Method for preparing cold-soluble instant ampelopsis grossedentata powder with different dihydromyricetin contents

Abstract

The invention provides a method for preparing cold-soluble instant ampelopsis grossedentata powder with different dihydromyricetin contents. The method comprises the following steps: S1, raw material treatment: drying and crushing ampelopsis grossedentata raw materials; s2, extracting for several times: respectively extracting the ampelopsis grossedentata raw material in a solvent at the temperature of 85 DEG C according to the mass ratio of 1: 11: 1: 9: 1: 5, and extracting for 20-30 minutes each time to obtain feed liquid; according to the method, dihydromyricetin can be better separated out, the content of the obtained precipitate dihydromyricetin is higher, meanwhile, the ampelopsis grossedentata powder with the content of dihydromyricetin being lower than 25% can be obtained, the ampelopsis grossedentata powder has an extremely good cold dissolving effect, no precipitate is separated out in the storage process, the stability of the ampelopsis grossedentata powder during storage is improved, the shelf life of the ampelopsis grossedentata powder during storage is prolonged, and the ampelopsis grossedentata powder is improved. The situation that the ampelopsis grossedentata powder is easily affected with damp, oxidized or deteriorated during storage is avoided, dihydromyricetin can be effectively extracted, and the deep processing application range of the ampelopsis grossedentata can be remarkably expanded.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of rattan tea powder, and in particular to a method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents. Background Art

[0002] Tengcha is the branches and leaves of the large-leafed snake grape of the grape family. It contains a variety of trace elements, as well as flavonoids such as flavonoids, flavonols, isoflavones, flavanones, chalcones and anthocyanins. Its content of brass compounds and selenium is much higher than that of green tea and scented tea. Tengcha has the effects of protecting cardiovascular, anti-oxidation, protecting liver, and sobering up. Because tengcha contains a large amount of flavonoids, it has a very high scavenging rate for free radicals, which can reduce oxidative damage in the body and has anti-aging effects. Drinking a little tengcha in daily life can delay aging.

[0003] Dihydromyricetin is the flavonoid compound with the highest content in rattan tea, but when the dihydromyricetin content in rattan tea powder is too high (the dihydromyricetin content in rattan tea powder is 25% and above), it is easy for the rattan tea powder to be damp, oxidized or deteriorate during storage, affecting the stability and shelf life of the rattan tea powder; and the precipitate produced in the preparation process of rattan tea powder contains a certain content of dihydromyricetin, but the precipitate has not undergone further treatment and is generally treated as waste, resulting in a waste of dihydromyricetin. Summary of the invention

[0004] The invention discloses a method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents, which solves the problems that the content of dihydromyricetin in rattan tea powder is too high, which easily causes the rattan tea powder to be damp, oxidized or deteriorated during storage, and the precipitate containing dihydromyricetin cannot be effectively utilized.

[0005] In order to solve the above technical problems, the present invention specifically adopts the following technical solutions:

[0006] A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents, comprising the following steps:

[0007] S1, raw material processing: drying and crushing the rattan tea raw materials;

[0008] S2, extracting in batches: extracting the rattan tea raw material in solvents at a mass ratio of 1:11 / 1:9 / 1:5 at a temperature of 85°C, each time for 20 to 30 minutes, to obtain a feed solution;

[0009] S3, filtration and sedimentation: the feed liquid is directly filtered without cooling, and the filtered liquid is placed in a cooling barrel at 5-10°C, cooled for 6 hours, and the precipitate and the supernatant are separated; the precipitate is recrystallized by adding water to obtain a crystal; the crystal is then dried at low temperature to obtain a product with a dihydromyricetin content, and the supernatant is reserved for later use;

[0010] S4, preparation and sterilization: adding 10-20% of β-cyclodextrin and 90-230% of maltodextrin according to the solid content of the supernatant to fully dissolve to obtain a preparation solution, and concentrating the preparation solution, sterilizing the concentrated solution under high temperature conditions and then cooling it to 25-30° C.;

[0011] S5, drying: drying the sterilized concentrated solution to obtain cold-soluble instant rattan tea powder.

[0012] Preferably, in step S1, the raw material of rattan tea is old and tender leaves, crushed powder or tender stems of rattan tea that have undergone primary processing; the degree of drying is that the moisture content is less than 8%; the degree of crushing is less than 60 mesh and the content of crushed powder accounts for less than 10%.

[0013] Preferably, in step S2, the solvent is pure water with a resistivity of less than 10 MΩ*cm, the first solid-liquid ratio in the extraction process is 1:11, and the extraction time is 30 min; the second solid-liquid ratio is 1:9, and the extraction time is 30 min; the third solid-liquid ratio is 1:5, and the extraction time is 30 min; the three extraction temperatures are all extracted at 85°C.

[0014] Preferably, in step S3, the three extracted solutions are filtered while hot, and the temperature is maintained at 50-60° C. during the filtration process.

[0015] Preferably, in step S3, a tubular centrifuge is used for filtration, and the centrifugal flow rate of the tubular centrifuge is 300 L / hr.

[0016] Preferably, in step S3, the filtrate is cooled rapidly to 5-10° C. in a cooling barrel by means of a coil; cooled for 6 hours; a precipitate and a supernatant are separated by a horizontal centrifuge; the precipitate is dissolved in water, cooled and recrystallized to obtain a crystal; the crystal is dried at a low temperature of 40-60° C. to obtain a dihydromyricetin product with a content of 25%-90%; and the supernatant is reserved for later use.

[0017] Preferably, in step S4, 10-20% of β-cyclodextrin and 90-230% of maltodextrin are added to the supernatant according to the solid content to fully dissolve to obtain a prepared solution; and the prepared solution is concentrated using a triple-effect vacuum concentrator to a concentration of 25-30% Brix.

[0018] Preferably, the method includes step S4, using a tube-in-tube or coil UHT instantaneous high temperature sterilization device, controlling the sterilization temperature to 115-127° C., the sterilization time to 10-15 seconds, and cooling to 25-30° C.

[0019] Preferably, in step S5, a pressure spray drying tower is used for drying, the homogenization pressure is controlled to be 20 MPa, the temperature in the tower is 90° C., and the exhaust temperature is 85° C. After drying, the cold-soluble instant rattan tea powder can be obtained.

[0020] Preferably, in step S5, the content of dihydromyricetin in the Ampelopsis ulmoides powder is less than 25%.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The selectivity of raw materials is wider. The old and tender leaves, crushed powder or tender stems of the rattan tea that have been primarily processed can be used as production raw materials. Through multiple extractions and cooling precipitation, dihydromyricetin can be better precipitated, and the obtained precipitate has a higher dihydromyricetin content. At the same time, rattan tea powder with a dihydromyricetin content of less than 25% can also be obtained, so that the rattan tea powder has an excellent cold-dissolving effect, and no precipitate is precipitated during storage, thereby improving the stability and shelf life of the rattan tea powder during storage, avoiding the rattan tea powder from being easily affected by moisture, oxidation or deterioration during storage, and can effectively extract dihydromyricetin, which can significantly expand the deep processing application scope of rattan tea. DETAILED DESCRIPTION

[0023] The specific contents of the present invention are described in detail below in conjunction with embodiments.

[0024] Embodiment 1:

[0025] S1, raw material processing, selecting old and young leaves, crushed powder or young stems of rattan tea that have been processed; the degree of drying is less than 8% of moisture content; the degree of crushing is less than 60 mesh and the proportion of crushed powder is less than 10%;

[0026] S2, extracting in batches, preparing pure water with a resistivity of less than 10MΩ*cm as a solvent, taking 1kg of rattan tea raw material and 11kg of solvent, mixing them evenly, extracting at a temperature of 85°C for 30 minutes to obtain extract A; then mixing the extracted rattan tea raw material with 9kg of solvent, performing a second extraction at a temperature of 85°C, and obtaining extract B after 30 minutes; then mixing the rattan tea raw material after the second extraction with 5kg of solvent, performing a third extraction at a temperature of 85°C, and obtaining extract C after 25 minutes; mixing extract A, extract B and extract C to obtain a feed liquid;

[0027] S3, filtering and settling, without cooling the feed liquid, directly using a tubular centrifuge for filtration, the centrifugal flow rate of the tubular centrifuge is 300L / hr, and the temperature of the feed liquid is maintained at 55°C during filtration; the filtered feed liquid is cooled in a cooling barrel through a coil to quickly cool the temperature to 8°C, and after cooling for 6 hours, a horizontal centrifuge is used to separate the precipitate and the supernatant; the precipitate is dissolved in water, cooled, and recrystallized to obtain a crystal, and the crystal is low-temperature dried in an environment of 60°C to obtain a dihydromyricetin product with a content of 85.6%, and the supernatant is reserved for later use;

[0028] S4, preparation and sterilization, add 20% β-cyclodextrin and 230% maltodextrin to the supernatant according to the solid content, fully dissolve, and obtain the preparation liquid, and then use a triple-effect vacuum concentration device to concentrate the preparation liquid to a concentration of 26% Brix; then use a tube or coil UHT instantaneous high temperature sterilization equipment to sterilize the concentrate, control the sterilization temperature to 127°C, the sterilization time to 15s, and cool to 30°C;

[0029] S5, drying, using a pressure spray drying tower for drying, controlling the homogenization pressure to 20 MPa, the temperature in the tower to 90° C., and the exhaust temperature to 85° C., after drying, a cold-soluble instant rattan tea powder with a dihydromyricetin content of 18.8% can be obtained.

[0030] Embodiment 2:

[0031] S1, raw material processing, selecting old and young leaves, crushed powder or young stems of rattan tea that have been processed; the degree of drying is less than 8% of moisture content; the degree of crushing is less than 60 mesh and the proportion of crushed powder is less than 10%;

[0032] S2, extracting in batches, preparing pure water with a resistivity of less than 10MΩ*cm as a solvent, taking 1kg of rattan tea raw material and 11kg of solvent, mixing them evenly, extracting them at a temperature of 85°C for 20 minutes to obtain extract A; then mixing the extracted rattan tea raw material with 9kg of solvent, performing a second extraction at a temperature of 85°C, and obtaining extract B after 200 minutes; then mixing the rattan tea raw material after the second extraction with 5kg of solvent, performing a third extraction at a temperature of 85°C, and obtaining extract C after 20 minutes; mixing extract A, extract B and extract C to obtain a feed liquid;

[0033] S3, filtering and settling, without cooling the feed liquid, directly using a tubular centrifuge for filtration, the centrifugal flow rate of the tubular centrifuge is 300L / hr, and the temperature of the feed liquid is maintained at 50°C during filtration; the filtered feed liquid is cooled in a cooling barrel by a coil to quickly cool the temperature to 5°C, and after cooling for 6 hours, a precipitate and a supernatant are separated by a horizontal centrifuge; the precipitate is dissolved in water, cooled, and recrystallized, and after obtaining a crystal, the crystal is low-temperature dried in an environment of 50°C, and then a dihydromyricetin product with a content of 78.3% is obtained, and the supernatant is reserved for later use;

[0034] S4, preparation and sterilization, add 20% β-cyclodextrin and 230% maltodextrin to the supernatant according to the solid content, fully dissolve, and obtain the preparation liquid, and then use a triple-effect vacuum concentration device to concentrate the preparation liquid to a concentration of 26% Brix; then use a tube or coil UHT instantaneous high temperature sterilization equipment to sterilize the concentrate, control the sterilization temperature to 127°C, the sterilization time to 15s, and cool to 30°C;

[0035] S5, drying, using a pressure spray drying tower for drying, controlling the homogenization pressure to 20 MPa, the temperature in the tower to 90° C., and the exhaust temperature to 85° C., after drying, a cold-soluble instant rattan tea powder with a dihydromyricetin content of 19.6% can be obtained.

[0036] Table 1

[0037]

[0038] According to Table 1, the content of dihydromyricetin in the instant rattan tea powder prepared by the present invention is less than 25%, so that the rattan tea powder has an excellent cold dissolution effect, and no precipitate is precipitated during storage, thereby improving the stability and shelf life of the rattan tea powder during storage; and a dihydromyricetin product with a content of 25%-90% can also be prepared, expanding the deep processing application scope of rattan tea.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents, characterized in that: The following steps are involved: S1, raw material processing: drying and crushing the rattan tea raw materials; S2, extracting in batches: extracting the rattan tea raw material in solvents at a mass ratio of 1:11 / 1:9 / 1:5 at a temperature of 85°C, each time for 20 to 30 minutes, to obtain a feed solution; S3, filtration and sedimentation: the feed liquid is directly filtered without cooling, and the filtered liquid is placed in a cooling barrel at 5-10°C, cooled for 6 hours, and the precipitate and the supernatant are separated; the precipitate is recrystallized by adding water to obtain a crystal; the crystal is then dried at low temperature to obtain a product with a dihydromyricetin content, and the supernatant is reserved for later use; S4, preparation and sterilization: adding 10-20% of β-cyclodextrin and 90-230% of maltodextrin according to the solid content of the supernatant to fully dissolve to obtain a preparation solution, and concentrating the preparation solution, sterilizing the concentrated solution under high temperature conditions and then cooling it to 25-30° C.; S5, drying: drying the sterilized concentrated solution to obtain cold-soluble instant rattan tea powder.

2. A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: In step S1, the raw material of rattan tea is old and tender leaves, crushed powder or tender stems of rattan tea that have undergone primary processing; the degree of drying is that the moisture content is less than 8%; the degree of crushing is that the content of crushed powder less than 60 mesh accounts for less than 10%.

3. A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: In step S2, the solvent is pure water with a resistivity of less than 10MΩ*cm. During the extraction process, the first solid-liquid ratio is 1:11, and the extraction time is 30 minutes; the second solid-liquid ratio is 1:9, and the extraction time is 30 minutes; the third solid-liquid ratio is 1:5, and the extraction time is 30 minutes; the three extraction temperatures are all 85°C.

4. The method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: In the step S3, the three extracted liquids are filtered while hot, and the temperature is maintained at 50-60°C during the filtration process.

5. A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 4, characterized in that: In the step S3, a tubular centrifuge is used for filtration, and the centrifugal flow rate of the tubular centrifuge is 300 L / hr.

6. A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 5, characterized in that: In the step S3, the filtrate is cooled rapidly to 5-10° C. in a cooling barrel by means of a coil; cooled for 6 hours; a precipitate and a supernatant are separated by a horizontal centrifuge; the precipitate is dissolved in water, cooled and recrystallized to obtain a crystal; the crystal is dried at a low temperature of 40-60° C. to obtain a dihydromyricetin product with a content of 25%-90%; and the supernatant is reserved for later use.

7. A method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: In step S4, 10-20% of β-cyclodextrin and 90-230% of maltodextrin are added to the supernatant according to the solid content to fully dissolve and obtain a prepared solution; the prepared solution is concentrated using a triple-effect vacuum concentrator to a concentration of 25-30% Brix.

8. The method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: The method includes step S4, using a tube-in-tube or coil UHT instantaneous high temperature sterilization device, controlling the sterilization temperature to 115-127° C., the sterilization time to 10-15 seconds, and cooling to 25-30° C.

9. The method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: In step S5, a pressure spray drying tower is used for drying, the homogenization pressure is controlled to be 20 MPa, the temperature in the tower is 90° C., and the exhaust temperature is 85° C. After drying, the cold-soluble instant rattan tea powder can be obtained.

10. The method for preparing cold-soluble instant rattan tea powder with different dihydromyricetin contents according to claim 1, characterized in that: In the step S5, the content of dihydromyricetin in the rattan tea powder is less than 25%.

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