Extraction method and application of sarcodia montagneana extract capable of inhibiting bacteria, removing dandruff, controlling oil and relieving

Abstract

The invention relates to an extraction method and application of a sarcodia montagneana extract for inhibiting bacteria, removing dandruff, controlling oil and relieving, and belongs to the technical field of sarcodia montagneana extracts. The extraction method of the sarcodia montagneana extract comprises the following steps: cleaning fresh sarcodia montagneana with deionized water, soaking the cleaned sarcodia montagneana in a compound solution, grinding the soaked sarcodia montagneana to obtain slurry, freeze-drying the slurry, putting the freeze-dried slurry into a supercritical fluid extractor, adjusting corresponding temperature and pressure, and then extracting the freeze-dried sarcodia montagneana by using a supercritical fluid extractor; and fully contacting the supercritical fluid with the sarcodia montagneana powder, performing extraction treatment, and adjusting pressure through a pressure reducer for separation to obtain the sarcodia montagneana extract. The sarcodia montagneana extract contains galactose, fucose, mannose and glucuronic acid, has a remarkable removal effect on ROS and DPPH free radicals, has high antioxidant activity, can serve as a natural antioxidant and has good effects of inhibiting bacteria, removing dandruff, controlling oil and relieving.

Description

Technical Field

[0001] The invention belongs to the technical field of sea auricularia auricula extracts and relates to an extraction method and application of sea auricularia auricula extracts with antibacterial, anti-dandruff, oil-control and soothing effects. Background Art

[0002] Sea fungus, belonging to the genus Auricularia under the Rhodophyta, is a special algae plant that mainly grows in the depths of the ocean. This plant not only has all the nutrients that ordinary fungus has, but is also rich in a variety of trace elements necessary for the human body, such as iodine, calcium, iron, etc. In addition, sea fungus is also rich in amino acids and vitamins, which are of great benefit to human health.

[0003] Sea fungus not only contains all the nutrients contained in edible plants on land, such as protein, vitamins, cellulose, and various trace elements such as calcium and iron, but also contains more than 20 nutrients unique to the ocean. These unique nutrients include alginate, alginic acid, fucoxanthin, and EPA (an unsaturated fatty acid). Due to its rich nutritional value, sea fungus is known as the "longevity vegetable" in Japan, and in many countries, it is honored as the "first of marine vegetables." This praise not only reflects the unique nutritional advantages of sea fungus, but also reflects its important position in a healthy diet.

[0004] Sea fungus extract has a strong antioxidant effect and is a free radical scavenger. It can effectively reduce the invasion of harmful substances and maintain skin health. Sea fungus extract can reduce inflammatory reactions, make the skin smoother and softer, and improve problems such as dryness, roughness and sensitivity. It is suitable for sensitive skin. Summary of the invention

[0005] The purpose of the present invention is to provide an extraction method and application of a sea fungus extract with antibacterial, anti-dandruff, oil control and soothing effects. The sea fungus extract used in the present invention contains galactose, fucose, mannose and glucuronic acid, has a significant scavenging effect on ROS and DPPH free radicals, and has strong antioxidant activity. It can be used as a natural antioxidant and has good antibacterial, anti-dandruff, oil control and soothing effects.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0008] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0009] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0010] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0011] As a preferred technical solution of the present invention, in step 1), the first step of freeze drying refers to freezing at -20°C to -40°C for 1-2h.

[0012] As a preferred technical solution of the present invention, in step 1), the second step of freeze drying refers to freezing at -30°C to -50°C for 6-8h.

[0013] As a preferred technical solution of the present invention, in step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.4-2.8:1-3:50; the usage ratio of sea fungus to the compound liquid is 1g:10mL.

[0014] As a preferred technical solution of the present invention, in step 1), the soaking treatment is soaking at 50-60°C for 35-45 minutes.

[0015] As a preferred technical solution of the present invention, in step 1), the grinding time is 1-3 minutes.

[0016] As a preferred technical solution of the present invention, in step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:55-80.

[0017] As a preferred technical solution of the present invention, in step 2), the temperature is 50-60°C, the pressure is 1.2-2Mpa, and the extraction time is 3-4h.

[0018] As a preferred technical solution of the present invention, in step 3), the rotation speed and time of the centrifugation are 2000-4000 rpm and 10-20 min, respectively.

[0019] As a preferred technical solution of the present invention, in step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 3-8 kDa, and the third step of freeze-drying refers to freezing at -30°C to -50°C for 12-15 hours.

[0020] Beneficial effects of the present invention:

[0021] (1) The Auricularia auricula extract used in the present invention contains galactose, fucose, mannose and glucuronic acid, which has a significant scavenging effect on ROS and DPPH free radicals, and has strong antioxidant activity. It can be used as a natural antioxidant and has good antibacterial, anti-dandruff, oil control and soothing effects;

[0022] (2) Freeze-drying the sea fungus after washing can promote the dissolution of active substances in the subsequent extraction process and improve the extraction efficiency;

[0023] (3) Before extraction, the mixture is first soaked in a compound solution. The nanocellulose and acetic acid in the compound solution can synergistically improve the extraction of effective ingredients in Auricularia auricula. DETAILED DESCRIPTION

[0024] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0025] Example 1

[0026] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0027] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0028] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0029] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0030] In step 1), the first step of freeze drying refers to freezing at -20°C for 2 hours.

[0031] In step 1), the second step of freeze drying refers to freezing at -35°C for 8 hours.

[0032] In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.5:2:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0033] In step 1), the soaking treatment is soaking at 52° C. for 39 minutes.

[0034] In step 1), the grinding time is 2 minutes.

[0035] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:62.

[0036] In step 2), the temperature is 55° C., the pressure is 1.5 MPa, and the extraction time is 3.5 h.

[0037] In step 3), the centrifugal speed and time are 3000 rpm and 12 min respectively.

[0038] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 4 kDa, and the third step of freeze drying refers to freezing at -35°C for 13 hours.

[0039] Example 2

[0040] A method for extracting auricularia auricula extract comprises the following steps:

[0041] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0042] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0043] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0044] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0045] In step 1), the first step of freeze drying refers to freezing at -32°C for 1.5 hours.

[0046] In step 1), the second step of freeze drying refers to freezing at -40°C for 6.5 hours.

[0047] In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.7:2.2:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0048] In step 1), the soaking treatment is soaking at 55° C. for 36 minutes.

[0049] In step 1), the grinding time is 1.5 min.

[0050] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:70.

[0051] In step 2), the temperature is 58° C., the pressure is 1.3 MPa, and the extraction time is 4 hours.

[0052] In step 3), the centrifugal speed and time are 2500 rpm and 16 min respectively.

[0053] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 5 kDa, and the third step of freeze drying refers to freezing at -38°C for 12.5 hours.

[0054] Example 3

[0055] A method for extracting auricularia auricula extract comprises the following steps:

[0056] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0057] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0058] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0059] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0060] In step 1), the first step of freeze drying refers to freezing at -38°C for 1.1 h.

[0061] In step 1), the second step of freeze drying refers to freezing at -45°C for 6.2 hours.

[0062] In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.6:2.3:50; the dosage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0063] In step 1), the soaking treatment is soaking at 56° C. for 42 minutes.

[0064] In step 1), the grinding time is 3 minutes.

[0065] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:72.

[0066] In step 2), the temperature is 55° C., the pressure is 2 MPa, and the extraction time is 3 h.

[0067] In step 3), the centrifugal speed and time are 4000 rpm and 12 min respectively.

[0068] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 6 kDa, and the third step of freeze drying refers to freezing at -42°C for 13 hours.

[0069] Example 4

[0070] A method for extracting auricularia auricula extract comprises the following steps:

[0071] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0072] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0073] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0074] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0075] In step 1), the first step of freeze drying refers to freezing at -28°C for 2 hours.

[0076] In step 1), the second step of freeze drying refers to freezing at -38°C for 7.5 hours.

[0077] In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.8:1.7:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0078] In step 1), the soaking treatment is soaking at 54° C. for 43 minutes.

[0079] In step 1), the grinding time is 2.8 min.

[0080] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:69.

[0081] In step 2), the temperature is 57° C., the pressure is 1.8 MPa, and the extraction time is 4 hours.

[0082] In step 3), the centrifugal speed and time are 2000 rpm and 20 min respectively.

[0083] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 4 kDa, and the third step of freeze drying refers to freezing at -39°C for 14 hours.

[0084] Example 5

[0085] A method for extracting auricularia auricula extract comprises the following steps:

[0086] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0087] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0088] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0089] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0090] In step 1), the first step of freeze drying refers to freezing at -36°C for 1.7 hours.

[0091] In step 1), the second step of freeze drying refers to freezing at -46°C for 6.5 hours.

[0092] In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.5:2.1:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0093] In step 1), the soaking treatment is soaking at 60° C. for 35 minutes.

[0094] In step 1), the grinding time is 1.3 min.

[0095] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:59.

[0096] In step 2), the temperature is 60° C., the pressure is 1.2 MPa, and the extraction time is 3.6 h.

[0097] In step 3), the centrifugal speed and time are 3000 rpm and 18 min, respectively.

[0098] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 8 kDa, and the third step of freeze drying refers to freezing at -45°C for 12.2 hours.

[0099] Comparative Example 1

[0100] Compared with Example 5, the difference in Comparative Example 1 is that the sea fungus is not freeze-dried after washing, but is directly soaked, that is:

[0101] A method for extracting auricularia auricula extract comprises the following steps:

[0102] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0103] 1) washing fresh sea fungus with deionized water, then soaking it in a compound solution, then grinding it to obtain a slurry, and performing a second step of freeze drying to obtain sea fungus powder;

[0104] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0105] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0106] In step 1), the first step of freeze drying refers to freezing at -36°C for 1.7 hours.

[0107] In step 1), the second step of freeze drying refers to freezing at -46°C for 6.5 hours.

[0108] In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.5:2.1:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0109] In step 1), the soaking treatment is soaking at 60° C. for 35 minutes.

[0110] In step 1), the grinding time is 1.3 min.

[0111] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:59.

[0112] In step 2), the temperature is 60° C., the pressure is 1.2 MPa, and the extraction time is 3.6 h.

[0113] In step 3), the centrifugal speed and time are 3000 rpm and 18 min, respectively.

[0114] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 8 kDa, and the third step of freeze drying refers to freezing at -45°C for 12.2 hours.

[0115] Comparative Example 2

[0116] Compared with Example 5, the difference in Comparative Example 2 is that the composite liquid does not contain nanocellulose, that is:

[0117] A method for extracting auricularia auricula extract comprises the following steps:

[0118] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0119] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0120] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0121] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0122] In step 1), the first step of freeze drying refers to freezing at -36°C for 1.7 hours.

[0123] In step 1), the second step of freeze drying refers to freezing at -46°C for 6.5 hours.

[0124] In step 1), the compound liquid is composed of glacial acetic acid and deionized water in a mass ratio of 4.6:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0125] In step 1), the soaking treatment is soaking at 60° C. for 35 minutes.

[0126] In step 1), the grinding time is 1.3 min.

[0127] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:59.

[0128] In step 2), the temperature is 60° C., the pressure is 1.2 MPa, and the extraction time is 3.6 h.

[0129] In step 3), the centrifugal speed and time are 3000 rpm and 18 min, respectively.

[0130] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 8 kDa, and the third step of freeze drying refers to freezing at -45°C for 12.2 hours.

[0131] Comparative Example 3

[0132] Compared with Example 2, the difference in Comparative Example 3 is that the compounded liquid does not contain glacial acetic acid, that is:

[0133] A method for extracting auricularia auricula extract comprises the following steps:

[0134] A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, the method comprising the following steps:

[0135] 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder;

[0136] 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract;

[0137] 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane and then freeze-dried to obtain the Auricularia auricula extract.

[0138] In step 1), the first step of freeze drying refers to freezing at -36°C for 1.7 hours.

[0139] In step 1), the second step of freeze drying refers to freezing at -46°C for 6.5 hours.

[0140] In step 1), the compound liquid is composed of nanocellulose and deionized water in a mass ratio of 4.6:50; the usage ratio of the sea fungus to the compound liquid is 1 g:10 mL.

[0141] In step 1), the soaking treatment is soaking at 60° C. for 35 minutes.

[0142] In step 1), the grinding time is 1.3 min.

[0143] In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:59.

[0144] In step 2), the temperature is 60° C., the pressure is 1.2 MPa, and the extraction time is 3.6 h.

[0145] In step 3), the centrifugal speed and time are 3000 rpm and 18 min, respectively.

[0146] In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 8 kDa, and the third step of freeze drying refers to freezing at -45°C for 12.2 hours.

[0147] The samples prepared in Examples 1-5 and Comparative Examples 1-3 were respectively tested for free radical elimination performance, and the test results are shown in Table 1.

[0148] Free radical elimination performance test:

[0149] The sample solutions of Examples 1-5 and Comparative Examples 1-3 were prepared with serum-free DMEM culture medium at a concentration of 0.5%, and sterilized by filtration with a 0.22 μm filter membrane. Then, the dichlorofluorescein diacetate (DCFH-DA) probe solution was prepared by diluting 0.375 uLDCFH-DA to 1 mL PBS. HaCaT cells in the logarithmic growth phase were taken and cultured at 1×10 5 Cells were inoculated at a density of 100 cells / mL in a 24-well culture plate, and 12 wells were selected. Each well contained 2 mL of cell suspension and was placed in a carbon dioxide incubator at 37°C and 5% CO2 for 24 hours. 2 The intensity of irradiation was 1h, and UVB was 700μW / cm 2 The cells were irradiated with an intensity of 100 μg / cm2 for 3 min, and the normal control group was not irradiated. After irradiation, the old culture medium was discarded, and the sample solution was added to the experimental group, and serum-free DMEM culture medium was added to the model group and the normal control group, with 2 mL in each well. After continuing to culture for 16 h, all the culture medium was discarded and washed twice with PBS. 1.5 mL of DCFH-DA solution was added to each well, and the cells were placed in a 37°C cell culture incubator for another 30 min. The cells were mixed every 5 min to allow the probe to fully bind. The probe was discarded, and the cells were washed twice with preheated serum-free culture medium. 1 mL of serum-free culture medium was added to each well and incubated at 37°C for 10 min. After washing once with PBS, the cells were digested with trypsin, washed twice with PBS, resuspended in 300 μL PBS, and detected by dual-channel flow cytometer (the cells were filtered with a 100-mesh filter before being put on the machine), FL1-H channel, and 10,000 cells were collected for each sample;

[0150] Clearance rate = (model group - experimental group) / model group × 100%;

[0151] Table 1

[0152]

[0153] From the test results in Table 1, it can be seen that compared with Comparative Examples 1-3, the compositions prepared in Examples 1-5 have excellent antioxidant properties. The sea fungus extract used in the present invention contains galactose, fucose, mannose and glucuronic acid, has a significant scavenging effect on ROS and DPPH free radicals, and has strong antioxidant activity. The sea fungus extract is rich in natural antioxidant substances such as polysaccharides. Before extraction, the sea fungus is first freeze-dried to promote the rupture of cell membranes and promote the efficiency of subsequent extraction. During the extraction process, the added nanocellulose contains a large amount of hydroxyl groups, which can effectively promote the extraction of effective ingredients in the sea fungus in coordination with glacial acetic acid and improve the antioxidant effect.

[0154] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any indirect modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties, characterized in that: The extraction method comprises the following steps: 1) washing the fresh sea fungus with deionized water, performing a first freeze-drying step, then placing it in a composite liquid for soaking, then grinding it to obtain a slurry, and performing a second freeze-drying step to obtain sea fungus powder; 2) adding the Auricularia auricula powder to deionized water, adjusting the corresponding temperature and pressure, and performing high-pressure extraction to obtain an extract; 3) After the extract is centrifuged, the supernatant is filtered through an ultrafiltration membrane, and then freeze-dried in the third step to obtain the Auricularia auricula extract.

2. The method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties according to claim 1, characterized in that: In step 1), the first step of freeze drying refers to freezing at -20°C to -40°C for 1-2 hours.

3. The method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties according to claim 1, characterized in that: In step 1), the second step of freeze drying refers to freezing at -30°C to -50°C for 6-8h.

4. The method for extracting a fungus extract with antibacterial, anti-dandruff, oil-control and soothing properties according to claim 1, characterized in that: In step 1), the compound liquid is composed of nanocellulose, glacial acetic acid and deionized water in a mass ratio of 2.4-2.8:1-3:50; the dosage ratio of the sea fungus to the compound liquid is 1g:10mL.

5. The method for extracting the Auricularia auricula extract with antibacterial, anti-dandruff, oil-control and soothing effects according to claim 1, characterized in that: In step 1), the soaking treatment is soaking at 50-60° C. for 35-45 minutes.

6. The method for extracting the Auricularia auricula extract with antibacterial, anti-dandruff, oil-control and soothing effects according to claim 1, characterized in that: In step 1), the grinding time is 1-3 min.

7. The method for extracting the Auricularia auricula extract with antibacterial, anti-dandruff, oil-control and soothing effects according to claim 1, characterized in that: In step 2), the mass ratio of the Auricularia auricula powder to deionized water is 1:55-80.

8. The method for extracting the Auricularia auricula extract with antibacterial, anti-dandruff, oil-control and soothing effects according to claim 1, characterized in that: In step 2), the temperature is 50-60° C., the pressure is 1.2-2 MPa, and the extraction time is 3-4 h.

9. The method for extracting the Auricularia auricula extract with antibacterial, anti-dandruff, oil-control and soothing effects according to claim 1, characterized in that: In step 3), the centrifugal speed and time are 2000-4000 rpm and 10-20 min, respectively.

10. The method for extracting the Auricularia auricula extract with antibacterial, anti-dandruff, oil-control and soothing effects according to claim 1, characterized in that: In step 3), the ultrafiltration membrane refers to an ultrafiltration membrane with a molecular weight of 3-8 kDa, and the third step of freeze drying refers to freezing at -30°C to -50°C for 12-15 hours.

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