous filter membrane. Apply it to a treated macroporous weak alkaline anion D392 exchange column and rinse it with 1% NaOH solution (2 times the volume of the resin bed), and discard the rinse solution; rinse it with 3 times the volume of pure water, and discard the water rinse solution. Elute it with 95% ethanol (7 times the volume of the resin bed), collect the eluate, and dry it to a mixed Polygonatum odoratum powder with a water content of 6%.

[0059] 1% of 12% adonitol by mass is sprayed into the mixed polygonatum powder obtained by extraction, and the mixture is dispersed at 500 rpm for 10 minutes, and then stamped or extruded in a rice-shaped die to form rice grains, and then dried to obtain polygonatum rice grains.

[0060] Comparative Example 6

[0061] The difference between this comparative example and Example 4 is that the extracting solution in this comparative example is a 70% ethanol solution, and the rest is the same as Example 4.

[0062] Comparative Example 7

[0063] The difference between this comparative example and Example 4 is that the lauryl sulfate triethanolamine solution in this comparative example is sodium dodecyl sulfate, and the rest is the same as Example 4.

[0064] Comparative Example 8

[0065] The difference between this comparative example and Example 4 is that the amount of the triethanolamine lauryl sulfate solution added in this comparative example is different, specifically: the steamed product of nine-steamed and nine-processed polygonatum odoratum that has been washed and impurities removed, soybeans, and oats are mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve, put into a wok and preheated for 3 minutes, stir-fried until a distinct cereal aroma is smelled, poured out, cooled, and crushed, 100 parts of the mixed powder are accurately weighed, 500 parts of the extract are added, and the extract is obtained by dissolving a 15% by mass fraction triethanolamine lauryl sulfate solution in 3 times the mass fraction of a 70% ethanol solution, vortexed for 30 seconds, 45Hz The mixture was homogenized for 4 min and thoroughly vortexed to mix. The mixture was then placed in an ice-water bath for 1 h for ultrasonication. After standing in a -4°C refrigerator for 1 h, the sample was centrifuged at 4°C and 12,000 rpm for 15 min. The supernatant was filtered through a 0.22 μm microporous membrane and then applied to a treated macroporous weakly alkaline anion D392 exchange column. The column was rinsed with 2% NaOH solution (2 times the volume of the resin bed) and the rinse was discarded. The column was then rinsed with 3 times pure water and the rinse was discarded. The column was eluted with 82% ethanol (7 times the volume of the resin bed) and the eluate was collected and dried to a mixed polygonatum powder with a water content of 7%. The rest was the same as in Example 4.

[0066] Comparative Example 9

[0067] The difference between this comparative example and Example 4 is that the amount of the triethanolamine lauryl sulfate solution added in this comparative example is different, specifically: the steamed product of nine-steamed and nine-processed polygonatum odoratum that has been washed and impurities removed, soybeans, and oats are mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve, put into a wok and preheated for 3 minutes, stir-fried until a distinct cereal aroma is smelled, poured out, cooled, and crushed, 100 parts of the mixed powder are accurately weighed, 500 parts of the extract are added, and the extract is obtained by dissolving a 15% by mass fraction triethanolamine lauryl sulfate solution in 10 times the mass fraction of a 70% ethanol solution, vortexed for 30 seconds, 45Hz The mixture was homogenized for 4 min and thoroughly vortexed to mix. The mixture was then placed in an ice-water bath for 1 h for ultrasonication. After standing in a -4°C refrigerator for 1 h, the sample was centrifuged at 4°C and 12,000 rpm for 15 min. The supernatant was filtered through a 0.22 μm microporous membrane and then applied to a treated macroporous weakly alkaline anion D392 exchange column. The column was rinsed with 2% NaOH solution (2 times the volume of the resin bed) and the rinse was discarded. The column was then rinsed with 3 times pure water and the rinse was discarded. The column was eluted with 82% ethanol (7 times the volume of the resin bed) and the eluate was collected and dried to a mixed polygonatum powder with a water content of 7%. The rest was the same as in Example 4.

[0068] Comparative Example 10

[0069] The difference between this comparative example and Example 4 is that the adonit in this comparative example is xylitol, and the rest is the same as Example 4.

[0070] Comparative Example 11

[0071] The difference between this comparative example and Example 4 is that the amount of adonit added in this comparative example is different, specifically: 2% of adonit with a mass fraction of 15% is sprayed into the mixed polygonatum powder obtained by extraction, and after dispersing at 500 rpm for 10 minutes, it is punched or extruded into a rice grain-shaped die to form rice grains, and then dried to obtain polygonatum rice grains; the rest is the same as Example 4.

[0072] Comparative Example 12

[0073] The difference between this comparative example and Example 4 is that the amount of adonit added in this comparative example is different, specifically: 2% of adonit with a mass fraction of 3% is sprayed into the mixed polygonatum powder obtained by extraction, and after dispersing at 500 rpm for 10 minutes, it is punched or extruded into a rice grain-shaped die to form rice grains, and then dried to obtain polygonatum rice grains; the rest is the same as Example 4.

[0074] Test 2:

[0075] Determination of 5-HMF content:

[0076] Solution preparation: Accurately weigh an appropriate amount of 5-HMF reference substance and place it in a 5 mL volumetric flask, add methanol to dissolve and dilute to the scale, shake well to make a solution containing 7.9 mg / L as the reference solution, accurately weigh 0.5 mL of the extract of Examples 4-6 and Comparative Examples 6-9, evaporate the solvent, make up the residue to volume in a 5 mL volumetric flask with methanol, and filter with a 0.45 m microporous filter membrane as the test solution.

[0077] Selection of chromatographic conditions: Chromatographic column: Diam onsiL G 18 (5μL200x4.6mm); the mobile phase was acetonitrile: ice (15:85), the flow rate was 1mL / min; the column temperature was 25°C, the measurement wavelength was 280nm, and the injection volume was 20μL. Under the above conditions, the results of the measurement of 5-HMF are shown in Table 2 below.

[0078] Table 25-HMF content

[0079] Group 5-HMF content / mg Example 4 36.78 Example 5 35.96 Example 6 35.85 Comparative Example 6 30.44 Comparative Example 7 31.78 Comparative Example 8 34.52 Comparative Example 9 32.83

[0080] Test 3:

[0081] The integrity of the Polygonatum sibiricum rice grains obtained in Examples 4-6 and Comparative Examples 10-12 was tested. 300 g of sample was taken from each group and placed in a 14-mesh stainless steel vibrating sieve, model ZSG1443, for 8 min at a frequency of 200 times / min. The mass of the Polygonatum sibiricum rice grains before and after sieving and the mass of the incomplete and broken Polygonatum sibiricum rice grains were weighed to calculate the integrity.

[0082]

[0083] The results are shown in Table 3.

[0084] Table 3

[0085]

Claims

1. A processing process for polygonatum rice grains, characterized in that: The processing technology comprises: The steamed product of nine-steamed and nine-processed polygonatum odoratum that had been washed and impurities removed, soybeans, and oats were mixed in a ratio of 3:1:1, dried, crushed, and passed through a 60-mesh sieve. The mixture was preheated in a wok for 3 minutes, stir-fried until a distinct cereal aroma was smelled, poured out, cooled, and crushed. 100 parts of the mixed powder were accurately weighed, 500 parts of the extract were added, vortexed for 30 seconds, homogenized at 45 Hz for 4 minutes, and fully shaken and mixed. The mixture was then placed in an ice-water bath for 1 hour for ultrasonication. After standing in a -4 ℃ refrigerator for 1 hour, the sample was placed at 4 ℃ and centrifuged at 12000 rpm for 15 minutes. The supernatant was aspirated and filtered through a 0.22 μm Filter with a microporous membrane, then place on a treated macroporous weak alkaline anion exchange column D392, rinse with 1-3% NaOH solution twice the volume of the resin bed, discard the rinse; then rinse with 3 times the volume of pure water, discard the water rinse, elute with 70-95% ethanol seven times the volume of the resin bed, collect the eluate, and dry it to a mixed polygonatum powder with a water content of 6-9%; Spray 1-3% of filler into the mixed polygonatum powder obtained by extraction, disperse at 500 rpm for 10 minutes, then punch or extrude in a die with a rice grain shape to form rice grains, and then dry to obtain polygonatum rice grains; The extract is obtained by dissolving 10-20% by mass of a lauryl sulfate triethanolamine solution in 5-8 times the mass of a 70% by mass ethanol solution.

2. The processing process of a polygonatum rice grain according to claim 1, characterized in that: The preparation method is: The nine-steamed and nine-processed polygonatum steamed product is prepared by selecting raw polygonatum without mildew, washing it, removing roots, placing it in a steamer and steaming it for 3 hours using a water-insulating process, steaming it at 100° C. under normal pressure for 2 hours, taking it out and drying it in a 60° C. heat pump dryer to keep its moisture content at 20-30%, thereby obtaining the one-steamed and one-processed polygonatum. This process is repeated nine times to obtain the nine-steamed and nine-processed polygonatum steamed product.

3. The processing technology of a kind of polygonatum rice grains according to claim 2 is characterized in that: in, Add 2-5% ethyl p-methoxycinnamate to the water in the steamer.

4. The processing technology of a kind of polygonatum rice grains according to claim 1 is characterized in that: The filler is adonit with a mass fraction of 6-12%.

5. An application of polygonatum rice grains prepared by the processing process of any one of claims 1 to 4 in meal replacement foods, characterized in that: The application of the above-mentioned polygonatum rice grains in meal replacement food is as follows: the polygonatum rice grains and rice are configured in a ratio of 3:1-2 to obtain the meal replacement polygonatum rice grains, the meal replacement polygonatum rice grains are washed 2-3 times, 1.13-2 times of water is added, and steamed for 20-30 minutes.

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