A python oil composition with skin repair function after sunburn and preparation method thereof

Abstract

The invention provides a python oil composition with a skin repairing function after sunburn and a preparation method thereof. The raw materials include python oil extract, allantoin, glycerol, carboxymethyl chitosan, sodium hyaluronate and frankincense resin extract. The cumulative permeability of the python oil composition of the invention within 6 hours is as high as 83%, the DPPH free radical scavenging rate is 90%, and the hydroxyl free radical scavenging rate is 87%, indicating that the python oil composition has a good permeability effect and is conducive to quickly repairing the skin state after sunburn.

Description

Technical Field

[0001] The invention relates to the technical field of skin after-sunburn repair compositions, and in particular to a python oil composition with a skin after-sunburn repair function and a preparation method thereof. Background Art

[0002] In recent years, due to the increasing ultraviolet (UV) radiation, the incidence of various skin diseases such as sunburn, tanning, polymorphous light eruption and even skin acne caused by UV light damage to the skin has gradually increased. The UV in sunlight can be divided into three bands: short-wave ultraviolet (UVC), medium-wave ultraviolet (UVB) and long-wave ultraviolet (UVA), with wavelengths of 200-280, 280-320 and 320-400nm respectively. Among them, ultraviolet rays with a wavelength of 290-400nm can cause skin damage, and the number of skin red spots caused by UV increases in a dose-dependent manner. UV irradiation can induce the production of reactive oxygen free radicals (ROS), destroy the activity of antioxidant enzymes in cells, destroy biofilms and cause keratinocyte apoptosis.

[0003] Python fat, namely python oil, is rich in unsaturated fatty acids such as linoleic acid and linolenic acid, which is more than 60% and has good permeability. Studies have shown that the amount of unsaturated fatty acids in python fat is relatively high, and the efficacy of python fat is related to the unsaturated fatty acids it contains. It has the effects of regulating endocrine disorders, preventing skin aging, preventing vascular sclerosis, reducing swelling, inhibiting bacterial growth, and promoting blood circulation. Pure python oil has strong penetration ability and can quickly repair the skin, but the cost of using pure python oil for skin sunburn is relatively high. Therefore, a python oil composition with low cost, good penetration effect, and good skin repair effect after sunburn is proposed. Summary of the invention

[0004] In view of this, the present invention proposes a python oil composition with the function of repairing skin after sunburn and a preparation method thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A python oil composition with the function of repairing skin after sunburn comprises, by weight percentage, 8-12% python oil extract, 0.2-0.5% allantoin, 25-30% glycerol, 2-4% carboxymethyl deacetylated chitosan, 0.05-0.3% sodium hyaluronate and the remainder water.

[0007] Furthermore, the composition further comprises 0.04-0.08% of Boswellia serrata resin extract by weight percentage.

[0008] Furthermore, the preparation method of the python oil extract comprises the following steps:

[0009] (1) freeze-drying snake fat, and crushing the frozen snake fat to obtain fat powder;

[0010] (2) extracting the fat powder using an extraction kettle at an extraction temperature of 35-45° C., an extraction pressure of 0.3-1.0 MPa, and an extraction time of 120-150 min to obtain a crude snake oil;

[0011] (3) adding the primary crude snake oil into a decomposition kettle at a decomposition temperature of 90-100° C. and a decomposition pressure of 0.2 MPa-0.4 MPa to obtain secondary crude snake oil;

[0012] (4) adding a mixed enzyme to the secondary crude snake oil for enzymolysis to obtain enzymolyzed snake oil;

[0013] (5) The snake oil after enzymatic hydrolysis is filtered using a macroporous resin to obtain a python oil extract.

[0014] Furthermore, in step (1), the freeze-drying time is 10-12 hours, and the freezing temperature is -45 to -50°C.

[0015] Furthermore, in step (2), the extractant is dichloromethane, and the dichloromethane flow rate is 4-6 kg / hr.

[0016] Furthermore, in step (4), the mixed enzyme comprises trypsin and Rhizomucor miehei lipase in a mass ratio of 2-5:1.

[0017] Furthermore, in step (4), the mass ratio of the mixed enzyme to the secondary crude snake oil is 0.03-0.05:10.

[0018] Furthermore, in step (5), the macroporous resin is filtered by eluting with 5-8 BV of an ethanol solution with a concentration of 50-70 wt%, and then eluting with 10-15 BV of an ethanol solution with a concentration of 30-40 wt%.

[0019] A method for preparing a python oil composition having a skin repairing function after sunburn, comprising the following steps:

[0020] S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A;

[0021] S2: Add water to component A and stir at a stirring temperature of 70-80°C, stirring at 50-60 r / min for 15-25 min, and stirring again at 20-40 r / min for 10-15 min to obtain component B;

[0022] S3: Add python oil extract and frankincense resin extract to component B, stir at 15-25 r / min for 3-5 min at 50-60° C., and keep warm for 20-30 min to obtain a finished product.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention uses python oil extract, allantoin, glycerol, carboxymethyl chitosan, and sodium hyaluronate to prepare a python oil composition with a skin sunburn repair function. The python oil adopts a specific extraction method to extract the effective components of the python oil for repairing sunburn, which is conducive to adding a small amount of python oil in the composition to achieve a good modification effect. The present invention also contains a serrata frankincense resin extract, which can improve the anti-inflammatory effect of the python oil composition, effectively resist skin cell apoptosis caused by UV irradiation, and improve the repair effect of the python composition. The cumulative penetration rate of the 6h python oil composition of the present invention is as high as 83%, indicating that the python oil composition has a good penetration effect and is conducive to quickly repairing the skin state after skin sunburn. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0026] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0027] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0028] Boswellia serrata resin extract was purchased from Shaanxi Denis Biotechnology Co., Ltd.

[0029] The trypsin enzyme activity was 60 U / mL and the Rhizomucor miehei lipase enzyme activity was 80 U / mL.

[0030] Example 1

[0031] Python oil extract is prepared by the following steps:

[0032] (1) Freezing python fat at -50°C for 10 h and crushing it to obtain fat powder;

[0033] (2) extracting the fat powder in an extraction kettle at an extraction temperature of 35° C., an extraction pressure of 0.3 MPa, an extraction time of 120 min, an extractant of dichloromethane, and a dichloromethane flow rate of 4 kg / hr to obtain a crude snake oil;

[0034] (3) adding the primary crude snake oil into a decomposition kettle at a decomposition temperature of 90° C. and a decomposition pressure of 0.2 MPa to obtain secondary crude snake oil;

[0035] (4) adding trypsin and Rhizomucor miehei lipase in a mass ratio of 2:1 to the secondary crude snake oil for enzymolysis, the mass ratio of the mixed enzyme to the secondary crude snake oil is 0.03:10, the enzymolysis temperature is 40° C., the enzymolysis time is 3 h, and after the enzymolysis is completed, heating is performed at 105° C. for 1 min to obtain the enzymolyzed snake oil;

[0036] (5) The snake oil after enzymatic hydrolysis was filtered using HPD-400 macroporous resin, eluted using 5 BV of 50 wt % ethanol solution, and then eluted using 10 BV of 30 wt % ethanol solution to obtain the python oil extract.

[0037] The composition comprises 8% python oil extract, 0.2% allantoin, 25% glycerin, 2% carboxymethyl chitosan, 0.05% sodium hyaluronate, 0.4% frankincense resin extract and the balance water according to weight percentage.

[0038] The python oil composition is prepared by the following steps:

[0039] S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A;

[0040] S2: Add water to component A and stir at 70°C, stirring at 50 r / min for 15-25 min, and stirring again at 20 r / min for 10 min to obtain component B;

[0041] S3: Add python oil extract and frankincense resin extract to component B, stir at 15 r / min for 3 min at 50° C., and keep warm for 20 min to obtain a finished product.

[0042] Example 2

[0043] Python oil extract is prepared by the following steps:

[0044] (1) Freezing python fat at -50°C for 10 hours and crushing it to obtain fat powder;

[0045] (2) extracting the fat powder in an extraction kettle at an extraction temperature of 45° C., an extraction pressure of 1.0 MPa, an extraction time of 150 min, an extractant of dichloromethane, and a dichloromethane flow rate of 6 kg / hr to obtain a crude snake oil;

[0046] (3) adding the primary crude snake oil into a decomposition kettle at a decomposition temperature of 100° C. and a decomposition pressure of 0.4 MPa to obtain secondary crude snake oil;

[0047] (4) adding trypsin and Rhizomucor miehei lipase in a mass ratio of 5:1 to the secondary crude snake oil for enzymolysis, the mass ratio of the mixed enzyme to the secondary crude snake oil is 0.05:10, the enzymolysis temperature is 50° C., the enzymolysis time is 5 h, and after the enzymolysis is completed, heating is performed at 105° C. for 1 min to obtain the enzymolyzed snake oil;

[0048] (5) The enzymatically hydrolyzed snake oil was filtered using HPD-400 macroporous resin, eluted using 8 BV of 70 wt % ethanol solution, and then eluted using 15 BV of 40 wt % ethanol solution to obtain the python oil extract.

[0049] The composition comprises 12% python oil extract, 0.5% allantoin, 30% glycerin, 4% carboxymethyl chitosan, 0.3% sodium hyaluronate, 0.8% frankincense resin extract and the balance water according to weight percentage.

[0050] The python oil composition is prepared by the following steps:

[0051] S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A;

[0052] S2: Add water to component A and stir at 80°C, stirring at 60 r / min for 25 min, and stirring again at 40 r / min for 15 min to obtain component B;

[0053] S3: Add python oil extract and frankincense resin extract to component B, stir at 25 r / min for 5 min at 60° C., and keep warm for 30 min to obtain a finished product.

[0054] Example 3

[0055] Python oil extract is prepared by the following steps:

[0056] (1) Freezing python fat at -50°C for 10 h and crushing it to obtain fat powder;

[0057] (2) extracting the fat powder in an extraction kettle at an extraction temperature of 40° C., an extraction pressure of 0.6 MPa, an extraction time of 140 min, an extractant of dichloromethane, and a dichloromethane flow rate of 5 kg / hr to obtain a crude snake oil;

[0058] (3) adding the primary crude snake oil into a decomposition kettle at a decomposition temperature of 95° C. and a decomposition pressure of 0.3 MPa to obtain secondary crude snake oil;

[0059] (4) adding trypsin and Rhizomucor miehei lipase in a mass ratio of 3.5:1 to the secondary crude snake oil for enzymolysis, the mass ratio of the mixed enzyme to the secondary crude snake oil is 0.04:10, the enzymolysis temperature is 5° C., the enzymolysis time is 4 h, and after the enzymolysis is completed, heating is performed at 105° C. for 1 min to obtain the enzymolyzed snake oil;

[0060] (5) The enzymatically hydrolyzed snake oil was filtered using HPD-400 macroporous resin, eluted using 6 BV of 60 wt % ethanol solution, and then eluted using 13 BV of 35 wt % ethanol solution to obtain a python oil extract.

[0061] The composition comprises 10% python oil extract, 0.35% allantoin, 28% glycerin, 3% carboxymethyl chitosan, 0.2% sodium hyaluronate, 0.6% frankincense resin extract and the balance water according to weight percentage.

[0062] The python oil composition is prepared by the following steps:

[0063] S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A;

[0064] S2: Add water to component A and stir at a stirring temperature of 75°C, stirring at 55 r / min for 20 min, and stirring again at 30 r / min for 10 min to obtain component B;

[0065] S3: Add python oil extract and frankincense resin extract to component B, stir at 20 r / min for 4 min at 55° C., and keep warm for 25 min to obtain a finished product.

[0066] Example 4

[0067] Python oil extract is prepared by the following steps:

[0068] (1) Freezing python fat at -50°C for 10 h and crushing it to obtain fat powder;

[0069] (2) extracting the fat powder in an extraction kettle at an extraction temperature of 40° C., an extraction pressure of 0.6 MPa, an extraction time of 140 min, an extractant of dichloromethane, and a dichloromethane flow rate of 5 kg / hr to obtain a crude snake oil;

[0070] (3) adding the primary crude snake oil into a decomposition kettle at a decomposition temperature of 95° C. and a decomposition pressure of 0.3 MPa to obtain secondary crude snake oil;

[0071] (4) adding trypsin and Rhizomucor miehei lipase in a mass ratio of 3.5:1 to the secondary crude snake oil for enzymolysis, the mass ratio of the mixed enzyme to the secondary crude snake oil is 0.04:10, the enzymolysis temperature is 5° C., the enzymolysis time is 4 h, and after the enzymolysis is completed, heating is performed at 105° C. for 1 min to obtain the enzymolyzed snake oil;

[0072] (5) The enzymatically hydrolyzed snake oil was filtered using HPD-400 macroporous resin, eluted using 6 BV of 60 wt % ethanol solution, and then eluted using 13 BV of 35 wt % ethanol solution to obtain a python oil extract.

[0073] The composition includes 10% python oil extract, 0.35% allantoin, 28% glycerol, 3% carboxymethyl chitosan, 0.2% sodium hyaluronate and the balance water.

[0074] The python oil composition is prepared by the following steps:

[0075] S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A;

[0076] S2: Add water to component A and stir at a stirring temperature of 75°C, stirring at 55 r / min for 20 min, and stirring again at 30 r / min for 10 min to obtain component B;

[0077] S3: Add the python oil extract to component B, stir at 20 r / min for 4 min at 55° C., and keep warm for 25 min to obtain a finished product.

[0078] Comparative Example 1

[0079] The extraction method of python oil was adjusted based on Example 3, specifically:

[0080] Python oil extract is prepared by the following steps:

[0081] (1) Freezing python fat at -50°C for 10 h and crushing it to obtain fat powder;

[0082] (2) extracting the fat powder in an extraction kettle at an extraction temperature of 40° C., an extraction pressure of 0.6 MPa, an extraction time of 140 min, an extractant of dichloromethane, and a dichloromethane flow rate of 5 kg / hr to obtain a crude snake oil;

[0083] (3) Add the crude snake oil into a decomposition kettle at a decomposition temperature of 95° C. and a decomposition pressure of 0.3 MPa to obtain a snake oil extract.

[0084] The composition comprises 10% python oil extract, 0.35% allantoin, 28% glycerin, 3% carboxymethyl chitosan, 0.2% sodium hyaluronate, 0.6% frankincense resin extract and the balance water according to weight percentage.

[0085] The python oil composition is prepared by the following steps:

[0086] S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A;

[0087] S2: Add water to component A and stir at a stirring temperature of 75°C, stirring at 55 r / min for 20 min, and stirring again at 30 r / min for 10 min to obtain component B;

[0088] S3: Add python oil extract and frankincense resin extract to component B, stir at 20 r / min for 4 min at 55° C., and keep warm for 25 min to obtain a finished product.

[0089] Comparative Example 2

[0090] The raw materials were adjusted on the basis of Example 3, specifically:

[0091] The composition includes 10% python oil extract, 0.35% vitamin E, 28% propylene glycol, 3% PEG-40 hydrogenated castor oil, 0.2% sodium hyaluronate, 0.6% frankincense resin extract and the balance water, measured by weight percentage.

[0092] The python oil composition is prepared by the following steps:

[0093] S1: Mixing vitamin E, propylene glycol, PEG-40 hydrogenated castor oil and sodium hyaluronate to prepare component A;

[0094] S2: Add water to component A and stir at a stirring temperature of 75°C, stirring at 55 r / min for 20 min, and stirring again at 30 r / min for 10 min to obtain component B;

[0095] S3: Add python oil extract and frankincense resin extract to component B, stir at 20 r / min for 4 min at 55° C., and keep warm for 25 min to obtain a finished product.

[0096] Test Example 1

[0097] The python oil compositions prepared in Examples 3-4 and Comparative Examples 1-2 were used to carry out DPPH free radical scavenging tests and hydroxyl free radical scavenging tests.

[0098] DPPH free radical scavenging test: add 3 ml of 0.2 mmol / L DPPH anhydrous ethanol solution into a cuvette, then add 1 ml of the python oil composition and mix well, react at room temperature in the dark for 30 minutes, and measure the absorbance at 517 nm; use 3 ml of anhydrous ethanol and 1 ml of the sample to be tested as blank controls, and measure the absorbance; use 3 ml of DPPH anhydrous ethanol solution and 1 ml of anhydrous ethanol solution as control groups, and measure the absorbance, recorded as A, and calculate the DPPH free radical scavenging rate according to the formula.

[0099] DPPH free radical scavenging rate = 1-(experimental group-blank control) / control group × 100%

[0100] In a test tube, add 0.5 ml of 0.75 mmol / L o-phenanthroline anhydrous ethanol solution, then add 2 ml of 0.2 mol / L pH 7.4 phosphate buffer and 1 ml of python oil composition, mix thoroughly, add 0.5 ml of 0.75 mmol / L ferric sulfate heptahydrate solution, mix again, add 0.5 ml of 0.01% hydrogen peroxide solution, and place in a 37°C water bath for 1 hour, measure the absorbance at 536 m, and calculate the hydroxyl radical scavenging rate according to the formula.

[0101] Hydroxyl free radical scavenging rate = (experimental group - blank group) / (control group - blank group) × 100%

[0102] name DPPH free radical scavenging rate (%) Hydroxyl free radical scavenging rate (%) Example 3 90.49 87.68 Example 4 85.61 83.41 Comparative Example 1 81.63 79.15 Comparative Example 2 84.88 81.67

[0103] The experimental results show that the python oil composition prepared by the present invention has a good scavenging effect on DPPH free radical scavenging and hydroxyl free radical scavenging. The experimental results of Example 4 and Example 3 show that the addition of the serrata frankincense resin extract in the present invention can improve the use effect of the python oil composition. The experimental results of Comparative Example 1 show that the preparation method of the python oil of the present invention can further extract the effective ingredients in the python oil, thereby achieving the purpose of reducing costs and improving the use effect. The experimental results of Comparative Example 2 show that the auxiliary materials of the present invention and the python oil and serrata frankincense resin extract are reasonably compounded to improve the use effect of the composition.

[0104] Test Example 2 In vitro skin permeation test

[0105] The experiment was conducted with reference to the in vitro transdermal behavior investigation method in "Preparation of Quercus-Astragalus Whitening Sunscreen Gel and Investigation of In Vitro Transdermal Behavior", in which the present invention adopted the UV method to measure the absorbance at 230 nm and calculate the content. The dosage of the present invention was 1.0 g, and the cumulative permeability of 6 hours was calculated.

[0106] The control group used

[0107] name Example 3 Example 4 Comparative Example 1 Comparative Example 2 Cumulative penetration rate (%) 83.48 80.33 73.84 78.61

[0108] The test results show that the python oil composition of the present invention has a good penetration effect. The results of Example 4 show that the addition of the extract of Boswellia serrata resin of the present invention can also improve the penetration effect of the python oil composition. Comparative Example 1 changes the extraction method of python oil, resulting in a poor penetration effect of the python oil extract, and Comparative Example 2 changes the components of the composition, resulting in a poor penetration effect of the finished product.

[0109] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A python oil composition having the function of repairing skin after sunburn, characterized in that: The composition comprises, by weight percentage, 8-12% python oil extract, 0.2-0.5% allantoin, 25-30% glycerol, 2-4% carboxymethyl deacetylated chitosan, 0.05-0.3% sodium hyaluronate and the balance water; The preparation method of the python oil extract comprises the following steps: (1) freeze-drying snake fat, and crushing the frozen snake fat to obtain fat powder; (2) extracting the fat powder using an extraction kettle at an extraction temperature of 35-45° C., an extraction pressure of 0.3-1.0 MPa, and an extraction time of 120-150 min to obtain a crude snake oil; (3) adding the primary crude snake oil into a decomposition kettle at a decomposition temperature of 90-100° C. and a decomposition pressure of 0.2 MPa-0.4 MPa to obtain secondary crude snake oil; (4) adding a mixed enzyme to the secondary crude snake oil for enzymolysis to obtain enzymolyzed snake oil; (5) filtering the enzymatically hydrolyzed snake oil using a macroporous resin to obtain a python oil extract; The mixed enzyme comprises trypsin and Rhizomucor miehei lipase in a mass ratio of 2-5:

1.

2. The python oil composition having the function of repairing skin after sunburn according to claim 1, characterized in that: The invention also comprises 0.04-0.08% of Boswellia serrata resin extract by weight.

3. The python oil composition having the function of repairing skin after sunburn according to claim 1, characterized in that: In step (1), the freeze-drying time is 10-12 hours and the freezing temperature is -45 to -50 degrees.

4. The python oil composition having the function of repairing skin after sunburn according to claim 1, characterized in that: In step (2), the extractant is dichloromethane, and the dichloromethane flow rate is 4-6 kg / hr.

5. The python oil composition having the function of repairing skin after sunburn according to claim 1, characterized in that: In step (4), the mass ratio of the mixed enzyme to the secondary crude snake oil is 0.03-0.05:

10.

6. The python oil composition having the function of repairing skin after sunburn according to claim 1, characterized in that: In step (5), the macroporous resin is filtered by eluting with 5-8 BV of an ethanol solution with a concentration of 50-70 wt%, and then eluting with 10-15 BV of an ethanol solution with a concentration of 30-40 wt%.

7. The method for preparing the python oil composition having the function of repairing skin after sunburn according to claim 1, characterized in that: The following steps are involved: S1: mixing allantoin, glycerol, carboxymethyl chitosan and sodium hyaluronate to prepare component A; S2: Add water to component A and stir at a stirring temperature of 70-80°C, stirring at 50-60 r / min for 15-25 min, and stirring again at 20-40 r / min for 10-15 min to obtain component B; S3: Add python oil extract and frankincense resin extract to component B, stir at 15-25 r / min for 3-5 min at 50-60° C., and keep warm for 20-30 min to obtain a finished product.