A glycoside derivative flexible liposome, its preparation method and application

Abstract

The present invention belongs to the field of personal care, and particularly relates to a glycoside derivative flexible liposome, a preparation method and an application thereof. The glycoside derivative flexible liposome comprises a main shell material, a humectant, a raw material to be encapsulated, an auxiliary shell material, a preservative and a solvent. The main shell material is phosphatidylcholine, and the auxiliary shell material is polyquaternium-51. The preservative is any one or more of 1,2-hexanediol, p-hydroxyacetophenone, methylparaben, phenoxyethanol, and caprylyl glycol. The humectant is butanediol. The raw material to be encapsulated is hydroxypropyltetrahydrofurantriol. The solvent is deionized water. The present invention uses a flexible liposome encapsulation technology to improve the permeability and penetration depth of boswellia serrata extract and its concentration at the target site, improve the bioavailability of boswellia serrata extract, reduce the addition amount under the condition of achieving the same firming and anti-wrinkle effect, and thus reduce the cost.

Description

Technical Field

[0001] The present invention belongs to the field of personal care, and particularly relates to a glycoside derivative flexible liposome, a preparation method thereof, and an application thereof. Background Art

[0002] With the increase of age, the expression of glycosaminoglycans (GAGs) and proteoglycans (HS-PGs) in the extracellular matrix of the skin gradually decreases, the collagen in the skin is lost, the cohesion of the basement membrane layer (DEJ layer), which is the connecting layer between the epidermis and the dermis, decreases, and the epidermis and the dermis cannot be well connected, reducing the signal communication and nutrient transport between the epidermis and the dermis inside the skin, resulting in gradual skin aging, the appearance of wrinkles, and the loss of elasticity and firmness. Pro-Xylane (hydroxypropyltetrahydropyrantriol) is an anti-aging active ingredient that targets the above three major skin aging targets. It is a xylose derivative formed by the combination of xylose and a glycosyl ligand. Pro-Xylane can promote the expression of collagen I in the dermis and delay the loss of collagen in the skin; Pro-Xylane is also an activator of glycosaminoglycans, which can provide binding sites for the synthesis of glycosaminoglycans, promote the generation of glycosaminoglycans, and further promote the generation of proteoglycans. It has a promoting effect on three forms of proteoglycans, namely beaded proteoglycan, hyaluronic acid receptor (CD44), and syndecan. Glycosaminoglycans and proteoglycans (HS-PGs), which are important components of the extracellular matrix in the skin, can absorb a large amount of water, adhere to surrounding cells, and form an elastic colloidal body, supporting the fullness of the skin, thereby achieving the effects of anti-wrinkle and moisturizing.

[0003] In addition, Pro-Xylane also promotes the synthesis of collagen VII and collagen IV. Collagen VII and collagen IV are located in the dermo-epidermal junction (DEJ layer), which can make the epidermis and the dermis connect more tightly, and can also make the skin better resist gravity and other mechanical stresses, making the skin more firm and elastic.

[0004] Pro-Xylane may promote the renewal of keratinocytes by activating growth factors and interacting with growth factors, or may regulate epidermal homeostasis through the release of growth factors during skin aging. As a mild and effective anti-aging active ingredient, Pro-Xylane is widely used in cosmetics, but some application pain points have also been found in its application: 1. Sufficient amounts of Pro-Xylane need to be added to achieve good anti-wrinkle and firming effects, but a high addition amount leads to a decrease in absorption efficiency and poor skin feel experience; 2. The penetration efficiency of Pro-Xylane is low and the bioavailability is not high; 3. The main anti-wrinkle targets of Pro-Xylane are located in the dermo-epidermal junction and the dermis. Generally, the transdermal penetration depth and the effective concentration at the target position of Pro-Xylane are difficult to reach the expected value, resulting in low bioavailability, slow onset of anti-aging effects, and insignificant anti-aging effects.

[0005] In view of the above application pain points, there is an urgent need for a product that encapsulates hydroxyprolisone, which is stable, has a good skin feel, a high transdermal absorption rate, a high bioavailability, can balance efficacy and cost, and is more cost-effective. Summary of the Invention

[0006] In view of the deficiencies in the prior art, the present invention provides a glycoside derivative flexible liposome with moisturizing and anti-wrinkle functions, a preparation method thereof, and an application thereof, which can improve skin roughness, smooth and delicate the skin, and enhance the absorption of active ingredients by the skin.

[0007] The present invention adopts the following technical solutions:

[0008] A preparation method of a glycoside derivative flexible liposome, wherein the glycoside derivative flexible liposome comprises a main shell material, a moisturizer, an encapsulated raw material, an auxiliary shell material, a preservative, and a solvent; the main shell material is phosphatidylcholine; the auxiliary shell material is polyquaternium-51; the preservative is any one or more of 1,2-hexanediol, p-hydroxyacetophenone, methylparaben, phenoxyethanol, and caprylyl glycol; the moisturizer is butanediol; the encapsulated raw material is hydroxypropyltetrahydrofurantriol; the solvent is deionized water;

[0009] The preparation method comprises the following steps: heating the main shell material and the moisturizer in a water bath to 45-75 °C and stirring evenly; then adding the encapsulated raw material preheated to the same temperature and stirring evenly to obtain a mixture A; preheating the auxiliary shell material and the preservative to the same temperature and then adding them to the mixture A and continuously stirring to obtain a mixture B. After cooling, injecting the solvent into the mixture B, and continuously hydrating after the injection to obtain a primary emulsion; homogenizing the primary emulsion to obtain the glycoside derivative flexible liposome, and the particle size of the glycoside derivative flexible liposome is 10-40 nm; controlling the discharge temperature of the first homogenization to be 20-30 °C, the discharge temperature of the second homogenization to be 15-25 °C, and the discharge temperature of the third homogenization to be 10-20 °C.

[0010] Further, the glycoside derivative flexible liposome comprises the following components in parts by mass: 2-8 parts of the main shell material, 6-14 parts of the moisturizer, 25-35 parts of the encapsulated raw material, 0.3-3 parts of the auxiliary shell material, 1-2 parts of the preservative, and 38-65.7 parts of the solvent.

[0011] Further, after obtaining the mixture B, cooling it to 35-45 °C; injecting the solvent preheated to 35-45 °C into the mixture B.

[0012] Further, controlling the discharge temperature of the first homogenization to be 25 °C, the discharge temperature of the second homogenization to be 20 °C, and the discharge temperature of the third homogenization to be 15 °C.

[0013] Further, during the process of injecting the solvent into mixture B, stir continuously at a speed of 600 - 800 rpm. After the injection is completed, continue the hydration for 15 - 25 min to obtain the primary emulsion.

[0014] Further, homogenize the primary emulsion with a high-pressure homogenizer, set the homogenization pressure to 12000 - 15000 psi, and the number of homogenization cycles to 3 times.

[0015] The present invention also provides a glycoside derivative flexible liposome prepared by the above method.

[0016] The present invention also provides the application of the above glycoside derivative flexible liposome in personal care products.

[0017] Further, the personal care product is a skin care product.

[0018] Further, the mass percentage of the glycoside derivative flexible liposome in the personal care product is 1 - 3%.

[0019] Beneficial effects

[0020] The glycoside derivative flexible liposome of the present invention is an efficient and mild moisturizing and anti-wrinkle raw material, with excellent water-locking and moisturizing properties. It can form a breathable biofilm on the skin surface, simulate the stratum corneum to give the skin an extra layer of protection, reduce the irritation of surfactants in skin care products, improve skin roughness, smooth and delicate the skin, and enhance the skin's absorption of active ingredients.

[0021] Among them, the phospholipid raw materials used in the liposome have certain moisturizing and antioxidant effects, good hydrophilicity and lipophilicity. After use, they will add luster to the skin. The flexible liposome prepared with it as the main shell material has advantages such as low toxicity, high permeability, and high skin compatibility, and can also bring a better skin feel, and can promote the renewal and repair of skin cells.

[0022] Polyquaternium-51 is a copolymer composed of 2-methacryloyloxyethyl phosphorylcholine and the monomer hydrophobic butyl methacrylate. It is a living body affinity raw material designed by mimicking the cell membrane and can inhibit the stimulation of various surfactants on living bodies and cells. Compared with ordinary polyquaternium salts, it has a unique biomimetic cell membrane structure and living body affinity. Polyquaternium-51 can form a stable hydrated gel film on the skin surface to lock in moisture and prevent moisture evaporation, and it is a high molecular weight moisturizer. At the same time, polyquaternium-51 has a cell membrane-like structure, which can alleviate and inhibit the irritation of surfactants to the skin. In addition to moisturizing and reducing surfactant irritation, polyquaternium-51 also has various effects such as improving skin roughness, smoothing and delicate the skin, and enhancing the skin's absorption of active ingredients.

[0023] The present invention innovatively uses polyquaternium-51 as an auxiliary shell material, which has cationicity and can attract negatively charged skin cells, helping with penetration and absorption; it can also endow flexible liposomes with better deformation ability, achieving a penetration efficiency superior to that of general liposomes. Under non-occlusive conditions, when the flexible liposomes are evenly applied to the skin, the evaporation of water therein will form a hydration penetration gradient from the outside to the inside on the skin surface, pulling the flexible liposomes to move into the skin along the pores, thereby increasing the transdermal absorption of the active substance.

[0024] Polyquaternium-51 cooperates with phospholipid raw materials, having good compatibility and stability, and achieving the stability of hydroxyprogesterone caproate without adding external antioxidants such as sodium metabisulfite and vitamin E acetate.

[0025] The glycoside derivative flexible liposomes encapsulated by the preparation method of the present invention have good stability under high and low temperature conditions; it solves the problems of decreased absorption efficiency and sticky skin feeling caused by the high addition amount required for high anti-aging efficacy; by using the encapsulation technology, it improves the permeability, penetration depth and concentration at the target position of hydroxyprogesterone caproate, improves the bioavailability of hydroxyprogesterone caproate, and reduces the addition amount under the condition of achieving the same firming and anti-wrinkle efficacy, thereby reducing costs. Description of the Drawings

[0026] Figure 1 It is the particle size test data 24 hours after the preparation of Example 1;

[0027] Figure 2 It is the appearance diagrams at 24 hours and 30 days under different storage conditions of Example 1;

[0028] Figure 3 It is the comparison diagram of the cumulative penetration amount of hydroxyprogesterone caproate;

[0029] Figure 4 It is the summary table of the immunofluorescence results of active collagen type I. Photographed with a fluorescence microscope (Olympus, BX43), the magnification is 20×, the blue is the cell nucleus, the green fluorescence is active collagen type I, and the stronger the green fluorescence intensity, the more the content of active collagen type I;

[0030] Figure 5 It is the bar chart of the relative integrated optical density (IOD) value of active collagen type I. When performing statistical analysis using the t-test method, compared with the BC group, the significance is indicated by #, P < 0.05 is indicated by #, P < 0.01 is indicated by ##; compared with the NC group, the significance is indicated by *, P < 0.05 is indicated by *, P < 0.01 is indicated by **, compared with milpupin-2%, the significance is indicated by ◆, P < 0.05 is indicated by ◆, P < 0.01 is indicated by ◆◆;

[0031] Figure 6It is a summary table of the immunofluorescence results of active type IV collagen; taken with a fluorescence microscope (Olympus, BX43) at a magnification of 20×. The blue color represents the cell nucleus, and the green fluorescence represents active collagen. The stronger the green fluorescence intensity, the more the content of active collagen.

[0032] Figure 7 It is a bar graph of the relative integrated optical density (IOD) / area mean of active type IV collagen. Detailed implementation mode

[0033] The present invention provides a glycoside derivative flexible liposome with moisturizing and anti-wrinkle functions, including a main shell material, a moisturizer, a raw material to be encapsulated, an auxiliary shell material, a preservative, and a solvent; the mass parts of the above components are as follows:

[0034] Table 1 Composition range of glycoside derivative flexible liposome

[0035]

[0036] The main shell material is any one or more of lecithin, phosphatidylserine, and phosphatidylcholine.

[0037] The auxiliary shell material is polyquaternium-51.

[0038] The preservative is any one or more of 1,2-hexanediol, p-hydroxyacetophenone, methylparaben, and octyldecanol.

[0039] The moisturizer is butanediol; the raw material to be encapsulated is hydroxypropyltetrahydrofurantriol; the solvent is deionized water.

[0040] Table 2 Compositions of Examples 1-3:

[0041]

[0042] Compositions of Comparative Examples 1-6:

[0043] Comparative Example 1: The auxiliary shell material was not added, and the other components and contents were the same as those in Example 1;

[0044] Comparative Example 2: The auxiliary shell material was 1.0 part by mass of sodium bis(lauroylglutamyl)lysine, and the other components and contents were the same as those in Example 1;

[0045] Comparative Example 3: The auxiliary shell material was 0.5 part by mass of cholesterol and 0.5 part by mass of ceramide, and the other components and contents were the same as those in Example 1;

[0046] Comparative Example 4: The auxiliary shell material was 1.0 part by mass of sodium polylysine, and the other components and contents were the same as those in Example 1;

[0047] Comparative Examples 5-6: The components and contents were the same as those in Example 1.

[0048] Preparation processes of Examples 1-3 and Comparative Examples 1-6:

[0049] Example 1

[0050] Weigh the main shell material and the moisturizer in proportion and heat and stir them evenly in a water bath at 45-75 °C. Then add the raw material to be encapsulated that has been preheated in a water bath at 45-75 °C for 30 min, and stir for 10 min to obtain a uniform mixture A. Preheat the auxiliary shell material and the preservative in a water bath at 45-75 °C for 30 min, and then add them to mixture A and continuously stir for 10 min until uniform to obtain mixture B. Then cool it down to 35-45 °C. Subsequently, inject the solvent preheated in a water bath at 35-45 °C for 30 min into mixture B at a rate of 1 g / min / 100 g of the total system, and continuously stir at a speed of 600-800 rpm during the injection process. After the injection is completed, continue to hydrate for 20 min to obtain the primary emulsion. Finally, homogenize the primary emulsion with a microfluidic homogenizer, set the homogenization pressure to 14000 psi, the number of homogenization cycles to 3 times, control the discharge temperature of the first pass of homogenization to be 25 °C, the discharge temperature of the second pass of homogenization to be 20 °C, and the discharge temperature of the third pass of homogenization to be 15 °C to obtain the material.

[0051] Example 2

[0052] The previous preparation process is the same as that of Example 1. When homogenizing, set the homogenization pressure to 12000 psi, the number of homogenization cycles to 3 times, control the discharge temperature of the first pass of homogenization to be 20 °C, the discharge temperature of the second pass of homogenization to be 15 °C, and the discharge temperature of the third pass of homogenization to be 10 °C to obtain the material.

[0053] Example 3

[0054] The previous preparation process is the same as that of Example 1. When homogenizing, set the homogenization pressure to 15000 psi, the number of homogenization cycles to 3 times, control the discharge temperature of the first pass of homogenization to be 30 °C, the discharge temperature of the second pass of homogenization to be 25 °C, and the discharge temperature of the third pass of homogenization to be 20 °C to obtain the material.

[0055] Comparative Examples 1-4

[0056] The preparation process is the same as that of Example 1.

[0057] Comparative Example 5

[0058] The previous preparation process is the same as that of Example 1. When homogenizing, set the homogenization pressure to 10000 psi, cycle 3 times, and do not control the discharge temperature.

[0059] Comparative Example 6

[0060] The pre - preparation process is the same as that of Example 1. When homogenizing, the homogenization pressure is set at 15000 psi, and it is circulated once. The discharging temperature is controlled at 30 °C, and then it is naturally cooled to obtain the material body.

[0061] Example 4

[0062] Stability performance tests of Examples 1 - 3 and Comparative Examples 1 - 6

[0063] After the preparations of Examples 1 - 3 and Comparative Examples 1 - 6 are completed, appropriate amounts are respectively placed in sealed transparent containers and stored at - 20 °C, room temperature (25 °C), 4 °C, and 45 °C for 3 months each. Examples 1 - 3 are always light - yellow transparent liquids without stratification or precipitation; Comparative Example 1 is a light - blue transparent liquid, which deepens at high temperature after 3 months; Comparative Example 2 is initially a relatively transparent liquid, with precipitation at high temperature and 4 °C. The precipitate may be due to the incompatibility between the amino acid surfactant and hydroxyprolisone; Comparative Example 3 is initially a semi - transparent liquid, becoming turbid at all temperatures. It may be that the ceramide has poor solubility and easily self - assembles and crystallizes out; Comparative Examples 4 - 6 are all light - blue transparent liquids. After 3 months, the transparency decreases at room temperature and high temperature. For Comparative Example 4, it may be due to the relatively high proportion of sodium polylysine leading to instability. Further measurement shows that the initial average particle size of Comparative Example 5 is 54.82 nm, and the polydispersity index (PDI) is 0.393. The initial average particle size of Comparative Example 6 is measured to be 68.23 nm, and the polydispersity index (PDI) is 0.412. For Comparative Examples 5 and 6, the instability may be caused by insufficient homogenization pressure and the lack of gradient cooling of the discharging temperature, resulting in relatively large and non - uniform liposome particle sizes.

[0064] Example 5

[0065] Particle size, PDI and hydroxyprolisone content tests

[0066] The product of Example 1 (subsequently replaced by "Mipuyin" for all) is prepared into a 1% aqueous dispersion, aged at room temperature for 24 h. After 24 h, the appearance of the sample is initially observed, and particle size and PDI tests are carried out.

[0067] The results are as Figure 1 shown. The number - average particle size of Mipuyin at a concentration of 1% is measured to be (23.25 ± 0.80) nm, the PDI is 0.299, and PDI < 0.300, indicating that the Mipuyin prepared by the method of the present invention has a small particle size, a narrow particle - size distribution, an average diameter of 10.48 nm, a peak standard deviation of 3.25 nm, and the flexible liposomes are of uniform size and have good stability.

[0068] Example 6

[0069] Stability test

[0070] The milipin was prepared into a 1% aqueous dispersion, aged at room temperature for 24 h, and then stored at 45 °C, 4 °C, -20 °C, and room temperature (RT), respectively. After 24 h and 30 days of storage, the particle size, polydispersity index (PDI), pH, and content of hydroxyproline were measured, and the appearance was observed.

[0071] When testing, a 1% aqueous dispersion of milipin was used. The average particle size and PDI were measured by a nanoparticle size analyzer at room temperature, and the pH value was measured using a 10% aqueous dispersion of milipin. The content of hydroxyproline in milipin was quantitatively analyzed by HPLC-ELSD.

[0072] The stability was evaluated by comprehensively considering the appearance of the product, particle size (1% concentration dispersion liquid), dispersity (1% concentration dispersion liquid), pH change, and change in the content of hydroxyproline.

[0073] Table 3 Changes in particle size, PDI, PH, and content of hydroxyproline of milipin under different storage conditions

[0074]

[0075] From Figure 2 and Table 3, it can be seen that for milipin in Example 1, there were almost no changes in pH and the content of hydroxyproline after storage at different temperatures for 30 days. After storage at 45 °C for 30 days, the changes in the average particle size and PDI were relatively small, and the flexible liposomes of milipin were basically stable.

[0076] Example 7

[0077] Transdermal permeability test data

[0078] Transdermal penetration experiment of milipin

[0079] 1. Test method:

[0080] Experimental grouping and sample preparation: Control group: Essence prepared by directly adding hydroxyproline raw material to the base (hydroxyproline content is 2.5%); Treatment group: Essence prepared by adding milipin to the base (hydroxyproline content 2.5%); Essence base formula: SIMULGEL FL (copolymer of hydroxyethyl acrylate & sodium dimethyl taurate acrylate & isocetane & polysorbate 60) 0.8%, propylene glycol 3%, glycerol 3%, and the balance is water.

[0081] 2) Assembly and fixation of the test model: The skin simulation membrane (Strat-M) was fixed between the diffusion chamber and the receiving chamber of the vertical Franz diffusion cell. The rough side of the skin simulation membrane faced the diffusion chamber, and the smooth side faced the receiving chamber. After fixing the skin simulation membrane, 8 mL of receiving solution (7.4 PBS) was added to the receiving chamber to make the skin simulation membrane in close contact with the receiving solution.

[0082] 3) Fix the static vertical transdermal diffusion cell in the transdermal diffusion instrument, set the temperature to 32, and the rotation speed of the magnetic stirring rotor to 600 rpm.

[0083] 4) Sample loading: After the water bath temperature of the diffusion instrument is constant, perform sample loading. The sample addition amount is 0.1 g. Add the sample to the surface of the skin simulation membrane and spread it evenly.

[0084] 5) Collection of samples in the receiving cell: Take 300 μL of the receiving solution at 2, 5, 8, and 12 h respectively, and supplement fresh receiving solution to measure the concentration of hydroxyprolisane in the receiving solution to be tested.

[0085] 6) Perform quantitative analysis of hydroxyprolisane in the receiving solution by HPLC-ELSD.

[0086] 7) Data analysis: Use the following formula to quantitatively calculate the cumulative penetration amount of hydroxyprolisane in the receiving solution:

[0087]

[0088] In the formula:

[0089] Q: Cumulative penetration amount per unit area, referring to the penetration amount of the sample per unit area within the cumulative time;

[0090] Cn: Sample concentration at the nth sampling point; V: Volume of the receiving solution in the receiving cell;

[0091] V 0 : Volume of each sampling; A: Area of the receiving cell.

[0092] The results are as Figure 3 shown. After 5 h of penetration, the cumulative penetration amount of hydroxyprolisane in Mipuyin is higher than that of the control group. At 12 h of penetration, the cumulative penetration amount of the Mipuyin sample is about 2.6 times that of the control hydroxyprolisane penetration amount.

[0093] Example 8

[0094] Test on the moisturizing and anti-wrinkle effects of Mipuyin on the human body

[0095] Thirteen subjects aged 28 - 55 years were selected. The control group and the sample group were used on the left and right faces of the subjects respectively. They were applied twice a day during the normal morning and evening skin care process for 28 consecutive days. Skin physiological index tests were conducted before use, on the 14th day, and on the 28th day. Before the test, the face was cleaned uniformly and the facial moisture was dried. After sitting quietly for 30 minutes in an environment with constant temperature and humidity (21 ± 1°C, 50 ± 10%), skin index detection was carried out on the left and right faces respectively. Elasticity and firmness were tested with a skin elasticity tester (Cutometer®), transdermal water loss was tested with a skin surface water loss test probe (Tewameter®TM HEX), and the water content of the stratum corneum was tested with a skin moisture tester (Corneometer® CM825).

[0096] Composition of essence water matrix: 1.0% Carbopol 940, 0.5% p-hydroxyacetophenone, 3.0% 1,2 - hexanediol, 95.5% deionized water.

[0097] Control group: Mix 10 mL of 30% pro-xylane original liquid and 90 mL of essence water matrix.

[0098] Sample group: Mix 10 mL of milpuyin and 90 mL of essence water matrix.

[0099] Table 4 Human moisturizing and anti-wrinkle efficacy test of milpuyin and pro-xylane

[0100]

[0101] The test results are shown in Table 4. Both the control group and the sample group had obvious improvement effects on the 4 tested indicators. However, after 14 days of using the essence liquid of milpuyin in the sample group, the improvement effects on elasticity and firmness were more significant, and milpuyin had better anti-wrinkle and firming effects.

[0102] Example 9

[0103] Anti-wrinkle efficacy test of milpuyin

[0104] 1. Test method

[0105] 1) Tissue treatment: Immerse the freshly obtained skin tissue in 75% alcohol, wash for 30 s, and then wash three times with sterile PBS buffer; after that, cut the skin into tissue blocks of 24 ± 2 mm 2 with the epidermis facing up and the dermis facing down, place them in a culture mold, and then transfer the culture mold into a 6-well plate. Add 3.7 mL of culture medium to each well and culture in an incubator at 37°C and 5% CO 2 and change the liquid every day.

[0106] 2) Administration: After culturing the in vitro skin tissues for 2 days, irradiation and administration were started according to the test groups and corresponding treatment conditions in the table; the irradiation doses were UVA (30 J / cm 2 ) and UVB (50 mJ / cm 2 ), and continuous irradiation was performed for 4 days. After each irradiation, fresh culture medium was replaced, and administration treatment was carried out. PC (VC + VE) was administered by subcutaneous injection, and the test samples were administered by topical application. After continuous irradiation for 4 days, the in vitro skin tissues were cultured for another 3 days. During this period, no irradiation was performed, and only sample administration was carried out.

[0107] 3) Immunofluorescence test: The skin tissues used for detection were fixed with 4% paraformaldehyde. After 24 hours of fixation, immunofluorescence detection was carried out, and photographs were taken and observed under a fluorescence microscope, and the images were collected and analyzed.

[0108] 4) Calculation of promotion rate: Promotion rate = (sample group - negative control group) / negative control group × 100%.

[0109] 5) Statistical analysis of results: Graphs were plotted using graphing software (GraphPad Prism), and the results were expressed as mean ± standard deviation. The t-test was used for statistical analysis of comparisons between groups. All statistical analyses were two-tailed. P < 0.05 was considered to have a significant difference, and P < 0.01 was considered to have a highly significant difference.

[0110] Table 5 Test groups

[0111]

[0112] The experimental results are as Figures 4 to 7 shown in and Table 5. Compared with the BC group, the content of active collagen type I in the NC group decreased significantly, indicating that the stimulation conditions of this test were effective. Compared with the NC group, the content of active collagen type I in the PC group increased significantly, indicating that the positive control of this test was effective. Compared with the NC group, the content of active collagen type I in the samples Mipuyin - 2% and Mipuyin - 10% both increased significantly, and the promotion rates were 80.65% and 283.87% respectively. Compared with the BC group, the content of active collagen type IV in the NC group decreased significantly, indicating that the stimulation conditions of this test were effective. Compared with the NC group, the content of active collagen type IV in the PC group increased significantly, indicating that the positive control of this test was effective. Compared with the NC group, the content of active collagen type IV in the samples Mipuyin - 2% and Mipuyin - 10% both increased significantly, and the promotion rates were 109.38% and 134.38% respectively.

Claims

1. Application of glycoside derivative flexible liposomes in the preparation of moisturizing and anti-wrinkle products, characterized in that: The mass percentage of the glycoside derivative flexible liposome in the personal care product is 1-10%; the glycoside derivative flexible liposome is composed of the following components in mass parts: 2-8 parts of main shell material, 6-14 parts of moisturizer, 25-35 parts of wrapped raw material, 0.3-3 parts of auxiliary shell material, 1-2 parts of preservative and 38-65.7 parts of solvent; the main shell material is phosphatidylcholine; the auxiliary shell material is polyquaternium-51; the preservative is any one or more of 1,2-hexanediol, p-hydroxyacetophenone, methylparaben, phenoxyethanol and caprylyl glycol; the moisturizer is butylene glycol; the wrapped raw material is hydroxypropyl tetrahydrofurantriol; the solvent is deionized water; The preparation method comprises the following steps: heating the main shell material and the moisturizing agent in a water bath to 45-75°C and stirring evenly; then adding the coated raw material preheated to the same temperature and stirring evenly to obtain a mixture A; preheating the auxiliary shell material and the preservative to the same temperature, then adding them into the mixture A and continuously stirring to obtain a mixture B, and cooling the mixture to 35-45°C; injecting the solvent preheated to 35-45°C into the mixture B, continuously stirring at a speed of 600-800rpm, and continuously hydrating for 15-25min after the injection is completed to obtain colostrum; homogenizing the colostrum with a high-pressure homogenizer, setting the homogenization pressure to 12000-15000psi, and the number of homogenization cycles to 3 times to obtain a glycoside derivative flexible liposome, wherein the particle size of the glycoside derivative flexible liposome is 10-40nm; controlling the discharge temperature of the first homogenization to 20-30°C, the discharge temperature of the second homogenization to 15-25°C, and the discharge temperature of the third homogenization to 10-20°C.

2. The use according to claim 1, characterized in that: The discharge temperature of the first homogenization pass was controlled at 25°C, the discharge temperature of the second homogenization pass was controlled at 20°C, and the discharge temperature of the third homogenization pass was controlled at 15°C.

3. The use according to claim 1, characterized in that: The colostrum was homogenized using a high pressure homogenizer, the homogenization pressure was set to 14000 psi, and the number of homogenization cycles was 3 times.

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